AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1020100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1020100 MtrunA17_Chr1g0183691 87.692 65 8 0 56 120 83 147 7.01e-35 123
Msa1020100 MtrunA17_Chr2g0325501 53.333 60 25 1 59 115 98 157 3.42e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1020100 AT1G03900.1 72.581 62 17 0 56 117 85 146 5.29e-26 100
Msa1020100 AT3G58600.1 57.407 54 20 1 59 109 107 160 2.97e-14 69.3

Find 62 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAAAACTTAAGAATTAAT+TGG 0.153202 7_1:-42335600 Msa1020100:three_prime_UTR
CAAGGGAAGCATGTGTTTAT+TGG 0.237220 7_1:-42338085 Msa1020100:CDS
GCCATCCTGCTGTTAATTAT+AGG 0.242602 7_1:-42337956 Msa1020100:CDS
TTTGTAGACCTTCAAGTTAT+AGG 0.264756 7_1:-42335568 Msa1020100:three_prime_UTR
AAGCATGTGTTTATTGGATT+AGG 0.266751 7_1:-42338079 Msa1020100:CDS
TATTTCTTAGTTGGTACATT+TGG 0.267786 7_1:-42337557 Msa1020100:intron
CAAGTTATAGGTTATCCTAT+TGG 0.318712 7_1:-42335556 Msa1020100:three_prime_UTR
ATTTCTTAGTTGGTACATTT+GGG 0.325548 7_1:-42337556 Msa1020100:intron
AGCATGTGTTTATTGGATTA+GGG 0.332772 7_1:-42338078 Msa1020100:CDS
CTTGTCCTATATATATGTTT+TGG 0.337556 7_1:+42338354 None:intergenic
TGCTGTTAATTATAGGCTTA+AGG 0.347777 7_1:-42337949 Msa1020100:intron
TTCTTCCAAAACATATATAT+AGG 0.349435 7_1:-42338359 Msa1020100:CDS
CTTCTAAAGATTGTGTATAA+TGG 0.357548 7_1:-42335808 Msa1020100:intron
TTAAAACTTAAGAATTAATT+GGG 0.366320 7_1:-42335599 Msa1020100:three_prime_UTR
AATACCTATGACTTGCGTTT+TGG 0.377633 7_1:+42336806 None:intergenic
AGAAGCTAATGACCAACTTA+AGG 0.395849 7_1:-42335980 Msa1020100:three_prime_UTR
AGTATTATCAATCATCCAAT+AGG 0.414360 7_1:+42335541 None:intergenic
TGTGAGAAGAGAACATGATA+AGG 0.442504 7_1:-42337996 Msa1020100:CDS
GAAGCTAATGACCAACTTAA+GGG 0.443177 7_1:-42335979 Msa1020100:intron
GCGTTGTGTCCCGCCGTGAC+CGG 0.447886 7_1:-42338243 Msa1020100:CDS
GTGTGAGGGTTCAGTTGAAC+TGG 0.448765 7_1:-42338152 Msa1020100:CDS
TAGGATAACCTATAACTTGA+AGG 0.448867 7_1:+42335560 None:intergenic
ACTGCCAAAACGCAAGTCAT+AGG 0.449501 7_1:-42336810 Msa1020100:intron
TTTCTGCTTAAGATTGAAGA+TGG 0.451141 7_1:-42338109 Msa1020100:CDS
GTTTATTGGATTAGGGTTAA+CGG 0.452324 7_1:-42338071 Msa1020100:CDS
CGCCGGAGGTTGTTCGTGGC+GGG 0.452665 7_1:+42338320 None:intergenic
AAAGGCATGTGTTACGTCAA+TGG 0.457053 7_1:-42335859 Msa1020100:three_prime_UTR
GGTTCCAATCAGACAAGATC+TGG 0.463783 7_1:-42338279 Msa1020100:CDS
AGAGAACATGATAAGGAGTC+TGG 0.464167 7_1:-42337989 Msa1020100:CDS
GGACACAACGCGGAGACGGC+CGG 0.477578 7_1:+42338255 None:intergenic
ATCCCGCCACGAACAACCTC+CGG 0.483393 7_1:-42338322 Msa1020100:CDS
TTAGGCAAATCAACAACTGC+TGG 0.486080 7_1:-42335669 Msa1020100:intron
TAACCGCCGGAGGTTGTTCG+TGG 0.487228 7_1:+42338316 None:intergenic
CGGACCAGATCTTGTCTGAT+TGG 0.492781 7_1:+42338275 None:intergenic
TAAAGTCATTACCCTTAAGT+TGG 0.492910 7_1:+42335968 None:intergenic
TTTCAATTTGAATTGTACTC+TGG 0.494796 7_1:-42335473 Msa1020100:three_prime_UTR
GCGGTTACAAATGCGGCTAA+TGG 0.502661 7_1:-42338300 Msa1020100:CDS
CAATCAGACAAGATCTGGTC+CGG 0.506501 7_1:-42338274 Msa1020100:CDS
GGCGGGACACAACGCGGAGA+CGG 0.509066 7_1:+42338251 None:intergenic
AATTAACAGCAGGATGGCAT+TGG 0.517260 7_1:+42337961 None:intergenic
AGATTTAGCCTGTCGAGTGA+TGG 0.527799 7_1:-42336839 Msa1020100:CDS
ACCGTATCTCACACCGGTCA+CGG 0.531370 7_1:+42338230 None:intergenic
TATCTCACACCGGTCACGGC+GGG 0.540870 7_1:+42338234 None:intergenic
GTAATGTTCCATCACTCGAC+AGG 0.545474 7_1:+42336831 None:intergenic
ACAGGAGTTGAAGTAGAGAA+AGG 0.547321 7_1:-42336028 Msa1020100:intron
CCGCCGGAGGTTGTTCGTGG+CGG 0.571890 7_1:+42338319 None:intergenic
CTGTCTTACATCACATCTCA+AGG 0.573407 7_1:-42337494 Msa1020100:intron
TTAAGCCTATAATTAACAGC+AGG 0.578878 7_1:+42337951 None:intergenic
CTTAAGATTGAAGATGGACA+AGG 0.579822 7_1:-42338103 Msa1020100:CDS
TCAACAACTGCTGGTGTACG+TGG 0.580588 7_1:-42335660 Msa1020100:three_prime_UTR
TTGGATTAGGGTTAACGGAG+AGG 0.594029 7_1:-42338066 Msa1020100:CDS
GCCGCATTTGTAACCGCCGG+AGG 0.612310 7_1:+42338306 None:intergenic
ACCTCCGGCGGTTACAAATG+CGG 0.616884 7_1:-42338307 Msa1020100:CDS
TTAGCCGCATTTGTAACCGC+CGG 0.625611 7_1:+42338303 None:intergenic
GCCTATAATTAACAGCAGGA+TGG 0.636206 7_1:+42337955 None:intergenic
TTCAGCACTGCTAAGACTCG+AGG 0.650870 7_1:-42335628 Msa1020100:three_prime_UTR
GTAAGACAGTTGAAGAAGTG+TGG 0.654301 7_1:+42337508 None:intergenic
TTAAGATTGAAGATGGACAA+GGG 0.676442 7_1:-42338102 Msa1020100:CDS
TGGACAACGACAACACTCGA+CGG 0.686164 7_1:+42337528 None:intergenic
CCGCCACGAACAACCTCCGG+CGG 0.697916 7_1:-42338319 Msa1020100:CDS
GGTCACGGCGGGACACAACG+CGG 0.706808 7_1:+42338245 None:intergenic
GTATCTCACACCGGTCACGG+CGG 0.748302 7_1:+42338233 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATAAATTTACAAAAAAAA+AGG - chr7_1:42335532-42335551 Msa1020100:three_prime_UTR 10.0%
!!! TAAATATATCTTTATTGTTT+TGG - chr7_1:42335962-42335981 Msa1020100:intron 10.0%
!!! AAATATATCTTTATTGTTTT+GGG - chr7_1:42335963-42335982 Msa1020100:intron 10.0%
!! ATTAAAACTTAAGAATTAAT+TGG - chr7_1:42339235-42339254 Msa1020100:intron 10.0%
!! TTAAAACTTAAGAATTAATT+GGG - chr7_1:42339236-42339255 Msa1020100:intron 10.0%
!! TTGAAATGAAAAAAAGATAA+AGG + chr7_1:42335721-42335740 None:intergenic 15.0%
!!! ATCTTTTTTTCATTTCAATT+TGG - chr7_1:42335723-42335742 Msa1020100:intron 15.0%
!!! ACTATTTTTCAACTAAAAAT+TGG + chr7_1:42336975-42336994 None:intergenic 15.0%
!! AACTTAAACTTTGTAAAAAT+AGG - chr7_1:42337068-42337087 Msa1020100:intron 15.0%
!! TACAATTAGAAAATTGAATA+AGG + chr7_1:42337666-42337685 None:intergenic 15.0%
!! TTATAAAAAAATGAGACATA+AGG - chr7_1:42337724-42337743 Msa1020100:intron 15.0%
!! TATAAAAAAATGAGACATAA+GGG - chr7_1:42337725-42337744 Msa1020100:intron 15.0%
!! AGTAAAACTAAAAAATAAGA+TGG + chr7_1:42338663-42338682 None:intergenic 15.0%
!! TTATCTCATAAATTATACAC+AGG + chr7_1:42335512-42335531 None:intergenic 20.0%
!!! AATATTTGTACGATCATTTT+TGG + chr7_1:42335643-42335662 None:intergenic 20.0%
!! ATGATCGTACAAATATTATT+TGG - chr7_1:42335646-42335665 Msa1020100:three_prime_UTR 20.0%
!! GTTACCTAAAATAAAGTTAA+GGG + chr7_1:42335690-42335709 None:intergenic 20.0%
!! TGTTACCTAAAATAAAGTTA+AGG + chr7_1:42335691-42335710 None:intergenic 20.0%
!! CTTGAAAATGTATATGAAAA+TGG + chr7_1:42335759-42335778 None:intergenic 20.0%
!!! TATATATGTTTTGGAAGAAA+AGG + chr7_1:42336475-42336494 Msa1020110:intron 20.0%
!! TTCTTCCAAAACATATATAT+AGG - chr7_1:42336476-42336495 Msa1020100:intron 20.0%
!!! TAATATGATTTGTGAAATGA+AGG - chr7_1:42337691-42337710 Msa1020100:intron 20.0%
!!! AATATGATTTGTGAAATGAA+GGG - chr7_1:42337692-42337711 Msa1020100:intron 20.0%
!!! CTTTATTTCGAATTTGTTAA+TGG - chr7_1:42338681-42338700 Msa1020100:intron 20.0%
!!! AACAATTTTTATGCCATTAA+CGG - chr7_1:42338924-42338943 Msa1020100:intron 20.0%
!!! ATTTTGTACAAAAAAACACA+AGG + chr7_1:42339129-42339148 None:intergenic 20.0%
!!! GTTAGATTTTATGTTTTTGA+TGG - chr7_1:42339327-42339346 Msa1020100:intron 20.0%
!!! CAAGATTCTATTTTAGAAAG+AGG - chr7_1:42335455-42335474 Msa1020100:three_prime_UTR 25.0%
! GAGTTATTTGAACAATCATT+TGG - chr7_1:42335479-42335498 Msa1020100:three_prime_UTR 25.0%
!! TTAGTTTATCTCTACTGATT+TGG + chr7_1:42335576-42335595 None:intergenic 25.0%
!!! TAGTCCCTTAACTTTATTTT+AGG - chr7_1:42335683-42335702 Msa1020100:intron 25.0%
!!! CTTTATTTTAGGTAACAGTT+TGG - chr7_1:42335694-42335713 Msa1020100:intron 25.0%
! GTATATGAAAATGGACAAAA+AGG + chr7_1:42335750-42335769 None:intergenic 25.0%
!!! CCAATTTAATTATTTTGGGA+CGG + chr7_1:42335903-42335922 None:intergenic 25.0%
! CGTCCCAAAATAATTAAATT+GGG - chr7_1:42335901-42335920 Msa1020100:intron 25.0%
!! CGTCCCAATTTAATTATTTT+GGG + chr7_1:42335907-42335926 None:intergenic 25.0%
! CCAAAATAATTAAATTGGGA+CGG - chr7_1:42335905-42335924 Msa1020100:intron 25.0%
!!! ATATCTTTATTGTTTTGGGA+CGG - chr7_1:42335967-42335986 Msa1020100:intron 25.0%
! TGACAAATTTCTTGTAGTTT+GGG + chr7_1:42336038-42336057 None:intergenic 25.0%
! ATGACAAATTTCTTGTAGTT+TGG + chr7_1:42336039-42336058 None:intergenic 25.0%
!! AGATTTAGGTTTCAATTTGA+GGG + chr7_1:42336295-42336314 Msa1020110:exon 25.0%
!! CTTGTCCTATATATATGTTT+TGG + chr7_1:42336484-42336503 Msa1020110:intron 25.0%
!! TATGCATAAAATTTGTTTCC+AGG - chr7_1:42337032-42337051 Msa1020100:intron 25.0%
!! ATGCATAAAATTTGTTTCCA+GGG - chr7_1:42337033-42337052 Msa1020100:intron 25.0%
! TTGTTGTGATATTTCTTAGT+TGG - chr7_1:42337269-42337288 Msa1020100:intron 25.0%
!! TATTTCTTAGTTGGTACATT+TGG - chr7_1:42337278-42337297 Msa1020100:intron 25.0%
!! ATTTCTTAGTTGGTACATTT+GGG - chr7_1:42337279-42337298 Msa1020100:intron 25.0%
! TGTTACTATAGTTGAATACA+AGG - chr7_1:42337384-42337403 Msa1020100:intron 25.0%
! TCTTAAAAAGACAACACAAA+TGG - chr7_1:42337464-42337483 Msa1020100:intron 25.0%
! CTAATATGTGAATAATCAAG+CGG - chr7_1:42337489-42337508 Msa1020100:intron 25.0%
! TTATAATACATACTCTGTGT+CGG + chr7_1:42338143-42338162 None:intergenic 25.0%
!! ACATTTTACTGAAGAAATTC+AGG + chr7_1:42338343-42338362 None:intergenic 25.0%
!!! TATATATGCTTTGTTTTGAC+AGG + chr7_1:42338431-42338450 None:intergenic 25.0%
! CAAAGCATATATATACGTAT+TGG - chr7_1:42338438-42338457 Msa1020100:intron 25.0%
! CTGAAATTAGAGTTCAAAAA+GGG - chr7_1:42338729-42338748 Msa1020100:intron 25.0%
!! CTTCTAAAGATTGTGTATAA+TGG - chr7_1:42339027-42339046 Msa1020100:intron 25.0%
!! TTTTGTACAAAATCAAACCT+TGG - chr7_1:42339136-42339155 Msa1020100:intron 25.0%
! AGTATTATCAATCATCCAAT+AGG + chr7_1:42339297-42339316 None:intergenic 25.0%
!!! TATGTTTTTGATGGAACTAT+TGG - chr7_1:42339336-42339355 Msa1020100:intron 25.0%
! TTTCAATTTGAATTGTACTC+TGG - chr7_1:42339362-42339381 Msa1020100:intron 25.0%
CATGTAAATACAAGACACAA+TGG + chr7_1:42335434-42335453 None:intergenic 30.0%
TTCTTATGCAAACATAGACA+AGG - chr7_1:42335793-42335812 Msa1020100:intron 30.0%
TCTTATGCAAACATAGACAA+GGG - chr7_1:42335794-42335813 Msa1020100:intron 30.0%
! GTTTGATGTTTTTCTTGTAG+TGG + chr7_1:42335850-42335869 None:intergenic 30.0%
AGAAAAACATCAAACTGAGA+CGG - chr7_1:42335855-42335874 Msa1020100:three_prime_UTR 30.0%
CCGTCCCAAAATAATTAAAT+TGG - chr7_1:42335900-42335919 Msa1020100:intron 30.0%
! CCGTCCCAATTTAATTATTT+TGG + chr7_1:42335908-42335927 None:intergenic 30.0%
TTGCAATTCTGTCTCAAAAT+TGG - chr7_1:42335992-42336011 Msa1020100:three_prime_UTR 30.0%
TGCAATTCTGTCTCAAAATT+GGG - chr7_1:42335993-42336012 Msa1020100:three_prime_UTR 30.0%
AAATTTCTTGTAGTTTGGGA+CGG + chr7_1:42336034-42336053 None:intergenic 30.0%
! ACTCTTCACATTAATGCTTT+TGG - chr7_1:42336178-42336197 Msa1020100:intron 30.0%
! CTCTTCACATTAATGCTTTT+GGG - chr7_1:42336179-42336198 Msa1020100:intron 30.0%
TGAGATTGAGGAATAAAATG+GGG + chr7_1:42336268-42336287 None:intergenic 30.0%
GTGAGATTGAGGAATAAAAT+GGG + chr7_1:42336269-42336288 None:intergenic 30.0%
AGTGAGATTGAGGAATAAAA+TGG + chr7_1:42336270-42336289 None:intergenic 30.0%
! GAGATTTAGGTTTCAATTTG+AGG + chr7_1:42336296-42336315 Msa1020110:exon 30.0%
ATAATCCTTCGATTGAACAA+GGG + chr7_1:42336324-42336343 Msa1020110:CDS 30.0%
GCTTAAATGAAACTGGATAA+AGG + chr7_1:42336350-42336369 Msa1020110:CDS 30.0%
!! ACGGAATGGGTTAAAATTAT+GGG + chr7_1:42336378-42336397 Msa1020110:exon 30.0%
!! AACGGAATGGGTTAAAATTA+TGG + chr7_1:42336379-42336398 Msa1020110:exon 30.0%
AGCAACTGTAGAAAAGAAAT+GGG + chr7_1:42336447-42336466 Msa1020110:three_prime_UTR 30.0%
TTTCTGCTTAAGATTGAAGA+TGG - chr7_1:42336726-42336745 Msa1020100:intron 30.0%
TTAAGATTGAAGATGGACAA+GGG - chr7_1:42336733-42336752 Msa1020100:intron 30.0%
AAGCATGTGTTTATTGGATT+AGG - chr7_1:42336756-42336775 Msa1020100:intron 30.0%
AGCATGTGTTTATTGGATTA+GGG - chr7_1:42336757-42336776 Msa1020100:intron 30.0%
! GTTTATTGGATTAGGGTTAA+CGG - chr7_1:42336764-42336783 Msa1020100:intron 30.0%
TTAAGCCTATAATTAACAGC+AGG + chr7_1:42336887-42336906 None:intergenic 30.0%
TGCTGTTAATTATAGGCTTA+AGG - chr7_1:42336886-42336905 Msa1020100:intron 30.0%
! TCTCATGTTTGTGAACTTTT+AGG - chr7_1:42337126-42337145 Msa1020100:intron 30.0%
AGTAACACATGTAAAGAGAA+GGG + chr7_1:42337371-42337390 None:intergenic 30.0%
TAGTAACACATGTAAAGAGA+AGG + chr7_1:42337372-42337391 None:intergenic 30.0%
GTTGCAAGCTATAAACTATA+AGG - chr7_1:42337876-42337895 Msa1020100:intron 30.0%
CAGAAAATTGAATGAACAGA+CGG + chr7_1:42337965-42337984 None:intergenic 30.0%
CAATTTCTTCTGTCAATATC+TGG - chr7_1:42338235-42338254 Msa1020100:intron 30.0%
AACTAATGAAGTTACCTCTT+GGG + chr7_1:42338606-42338625 None:intergenic 30.0%
AAACTAATGAAGTTACCTCT+TGG + chr7_1:42338607-42338626 None:intergenic 30.0%
AAGAGGTAACTTCATTAGTT+TGG - chr7_1:42338606-42338625 Msa1020100:intron 30.0%
GCTGAAATTAGAGTTCAAAA+AGG - chr7_1:42338728-42338747 Msa1020100:intron 30.0%
TAAAGTCATTACCCTTAAGT+TGG + chr7_1:42338870-42338889 None:intergenic 30.0%
TCTGCTTTGATAGAGAAAAA+AGG - chr7_1:42338958-42338977 Msa1020100:intron 30.0%
TCAAACCTTGGATATTTCTT+AGG - chr7_1:42339148-42339167 Msa1020100:intron 30.0%
ATTTGCCTAAGAAATATCCA+AGG + chr7_1:42339156-42339175 None:intergenic 30.0%
! TTTGTAGACCTTCAAGTTAT+AGG - chr7_1:42339267-42339286 Msa1020100:intron 30.0%
TAGGATAACCTATAACTTGA+AGG + chr7_1:42339278-42339297 None:intergenic 30.0%
CAAGTTATAGGTTATCCTAT+TGG - chr7_1:42339279-42339298 Msa1020100:intron 30.0%
GTAAATACAAGACACAATGG+AGG + chr7_1:42335431-42335450 None:intergenic 35.0%
ATCAAACTGAGACGGAATTT+CGG - chr7_1:42335863-42335882 Msa1020100:three_prime_UTR 35.0%
ATTCTGTCTCAAAATTGGGA+CGG - chr7_1:42335997-42336016 Msa1020100:three_prime_UTR 35.0%
!!! TTTCATGGTTTTGGAGTTGT+GGG + chr7_1:42336245-42336264 None:intergenic 35.0%
! AAAATGGGGTTTCATGGTTT+TGG + chr7_1:42336254-42336273 None:intergenic 35.0%
AGGAATAAAATGGGGTTTCA+TGG + chr7_1:42336260-42336279 None:intergenic 35.0%
CATAATCCTTCGATTGAACA+AGG + chr7_1:42336325-42336344 Msa1020110:CDS 35.0%
AGAAAAGAAATGGGTCGAAA+AGG + chr7_1:42336438-42336457 Msa1020110:three_prime_UTR 35.0%
GAGCAACTGTAGAAAAGAAA+TGG + chr7_1:42336448-42336467 Msa1020110:three_prime_UTR 35.0%
CTTAAGATTGAAGATGGACA+AGG - chr7_1:42336732-42336751 Msa1020100:intron 35.0%
TGTGAGAAGAGAACATGATA+AGG - chr7_1:42336839-42336858 Msa1020100:CDS 35.0%
! TAGACATTGGTTAGACACAT+GGG + chr7_1:42337101-42337120 None:intergenic 35.0%
! ATAGACATTGGTTAGACACA+TGG + chr7_1:42337102-42337121 None:intergenic 35.0%
ACATGAGACAGTATAGACAT+TGG + chr7_1:42337114-42337133 None:intergenic 35.0%
TATAGTTGAATACAAGGTCG+TGG - chr7_1:42337390-42337409 Msa1020100:intron 35.0%
AGACATAAGGGCTAAAAGAA+AGG - chr7_1:42337737-42337756 Msa1020100:intron 35.0%
TGATATGCATGTTAAGAACG+AGG + chr7_1:42337787-42337806 None:intergenic 35.0%
CAACACATATATCTTTCCTC+AGG + chr7_1:42337849-42337868 None:intergenic 35.0%
!! AATACCTATGACTTGCGTTT+TGG + chr7_1:42338032-42338051 None:intergenic 35.0%
TCATATGCACATTCAGAAAG+AGG + chr7_1:42338072-42338091 None:intergenic 35.0%
ATTGACACACATTCGTAAAG+AGG + chr7_1:42338219-42338238 None:intergenic 35.0%
TTCTTCTGTCAATATCTGGA+TGG - chr7_1:42338239-42338258 Msa1020100:intron 35.0%
! GTCTTTTCTGATATGTTCTG+CGG - chr7_1:42338261-42338280 Msa1020100:CDS 35.0%
!!! TTTTAGAACCTACGCTTCAA+TGG + chr7_1:42338313-42338332 None:intergenic 35.0%
AGCTCTCTTGAAATAGTGTT+AGG - chr7_1:42338578-42338597 Msa1020100:intron 35.0%
! AGGTAACTTCATTAGTTTGG+TGG - chr7_1:42338609-42338628 Msa1020100:intron 35.0%
AAAAGGGCTATGATATCATC+AGG - chr7_1:42338745-42338764 Msa1020100:intron 35.0%
AAAGGGCTATGATATCATCA+GGG - chr7_1:42338746-42338765 Msa1020100:intron 35.0%
AGAAGCTAATGACCAACTTA+AGG - chr7_1:42338855-42338874 Msa1020100:intron 35.0%
GAAGCTAATGACCAACTTAA+GGG - chr7_1:42338856-42338875 Msa1020100:intron 35.0%
CTTAAACATGTGTACACCAA+AGG + chr7_1:42339095-42339114 None:intergenic 35.0%
AAGACACAATGGAGGATTGT+TGG + chr7_1:42335423-42335442 None:intergenic 40.0%
TCAAACTGAGACGGAATTTC+GGG - chr7_1:42335864-42335883 Msa1020100:three_prime_UTR 40.0%
! CTGCAACTGTTCTAAGCTAA+GGG + chr7_1:42336082-42336101 None:intergenic 40.0%
! TCTGCAACTGTTCTAAGCTA+AGG + chr7_1:42336083-42336102 None:intergenic 40.0%
! TTTTGCAACAGTTGCTACGA+CGG - chr7_1:42336109-42336128 Msa1020100:intron 40.0%
!!! GTTTCATGGTTTTGGAGTTG+TGG + chr7_1:42336246-42336265 None:intergenic 40.0%
TTTGAGGGAAAGTGAGATTG+AGG + chr7_1:42336280-42336299 Msa1020110:exon 40.0%
AACAAGGGTGGTAGAGATTT+AGG + chr7_1:42336309-42336328 Msa1020110:CDS 40.0%
GGTGAGAGCTTAAATGAAAC+TGG + chr7_1:42336357-42336376 Msa1020110:CDS 40.0%
AGTGAAGAACTGTGGAGAAA+CGG + chr7_1:42336397-42336416 Msa1020110:three_prime_UTR 40.0%
CAACAAAGAGTGAAGAACTG+TGG + chr7_1:42336405-42336424 Msa1020110:three_prime_UTR 40.0%
! CAAGGGAAGCATGTGTTTAT+TGG - chr7_1:42336750-42336769 Msa1020100:intron 40.0%
AGAGAACATGATAAGGAGTC+TGG - chr7_1:42336846-42336865 Msa1020100:intron 40.0%
AATTAACAGCAGGATGGCAT+TGG + chr7_1:42336877-42336896 None:intergenic 40.0%
GCCATCCTGCTGTTAATTAT+AGG - chr7_1:42336879-42336898 Msa1020100:intron 40.0%
GCCTATAATTAACAGCAGGA+TGG + chr7_1:42336883-42336902 None:intergenic 40.0%
!! TTTGTTTCCAGGGTACTGTT+AGG - chr7_1:42337043-42337062 Msa1020100:intron 40.0%
GTAAGACAGTTGAAGAAGTG+TGG + chr7_1:42337330-42337349 None:intergenic 40.0%
CTGTCTTACATCACATCTCA+AGG - chr7_1:42337341-42337360 Msa1020100:intron 40.0%
TTTCAACCAAATGAGCTCAC+AGG + chr7_1:42337425-42337444 None:intergenic 40.0%
CTCATGAAAAAACAGACCAG+AGG + chr7_1:42337527-42337546 None:intergenic 40.0%
ATTCCTTCTAATGCAACCTG+AGG - chr7_1:42337830-42337849 Msa1020100:intron 40.0%
! TTTCCTCAGGTTGCATTAGA+AGG + chr7_1:42337836-42337855 None:intergenic 40.0%
CTCTGATTATCTTTAGCGAC+AGG - chr7_1:42338108-42338127 Msa1020100:CDS 40.0%
TTGCAAAACCATTGAAGCGT+AGG - chr7_1:42338302-42338321 Msa1020100:CDS 40.0%
TGCTATGCTCATTCAGATAG+AGG + chr7_1:42338368-42338387 None:intergenic 40.0%
AATAGTGTTAGGAACCCAAG+AGG - chr7_1:42338589-42338608 Msa1020100:intron 40.0%
TGATATCATCAGGGTGAGTT+TGG - chr7_1:42338755-42338774 Msa1020100:intron 40.0%
! CTAGTTGTTCTTTTGTCGAC+AGG - chr7_1:42338789-42338808 Msa1020100:intron 40.0%
ACAGGAGTTGAAGTAGAGAA+AGG - chr7_1:42338807-42338826 Msa1020100:intron 40.0%
GATGATGCTTTCACCGTTAA+TGG + chr7_1:42338940-42338959 None:intergenic 40.0%
! AAAGGCATGTGTTACGTCAA+TGG - chr7_1:42338976-42338995 Msa1020100:intron 40.0%
TGATAGCTTGACATTGCCTT+TGG - chr7_1:42339076-42339095 Msa1020100:intron 40.0%
TTAGGCAAATCAACAACTGC+TGG - chr7_1:42339166-42339185 Msa1020100:intron 40.0%
CGGAATTTGCGACGCAATTA+TGG - chr7_1:42335925-42335944 Msa1020100:intron 45.0%
TGCTACGACGGTTAGACATA+AGG - chr7_1:42336121-42336140 Msa1020100:intron 45.0%
GCTACGACGGTTAGACATAA+GGG - chr7_1:42336122-42336141 Msa1020100:intron 45.0%
TGTGGGAACGAGACTGAAAT+TGG + chr7_1:42336228-42336247 None:intergenic 45.0%
TACCACCCTTGTTCAATCGA+AGG - chr7_1:42336316-42336335 Msa1020100:intron 45.0%
ATCCTTCGATTGAACAAGGG+TGG + chr7_1:42336321-42336340 Msa1020110:CDS 45.0%
GAACTGTGGAGAAACGGAAT+GGG + chr7_1:42336391-42336410 Msa1020110:three_prime_UTR 45.0%
AGAACTGTGGAGAAACGGAA+TGG + chr7_1:42336392-42336411 Msa1020110:three_prime_UTR 45.0%
GGTTCCAATCAGACAAGATC+TGG - chr7_1:42336556-42336575 Msa1020100:intron 45.0%
CAATCAGACAAGATCTGGTC+CGG - chr7_1:42336561-42336580 Msa1020100:intron 45.0%
CTTCTAACCGTATCTCACAC+CGG + chr7_1:42336614-42336633 Msa1020110:three_prime_UTR 45.0%
CGGTTAGAAGATCCCAACTT+CGG - chr7_1:42336624-42336643 Msa1020100:intron 45.0%
! TGTTTTTCCCTGTCAGTGTG+AGG - chr7_1:42336668-42336687 Msa1020100:intron 45.0%
! GTTTTTCCCTGTCAGTGTGA+GGG - chr7_1:42336669-42336688 Msa1020100:intron 45.0%
! TTGGATTAGGGTTAACGGAG+AGG - chr7_1:42336769-42336788 Msa1020100:intron 45.0%
AAGTTTGCCTAACAGTACCC+TGG + chr7_1:42337053-42337072 None:intergenic 45.0%
TGAATACAAGGTCGTGGCAT+TGG - chr7_1:42337396-42337415 Msa1020100:intron 45.0%
AGATTTAGCCTGTCGAGTGA+TGG - chr7_1:42337996-42338015 Msa1020100:CDS 45.0%
GTAATGTTCCATCACTCGAC+AGG + chr7_1:42338007-42338026 None:intergenic 45.0%
ACTGCCAAAACGCAAGTCAT+AGG - chr7_1:42338025-42338044 Msa1020100:CDS 45.0%
! CATACTCTGTGTCGGTACAA+AGG + chr7_1:42338135-42338154 None:intergenic 45.0%
!!! ACTTTAAAATAATATTTTAT+TGG - chr7_1:42337575-42337594 Msa1020100:intron 5.0%
!!! TTTTTAACAATATTATATTT+TGG + chr7_1:42338487-42338506 None:intergenic 5.0%
ACTGAGACGGAATTTCGGGA+CGG - chr7_1:42335868-42335887 Msa1020100:intron 50.0%
! GTTCTAAGCTAAGGGTCGCA+AGG + chr7_1:42336074-42336093 None:intergenic 50.0%
TTAGCCGCATTTGTAACCGC+CGG + chr7_1:42336535-42336554 Msa1020110:intron 50.0%
GCGGTTACAAATGCGGCTAA+TGG - chr7_1:42336535-42336554 Msa1020100:intron 50.0%
CGGACCAGATCTTGTCTGAT+TGG + chr7_1:42336563-42336582 Msa1020110:intron 50.0%
ACTGAACCCTCACACTGACA+GGG + chr7_1:42336678-42336697 None:intergenic 50.0%
AACTGAACCCTCACACTGAC+AGG + chr7_1:42336679-42336698 None:intergenic 50.0%
GTGTGAGGGTTCAGTTGAAC+TGG - chr7_1:42336683-42336702 Msa1020100:intron 50.0%
TGGACAACGACAACACTCGA+CGG + chr7_1:42337310-42337329 None:intergenic 50.0%
TGGTGACCTGTGAGCTCATT+TGG - chr7_1:42337416-42337435 Msa1020100:intron 50.0%
GCGGCATTGCATATTACCTC+TGG - chr7_1:42337508-42337527 Msa1020100:CDS 50.0%
GACGGACATGCATGTGCAAA+AGG + chr7_1:42337947-42337966 None:intergenic 50.0%
TCAACAACTGCTGGTGTACG+TGG - chr7_1:42339175-42339194 Msa1020100:intron 50.0%
TTCAGCACTGCTAAGACTCG+AGG - chr7_1:42339207-42339226 Msa1020100:intron 50.0%
CGGTTAGACATAAGGGTCGC+AGG - chr7_1:42336129-42336148 Msa1020100:intron 55.0%
ACCTCCGGCGGTTACAAATG+CGG - chr7_1:42336528-42336547 Msa1020100:intron 55.0%
ACCGTATCTCACACCGGTCA+CGG + chr7_1:42336608-42336627 Msa1020110:three_prime_UTR 55.0%
GCGAAGAGATCGCCGAAGTT+GGG + chr7_1:42336639-42336658 None:intergenic 55.0%
AGCGAAGAGATCGCCGAAGT+TGG + chr7_1:42336640-42336659 None:intergenic 55.0%
ATCCCGCCACGAACAACCTC+CGG - chr7_1:42336513-42336532 Msa1020100:intron 60.0%
TAACCGCCGGAGGTTGTTCG+TGG + chr7_1:42336522-42336541 Msa1020110:intron 60.0%
TATCTCACACCGGTCACGGC+GGG + chr7_1:42336604-42336623 Msa1020110:three_prime_UTR 60.0%
GTATCTCACACCGGTCACGG+CGG + chr7_1:42336605-42336624 Msa1020110:three_prime_UTR 60.0%
GCCGTGACCGGTGTGAGATA+CGG - chr7_1:42336604-42336623 Msa1020100:intron 60.0%
GCCGCATTTGTAACCGCCGG+AGG + chr7_1:42336532-42336551 Msa1020110:intron 65.0%
CGCCGGAGGTTGTTCGTGGC+GGG + chr7_1:42336518-42336537 Msa1020110:intron 70.0%
CCGCCGGAGGTTGTTCGTGG+CGG + chr7_1:42336519-42336538 Msa1020110:intron 70.0%
CCGCCACGAACAACCTCCGG+CGG - chr7_1:42336516-42336535 Msa1020100:intron 70.0%
GGACACAACGCGGAGACGGC+CGG + chr7_1:42336583-42336602 Msa1020110:three_prime_UTR 70.0%
GGCGGGACACAACGCGGAGA+CGG + chr7_1:42336587-42336606 Msa1020110:three_prime_UTR 70.0%
GGTCACGGCGGGACACAACG+CGG + chr7_1:42336593-42336612 Msa1020110:three_prime_UTR 70.0%
GCGTTGTGTCCCGCCGTGAC+CGG - chr7_1:42336592-42336611 Msa1020100:intron 70.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 42335406 42339451 42335406 ID=Msa1020100;Name=Msa1020100
chr7_1 mRNA 42335406 42339451 42335406 ID=Msa1020100-mRNA-1;Parent=Msa1020100;Name=Msa1020100-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0|0.71|0.62|0.87|0.28|0.37|8|427|169
chr7_1 exon 42335406 42335687 42335406 ID=Msa1020100-mRNA-1:exon:8349;Parent=Msa1020100-mRNA-1
chr7_1 exon 42335809 42335886 42335809 ID=Msa1020100-mRNA-1:exon:8348;Parent=Msa1020100-mRNA-1
chr7_1 exon 42335980 42336046 42335980 ID=Msa1020100-mRNA-1:exon:8347;Parent=Msa1020100-mRNA-1
chr7_1 exon 42336811 42336859 42336811 ID=Msa1020100-mRNA-1:exon:8346;Parent=Msa1020100-mRNA-1
chr7_1 exon 42337495 42337569 42337495 ID=Msa1020100-mRNA-1:exon:8345;Parent=Msa1020100-mRNA-1
chr7_1 exon 42337950 42338173 42337950 ID=Msa1020100-mRNA-1:exon:8344;Parent=Msa1020100-mRNA-1
chr7_1 exon 42338242 42338395 42338242 ID=Msa1020100-mRNA-1:exon:8343;Parent=Msa1020100-mRNA-1
chr7_1 exon 42339444 42339451 42339444 ID=Msa1020100-mRNA-1:exon:8342;Parent=Msa1020100-mRNA-1
chr7_1 CDS 42339444 42339451 42339444 ID=Msa1020100-mRNA-1:cds;Parent=Msa1020100-mRNA-1
chr7_1 CDS 42338242 42338395 42338242 ID=Msa1020100-mRNA-1:cds;Parent=Msa1020100-mRNA-1
chr7_1 CDS 42337950 42338173 42337950 ID=Msa1020100-mRNA-1:cds;Parent=Msa1020100-mRNA-1
chr7_1 CDS 42337495 42337569 42337495 ID=Msa1020100-mRNA-1:cds;Parent=Msa1020100-mRNA-1
chr7_1 CDS 42336811 42336859 42336811 ID=Msa1020100-mRNA-1:cds;Parent=Msa1020100-mRNA-1
chr7_1 three_prime_UTR 42335980 42336046 42335980 ID=Msa1020100-mRNA-1:three_prime_utr;Parent=Msa1020100-mRNA-1
chr7_1 three_prime_UTR 42335809 42335886 42335809 ID=Msa1020100-mRNA-1:three_prime_utr;Parent=Msa1020100-mRNA-1
chr7_1 three_prime_UTR 42335406 42335687 42335406 ID=Msa1020100-mRNA-1:three_prime_utr;Parent=Msa1020100-mRNA-1
Gene Sequence

>Msa1020100

ATGTGGTCTTGCTCAGTTCCTTTTCTTCCAAAACATATATATAGGACAAGATCTACAAGATCCCGCCACGAACAACCTCCGGCGGTTACAAATGCGGCTAATGGTTCCAATCAGACAAGATCTGGTCCGGCCGTCTCCGCGTTGTGTCCCGCCGTGACCGGTTGTGAGGGTTCAGTTGAACTGGTTGTCGATTCATCGAGATACTTTCTGCTTAAGATTGAAGATGGACAAGGGAAGCATGTGTTTATTGGATTAGGGTTAACGGAGAGGAGTGAAGCTTTTGATTTCAATGTTGCGCTTTCTGATCATGAGAAGTATGTGAGAAGAGAACATGATAAGGAGTCTGGTGATGCCAATGCCATCCTGCTGTTAATTATAGGCTTAAGTTGGTACATTTGGGATTTGCCGTCGAGTGTTGTCGTTGTCCACACTTCTTCAACTGTCTTACATCACATCTCAAGATTTAGCCTGTCGAGTGATGGAACATTACTGCCAAAACGCAAGTCATAG
Protein sequence

>Msa1020100

MWSCSVPFLPKHIYRTRSTRSRHEQPPAVTNAANGSNQTRSGPAVSALCPAVTGCEGSVELVVDSSRYFLLKIEDGQGKHVFIGLGLTERSEAFDFNVALSDHEKYVRREHDKESGDANAILLLIIGLSWYIWDLPSSVVVVHTSSTVLHHISRFSLSSDGTLLPKRKS*