AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1370470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1370470 MtrunA17_Chr4g0074021 100.000 159 0 0 1 159 1 159 5.08e-117 327
Msa1370470 MtrunA17_Chr4g0061171 93.711 159 10 0 1 159 1 159 2.71e-109 308
Msa1370470 MtrunA17_Chr3g0091481 92.453 159 12 0 1 159 1 159 1.42e-108 306
Msa1370470 MtrunA17_Chr5g0418211 89.937 159 16 0 1 159 1 159 1.75e-106 300
Msa1370470 MtrunA17_Chr4g0062241 68.627 102 22 1 1 102 1 92 3.17e-45 143
Msa1370470 MtrunA17_Chr4g0074011 47.656 128 19 2 18 145 42 121 1.06e-26 98.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1370470 AT1G13950.1 91.824 159 12 1 1 159 1 158 4.28e-105 297
Msa1370470 AT1G69410.1 86.792 159 20 1 1 159 1 158 9.26e-102 289
Msa1370470 AT1G26630.1 81.529 157 28 1 1 157 1 156 1.42e-93 268
Msa1370470 AT1G26630.2 84.252 127 19 1 1 127 1 126 6.93e-76 223

Find 64 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATATCCAATAAGTTATTT+TGG 0.141811 tig0019129:+73175 Msa1370470:three_prime_UTR
TTCATCCCAAAATAACTTAT+TGG 0.178416 tig0019129:-73181 None:intergenic
GATCAATGCGGTTAACTTAT+AGG 0.258167 tig0019129:+72962 Msa1370470:three_prime_UTR
ATTATTGAACTCTCTGGTTT+TGG 0.270929 tig0019129:+72714 Msa1370470:three_prime_UTR
ATCAATGCGGTTAACTTATA+GGG 0.274474 tig0019129:+72963 Msa1370470:three_prime_UTR
CGGTCGATTAGTTGTGTCTA+AGG 0.307333 tig0019129:+73070 Msa1370470:three_prime_UTR
AAAGAGCACAGAGGAGAGAT+AGG 0.326363 tig0019129:-72798 None:intergenic
AGCCTGTTGAGGGTAGGTTT+TGG 0.330390 tig0019129:-70396 None:intergenic
AGGAAGCTTCAGATCATCTT+TGG 0.330486 tig0019129:-72372 None:intergenic
ACTTAGGACCAATGTCCTTC+AGG 0.343359 tig0019129:-72581 None:intergenic
TTGATTGATATTTCTGAAGA+TGG 0.371340 tig0019129:+71499 Msa1370470:CDS
CTTATAGGGGATGCGGATCT+AGG 0.397089 tig0019129:+72977 Msa1370470:three_prime_UTR
TCTTTAATTATTGAACTCTC+TGG 0.403510 tig0019129:+72708 Msa1370470:three_prime_UTR
CTGAGTAGTTGTGTATATAT+TGG 0.412034 tig0019129:+73046 Msa1370470:three_prime_UTR
TGAGGATCGGATGAATGATT+AGG 0.418550 tig0019129:+70208 None:intergenic
GCAGAAAACCTTGCAAGGCC+TGG 0.423629 tig0019129:-70456 None:intergenic
TTAAAGAGACAAGATAAATA+TGG 0.437632 tig0019129:-72692 None:intergenic
ATCCAAAACCTACCCTCAAC+AGG 0.440341 tig0019129:+70394 Msa1370470:CDS
ATACATTCATAGATTAAAGA+TGG 0.442913 tig0019129:+72492 Msa1370470:intron
TAAAACATGGTGTGGAGAAA+AGG 0.446845 tig0019129:+72668 Msa1370470:three_prime_UTR
GGATGCTCCGGCATCGGCAG+CGG 0.446886 tig0019129:-70375 None:intergenic
GATGCTCCGGCATCGGCAGC+GGG 0.447940 tig0019129:-70374 None:intergenic
TTTGAACCCGCTGCCGATGC+CGG 0.456716 tig0019129:+70368 Msa1370470:CDS
TTAGTTGTGTCTAAGGGTTA+AGG 0.475769 tig0019129:+73077 Msa1370470:three_prime_UTR
CTTAGGACCAATGTCCTTCA+GGG 0.484561 tig0019129:-72580 None:intergenic
ATTAAAGATGGATTTGCTGA+TGG 0.488295 tig0019129:+72504 Msa1370470:CDS
GGACACTTAAAGAATTCATC+TGG 0.489024 tig0019129:-72737 None:intergenic
GAATAGTACCAGCCTGTTGA+GGG 0.502881 tig0019129:-70406 None:intergenic
GGTTTCTGTTATGTCTGCGA+TGG 0.503255 tig0019129:+72539 Msa1370470:CDS
ACATCACAATTGTGGGATGA+AGG 0.511953 tig0019129:-71369 None:intergenic
CTAACTTACATCACAATTGT+GGG 0.513530 tig0019129:-71376 None:intergenic
TCTAACTTACATCACAATTG+TGG 0.519354 tig0019129:-71377 None:intergenic
CATCAAGTCCAGGCCTTGCA+AGG 0.525229 tig0019129:+70448 Msa1370470:CDS
GCTGGTACTATTCGCAAGAA+TGG 0.527051 tig0019129:+70416 Msa1370470:CDS
CGAATAGTACCAGCCTGTTG+AGG 0.540601 tig0019129:-70407 None:intergenic
TTGATTGCAAGTTATAAAGC+TGG 0.545199 tig0019129:-72918 None:intergenic
CGGTTAACTTATAGGGGATG+CGG 0.553112 tig0019129:+72970 Msa1370470:three_prime_UTR
GTTTCTGTTATGTCTGCGAT+GGG 0.555762 tig0019129:+72540 Msa1370470:CDS
GTGTGTGATGTAGTGTGCAC+TGG 0.568452 tig0019129:+72858 Msa1370470:three_prime_UTR
TTGCAAGTTATAAAGCTGGT+TGG 0.569207 tig0019129:-72914 None:intergenic
AAGAGCACAGAGGAGAGATA+GGG 0.569296 tig0019129:-72797 None:intergenic
GGTCGATTAGTTGTGTCTAA+GGG 0.576561 tig0019129:+73071 Msa1370470:three_prime_UTR
AAAACCTACCCTCAACAGGC+TGG 0.584266 tig0019129:+70398 Msa1370470:CDS
GATGCTTTCTCTGCGCGCGT+GGG 0.584738 tig0019129:-70316 None:intergenic
GAAACTCATAAAACATGGTG+TGG 0.589277 tig0019129:+72660 Msa1370470:three_prime_UTR
AGAGCAGATATGTGCCCTGA+AGG 0.589389 tig0019129:+72566 Msa1370470:CDS
TGCTGATGGAAAAGATCTCG+TGG 0.593109 tig0019129:+72518 Msa1370470:CDS
GAAAAGCAGAAAACCTTGCA+AGG 0.593565 tig0019129:-70461 None:intergenic
ATATGTGCCCTGAAGGACAT+TGG 0.593679 tig0019129:+72573 Msa1370470:CDS
GTTACATAGTCATCAAGTCC+AGG 0.598114 tig0019129:+70438 Msa1370470:CDS
TGATGCTTTCTCTGCGCGCG+TGG 0.598798 tig0019129:-70317 None:intergenic
TGATGATTCACTACTTACTC+AGG 0.602143 tig0019129:+72397 Msa1370470:CDS
TCAATGCGGTTAACTTATAG+GGG 0.604359 tig0019129:+72964 Msa1370470:three_prime_UTR
GTAAGTAGTGAATCATCAGT+AGG 0.605999 tig0019129:-72392 None:intergenic
AGTACCAGCCTGTTGAGGGT+AGG 0.611010 tig0019129:-70402 None:intergenic
GTGAGTTTGTTGACTGACAA+TGG 0.614472 tig0019129:+72344 Msa1370470:CDS
TATGTGTGAGGAGAAGAAGA+TGG 0.621382 tig0019129:-70285 None:intergenic
GTAGTTGTGTATATATTGGA+CGG 0.637381 tig0019129:+73050 Msa1370470:three_prime_UTR
GCTCAATAAACTTGTCAATG+TGG 0.641522 tig0019129:+72627 Msa1370470:three_prime_UTR
AGCTTGAAACTCATAAAACA+TGG 0.668434 tig0019129:+72655 Msa1370470:three_prime_UTR
TTTATAACTTGCAATCAACA+CGG 0.675149 tig0019129:+72923 Msa1370470:three_prime_UTR
TGGGTAATGTGTTATGTGTG+AGG 0.693706 tig0019129:-70297 None:intergenic
TAATCAGTACGATTCACATG+AGG 0.718954 tig0019129:-71472 None:intergenic
ATTAGTTGAAAAGAGCACAG+AGG 0.742860 tig0019129:-72807 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGATTTGATTGTTTTAAA+CGG + tig0019129:70696-70715 Msa1370470:intron 15.0%
!!! TTTGAAACAATTTTTAAAGA+TGG + tig0019129:71028-71047 Msa1370470:intron 15.0%
!!! TTTTATAAACTTGATATAAC+TGG + tig0019129:71090-71109 Msa1370470:intron 15.0%
!! AATATATTGAGAAACAAATA+CGG - tig0019129:71240-71259 None:intergenic 15.0%
!!! ATTTGTTTCTCAATATATTT+TGG + tig0019129:71241-71260 Msa1370470:intron 15.0%
!! ATTAAGTTAAAACTTTCAAA+TGG + tig0019129:71569-71588 Msa1370470:intron 15.0%
!! ACAAAAATATAAAGTTACAA+GGG - tig0019129:72761-72780 None:intergenic 15.0%
!! AACAAAAATATAAAGTTACA+AGG - tig0019129:72762-72781 None:intergenic 15.0%
!!! ATATATCCAATAAGTTATTT+TGG + tig0019129:73175-73194 Msa1370470:three_prime_UTR 15.0%
!!! TTTAGAATAAAAAGAGAGTT+AGG + tig0019129:70231-70250 Msa1370470:five_prime_UTR 20.0%
!! TTAGAATAAAAAGAGAGTTA+GGG + tig0019129:70232-70251 Msa1370470:five_prime_UTR 20.0%
!! AAAAAATACAAATCTAGAAG+GGG - tig0019129:70492-70511 None:intergenic 20.0%
!! CAAAAAATACAAATCTAGAA+GGG - tig0019129:70493-70512 None:intergenic 20.0%
!! TCAAAAAATACAAATCTAGA+AGG - tig0019129:70494-70513 None:intergenic 20.0%
!! TGTTTGAATCGAATAAAAAT+TGG + tig0019129:70652-70671 Msa1370470:intron 20.0%
!! ATAAAAATTGGAACATGATT+TGG + tig0019129:70664-70683 Msa1370470:intron 20.0%
!! AAAAATTGTTTCAAAACCTA+GGG - tig0019129:71023-71042 None:intergenic 20.0%
!! TAAAAATTGTTTCAAAACCT+AGG - tig0019129:71024-71043 None:intergenic 20.0%
!!! TTGCTATTGATATTTTCAAT+GGG + tig0019129:71325-71344 Msa1370470:CDS 20.0%
!! GGATATATAAAATGAGAATT+AGG + tig0019129:71590-71609 Msa1370470:intron 20.0%
!! TGTTATCTTAATGTCTATTA+AGG + tig0019129:71640-71659 Msa1370470:intron 20.0%
!! GTTATCTTAATGTCTATTAA+GGG + tig0019129:71641-71660 Msa1370470:intron 20.0%
!!! ATAAAACATACTCAGTTTTT+AGG - tig0019129:71685-71704 None:intergenic 20.0%
!! AAATATTATAGTCATTCCTT+TGG - tig0019129:71792-71811 None:intergenic 20.0%
!!! TTTACATTATGTTTTATGTC+AGG + tig0019129:71823-71842 Msa1370470:intron 20.0%
!! TTTAGTGTAATTATAAGTGT+CGG + tig0019129:72020-72039 Msa1370470:intron 20.0%
!! AAGTAAAAATAAGATGACAT+AGG + tig0019129:72125-72144 Msa1370470:intron 20.0%
!! AGTAAAAATAAGATGACATA+GGG + tig0019129:72126-72145 Msa1370470:intron 20.0%
!! AAAGAGACAAAATATATATG+TGG - tig0019129:72457-72476 None:intergenic 20.0%
!!! GTCTCTTTAATTTGATTTAT+TGG + tig0019129:72469-72488 Msa1370470:intron 20.0%
!! ATACATTCATAGATTAAAGA+TGG + tig0019129:72492-72511 Msa1370470:intron 20.0%
!! TTAAAGAGACAAGATAAATA+TGG - tig0019129:72695-72714 None:intergenic 20.0%
!! TGATGGAGAAAAATAAATTT+AGG + tig0019129:72830-72849 Msa1370470:three_prime_UTR 20.0%
! AATTCCTTATTCGATTAATC+TGG + tig0019129:70513-70532 Msa1370470:intron 25.0%
! TTAACATGCTTCTGTTTATA+TGG + tig0019129:70599-70618 Msa1370470:intron 25.0%
!!! TGTTTATATGGTGTTGATTT+TGG + tig0019129:70611-70630 Msa1370470:intron 25.0%
! TTTACATCTAGATCTAACAT+TGG + tig0019129:70741-70760 Msa1370470:intron 25.0%
!! AAGTTAATCTCTTTTCAACA+TGG - tig0019129:70800-70819 None:intergenic 25.0%
!!! AATTGTGCTAACTTGTTTAT+TGG + tig0019129:71211-71230 Msa1370470:intron 25.0%
!!! ATTGTGCTAACTTGTTTATT+GGG + tig0019129:71212-71231 Msa1370470:intron 25.0%
!!! GTTTCTCAATATATTTTGGT+AGG + tig0019129:71245-71264 Msa1370470:intron 25.0%
!!! GTTGCTATTGATATTTTCAA+TGG + tig0019129:71324-71343 Msa1370470:CDS 25.0%
!! TTGATTGATATTTCTGAAGA+TGG + tig0019129:71499-71518 Msa1370470:CDS 25.0%
! GTTATATAGATGAGTTACAT+GGG + tig0019129:71612-71631 Msa1370470:intron 25.0%
! TTATATAGATGAGTTACATG+GGG + tig0019129:71613-71632 Msa1370470:intron 25.0%
!! TTCACAGTAAATTTTACCAA+AGG + tig0019129:71773-71792 Msa1370470:intron 25.0%
! TTCTTCATGTTAGGAAAATA+TGG - tig0019129:71878-71897 None:intergenic 25.0%
!! TATTTGACTTTCTTCATGTT+AGG - tig0019129:71887-71906 None:intergenic 25.0%
!! ACTTGTTTGTATTCACATTT+TGG - tig0019129:72241-72260 None:intergenic 25.0%
! CATACGAAAAATTACTACTT+AGG - tig0019129:72600-72619 None:intergenic 25.0%
! TCTTTAATTATTGAACTCTC+TGG + tig0019129:72708-72727 Msa1370470:three_prime_UTR 25.0%
!! TTTATAACTTGCAATCAACA+CGG + tig0019129:72923-72942 Msa1370470:three_prime_UTR 25.0%
!!! AGTTAAGAGATTTTTTGGTG+CGG - tig0019129:70262-70281 None:intergenic 30.0%
!!! GGATGAGTTAAGAGATTTTT+TGG - tig0019129:70267-70286 None:intergenic 30.0%
GATTCCAGATTAATCGAATA+AGG - tig0019129:70520-70539 None:intergenic 30.0%
!!! ATTTTGGTTGATTGGTAACT+GGG + tig0019129:70627-70646 Msa1370470:intron 30.0%
TCAAATAAGTCAATCCAAAC+AGG + tig0019129:70997-71016 Msa1370470:intron 30.0%
!!! CATTGGAATTTTAGTTTTGC+TGG + tig0019129:71156-71175 Msa1370470:intron 30.0%
!!! TATATTTTGGTAGGTTGTTG+AGG + tig0019129:71254-71273 Msa1370470:intron 30.0%
ACTTCAAAAACAGGAAAACA+TGG + tig0019129:71282-71301 Msa1370470:CDS 30.0%
CTAACTTACATCACAATTGT+GGG - tig0019129:71379-71398 None:intergenic 30.0%
TCTAACTTACATCACAATTG+TGG - tig0019129:71380-71399 None:intergenic 30.0%
TTACTTGTGACAACTTTAGT+AGG + tig0019129:71540-71559 Msa1370470:intron 30.0%
GGTTATATAGATGAGTTACA+TGG + tig0019129:71611-71630 Msa1370470:intron 30.0%
TATATAGATGAGTTACATGG+GGG + tig0019129:71614-71633 Msa1370470:intron 30.0%
TTAATGTCTATTAAGGGATG+AGG + tig0019129:71647-71666 Msa1370470:intron 30.0%
TAATGTCTATTAAGGGATGA+GGG + tig0019129:71648-71667 Msa1370470:intron 30.0%
TTCTTCATGTTAGGAAAACA+AGG - tig0019129:71743-71762 None:intergenic 30.0%
TAGTTGACATTCTTCATGTT+AGG - tig0019129:71752-71771 None:intergenic 30.0%
CCTAAACAAAACATTCATGT+AGG - tig0019129:72325-72344 None:intergenic 30.0%
!!! CCTACATGAATGTTTTGTTT+AGG + tig0019129:72322-72341 Msa1370470:intron 30.0%
!! ATTAAAGATGGATTTGCTGA+TGG + tig0019129:72504-72523 Msa1370470:CDS 30.0%
AGCTTGAAACTCATAAAACA+TGG + tig0019129:72655-72674 Msa1370470:three_prime_UTR 30.0%
!! ATTATTGAACTCTCTGGTTT+TGG + tig0019129:72714-72733 Msa1370470:three_prime_UTR 30.0%
! TGCTCTTTTCAACTAATTGA+TGG + tig0019129:72813-72832 Msa1370470:three_prime_UTR 30.0%
TTGATTGCAAGTTATAAAGC+TGG - tig0019129:72921-72940 None:intergenic 30.0%
!!! ACACGGTTAACTTTTTTCAA+AGG + tig0019129:72940-72959 Msa1370470:three_prime_UTR 30.0%
!!! CTTTTTTCAAAGGATCAATG+CGG + tig0019129:72950-72969 Msa1370470:three_prime_UTR 30.0%
ATCAATGCGGTTAACTTATA+GGG + tig0019129:72963-72982 Msa1370470:three_prime_UTR 30.0%
CTGAGTAGTTGTGTATATAT+TGG + tig0019129:73046-73065 Msa1370470:three_prime_UTR 30.0%
GTAGTTGTGTATATATTGGA+CGG + tig0019129:73050-73069 Msa1370470:three_prime_UTR 30.0%
TACAAATCTAGAAGGGGAAA+GGG - tig0019129:70486-70505 None:intergenic 35.0%
ATACAAATCTAGAAGGGGAA+AGG - tig0019129:70487-70506 None:intergenic 35.0%
!!! TGGTGTTGATTTTGGTTGAT+TGG + tig0019129:70619-70638 Msa1370470:intron 35.0%
!!! GATTTTGGTTGATTGGTAAC+TGG + tig0019129:70626-70645 Msa1370470:intron 35.0%
TAAACGGAGACATAGACATA+TGG + tig0019129:70712-70731 Msa1370470:intron 35.0%
GAGGTTTCTACTTCAAAAAC+AGG + tig0019129:71273-71292 Msa1370470:CDS 35.0%
TAATCAGTACGATTCACATG+AGG - tig0019129:71475-71494 None:intergenic 35.0%
TTGTGACAACTTTAGTAGGT+GGG + tig0019129:71544-71563 Msa1370470:intron 35.0%
!!! CATACTCAGTTTTTAGGACA+TGG - tig0019129:71679-71698 None:intergenic 35.0%
GAGTTTACAACTATGAAGCA+TGG - tig0019129:72070-72089 None:intergenic 35.0%
ACAAGTGCACAACAAGAAAA+AGG + tig0019129:72255-72274 Msa1370470:intron 35.0%
CAAGTGCACAACAAGAAAAA+GGG + tig0019129:72256-72275 Msa1370470:intron 35.0%
TTATGCTATTGTCATTGCAG+TGG + tig0019129:72287-72306 Msa1370470:intron 35.0%
GTAAGTAGTGAATCATCAGT+AGG - tig0019129:72395-72414 None:intergenic 35.0%
TGATGATTCACTACTTACTC+AGG + tig0019129:72397-72416 Msa1370470:CDS 35.0%
TTCACTACTTACTCAGGTAT+TGG + tig0019129:72403-72422 Msa1370470:intron 35.0%
TCACTACTTACTCAGGTATT+GGG + tig0019129:72404-72423 Msa1370470:intron 35.0%
GCTCAATAAACTTGTCAATG+TGG + tig0019129:72627-72646 Msa1370470:three_prime_UTR 35.0%
GAAACTCATAAAACATGGTG+TGG + tig0019129:72660-72679 Msa1370470:three_prime_UTR 35.0%
TAAAACATGGTGTGGAGAAA+AGG + tig0019129:72668-72687 Msa1370470:three_prime_UTR 35.0%
GGACACTTAAAGAATTCATC+TGG - tig0019129:72740-72759 None:intergenic 35.0%
! ATTAGTTGAAAAGAGCACAG+AGG - tig0019129:72810-72829 None:intergenic 35.0%
TTGCAAGTTATAAAGCTGGT+TGG - tig0019129:72917-72936 None:intergenic 35.0%
GATCAATGCGGTTAACTTAT+AGG + tig0019129:72962-72981 Msa1370470:three_prime_UTR 35.0%
TCAATGCGGTTAACTTATAG+GGG + tig0019129:72964-72983 Msa1370470:three_prime_UTR 35.0%
TTAGTTGTGTCTAAGGGTTA+AGG + tig0019129:73077-73096 Msa1370470:three_prime_UTR 35.0%
!!! TAAGAGATTTTTTGGTGCGG+TGG - tig0019129:70259-70278 None:intergenic 40.0%
TATGTGTGAGGAGAAGAAGA+TGG - tig0019129:70288-70307 None:intergenic 40.0%
TGGGTAATGTGTTATGTGTG+AGG - tig0019129:70300-70319 None:intergenic 40.0%
GTTACATAGTCATCAAGTCC+AGG + tig0019129:70438-70457 Msa1370470:CDS 40.0%
GAAAAGCAGAAAACCTTGCA+AGG - tig0019129:70464-70483 None:intergenic 40.0%
TCCCTATAATCCTTGACAAC+AGG + tig0019129:71131-71150 Msa1370470:intron 40.0%
CCCTGTTGTCAAGGATTATA+GGG - tig0019129:71135-71154 None:intergenic 40.0%
CCCTATAATCCTTGACAACA+GGG + tig0019129:71132-71151 Msa1370470:intron 40.0%
TCCCTGTTGTCAAGGATTAT+AGG - tig0019129:71136-71155 None:intergenic 40.0%
ACATCACAATTGTGGGATGA+AGG - tig0019129:71372-71391 None:intergenic 40.0%
ATCATCTTTGCTCTCTTTGC+AGG + tig0019129:71447-71466 Msa1370470:intron 40.0%
CTTGTGACAACTTTAGTAGG+TGG + tig0019129:71543-71562 Msa1370470:intron 40.0%
TGCTGTAAACCTATGAAGCA+CGG + tig0019129:71941-71960 Msa1370470:intron 40.0%
!! TATAAGTGTCGGTGTTGTGT+CGG + tig0019129:72031-72050 Msa1370470:intron 40.0%
!! GTGAGTTTGTTGACTGACAA+TGG + tig0019129:72344-72363 Msa1370470:CDS 40.0%
AGGAAGCTTCAGATCATCTT+TGG - tig0019129:72375-72394 None:intergenic 40.0%
GTTTCTGTTATGTCTGCGAT+GGG + tig0019129:72540-72559 Msa1370470:CDS 40.0%
! GGTCGATTAGTTGTGTCTAA+GGG + tig0019129:73071-73090 Msa1370470:three_prime_UTR 40.0%
ATCCAAAACCTACCCTCAAC+AGG + tig0019129:70394-70413 Msa1370470:CDS 45.0%
GAATAGTACCAGCCTGTTGA+GGG - tig0019129:70409-70428 None:intergenic 45.0%
GCTGGTACTATTCGCAAGAA+TGG + tig0019129:70416-70435 Msa1370470:CDS 45.0%
TTCAAAACCTAGGGCCTGTT+TGG - tig0019129:71014-71033 None:intergenic 45.0%
CGACATCGTTAGGATGAGTT+AGG + tig0019129:71060-71079 Msa1370470:intron 45.0%
ATCCTTGACAACAGGGACAT+TGG + tig0019129:71139-71158 Msa1370470:intron 45.0%
TTCCAATGTCCCTGTTGTCA+AGG - tig0019129:71144-71163 None:intergenic 45.0%
TGTCTGTATCCGTGCTTCAT+AGG - tig0019129:71953-71972 None:intergenic 45.0%
TGCTGATGGAAAAGATCTCG+TGG + tig0019129:72518-72537 Msa1370470:CDS 45.0%
GGTTTCTGTTATGTCTGCGA+TGG + tig0019129:72539-72558 Msa1370470:CDS 45.0%
ATATGTGCCCTGAAGGACAT+TGG + tig0019129:72573-72592 Msa1370470:CDS 45.0%
CTTAGGACCAATGTCCTTCA+GGG - tig0019129:72583-72602 None:intergenic 45.0%
ACTTAGGACCAATGTCCTTC+AGG - tig0019129:72584-72603 None:intergenic 45.0%
! AAGAGCACAGAGGAGAGATA+GGG - tig0019129:72800-72819 None:intergenic 45.0%
! AAAGAGCACAGAGGAGAGAT+AGG - tig0019129:72801-72820 None:intergenic 45.0%
CGGTTAACTTATAGGGGATG+CGG + tig0019129:72970-72989 Msa1370470:three_prime_UTR 45.0%
! CGGTCGATTAGTTGTGTCTA+AGG + tig0019129:73070-73089 Msa1370470:three_prime_UTR 45.0%
!!! AGGGTAGGTTTTGGATGCTC+CGG - tig0019129:70390-70409 None:intergenic 50.0%
! AGCCTGTTGAGGGTAGGTTT+TGG - tig0019129:70399-70418 None:intergenic 50.0%
AAAACCTACCCTCAACAGGC+TGG + tig0019129:70398-70417 Msa1370470:CDS 50.0%
CGAATAGTACCAGCCTGTTG+AGG - tig0019129:70410-70429 None:intergenic 50.0%
AGTCAATCCAAACAGGCCCT+AGG + tig0019129:71004-71023 Msa1370470:intron 50.0%
GACACGTAGTCGACATCGTT+AGG + tig0019129:71050-71069 Msa1370470:intron 50.0%
TGAAGCACGGATACAGACAC+CGG + tig0019129:71954-71973 Msa1370470:intron 50.0%
AGAGCAGATATGTGCCCTGA+AGG + tig0019129:72566-72585 Msa1370470:CDS 50.0%
GTGTGTGATGTAGTGTGCAC+TGG + tig0019129:72858-72877 Msa1370470:three_prime_UTR 50.0%
CTTATAGGGGATGCGGATCT+AGG + tig0019129:72977-72996 Msa1370470:three_prime_UTR 50.0%
!!! GGTTTTGGATGCTCCGGCAT+CGG - tig0019129:70384-70403 None:intergenic 55.0%
AGTACCAGCCTGTTGAGGGT+AGG - tig0019129:70405-70424 None:intergenic 55.0%
CATCAAGTCCAGGCCTTGCA+AGG + tig0019129:70448-70467 Msa1370470:CDS 55.0%
GCAGAAAACCTTGCAAGGCC+TGG - tig0019129:70459-70478 None:intergenic 55.0%
ACGGATACAGACACCGGACA+CGG + tig0019129:71960-71979 Msa1370470:intron 55.0%
GTTATGCCAGTGTCCGTGTC+CGG - tig0019129:71976-71995 None:intergenic 55.0%
GATGCTTTCTCTGCGCGCGT+GGG - tig0019129:70319-70338 None:intergenic 60.0%
TGATGCTTTCTCTGCGCGCG+TGG - tig0019129:70320-70339 None:intergenic 60.0%
TTTGAACCCGCTGCCGATGC+CGG + tig0019129:70368-70387 Msa1370470:CDS 60.0%
CAGACACCGGACACGGACAC+TGG + tig0019129:71967-71986 Msa1370470:intron 65.0%
!! GATGCTCCGGCATCGGCAGC+GGG - tig0019129:70377-70396 None:intergenic 70.0%
!! GGATGCTCCGGCATCGGCAG+CGG - tig0019129:70378-70397 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0019129 gene 70220 73198 70220 ID=Msa1370470;Name=Msa1370470
tig0019129 mRNA 70220 73198 70220 ID=Msa1370470-mRNA-1;Parent=Msa1370470;Name=Msa1370470-mRNA-1;_AED=0.02;_eAED=0.02;_QI=127|1|1|1|1|1|5|593|159
tig0019129 exon 70220 70469 70220 ID=Msa1370470-mRNA-1:exon:1659;Parent=Msa1370470-mRNA-1
tig0019129 exon 71267 71389 71267 ID=Msa1370470-mRNA-1:exon:1660;Parent=Msa1370470-mRNA-1
tig0019129 exon 71469 71525 71469 ID=Msa1370470-mRNA-1:exon:1661;Parent=Msa1370470-mRNA-1
tig0019129 exon 72344 72418 72344 ID=Msa1370470-mRNA-1:exon:1662;Parent=Msa1370470-mRNA-1
tig0019129 exon 72504 73198 72504 ID=Msa1370470-mRNA-1:exon:1663;Parent=Msa1370470-mRNA-1
tig0019129 five_prime_UTR 70220 70346 70220 ID=Msa1370470-mRNA-1:five_prime_utr;Parent=Msa1370470-mRNA-1
tig0019129 CDS 70347 70469 70347 ID=Msa1370470-mRNA-1:cds;Parent=Msa1370470-mRNA-1
tig0019129 CDS 71267 71389 71267 ID=Msa1370470-mRNA-1:cds;Parent=Msa1370470-mRNA-1
tig0019129 CDS 71469 71525 71469 ID=Msa1370470-mRNA-1:cds;Parent=Msa1370470-mRNA-1
tig0019129 CDS 72344 72418 72344 ID=Msa1370470-mRNA-1:cds;Parent=Msa1370470-mRNA-1
tig0019129 CDS 72504 72605 72504 ID=Msa1370470-mRNA-1:cds;Parent=Msa1370470-mRNA-1
tig0019129 three_prime_UTR 72606 73198 72606 ID=Msa1370470-mRNA-1:three_prime_utr;Parent=Msa1370470-mRNA-1
Gene Sequence

>Msa1370470

ATGTCTGACGAAGAACATCACTTTGAACCCGCTGCCGATGCCGGAGCATCCAAAACCTACCCTCAACAGGCTGGTACTATTCGCAAGAATGGTTACATAGTCATCAAGTCCAGGCCTTGCAAGGTTGTTGAGGTTTCTACTTCAAAAACAGGAAAACATGGACATGCAAAGTGTCACTTTGTTGCTATTGATATTTTCAATGGGAAAAAACTTGAAGATATTGTTCCTTCATCCCACAATTGTGATGTTCCTCATGTGAATCGTACTGATTATCAGTTGATTGATATTTCTGAAGATGGATTTGTGAGTTTGTTGACTGACAATGGAAGTACCAAAGATGATCTGAAGCTTCCTACTGATGATTCACTACTTACTCAGATTAAAGATGGATTTGCTGATGGAAAAGATCTCGTGGTTTCTGTTATGTCTGCGATGGGTGAAGAGCAGATATGTGCCCTGAAGGACATTGGTCCTAAGTAG
Protein sequence

>Msa1370470

MSDEEHHFEPAADAGASKTYPQQAGTIRKNGYIVIKSRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDDSLLTQIKDGFADGKDLVVSVMSAMGEEQICALKDIGPK*