AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0990830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 95 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAAAACTTAAGAATTAAT+TGG 0.153202 6_4:+53523905 Msa0990830:three_prime_UTR
AACGGAATGGGTTAAAATTA+TGG 0.195683 6_4:-53521046 None:intergenic
ACTCTTCACATTAATGCTTT+TGG 0.207367 6_4:+53520848 None:intergenic
AAAATGGGGTTTCATGGTTT+TGG 0.235392 6_4:-53520921 None:intergenic
AACAAGGGTGGTAGAGATTT+AGG 0.237128 6_4:-53520976 None:intergenic
CAAGGGAAGCATGTGTTTAT+TGG 0.237220 6_4:+53521420 Msa0990830:three_prime_UTR
GCCATCCTGCTGTTAATTAT+AGG 0.242602 6_4:+53521549 Msa0990830:three_prime_UTR
TTTGTAGACCTTCAAGTTAT+AGG 0.264756 6_4:+53523937 Msa0990830:three_prime_UTR
AAGCATGTGTTTATTGGATT+AGG 0.266751 6_4:+53521426 Msa0990830:three_prime_UTR
TATTTCTTAGTTGGTACATT+TGG 0.267786 6_4:+53521948 Msa0990830:intron
AGTGAGATTGAGGAATAAAA+TGG 0.312064 6_4:-53520937 None:intergenic
AGGAATAAAATGGGGTTTCA+TGG 0.315510 6_4:-53520927 None:intergenic
CAAGTTATAGGTTATCCTAT+TGG 0.318712 6_4:+53523949 Msa0990830:three_prime_UTR
ATTTCTTAGTTGGTACATTT+GGG 0.325548 6_4:+53521949 Msa0990830:intron
AGCATGTGTTTATTGGATTA+GGG 0.332772 6_4:+53521427 Msa0990830:three_prime_UTR
CTTGTCCTATATATATGTTT+TGG 0.337556 6_4:-53521151 None:intergenic
TGCTGTTAATTATAGGCTTA+AGG 0.347777 6_4:+53521556 Msa0990830:three_prime_UTR
TTCTTCCAAAACATATATAT+AGG 0.349435 6_4:+53521146 Msa0990830:CDS
CTTCTAAAGATTGTGTATAA+TGG 0.357548 6_4:+53523697 Msa0990830:three_prime_UTR
GAGCAACTGTAGAAAAGAAA+TGG 0.357579 6_4:-53521115 None:intergenic
TTAAAACTTAAGAATTAATT+GGG 0.366320 6_4:+53523906 Msa0990830:three_prime_UTR
TGTGGGAACGAGACTGAAAT+TGG 0.369214 6_4:-53520895 None:intergenic
GGTGAGAGCTTAAATGAAAC+TGG 0.376751 6_4:-53521024 None:intergenic
AATACCTATGACTTGCGTTT+TGG 0.377633 6_4:-53522699 None:intergenic
GAGATTTAGGTTTCAATTTG+AGG 0.394504 6_4:-53520963 None:intergenic
AGAAGCTAATGACCAACTTA+AGG 0.395849 6_4:+53523525 Msa0990830:three_prime_UTR
ACGGAATGGGTTAAAATTAT+GGG 0.402922 6_4:-53521045 None:intergenic
AGTATTATCAATCATCCAAT+AGG 0.414360 6_4:-53523964 None:intergenic
AACTGAACCCTCACACTGAC+AGG 0.423043 6_4:-53521346 None:intergenic
GTGAGATTGAGGAATAAAAT+GGG 0.426659 6_4:-53520936 None:intergenic
TGTGAGAAGAGAACATGATA+AGG 0.442504 6_4:+53521509 Msa0990830:three_prime_UTR
GAAGCTAATGACCAACTTAA+GGG 0.443177 6_4:+53523526 Msa0990830:three_prime_UTR
GCTTAAATGAAACTGGATAA+AGG 0.443636 6_4:-53521017 None:intergenic
GCGTTGTGTCCCGCCGTGAC+CGG 0.447886 6_4:+53521262 Msa0990830:three_prime_UTR
GTGTGAGGGTTCAGTTGAAC+TGG 0.448765 6_4:+53521353 Msa0990830:three_prime_UTR
TAGGATAACCTATAACTTGA+AGG 0.448867 6_4:-53523945 None:intergenic
ACTGCCAAAACGCAAGTCAT+AGG 0.449501 6_4:+53522695 Msa0990830:three_prime_UTR
AGATTTAGGTTTCAATTTGA+GGG 0.451063 6_4:-53520962 None:intergenic
TTTCTGCTTAAGATTGAAGA+TGG 0.451141 6_4:+53521396 Msa0990830:three_prime_UTR
GTTTATTGGATTAGGGTTAA+CGG 0.452324 6_4:+53521434 Msa0990830:three_prime_UTR
CGCCGGAGGTTGTTCGTGGC+GGG 0.452665 6_4:-53521185 None:intergenic
AAAGGCATGTGTTACGTCAA+TGG 0.457053 6_4:+53523646 Msa0990830:three_prime_UTR
AGCGAAGAGATCGCCGAAGT+TGG 0.459540 6_4:-53521307 None:intergenic
GGTTCCAATCAGACAAGATC+TGG 0.463783 6_4:+53521226 Msa0990830:three_prime_UTR
AGAGAACATGATAAGGAGTC+TGG 0.464167 6_4:+53521516 Msa0990830:three_prime_UTR
GCGAAGAGATCGCCGAAGTT+GGG 0.467204 6_4:-53521306 None:intergenic
GCCGTGACCGGTGTGAGATA+CGG 0.467324 6_4:+53521274 Msa0990830:three_prime_UTR
GGACACAACGCGGAGACGGC+CGG 0.477578 6_4:-53521250 None:intergenic
TTTGAGGGAAAGTGAGATTG+AGG 0.478972 6_4:-53520947 None:intergenic
ATCCCGCCACGAACAACCTC+CGG 0.483393 6_4:+53521183 Msa0990830:three_prime_UTR
TTAGGCAAATCAACAACTGC+TGG 0.486080 6_4:+53523836 Msa0990830:intron
TAACCGCCGGAGGTTGTTCG+TGG 0.487228 6_4:-53521189 None:intergenic
CGGACCAGATCTTGTCTGAT+TGG 0.492781 6_4:-53521230 None:intergenic
TAAAGTCATTACCCTTAAGT+TGG 0.492910 6_4:-53523537 None:intergenic
AGTGAAGAACTGTGGAGAAA+CGG 0.493197 6_4:-53521064 None:intergenic
TTTCAATTTGAATTGTACTC+TGG 0.494796 6_4:+53524032 Msa0990830:three_prime_UTR
GCGGTTACAAATGCGGCTAA+TGG 0.502661 6_4:+53521205 Msa0990830:three_prime_UTR
AGAAAAGAAATGGGTCGAAA+AGG 0.503425 6_4:-53521105 None:intergenic
CAATCAGACAAGATCTGGTC+CGG 0.506501 6_4:+53521231 Msa0990830:three_prime_UTR
GGCGGGACACAACGCGGAGA+CGG 0.509066 6_4:-53521254 None:intergenic
AGCAACTGTAGAAAAGAAAT+GGG 0.514548 6_4:-53521114 None:intergenic
AATTAACAGCAGGATGGCAT+TGG 0.517260 6_4:-53521544 None:intergenic
AGATTTAGCCTGTCGAGTGA+TGG 0.527799 6_4:+53522666 Msa0990830:intron
ACCGTATCTCACACCGGTCA+CGG 0.531370 6_4:-53521275 None:intergenic
CATAATCCTTCGATTGAACA+AGG 0.536572 6_4:-53520992 None:intergenic
TATCTCACACCGGTCACGGC+GGG 0.540870 6_4:-53521271 None:intergenic
GTAATGTTCCATCACTCGAC+AGG 0.545474 6_4:-53522674 None:intergenic
ACAGGAGTTGAAGTAGAGAA+AGG 0.547321 6_4:+53523477 Msa0990830:intron
TACCACCCTTGTTCAATCGA+AGG 0.561434 6_4:+53520986 Msa0990830:five_prime_UTR
CCGCCGGAGGTTGTTCGTGG+CGG 0.571890 6_4:-53521186 None:intergenic
CTGTCTTACATCACATCTCA+AGG 0.573407 6_4:+53522011 Msa0990830:three_prime_UTR
TTAAGCCTATAATTAACAGC+AGG 0.578878 6_4:-53521554 None:intergenic
CTTAAGATTGAAGATGGACA+AGG 0.579822 6_4:+53521402 Msa0990830:three_prime_UTR
TCAACAACTGCTGGTGTACG+TGG 0.580588 6_4:+53523845 Msa0990830:three_prime_UTR
GAACTGTGGAGAAACGGAAT+GGG 0.584247 6_4:-53521058 None:intergenic
AGAACTGTGGAGAAACGGAA+TGG 0.592306 6_4:-53521059 None:intergenic
TTGGATTAGGGTTAACGGAG+AGG 0.594029 6_4:+53521439 Msa0990830:three_prime_UTR
TGAGATTGAGGAATAAAATG+GGG 0.611914 6_4:-53520935 None:intergenic
GCCGCATTTGTAACCGCCGG+AGG 0.612310 6_4:-53521199 None:intergenic
ACCTCCGGCGGTTACAAATG+CGG 0.616884 6_4:+53521198 Msa0990830:three_prime_UTR
ACTGAACCCTCACACTGACA+GGG 0.621101 6_4:-53521345 None:intergenic
TTAGCCGCATTTGTAACCGC+CGG 0.625611 6_4:-53521202 None:intergenic
GCCTATAATTAACAGCAGGA+TGG 0.636206 6_4:-53521550 None:intergenic
ATCCTTCGATTGAACAAGGG+TGG 0.639676 6_4:-53520988 None:intergenic
CGGTTAGAAGATCCCAACTT+CGG 0.643782 6_4:+53521294 Msa0990830:three_prime_UTR
CAACAAAGAGTGAAGAACTG+TGG 0.649585 6_4:-53521072 None:intergenic
TTCAGCACTGCTAAGACTCG+AGG 0.650870 6_4:+53523877 Msa0990830:three_prime_UTR
GTAAGACAGTTGAAGAAGTG+TGG 0.654301 6_4:-53521997 None:intergenic
TTAAGATTGAAGATGGACAA+GGG 0.676442 6_4:+53521403 Msa0990830:three_prime_UTR
TGGACAACGACAACACTCGA+CGG 0.686164 6_4:-53521977 None:intergenic
CTTCTAACCGTATCTCACAC+CGG 0.687609 6_4:-53521281 None:intergenic
CCGCCACGAACAACCTCCGG+CGG 0.697916 6_4:+53521186 Msa0990830:three_prime_UTR
GGTCACGGCGGGACACAACG+CGG 0.706808 6_4:-53521260 None:intergenic
ATAATCCTTCGATTGAACAA+GGG 0.716702 6_4:-53520991 None:intergenic
GTATCTCACACCGGTCACGG+CGG 0.748302 6_4:-53521272 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAACTTAAGAATTAAT+TGG + chr6_4:53523905-53523924 Msa0990830:three_prime_UTR 10.0%
!! TTAAAACTTAAGAATTAATT+GGG + chr6_4:53523906-53523925 Msa0990830:three_prime_UTR 10.0%
!!! ACTATTTTTCAACTAAAAAT+TGG - chr6_4:53521645-53521664 None:intergenic 15.0%
!! AACTTAAACTTTGTAAAAAT+AGG + chr6_4:53521738-53521757 Msa0990830:intron 15.0%
!! TACAATTAGAAAATTGAATA+AGG - chr6_4:53522336-53522355 None:intergenic 15.0%
!! TTATAAAAAAATGAGACATA+AGG + chr6_4:53522394-53522413 Msa0990830:intron 15.0%
!! TATAAAAAAATGAGACATAA+GGG + chr6_4:53522395-53522414 Msa0990830:intron 15.0%
!! AGTAAAACTAAAAAATAAGA+TGG - chr6_4:53523333-53523352 None:intergenic 15.0%
!!! TATATATGTTTTGGAAGAAA+AGG - chr6_4:53521145-53521164 None:intergenic 20.0%
!! TTCTTCCAAAACATATATAT+AGG + chr6_4:53521146-53521165 Msa0990830:CDS 20.0%
!!! TAATATGATTTGTGAAATGA+AGG + chr6_4:53522361-53522380 Msa0990830:intron 20.0%
!!! AATATGATTTGTGAAATGAA+GGG + chr6_4:53522362-53522381 Msa0990830:intron 20.0%
!!! CTTTATTTCGAATTTGTTAA+TGG + chr6_4:53523351-53523370 Msa0990830:intron 20.0%
!!! AACAATTTTTATGCCATTAA+CGG + chr6_4:53523594-53523613 Msa0990830:intron 20.0%
!!! ATTTTGTACAAAAAAACACA+AGG - chr6_4:53523799-53523818 None:intergenic 20.0%
!!! GTTAGATTTTATGTTTTTGA+TGG + chr6_4:53523997-53524016 Msa0990830:three_prime_UTR 20.0%
!! AGATTTAGGTTTCAATTTGA+GGG - chr6_4:53520965-53520984 None:intergenic 25.0%
!! CTTGTCCTATATATATGTTT+TGG - chr6_4:53521154-53521173 None:intergenic 25.0%
!! TATGCATAAAATTTGTTTCC+AGG + chr6_4:53521702-53521721 Msa0990830:intron 25.0%
!! ATGCATAAAATTTGTTTCCA+GGG + chr6_4:53521703-53521722 Msa0990830:intron 25.0%
! TTGTTGTGATATTTCTTAGT+TGG + chr6_4:53521939-53521958 Msa0990830:intron 25.0%
!! TATTTCTTAGTTGGTACATT+TGG + chr6_4:53521948-53521967 Msa0990830:intron 25.0%
!! ATTTCTTAGTTGGTACATTT+GGG + chr6_4:53521949-53521968 Msa0990830:intron 25.0%
! TGTTACTATAGTTGAATACA+AGG + chr6_4:53522054-53522073 Msa0990830:intron 25.0%
! TCTTAAAAAGACAACACAAA+TGG + chr6_4:53522134-53522153 Msa0990830:intron 25.0%
! CTAATATGTGAATAATCAAG+CGG + chr6_4:53522159-53522178 Msa0990830:intron 25.0%
! TTATAATACATACTCTGTGT+CGG - chr6_4:53522813-53522832 None:intergenic 25.0%
!! ACATTTTACTGAAGAAATTC+AGG - chr6_4:53523013-53523032 None:intergenic 25.0%
!!! TATATATGCTTTGTTTTGAC+AGG - chr6_4:53523101-53523120 None:intergenic 25.0%
! CAAAGCATATATATACGTAT+TGG + chr6_4:53523108-53523127 Msa0990830:intron 25.0%
! CTGAAATTAGAGTTCAAAAA+GGG + chr6_4:53523399-53523418 Msa0990830:intron 25.0%
!! CTTCTAAAGATTGTGTATAA+TGG + chr6_4:53523697-53523716 Msa0990830:three_prime_UTR 25.0%
!! TTTTGTACAAAATCAAACCT+TGG + chr6_4:53523806-53523825 Msa0990830:intron 25.0%
! AGTATTATCAATCATCCAAT+AGG - chr6_4:53523967-53523986 None:intergenic 25.0%
!!! TATGTTTTTGATGGAACTAT+TGG + chr6_4:53524006-53524025 Msa0990830:three_prime_UTR 25.0%
! TTTCAATTTGAATTGTACTC+TGG + chr6_4:53524032-53524051 Msa0990830:three_prime_UTR 25.0%
TGAGATTGAGGAATAAAATG+GGG - chr6_4:53520938-53520957 None:intergenic 30.0%
GTGAGATTGAGGAATAAAAT+GGG - chr6_4:53520939-53520958 None:intergenic 30.0%
AGTGAGATTGAGGAATAAAA+TGG - chr6_4:53520940-53520959 None:intergenic 30.0%
! GAGATTTAGGTTTCAATTTG+AGG - chr6_4:53520966-53520985 None:intergenic 30.0%
ATAATCCTTCGATTGAACAA+GGG - chr6_4:53520994-53521013 None:intergenic 30.0%
GCTTAAATGAAACTGGATAA+AGG - chr6_4:53521020-53521039 None:intergenic 30.0%
!! ACGGAATGGGTTAAAATTAT+GGG - chr6_4:53521048-53521067 None:intergenic 30.0%
!! AACGGAATGGGTTAAAATTA+TGG - chr6_4:53521049-53521068 None:intergenic 30.0%
AGCAACTGTAGAAAAGAAAT+GGG - chr6_4:53521117-53521136 None:intergenic 30.0%
TTTCTGCTTAAGATTGAAGA+TGG + chr6_4:53521396-53521415 Msa0990830:three_prime_UTR 30.0%
TTAAGATTGAAGATGGACAA+GGG + chr6_4:53521403-53521422 Msa0990830:three_prime_UTR 30.0%
AAGCATGTGTTTATTGGATT+AGG + chr6_4:53521426-53521445 Msa0990830:three_prime_UTR 30.0%
AGCATGTGTTTATTGGATTA+GGG + chr6_4:53521427-53521446 Msa0990830:three_prime_UTR 30.0%
! GTTTATTGGATTAGGGTTAA+CGG + chr6_4:53521434-53521453 Msa0990830:three_prime_UTR 30.0%
TTAAGCCTATAATTAACAGC+AGG - chr6_4:53521557-53521576 None:intergenic 30.0%
TGCTGTTAATTATAGGCTTA+AGG + chr6_4:53521556-53521575 Msa0990830:three_prime_UTR 30.0%
! TCTCATGTTTGTGAACTTTT+AGG + chr6_4:53521796-53521815 Msa0990830:intron 30.0%
AGTAACACATGTAAAGAGAA+GGG - chr6_4:53522041-53522060 None:intergenic 30.0%
TAGTAACACATGTAAAGAGA+AGG - chr6_4:53522042-53522061 None:intergenic 30.0%
GTTGCAAGCTATAAACTATA+AGG + chr6_4:53522546-53522565 Msa0990830:intron 30.0%
CAGAAAATTGAATGAACAGA+CGG - chr6_4:53522635-53522654 None:intergenic 30.0%
CAATTTCTTCTGTCAATATC+TGG + chr6_4:53522905-53522924 Msa0990830:intron 30.0%
AACTAATGAAGTTACCTCTT+GGG - chr6_4:53523276-53523295 None:intergenic 30.0%
AAACTAATGAAGTTACCTCT+TGG - chr6_4:53523277-53523296 None:intergenic 30.0%
AAGAGGTAACTTCATTAGTT+TGG + chr6_4:53523276-53523295 Msa0990830:intron 30.0%
GCTGAAATTAGAGTTCAAAA+AGG + chr6_4:53523398-53523417 Msa0990830:intron 30.0%
TAAAGTCATTACCCTTAAGT+TGG - chr6_4:53523540-53523559 None:intergenic 30.0%
TCTGCTTTGATAGAGAAAAA+AGG + chr6_4:53523628-53523647 Msa0990830:intron 30.0%
TCAAACCTTGGATATTTCTT+AGG + chr6_4:53523818-53523837 Msa0990830:intron 30.0%
ATTTGCCTAAGAAATATCCA+AGG - chr6_4:53523826-53523845 None:intergenic 30.0%
! TTTGTAGACCTTCAAGTTAT+AGG + chr6_4:53523937-53523956 Msa0990830:three_prime_UTR 30.0%
TAGGATAACCTATAACTTGA+AGG - chr6_4:53523948-53523967 None:intergenic 30.0%
CAAGTTATAGGTTATCCTAT+TGG + chr6_4:53523949-53523968 Msa0990830:three_prime_UTR 30.0%
!!! TTTCATGGTTTTGGAGTTGT+GGG - chr6_4:53520915-53520934 None:intergenic 35.0%
! AAAATGGGGTTTCATGGTTT+TGG - chr6_4:53520924-53520943 None:intergenic 35.0%
AGGAATAAAATGGGGTTTCA+TGG - chr6_4:53520930-53520949 None:intergenic 35.0%
CATAATCCTTCGATTGAACA+AGG - chr6_4:53520995-53521014 None:intergenic 35.0%
AGAAAAGAAATGGGTCGAAA+AGG - chr6_4:53521108-53521127 None:intergenic 35.0%
GAGCAACTGTAGAAAAGAAA+TGG - chr6_4:53521118-53521137 None:intergenic 35.0%
CTTAAGATTGAAGATGGACA+AGG + chr6_4:53521402-53521421 Msa0990830:three_prime_UTR 35.0%
TGTGAGAAGAGAACATGATA+AGG + chr6_4:53521509-53521528 Msa0990830:three_prime_UTR 35.0%
! TAGACATTGGTTAGACACAT+GGG - chr6_4:53521771-53521790 None:intergenic 35.0%
! ATAGACATTGGTTAGACACA+TGG - chr6_4:53521772-53521791 None:intergenic 35.0%
ACATGAGACAGTATAGACAT+TGG - chr6_4:53521784-53521803 None:intergenic 35.0%
TATAGTTGAATACAAGGTCG+TGG + chr6_4:53522060-53522079 Msa0990830:intron 35.0%
AGACATAAGGGCTAAAAGAA+AGG + chr6_4:53522407-53522426 Msa0990830:intron 35.0%
TGATATGCATGTTAAGAACG+AGG - chr6_4:53522457-53522476 None:intergenic 35.0%
CAACACATATATCTTTCCTC+AGG - chr6_4:53522519-53522538 None:intergenic 35.0%
!! AATACCTATGACTTGCGTTT+TGG - chr6_4:53522702-53522721 None:intergenic 35.0%
TCATATGCACATTCAGAAAG+AGG - chr6_4:53522742-53522761 None:intergenic 35.0%
ATTGACACACATTCGTAAAG+AGG - chr6_4:53522889-53522908 None:intergenic 35.0%
TTCTTCTGTCAATATCTGGA+TGG + chr6_4:53522909-53522928 Msa0990830:intron 35.0%
! GTCTTTTCTGATATGTTCTG+CGG + chr6_4:53522931-53522950 Msa0990830:intron 35.0%
!!! TTTTAGAACCTACGCTTCAA+TGG - chr6_4:53522983-53523002 None:intergenic 35.0%
AGCTCTCTTGAAATAGTGTT+AGG + chr6_4:53523248-53523267 Msa0990830:intron 35.0%
! AGGTAACTTCATTAGTTTGG+TGG + chr6_4:53523279-53523298 Msa0990830:intron 35.0%
AAAAGGGCTATGATATCATC+AGG + chr6_4:53523415-53523434 Msa0990830:intron 35.0%
AAAGGGCTATGATATCATCA+GGG + chr6_4:53523416-53523435 Msa0990830:intron 35.0%
AGAAGCTAATGACCAACTTA+AGG + chr6_4:53523525-53523544 Msa0990830:three_prime_UTR 35.0%
GAAGCTAATGACCAACTTAA+GGG + chr6_4:53523526-53523545 Msa0990830:three_prime_UTR 35.0%
CTTAAACATGTGTACACCAA+AGG - chr6_4:53523765-53523784 None:intergenic 35.0%
!!! GTTTCATGGTTTTGGAGTTG+TGG - chr6_4:53520916-53520935 None:intergenic 40.0%
TTTGAGGGAAAGTGAGATTG+AGG - chr6_4:53520950-53520969 None:intergenic 40.0%
AACAAGGGTGGTAGAGATTT+AGG - chr6_4:53520979-53520998 None:intergenic 40.0%
GGTGAGAGCTTAAATGAAAC+TGG - chr6_4:53521027-53521046 None:intergenic 40.0%
AGTGAAGAACTGTGGAGAAA+CGG - chr6_4:53521067-53521086 None:intergenic 40.0%
CAACAAAGAGTGAAGAACTG+TGG - chr6_4:53521075-53521094 None:intergenic 40.0%
! CAAGGGAAGCATGTGTTTAT+TGG + chr6_4:53521420-53521439 Msa0990830:three_prime_UTR 40.0%
AGAGAACATGATAAGGAGTC+TGG + chr6_4:53521516-53521535 Msa0990830:three_prime_UTR 40.0%
AATTAACAGCAGGATGGCAT+TGG - chr6_4:53521547-53521566 None:intergenic 40.0%
GCCATCCTGCTGTTAATTAT+AGG + chr6_4:53521549-53521568 Msa0990830:three_prime_UTR 40.0%
GCCTATAATTAACAGCAGGA+TGG - chr6_4:53521553-53521572 None:intergenic 40.0%
!! TTTGTTTCCAGGGTACTGTT+AGG + chr6_4:53521713-53521732 Msa0990830:intron 40.0%
GTAAGACAGTTGAAGAAGTG+TGG - chr6_4:53522000-53522019 None:intergenic 40.0%
CTGTCTTACATCACATCTCA+AGG + chr6_4:53522011-53522030 Msa0990830:three_prime_UTR 40.0%
TTTCAACCAAATGAGCTCAC+AGG - chr6_4:53522095-53522114 None:intergenic 40.0%
CTCATGAAAAAACAGACCAG+AGG - chr6_4:53522197-53522216 None:intergenic 40.0%
ATTCCTTCTAATGCAACCTG+AGG + chr6_4:53522500-53522519 Msa0990830:intron 40.0%
! TTTCCTCAGGTTGCATTAGA+AGG - chr6_4:53522506-53522525 None:intergenic 40.0%
CTCTGATTATCTTTAGCGAC+AGG + chr6_4:53522778-53522797 Msa0990830:intron 40.0%
TTGCAAAACCATTGAAGCGT+AGG + chr6_4:53522972-53522991 Msa0990830:intron 40.0%
TGCTATGCTCATTCAGATAG+AGG - chr6_4:53523038-53523057 None:intergenic 40.0%
AATAGTGTTAGGAACCCAAG+AGG + chr6_4:53523259-53523278 Msa0990830:intron 40.0%
TGATATCATCAGGGTGAGTT+TGG + chr6_4:53523425-53523444 Msa0990830:intron 40.0%
! CTAGTTGTTCTTTTGTCGAC+AGG + chr6_4:53523459-53523478 Msa0990830:intron 40.0%
ACAGGAGTTGAAGTAGAGAA+AGG + chr6_4:53523477-53523496 Msa0990830:intron 40.0%
GATGATGCTTTCACCGTTAA+TGG - chr6_4:53523610-53523629 None:intergenic 40.0%
! AAAGGCATGTGTTACGTCAA+TGG + chr6_4:53523646-53523665 Msa0990830:three_prime_UTR 40.0%
TGATAGCTTGACATTGCCTT+TGG + chr6_4:53523746-53523765 Msa0990830:intron 40.0%
TTAGGCAAATCAACAACTGC+TGG + chr6_4:53523836-53523855 Msa0990830:intron 40.0%
TGTGGGAACGAGACTGAAAT+TGG - chr6_4:53520898-53520917 None:intergenic 45.0%
TACCACCCTTGTTCAATCGA+AGG + chr6_4:53520986-53521005 Msa0990830:five_prime_UTR 45.0%
ATCCTTCGATTGAACAAGGG+TGG - chr6_4:53520991-53521010 None:intergenic 45.0%
GAACTGTGGAGAAACGGAAT+GGG - chr6_4:53521061-53521080 None:intergenic 45.0%
AGAACTGTGGAGAAACGGAA+TGG - chr6_4:53521062-53521081 None:intergenic 45.0%
GGTTCCAATCAGACAAGATC+TGG + chr6_4:53521226-53521245 Msa0990830:three_prime_UTR 45.0%
CAATCAGACAAGATCTGGTC+CGG + chr6_4:53521231-53521250 Msa0990830:three_prime_UTR 45.0%
CTTCTAACCGTATCTCACAC+CGG - chr6_4:53521284-53521303 None:intergenic 45.0%
CGGTTAGAAGATCCCAACTT+CGG + chr6_4:53521294-53521313 Msa0990830:three_prime_UTR 45.0%
! TGTTTTTCCCTGTCAGTGTG+AGG + chr6_4:53521338-53521357 Msa0990830:three_prime_UTR 45.0%
! GTTTTTCCCTGTCAGTGTGA+GGG + chr6_4:53521339-53521358 Msa0990830:three_prime_UTR 45.0%
! TTGGATTAGGGTTAACGGAG+AGG + chr6_4:53521439-53521458 Msa0990830:three_prime_UTR 45.0%
AAGTTTGCCTAACAGTACCC+TGG - chr6_4:53521723-53521742 None:intergenic 45.0%
TGAATACAAGGTCGTGGCAT+TGG + chr6_4:53522066-53522085 Msa0990830:intron 45.0%
AGATTTAGCCTGTCGAGTGA+TGG + chr6_4:53522666-53522685 Msa0990830:intron 45.0%
GTAATGTTCCATCACTCGAC+AGG - chr6_4:53522677-53522696 None:intergenic 45.0%
ACTGCCAAAACGCAAGTCAT+AGG + chr6_4:53522695-53522714 Msa0990830:three_prime_UTR 45.0%
! CATACTCTGTGTCGGTACAA+AGG - chr6_4:53522805-53522824 None:intergenic 45.0%
!!! ACTTTAAAATAATATTTTAT+TGG + chr6_4:53522245-53522264 Msa0990830:intron 5.0%
!!! TTTTTAACAATATTATATTT+TGG - chr6_4:53523157-53523176 None:intergenic 5.0%
TTAGCCGCATTTGTAACCGC+CGG - chr6_4:53521205-53521224 None:intergenic 50.0%
GCGGTTACAAATGCGGCTAA+TGG + chr6_4:53521205-53521224 Msa0990830:three_prime_UTR 50.0%
CGGACCAGATCTTGTCTGAT+TGG - chr6_4:53521233-53521252 None:intergenic 50.0%
ACTGAACCCTCACACTGACA+GGG - chr6_4:53521348-53521367 None:intergenic 50.0%
AACTGAACCCTCACACTGAC+AGG - chr6_4:53521349-53521368 None:intergenic 50.0%
GTGTGAGGGTTCAGTTGAAC+TGG + chr6_4:53521353-53521372 Msa0990830:three_prime_UTR 50.0%
TGGACAACGACAACACTCGA+CGG - chr6_4:53521980-53521999 None:intergenic 50.0%
TGGTGACCTGTGAGCTCATT+TGG + chr6_4:53522086-53522105 Msa0990830:intron 50.0%
GCGGCATTGCATATTACCTC+TGG + chr6_4:53522178-53522197 Msa0990830:intron 50.0%
GACGGACATGCATGTGCAAA+AGG - chr6_4:53522617-53522636 None:intergenic 50.0%
TCAACAACTGCTGGTGTACG+TGG + chr6_4:53523845-53523864 Msa0990830:three_prime_UTR 50.0%
TTCAGCACTGCTAAGACTCG+AGG + chr6_4:53523877-53523896 Msa0990830:three_prime_UTR 50.0%
ACCTCCGGCGGTTACAAATG+CGG + chr6_4:53521198-53521217 Msa0990830:three_prime_UTR 55.0%
ACCGTATCTCACACCGGTCA+CGG - chr6_4:53521278-53521297 None:intergenic 55.0%
GCGAAGAGATCGCCGAAGTT+GGG - chr6_4:53521309-53521328 None:intergenic 55.0%
AGCGAAGAGATCGCCGAAGT+TGG - chr6_4:53521310-53521329 None:intergenic 55.0%
ATCCCGCCACGAACAACCTC+CGG + chr6_4:53521183-53521202 Msa0990830:three_prime_UTR 60.0%
TAACCGCCGGAGGTTGTTCG+TGG - chr6_4:53521192-53521211 None:intergenic 60.0%
TATCTCACACCGGTCACGGC+GGG - chr6_4:53521274-53521293 None:intergenic 60.0%
GTATCTCACACCGGTCACGG+CGG - chr6_4:53521275-53521294 None:intergenic 60.0%
GCCGTGACCGGTGTGAGATA+CGG + chr6_4:53521274-53521293 Msa0990830:three_prime_UTR 60.0%
GCCGCATTTGTAACCGCCGG+AGG - chr6_4:53521202-53521221 None:intergenic 65.0%
CGCCGGAGGTTGTTCGTGGC+GGG - chr6_4:53521188-53521207 None:intergenic 70.0%
CCGCCGGAGGTTGTTCGTGG+CGG - chr6_4:53521189-53521208 None:intergenic 70.0%
CCGCCACGAACAACCTCCGG+CGG + chr6_4:53521186-53521205 Msa0990830:three_prime_UTR 70.0%
GGACACAACGCGGAGACGGC+CGG - chr6_4:53521253-53521272 None:intergenic 70.0%
GGCGGGACACAACGCGGAGA+CGG - chr6_4:53521257-53521276 None:intergenic 70.0%
GGTCACGGCGGGACACAACG+CGG - chr6_4:53521263-53521282 None:intergenic 70.0%
GCGTTGTGTCCCGCCGTGAC+CGG + chr6_4:53521262-53521281 Msa0990830:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 53520857 53524121 53520857 ID=Msa0990830;Name=Msa0990830
chr6_4 mRNA 53520857 53524121 53520857 ID=Msa0990830-mRNA-1;Parent=Msa0990830;Name=Msa0990830-mRNA-1;_AED=0.26;_eAED=0.26;_QI=155|1|1|1|0.4|0.5|6|961|51
chr6_4 exon 53520857 53521577 53520857 ID=Msa0990830-mRNA-1:exon:11095;Parent=Msa0990830-mRNA-1
chr6_4 exon 53521958 53522032 53521958 ID=Msa0990830-mRNA-1:exon:11096;Parent=Msa0990830-mRNA-1
chr6_4 exon 53522668 53522716 53522668 ID=Msa0990830-mRNA-1:exon:11097;Parent=Msa0990830-mRNA-1
chr6_4 exon 53523481 53523547 53523481 ID=Msa0990830-mRNA-1:exon:11098;Parent=Msa0990830-mRNA-1
chr6_4 exon 53523641 53523718 53523641 ID=Msa0990830-mRNA-1:exon:11099;Parent=Msa0990830-mRNA-1
chr6_4 exon 53523840 53524121 53523840 ID=Msa0990830-mRNA-1:exon:11100;Parent=Msa0990830-mRNA-1
chr6_4 five_prime_UTR 53520857 53521011 53520857 ID=Msa0990830-mRNA-1:five_prime_utr;Parent=Msa0990830-mRNA-1
chr6_4 CDS 53521012 53521167 53521012 ID=Msa0990830-mRNA-1:cds;Parent=Msa0990830-mRNA-1
chr6_4 three_prime_UTR 53521168 53521577 53521168 ID=Msa0990830-mRNA-1:three_prime_utr;Parent=Msa0990830-mRNA-1
chr6_4 three_prime_UTR 53521958 53522032 53521958 ID=Msa0990830-mRNA-1:three_prime_utr;Parent=Msa0990830-mRNA-1
chr6_4 three_prime_UTR 53522668 53522716 53522668 ID=Msa0990830-mRNA-1:three_prime_utr;Parent=Msa0990830-mRNA-1
chr6_4 three_prime_UTR 53523481 53523547 53523481 ID=Msa0990830-mRNA-1:three_prime_utr;Parent=Msa0990830-mRNA-1
chr6_4 three_prime_UTR 53523641 53523718 53523641 ID=Msa0990830-mRNA-1:three_prime_utr;Parent=Msa0990830-mRNA-1
chr6_4 three_prime_UTR 53523840 53524121 53523840 ID=Msa0990830-mRNA-1:three_prime_utr;Parent=Msa0990830-mRNA-1
Gene Sequence

>Msa0990830

ATGTTCCTTTATCCAGTTTCATTTAAGCTCTCACCCATAATTTTAACCCATTCCGTTTCTCCACAGTTCTTCACTCTTTGTTGTTCTTCAAATCCTTTTCGACCCATTTCTTTTCTACAGTTGCTCAGTTCCTTTTCTTCCAAAACATATATATAG
Protein sequence

>Msa0990830

MFLYPVSFKLSPIILTHSVSPQFFTLCCSSNPFRPISFLQLLSSFSSKTYI*