Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1317170 | A0A396GM00 | 91.971 | 137 | 11 | 0 | 1 | 137 | 6 | 142 | 7.58e-89 | 264 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0823400 | Msa1317170 | 0.800150 | 1.649299e-48 | -8.615850e-47 |
| Msa1317170 | Msa1458910 | 0.812205 | 4.766069e-51 | -8.615850e-47 |
| Msa0373960 | Msa1317170 | 0.802705 | 4.942753e-49 | -8.615850e-47 |
| Msa1177400 | Msa1317170 | 0.858055 | 1.071237e-62 | -8.615850e-47 |
| Msa1179520 | Msa1317170 | 0.803713 | 3.057534e-49 | -8.615850e-47 |
| Msa0591800 | Msa1317170 | 0.815897 | 7.306260e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1317170 | MtrunA17_Chr8g0347351 | 91.971 | 137 | 11 | 0 | 1 | 137 | 6 | 142 | 1.46e-92 | 264 |
| Msa1317170 | MtrunA17_Chr8g0347371 | 38.406 | 138 | 69 | 5 | 1 | 135 | 1 | 125 | 2.66e-16 | 70.1 |
| Msa1317170 | MtrunA17_Chr7g0275481 | 50.000 | 54 | 26 | 1 | 18 | 70 | 8 | 61 | 2.58e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1317170 | AT3G61660.2 | 51.724 | 58 | 24 | 1 | 8 | 65 | 2 | 55 | 6.78e-14 | 64.7 |
| Msa1317170 | AT3G61660.1 | 58.140 | 43 | 18 | 0 | 23 | 65 | 3 | 45 | 2.35e-13 | 62.8 |
Find 19 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCGTTTACTTTCTCTATA+TGG | 0.235328 | 8_4:+18290640 | None:intergenic |
| CTTCTTGGGTCACAAGCTTT+AGG | 0.271752 | 8_4:-18290617 | Msa1317170:CDS |
| AGTTCATATGAATCATATAA+AGG | 0.353897 | 8_4:+18290768 | None:intergenic |
| CTCTTGATCATCACATCAAC+AGG | 0.370624 | 8_4:-18290740 | Msa1317170:CDS |
| AGAAAGTAAACGAGTCTTCT+TGG | 0.378857 | 8_4:-18290632 | Msa1317170:CDS |
| TTTATATGATTCATATGAAC+TGG | 0.406641 | 8_4:-18290766 | Msa1317170:CDS |
| GTTCATATGAATCATATAAA+GGG | 0.444445 | 8_4:+18290769 | None:intergenic |
| TATGCATGATTCTCATGATA+TGG | 0.452143 | 8_4:-18290664 | Msa1317170:CDS |
| TCACATCAACAGGAATCTAA+TGG | 0.461388 | 8_4:-18290730 | Msa1317170:CDS |
| AGATGAACAAGAAGCATTGA+AGG | 0.464858 | 8_4:-18290847 | Msa1317170:CDS |
| GAAAGTAAACGAGTCTTCTT+GGG | 0.465397 | 8_4:-18290631 | Msa1317170:CDS |
| CAAGGAAATGAGAAGAGGTA+TGG | 0.543597 | 8_4:-18290535 | Msa1317170:CDS |
| GGTGAAGATGATGAAGAGGA+AGG | 0.547971 | 8_4:-18290586 | Msa1317170:CDS |
| AAGAACAAGGAAATGAGAAG+AGG | 0.574484 | 8_4:-18290540 | Msa1317170:CDS |
| ATGGCATTTCCATCTCTCCA+TGG | 0.584910 | 8_4:-18290711 | Msa1317170:CDS |
| GCAAGGGCAATGTGGGATTG+TGG | 0.585400 | 8_4:-18290795 | Msa1317170:CDS |
| GATGAACAAGAAGCATTGAA+GGG | 0.592575 | 8_4:-18290846 | Msa1317170:CDS |
| AAATGAGAAGAGGTATGGCT+AGG | 0.594302 | 8_4:-18290530 | Msa1317170:CDS |
| ATGAAGAAAACCAACATGAG+TGG | 0.609953 | 8_4:-18290885 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTCATATGAATCATATAAA+GGG | + | chr8_4:18290583-18290602 | None:intergenic | 20.0% |
| !! | AGTTCATATGAATCATATAA+AGG | + | chr8_4:18290584-18290603 | None:intergenic | 20.0% |
| !! | TTTATATGATTCATATGAAC+TGG | - | chr8_4:18290583-18290602 | Msa1317170:CDS | 20.0% |
| ! | AGAAAAACTAAAGAAGAACA+AGG | - | chr8_4:18290796-18290815 | Msa1317170:CDS | 25.0% |
| !! | GTAAATTTTCTTTCTTGTGA+AGG | - | chr8_4:18290848-18290867 | Msa1317170:CDS | 25.0% |
| AGAAAGAAGAAGAAAAAGCA+AGG | - | chr8_4:18290537-18290556 | Msa1317170:CDS | 30.0% | |
| GAAAGAAGAAGAAAAAGCAA+GGG | - | chr8_4:18290538-18290557 | Msa1317170:CDS | 30.0% | |
| TATGCATGATTCTCATGATA+TGG | - | chr8_4:18290685-18290704 | Msa1317170:CDS | 30.0% | |
| ACTCGTTTACTTTCTCTATA+TGG | + | chr8_4:18290712-18290731 | None:intergenic | 30.0% | |
| !! | AGATGAACAAGAAGCATTGA+AGG | - | chr8_4:18290502-18290521 | Msa1317170:CDS | 35.0% |
| !! | GATGAACAAGAAGCATTGAA+GGG | - | chr8_4:18290503-18290522 | Msa1317170:CDS | 35.0% |
| TCACATCAACAGGAATCTAA+TGG | - | chr8_4:18290619-18290638 | Msa1317170:CDS | 35.0% | |
| !! | TGATTATGCGTTTTGATCCA+TGG | + | chr8_4:18290658-18290677 | None:intergenic | 35.0% |
| AGAAAGTAAACGAGTCTTCT+TGG | - | chr8_4:18290717-18290736 | Msa1317170:CDS | 35.0% | |
| GAAAGTAAACGAGTCTTCTT+GGG | - | chr8_4:18290718-18290737 | Msa1317170:CDS | 35.0% | |
| !!! | CACAAGCTTTAGGACATTTT+TGG | - | chr8_4:18290742-18290761 | Msa1317170:CDS | 35.0% |
| ! | TTTTGGTGAAGATGATGAAG+AGG | - | chr8_4:18290759-18290778 | Msa1317170:CDS | 35.0% |
| AAGAACAAGGAAATGAGAAG+AGG | - | chr8_4:18290809-18290828 | Msa1317170:CDS | 35.0% | |
| AAGAAAAAGCAAGGGCAATG+TGG | - | chr8_4:18290546-18290565 | Msa1317170:CDS | 40.0% | |
| AGAAAAAGCAAGGGCAATGT+GGG | - | chr8_4:18290547-18290566 | Msa1317170:CDS | 40.0% | |
| CTCTTGATCATCACATCAAC+AGG | - | chr8_4:18290609-18290628 | Msa1317170:CDS | 40.0% | |
| CAAGGAAATGAGAAGAGGTA+TGG | - | chr8_4:18290814-18290833 | Msa1317170:CDS | 40.0% | |
| ! | AAATGAGAAGAGGTATGGCT+AGG | - | chr8_4:18290819-18290838 | Msa1317170:CDS | 40.0% |
| ATGGCATTTCCATCTCTCCA+TGG | - | chr8_4:18290638-18290657 | Msa1317170:CDS | 45.0% | |
| !! | CGTTTTGATCCATGGAGAGA+TGG | + | chr8_4:18290650-18290669 | None:intergenic | 45.0% |
| CTTCTTGGGTCACAAGCTTT+AGG | - | chr8_4:18290732-18290751 | Msa1317170:CDS | 45.0% | |
| GGTGAAGATGATGAAGAGGA+AGG | - | chr8_4:18290763-18290782 | Msa1317170:CDS | 45.0% | |
| GCAAGGGCAATGTGGGATTG+TGG | - | chr8_4:18290554-18290573 | Msa1317170:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 18290479 | 18290892 | 18290479 | ID=Msa1317170;Name=Msa1317170 |
| chr8_4 | mRNA | 18290479 | 18290892 | 18290479 | ID=Msa1317170-mRNA-1;Parent=Msa1317170;Name=Msa1317170-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|1|1|0|137 |
| chr8_4 | exon | 18290479 | 18290892 | 18290479 | ID=Msa1317170-mRNA-1:exon:6770;Parent=Msa1317170-mRNA-1 |
| chr8_4 | CDS | 18290479 | 18290892 | 18290479 | ID=Msa1317170-mRNA-1:cds;Parent=Msa1317170-mRNA-1 |
| Gene Sequence |
| Protein sequence |
