AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1458910


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1458910 MtrunA17_Chr3g0123851 60.656 122 8 2 1 83 1 121 3.68e-39 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 34 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATAACCTCATCTTGTTAA+TGG 0.240049 tig0039907:+16007 Msa1458910:CDS
TGTAGTGGAGGCTGAGATAG+AGG 0.383685 tig0039907:-15680 None:intergenic
ATCCGTTATTATTATTACCT+TGG 0.417680 tig0039907:-16564 None:intergenic
GGTTGATAGAAGCAAGAAAC+TGG 0.429204 tig0039907:-16062 None:intergenic
CTCCAAGGTAATAATAATAA+CGG 0.464837 tig0039907:+16562 Msa1458910:CDS
AAGAAACTGGACACAGTAGA+AGG 0.491502 tig0039907:-16049 None:intergenic
CAATGGCATTTCCAACTCAA+TGG 0.495776 tig0039907:-16601 None:intergenic
AAAGAAATTACCACCTTCCA+TGG 0.501489 tig0039907:-16539 None:intergenic
CACTGCCTCAACTTCATCCA+TGG 0.509432 tig0039907:+16522 Msa1458910:CDS
AGGGAAAAGCCTACCCTTGG+AGG 0.512819 tig0039907:-16098 None:intergenic
ATTTCTAAGGGTTGAAGAAG+TGG 0.515036 tig0039907:-16630 None:intergenic
GGAGAGGGATAGTGTAGTGG+AGG 0.517083 tig0039907:-15692 None:intergenic
CAAATTACAAGCCATTGAGT+TGG 0.518087 tig0039907:+16590 Msa1458910:CDS
GGTAACAATTGTGTAGAGAA+AGG 0.520211 tig0039907:-15713 None:intergenic
AAAGGAGAGGGATAGTGTAG+TGG 0.532448 tig0039907:-15695 None:intergenic
GGTGGTAATTTCTTTCTCCA+AGG 0.542564 tig0039907:+16547 Msa1458910:CDS
CACATTCTGCAATTCCTCCA+AGG 0.543179 tig0039907:+16084 Msa1458910:CDS
AGATAGAGGGTGCAGAGTGT+TGG 0.551815 tig0039907:-15666 None:intergenic
GAAGTTGAGGCAGTGTGACC+TGG 0.560053 tig0039907:-16514 None:intergenic
AATTGTGTAGAGAAAGGAGA+GGG 0.568206 tig0039907:-15707 None:intergenic
ACCTTCCATGGATGAAGTTG+AGG 0.576895 tig0039907:-16527 None:intergenic
GCCTCAACTTCATCCATGGA+AGG 0.583985 tig0039907:+16526 Msa1458910:CDS
ATATGAATTAAATGAAGCCA+TGG 0.598193 tig0039907:-15988 None:intergenic
GTAGTGGAGGCTGAGATAGA+GGG 0.606138 tig0039907:-15679 None:intergenic
TGATGACCATTAACAAGATG+AGG 0.607087 tig0039907:-16013 None:intergenic
TTGAGTTGGAAATGCCATTG+TGG 0.619909 tig0039907:+16604 Msa1458910:CDS
TGAAGAAGTGGCCACCACAA+TGG 0.621299 tig0039907:-16618 None:intergenic
ACATTCTGCAATTCCTCCAA+GGG 0.630675 tig0039907:+16085 Msa1458910:CDS
ACATGATAGATAGATATCCA+TGG 0.649649 tig0039907:+15971 Msa1458910:exon
AGTGTTGGCAGAAAAGTGAA+AGG 0.656008 tig0039907:-15651 None:intergenic
TCAACTTCATCCATGGAAGG+TGG 0.660618 tig0039907:+16529 Msa1458910:CDS
CTTGGAGGAATTGCAGAATG+TGG 0.672231 tig0039907:-16083 None:intergenic
AGTTGGAAATGCCATTGTGG+TGG 0.687706 tig0039907:+16607 Msa1458910:CDS
CAATTGTGTAGAGAAAGGAG+AGG 0.717317 tig0039907:-15708 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTTGAATTCACTATAAAAT+TGG - tig0039907:16292-16311 None:intergenic 20.0%
!! AGTTAACAAACTTAATTAAC+TGG - tig0039907:16367-16386 None:intergenic 20.0%
!! GTTTGAATTCACTATAAAAT+TGG - tig0039907:16292-16311 None:intergenic 20.0%
!! AGTTAACAAACTTAATTAAC+TGG - tig0039907:16367-16386 None:intergenic 20.0%
!!! GTTTTTTTCTAGTTATGTGT+TGG - tig0039907:15741-15760 None:intergenic 25.0%
!! AATGACAAACTTTTCATTCT+TGG + tig0039907:15785-15804 Msa1458910:five_prime_UTR 25.0%
!!! TTATTTTGTCACATTACAAC+TGG + tig0039907:15856-15875 Msa1458910:five_prime_UTR 25.0%
! ATATGAATTAAATGAAGCCA+TGG - tig0039907:15991-16010 None:intergenic 25.0%
! ATATAACCTCATCTTGTTAA+TGG + tig0039907:16007-16026 Msa1458910:CDS 25.0%
!!! TAGTTGGTTTTAAAAGGAAT+CGG + tig0039907:16168-16187 Msa1458910:intron 25.0%
!! ACAGTATATTACTGTGATTT+TGG + tig0039907:16226-16245 Msa1458910:intron 25.0%
! AATTCACTATAAAATTGGCT+CGG - tig0039907:16287-16306 None:intergenic 25.0%
! TATTATTTGAGGAGAAAAAC+AGG + tig0039907:16401-16420 Msa1458910:intron 25.0%
! CTCCAAGGTAATAATAATAA+CGG + tig0039907:16562-16581 Msa1458910:CDS 25.0%
! ATCCGTTATTATTATTACCT+TGG - tig0039907:16567-16586 None:intergenic 25.0%
!!! GTTTTTTTCTAGTTATGTGT+TGG - tig0039907:15741-15760 None:intergenic 25.0%
!! AATGACAAACTTTTCATTCT+TGG + tig0039907:15785-15804 Msa1458910:five_prime_UTR 25.0%
!!! TTATTTTGTCACATTACAAC+TGG + tig0039907:15856-15875 Msa1458910:five_prime_UTR 25.0%
! ATATGAATTAAATGAAGCCA+TGG - tig0039907:15991-16010 None:intergenic 25.0%
! ATATAACCTCATCTTGTTAA+TGG + tig0039907:16007-16026 Msa1458910:CDS 25.0%
!!! TAGTTGGTTTTAAAAGGAAT+CGG + tig0039907:16168-16187 Msa1458910:intron 25.0%
!! ACAGTATATTACTGTGATTT+TGG + tig0039907:16226-16245 Msa1458910:intron 25.0%
! AATTCACTATAAAATTGGCT+CGG - tig0039907:16287-16306 None:intergenic 25.0%
! TATTATTTGAGGAGAAAAAC+AGG + tig0039907:16401-16420 Msa1458910:intron 25.0%
! CTCCAAGGTAATAATAATAA+CGG + tig0039907:16562-16581 Msa1458910:CDS 25.0%
! ATCCGTTATTATTATTACCT+TGG - tig0039907:16567-16586 None:intergenic 25.0%
!!! ATCTAGTTTTGAGCAATGAA+TGG - tig0039907:15763-15782 None:intergenic 30.0%
ACATGATAGATAGATATCCA+TGG + tig0039907:15971-15990 Msa1458910:exon 30.0%
TTATGTGTATGTTGCATAGT+TGG + tig0039907:16152-16171 Msa1458910:intron 30.0%
!!! GTTGCATAGTTGGTTTTAAA+AGG + tig0039907:16162-16181 Msa1458910:intron 30.0%
ACAGTAATATACTGTGATTC+TGG - tig0039907:16221-16240 None:intergenic 30.0%
! ATCCTCATTTTGTTGAAACT+TGG - tig0039907:16320-16339 None:intergenic 30.0%
ACTTAATTAACTGGTGAGTT+TGG - tig0039907:16358-16377 None:intergenic 30.0%
!! AGTTTGTTAACTATGTGTGT+AGG + tig0039907:16375-16394 Msa1458910:intron 30.0%
! GTTTGTTAACTATGTGTGTA+GGG + tig0039907:16376-16395 Msa1458910:intron 30.0%
TGTGTAGGGTTTATTATTTG+AGG + tig0039907:16390-16409 Msa1458910:intron 30.0%
!! TTACATTTGTTGTGACATGT+AGG - tig0039907:16442-16461 None:intergenic 30.0%
!!! ATCTAGTTTTGAGCAATGAA+TGG - tig0039907:15763-15782 None:intergenic 30.0%
ACATGATAGATAGATATCCA+TGG + tig0039907:15971-15990 Msa1458910:exon 30.0%
TTATGTGTATGTTGCATAGT+TGG + tig0039907:16152-16171 Msa1458910:intron 30.0%
!!! GTTGCATAGTTGGTTTTAAA+AGG + tig0039907:16162-16181 Msa1458910:intron 30.0%
ACAGTAATATACTGTGATTC+TGG - tig0039907:16221-16240 None:intergenic 30.0%
! ATCCTCATTTTGTTGAAACT+TGG - tig0039907:16320-16339 None:intergenic 30.0%
ACTTAATTAACTGGTGAGTT+TGG - tig0039907:16358-16377 None:intergenic 30.0%
!! AGTTTGTTAACTATGTGTGT+AGG + tig0039907:16375-16394 Msa1458910:intron 30.0%
! GTTTGTTAACTATGTGTGTA+GGG + tig0039907:16376-16395 Msa1458910:intron 30.0%
TGTGTAGGGTTTATTATTTG+AGG + tig0039907:16390-16409 Msa1458910:intron 30.0%
!! TTACATTTGTTGTGACATGT+AGG - tig0039907:16442-16461 None:intergenic 30.0%
AATTGTGTAGAGAAAGGAGA+GGG - tig0039907:15710-15729 None:intergenic 35.0%
GGTAACAATTGTGTAGAGAA+AGG - tig0039907:15716-15735 None:intergenic 35.0%
!!! TTTTCTAGTTATGTGTTGGC+AGG - tig0039907:15737-15756 None:intergenic 35.0%
TGATGACCATTAACAAGATG+AGG - tig0039907:16016-16035 None:intergenic 35.0%
AGTGAATGAGGGAGAATAAA+GGG - tig0039907:16120-16139 None:intergenic 35.0%
GCAACATACACATAAAGCAA+AGG - tig0039907:16147-16166 None:intergenic 35.0%
CACCAAGTTTCAACAAAATG+AGG + tig0039907:16315-16334 Msa1458910:intron 35.0%
TTTGAGGAGAAAAACAGGTT+AGG + tig0039907:16406-16425 Msa1458910:intron 35.0%
AAAGAAATTACCACCTTCCA+TGG - tig0039907:16542-16561 None:intergenic 35.0%
CAAATTACAAGCCATTGAGT+TGG + tig0039907:16590-16609 Msa1458910:CDS 35.0%
AATTGTGTAGAGAAAGGAGA+GGG - tig0039907:15710-15729 None:intergenic 35.0%
GGTAACAATTGTGTAGAGAA+AGG - tig0039907:15716-15735 None:intergenic 35.0%
!!! TTTTCTAGTTATGTGTTGGC+AGG - tig0039907:15737-15756 None:intergenic 35.0%
TGATGACCATTAACAAGATG+AGG - tig0039907:16016-16035 None:intergenic 35.0%
AGTGAATGAGGGAGAATAAA+GGG - tig0039907:16120-16139 None:intergenic 35.0%
GCAACATACACATAAAGCAA+AGG - tig0039907:16147-16166 None:intergenic 35.0%
CACCAAGTTTCAACAAAATG+AGG + tig0039907:16315-16334 Msa1458910:intron 35.0%
TTTGAGGAGAAAAACAGGTT+AGG + tig0039907:16406-16425 Msa1458910:intron 35.0%
AAAGAAATTACCACCTTCCA+TGG - tig0039907:16542-16561 None:intergenic 35.0%
CAAATTACAAGCCATTGAGT+TGG + tig0039907:16590-16609 Msa1458910:CDS 35.0%
CAATTGTGTAGAGAAAGGAG+AGG - tig0039907:15711-15730 None:intergenic 40.0%
AAGAAACTGGACACAGTAGA+AGG - tig0039907:16052-16071 None:intergenic 40.0%
!! GGTTGATAGAAGCAAGAAAC+TGG - tig0039907:16065-16084 None:intergenic 40.0%
ACATTCTGCAATTCCTCCAA+GGG + tig0039907:16085-16104 Msa1458910:CDS 40.0%
GAGTGAATGAGGGAGAATAA+AGG - tig0039907:16121-16140 None:intergenic 40.0%
GCAAAGGAAAGAGTGAATGA+GGG - tig0039907:16131-16150 None:intergenic 40.0%
AGCAAAGGAAAGAGTGAATG+AGG - tig0039907:16132-16151 None:intergenic 40.0%
TGGTGAGTTTGGCAAAATCA+AGG - tig0039907:16347-16366 None:intergenic 40.0%
!! CATTTGTTGTGACATGTAGG+TGG - tig0039907:16439-16458 None:intergenic 40.0%
TAACCAATGTCATCCATGCA+TGG + tig0039907:16459-16478 Msa1458910:intron 40.0%
GGTGGTAATTTCTTTCTCCA+AGG + tig0039907:16547-16566 Msa1458910:CDS 40.0%
CAATGGCATTTCCAACTCAA+TGG - tig0039907:16604-16623 None:intergenic 40.0%
!! TTGAGTTGGAAATGCCATTG+TGG + tig0039907:16604-16623 Msa1458910:CDS 40.0%
CAATTGTGTAGAGAAAGGAG+AGG - tig0039907:15711-15730 None:intergenic 40.0%
AAGAAACTGGACACAGTAGA+AGG - tig0039907:16052-16071 None:intergenic 40.0%
!! GGTTGATAGAAGCAAGAAAC+TGG - tig0039907:16065-16084 None:intergenic 40.0%
ACATTCTGCAATTCCTCCAA+GGG + tig0039907:16085-16104 Msa1458910:CDS 40.0%
GAGTGAATGAGGGAGAATAA+AGG - tig0039907:16121-16140 None:intergenic 40.0%
GCAAAGGAAAGAGTGAATGA+GGG - tig0039907:16131-16150 None:intergenic 40.0%
AGCAAAGGAAAGAGTGAATG+AGG - tig0039907:16132-16151 None:intergenic 40.0%
TGGTGAGTTTGGCAAAATCA+AGG - tig0039907:16347-16366 None:intergenic 40.0%
!! CATTTGTTGTGACATGTAGG+TGG - tig0039907:16439-16458 None:intergenic 40.0%
TAACCAATGTCATCCATGCA+TGG + tig0039907:16459-16478 Msa1458910:intron 40.0%
GGTGGTAATTTCTTTCTCCA+AGG + tig0039907:16547-16566 Msa1458910:CDS 40.0%
CAATGGCATTTCCAACTCAA+TGG - tig0039907:16604-16623 None:intergenic 40.0%
!! TTGAGTTGGAAATGCCATTG+TGG + tig0039907:16604-16623 Msa1458910:CDS 40.0%
AAAGGAGAGGGATAGTGTAG+TGG - tig0039907:15698-15717 None:intergenic 45.0%
CTTGGAGGAATTGCAGAATG+TGG - tig0039907:16086-16105 None:intergenic 45.0%
CACATTCTGCAATTCCTCCA+AGG + tig0039907:16084-16103 Msa1458910:CDS 45.0%
TAAAGGGAAAAGCCTACCCT+TGG - tig0039907:16104-16123 None:intergenic 45.0%
GGAATCGGTGCATGTTTGAA+TGG + tig0039907:16183-16202 Msa1458910:intron 45.0%
TCGGTGCATGTTTGAATGGA+CGG + tig0039907:16187-16206 Msa1458910:intron 45.0%
GCTCGGAGCTTCAACAAAAT+CGG - tig0039907:16270-16289 None:intergenic 45.0%
ACCTTCCATGGATGAAGTTG+AGG - tig0039907:16530-16549 None:intergenic 45.0%
TCAACTTCATCCATGGAAGG+TGG + tig0039907:16529-16548 Msa1458910:CDS 45.0%
AGTTGGAAATGCCATTGTGG+TGG + tig0039907:16607-16626 Msa1458910:CDS 45.0%
AAAGGAGAGGGATAGTGTAG+TGG - tig0039907:15698-15717 None:intergenic 45.0%
CTTGGAGGAATTGCAGAATG+TGG - tig0039907:16086-16105 None:intergenic 45.0%
CACATTCTGCAATTCCTCCA+AGG + tig0039907:16084-16103 Msa1458910:CDS 45.0%
TAAAGGGAAAAGCCTACCCT+TGG - tig0039907:16104-16123 None:intergenic 45.0%
GGAATCGGTGCATGTTTGAA+TGG + tig0039907:16183-16202 Msa1458910:intron 45.0%
TCGGTGCATGTTTGAATGGA+CGG + tig0039907:16187-16206 Msa1458910:intron 45.0%
GCTCGGAGCTTCAACAAAAT+CGG - tig0039907:16270-16289 None:intergenic 45.0%
ACCTTCCATGGATGAAGTTG+AGG - tig0039907:16530-16549 None:intergenic 45.0%
TCAACTTCATCCATGGAAGG+TGG + tig0039907:16529-16548 Msa1458910:CDS 45.0%
AGTTGGAAATGCCATTGTGG+TGG + tig0039907:16607-16626 Msa1458910:CDS 45.0%
! AGATAGAGGGTGCAGAGTGT+TGG - tig0039907:15669-15688 None:intergenic 50.0%
! GTAGTGGAGGCTGAGATAGA+GGG - tig0039907:15682-15701 None:intergenic 50.0%
! TGTAGTGGAGGCTGAGATAG+AGG - tig0039907:15683-15702 None:intergenic 50.0%
TCTGCAATTCCTCCAAGGGT+AGG + tig0039907:16089-16108 Msa1458910:intron 50.0%
CTGCCATGCATGGATGACAT+TGG - tig0039907:16465-16484 None:intergenic 50.0%
GGCTGAACATGTTCATGGCT+TGG - tig0039907:16496-16515 None:intergenic 50.0%
AGCCATGAACATGTTCAGCC+AGG + tig0039907:16496-16515 Msa1458910:intron 50.0%
GACCTGGCTGAACATGTTCA+TGG - tig0039907:16501-16520 None:intergenic 50.0%
CACTGCCTCAACTTCATCCA+TGG + tig0039907:16522-16541 Msa1458910:CDS 50.0%
GCCTCAACTTCATCCATGGA+AGG + tig0039907:16526-16545 Msa1458910:CDS 50.0%
TGAAGAAGTGGCCACCACAA+TGG - tig0039907:16621-16640 None:intergenic 50.0%
! AGATAGAGGGTGCAGAGTGT+TGG - tig0039907:15669-15688 None:intergenic 50.0%
! GTAGTGGAGGCTGAGATAGA+GGG - tig0039907:15682-15701 None:intergenic 50.0%
! TGTAGTGGAGGCTGAGATAG+AGG - tig0039907:15683-15702 None:intergenic 50.0%
TCTGCAATTCCTCCAAGGGT+AGG + tig0039907:16089-16108 Msa1458910:intron 50.0%
CTGCCATGCATGGATGACAT+TGG - tig0039907:16465-16484 None:intergenic 50.0%
GGCTGAACATGTTCATGGCT+TGG - tig0039907:16496-16515 None:intergenic 50.0%
AGCCATGAACATGTTCAGCC+AGG + tig0039907:16496-16515 Msa1458910:intron 50.0%
GACCTGGCTGAACATGTTCA+TGG - tig0039907:16501-16520 None:intergenic 50.0%
CACTGCCTCAACTTCATCCA+TGG + tig0039907:16522-16541 Msa1458910:CDS 50.0%
GCCTCAACTTCATCCATGGA+AGG + tig0039907:16526-16545 Msa1458910:CDS 50.0%
TGAAGAAGTGGCCACCACAA+TGG - tig0039907:16621-16640 None:intergenic 50.0%
GGAGAGGGATAGTGTAGTGG+AGG - tig0039907:15695-15714 None:intergenic 55.0%
AGGGAAAAGCCTACCCTTGG+AGG - tig0039907:16101-16120 None:intergenic 55.0%
GGAACTTGCACTGCCATGCA+TGG - tig0039907:16475-16494 None:intergenic 55.0%
GAAGTTGAGGCAGTGTGACC+TGG - tig0039907:16517-16536 None:intergenic 55.0%
GGAGAGGGATAGTGTAGTGG+AGG - tig0039907:15695-15714 None:intergenic 55.0%
AGGGAAAAGCCTACCCTTGG+AGG - tig0039907:16101-16120 None:intergenic 55.0%
GGAACTTGCACTGCCATGCA+TGG - tig0039907:16475-16494 None:intergenic 55.0%
GAAGTTGAGGCAGTGTGACC+TGG - tig0039907:16517-16536 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0039907 gene 15653 16648 15653 ID=Msa1458910;Name=Msa1458910
tig0039907 mRNA 15653 16648 15653 ID=Msa1458910-mRNA-1;Parent=Msa1458910;Name=Msa1458910-mRNA-1;_AED=0.16;_eAED=0.17;_QI=337|0|0.5|1|0|0|2|0|83
tig0039907 exon 15653 16106 15653 ID=Msa1458910-mRNA-1:exon:21999;Parent=Msa1458910-mRNA-1
tig0039907 exon 16514 16648 16514 ID=Msa1458910-mRNA-1:exon:22000;Parent=Msa1458910-mRNA-1
tig0039907 five_prime_UTR 15653 15989 15653 ID=Msa1458910-mRNA-1:five_prime_utr;Parent=Msa1458910-mRNA-1
tig0039907 CDS 15990 16106 15990 ID=Msa1458910-mRNA-1:cds;Parent=Msa1458910-mRNA-1
tig0039907 CDS 16514 16648 16514 ID=Msa1458910-mRNA-1:cds;Parent=Msa1458910-mRNA-1
Gene Sequence

>Msa1458910

ATGGCTTCATTTAATTCATATAACCTCATCTTGTTAATGGTCATCATATTTATACTTCACCTTCTACTGTGTCCAGTTTCTTGCTTCTATCAACCACATTCTGCAATTCCTCCAAGGCCAGGTCACACTGCCTCAACTTCATCCATGGAAGGTGGTAATTTCTTTCTCCAAGGTAATAATAATAACGGATACTCAAATTACAAGCCATTGAGTTGGAAATGCCATTGTGGTGGCCACTTCTTCAACCCTTAG

Protein sequence

>Msa1458910

MASFNSYNLILLMVIIFILHLLLCPVSCFYQPHSAIPPRPGHTASTSSMEGGNFFLQGNNNNGYSNYKPLSWKCHCGGHFFNP*