AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1284640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 93 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGTTTAATTGACAATATT+TGG 0.125399 8_3:+27115761 None:intergenic
ATTAAAACTTAAGAATTAAT+TGG 0.153202 8_3:-27112693 Msa1284640:three_prime_UTR
AACGGAATGGGTTAAAATTA+TGG 0.195683 8_3:+27115550 None:intergenic
AAAATTGGGTTTCATGGTTT+TGG 0.199945 8_3:+27115711 None:intergenic
GTGAGATTGAGGAATAAAAT+TGG 0.236352 8_3:+27115696 None:intergenic
GAAGGGAAGCATGTGTTTAT+TGG 0.254125 8_3:-27115177 Msa1284640:three_prime_UTR
TTTGTAGACCTTCAAGTTAT+AGG 0.264756 8_3:-27112661 Msa1284640:three_prime_UTR
AAGCATGTGTTTATTGGATT+AGG 0.266751 8_3:-27115171 Msa1284640:three_prime_UTR
AGGAATAAAATTGGGTTTCA+TGG 0.267495 8_3:+27115705 None:intergenic
ACTCTTCACATTAATGCTTT+TGG 0.278094 8_3:-27115784 None:intergenic
CAAGTTATAGGTTATCCTAT+TGG 0.318712 8_3:-27112649 Msa1284640:three_prime_UTR
AGCATGTGTTTATTGGATTA+GGG 0.332772 8_3:-27115170 Msa1284640:three_prime_UTR
CTTGTCCTATATATATGTTT+TGG 0.337556 8_3:+27115446 None:intergenic
TTAGGCAAATCAACAATTGC+TGG 0.347016 8_3:-27112762 Msa1284640:intron
TTCTTCCAAAACATATATAT+AGG 0.349435 8_3:-27115451 Msa1284640:CDS
CTTCTAAAGATTGTGTATAA+TGG 0.357548 8_3:-27112901 Msa1284640:intron
GAGCAACTGTAGAAAAGAAA+TGG 0.357579 8_3:+27115482 None:intergenic
TTAAAACTTAAGAATTAATT+GGG 0.366320 8_3:-27112692 Msa1284640:three_prime_UTR
GGTGAGAGCTTAAATGAAAC+TGG 0.376751 8_3:+27115572 None:intergenic
TGAGATTGAGGAATAAAATT+GGG 0.377573 8_3:+27115697 None:intergenic
AATACCTATGACTTGCGTTT+TGG 0.377633 8_3:+27113899 None:intergenic
AGAAGCTAATGACCAACTTA+AGG 0.395849 8_3:-27113073 Msa1284640:three_prime_UTR
ACGGAATGGGTTAAAATTAT+GGG 0.402922 8_3:+27115551 None:intergenic
TGTGGGAACGAGACTGGAAT+TGG 0.409476 8_3:+27115737 None:intergenic
AGTATTATCAATCATCCAAT+AGG 0.414360 8_3:+27112634 None:intergenic
TGCTGTTAATCATAGGCTTA+AGG 0.419472 8_3:-27115041 Msa1284640:intron
TGGAGTTGTGGGAACGAGAC+TGG 0.421945 8_3:+27115731 None:intergenic
AACTGAACCCTCACACTGAC+CGG 0.431437 8_3:+27115251 None:intergenic
TGTGAGAAGAGAACATGATA+AGG 0.442504 8_3:-27115088 Msa1284640:three_prime_UTR
GAAGCTAATGACCAACTTAA+GGG 0.443177 8_3:-27113072 Msa1284640:intron
GCTTAAATGAAACTGGATAA+AGG 0.443636 8_3:+27115579 None:intergenic
TTTCTGCTTAAGATTGAAGA+TGG 0.446632 8_3:-27115201 Msa1284640:three_prime_UTR
AGCGAAGAGATCGCCGGAGT+TGG 0.447638 8_3:+27115290 None:intergenic
GCGTTGTGTCCCGCCGTGAC+CGG 0.447886 8_3:-27115335 Msa1284640:CDS
GTGTGAGGGTTCAGTTGAAC+TGG 0.448765 8_3:-27115244 Msa1284640:three_prime_UTR
TAGGATAACCTATAACTTGA+AGG 0.448867 8_3:+27112653 None:intergenic
ACTGCCAAAACGCAAGTCAT+AGG 0.449501 8_3:-27113903 Msa1284640:intron
GCGAAGAGATCGCCGGAGTT+GGG 0.452093 8_3:+27115291 None:intergenic
GTTTATTGGATTAGGGTTAA+CGG 0.452324 8_3:-27115163 Msa1284640:three_prime_UTR
CGCCGGAGGTTGTTCGTGGC+GGG 0.452665 8_3:+27115412 None:intergenic
AAAGGCATGTGTTACGTCAA+TGG 0.457053 8_3:-27112952 Msa1284640:three_prime_UTR
GCCATCCTGCTGTTAATCAT+AGG 0.463255 8_3:-27115048 Msa1284640:three_prime_UTR
GGTTCCAATCAGACAAGATC+TGG 0.463783 8_3:-27115371 Msa1284640:CDS
AGAGAACATGATAAGGAGTC+TGG 0.464167 8_3:-27115081 Msa1284640:three_prime_UTR
GCCGTGACCGGTGTGAGATA+CGG 0.467324 8_3:-27115323 Msa1284640:CDS
GGACACAACGCGGAGACGGC+CGG 0.477578 8_3:+27115347 None:intergenic
GCCACGGCACAGTGAGATTG+AGG 0.477789 8_3:+27115669 None:intergenic
ATCCCGCCACGAACAACCTC+CGG 0.483393 8_3:-27115414 Msa1284640:CDS
TAACCGCCGGAGGTTGTTCG+TGG 0.487228 8_3:+27115408 None:intergenic
ATTGAGGAAAAGTGAGATTG+AGG 0.491826 8_3:+27115685 None:intergenic
CGGACCAGATCTTGTCTGAT+TGG 0.492781 8_3:+27115367 None:intergenic
TAAAGTCATTACCCTTAAGT+TGG 0.492910 8_3:+27113061 None:intergenic
TTTCAATTTGAATTGTACTC+TGG 0.494796 8_3:-27112566 Msa1284640:three_prime_UTR
GCGGTTACAAATGCGGCTAA+TGG 0.502661 8_3:-27115392 Msa1284640:CDS
AGAAAAGAAATGGGTCGAAA+AGG 0.503425 8_3:+27115492 None:intergenic
CAATCAGACAAGATCTGGTC+CGG 0.506501 8_3:-27115366 Msa1284640:CDS
GGCGGGACACAACGCGGAGA+CGG 0.509066 8_3:+27115343 None:intergenic
GATTAACAGCAGGATGGCAT+TGG 0.514115 8_3:+27115053 None:intergenic
AGCAACTGTAGAAAAGAAAT+GGG 0.514548 8_3:+27115483 None:intergenic
AGTGAAGAACGGTGGAGAAA+CGG 0.515942 8_3:+27115532 None:intergenic
CTTAAGATTGAAGATGGAGA+AGG 0.523506 8_3:-27115195 Msa1284640:three_prime_UTR
AGATTTAGCCTGTCGAGTGA+TGG 0.527799 8_3:-27113932 Msa1284640:three_prime_UTR
ACCGTATCTCACACCGGTCA+CGG 0.531370 8_3:+27115322 None:intergenic
CATAATCCTTCGATTGAACA+AGG 0.536572 8_3:+27115604 None:intergenic
TATCTCACACCGGTCACGGC+GGG 0.540870 8_3:+27115326 None:intergenic
GTAATGTTCCATCACTCGAC+AGG 0.545474 8_3:+27113924 None:intergenic
ACAGGAGTTGAAGTAGAGAA+AGG 0.547321 8_3:-27113121 Msa1284640:intron
TCCTCAATCTCACTGTGCCG+TGG 0.558608 8_3:-27115670 Msa1284640:five_prime_UTR
ACTGAACCCTCACACTGACC+GGG 0.558637 8_3:+27115252 None:intergenic
GAACGGTGGAGAAACGGAAT+GGG 0.566786 8_3:+27115538 None:intergenic
GACCACCCTTGTTCAATCGA+AGG 0.569740 8_3:-27115610 Msa1284640:five_prime_UTR
CCGCCGGAGGTTGTTCGTGG+CGG 0.571890 8_3:+27115411 None:intergenic
TCAACAATTGCTGGTGTACG+TGG 0.590971 8_3:-27112753 Msa1284640:three_prime_UTR
AGAACGGTGGAGAAACGGAA+TGG 0.592306 8_3:+27115537 None:intergenic
TTGGATTAGGGTTAACGGAG+AGG 0.594029 8_3:-27115158 Msa1284640:three_prime_UTR
TTAAGATTGAAGATGGAGAA+GGG 0.597144 8_3:-27115194 Msa1284640:three_prime_UTR
TTAAGCCTATGATTAACAGC+AGG 0.600310 8_3:+27115043 None:intergenic
GCCGCATTTGTAACCGCCGG+AGG 0.612310 8_3:+27115398 None:intergenic
ACCTCCGGCGGTTACAAATG+CGG 0.616884 8_3:-27115399 Msa1284640:CDS
TTAGCCGCATTTGTAACCGC+CGG 0.625611 8_3:+27115395 None:intergenic
ACACGCAGCGAAGAGATCGC+CGG 0.626051 8_3:+27115284 None:intergenic
TAGCCACGGTTATTTAGCCA+CGG 0.636577 8_3:+27115653 None:intergenic
GCCTATGATTAACAGCAGGA+TGG 0.640269 8_3:+27115047 None:intergenic
ATCCTTCGATTGAACAAGGG+TGG 0.644361 8_3:+27115608 None:intergenic
TTCAGCACTGCTAAGACTCG+AGG 0.650870 8_3:-27112721 Msa1284640:three_prime_UTR
CGGTTAGAAGATCCCAACTC+CGG 0.656754 8_3:-27115303 Msa1284640:exon
CAACAAAGAGTGAAGAACGG+TGG 0.678077 8_3:+27115524 None:intergenic
CTTCTAACCGTATCTCACAC+CGG 0.687609 8_3:+27115316 None:intergenic
GAACAACAAAGAGTGAAGAA+CGG 0.690550 8_3:+27115521 None:intergenic
CCGCCACGAACAACCTCCGG+CGG 0.697916 8_3:-27115411 Msa1284640:CDS
GGTCACGGCGGGACACAACG+CGG 0.706808 8_3:+27115337 None:intergenic
ATAATCCTTCGATTGAACAA+GGG 0.716702 8_3:+27115605 None:intergenic
GTATCTCACACCGGTCACGG+CGG 0.748302 8_3:+27115325 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAACTTAAGAATTAAT+TGG - chr8_3:27115581-27115600 Msa1284640:CDS 10.0%
!! TTAAAACTTAAGAATTAATT+GGG - chr8_3:27115582-27115601 Msa1284640:CDS 10.0%
!! AAAGTTTAATTGACAATATT+TGG + chr8_3:27112516-27112535 None:intergenic 15.0%
!!! ACTATTTTTCAACTAAAAAT+TGG + chr8_3:27113322-27113341 None:intergenic 15.0%
!! AACTTAAACTTTGTAAAAAT+AGG - chr8_3:27113415-27113434 Msa1284640:intron 15.0%
!! TACAATTAGAAAATTGAATA+AGG + chr8_3:27114012-27114031 None:intergenic 15.0%
!! TTATAAAAAAATGAGACATA+AGG - chr8_3:27114070-27114089 Msa1284640:intron 15.0%
!! TATAAAAAAATGAGACATAA+GGG - chr8_3:27114071-27114090 Msa1284640:intron 15.0%
!!! TATATATGTTTTGGAAGAAA+AGG + chr8_3:27112822-27112841 None:intergenic 20.0%
!! TTCTTCCAAAACATATATAT+AGG - chr8_3:27112823-27112842 Msa1284640:intron 20.0%
!!! CTTTATTTCGAATTTGTTAA+TGG - chr8_3:27115027-27115046 Msa1284640:intron 20.0%
!!! AACAATTTTTATGCCATTAA+CGG - chr8_3:27115270-27115289 Msa1284640:three_prime_UTR 20.0%
!!! ATTTTGTACAAAAAAACACA+AGG + chr8_3:27115475-27115494 None:intergenic 20.0%
!!! GTTAGATTTTATGTTTTTGA+TGG - chr8_3:27115673-27115692 Msa1284640:five_prime_UTR 20.0%
! TGAGATTGAGGAATAAAATT+GGG + chr8_3:27112580-27112599 None:intergenic 25.0%
!! CTTGTCCTATATATATGTTT+TGG + chr8_3:27112831-27112850 None:intergenic 25.0%
!! TATGCATAAAATTTGTTTCC+AGG - chr8_3:27113379-27113398 Msa1284640:intron 25.0%
!! ATGCATAAAATTTGTTTCCA+GGG - chr8_3:27113380-27113399 Msa1284640:intron 25.0%
!! TATTTCTTAGTTGGTACATT+TGG - chr8_3:27113625-27113644 Msa1284640:intron 25.0%
!! ATTTCTTAGTTGGTACATTT+GGG - chr8_3:27113626-27113645 Msa1284640:intron 25.0%
! TGTTACTATAGTTGAATACA+AGG - chr8_3:27113731-27113750 Msa1284640:intron 25.0%
! CTAATATGTGAATAATCAAG+CGG - chr8_3:27113835-27113854 Msa1284640:intron 25.0%
!! TAATATGACTTGTGAAATGA+AGG - chr8_3:27114037-27114056 Msa1284640:intron 25.0%
!! AATATGACTTGTGAAATGAA+GGG - chr8_3:27114038-27114057 Msa1284640:intron 25.0%
!! ACATTTTACTGAAGAAATTC+AGG + chr8_3:27114689-27114708 None:intergenic 25.0%
!!! TATATATGCTTTGTTTTGAC+AGG + chr8_3:27114777-27114796 None:intergenic 25.0%
! CAAAGCATATATATACGTAT+TGG - chr8_3:27114784-27114803 Msa1284640:intron 25.0%
! CTGAAATTAGAGTTCAAAAA+GGG - chr8_3:27115075-27115094 Msa1284640:three_prime_UTR 25.0%
!! CTTCTAAAGATTGTGTATAA+TGG - chr8_3:27115373-27115392 Msa1284640:CDS 25.0%
!! TTTTGTACAAAATCAAACCT+TGG - chr8_3:27115482-27115501 Msa1284640:CDS 25.0%
! AGTATTATCAATCATCCAAT+AGG + chr8_3:27115643-27115662 None:intergenic 25.0%
!!! TATGTTTTTGATGGAACTAT+TGG - chr8_3:27115682-27115701 Msa1284640:five_prime_UTR 25.0%
! TTTCAATTTGAATTGTACTC+TGG - chr8_3:27115708-27115727 Msa1284640:five_prime_UTR 25.0%
! AAAATTGGGTTTCATGGTTT+TGG + chr8_3:27112566-27112585 None:intergenic 30.0%
AGGAATAAAATTGGGTTTCA+TGG + chr8_3:27112572-27112591 None:intergenic 30.0%
GTGAGATTGAGGAATAAAAT+TGG + chr8_3:27112581-27112600 None:intergenic 30.0%
ATAATCCTTCGATTGAACAA+GGG + chr8_3:27112672-27112691 None:intergenic 30.0%
GCTTAAATGAAACTGGATAA+AGG + chr8_3:27112698-27112717 None:intergenic 30.0%
!! ACGGAATGGGTTAAAATTAT+GGG + chr8_3:27112726-27112745 None:intergenic 30.0%
!! AACGGAATGGGTTAAAATTA+TGG + chr8_3:27112727-27112746 None:intergenic 30.0%
AGCAACTGTAGAAAAGAAAT+GGG + chr8_3:27112794-27112813 None:intergenic 30.0%
TTTCTGCTTAAGATTGAAGA+TGG - chr8_3:27113073-27113092 Msa1284640:three_prime_UTR 30.0%
TTAAGATTGAAGATGGAGAA+GGG - chr8_3:27113080-27113099 Msa1284640:three_prime_UTR 30.0%
AAGCATGTGTTTATTGGATT+AGG - chr8_3:27113103-27113122 Msa1284640:three_prime_UTR 30.0%
AGCATGTGTTTATTGGATTA+GGG - chr8_3:27113104-27113123 Msa1284640:three_prime_UTR 30.0%
! GTTTATTGGATTAGGGTTAA+CGG - chr8_3:27113111-27113130 Msa1284640:three_prime_UTR 30.0%
! TCTCATGTTTGTGAACTTTT+AGG - chr8_3:27113473-27113492 Msa1284640:intron 30.0%
TTGCTGTGATATTTCTTAGT+TGG - chr8_3:27113616-27113635 Msa1284640:intron 30.0%
AGTAACACATGTAAAGAGAA+GGG + chr8_3:27113718-27113737 None:intergenic 30.0%
TAGTAACACATGTAAAGAGA+AGG + chr8_3:27113719-27113738 None:intergenic 30.0%
GTCTTAAAAGACAACACAAA+TGG - chr8_3:27113810-27113829 Msa1284640:intron 30.0%
GTTGCAAGCTATAAACTATA+AGG - chr8_3:27114222-27114241 Msa1284640:intron 30.0%
CAGAAAATTGAATGAACAGA+CGG + chr8_3:27114311-27114330 None:intergenic 30.0%
TTATAATACGTACTCTGTGT+CGG + chr8_3:27114489-27114508 None:intergenic 30.0%
CAATTTCTTCTGTCAATATC+TGG - chr8_3:27114581-27114600 Msa1284640:intron 30.0%
AACTAATGAAGTTACCTCTT+GGG + chr8_3:27114952-27114971 None:intergenic 30.0%
AAACTAATGAAGTTACCTCT+TGG + chr8_3:27114953-27114972 None:intergenic 30.0%
AAGAGGTAACTTCATTAGTT+TGG - chr8_3:27114952-27114971 Msa1284640:intron 30.0%
GCTGAAATTAGAGTTCAAAA+AGG - chr8_3:27115074-27115093 Msa1284640:three_prime_UTR 30.0%
TAAAGTCATTACCCTTAAGT+TGG + chr8_3:27115216-27115235 None:intergenic 30.0%
TCTGCTTTGATAGAGAAAAA+AGG - chr8_3:27115304-27115323 Msa1284640:exon 30.0%
TCAAACCTTGGATATTTCTT+AGG - chr8_3:27115494-27115513 Msa1284640:CDS 30.0%
ATTTGCCTAAGAAATATCCA+AGG + chr8_3:27115502-27115521 None:intergenic 30.0%
! TTTGTAGACCTTCAAGTTAT+AGG - chr8_3:27115613-27115632 Msa1284640:intron 30.0%
TAGGATAACCTATAACTTGA+AGG + chr8_3:27115624-27115643 None:intergenic 30.0%
CAAGTTATAGGTTATCCTAT+TGG - chr8_3:27115625-27115644 Msa1284640:intron 30.0%
!!! TTTCATGGTTTTGGAGTTGT+GGG + chr8_3:27112557-27112576 None:intergenic 35.0%
ATTGAGGAAAAGTGAGATTG+AGG + chr8_3:27112592-27112611 None:intergenic 35.0%
CATAATCCTTCGATTGAACA+AGG + chr8_3:27112673-27112692 None:intergenic 35.0%
GAACAACAAAGAGTGAAGAA+CGG + chr8_3:27112756-27112775 None:intergenic 35.0%
AGAAAAGAAATGGGTCGAAA+AGG + chr8_3:27112785-27112804 None:intergenic 35.0%
GAGCAACTGTAGAAAAGAAA+TGG + chr8_3:27112795-27112814 None:intergenic 35.0%
CTTAAGATTGAAGATGGAGA+AGG - chr8_3:27113079-27113098 Msa1284640:three_prime_UTR 35.0%
TGTGAGAAGAGAACATGATA+AGG - chr8_3:27113186-27113205 Msa1284640:intron 35.0%
TTAAGCCTATGATTAACAGC+AGG + chr8_3:27113234-27113253 None:intergenic 35.0%
TGCTGTTAATCATAGGCTTA+AGG - chr8_3:27113233-27113252 Msa1284640:intron 35.0%
! TAGACATTGGTTAGACACAT+GGG + chr8_3:27113448-27113467 Msa1284650:CDS 35.0%
! ATAGACATTGGTTAGACACA+TGG + chr8_3:27113449-27113468 Msa1284650:CDS 35.0%
ACATGAGACAGTATAGACAT+TGG + chr8_3:27113461-27113480 Msa1284650:exon 35.0%
TATAGTTGAATACAAGGTCG+TGG - chr8_3:27113737-27113756 Msa1284640:intron 35.0%
AGACATAAGGGCTAAAAGAA+AGG - chr8_3:27114083-27114102 Msa1284640:intron 35.0%
TGATATGCATGTTAAGAACG+AGG + chr8_3:27114133-27114152 None:intergenic 35.0%
CAACACATATATCTTTCCTC+AGG + chr8_3:27114195-27114214 None:intergenic 35.0%
!! AATACCTATGACTTGCGTTT+TGG + chr8_3:27114378-27114397 None:intergenic 35.0%
TCATATGCACATTCAGAAAG+AGG + chr8_3:27114418-27114437 None:intergenic 35.0%
ATTGACACACATTCGTAAAG+AGG + chr8_3:27114565-27114584 None:intergenic 35.0%
TTCTTCTGTCAATATCTGGA+TGG - chr8_3:27114585-27114604 Msa1284640:intron 35.0%
! GTCTTTTCTGATATGTTCTG+CGG - chr8_3:27114607-27114626 Msa1284640:intron 35.0%
!!! TTTTAGAACCTACGCTTCAA+TGG + chr8_3:27114659-27114678 None:intergenic 35.0%
AGCTCTCTTGAAATAGTGTT+AGG - chr8_3:27114924-27114943 Msa1284640:intron 35.0%
! AGGTAACTTCATTAGTTTGG+TGG - chr8_3:27114955-27114974 Msa1284640:intron 35.0%
AAAAGGGCTATGATATCATC+AGG - chr8_3:27115091-27115110 Msa1284640:three_prime_UTR 35.0%
AAAGGGCTATGATATCATCA+GGG - chr8_3:27115092-27115111 Msa1284640:three_prime_UTR 35.0%
AGAAGCTAATGACCAACTTA+AGG - chr8_3:27115201-27115220 Msa1284640:three_prime_UTR 35.0%
GAAGCTAATGACCAACTTAA+GGG - chr8_3:27115202-27115221 Msa1284640:three_prime_UTR 35.0%
CTTAAACATGTGTACACCAA+AGG + chr8_3:27115441-27115460 None:intergenic 35.0%
TTAGGCAAATCAACAATTGC+TGG - chr8_3:27115512-27115531 Msa1284640:CDS 35.0%
!!! GTTTCATGGTTTTGGAGTTG+TGG + chr8_3:27112558-27112577 None:intergenic 40.0%
AAATAACCGTGGCTAATCAC+CGG - chr8_3:27112629-27112648 Msa1284640:three_prime_UTR 40.0%
GGTGAGAGCTTAAATGAAAC+TGG + chr8_3:27112705-27112724 None:intergenic 40.0%
! GAAGGGAAGCATGTGTTTAT+TGG - chr8_3:27113097-27113116 Msa1284640:three_prime_UTR 40.0%
AGAGAACATGATAAGGAGTC+TGG - chr8_3:27113193-27113212 Msa1284640:intron 40.0%
!! TTTGTTTCCAGGGTACTGTT+AGG - chr8_3:27113390-27113409 Msa1284640:intron 40.0%
GTAAGACAGTTGAAGAAGTG+TGG + chr8_3:27113677-27113696 Msa1284650:three_prime_UTR 40.0%
CTGTCTTACATCACATCTCA+AGG - chr8_3:27113688-27113707 Msa1284640:intron 40.0%
TTTCAACCAAATGAGCTCAC+AGG + chr8_3:27113772-27113791 None:intergenic 40.0%
CTCATGAAAAAACAGACCAG+AGG + chr8_3:27113873-27113892 None:intergenic 40.0%
ATTCCTTCTAATGCAACCTG+AGG - chr8_3:27114176-27114195 Msa1284640:intron 40.0%
! TTTCCTCAGGTTGCATTAGA+AGG + chr8_3:27114182-27114201 None:intergenic 40.0%
CTCTGATTATCTTTAGCGAC+AGG - chr8_3:27114454-27114473 Msa1284640:intron 40.0%
TTGCAAAACCATTGAAGCGT+AGG - chr8_3:27114648-27114667 Msa1284640:intron 40.0%
TGCTATGCTCATTCAGATAG+AGG + chr8_3:27114714-27114733 None:intergenic 40.0%
AATAGTGTTAGGAACCCAAG+AGG - chr8_3:27114935-27114954 Msa1284640:intron 40.0%
TGATATCATCAGGGTGAGTT+TGG - chr8_3:27115101-27115120 Msa1284640:three_prime_UTR 40.0%
! CTAGTTGTTCTTTTGTCGAC+AGG - chr8_3:27115135-27115154 Msa1284640:three_prime_UTR 40.0%
ACAGGAGTTGAAGTAGAGAA+AGG - chr8_3:27115153-27115172 Msa1284640:three_prime_UTR 40.0%
GATGATGCTTTCACCGTTAA+TGG + chr8_3:27115286-27115305 None:intergenic 40.0%
! AAAGGCATGTGTTACGTCAA+TGG - chr8_3:27115322-27115341 Msa1284640:CDS 40.0%
TGATAGCTTGACATTGCCTT+TGG - chr8_3:27115422-27115441 Msa1284640:CDS 40.0%
TAGCCACGGTTATTTAGCCA+CGG + chr8_3:27112624-27112643 None:intergenic 45.0%
! AGAAAACCGGTGATTAGCCA+CGG + chr8_3:27112638-27112657 None:intergenic 45.0%
ATCCTTCGATTGAACAAGGG+TGG + chr8_3:27112669-27112688 None:intergenic 45.0%
AGTGAAGAACGGTGGAGAAA+CGG + chr8_3:27112745-27112764 None:intergenic 45.0%
CAACAAAGAGTGAAGAACGG+TGG + chr8_3:27112753-27112772 None:intergenic 45.0%
GGTTCCAATCAGACAAGATC+TGG - chr8_3:27112903-27112922 Msa1284640:three_prime_UTR 45.0%
CAATCAGACAAGATCTGGTC+CGG - chr8_3:27112908-27112927 Msa1284640:three_prime_UTR 45.0%
CTTCTAACCGTATCTCACAC+CGG + chr8_3:27112961-27112980 None:intergenic 45.0%
!!! GCTGCGTGTTTTGTTTTTCC+CGG - chr8_3:27113004-27113023 Msa1284640:intron 45.0%
! TTGGATTAGGGTTAACGGAG+AGG - chr8_3:27113116-27113135 Msa1284640:three_prime_UTR 45.0%
GATTAACAGCAGGATGGCAT+TGG + chr8_3:27113224-27113243 None:intergenic 45.0%
GCCATCCTGCTGTTAATCAT+AGG - chr8_3:27113226-27113245 Msa1284640:intron 45.0%
GCCTATGATTAACAGCAGGA+TGG + chr8_3:27113230-27113249 None:intergenic 45.0%
AAGTTTGCCTAACAGTACCC+TGG + chr8_3:27113400-27113419 Msa1284650:CDS 45.0%
TGAATACAAGGTCGTGGCAT+TGG - chr8_3:27113743-27113762 Msa1284640:intron 45.0%
AGATTTAGCCTGTCGAGTGA+TGG - chr8_3:27114342-27114361 Msa1284640:intron 45.0%
GTAATGTTCCATCACTCGAC+AGG + chr8_3:27114353-27114372 None:intergenic 45.0%
ACTGCCAAAACGCAAGTCAT+AGG - chr8_3:27114371-27114390 Msa1284640:intron 45.0%
TCAACAATTGCTGGTGTACG+TGG - chr8_3:27115521-27115540 Msa1284640:CDS 45.0%
!!! ACTTTAAAATAATATTTTAT+TGG - chr8_3:27113921-27113940 Msa1284640:three_prime_UTR 5.0%
!!! TTTTTAACAATATTATATTT+TGG + chr8_3:27114833-27114852 None:intergenic 5.0%
TGTGGGAACGAGACTGGAAT+TGG + chr8_3:27112540-27112559 None:intergenic 50.0%
!!! GTGGCTAATCACCGGTTTTC+TGG - chr8_3:27112637-27112656 Msa1284640:three_prime_UTR 50.0%
GGTGGTCACTACCAGAAAAC+CGG + chr8_3:27112651-27112670 None:intergenic 50.0%
GACCACCCTTGTTCAATCGA+AGG - chr8_3:27112664-27112683 Msa1284640:three_prime_UTR 50.0%
GAACGGTGGAGAAACGGAAT+GGG + chr8_3:27112739-27112758 None:intergenic 50.0%
AGAACGGTGGAGAAACGGAA+TGG + chr8_3:27112740-27112759 None:intergenic 50.0%
TTAGCCGCATTTGTAACCGC+CGG + chr8_3:27112882-27112901 None:intergenic 50.0%
GCGGTTACAAATGCGGCTAA+TGG - chr8_3:27112882-27112901 Msa1284640:intron 50.0%
CGGACCAGATCTTGTCTGAT+TGG + chr8_3:27112910-27112929 None:intergenic 50.0%
CGGTTAGAAGATCCCAACTC+CGG - chr8_3:27112971-27112990 Msa1284640:intron 50.0%
! TGTTTTTCCCGGTCAGTGTG+AGG - chr8_3:27113015-27113034 Msa1284640:intron 50.0%
! GTTTTTCCCGGTCAGTGTGA+GGG - chr8_3:27113016-27113035 Msa1284640:intron 50.0%
AACTGAACCCTCACACTGAC+CGG + chr8_3:27113026-27113045 None:intergenic 50.0%
GTGTGAGGGTTCAGTTGAAC+TGG - chr8_3:27113030-27113049 Msa1284640:intron 50.0%
TGGACAACGACAACACTCGA+CGG + chr8_3:27113657-27113676 Msa1284650:intron 50.0%
TGGTGACCTGTGAGCTCATT+TGG - chr8_3:27113763-27113782 Msa1284640:intron 50.0%
GCGGCATTGCATATTACCTC+TGG - chr8_3:27113854-27113873 Msa1284640:intron 50.0%
GACGGACATGCATGTGCAAA+AGG + chr8_3:27114293-27114312 None:intergenic 50.0%
! CGTACTCTGTGTCGGTACAA+AGG + chr8_3:27114481-27114500 None:intergenic 50.0%
TTCAGCACTGCTAAGACTCG+AGG - chr8_3:27115553-27115572 Msa1284640:CDS 50.0%
TGGAGTTGTGGGAACGAGAC+TGG + chr8_3:27112546-27112565 None:intergenic 55.0%
TCCTCAATCTCACTGTGCCG+TGG - chr8_3:27112604-27112623 Msa1284640:three_prime_UTR 55.0%
GTGCCGTGGCTAAATAACCG+TGG - chr8_3:27112618-27112637 Msa1284640:three_prime_UTR 55.0%
ACCTCCGGCGGTTACAAATG+CGG - chr8_3:27112875-27112894 Msa1284640:intron 55.0%
ACCGTATCTCACACCGGTCA+CGG + chr8_3:27112955-27112974 None:intergenic 55.0%
ACTGAACCCTCACACTGACC+GGG + chr8_3:27113025-27113044 None:intergenic 55.0%
GCCACGGCACAGTGAGATTG+AGG + chr8_3:27112608-27112627 None:intergenic 60.0%
ATCCCGCCACGAACAACCTC+CGG - chr8_3:27112860-27112879 Msa1284640:intron 60.0%
TAACCGCCGGAGGTTGTTCG+TGG + chr8_3:27112869-27112888 None:intergenic 60.0%
TATCTCACACCGGTCACGGC+GGG + chr8_3:27112951-27112970 None:intergenic 60.0%
GTATCTCACACCGGTCACGG+CGG + chr8_3:27112952-27112971 None:intergenic 60.0%
GCCGTGACCGGTGTGAGATA+CGG - chr8_3:27112951-27112970 Msa1284640:three_prime_UTR 60.0%
GCGAAGAGATCGCCGGAGTT+GGG + chr8_3:27112986-27113005 None:intergenic 60.0%
AGCGAAGAGATCGCCGGAGT+TGG + chr8_3:27112987-27113006 None:intergenic 60.0%
ACACGCAGCGAAGAGATCGC+CGG + chr8_3:27112993-27113012 None:intergenic 60.0%
GCCGCATTTGTAACCGCCGG+AGG + chr8_3:27112879-27112898 None:intergenic 65.0%
CGCCGGAGGTTGTTCGTGGC+GGG + chr8_3:27112865-27112884 None:intergenic 70.0%
CCGCCGGAGGTTGTTCGTGG+CGG + chr8_3:27112866-27112885 None:intergenic 70.0%
CCGCCACGAACAACCTCCGG+CGG - chr8_3:27112863-27112882 Msa1284640:intron 70.0%
GGACACAACGCGGAGACGGC+CGG + chr8_3:27112930-27112949 None:intergenic 70.0%
GGCGGGACACAACGCGGAGA+CGG + chr8_3:27112934-27112953 None:intergenic 70.0%
GGTCACGGCGGGACACAACG+CGG + chr8_3:27112940-27112959 None:intergenic 70.0%
GCGTTGTGTCCCGCCGTGAC+CGG - chr8_3:27112939-27112958 Msa1284640:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 27112499 27115797 27112499 ID=Msa1284640;Name=Msa1284640
chr8_3 mRNA 27112499 27115797 27112499 ID=Msa1284640-mRNA-1;Parent=Msa1284640;Name=Msa1284640-mRNA-1;_AED=0.27;_eAED=0.27;_QI=163|0.8|1|1|0|0|6|753|95
chr8_3 exon 27112499 27112780 27112499 ID=Msa1284640-mRNA-1:exon:9401;Parent=Msa1284640-mRNA-1
chr8_3 exon 27112902 27112979 27112902 ID=Msa1284640-mRNA-1:exon:9400;Parent=Msa1284640-mRNA-1
chr8_3 exon 27113073 27113139 27113073 ID=Msa1284640-mRNA-1:exon:9399;Parent=Msa1284640-mRNA-1
chr8_3 exon 27113904 27113952 27113904 ID=Msa1284640-mRNA-1:exon:9398;Parent=Msa1284640-mRNA-1
chr8_3 exon 27115042 27115631 27115042 ID=Msa1284640-mRNA-1:exon:9397;Parent=Msa1284640-mRNA-1
chr8_3 exon 27115660 27115797 27115660 ID=Msa1284640-mRNA-1:exon:9396;Parent=Msa1284640-mRNA-1
chr8_3 five_prime_UTR 27115660 27115797 27115660 ID=Msa1284640-mRNA-1:five_prime_utr;Parent=Msa1284640-mRNA-1
chr8_3 five_prime_UTR 27115607 27115631 27115607 ID=Msa1284640-mRNA-1:five_prime_utr;Parent=Msa1284640-mRNA-1
chr8_3 CDS 27115319 27115606 27115319 ID=Msa1284640-mRNA-1:cds;Parent=Msa1284640-mRNA-1
chr8_3 three_prime_UTR 27115042 27115318 27115042 ID=Msa1284640-mRNA-1:three_prime_utr;Parent=Msa1284640-mRNA-1
chr8_3 three_prime_UTR 27113904 27113952 27113904 ID=Msa1284640-mRNA-1:three_prime_utr;Parent=Msa1284640-mRNA-1
chr8_3 three_prime_UTR 27113073 27113139 27113073 ID=Msa1284640-mRNA-1:three_prime_utr;Parent=Msa1284640-mRNA-1
chr8_3 three_prime_UTR 27112902 27112979 27112902 ID=Msa1284640-mRNA-1:three_prime_utr;Parent=Msa1284640-mRNA-1
chr8_3 three_prime_UTR 27112499 27112780 27112499 ID=Msa1284640-mRNA-1:three_prime_utr;Parent=Msa1284640-mRNA-1
Gene Sequence

>Msa1284640

ATGTTCCTTTATCCAGTTTCATTTAAGCTCTCACCCATAATTTTAACCCATTCCGTTTCTCCACCGTTCTTCACTCTTTGTTGTTCTTCAATCCTTTTCGACCCATTTCTTTTCTACAGTTGCTCAGTTCCTTTTCTTCCAAAACATATATATAGGACAAGATCTACAAGATCCCGCCACGAACAACCTCCGGCGGTTACAAATGCGGCTAATGGTTCCAATCAGACAAGATCTGGTCCGGCCGTCTCCGCGTTGTGTCCCGCCGTGACCGGTGTGAGATACGGTTAG
Protein sequence

>Msa1284640

MFLYPVSFKLSPIILTHSVSPPFFTLCCSSILFDPFLFYSCSVPFLPKHIYRTRSTRSRHEQPPAVTNAANGSNQTRSGPAVSALCPAVTGVRYG*