Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1298310 | AES93751.1 | 73.494 | 83 | 10 | 1 | 1 | 71 | 15 | 97 | 5.74e-31 | 114 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1298310 | sp|Q9SSF0|HIP16_ARATH | 46.377 | 69 | 37 | 0 | 1 | 69 | 11 | 79 | 1.49e-14 | 66.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1298310 | G7K532 | 73.494 | 83 | 10 | 1 | 1 | 71 | 15 | 97 | 2.74e-31 | 114 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0023090 | Msa1298310 | 0.801995 | 6.921130e-49 | -8.615850e-47 |
| Msa0657970 | Msa1298310 | 0.804122 | 2.514135e-49 | -8.615850e-47 |
| Msa0693380 | Msa1298310 | 0.809445 | 1.885501e-50 | -8.615850e-47 |
| Msa0742120 | Msa1298310 | 0.901173 | 3.501698e-78 | -8.615850e-47 |
| Msa0766630 | Msa1298310 | 0.808028 | 3.787786e-50 | -8.615850e-47 |
| Msa0822650 | Msa1298310 | 0.805543 | 1.268971e-49 | -8.615850e-47 |
| Msa0233300 | Msa1298310 | 0.806657 | 7.395048e-50 | -8.615850e-47 |
| Msa1279170 | Msa1298310 | 0.895260 | 1.134336e-75 | -8.615850e-47 |
| Msa1298310 | Msa1434350 | 0.802256 | 6.117056e-49 | -8.615850e-47 |
| Msa1298310 | Msa1439320 | 0.800821 | 1.204183e-48 | -8.615850e-47 |
| Msa1082500 | Msa1298310 | 0.822012 | 2.973645e-53 | -8.615850e-47 |
| Msa0339620 | Msa1298310 | 0.800150 | 1.649700e-48 | -8.615850e-47 |
| Msa0484780 | Msa1298310 | 0.810979 | 8.805447e-51 | -8.615850e-47 |
| Msa0532350 | Msa1298310 | 0.804198 | 2.423292e-49 | -8.615850e-47 |
| Msa0907070 | Msa1298310 | 0.801333 | 9.461230e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1298310 | MtrunA17_Chr3g0120541 | 80.000 | 70 | 14 | 0 | 1 | 70 | 12 | 81 | 6.43e-35 | 114 |
| Msa1298310 | MtrunA17_Chr3g0120531 | 82.258 | 62 | 11 | 0 | 1 | 62 | 13 | 74 | 1.02e-32 | 108 |
| Msa1298310 | MtrunA17_Chr3g0120551 | 69.697 | 66 | 20 | 0 | 1 | 66 | 12 | 77 | 1.55e-26 | 93.2 |
| Msa1298310 | MtrunA17_Chr2g0290831 | 43.077 | 65 | 37 | 0 | 1 | 65 | 11 | 75 | 6.75e-14 | 60.1 |
| Msa1298310 | MtrunA17_Chr5g0438551 | 42.683 | 82 | 36 | 2 | 1 | 71 | 10 | 91 | 4.08e-13 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1298310 | AT3G07600.1 | 46.377 | 69 | 37 | 0 | 1 | 69 | 11 | 79 | 1.52e-15 | 66.6 |
| Msa1298310 | AT5G48290.2 | 49.296 | 71 | 34 | 1 | 1 | 69 | 1 | 71 | 5.69e-15 | 65.5 |
| Msa1298310 | AT5G48290.1 | 49.296 | 71 | 34 | 1 | 1 | 69 | 11 | 81 | 6.45e-15 | 65.5 |
| Msa1298310 | AT3G20180.1 | 39.706 | 68 | 41 | 0 | 1 | 68 | 7 | 74 | 2.22e-12 | 57.8 |
| Msa1298310 | AT3G20180.4 | 39.706 | 68 | 41 | 0 | 1 | 68 | 9 | 76 | 2.34e-12 | 57.8 |
| Msa1298310 | AT3G20180.3 | 39.706 | 68 | 41 | 0 | 1 | 68 | 11 | 78 | 2.55e-12 | 57.8 |
| Msa1298310 | AT3G20180.2 | 41.096 | 73 | 41 | 1 | 1 | 71 | 30 | 102 | 2.89e-12 | 57.8 |
| Msa1298310 | AT5G48290.3 | 39.560 | 91 | 33 | 2 | 1 | 69 | 11 | 101 | 2.06e-11 | 56.6 |
Find 14 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTATATCTCATAAGTATTT+TGG | 0.150399 | 8_3:-59117096 | Msa1298310:five_prime_UTR |
| AAGATTGCAGTTGGAGTTTC+AGG | 0.157536 | 8_3:-59116617 | Msa1298310:intron |
| ACTTATGAGATATAAATAAA+TGG | 0.288883 | 8_3:+59117103 | None:intergenic |
| AACTGCAATCTTCATGGCTT+TGG | 0.309449 | 8_3:+59116628 | None:intergenic |
| AAAATTATGTCATGCAGAAT+TGG | 0.351294 | 8_3:-59116426 | Msa1298310:CDS |
| AACTCCAACTGCAATCTTCA+TGG | 0.358381 | 8_3:+59116622 | None:intergenic |
| AAGGATCAAATAGAGATAAC+AGG | 0.396822 | 8_3:-59116491 | Msa1298310:CDS |
| ATTGGTGAGTGTTGGTGAAG+TGG | 0.534161 | 8_3:-59116408 | Msa1298310:CDS |
| GCAGCAAAAGATAGTGATCA+AGG | 0.536634 | 8_3:-59116678 | Msa1298310:intron |
| TGGTGAAGTGGAAAAGAAAG+AGG | 0.582213 | 8_3:-59116396 | Msa1298310:CDS |
| AAAGCCATGAAGATTGCAGT+TGG | 0.583776 | 8_3:-59116626 | Msa1298310:CDS |
| TCAAATAATCACATATAGCA+CGG | 0.588637 | 8_3:-59117145 | Msa1298310:five_prime_UTR |
| AGCTATAAAAGCAGATAGCA+AGG | 0.594508 | 8_3:-59116510 | Msa1298310:CDS |
| CATGCAGAATTGGTGAGTGT+TGG | 0.631342 | 8_3:-59116416 | Msa1298310:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTTATGAGATATAAATAAA+TGG | + | chr8_3:59116427-59116446 | None:intergenic | 15.0% |
| !!! | TTTATATCTCATAAGTATTT+TGG | - | chr8_3:59116431-59116450 | Msa1298310:CDS | 15.0% |
| !!! | CTATATATTTTAAATAAAGG+AGG | + | chr8_3:59116497-59116516 | None:intergenic | 15.0% |
| !! | ACTTATGAGATATAAATAAA+TGG | + | chr8_3:59116427-59116446 | None:intergenic | 15.0% |
| !!! | TTTATATCTCATAAGTATTT+TGG | - | chr8_3:59116431-59116450 | Msa1298310:CDS | 15.0% |
| !!! | CTATATATTTTAAATAAAGG+AGG | + | chr8_3:59116497-59116516 | None:intergenic | 15.0% |
| ! | AGCAAAGGATATATAATAGA+AGG | + | chr8_3:59116565-59116584 | None:intergenic | 25.0% |
| !! | AATCTTTTCTTCGAAAGTTT+TGG | + | chr8_3:59116707-59116726 | None:intergenic | 25.0% |
| ! | TTTGGACTTTGGATAATTAA+TGG | + | chr8_3:59116960-59116979 | None:intergenic | 25.0% |
| ! | AAAATTATGTCATGCAGAAT+TGG | - | chr8_3:59117101-59117120 | Msa1298310:five_prime_UTR | 25.0% |
| ! | AGCAAAGGATATATAATAGA+AGG | + | chr8_3:59116565-59116584 | None:intergenic | 25.0% |
| !! | AATCTTTTCTTCGAAAGTTT+TGG | + | chr8_3:59116707-59116726 | None:intergenic | 25.0% |
| ! | TTTGGACTTTGGATAATTAA+TGG | + | chr8_3:59116960-59116979 | None:intergenic | 25.0% |
| ! | AAAATTATGTCATGCAGAAT+TGG | - | chr8_3:59117101-59117120 | Msa1298310:five_prime_UTR | 25.0% |
| !!! | TCTCATAAGTATTTTGGCTA+AGG | - | chr8_3:59116437-59116456 | Msa1298310:CDS | 30.0% |
| AAAATTGAGAGGGAAAATGT+AGG | - | chr8_3:59116587-59116606 | Msa1298310:intron | 30.0% | |
| AAATTGAGAGGGAAAATGTA+GGG | - | chr8_3:59116588-59116607 | Msa1298310:intron | 30.0% | |
| CAATGAACAACCAAAAATCA+AGG | - | chr8_3:59116875-59116894 | Msa1298310:intron | 30.0% | |
| ! | ATTTCTGCACATGATTAGTT+TGG | + | chr8_3:59116978-59116997 | None:intergenic | 30.0% |
| AATCATGTGCAGAAATATTC+AGG | - | chr8_3:59116982-59117001 | Msa1298310:intron | 30.0% | |
| AAGGATCAAATAGAGATAAC+AGG | - | chr8_3:59117036-59117055 | Msa1298310:intron | 30.0% | |
| !!! | TCTCATAAGTATTTTGGCTA+AGG | - | chr8_3:59116437-59116456 | Msa1298310:CDS | 30.0% |
| AAAATTGAGAGGGAAAATGT+AGG | - | chr8_3:59116587-59116606 | Msa1298310:intron | 30.0% | |
| AAATTGAGAGGGAAAATGTA+GGG | - | chr8_3:59116588-59116607 | Msa1298310:intron | 30.0% | |
| CAATGAACAACCAAAAATCA+AGG | - | chr8_3:59116875-59116894 | Msa1298310:intron | 30.0% | |
| ! | ATTTCTGCACATGATTAGTT+TGG | + | chr8_3:59116978-59116997 | None:intergenic | 30.0% |
| AATCATGTGCAGAAATATTC+AGG | - | chr8_3:59116982-59117001 | Msa1298310:intron | 30.0% | |
| AAGGATCAAATAGAGATAAC+AGG | - | chr8_3:59117036-59117055 | Msa1298310:intron | 30.0% | |
| TCCTTTGCTTGAAAATTGAG+AGG | - | chr8_3:59116576-59116595 | Msa1298310:intron | 35.0% | |
| CCTTTGCTTGAAAATTGAGA+GGG | - | chr8_3:59116577-59116596 | Msa1298310:intron | 35.0% | |
| !! | CTTTTCTTCGAAAGTTTTGG+TGG | + | chr8_3:59116704-59116723 | None:intergenic | 35.0% |
| !! | CACATGATTAGTTTGGACTT+TGG | + | chr8_3:59116971-59116990 | None:intergenic | 35.0% |
| AGCTATAAAAGCAGATAGCA+AGG | - | chr8_3:59117017-59117036 | Msa1298310:intron | 35.0% | |
| TCCTTTGCTTGAAAATTGAG+AGG | - | chr8_3:59116576-59116595 | Msa1298310:intron | 35.0% | |
| CCTTTGCTTGAAAATTGAGA+GGG | - | chr8_3:59116577-59116596 | Msa1298310:intron | 35.0% | |
| !! | CTTTTCTTCGAAAGTTTTGG+TGG | + | chr8_3:59116704-59116723 | None:intergenic | 35.0% |
| !! | CACATGATTAGTTTGGACTT+TGG | + | chr8_3:59116971-59116990 | None:intergenic | 35.0% |
| AGCTATAAAAGCAGATAGCA+AGG | - | chr8_3:59117017-59117036 | Msa1298310:intron | 35.0% | |
| !! | CCCTCTCAATTTTCAAGCAA+AGG | + | chr8_3:59116580-59116599 | None:intergenic | 40.0% |
| AGGGAAAATGTAGGGAAAGA+GGG | - | chr8_3:59116596-59116615 | Msa1298310:intron | 40.0% | |
| GCAGCAAAAGATAGTGATCA+AGG | - | chr8_3:59116849-59116868 | Msa1298310:intron | 40.0% | |
| !!! | TGGCTTTGGACCTTGATTTT+TGG | + | chr8_3:59116888-59116907 | None:intergenic | 40.0% |
| AACTGCAATCTTCATGGCTT+TGG | + | chr8_3:59116902-59116921 | None:intergenic | 40.0% | |
| AAAGCCATGAAGATTGCAGT+TGG | - | chr8_3:59116901-59116920 | Msa1298310:intron | 40.0% | |
| AACTCCAACTGCAATCTTCA+TGG | + | chr8_3:59116908-59116927 | None:intergenic | 40.0% | |
| AAGATTGCAGTTGGAGTTTC+AGG | - | chr8_3:59116910-59116929 | Msa1298310:intron | 40.0% | |
| TGTGCAGAAATATTCAGGAG+TGG | - | chr8_3:59116987-59117006 | Msa1298310:intron | 40.0% | |
| TGGTGAAGTGGAAAAGAAAG+AGG | - | chr8_3:59117131-59117150 | Msa1298310:five_prime_UTR | 40.0% | |
| !! | CCCTCTCAATTTTCAAGCAA+AGG | + | chr8_3:59116580-59116599 | None:intergenic | 40.0% |
| AGGGAAAATGTAGGGAAAGA+GGG | - | chr8_3:59116596-59116615 | Msa1298310:intron | 40.0% | |
| GCAGCAAAAGATAGTGATCA+AGG | - | chr8_3:59116849-59116868 | Msa1298310:intron | 40.0% | |
| !!! | TGGCTTTGGACCTTGATTTT+TGG | + | chr8_3:59116888-59116907 | None:intergenic | 40.0% |
| AACTGCAATCTTCATGGCTT+TGG | + | chr8_3:59116902-59116921 | None:intergenic | 40.0% | |
| AAAGCCATGAAGATTGCAGT+TGG | - | chr8_3:59116901-59116920 | Msa1298310:intron | 40.0% | |
| AACTCCAACTGCAATCTTCA+TGG | + | chr8_3:59116908-59116927 | None:intergenic | 40.0% | |
| AAGATTGCAGTTGGAGTTTC+AGG | - | chr8_3:59116910-59116929 | Msa1298310:intron | 40.0% | |
| TGTGCAGAAATATTCAGGAG+TGG | - | chr8_3:59116987-59117006 | Msa1298310:intron | 40.0% | |
| TGGTGAAGTGGAAAAGAAAG+AGG | - | chr8_3:59117131-59117150 | Msa1298310:five_prime_UTR | 40.0% | |
| AGGAGGTAACACTACTTAGC+AGG | + | chr8_3:59116480-59116499 | None:intergenic | 45.0% | |
| GAGGGAAAATGTAGGGAAAG+AGG | - | chr8_3:59116595-59116614 | Msa1298310:intron | 45.0% | |
| GACTTGATGAGCTAGAGAGT+TGG | - | chr8_3:59116774-59116793 | Msa1298310:intron | 45.0% | |
| CATGCAGAATTGGTGAGTGT+TGG | - | chr8_3:59117111-59117130 | Msa1298310:five_prime_UTR | 45.0% | |
| !! | ATTGGTGAGTGTTGGTGAAG+TGG | - | chr8_3:59117119-59117138 | Msa1298310:five_prime_UTR | 45.0% |
| AGGAGGTAACACTACTTAGC+AGG | + | chr8_3:59116480-59116499 | None:intergenic | 45.0% | |
| GAGGGAAAATGTAGGGAAAG+AGG | - | chr8_3:59116595-59116614 | Msa1298310:intron | 45.0% | |
| GACTTGATGAGCTAGAGAGT+TGG | - | chr8_3:59116774-59116793 | Msa1298310:intron | 45.0% | |
| CATGCAGAATTGGTGAGTGT+TGG | - | chr8_3:59117111-59117130 | Msa1298310:five_prime_UTR | 45.0% | |
| !! | ATTGGTGAGTGTTGGTGAAG+TGG | - | chr8_3:59117119-59117138 | Msa1298310:five_prime_UTR | 45.0% |
| !!! | TAACTATATATTTTAAATAA+AGG | + | chr8_3:59116500-59116519 | None:intergenic | 5.0% |
| !!! | TAACTATATATTTTAAATAA+AGG | + | chr8_3:59116500-59116519 | None:intergenic | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 59116385 | 59117164 | 59116385 | ID=Msa1298310;Name=Msa1298310 |
| chr8_3 | mRNA | 59116385 | 59117164 | 59116385 | ID=Msa1298310-mRNA-1;Parent=Msa1298310;Name=Msa1298310-mRNA-1;_AED=0.10;_eAED=0.10;_QI=98|1|0.66|1|0|0|3|0|71 |
| chr8_3 | exon | 59116385 | 59116545 | 59116385 | ID=Msa1298310-mRNA-1:exon:17062;Parent=Msa1298310-mRNA-1 |
| chr8_3 | exon | 59116618 | 59116696 | 59116618 | ID=Msa1298310-mRNA-1:exon:17061;Parent=Msa1298310-mRNA-1 |
| chr8_3 | exon | 59117091 | 59117164 | 59117091 | ID=Msa1298310-mRNA-1:exon:17060;Parent=Msa1298310-mRNA-1 |
| chr8_3 | five_prime_UTR | 59117091 | 59117164 | 59117091 | ID=Msa1298310-mRNA-1:five_prime_utr;Parent=Msa1298310-mRNA-1 |
| chr8_3 | five_prime_UTR | 59116673 | 59116696 | 59116673 | ID=Msa1298310-mRNA-1:five_prime_utr;Parent=Msa1298310-mRNA-1 |
| chr8_3 | CDS | 59116618 | 59116672 | 59116618 | ID=Msa1298310-mRNA-1:cds;Parent=Msa1298310-mRNA-1 |
| chr8_3 | CDS | 59116385 | 59116545 | 59116385 | ID=Msa1298310-mRNA-1:cds;Parent=Msa1298310-mRNA-1 |
| Gene Sequence |
| Protein sequence |
