AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1303090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1303090 MtrunA17_Chr4g0074021 100.000 159 0 0 1 159 1 159 5.08e-117 327
Msa1303090 MtrunA17_Chr4g0061171 93.711 159 10 0 1 159 1 159 2.71e-109 308
Msa1303090 MtrunA17_Chr3g0091481 92.453 159 12 0 1 159 1 159 1.42e-108 306
Msa1303090 MtrunA17_Chr5g0418211 89.937 159 16 0 1 159 1 159 1.75e-106 300
Msa1303090 MtrunA17_Chr4g0062241 68.627 102 22 1 1 102 1 92 3.17e-45 143
Msa1303090 MtrunA17_Chr4g0074011 47.656 128 19 2 18 145 42 121 1.06e-26 98.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1303090 AT1G13950.1 91.824 159 12 1 1 159 1 158 4.28e-105 297
Msa1303090 AT1G69410.1 86.792 159 20 1 1 159 1 158 9.26e-102 289
Msa1303090 AT1G26630.1 81.529 157 28 1 1 157 1 156 1.42e-93 268
Msa1303090 AT1G26630.2 84.252 127 19 1 1 127 1 126 6.93e-76 223

Find 64 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATATCCAATAAGTTATTT+TGG 0.141811 8_3:+65867172 Msa1303090:three_prime_UTR
TTCATCCCAAAATAACTTAT+TGG 0.178416 8_3:-65867178 None:intergenic
GATCAATGCGGTTAACTTAT+AGG 0.258167 8_3:+65866959 Msa1303090:three_prime_UTR
ATTATTGAACTCTCTGGTTT+TGG 0.270929 8_3:+65866711 Msa1303090:three_prime_UTR
ATCAATGCGGTTAACTTATA+GGG 0.274474 8_3:+65866960 Msa1303090:three_prime_UTR
CGGTCGATTAGTTGTGTCTA+AGG 0.307333 8_3:+65867067 Msa1303090:three_prime_UTR
AAAGAGCACAGAGGAGAGAT+AGG 0.326363 8_3:-65866795 None:intergenic
AGCCTGTTGAGGGTAGGTTT+TGG 0.330390 8_3:-65864394 None:intergenic
AGGAAGCTTCAGATCATCTT+TGG 0.330486 8_3:-65866369 None:intergenic
ACTTAGGACCAATGTCCTTC+AGG 0.343359 8_3:-65866578 None:intergenic
TTGATTGATATTTCTGAAGA+TGG 0.371340 8_3:+65865496 Msa1303090:CDS
CTTATAGGGGATGCGGATCT+AGG 0.397089 8_3:+65866974 Msa1303090:three_prime_UTR
TCTTTAATTATTGAACTCTC+TGG 0.403510 8_3:+65866705 Msa1303090:three_prime_UTR
CTGAGTAGTTGTGTATATAT+TGG 0.412034 8_3:+65867043 Msa1303090:three_prime_UTR
TGAGGATCGGATGAATGATT+AGG 0.418550 8_3:+65864206 None:intergenic
GCAGAAAACCTTGCAAGGCC+TGG 0.423629 8_3:-65864454 None:intergenic
TTAAAGAGACAAGATAAATA+TGG 0.437632 8_3:-65866689 None:intergenic
ATCCAAAACCTACCCTCAAC+AGG 0.440341 8_3:+65864392 Msa1303090:CDS
ATACATTCATAGATTAAAGA+TGG 0.442913 8_3:+65866489 Msa1303090:intron
TAAAACATGGTGTGGAGAAA+AGG 0.446845 8_3:+65866665 Msa1303090:three_prime_UTR
GGATGCTCCGGCATCGGCAG+CGG 0.446886 8_3:-65864373 None:intergenic
GATGCTCCGGCATCGGCAGC+GGG 0.447940 8_3:-65864372 None:intergenic
TTTGAACCCGCTGCCGATGC+CGG 0.456716 8_3:+65864366 Msa1303090:CDS
TTAGTTGTGTCTAAGGGTTA+AGG 0.475769 8_3:+65867074 Msa1303090:three_prime_UTR
CTTAGGACCAATGTCCTTCA+GGG 0.484561 8_3:-65866577 None:intergenic
ATTAAAGATGGATTTGCTGA+TGG 0.488295 8_3:+65866501 Msa1303090:CDS
GGACACTTAAAGAATTCATC+TGG 0.489024 8_3:-65866734 None:intergenic
GAATAGTACCAGCCTGTTGA+GGG 0.502881 8_3:-65864404 None:intergenic
GGTTTCTGTTATGTCTGCGA+TGG 0.503255 8_3:+65866536 Msa1303090:CDS
ACATCACAATTGTGGGATGA+AGG 0.511953 8_3:-65865366 None:intergenic
CTAACTTACATCACAATTGT+GGG 0.513530 8_3:-65865373 None:intergenic
TCTAACTTACATCACAATTG+TGG 0.519354 8_3:-65865374 None:intergenic
CATCAAGTCCAGGCCTTGCA+AGG 0.525229 8_3:+65864446 Msa1303090:CDS
GCTGGTACTATTCGCAAGAA+TGG 0.527051 8_3:+65864414 Msa1303090:CDS
CGAATAGTACCAGCCTGTTG+AGG 0.540601 8_3:-65864405 None:intergenic
TTGATTGCAAGTTATAAAGC+TGG 0.545199 8_3:-65866915 None:intergenic
CGGTTAACTTATAGGGGATG+CGG 0.553112 8_3:+65866967 Msa1303090:three_prime_UTR
GTTTCTGTTATGTCTGCGAT+GGG 0.555762 8_3:+65866537 Msa1303090:CDS
GTGTGTGATGTAGTGTGCAC+TGG 0.568452 8_3:+65866855 Msa1303090:three_prime_UTR
TTGCAAGTTATAAAGCTGGT+TGG 0.569207 8_3:-65866911 None:intergenic
AAGAGCACAGAGGAGAGATA+GGG 0.569296 8_3:-65866794 None:intergenic
GGTCGATTAGTTGTGTCTAA+GGG 0.576561 8_3:+65867068 Msa1303090:three_prime_UTR
AAAACCTACCCTCAACAGGC+TGG 0.584266 8_3:+65864396 Msa1303090:CDS
GATGCTTTCTCTGCGCGCGT+GGG 0.584738 8_3:-65864314 None:intergenic
GAAACTCATAAAACATGGTG+TGG 0.589277 8_3:+65866657 Msa1303090:three_prime_UTR
AGAGCAGATATGTGCCCTGA+AGG 0.589389 8_3:+65866563 Msa1303090:CDS
TGCTGATGGAAAAGATCTCG+TGG 0.593109 8_3:+65866515 Msa1303090:CDS
GAAAAGCAGAAAACCTTGCA+AGG 0.593565 8_3:-65864459 None:intergenic
ATATGTGCCCTGAAGGACAT+TGG 0.593679 8_3:+65866570 Msa1303090:CDS
GTTACATAGTCATCAAGTCC+AGG 0.598114 8_3:+65864436 Msa1303090:CDS
TGATGCTTTCTCTGCGCGCG+TGG 0.598798 8_3:-65864315 None:intergenic
TGATGATTCACTACTTACTC+AGG 0.602143 8_3:+65866394 Msa1303090:CDS
TCAATGCGGTTAACTTATAG+GGG 0.604359 8_3:+65866961 Msa1303090:three_prime_UTR
GTAAGTAGTGAATCATCAGT+AGG 0.605999 8_3:-65866389 None:intergenic
AGTACCAGCCTGTTGAGGGT+AGG 0.611010 8_3:-65864400 None:intergenic
GTGAGTTTGTTGACTGACAA+TGG 0.614472 8_3:+65866341 Msa1303090:CDS
TATGTGTGAGGAGAAGAAGA+TGG 0.621382 8_3:-65864283 None:intergenic
GTAGTTGTGTATATATTGGA+CGG 0.637381 8_3:+65867047 Msa1303090:three_prime_UTR
GCTCAATAAACTTGTCAATG+TGG 0.641522 8_3:+65866624 Msa1303090:three_prime_UTR
AGCTTGAAACTCATAAAACA+TGG 0.668434 8_3:+65866652 Msa1303090:three_prime_UTR
TTTATAACTTGCAATCAACA+CGG 0.675149 8_3:+65866920 Msa1303090:three_prime_UTR
TGGGTAATGTGTTATGTGTG+AGG 0.693706 8_3:-65864295 None:intergenic
TAATCAGTACGATTCACATG+AGG 0.718954 8_3:-65865469 None:intergenic
ACTAGTTGAAAAGAGCACAG+AGG 0.749892 8_3:-65866804 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGATTTGATTGTTTTAAA+CGG + chr8_3:65864694-65864713 Msa1303090:intron 15.0%
!!! TTTGAAACAATTTTTAAAGA+TGG + chr8_3:65865025-65865044 Msa1303090:intron 15.0%
!!! TTTTATAAACTTGATATAAC+TGG + chr8_3:65865087-65865106 Msa1303090:intron 15.0%
!! AATATATTGAGAAACAAATA+CGG - chr8_3:65865237-65865256 None:intergenic 15.0%
!!! ATTTGTTTCTCAATATATTT+TGG + chr8_3:65865238-65865257 Msa1303090:intron 15.0%
!! ATTAAGTTAAAACTTTCAAA+TGG + chr8_3:65865566-65865585 Msa1303090:intron 15.0%
!! ACAAAAATATAAAGTTACAA+GGG - chr8_3:65866758-65866777 None:intergenic 15.0%
!! AACAAAAATATAAAGTTACA+AGG - chr8_3:65866759-65866778 None:intergenic 15.0%
!!! ATATATCCAATAAGTTATTT+TGG + chr8_3:65867172-65867191 Msa1303090:three_prime_UTR 15.0%
!!! TTTAGAATAAAAAGAGAGTT+AGG + chr8_3:65864229-65864248 Msa1303090:five_prime_UTR 20.0%
!! TTAGAATAAAAAGAGAGTTA+GGG + chr8_3:65864230-65864249 Msa1303090:five_prime_UTR 20.0%
!! AAAAAATACAAATCTAGAAG+GGG - chr8_3:65864490-65864509 None:intergenic 20.0%
!! CAAAAAATACAAATCTAGAA+GGG - chr8_3:65864491-65864510 None:intergenic 20.0%
!! TCAAAAAATACAAATCTAGA+AGG - chr8_3:65864492-65864511 None:intergenic 20.0%
!! TGTTTGAATCGAATAAAAAT+TGG + chr8_3:65864650-65864669 Msa1303090:intron 20.0%
!! ATAAAAATTGGAACATGATT+TGG + chr8_3:65864662-65864681 Msa1303090:intron 20.0%
!! AAAAATTGTTTCAAAACCTA+GGG - chr8_3:65865020-65865039 None:intergenic 20.0%
!! TAAAAATTGTTTCAAAACCT+AGG - chr8_3:65865021-65865040 None:intergenic 20.0%
!!! TTGCTATTGATATTTTCAAT+GGG + chr8_3:65865322-65865341 Msa1303090:CDS 20.0%
!! GGATATATAAAATGAGAATT+AGG + chr8_3:65865587-65865606 Msa1303090:intron 20.0%
!! TGTTATCTTAATGTCTATTA+AGG + chr8_3:65865637-65865656 Msa1303090:intron 20.0%
!! GTTATCTTAATGTCTATTAA+GGG + chr8_3:65865638-65865657 Msa1303090:intron 20.0%
!!! ATAAAACATACTCAGTTTTT+AGG - chr8_3:65865682-65865701 None:intergenic 20.0%
!! AAATATTATAGTCATTCCTT+TGG - chr8_3:65865789-65865808 None:intergenic 20.0%
!!! TTTACATTATGTTTTATGTC+AGG + chr8_3:65865820-65865839 Msa1303090:intron 20.0%
!! TTTAGTGTAATTATAAGTGT+CGG + chr8_3:65866017-65866036 Msa1303090:intron 20.0%
!! AAGTAAAAATAAGATGACAT+AGG + chr8_3:65866122-65866141 Msa1303090:intron 20.0%
!! AGTAAAAATAAGATGACATA+GGG + chr8_3:65866123-65866142 Msa1303090:intron 20.0%
!! AAAGAGACAAAATATATATG+TGG - chr8_3:65866454-65866473 None:intergenic 20.0%
!!! GTCTCTTTAATTTGATTTAT+TGG + chr8_3:65866466-65866485 Msa1303090:intron 20.0%
!! ATACATTCATAGATTAAAGA+TGG + chr8_3:65866489-65866508 Msa1303090:intron 20.0%
!! TTAAAGAGACAAGATAAATA+TGG - chr8_3:65866692-65866711 None:intergenic 20.0%
!! TGATGGAGAAAAATAAATTT+AGG + chr8_3:65866827-65866846 Msa1303090:three_prime_UTR 20.0%
! AATTCCTTATTCGATTAATC+TGG + chr8_3:65864511-65864530 Msa1303090:intron 25.0%
! TTAACATGCTTCTGTTTATA+TGG + chr8_3:65864597-65864616 Msa1303090:intron 25.0%
!!! TGTTTATATGGTGTTGATTT+TGG + chr8_3:65864609-65864628 Msa1303090:intron 25.0%
! TTTACATCTAGATCTAACAT+TGG + chr8_3:65864739-65864758 Msa1303090:intron 25.0%
!! AAGTTAATCTCTTTTCAACA+TGG - chr8_3:65864798-65864817 None:intergenic 25.0%
!!! AATTGTGCTAACTTGTTTAT+TGG + chr8_3:65865208-65865227 Msa1303090:intron 25.0%
!!! ATTGTGCTAACTTGTTTATT+GGG + chr8_3:65865209-65865228 Msa1303090:intron 25.0%
!!! GTTTCTCAATATATTTTGGT+AGG + chr8_3:65865242-65865261 Msa1303090:intron 25.0%
!!! GTTGCTATTGATATTTTCAA+TGG + chr8_3:65865321-65865340 Msa1303090:CDS 25.0%
!! TTGATTGATATTTCTGAAGA+TGG + chr8_3:65865496-65865515 Msa1303090:CDS 25.0%
! GTTATATAGATGAGTTACAT+GGG + chr8_3:65865609-65865628 Msa1303090:intron 25.0%
! TTATATAGATGAGTTACATG+GGG + chr8_3:65865610-65865629 Msa1303090:intron 25.0%
!! TTCACAGTAAATTTTACCAA+AGG + chr8_3:65865770-65865789 Msa1303090:intron 25.0%
!! ACTTGTTTGTATTCACATTT+TGG - chr8_3:65866238-65866257 None:intergenic 25.0%
! CATACGAAAAATTACTACTT+AGG - chr8_3:65866597-65866616 None:intergenic 25.0%
! TCTTTAATTATTGAACTCTC+TGG + chr8_3:65866705-65866724 Msa1303090:three_prime_UTR 25.0%
!! TTTATAACTTGCAATCAACA+CGG + chr8_3:65866920-65866939 Msa1303090:three_prime_UTR 25.0%
!!! AGTTAAGAGATTTTTTGGTG+CGG - chr8_3:65864260-65864279 None:intergenic 30.0%
!!! GGATGAGTTAAGAGATTTTT+TGG - chr8_3:65864265-65864284 None:intergenic 30.0%
GATTCCAGATTAATCGAATA+AGG - chr8_3:65864518-65864537 None:intergenic 30.0%
!!! ATTTTGGTTGATTGGTAACT+GGG + chr8_3:65864625-65864644 Msa1303090:intron 30.0%
!!! CATTGGAATTTTAGTTTTGC+TGG + chr8_3:65865153-65865172 Msa1303090:intron 30.0%
!!! TATATTTTGGTAGGTTGTTG+AGG + chr8_3:65865251-65865270 Msa1303090:intron 30.0%
ACTTCAAAAACAGGAAAACA+TGG + chr8_3:65865279-65865298 Msa1303090:CDS 30.0%
CTAACTTACATCACAATTGT+GGG - chr8_3:65865376-65865395 None:intergenic 30.0%
TCTAACTTACATCACAATTG+TGG - chr8_3:65865377-65865396 None:intergenic 30.0%
TTACTTGTGACAACTTTAGT+AGG + chr8_3:65865537-65865556 Msa1303090:intron 30.0%
GGTTATATAGATGAGTTACA+TGG + chr8_3:65865608-65865627 Msa1303090:intron 30.0%
TATATAGATGAGTTACATGG+GGG + chr8_3:65865611-65865630 Msa1303090:intron 30.0%
TTAATGTCTATTAAGGGATG+AGG + chr8_3:65865644-65865663 Msa1303090:intron 30.0%
TAATGTCTATTAAGGGATGA+GGG + chr8_3:65865645-65865664 Msa1303090:intron 30.0%
TTCTTCATGTTAGGAAAACA+AGG - chr8_3:65865740-65865759 None:intergenic 30.0%
TAGTTGACATTCTTCATGTT+AGG - chr8_3:65865749-65865768 None:intergenic 30.0%
TTCTTCATGTCAGGAAAATA+TGG - chr8_3:65865875-65865894 None:intergenic 30.0%
! TATTTGACTTTCTTCATGTC+AGG - chr8_3:65865884-65865903 None:intergenic 30.0%
CCTAAACAAAACATTCATGT+AGG - chr8_3:65866322-65866341 None:intergenic 30.0%
!!! CCTACATGAATGTTTTGTTT+AGG + chr8_3:65866319-65866338 Msa1303090:intron 30.0%
!! ATTAAAGATGGATTTGCTGA+TGG + chr8_3:65866501-65866520 Msa1303090:CDS 30.0%
AGCTTGAAACTCATAAAACA+TGG + chr8_3:65866652-65866671 Msa1303090:three_prime_UTR 30.0%
!! ATTATTGAACTCTCTGGTTT+TGG + chr8_3:65866711-65866730 Msa1303090:three_prime_UTR 30.0%
TTGATTGCAAGTTATAAAGC+TGG - chr8_3:65866918-65866937 None:intergenic 30.0%
!!! ACACGGTTAACTTTTTTCAA+AGG + chr8_3:65866937-65866956 Msa1303090:three_prime_UTR 30.0%
!!! CTTTTTTCAAAGGATCAATG+CGG + chr8_3:65866947-65866966 Msa1303090:three_prime_UTR 30.0%
ATCAATGCGGTTAACTTATA+GGG + chr8_3:65866960-65866979 Msa1303090:three_prime_UTR 30.0%
CTGAGTAGTTGTGTATATAT+TGG + chr8_3:65867043-65867062 Msa1303090:three_prime_UTR 30.0%
GTAGTTGTGTATATATTGGA+CGG + chr8_3:65867047-65867066 Msa1303090:three_prime_UTR 30.0%
TACAAATCTAGAAGGGGAAA+GGG - chr8_3:65864484-65864503 None:intergenic 35.0%
ATACAAATCTAGAAGGGGAA+AGG - chr8_3:65864485-65864504 None:intergenic 35.0%
!!! TGGTGTTGATTTTGGTTGAT+TGG + chr8_3:65864617-65864636 Msa1303090:intron 35.0%
!!! GATTTTGGTTGATTGGTAAC+TGG + chr8_3:65864624-65864643 Msa1303090:intron 35.0%
TAAACGGAGACATAGACATA+TGG + chr8_3:65864710-65864729 Msa1303090:intron 35.0%
CTCAATAAGTCAATCCAAAC+AGG + chr8_3:65864994-65865013 Msa1303090:intron 35.0%
GAGGTTTCTACTTCAAAAAC+AGG + chr8_3:65865270-65865289 Msa1303090:CDS 35.0%
TAATCAGTACGATTCACATG+AGG - chr8_3:65865472-65865491 None:intergenic 35.0%
TTGTGACAACTTTAGTAGGT+GGG + chr8_3:65865541-65865560 Msa1303090:intron 35.0%
!!! CATACTCAGTTTTTAGGACA+TGG - chr8_3:65865676-65865695 None:intergenic 35.0%
GAGTTTACAACTATGAAGCA+TGG - chr8_3:65866067-65866086 None:intergenic 35.0%
ACAAGTGCACAACAAGAAAA+AGG + chr8_3:65866252-65866271 Msa1303090:intron 35.0%
CAAGTGCACAACAAGAAAAA+GGG + chr8_3:65866253-65866272 Msa1303090:intron 35.0%
TTATGCTATTGTCATTGCAG+TGG + chr8_3:65866284-65866303 Msa1303090:intron 35.0%
GTAAGTAGTGAATCATCAGT+AGG - chr8_3:65866392-65866411 None:intergenic 35.0%
TGATGATTCACTACTTACTC+AGG + chr8_3:65866394-65866413 Msa1303090:CDS 35.0%
TTCACTACTTACTCAGGTAT+TGG + chr8_3:65866400-65866419 Msa1303090:intron 35.0%
TCACTACTTACTCAGGTATT+GGG + chr8_3:65866401-65866420 Msa1303090:intron 35.0%
GCTCAATAAACTTGTCAATG+TGG + chr8_3:65866624-65866643 Msa1303090:three_prime_UTR 35.0%
GAAACTCATAAAACATGGTG+TGG + chr8_3:65866657-65866676 Msa1303090:three_prime_UTR 35.0%
TAAAACATGGTGTGGAGAAA+AGG + chr8_3:65866665-65866684 Msa1303090:three_prime_UTR 35.0%
GGACACTTAAAGAATTCATC+TGG - chr8_3:65866737-65866756 None:intergenic 35.0%
! TGCTCTTTTCAACTAGTTGA+TGG + chr8_3:65866810-65866829 Msa1303090:three_prime_UTR 35.0%
TTGCAAGTTATAAAGCTGGT+TGG - chr8_3:65866914-65866933 None:intergenic 35.0%
GATCAATGCGGTTAACTTAT+AGG + chr8_3:65866959-65866978 Msa1303090:three_prime_UTR 35.0%
TCAATGCGGTTAACTTATAG+GGG + chr8_3:65866961-65866980 Msa1303090:three_prime_UTR 35.0%
TTAGTTGTGTCTAAGGGTTA+AGG + chr8_3:65867074-65867093 Msa1303090:three_prime_UTR 35.0%
!!! TAAGAGATTTTTTGGTGCGG+TGG - chr8_3:65864257-65864276 None:intergenic 40.0%
TATGTGTGAGGAGAAGAAGA+TGG - chr8_3:65864286-65864305 None:intergenic 40.0%
TGGGTAATGTGTTATGTGTG+AGG - chr8_3:65864298-65864317 None:intergenic 40.0%
GTTACATAGTCATCAAGTCC+AGG + chr8_3:65864436-65864455 Msa1303090:CDS 40.0%
GAAAAGCAGAAAACCTTGCA+AGG - chr8_3:65864462-65864481 None:intergenic 40.0%
TCCCTATAATCCTTGACAAC+AGG + chr8_3:65865128-65865147 Msa1303090:intron 40.0%
CCCTGTTGTCAAGGATTATA+GGG - chr8_3:65865132-65865151 None:intergenic 40.0%
CCCTATAATCCTTGACAACA+GGG + chr8_3:65865129-65865148 Msa1303090:intron 40.0%
TCCCTGTTGTCAAGGATTAT+AGG - chr8_3:65865133-65865152 None:intergenic 40.0%
ACATCACAATTGTGGGATGA+AGG - chr8_3:65865369-65865388 None:intergenic 40.0%
ATCATCTTTGCTCTCTTTGC+AGG + chr8_3:65865444-65865463 Msa1303090:intron 40.0%
CTTGTGACAACTTTAGTAGG+TGG + chr8_3:65865540-65865559 Msa1303090:intron 40.0%
TGCTGTAAACCTATGAAGCA+CGG + chr8_3:65865938-65865957 Msa1303090:intron 40.0%
!! TATAAGTGTCGGTGTTGTGT+CGG + chr8_3:65866028-65866047 Msa1303090:intron 40.0%
!! GTGAGTTTGTTGACTGACAA+TGG + chr8_3:65866341-65866360 Msa1303090:CDS 40.0%
AGGAAGCTTCAGATCATCTT+TGG - chr8_3:65866372-65866391 None:intergenic 40.0%
GTTTCTGTTATGTCTGCGAT+GGG + chr8_3:65866537-65866556 Msa1303090:CDS 40.0%
! ACTAGTTGAAAAGAGCACAG+AGG - chr8_3:65866807-65866826 None:intergenic 40.0%
! GGTCGATTAGTTGTGTCTAA+GGG + chr8_3:65867068-65867087 Msa1303090:three_prime_UTR 40.0%
ATCCAAAACCTACCCTCAAC+AGG + chr8_3:65864392-65864411 Msa1303090:CDS 45.0%
GAATAGTACCAGCCTGTTGA+GGG - chr8_3:65864407-65864426 None:intergenic 45.0%
GCTGGTACTATTCGCAAGAA+TGG + chr8_3:65864414-65864433 Msa1303090:CDS 45.0%
TTCAAAACCTAGGGCCTGTT+TGG - chr8_3:65865011-65865030 None:intergenic 45.0%
CGACATCGTTAGGATGAGTT+AGG + chr8_3:65865057-65865076 Msa1303090:intron 45.0%
ATCCTTGACAACAGGGACAT+TGG + chr8_3:65865136-65865155 Msa1303090:intron 45.0%
TTCCAATGTCCCTGTTGTCA+AGG - chr8_3:65865141-65865160 None:intergenic 45.0%
TGTCTGTATCCGTGCTTCAT+AGG - chr8_3:65865950-65865969 None:intergenic 45.0%
TGCTGATGGAAAAGATCTCG+TGG + chr8_3:65866515-65866534 Msa1303090:CDS 45.0%
GGTTTCTGTTATGTCTGCGA+TGG + chr8_3:65866536-65866555 Msa1303090:CDS 45.0%
ATATGTGCCCTGAAGGACAT+TGG + chr8_3:65866570-65866589 Msa1303090:CDS 45.0%
CTTAGGACCAATGTCCTTCA+GGG - chr8_3:65866580-65866599 None:intergenic 45.0%
ACTTAGGACCAATGTCCTTC+AGG - chr8_3:65866581-65866600 None:intergenic 45.0%
! AAGAGCACAGAGGAGAGATA+GGG - chr8_3:65866797-65866816 None:intergenic 45.0%
! AAAGAGCACAGAGGAGAGAT+AGG - chr8_3:65866798-65866817 None:intergenic 45.0%
CGGTTAACTTATAGGGGATG+CGG + chr8_3:65866967-65866986 Msa1303090:three_prime_UTR 45.0%
! CGGTCGATTAGTTGTGTCTA+AGG + chr8_3:65867067-65867086 Msa1303090:three_prime_UTR 45.0%
!!! AGGGTAGGTTTTGGATGCTC+CGG - chr8_3:65864388-65864407 None:intergenic 50.0%
! AGCCTGTTGAGGGTAGGTTT+TGG - chr8_3:65864397-65864416 None:intergenic 50.0%
AAAACCTACCCTCAACAGGC+TGG + chr8_3:65864396-65864415 Msa1303090:CDS 50.0%
CGAATAGTACCAGCCTGTTG+AGG - chr8_3:65864408-65864427 None:intergenic 50.0%
AGTCAATCCAAACAGGCCCT+AGG + chr8_3:65865001-65865020 Msa1303090:intron 50.0%
GACACGTAGTCGACATCGTT+AGG + chr8_3:65865047-65865066 Msa1303090:intron 50.0%
TGAAGCACGGATACAGACAC+CGG + chr8_3:65865951-65865970 Msa1303090:intron 50.0%
AGAGCAGATATGTGCCCTGA+AGG + chr8_3:65866563-65866582 Msa1303090:CDS 50.0%
GTGTGTGATGTAGTGTGCAC+TGG + chr8_3:65866855-65866874 Msa1303090:three_prime_UTR 50.0%
CTTATAGGGGATGCGGATCT+AGG + chr8_3:65866974-65866993 Msa1303090:three_prime_UTR 50.0%
!!! GGTTTTGGATGCTCCGGCAT+CGG - chr8_3:65864382-65864401 None:intergenic 55.0%
AGTACCAGCCTGTTGAGGGT+AGG - chr8_3:65864403-65864422 None:intergenic 55.0%
CATCAAGTCCAGGCCTTGCA+AGG + chr8_3:65864446-65864465 Msa1303090:CDS 55.0%
GCAGAAAACCTTGCAAGGCC+TGG - chr8_3:65864457-65864476 None:intergenic 55.0%
ACGGATACAGACACCGGACA+CGG + chr8_3:65865957-65865976 Msa1303090:intron 55.0%
GTTATGCCAGTGTCCGTGTC+CGG - chr8_3:65865973-65865992 None:intergenic 55.0%
GATGCTTTCTCTGCGCGCGT+GGG - chr8_3:65864317-65864336 None:intergenic 60.0%
TGATGCTTTCTCTGCGCGCG+TGG - chr8_3:65864318-65864337 None:intergenic 60.0%
TTTGAACCCGCTGCCGATGC+CGG + chr8_3:65864366-65864385 Msa1303090:CDS 60.0%
CAGACACCGGACACGGACAC+TGG + chr8_3:65865964-65865983 Msa1303090:intron 65.0%
!! GATGCTCCGGCATCGGCAGC+GGG - chr8_3:65864375-65864394 None:intergenic 70.0%
!! GGATGCTCCGGCATCGGCAG+CGG - chr8_3:65864376-65864395 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 65864218 65867195 65864218 ID=Msa1303090;Name=Msa1303090
chr8_3 mRNA 65864218 65867195 65864218 ID=Msa1303090-mRNA-1;Parent=Msa1303090;Name=Msa1303090-mRNA-1;_AED=0.02;_eAED=0.02;_QI=127|1|1|1|1|1|5|593|159
chr8_3 exon 65864218 65864467 65864218 ID=Msa1303090-mRNA-1:exon:20012;Parent=Msa1303090-mRNA-1
chr8_3 exon 65865264 65865386 65865264 ID=Msa1303090-mRNA-1:exon:20013;Parent=Msa1303090-mRNA-1
chr8_3 exon 65865466 65865522 65865466 ID=Msa1303090-mRNA-1:exon:20014;Parent=Msa1303090-mRNA-1
chr8_3 exon 65866341 65866415 65866341 ID=Msa1303090-mRNA-1:exon:20015;Parent=Msa1303090-mRNA-1
chr8_3 exon 65866501 65867195 65866501 ID=Msa1303090-mRNA-1:exon:20016;Parent=Msa1303090-mRNA-1
chr8_3 five_prime_UTR 65864218 65864344 65864218 ID=Msa1303090-mRNA-1:five_prime_utr;Parent=Msa1303090-mRNA-1
chr8_3 CDS 65864345 65864467 65864345 ID=Msa1303090-mRNA-1:cds;Parent=Msa1303090-mRNA-1
chr8_3 CDS 65865264 65865386 65865264 ID=Msa1303090-mRNA-1:cds;Parent=Msa1303090-mRNA-1
chr8_3 CDS 65865466 65865522 65865466 ID=Msa1303090-mRNA-1:cds;Parent=Msa1303090-mRNA-1
chr8_3 CDS 65866341 65866415 65866341 ID=Msa1303090-mRNA-1:cds;Parent=Msa1303090-mRNA-1
chr8_3 CDS 65866501 65866602 65866501 ID=Msa1303090-mRNA-1:cds;Parent=Msa1303090-mRNA-1
chr8_3 three_prime_UTR 65866603 65867195 65866603 ID=Msa1303090-mRNA-1:three_prime_utr;Parent=Msa1303090-mRNA-1
Gene Sequence

>Msa1303090

ATGTCTGACGAAGAACATCACTTTGAACCCGCTGCCGATGCCGGAGCATCCAAAACCTACCCTCAACAGGCTGGTACTATTCGCAAGAATGGTTACATAGTCATCAAGTCCAGGCCTTGCAAGGTTGTTGAGGTTTCTACTTCAAAAACAGGAAAACATGGACATGCAAAGTGTCACTTTGTTGCTATTGATATTTTCAATGGGAAAAAACTTGAAGATATTGTTCCTTCATCCCACAATTGTGATGTTCCTCATGTGAATCGTACTGATTATCAGTTGATTGATATTTCTGAAGATGGATTTGTGAGTTTGTTGACTGACAATGGAAGTACCAAAGATGATCTGAAGCTTCCTACTGATGATTCACTACTTACTCAGATTAAAGATGGATTTGCTGATGGAAAAGATCTCGTGGTTTCTGTTATGTCTGCGATGGGTGAAGAGCAGATATGTGCCCTGAAGGACATTGGTCCTAAGTAG
Protein sequence

>Msa1303090

MSDEEHHFEPAADAGASKTYPQQAGTIRKNGYIVIKSRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDDSLLTQIKDGFADGKDLVVSVMSAMGEEQICALKDIGPK*