Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1177400 | A0A396GM00 | 81.579 | 152 | 11 | 1 | 1 | 152 | 6 | 140 | 1.25e-82 | 249 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0739480 | Msa1177400 | 0.806993 | 6.281004e-50 | -8.615850e-47 |
| Msa0742180 | Msa1177400 | 0.842444 | 2.575925e-58 | -8.615850e-47 |
| Msa0786040 | Msa1177400 | 0.830410 | 2.983741e-55 | -8.615850e-47 |
| Msa0823400 | Msa1177400 | 0.834335 | 3.180415e-56 | -8.615850e-47 |
| Msa0176470 | Msa1177400 | 0.807515 | 4.868932e-50 | -8.615850e-47 |
| Msa0194380 | Msa1177400 | 0.808188 | 3.501446e-50 | -8.615850e-47 |
| Msa0206800 | Msa1177400 | 0.815166 | 1.062296e-51 | -8.615850e-47 |
| Msa0373960 | Msa1177400 | 0.826685 | 2.369071e-54 | -8.615850e-47 |
| Msa0423100 | Msa1177400 | 0.801333 | 9.461165e-49 | -8.615850e-47 |
| Msa0441590 | Msa1177400 | 0.809968 | 1.455628e-50 | -8.615850e-47 |
| Msa0469260 | Msa1177400 | 0.804262 | 2.350156e-49 | -8.615850e-47 |
| Msa0996270 | Msa1177400 | 0.820626 | 6.211167e-53 | -8.615850e-47 |
| Msa0999340 | Msa1177400 | 0.816211 | 6.214714e-52 | -8.615850e-47 |
| Msa0999350 | Msa1177400 | 0.812329 | 4.478816e-51 | -8.615850e-47 |
| Msa0999390 | Msa1177400 | 0.809848 | 1.544506e-50 | -8.615850e-47 |
| Msa0999400 | Msa1177400 | 0.801804 | 7.574838e-49 | -8.615850e-47 |
| Msa1092020 | Msa1177400 | 0.807470 | 4.976860e-50 | -8.615850e-47 |
| Msa0277290 | Msa1177400 | 0.813584 | 2.377064e-51 | -8.615850e-47 |
| Msa0351770 | Msa1177400 | 0.820287 | 7.428553e-53 | -8.615850e-47 |
| Msa1135380 | Msa1177400 | 0.802098 | 6.589899e-49 | -8.615850e-47 |
| Msa1177400 | Msa1228190 | 0.804857 | 1.766070e-49 | -8.615850e-47 |
| Msa1177400 | Msa1317170 | 0.858055 | 1.071237e-62 | -8.615850e-47 |
| Msa1177400 | Msa1345420 | 0.823765 | 1.161203e-53 | -8.615850e-47 |
| Msa1177400 | Msa1435480 | 0.819040 | 1.430609e-52 | -8.615850e-47 |
| Msa1177400 | Msa1462530 | 0.809533 | 1.805113e-50 | -8.615850e-47 |
| Msa0591800 | Msa1177400 | 0.846324 | 2.333432e-59 | -8.615850e-47 |
| Msa0860170 | Msa1177400 | 0.815129 | 1.083118e-51 | -8.615850e-47 |
| Msa0927120 | Msa1177400 | 0.825902 | 3.639242e-54 | -8.615850e-47 |
| Msa0946210 | Msa1177400 | 0.804247 | 2.367771e-49 | -8.615850e-47 |
| Msa0961560 | Msa1177400 | 0.807009 | 6.229906e-50 | -8.615850e-47 |
| Msa0965310 | Msa1177400 | 0.805113 | 1.561741e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1177400 | MtrunA17_Chr8g0347351 | 81.579 | 152 | 11 | 1 | 1 | 152 | 6 | 140 | 2.42e-86 | 249 |
| Msa1177400 | MtrunA17_Chr8g0347371 | 33.117 | 154 | 74 | 5 | 1 | 153 | 1 | 126 | 1.20e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1177400 | AT3G61660.2 | 55.556 | 54 | 20 | 1 | 8 | 61 | 2 | 51 | 5.45e-13 | 62.8 |
| Msa1177400 | AT3G61660.1 | 64.103 | 39 | 14 | 0 | 23 | 61 | 3 | 41 | 2.11e-12 | 60.8 |
Find 23 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTTGGGTCACAAGCTTT+AGG | 0.271752 | 8_1:-9784350 | Msa1177400:CDS |
| AGTTCATATGAATCATATAA+AGG | 0.353897 | 8_1:+9784552 | None:intergenic |
| AGAAAGCAAAAGAGTCTTCT+TGG | 0.367922 | 8_1:-9784365 | Msa1177400:CDS |
| CTCTTGATCATCACATCAAC+AGG | 0.370624 | 8_1:-9784524 | Msa1177400:CDS |
| TTTATATGATTCATATGAAC+TGG | 0.406641 | 8_1:-9784550 | Msa1177400:CDS |
| GATTTGATCATCACATCAAC+AGG | 0.421007 | 8_1:-9784473 | Msa1177400:CDS |
| GTTCATATGAATCATATAAA+GGG | 0.444445 | 8_1:+9784553 | None:intergenic |
| TATGCATGATTCTCATGATA+TGG | 0.452143 | 8_1:-9784397 | Msa1177400:CDS |
| GATGAAGATGATGAAGAAGA+AGG | 0.460484 | 8_1:-9784319 | Msa1177400:CDS |
| TCACATCAACAGGAATCTAA+TGG | 0.461388 | 8_1:-9784463 | Msa1177400:CDS |
| TCACATCAACAGGAATCTAA+TGG | 0.461388 | 8_1:-9784514 | Msa1177400:CDS |
| AGATGAACAAGAAGCATTGA+AGG | 0.464858 | 8_1:-9784631 | Msa1177400:CDS |
| GAAAGCAAAAGAGTCTTCTT+GGG | 0.496086 | 8_1:-9784364 | Msa1177400:CDS |
| CAAGGAAATGAGAAGAGGTA+TGG | 0.543597 | 8_1:-9784268 | Msa1177400:CDS |
| ATGGCATTTCCATCTCTCCA+TGG | 0.560072 | 8_1:-9784495 | Msa1177400:CDS |
| TGATCAAATCCATGGAGAGA+TGG | 0.564729 | 8_1:+9784486 | None:intergenic |
| AAGAACAAGGAAATGAGAAG+AGG | 0.574484 | 8_1:-9784273 | Msa1177400:CDS |
| ATGGCATTTCCATCTCTCCA+TGG | 0.584910 | 8_1:-9784444 | Msa1177400:CDS |
| GCAAGGGCAATGTGGGATTG+TGG | 0.585400 | 8_1:-9784579 | Msa1177400:CDS |
| GATGAACAAGAAGCATTGAA+GGG | 0.592575 | 8_1:-9784630 | Msa1177400:CDS |
| AAATGAGAAGAGGTATGGCT+AGG | 0.594302 | 8_1:-9784263 | Msa1177400:CDS |
| ATGAAGAAAACCAACATGAG+TGG | 0.609953 | 8_1:-9784669 | None:intergenic |
| TGATGTGATGATCAAATCCA+TGG | 0.613524 | 8_1:+9784478 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTCATATGAATCATATAAA+GGG | + | chr8_1:9784319-9784338 | None:intergenic | 20.0% |
| !! | AGTTCATATGAATCATATAA+AGG | + | chr8_1:9784320-9784339 | None:intergenic | 20.0% |
| !! | TTTATATGATTCATATGAAC+TGG | - | chr8_1:9784319-9784338 | Msa1177400:CDS | 20.0% |
| ! | AGAAAAACTAAAGAAGAACA+AGG | - | chr8_1:9784583-9784602 | Msa1177400:CDS | 25.0% |
| !! | GTAAATTTTCTTTCTTGTGA+AGG | - | chr8_1:9784635-9784654 | Msa1177400:CDS | 25.0% |
| AGAAAGAAGAAGAAAAAGCA+AGG | - | chr8_1:9784273-9784292 | Msa1177400:CDS | 30.0% | |
| GAAAGAAGAAGAAAAAGCAA+GGG | - | chr8_1:9784274-9784293 | Msa1177400:CDS | 30.0% | |
| TATGCATGATTCTCATGATA+TGG | - | chr8_1:9784472-9784491 | Msa1177400:CDS | 30.0% | |
| ! | ACTCTTTTGCTTTCTCTATA+TGG | + | chr8_1:9784499-9784518 | None:intergenic | 30.0% |
| !! | AGATGAACAAGAAGCATTGA+AGG | - | chr8_1:9784238-9784257 | Msa1177400:CDS | 35.0% |
| !! | GATGAACAAGAAGCATTGAA+GGG | - | chr8_1:9784239-9784258 | Msa1177400:CDS | 35.0% |
| TCACATCAACAGGAATCTAA+TGG | - | chr8_1:9784355-9784374 | Msa1177400:CDS | 35.0% | |
| TGATGTGATGATCAAATCCA+TGG | + | chr8_1:9784394-9784413 | None:intergenic | 35.0% | |
| GATTTGATCATCACATCAAC+AGG | - | chr8_1:9784396-9784415 | Msa1177400:CDS | 35.0% | |
| TCACATCAACAGGAATCTAA+TGG | - | chr8_1:9784355-9784374 | Msa1177400:CDS | 35.0% | |
| AGAAAGCAAAAGAGTCTTCT+TGG | - | chr8_1:9784504-9784523 | Msa1177400:CDS | 35.0% | |
| GAAAGCAAAAGAGTCTTCTT+GGG | - | chr8_1:9784505-9784524 | Msa1177400:CDS | 35.0% | |
| GATGAAGATGATGAAGAAGA+AGG | - | chr8_1:9784550-9784569 | Msa1177400:CDS | 35.0% | |
| AAGAACAAGGAAATGAGAAG+AGG | - | chr8_1:9784596-9784615 | Msa1177400:CDS | 35.0% | |
| AAGAAAAAGCAAGGGCAATG+TGG | - | chr8_1:9784282-9784301 | Msa1177400:CDS | 40.0% | |
| AGAAAAAGCAAGGGCAATGT+GGG | - | chr8_1:9784283-9784302 | Msa1177400:CDS | 40.0% | |
| CTCTTGATCATCACATCAAC+AGG | - | chr8_1:9784345-9784364 | Msa1177400:CDS | 40.0% | |
| TGATCAAATCCATGGAGAGA+TGG | + | chr8_1:9784386-9784405 | None:intergenic | 40.0% | |
| !! | CGATTATGCGTTTTGATCCA+TGG | + | chr8_1:9784445-9784464 | None:intergenic | 40.0% |
| CAAGGAAATGAGAAGAGGTA+TGG | - | chr8_1:9784601-9784620 | Msa1177400:CDS | 40.0% | |
| ! | AAATGAGAAGAGGTATGGCT+AGG | - | chr8_1:9784606-9784625 | Msa1177400:CDS | 40.0% |
| ATGGCATTTCCATCTCTCCA+TGG | - | chr8_1:9784374-9784393 | Msa1177400:CDS | 45.0% | |
| ATGGCATTTCCATCTCTCCA+TGG | - | chr8_1:9784374-9784393 | Msa1177400:CDS | 45.0% | |
| !! | CGTTTTGATCCATGGAGAGA+TGG | + | chr8_1:9784437-9784456 | None:intergenic | 45.0% |
| CTTCTTGGGTCACAAGCTTT+AGG | - | chr8_1:9784519-9784538 | Msa1177400:CDS | 45.0% | |
| GCAAGGGCAATGTGGGATTG+TGG | - | chr8_1:9784290-9784309 | Msa1177400:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 9784215 | 9784676 | 9784215 | ID=Msa1177400;Name=Msa1177400 |
| chr8_1 | mRNA | 9784215 | 9784676 | 9784215 | ID=Msa1177400-mRNA-1;Parent=Msa1177400;Name=Msa1177400-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|153 |
| chr8_1 | exon | 9784215 | 9784676 | 9784215 | ID=Msa1177400-mRNA-1:exon:3394;Parent=Msa1177400-mRNA-1 |
| chr8_1 | CDS | 9784215 | 9784676 | 9784215 | ID=Msa1177400-mRNA-1:cds;Parent=Msa1177400-mRNA-1 |
| Gene Sequence |
| Protein sequence |
