AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1320580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 95 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAAAACTTAAGAATTAAT+TGG 0.153202 8_4:+25214135 Msa1320580:three_prime_UTR
AACGGAATGGGTTAAAATTA+TGG 0.195683 8_4:-25211276 None:intergenic
ACTCTTCACATTAATGCTTT+TGG 0.207367 8_4:+25211078 None:intergenic
AAAATGGGGTTTCATGGTTT+TGG 0.235392 8_4:-25211151 None:intergenic
AACAAGGGTGGTAGAGATTT+AGG 0.237128 8_4:-25211206 None:intergenic
CAAGGGAAGCATGTGTTTAT+TGG 0.237220 8_4:+25211650 Msa1320580:three_prime_UTR
GCCATCCTGCTGTTAATTAT+AGG 0.242602 8_4:+25211779 Msa1320580:three_prime_UTR
TTTGTAGACCTTCAAGTTAT+AGG 0.264756 8_4:+25214167 Msa1320580:three_prime_UTR
AAGCATGTGTTTATTGGATT+AGG 0.266751 8_4:+25211656 Msa1320580:three_prime_UTR
TATTTCTTAGTTGGTACATT+TGG 0.267786 8_4:+25212178 Msa1320580:intron
AGTGAGATTGAGGAATAAAA+TGG 0.312064 8_4:-25211167 None:intergenic
AGGAATAAAATGGGGTTTCA+TGG 0.315510 8_4:-25211157 None:intergenic
CAAGTTATAGGTTATCCTAT+TGG 0.318712 8_4:+25214179 Msa1320580:three_prime_UTR
ATTTCTTAGTTGGTACATTT+GGG 0.325548 8_4:+25212179 Msa1320580:intron
AGCATGTGTTTATTGGATTA+GGG 0.332772 8_4:+25211657 Msa1320580:three_prime_UTR
CTTGTCCTATATATATGTTT+TGG 0.337556 8_4:-25211381 None:intergenic
TGCTGTTAATTATAGGCTTA+AGG 0.347777 8_4:+25211786 Msa1320580:three_prime_UTR
TTCTTCCAAAACATATATAT+AGG 0.349435 8_4:+25211376 Msa1320580:CDS
CTTCTAAAGATTGTGTATAA+TGG 0.357548 8_4:+25213927 Msa1320580:three_prime_UTR
GAGCAACTGTAGAAAAGAAA+TGG 0.357579 8_4:-25211345 None:intergenic
TTAAAACTTAAGAATTAATT+GGG 0.366320 8_4:+25214136 Msa1320580:three_prime_UTR
TGTGGGAACGAGACTGAAAT+TGG 0.369214 8_4:-25211125 None:intergenic
GGTGAGAGCTTAAATGAAAC+TGG 0.376751 8_4:-25211254 None:intergenic
AATACCTATGACTTGCGTTT+TGG 0.377633 8_4:-25212929 None:intergenic
GAGATTTAGGTTTCAATTTG+AGG 0.394504 8_4:-25211193 None:intergenic
AGAAGCTAATGACCAACTTA+AGG 0.395849 8_4:+25213755 Msa1320580:three_prime_UTR
ACGGAATGGGTTAAAATTAT+GGG 0.402922 8_4:-25211275 None:intergenic
AGTATTATCAATCATCCAAT+AGG 0.414360 8_4:-25214194 None:intergenic
AACTGAACCCTCACACTGAC+AGG 0.423043 8_4:-25211576 None:intergenic
GTGAGATTGAGGAATAAAAT+GGG 0.426659 8_4:-25211166 None:intergenic
TGTGAGAAGAGAACATGATA+AGG 0.442504 8_4:+25211739 Msa1320580:three_prime_UTR
GAAGCTAATGACCAACTTAA+GGG 0.443177 8_4:+25213756 Msa1320580:three_prime_UTR
GCTTAAATGAAACTGGATAA+AGG 0.443636 8_4:-25211247 None:intergenic
GCGTTGTGTCCCGCCGTGAC+CGG 0.447886 8_4:+25211492 Msa1320580:three_prime_UTR
GTGTGAGGGTTCAGTTGAAC+TGG 0.448765 8_4:+25211583 Msa1320580:three_prime_UTR
TAGGATAACCTATAACTTGA+AGG 0.448867 8_4:-25214175 None:intergenic
ACTGCCAAAACGCAAGTCAT+AGG 0.449501 8_4:+25212925 Msa1320580:three_prime_UTR
AGATTTAGGTTTCAATTTGA+GGG 0.451063 8_4:-25211192 None:intergenic
TTTCTGCTTAAGATTGAAGA+TGG 0.451141 8_4:+25211626 Msa1320580:three_prime_UTR
GTTTATTGGATTAGGGTTAA+CGG 0.452324 8_4:+25211664 Msa1320580:three_prime_UTR
CGCCGGAGGTTGTTCGTGGC+GGG 0.452665 8_4:-25211415 None:intergenic
AAAGGCATGTGTTACGTCAA+TGG 0.457053 8_4:+25213876 Msa1320580:three_prime_UTR
AGCGAAGAGATCGCCGAAGT+TGG 0.459540 8_4:-25211537 None:intergenic
GGTTCCAATCAGACAAGATC+TGG 0.463783 8_4:+25211456 Msa1320580:three_prime_UTR
AGAGAACATGATAAGGAGTC+TGG 0.464167 8_4:+25211746 Msa1320580:three_prime_UTR
GCGAAGAGATCGCCGAAGTT+GGG 0.467204 8_4:-25211536 None:intergenic
GCCGTGACCGGTGTGAGATA+CGG 0.467324 8_4:+25211504 Msa1320580:three_prime_UTR
GGACACAACGCGGAGACGGC+CGG 0.477578 8_4:-25211480 None:intergenic
TTTGAGGGAAAGTGAGATTG+AGG 0.478972 8_4:-25211177 None:intergenic
ATCCCGCCACGAACAACCTC+CGG 0.483393 8_4:+25211413 Msa1320580:three_prime_UTR
TTAGGCAAATCAACAACTGC+TGG 0.486080 8_4:+25214066 Msa1320580:intron
TAACCGCCGGAGGTTGTTCG+TGG 0.487228 8_4:-25211419 None:intergenic
CGGACCAGATCTTGTCTGAT+TGG 0.492781 8_4:-25211460 None:intergenic
TAAAGTCATTACCCTTAAGT+TGG 0.492910 8_4:-25213767 None:intergenic
AGTGAAGAACTGTGGAGAAA+CGG 0.493197 8_4:-25211294 None:intergenic
TTTCAATTTGAATTGTACTC+TGG 0.494796 8_4:+25214262 Msa1320580:three_prime_UTR
GCGGTTACAAATGCGGCTAA+TGG 0.502661 8_4:+25211435 Msa1320580:three_prime_UTR
AGAAAAGAAATGGGTCGAAA+AGG 0.503425 8_4:-25211335 None:intergenic
CAATCAGACAAGATCTGGTC+CGG 0.506501 8_4:+25211461 Msa1320580:three_prime_UTR
GGCGGGACACAACGCGGAGA+CGG 0.509066 8_4:-25211484 None:intergenic
AGCAACTGTAGAAAAGAAAT+GGG 0.514548 8_4:-25211344 None:intergenic
AATTAACAGCAGGATGGCAT+TGG 0.517260 8_4:-25211774 None:intergenic
AGATTTAGCCTGTCGAGTGA+TGG 0.527799 8_4:+25212896 Msa1320580:intron
ACCGTATCTCACACCGGTCA+CGG 0.531370 8_4:-25211505 None:intergenic
CATAATCCTTCGATTGAACA+AGG 0.536572 8_4:-25211222 None:intergenic
TATCTCACACCGGTCACGGC+GGG 0.540870 8_4:-25211501 None:intergenic
GTAATGTTCCATCACTCGAC+AGG 0.545474 8_4:-25212904 None:intergenic
ACAGGAGTTGAAGTAGAGAA+AGG 0.547321 8_4:+25213707 Msa1320580:intron
TACCACCCTTGTTCAATCGA+AGG 0.561434 8_4:+25211216 Msa1320580:five_prime_UTR
CCGCCGGAGGTTGTTCGTGG+CGG 0.571890 8_4:-25211416 None:intergenic
CTGTCTTACATCACATCTCA+AGG 0.573407 8_4:+25212241 Msa1320580:three_prime_UTR
TTAAGCCTATAATTAACAGC+AGG 0.578878 8_4:-25211784 None:intergenic
CTTAAGATTGAAGATGGACA+AGG 0.579822 8_4:+25211632 Msa1320580:three_prime_UTR
TCAACAACTGCTGGTGTACG+TGG 0.580588 8_4:+25214075 Msa1320580:three_prime_UTR
GAACTGTGGAGAAACGGAAT+GGG 0.584247 8_4:-25211288 None:intergenic
AGAACTGTGGAGAAACGGAA+TGG 0.592306 8_4:-25211289 None:intergenic
TTGGATTAGGGTTAACGGAG+AGG 0.594029 8_4:+25211669 Msa1320580:three_prime_UTR
TGAGATTGAGGAATAAAATG+GGG 0.611914 8_4:-25211165 None:intergenic
GCCGCATTTGTAACCGCCGG+AGG 0.612310 8_4:-25211429 None:intergenic
ACCTCCGGCGGTTACAAATG+CGG 0.616884 8_4:+25211428 Msa1320580:three_prime_UTR
ACTGAACCCTCACACTGACA+GGG 0.621101 8_4:-25211575 None:intergenic
TTAGCCGCATTTGTAACCGC+CGG 0.625611 8_4:-25211432 None:intergenic
GCCTATAATTAACAGCAGGA+TGG 0.636206 8_4:-25211780 None:intergenic
ATCCTTCGATTGAACAAGGG+TGG 0.639676 8_4:-25211218 None:intergenic
CGGTTAGAAGATCCCAACTT+CGG 0.643782 8_4:+25211524 Msa1320580:three_prime_UTR
CAACAAAGAGTGAAGAACTG+TGG 0.649585 8_4:-25211302 None:intergenic
TTCAGCACTGCTAAGACTCG+AGG 0.650870 8_4:+25214107 Msa1320580:three_prime_UTR
GTAAGACAGTTGAAGAAGTG+TGG 0.654301 8_4:-25212227 None:intergenic
TTAAGATTGAAGATGGACAA+GGG 0.676442 8_4:+25211633 Msa1320580:three_prime_UTR
TGGACAACGACAACACTCGA+CGG 0.686164 8_4:-25212207 None:intergenic
CTTCTAACCGTATCTCACAC+CGG 0.687609 8_4:-25211511 None:intergenic
CCGCCACGAACAACCTCCGG+CGG 0.697916 8_4:+25211416 Msa1320580:three_prime_UTR
GGTCACGGCGGGACACAACG+CGG 0.706808 8_4:-25211490 None:intergenic
ATAATCCTTCGATTGAACAA+GGG 0.716702 8_4:-25211221 None:intergenic
GTATCTCACACCGGTCACGG+CGG 0.748302 8_4:-25211502 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAACTTAAGAATTAAT+TGG + chr8_4:25214135-25214154 Msa1320580:three_prime_UTR 10.0%
!! TTAAAACTTAAGAATTAATT+GGG + chr8_4:25214136-25214155 Msa1320580:three_prime_UTR 10.0%
!!! ACTATTTTTCAACTAAAAAT+TGG - chr8_4:25211875-25211894 None:intergenic 15.0%
!! AACTTAAACTTTGTAAAAAT+AGG + chr8_4:25211968-25211987 Msa1320580:intron 15.0%
!! TACAATTAGAAAATTGAATA+AGG - chr8_4:25212566-25212585 None:intergenic 15.0%
!! TTATAAAAAAATGAGACATA+AGG + chr8_4:25212624-25212643 Msa1320580:intron 15.0%
!! TATAAAAAAATGAGACATAA+GGG + chr8_4:25212625-25212644 Msa1320580:intron 15.0%
!! AGTAAAACTAAAAAATAAGA+TGG - chr8_4:25213563-25213582 None:intergenic 15.0%
!!! TATATATGTTTTGGAAGAAA+AGG - chr8_4:25211375-25211394 None:intergenic 20.0%
!! TTCTTCCAAAACATATATAT+AGG + chr8_4:25211376-25211395 Msa1320580:CDS 20.0%
!!! TAATATGATTTGTGAAATGA+AGG + chr8_4:25212591-25212610 Msa1320580:intron 20.0%
!!! AATATGATTTGTGAAATGAA+GGG + chr8_4:25212592-25212611 Msa1320580:intron 20.0%
!!! CTTTATTTCGAATTTGTTAA+TGG + chr8_4:25213581-25213600 Msa1320580:intron 20.0%
!!! AACAATTTTTATGCCATTAA+CGG + chr8_4:25213824-25213843 Msa1320580:intron 20.0%
!!! ATTTTGTACAAAAAAACACA+AGG - chr8_4:25214029-25214048 None:intergenic 20.0%
!!! GTTAGATTTTATGTTTTTGA+TGG + chr8_4:25214227-25214246 Msa1320580:three_prime_UTR 20.0%
!! AGATTTAGGTTTCAATTTGA+GGG - chr8_4:25211195-25211214 None:intergenic 25.0%
!! CTTGTCCTATATATATGTTT+TGG - chr8_4:25211384-25211403 None:intergenic 25.0%
!! TATGCATAAAATTTGTTTCC+AGG + chr8_4:25211932-25211951 Msa1320580:intron 25.0%
!! ATGCATAAAATTTGTTTCCA+GGG + chr8_4:25211933-25211952 Msa1320580:intron 25.0%
! TTGTTGTGATATTTCTTAGT+TGG + chr8_4:25212169-25212188 Msa1320580:intron 25.0%
!! TATTTCTTAGTTGGTACATT+TGG + chr8_4:25212178-25212197 Msa1320580:intron 25.0%
!! ATTTCTTAGTTGGTACATTT+GGG + chr8_4:25212179-25212198 Msa1320580:intron 25.0%
! TGTTACTATAGTTGAATACA+AGG + chr8_4:25212284-25212303 Msa1320580:intron 25.0%
! TCTTAAAAAGACAACACAAA+TGG + chr8_4:25212364-25212383 Msa1320580:intron 25.0%
! CTAATATGTGAATAATCAAG+CGG + chr8_4:25212389-25212408 Msa1320580:intron 25.0%
! TTATAATACATACTCTGTGT+CGG - chr8_4:25213043-25213062 None:intergenic 25.0%
!! ACATTTTACTGAAGAAATTC+AGG - chr8_4:25213243-25213262 None:intergenic 25.0%
!!! TATATATGCTTTGTTTTGAC+AGG - chr8_4:25213331-25213350 None:intergenic 25.0%
! CAAAGCATATATATACGTAT+TGG + chr8_4:25213338-25213357 Msa1320580:intron 25.0%
! CTGAAATTAGAGTTCAAAAA+GGG + chr8_4:25213629-25213648 Msa1320580:intron 25.0%
!! CTTCTAAAGATTGTGTATAA+TGG + chr8_4:25213927-25213946 Msa1320580:three_prime_UTR 25.0%
!! TTTTGTACAAAATCAAACCT+TGG + chr8_4:25214036-25214055 Msa1320580:intron 25.0%
! AGTATTATCAATCATCCAAT+AGG - chr8_4:25214197-25214216 None:intergenic 25.0%
!!! TATGTTTTTGATGGAACTAT+TGG + chr8_4:25214236-25214255 Msa1320580:three_prime_UTR 25.0%
! TTTCAATTTGAATTGTACTC+TGG + chr8_4:25214262-25214281 Msa1320580:three_prime_UTR 25.0%
TGAGATTGAGGAATAAAATG+GGG - chr8_4:25211168-25211187 None:intergenic 30.0%
GTGAGATTGAGGAATAAAAT+GGG - chr8_4:25211169-25211188 None:intergenic 30.0%
AGTGAGATTGAGGAATAAAA+TGG - chr8_4:25211170-25211189 None:intergenic 30.0%
! GAGATTTAGGTTTCAATTTG+AGG - chr8_4:25211196-25211215 None:intergenic 30.0%
ATAATCCTTCGATTGAACAA+GGG - chr8_4:25211224-25211243 None:intergenic 30.0%
GCTTAAATGAAACTGGATAA+AGG - chr8_4:25211250-25211269 None:intergenic 30.0%
!! ACGGAATGGGTTAAAATTAT+GGG - chr8_4:25211278-25211297 None:intergenic 30.0%
!! AACGGAATGGGTTAAAATTA+TGG - chr8_4:25211279-25211298 None:intergenic 30.0%
AGCAACTGTAGAAAAGAAAT+GGG - chr8_4:25211347-25211366 None:intergenic 30.0%
TTTCTGCTTAAGATTGAAGA+TGG + chr8_4:25211626-25211645 Msa1320580:three_prime_UTR 30.0%
TTAAGATTGAAGATGGACAA+GGG + chr8_4:25211633-25211652 Msa1320580:three_prime_UTR 30.0%
AAGCATGTGTTTATTGGATT+AGG + chr8_4:25211656-25211675 Msa1320580:three_prime_UTR 30.0%
AGCATGTGTTTATTGGATTA+GGG + chr8_4:25211657-25211676 Msa1320580:three_prime_UTR 30.0%
! GTTTATTGGATTAGGGTTAA+CGG + chr8_4:25211664-25211683 Msa1320580:three_prime_UTR 30.0%
TTAAGCCTATAATTAACAGC+AGG - chr8_4:25211787-25211806 None:intergenic 30.0%
TGCTGTTAATTATAGGCTTA+AGG + chr8_4:25211786-25211805 Msa1320580:three_prime_UTR 30.0%
! TCTCATGTTTGTGAACTTTT+AGG + chr8_4:25212026-25212045 Msa1320580:intron 30.0%
AGTAACACATGTAAAGAGAA+GGG - chr8_4:25212271-25212290 None:intergenic 30.0%
TAGTAACACATGTAAAGAGA+AGG - chr8_4:25212272-25212291 None:intergenic 30.0%
GTTGCAAGCTATAAACTATA+AGG + chr8_4:25212776-25212795 Msa1320580:intron 30.0%
CAGAAAATTGAATGAACAGA+CGG - chr8_4:25212865-25212884 None:intergenic 30.0%
CAATTTCTTCTGTCAATATC+TGG + chr8_4:25213135-25213154 Msa1320580:intron 30.0%
AACTAATGAAGTTACCTCTT+GGG - chr8_4:25213506-25213525 None:intergenic 30.0%
AAACTAATGAAGTTACCTCT+TGG - chr8_4:25213507-25213526 None:intergenic 30.0%
AAGAGGTAACTTCATTAGTT+TGG + chr8_4:25213506-25213525 Msa1320580:intron 30.0%
GCTGAAATTAGAGTTCAAAA+AGG + chr8_4:25213628-25213647 Msa1320580:intron 30.0%
TAAAGTCATTACCCTTAAGT+TGG - chr8_4:25213770-25213789 None:intergenic 30.0%
TCTGCTTTGATAGAGAAAAA+AGG + chr8_4:25213858-25213877 Msa1320580:intron 30.0%
TCAAACCTTGGATATTTCTT+AGG + chr8_4:25214048-25214067 Msa1320580:intron 30.0%
ATTTGCCTAAGAAATATCCA+AGG - chr8_4:25214056-25214075 None:intergenic 30.0%
! TTTGTAGACCTTCAAGTTAT+AGG + chr8_4:25214167-25214186 Msa1320580:three_prime_UTR 30.0%
TAGGATAACCTATAACTTGA+AGG - chr8_4:25214178-25214197 None:intergenic 30.0%
CAAGTTATAGGTTATCCTAT+TGG + chr8_4:25214179-25214198 Msa1320580:three_prime_UTR 30.0%
!!! TTTCATGGTTTTGGAGTTGT+GGG - chr8_4:25211145-25211164 None:intergenic 35.0%
! AAAATGGGGTTTCATGGTTT+TGG - chr8_4:25211154-25211173 None:intergenic 35.0%
AGGAATAAAATGGGGTTTCA+TGG - chr8_4:25211160-25211179 None:intergenic 35.0%
CATAATCCTTCGATTGAACA+AGG - chr8_4:25211225-25211244 None:intergenic 35.0%
AGAAAAGAAATGGGTCGAAA+AGG - chr8_4:25211338-25211357 None:intergenic 35.0%
GAGCAACTGTAGAAAAGAAA+TGG - chr8_4:25211348-25211367 None:intergenic 35.0%
CTTAAGATTGAAGATGGACA+AGG + chr8_4:25211632-25211651 Msa1320580:three_prime_UTR 35.0%
TGTGAGAAGAGAACATGATA+AGG + chr8_4:25211739-25211758 Msa1320580:three_prime_UTR 35.0%
! TAGACATTGGTTAGACACAT+GGG - chr8_4:25212001-25212020 None:intergenic 35.0%
! ATAGACATTGGTTAGACACA+TGG - chr8_4:25212002-25212021 None:intergenic 35.0%
ACATGAGACAGTATAGACAT+TGG - chr8_4:25212014-25212033 None:intergenic 35.0%
TATAGTTGAATACAAGGTCG+TGG + chr8_4:25212290-25212309 Msa1320580:intron 35.0%
AGACATAAGGGCTAAAAGAA+AGG + chr8_4:25212637-25212656 Msa1320580:intron 35.0%
TGATATGCATGTTAAGAACG+AGG - chr8_4:25212687-25212706 None:intergenic 35.0%
CAACACATATATCTTTCCTC+AGG - chr8_4:25212749-25212768 None:intergenic 35.0%
!! AATACCTATGACTTGCGTTT+TGG - chr8_4:25212932-25212951 None:intergenic 35.0%
TCATATGCACATTCAGAAAG+AGG - chr8_4:25212972-25212991 None:intergenic 35.0%
ATTGACACACATTCGTAAAG+AGG - chr8_4:25213119-25213138 None:intergenic 35.0%
TTCTTCTGTCAATATCTGGA+TGG + chr8_4:25213139-25213158 Msa1320580:intron 35.0%
! GTCTTTTCTGATATGTTCTG+CGG + chr8_4:25213161-25213180 Msa1320580:intron 35.0%
!!! TTTTAGAACCTACGCTTCAA+TGG - chr8_4:25213213-25213232 None:intergenic 35.0%
AGCTCTCTTGAAATAGTGTT+AGG + chr8_4:25213478-25213497 Msa1320580:intron 35.0%
! AGGTAACTTCATTAGTTTGG+TGG + chr8_4:25213509-25213528 Msa1320580:intron 35.0%
AAAAGGGCTATGATATCATC+AGG + chr8_4:25213645-25213664 Msa1320580:intron 35.0%
AAAGGGCTATGATATCATCA+GGG + chr8_4:25213646-25213665 Msa1320580:intron 35.0%
AGAAGCTAATGACCAACTTA+AGG + chr8_4:25213755-25213774 Msa1320580:three_prime_UTR 35.0%
GAAGCTAATGACCAACTTAA+GGG + chr8_4:25213756-25213775 Msa1320580:three_prime_UTR 35.0%
CTTAAACATGTGTACACCAA+AGG - chr8_4:25213995-25214014 None:intergenic 35.0%
!!! GTTTCATGGTTTTGGAGTTG+TGG - chr8_4:25211146-25211165 None:intergenic 40.0%
TTTGAGGGAAAGTGAGATTG+AGG - chr8_4:25211180-25211199 None:intergenic 40.0%
AACAAGGGTGGTAGAGATTT+AGG - chr8_4:25211209-25211228 None:intergenic 40.0%
GGTGAGAGCTTAAATGAAAC+TGG - chr8_4:25211257-25211276 None:intergenic 40.0%
AGTGAAGAACTGTGGAGAAA+CGG - chr8_4:25211297-25211316 None:intergenic 40.0%
CAACAAAGAGTGAAGAACTG+TGG - chr8_4:25211305-25211324 None:intergenic 40.0%
! CAAGGGAAGCATGTGTTTAT+TGG + chr8_4:25211650-25211669 Msa1320580:three_prime_UTR 40.0%
AGAGAACATGATAAGGAGTC+TGG + chr8_4:25211746-25211765 Msa1320580:three_prime_UTR 40.0%
AATTAACAGCAGGATGGCAT+TGG - chr8_4:25211777-25211796 None:intergenic 40.0%
GCCATCCTGCTGTTAATTAT+AGG + chr8_4:25211779-25211798 Msa1320580:three_prime_UTR 40.0%
GCCTATAATTAACAGCAGGA+TGG - chr8_4:25211783-25211802 None:intergenic 40.0%
!! TTTGTTTCCAGGGTACTGTT+AGG + chr8_4:25211943-25211962 Msa1320580:intron 40.0%
GTAAGACAGTTGAAGAAGTG+TGG - chr8_4:25212230-25212249 None:intergenic 40.0%
CTGTCTTACATCACATCTCA+AGG + chr8_4:25212241-25212260 Msa1320580:three_prime_UTR 40.0%
TTTCAACCAAATGAGCTCAC+AGG - chr8_4:25212325-25212344 None:intergenic 40.0%
CTCATGAAAAAACAGACCAG+AGG - chr8_4:25212427-25212446 None:intergenic 40.0%
ATTCCTTCTAATGCAACCTG+AGG + chr8_4:25212730-25212749 Msa1320580:intron 40.0%
! TTTCCTCAGGTTGCATTAGA+AGG - chr8_4:25212736-25212755 None:intergenic 40.0%
CTCTGATTATCTTTAGCGAC+AGG + chr8_4:25213008-25213027 Msa1320580:intron 40.0%
TTGCAAAACCATTGAAGCGT+AGG + chr8_4:25213202-25213221 Msa1320580:intron 40.0%
TGCTATGCTCATTCAGATAG+AGG - chr8_4:25213268-25213287 None:intergenic 40.0%
AATAGTGTTAGGAACCCAAG+AGG + chr8_4:25213489-25213508 Msa1320580:intron 40.0%
TGATATCATCAGGGTGAGTT+TGG + chr8_4:25213655-25213674 Msa1320580:intron 40.0%
! CTAGTTGTTCTTTTGTCGAC+AGG + chr8_4:25213689-25213708 Msa1320580:intron 40.0%
ACAGGAGTTGAAGTAGAGAA+AGG + chr8_4:25213707-25213726 Msa1320580:intron 40.0%
GATGATGCTTTCACCGTTAA+TGG - chr8_4:25213840-25213859 None:intergenic 40.0%
! AAAGGCATGTGTTACGTCAA+TGG + chr8_4:25213876-25213895 Msa1320580:three_prime_UTR 40.0%
TGATAGCTTGACATTGCCTT+TGG + chr8_4:25213976-25213995 Msa1320580:intron 40.0%
TTAGGCAAATCAACAACTGC+TGG + chr8_4:25214066-25214085 Msa1320580:intron 40.0%
TGTGGGAACGAGACTGAAAT+TGG - chr8_4:25211128-25211147 None:intergenic 45.0%
TACCACCCTTGTTCAATCGA+AGG + chr8_4:25211216-25211235 Msa1320580:five_prime_UTR 45.0%
ATCCTTCGATTGAACAAGGG+TGG - chr8_4:25211221-25211240 None:intergenic 45.0%
GAACTGTGGAGAAACGGAAT+GGG - chr8_4:25211291-25211310 None:intergenic 45.0%
AGAACTGTGGAGAAACGGAA+TGG - chr8_4:25211292-25211311 None:intergenic 45.0%
GGTTCCAATCAGACAAGATC+TGG + chr8_4:25211456-25211475 Msa1320580:three_prime_UTR 45.0%
CAATCAGACAAGATCTGGTC+CGG + chr8_4:25211461-25211480 Msa1320580:three_prime_UTR 45.0%
CTTCTAACCGTATCTCACAC+CGG - chr8_4:25211514-25211533 None:intergenic 45.0%
CGGTTAGAAGATCCCAACTT+CGG + chr8_4:25211524-25211543 Msa1320580:three_prime_UTR 45.0%
! TGTTTTTCCCTGTCAGTGTG+AGG + chr8_4:25211568-25211587 Msa1320580:three_prime_UTR 45.0%
! GTTTTTCCCTGTCAGTGTGA+GGG + chr8_4:25211569-25211588 Msa1320580:three_prime_UTR 45.0%
! TTGGATTAGGGTTAACGGAG+AGG + chr8_4:25211669-25211688 Msa1320580:three_prime_UTR 45.0%
AAGTTTGCCTAACAGTACCC+TGG - chr8_4:25211953-25211972 None:intergenic 45.0%
TGAATACAAGGTCGTGGCAT+TGG + chr8_4:25212296-25212315 Msa1320580:intron 45.0%
AGATTTAGCCTGTCGAGTGA+TGG + chr8_4:25212896-25212915 Msa1320580:intron 45.0%
GTAATGTTCCATCACTCGAC+AGG - chr8_4:25212907-25212926 None:intergenic 45.0%
ACTGCCAAAACGCAAGTCAT+AGG + chr8_4:25212925-25212944 Msa1320580:three_prime_UTR 45.0%
! CATACTCTGTGTCGGTACAA+AGG - chr8_4:25213035-25213054 None:intergenic 45.0%
!!! ACTTTAAAATAATATTTTAT+TGG + chr8_4:25212475-25212494 Msa1320580:intron 5.0%
!!! TTTTTAACAATATTATATTT+TGG - chr8_4:25213387-25213406 None:intergenic 5.0%
TTAGCCGCATTTGTAACCGC+CGG - chr8_4:25211435-25211454 None:intergenic 50.0%
GCGGTTACAAATGCGGCTAA+TGG + chr8_4:25211435-25211454 Msa1320580:three_prime_UTR 50.0%
CGGACCAGATCTTGTCTGAT+TGG - chr8_4:25211463-25211482 None:intergenic 50.0%
ACTGAACCCTCACACTGACA+GGG - chr8_4:25211578-25211597 None:intergenic 50.0%
AACTGAACCCTCACACTGAC+AGG - chr8_4:25211579-25211598 None:intergenic 50.0%
GTGTGAGGGTTCAGTTGAAC+TGG + chr8_4:25211583-25211602 Msa1320580:three_prime_UTR 50.0%
TGGACAACGACAACACTCGA+CGG - chr8_4:25212210-25212229 None:intergenic 50.0%
TGGTGACCTGTGAGCTCATT+TGG + chr8_4:25212316-25212335 Msa1320580:intron 50.0%
GCGGCATTGCATATTACCTC+TGG + chr8_4:25212408-25212427 Msa1320580:intron 50.0%
GACGGACATGCATGTGCAAA+AGG - chr8_4:25212847-25212866 None:intergenic 50.0%
TCAACAACTGCTGGTGTACG+TGG + chr8_4:25214075-25214094 Msa1320580:three_prime_UTR 50.0%
TTCAGCACTGCTAAGACTCG+AGG + chr8_4:25214107-25214126 Msa1320580:three_prime_UTR 50.0%
ACCTCCGGCGGTTACAAATG+CGG + chr8_4:25211428-25211447 Msa1320580:three_prime_UTR 55.0%
ACCGTATCTCACACCGGTCA+CGG - chr8_4:25211508-25211527 None:intergenic 55.0%
GCGAAGAGATCGCCGAAGTT+GGG - chr8_4:25211539-25211558 None:intergenic 55.0%
AGCGAAGAGATCGCCGAAGT+TGG - chr8_4:25211540-25211559 None:intergenic 55.0%
ATCCCGCCACGAACAACCTC+CGG + chr8_4:25211413-25211432 Msa1320580:three_prime_UTR 60.0%
TAACCGCCGGAGGTTGTTCG+TGG - chr8_4:25211422-25211441 None:intergenic 60.0%
TATCTCACACCGGTCACGGC+GGG - chr8_4:25211504-25211523 None:intergenic 60.0%
GTATCTCACACCGGTCACGG+CGG - chr8_4:25211505-25211524 None:intergenic 60.0%
GCCGTGACCGGTGTGAGATA+CGG + chr8_4:25211504-25211523 Msa1320580:three_prime_UTR 60.0%
GCCGCATTTGTAACCGCCGG+AGG - chr8_4:25211432-25211451 None:intergenic 65.0%
CGCCGGAGGTTGTTCGTGGC+GGG - chr8_4:25211418-25211437 None:intergenic 70.0%
CCGCCGGAGGTTGTTCGTGG+CGG - chr8_4:25211419-25211438 None:intergenic 70.0%
CCGCCACGAACAACCTCCGG+CGG + chr8_4:25211416-25211435 Msa1320580:three_prime_UTR 70.0%
GGACACAACGCGGAGACGGC+CGG - chr8_4:25211483-25211502 None:intergenic 70.0%
GGCGGGACACAACGCGGAGA+CGG - chr8_4:25211487-25211506 None:intergenic 70.0%
GGTCACGGCGGGACACAACG+CGG - chr8_4:25211493-25211512 None:intergenic 70.0%
GCGTTGTGTCCCGCCGTGAC+CGG + chr8_4:25211492-25211511 Msa1320580:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 25211087 25214351 25211087 ID=Msa1320580;Name=Msa1320580
chr8_4 mRNA 25211087 25214351 25211087 ID=Msa1320580-mRNA-1;Parent=Msa1320580;Name=Msa1320580-mRNA-1;_AED=0.26;_eAED=0.26;_QI=155|1|1|1|0.2|0.33|6|961|51
chr8_4 exon 25211087 25211807 25211087 ID=Msa1320580-mRNA-1:exon:8622;Parent=Msa1320580-mRNA-1
chr8_4 exon 25212188 25212262 25212188 ID=Msa1320580-mRNA-1:exon:8623;Parent=Msa1320580-mRNA-1
chr8_4 exon 25212898 25212946 25212898 ID=Msa1320580-mRNA-1:exon:8624;Parent=Msa1320580-mRNA-1
chr8_4 exon 25213711 25213777 25213711 ID=Msa1320580-mRNA-1:exon:8625;Parent=Msa1320580-mRNA-1
chr8_4 exon 25213871 25213948 25213871 ID=Msa1320580-mRNA-1:exon:8626;Parent=Msa1320580-mRNA-1
chr8_4 exon 25214070 25214351 25214070 ID=Msa1320580-mRNA-1:exon:8627;Parent=Msa1320580-mRNA-1
chr8_4 five_prime_UTR 25211087 25211241 25211087 ID=Msa1320580-mRNA-1:five_prime_utr;Parent=Msa1320580-mRNA-1
chr8_4 CDS 25211242 25211397 25211242 ID=Msa1320580-mRNA-1:cds;Parent=Msa1320580-mRNA-1
chr8_4 three_prime_UTR 25211398 25211807 25211398 ID=Msa1320580-mRNA-1:three_prime_utr;Parent=Msa1320580-mRNA-1
chr8_4 three_prime_UTR 25212188 25212262 25212188 ID=Msa1320580-mRNA-1:three_prime_utr;Parent=Msa1320580-mRNA-1
chr8_4 three_prime_UTR 25212898 25212946 25212898 ID=Msa1320580-mRNA-1:three_prime_utr;Parent=Msa1320580-mRNA-1
chr8_4 three_prime_UTR 25213711 25213777 25213711 ID=Msa1320580-mRNA-1:three_prime_utr;Parent=Msa1320580-mRNA-1
chr8_4 three_prime_UTR 25213871 25213948 25213871 ID=Msa1320580-mRNA-1:three_prime_utr;Parent=Msa1320580-mRNA-1
chr8_4 three_prime_UTR 25214070 25214351 25214070 ID=Msa1320580-mRNA-1:three_prime_utr;Parent=Msa1320580-mRNA-1
Gene Sequence

>Msa1320580

ATGTTCCTTTATCCAGTTTCATTTAAGCTCTCACCCATAATTTTAACCCATTCCGTTTCTCCACAGTTCTTCACTCTTTGTTGTTCTTCAAATCCTTTTCGACCCATTTCTTTTCTACAGTTGCTCAGTTCCTTTTCTTCCAAAACATATATATAG
Protein sequence

>Msa1320580

MFLYPVSFKLSPIILTHSVSPQFFTLCCSSNPFRPISFLQLLSSFSSKTYI*