Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0373960 | AES72186.1 | 92.623 | 122 | 8 | 1 | 1 | 122 | 1 | 121 | 5.53e-74 | 226 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0373960 | sp|Q9LFT5|EPFL1_ARATH | 47.573 | 103 | 44 | 3 | 26 | 122 | 24 | 122 | 2.12e-24 | 93.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0373960 | G7J3Q1 | 92.623 | 122 | 8 | 1 | 1 | 122 | 1 | 121 | 2.64e-74 | 226 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002930 | Msa0373960 | 0.841454 | 4.705381e-58 | -8.615850e-47 |
| Msa0015710 | Msa0373960 | 0.833576 | 4.927487e-56 | -8.615850e-47 |
| Msa0029910 | Msa0373960 | 0.802671 | 5.021759e-49 | -8.615850e-47 |
| Msa0104500 | Msa0373960 | 0.820306 | 7.353450e-53 | -8.615850e-47 |
| Msa0106790 | Msa0373960 | 0.801535 | 8.602771e-49 | -8.615850e-47 |
| Msa0109880 | Msa0373960 | 0.808712 | 2.706825e-50 | -8.615850e-47 |
| Msa0124130 | Msa0373960 | 0.833297 | 5.781502e-56 | -8.615850e-47 |
| Msa0138290 | Msa0373960 | 0.810749 | 9.875121e-51 | -8.615850e-47 |
| Msa0149140 | Msa0373960 | 0.802949 | 4.400988e-49 | -8.615850e-47 |
| Msa0152500 | Msa0373960 | 0.810866 | 9.315779e-51 | -8.615850e-47 |
| Msa0153420 | Msa0373960 | 0.804337 | 2.267629e-49 | -8.615850e-47 |
| Msa0156250 | Msa0373960 | 0.819235 | 1.292023e-52 | -8.615850e-47 |
| Msa0176470 | Msa0373960 | 0.830460 | 2.900936e-55 | -8.615850e-47 |
| Msa0191040 | Msa0373960 | 0.815203 | 1.042652e-51 | -8.615850e-47 |
| Msa0194380 | Msa0373960 | 0.854301 | 1.348591e-61 | -8.615850e-47 |
| Msa0203030 | Msa0373960 | 0.811708 | 6.115018e-51 | -8.615850e-47 |
| Msa0206800 | Msa0373960 | 0.833478 | 5.212167e-56 | -8.615850e-47 |
| Msa0212150 | Msa0373960 | 0.815666 | 8.223930e-52 | -8.615850e-47 |
| Msa0219190 | Msa0373960 | 0.811162 | 8.036218e-51 | -8.615850e-47 |
| Msa0233340 | Msa0373960 | 0.814313 | 1.641956e-51 | -8.615850e-47 |
| Msa0236110 | Msa0373960 | 0.828752 | 7.547282e-55 | -8.615850e-47 |
| Msa0373960 | Msa0382010 | 0.820995 | 5.106994e-53 | -8.615850e-47 |
| Msa0373960 | Msa0406000 | 0.849159 | 3.867089e-60 | -8.615850e-47 |
| Msa0373960 | Msa0423100 | 0.923312 | 3.136762e-89 | -8.615850e-47 |
| Msa0373960 | Msa0424330 | 0.810117 | 1.351886e-50 | -8.615850e-47 |
| Msa0373960 | Msa0424340 | 0.824067 | 9.863410e-54 | -8.615850e-47 |
| Msa0373960 | Msa0436500 | 0.811999 | 5.286603e-51 | -8.615850e-47 |
| Msa0373960 | Msa0451400 | 0.850210 | 1.967069e-60 | -8.615850e-47 |
| Msa0373960 | Msa0469260 | 0.904305 | 1.410725e-79 | -8.615850e-47 |
| Msa0373960 | Msa0476590 | 0.803803 | 2.928903e-49 | -8.615850e-47 |
| Msa0373960 | Msa0492190 | 0.847023 | 1.503012e-59 | -8.615850e-47 |
| Msa0373960 | Msa0514980 | 0.812021 | 5.227408e-51 | -8.615850e-47 |
| Msa0373960 | Msa0515950 | 0.808309 | 3.299798e-50 | -8.615850e-47 |
| Msa0373960 | Msa0520510 | 0.857697 | 1.368368e-62 | -8.615850e-47 |
| Msa0373960 | Msa0534930 | 0.813382 | 2.633018e-51 | -8.615850e-47 |
| Msa0373960 | Msa0535040 | 0.814217 | 1.724238e-51 | -8.615850e-47 |
| Msa0373960 | Msa0546710 | 0.800631 | 1.316632e-48 | -8.615850e-47 |
| Msa0373960 | Msa0575740 | 0.851112 | 1.097178e-60 | -8.615850e-47 |
| Msa0373960 | Msa0591790 | 0.827932 | 1.190158e-54 | -8.615850e-47 |
| Msa0373960 | Msa0591800 | 0.872603 | 2.802150e-67 | -8.615850e-47 |
| Msa0373960 | Msa0594090 | 0.804200 | 2.421190e-49 | -8.615850e-47 |
| Msa0373960 | Msa0639460 | 0.807129 | 5.875969e-50 | -8.615850e-47 |
| Msa0373960 | Msa0641360 | 0.808027 | 3.789656e-50 | -8.615850e-47 |
| Msa0373960 | Msa0667850 | 0.856703 | 2.688815e-62 | -8.615850e-47 |
| Msa0373960 | Msa0669840 | 0.842675 | 2.237419e-58 | -8.615850e-47 |
| Msa0373960 | Msa0682360 | 0.805395 | 1.362857e-49 | -8.615850e-47 |
| Msa0373960 | Msa0711320 | 0.800687 | 1.282492e-48 | -8.615850e-47 |
| Msa0373960 | Msa0725860 | 0.832691 | 8.182198e-56 | -8.615850e-47 |
| Msa0373960 | Msa0739480 | 0.819474 | 1.139624e-52 | -8.615850e-47 |
| Msa0373960 | Msa0739580 | 0.812075 | 5.087540e-51 | -8.615850e-47 |
| Msa0373960 | Msa0742180 | 0.864317 | 1.326718e-64 | -8.615850e-47 |
| Msa0373960 | Msa0786040 | 0.840029 | 1.111946e-57 | -8.615850e-47 |
| Msa0373960 | Msa0823400 | 0.884826 | 1.375769e-71 | -8.615850e-47 |
| Msa0373960 | Msa0828830 | 0.803031 | 4.231353e-49 | -8.615850e-47 |
| Msa0373960 | Msa0845940 | 0.827181 | 1.802343e-54 | -8.615850e-47 |
| Msa0373960 | Msa0860170 | 0.853020 | 3.149214e-61 | -8.615850e-47 |
| Msa0373960 | Msa0860280 | 0.820872 | 5.453194e-53 | -8.615850e-47 |
| Msa0373960 | Msa0862310 | 0.862147 | 6.224058e-64 | -8.615850e-47 |
| Msa0373960 | Msa0885060 | 0.839370 | 1.650604e-57 | -8.615850e-47 |
| Msa0373960 | Msa0896350 | 0.801818 | 7.525702e-49 | -8.615850e-47 |
| Msa0373960 | Msa0899950 | 0.861806 | 7.919787e-64 | -8.615850e-47 |
| Msa0373960 | Msa0922750 | 0.824574 | 7.497118e-54 | -8.615850e-47 |
| Msa0373960 | Msa0927120 | 0.840593 | 7.918119e-58 | -8.615850e-47 |
| Msa0373960 | Msa0929870 | 0.846960 | 1.564081e-59 | -8.615850e-47 |
| Msa0373960 | Msa0941820 | 0.824771 | 6.739031e-54 | -8.615850e-47 |
| Msa0373960 | Msa0961560 | 0.851568 | 8.155483e-61 | -8.615850e-47 |
| Msa0373960 | Msa0965310 | 0.877082 | 8.354863e-69 | -8.615850e-47 |
| Msa0373960 | Msa0973250 | 0.800391 | 1.473340e-48 | -8.615850e-47 |
| Msa0373960 | Msa0979510 | 0.839727 | 1.333137e-57 | -8.615850e-47 |
| Msa0373960 | Msa0996270 | 0.873275 | 1.668114e-67 | -8.615850e-47 |
| Msa0373960 | Msa0999300 | 0.831653 | 1.477529e-55 | -8.615850e-47 |
| Msa0373960 | Msa0999340 | 0.877541 | 5.785806e-69 | -8.615850e-47 |
| Msa0373960 | Msa0999350 | 0.888939 | 3.783852e-73 | -8.615850e-47 |
| Msa0373960 | Msa0999360 | 0.850234 | 1.937009e-60 | -8.615850e-47 |
| Msa0373960 | Msa0999390 | 0.871004 | 9.507565e-67 | -8.615850e-47 |
| Msa0373960 | Msa0999400 | 0.880137 | 7.028001e-70 | -8.615850e-47 |
| Msa0373960 | Msa0999410 | 0.852367 | 4.835273e-61 | -8.615850e-47 |
| Msa0373960 | Msa0999700 | 0.858551 | 7.623992e-63 | -8.615850e-47 |
| Msa0373960 | Msa1034680 | 0.806578 | 7.686534e-50 | -8.615850e-47 |
| Msa0373960 | Msa1060580 | 0.812633 | 3.843897e-51 | -8.615850e-47 |
| Msa0373960 | Msa1073340 | 0.833680 | 4.640084e-56 | -8.615850e-47 |
| Msa0373960 | Msa1084520 | 0.807278 | 5.465680e-50 | -8.615850e-47 |
| Msa0373960 | Msa1092020 | 0.835964 | 1.235022e-56 | -8.615850e-47 |
| Msa0373960 | Msa1100140 | 0.808462 | 3.061171e-50 | -8.615850e-47 |
| Msa0373960 | Msa1113850 | 0.807723 | 4.397465e-50 | -8.615850e-47 |
| Msa0373960 | Msa1125810 | 0.814074 | 1.854283e-51 | -8.615850e-47 |
| Msa0373960 | Msa1135380 | 0.825569 | 4.364389e-54 | -8.615850e-47 |
| Msa0373960 | Msa1145020 | 0.861767 | 8.140899e-64 | -8.615850e-47 |
| Msa0373960 | Msa1171310 | 0.810893 | 9.192077e-51 | -8.615850e-47 |
| Msa0373960 | Msa1176580 | 0.801734 | 7.827913e-49 | -8.615850e-47 |
| Msa0373960 | Msa1177400 | 0.826685 | 2.369071e-54 | -8.615850e-47 |
| Msa0373960 | Msa1179360 | 0.824187 | 9.247099e-54 | -8.615850e-47 |
| Msa0373960 | Msa1179520 | 0.841441 | 4.744191e-58 | -8.615850e-47 |
| Msa0373960 | Msa1201110 | 0.819611 | 1.060736e-52 | -8.615850e-47 |
| Msa0373960 | Msa1201160 | 0.839914 | 1.191331e-57 | -8.615850e-47 |
| Msa0373960 | Msa1228190 | 0.882758 | 7.962144e-71 | -8.615850e-47 |
| Msa0373960 | Msa1233560 | 0.805356 | 1.388703e-49 | -8.615850e-47 |
| Msa0373960 | Msa1271260 | 0.880794 | 4.091859e-70 | -8.615850e-47 |
| Msa0373960 | Msa1292010 | 0.825544 | 4.423430e-54 | -8.615850e-47 |
| Msa0373960 | Msa1294290 | 0.828255 | 9.953082e-55 | -8.615850e-47 |
| Msa0373960 | Msa1305510 | 0.801617 | 8.274083e-49 | -8.615850e-47 |
| Msa0373960 | Msa1316540 | 0.805871 | 1.082582e-49 | -8.615850e-47 |
| Msa0373960 | Msa1317170 | 0.802705 | 4.942753e-49 | -8.615850e-47 |
| Msa0373960 | Msa1339440 | 0.843670 | 1.214741e-58 | -8.615850e-47 |
| Msa0373960 | Msa1345420 | 0.854200 | 1.441568e-61 | -8.615850e-47 |
| Msa0373960 | Msa1378770 | 0.801513 | 8.690962e-49 | -8.615850e-47 |
| Msa0373960 | Msa1379970 | 0.849548 | 3.013171e-60 | -8.615850e-47 |
| Msa0373960 | Msa1388770 | 0.803603 | 3.221686e-49 | -8.615850e-47 |
| Msa0373960 | Msa1389050 | 0.871809 | 5.148195e-67 | -8.615850e-47 |
| Msa0373960 | Msa1406780 | 0.827307 | 1.681310e-54 | -8.615850e-47 |
| Msa0373960 | Msa1420060 | 0.814101 | 1.828354e-51 | -8.615850e-47 |
| Msa0373960 | Msa1421550 | 0.836775 | 7.681174e-57 | -8.615850e-47 |
| Msa0373960 | Msa1431850 | 0.836127 | 1.122888e-56 | -8.615850e-47 |
| Msa0373960 | Msa1435480 | 0.920111 | 1.935135e-87 | -8.615850e-47 |
| Msa0373960 | Msa1442630 | 0.805752 | 1.146820e-49 | -8.615850e-47 |
| Msa0373960 | Msa1458910 | 0.916595 | 1.474176e-85 | -8.615850e-47 |
| Msa0373960 | Msa1462530 | 0.834605 | 2.721528e-56 | -8.615850e-47 |
| Msa0242540 | Msa0373960 | 0.801662 | 8.099514e-49 | -8.615850e-47 |
| Msa0251240 | Msa0373960 | 0.808362 | 3.214995e-50 | -8.615850e-47 |
| Msa0252630 | Msa0373960 | 0.822788 | 1.963678e-53 | -8.615850e-47 |
| Msa0252770 | Msa0373960 | 0.819945 | 8.895844e-53 | -8.615850e-47 |
| Msa0273990 | Msa0373960 | 0.819146 | 1.353289e-52 | -8.615850e-47 |
| Msa0277290 | Msa0373960 | 0.820826 | 5.587655e-53 | -8.615850e-47 |
| Msa0280750 | Msa0373960 | 0.834836 | 2.380438e-56 | -8.615850e-47 |
| Msa0288190 | Msa0373960 | 0.836730 | 7.884660e-57 | -8.615850e-47 |
| Msa0288200 | Msa0373960 | 0.832438 | 9.452483e-56 | -8.615850e-47 |
| Msa0289920 | Msa0373960 | 0.808433 | 3.104289e-50 | -8.615850e-47 |
| Msa0291330 | Msa0373960 | 0.826204 | 3.084899e-54 | -8.615850e-47 |
| Msa0298240 | Msa0373960 | 0.818580 | 1.820220e-52 | -8.615850e-47 |
| Msa0306120 | Msa0373960 | 0.819355 | 1.212917e-52 | -8.615850e-47 |
| Msa0311690 | Msa0373960 | 0.821261 | 4.434976e-53 | -8.615850e-47 |
| Msa0314630 | Msa0373960 | 0.831386 | 1.719516e-55 | -8.615850e-47 |
| Msa0322830 | Msa0373960 | 0.828688 | 7.822801e-55 | -8.615850e-47 |
| Msa0322840 | Msa0373960 | 0.842990 | 1.844836e-58 | -8.615850e-47 |
| Msa0322860 | Msa0373960 | 0.829792 | 4.222703e-55 | -8.615850e-47 |
| Msa0326540 | Msa0373960 | 0.814832 | 1.260352e-51 | -8.615850e-47 |
| Msa0329460 | Msa0373960 | 0.800319 | 1.523931e-48 | -8.615850e-47 |
| Msa0329620 | Msa0373960 | 0.847895 | 8.658676e-60 | -8.615850e-47 |
| Msa0351770 | Msa0373960 | 0.811539 | 6.654427e-51 | -8.615850e-47 |
| Msa0359250 | Msa0373960 | 0.833240 | 5.974758e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0373960 | MtrunA17_Chr3g0123851 | 92.623 | 122 | 8 | 1 | 1 | 122 | 1 | 121 | 5.08e-78 | 226 |
| Msa0373960 | MtrunA17_Chr8g0381891 | 57.143 | 126 | 47 | 3 | 1 | 122 | 1 | 123 | 8.39e-35 | 116 |
| Msa0373960 | MtrunA17_Chr3g0140691 | 41.860 | 86 | 38 | 2 | 40 | 122 | 22 | 98 | 3.68e-16 | 68.6 |
| Msa0373960 | MtrunA17_Chr4g0054461 | 42.857 | 77 | 39 | 2 | 46 | 122 | 31 | 102 | 2.87e-14 | 63.5 |
| Msa0373960 | MtrunA17_Chr2g0330071 | 42.105 | 76 | 27 | 2 | 47 | 122 | 70 | 128 | 8.73e-14 | 63.2 |
| Msa0373960 | MtrunA17_Chr4g0063871 | 38.824 | 85 | 38 | 2 | 39 | 122 | 43 | 114 | 3.18e-13 | 61.2 |
| Msa0373960 | MtrunA17_Chr2g0333071 | 34.146 | 123 | 69 | 5 | 1 | 122 | 2 | 113 | 1.19e-12 | 59.7 |
| Msa0373960 | MtrunA17_Chr5g0400761 | 37.662 | 77 | 43 | 2 | 46 | 122 | 60 | 131 | 3.68e-12 | 58.9 |
| Msa0373960 | MtrunA17_Chr8g0342581 | 37.975 | 79 | 28 | 2 | 44 | 122 | 72 | 129 | 3.92e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0373960 | AT5G10310.1 | 47.573 | 103 | 44 | 3 | 26 | 122 | 24 | 122 | 2.15e-25 | 93.2 |
| Msa0373960 | AT5G10310.2 | 48.837 | 86 | 35 | 2 | 42 | 122 | 47 | 128 | 4.66e-23 | 87.4 |
Find 46 sgRNAs with CRISPR-Local
Find 154 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATAACCTCATCTTGTTAA+TGG | 0.240049 | 3_1:-73105648 | Msa0373960:CDS |
| AAGAAACAATGAAATTTCTA+AGG | 0.259527 | 3_1:+73105011 | None:intergenic |
| TGTAGTGGAGGCTGAGATAG+AGG | 0.383685 | 3_1:+73105975 | None:intergenic |
| ATCCGTTATTATTATTACCT+TGG | 0.417680 | 3_1:+73105090 | None:intergenic |
| GGTTGATAGAAGCAAGAAAC+TGG | 0.429204 | 3_1:+73105593 | None:intergenic |
| GGCTGAACATGTTCATGGCT+TGG | 0.438174 | 3_1:+73105161 | None:intergenic |
| GACCTGGCTGAACATGTTCA+TGG | 0.454543 | 3_1:+73105156 | None:intergenic |
| CTCCAAGGTAATAATAATAA+CGG | 0.464837 | 3_1:-73105092 | Msa0373960:CDS |
| TGTGTAGGGTTTATTATTTG+AGG | 0.467998 | 3_1:-73105264 | Msa0373960:intron |
| AAGAAACTGGACACAGTAGA+AGG | 0.491502 | 3_1:+73105606 | None:intergenic |
| CAATGGCATTTCCAACTCAA+TGG | 0.495776 | 3_1:+73105053 | None:intergenic |
| AAAGAAATTACCACCTTCCA+TGG | 0.501489 | 3_1:+73105115 | None:intergenic |
| GGAACTTGCACTGCCATGCA+TGG | 0.505648 | 3_1:+73105182 | None:intergenic |
| CACTGCCTCAACTTCATCCA+TGG | 0.509432 | 3_1:-73105132 | Msa0373960:CDS |
| CATTTGTTGTGACATGTTGG+TGG | 0.511699 | 3_1:+73105218 | None:intergenic |
| AGGGAAAAGCCTACCCTTGG+AGG | 0.512819 | 3_1:+73105557 | None:intergenic |
| ATTTCTAAGGGTTGAAGAAG+TGG | 0.515036 | 3_1:+73105024 | None:intergenic |
| GGAGAGGGATAGTGTAGTGG+AGG | 0.517083 | 3_1:+73105963 | None:intergenic |
| CAAATTACAAGCCATTGAGT+TGG | 0.518087 | 3_1:-73105064 | Msa0373960:CDS |
| GGTAACAATTGTGTAGAGAA+AGG | 0.520211 | 3_1:+73105942 | None:intergenic |
| AAAGGAGAGGGATAGTGTAG+TGG | 0.532448 | 3_1:+73105960 | None:intergenic |
| GGTGGTAATTTCTTTCTCCA+AGG | 0.542564 | 3_1:-73105107 | Msa0373960:CDS |
| CACATTCTGCAATTCCTCCA+AGG | 0.543179 | 3_1:-73105571 | Msa0373960:CDS |
| AGAAACAATGAAATTTCTAA+GGG | 0.551654 | 3_1:+73105012 | None:intergenic |
| AGATAGAGGGTGCAGAGTGT+TGG | 0.551815 | 3_1:+73105989 | None:intergenic |
| AGCCATGAACATGTTCAGCC+AGG | 0.557195 | 3_1:-73105158 | Msa0373960:CDS |
| GAAGTTGAGGCAGTGTGACC+TGG | 0.560053 | 3_1:+73105140 | None:intergenic |
| AATTGTGTAGAGAAAGGAGA+GGG | 0.568206 | 3_1:+73105948 | None:intergenic |
| ACCTTCCATGGATGAAGTTG+AGG | 0.576895 | 3_1:+73105127 | None:intergenic |
| GCCTCAACTTCATCCATGGA+AGG | 0.583985 | 3_1:-73105128 | Msa0373960:CDS |
| TTACATTTGTTGTGACATGT+TGG | 0.595097 | 3_1:+73105215 | None:intergenic |
| ATATGAATTAAATGAAGCCA+TGG | 0.598193 | 3_1:+73105667 | None:intergenic |
| GTAGTGGAGGCTGAGATAGA+GGG | 0.606138 | 3_1:+73105976 | None:intergenic |
| TGATGACCATTAACAAGATG+AGG | 0.607087 | 3_1:+73105642 | None:intergenic |
| TCAGCAGTGATTGTATACAT+TGG | 0.616295 | 3_1:-73104884 | Msa0373960:three_prime_UTR |
| TTGAGTTGGAAATGCCATTG+TGG | 0.619909 | 3_1:-73105050 | Msa0373960:CDS |
| TGAAGAAGTGGCCACCACAA+TGG | 0.621299 | 3_1:+73105036 | None:intergenic |
| TAACCAATGTCATCCATGCA+TGG | 0.626151 | 3_1:-73105195 | Msa0373960:CDS |
| ACATTCTGCAATTCCTCCAA+GGG | 0.630675 | 3_1:-73105570 | Msa0373960:intron |
| ACATGATAGATAGATATCCA+TGG | 0.649649 | 3_1:-73105684 | Msa0373960:exon |
| AGTGTTGGCAGAAAAGTGAA+AGG | 0.656008 | 3_1:+73106004 | None:intergenic |
| TCAACTTCATCCATGGAAGG+TGG | 0.660618 | 3_1:-73105125 | Msa0373960:CDS |
| CTGCCATGCATGGATGACAT+TGG | 0.662535 | 3_1:+73105192 | None:intergenic |
| CTTGGAGGAATTGCAGAATG+TGG | 0.672231 | 3_1:+73105572 | None:intergenic |
| AGTTGGAAATGCCATTGTGG+TGG | 0.687706 | 3_1:-73105047 | Msa0373960:CDS |
| CAATTGTGTAGAGAAAGGAG+AGG | 0.717317 | 3_1:+73105947 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTTGAATTCACTATAAAAT+TGG | + | chr3_1:73105497-73105516 | None:intergenic | 20.0% |
| !! | AGTTAACAAACTTAATTAAC+TGG | + | chr3_1:73105572-73105591 | None:intergenic | 20.0% |
| !! | AGAAACAATGAAATTTCTAA+GGG | + | chr3_1:73105850-73105869 | None:intergenic | 20.0% |
| !! | AAGAAACAATGAAATTTCTA+AGG | + | chr3_1:73105851-73105870 | None:intergenic | 20.0% |
| !! | GTTTGAATTCACTATAAAAT+TGG | + | chr3_1:73105497-73105516 | None:intergenic | 20.0% |
| !! | AGTTAACAAACTTAATTAAC+TGG | + | chr3_1:73105572-73105591 | None:intergenic | 20.0% |
| !! | AGAAACAATGAAATTTCTAA+GGG | + | chr3_1:73105850-73105869 | None:intergenic | 20.0% |
| !! | AAGAAACAATGAAATTTCTA+AGG | + | chr3_1:73105851-73105870 | None:intergenic | 20.0% |
| !!! | GTTTTTTTCTAGTTATGTGT+TGG | + | chr3_1:73104945-73104964 | None:intergenic | 25.0% |
| !! | AATGACAAACTTTTCATTCT+TGG | - | chr3_1:73104989-73105008 | Msa0373960:three_prime_UTR | 25.0% |
| !!! | TTATTTTGTCACATTACAAC+TGG | - | chr3_1:73105060-73105079 | Msa0373960:CDS | 25.0% |
| ! | ATATGAATTAAATGAAGCCA+TGG | + | chr3_1:73105195-73105214 | None:intergenic | 25.0% |
| ! | ATATAACCTCATCTTGTTAA+TGG | - | chr3_1:73105211-73105230 | Msa0373960:CDS | 25.0% |
| !!! | TAGTTGGTTTTAAAAGGAAT+CGG | - | chr3_1:73105372-73105391 | Msa0373960:intron | 25.0% |
| !! | ACAGTATATTACTGTGATTT+TGG | - | chr3_1:73105430-73105449 | Msa0373960:intron | 25.0% |
| ! | AATTCACTATAAAATTGGCT+CGG | + | chr3_1:73105492-73105511 | None:intergenic | 25.0% |
| ! | TATTATTTGAGGAGAAAAAC+AGG | - | chr3_1:73105606-73105625 | Msa0373960:CDS | 25.0% |
| ! | CTCCAAGGTAATAATAATAA+CGG | - | chr3_1:73105767-73105786 | Msa0373960:five_prime_UTR | 25.0% |
| ! | ATCCGTTATTATTATTACCT+TGG | + | chr3_1:73105772-73105791 | None:intergenic | 25.0% |
| !!! | GTTTTTTTCTAGTTATGTGT+TGG | + | chr3_1:73104945-73104964 | None:intergenic | 25.0% |
| !! | AATGACAAACTTTTCATTCT+TGG | - | chr3_1:73104989-73105008 | Msa0373960:three_prime_UTR | 25.0% |
| !!! | TTATTTTGTCACATTACAAC+TGG | - | chr3_1:73105060-73105079 | Msa0373960:CDS | 25.0% |
| ! | ATATGAATTAAATGAAGCCA+TGG | + | chr3_1:73105195-73105214 | None:intergenic | 25.0% |
| ! | ATATAACCTCATCTTGTTAA+TGG | - | chr3_1:73105211-73105230 | Msa0373960:CDS | 25.0% |
| !!! | TAGTTGGTTTTAAAAGGAAT+CGG | - | chr3_1:73105372-73105391 | Msa0373960:intron | 25.0% |
| !! | ACAGTATATTACTGTGATTT+TGG | - | chr3_1:73105430-73105449 | Msa0373960:intron | 25.0% |
| ! | AATTCACTATAAAATTGGCT+CGG | + | chr3_1:73105492-73105511 | None:intergenic | 25.0% |
| ! | TATTATTTGAGGAGAAAAAC+AGG | - | chr3_1:73105606-73105625 | Msa0373960:CDS | 25.0% |
| ! | CTCCAAGGTAATAATAATAA+CGG | - | chr3_1:73105767-73105786 | Msa0373960:five_prime_UTR | 25.0% |
| ! | ATCCGTTATTATTATTACCT+TGG | + | chr3_1:73105772-73105791 | None:intergenic | 25.0% |
| !!! | ATCTAGTTTTGAGCAATGAA+TGG | + | chr3_1:73104967-73104986 | None:intergenic | 30.0% |
| ACATGATAGATAGATATCCA+TGG | - | chr3_1:73105175-73105194 | Msa0373960:CDS | 30.0% | |
| TTATGTGTATGTTGCATAGT+TGG | - | chr3_1:73105356-73105375 | Msa0373960:intron | 30.0% | |
| !!! | GTTGCATAGTTGGTTTTAAA+AGG | - | chr3_1:73105366-73105385 | Msa0373960:intron | 30.0% |
| ACAGTAATATACTGTGATTC+TGG | + | chr3_1:73105425-73105444 | None:intergenic | 30.0% | |
| ! | ATCCTCATTTTGTTGAAACT+TGG | + | chr3_1:73105525-73105544 | None:intergenic | 30.0% |
| ACTTAATTAACTGGTGAGTT+TGG | + | chr3_1:73105563-73105582 | None:intergenic | 30.0% | |
| !! | AGTTTGTTAACTATGTGTGT+AGG | - | chr3_1:73105580-73105599 | Msa0373960:CDS | 30.0% |
| ! | GTTTGTTAACTATGTGTGTA+GGG | - | chr3_1:73105581-73105600 | Msa0373960:CDS | 30.0% |
| TGTGTAGGGTTTATTATTTG+AGG | - | chr3_1:73105595-73105614 | Msa0373960:CDS | 30.0% | |
| !! | TTACATTTGTTGTGACATGT+TGG | + | chr3_1:73105647-73105666 | None:intergenic | 30.0% |
| !!! | ATCTAGTTTTGAGCAATGAA+TGG | + | chr3_1:73104967-73104986 | None:intergenic | 30.0% |
| ACATGATAGATAGATATCCA+TGG | - | chr3_1:73105175-73105194 | Msa0373960:CDS | 30.0% | |
| TTATGTGTATGTTGCATAGT+TGG | - | chr3_1:73105356-73105375 | Msa0373960:intron | 30.0% | |
| !!! | GTTGCATAGTTGGTTTTAAA+AGG | - | chr3_1:73105366-73105385 | Msa0373960:intron | 30.0% |
| ACAGTAATATACTGTGATTC+TGG | + | chr3_1:73105425-73105444 | None:intergenic | 30.0% | |
| ! | ATCCTCATTTTGTTGAAACT+TGG | + | chr3_1:73105525-73105544 | None:intergenic | 30.0% |
| ACTTAATTAACTGGTGAGTT+TGG | + | chr3_1:73105563-73105582 | None:intergenic | 30.0% | |
| !! | AGTTTGTTAACTATGTGTGT+AGG | - | chr3_1:73105580-73105599 | Msa0373960:CDS | 30.0% |
| ! | GTTTGTTAACTATGTGTGTA+GGG | - | chr3_1:73105581-73105600 | Msa0373960:CDS | 30.0% |
| TGTGTAGGGTTTATTATTTG+AGG | - | chr3_1:73105595-73105614 | Msa0373960:CDS | 30.0% | |
| !! | TTACATTTGTTGTGACATGT+TGG | + | chr3_1:73105647-73105666 | None:intergenic | 30.0% |
| AATTGTGTAGAGAAAGGAGA+GGG | + | chr3_1:73104914-73104933 | None:intergenic | 35.0% | |
| GGTAACAATTGTGTAGAGAA+AGG | + | chr3_1:73104920-73104939 | None:intergenic | 35.0% | |
| !!! | TTTTCTAGTTATGTGTTGGC+AGG | + | chr3_1:73104941-73104960 | None:intergenic | 35.0% |
| TGATGACCATTAACAAGATG+AGG | + | chr3_1:73105220-73105239 | None:intergenic | 35.0% | |
| AGTGAATGAGGGAGAATAAA+GGG | + | chr3_1:73105324-73105343 | None:intergenic | 35.0% | |
| GCAACATACACATAAAGCAA+AGG | + | chr3_1:73105351-73105370 | None:intergenic | 35.0% | |
| CACCAAGTTTCAACAAAATG+AGG | - | chr3_1:73105520-73105539 | Msa0373960:intron | 35.0% | |
| TTTGAGGAGAAAAACAGGTT+AGG | - | chr3_1:73105611-73105630 | Msa0373960:CDS | 35.0% | |
| AAAGAAATTACCACCTTCCA+TGG | + | chr3_1:73105747-73105766 | None:intergenic | 35.0% | |
| CAAATTACAAGCCATTGAGT+TGG | - | chr3_1:73105795-73105814 | Msa0373960:five_prime_UTR | 35.0% | |
| ! | ATTTCTAAGGGTTGAAGAAG+TGG | + | chr3_1:73105838-73105857 | None:intergenic | 35.0% |
| TCAGCAGTGATTGTATACAT+TGG | - | chr3_1:73105975-73105994 | Msa0373960:five_prime_UTR | 35.0% | |
| AATTGTGTAGAGAAAGGAGA+GGG | + | chr3_1:73104914-73104933 | None:intergenic | 35.0% | |
| GGTAACAATTGTGTAGAGAA+AGG | + | chr3_1:73104920-73104939 | None:intergenic | 35.0% | |
| !!! | TTTTCTAGTTATGTGTTGGC+AGG | + | chr3_1:73104941-73104960 | None:intergenic | 35.0% |
| TGATGACCATTAACAAGATG+AGG | + | chr3_1:73105220-73105239 | None:intergenic | 35.0% | |
| AGTGAATGAGGGAGAATAAA+GGG | + | chr3_1:73105324-73105343 | None:intergenic | 35.0% | |
| GCAACATACACATAAAGCAA+AGG | + | chr3_1:73105351-73105370 | None:intergenic | 35.0% | |
| CACCAAGTTTCAACAAAATG+AGG | - | chr3_1:73105520-73105539 | Msa0373960:intron | 35.0% | |
| TTTGAGGAGAAAAACAGGTT+AGG | - | chr3_1:73105611-73105630 | Msa0373960:CDS | 35.0% | |
| AAAGAAATTACCACCTTCCA+TGG | + | chr3_1:73105747-73105766 | None:intergenic | 35.0% | |
| CAAATTACAAGCCATTGAGT+TGG | - | chr3_1:73105795-73105814 | Msa0373960:five_prime_UTR | 35.0% | |
| ! | ATTTCTAAGGGTTGAAGAAG+TGG | + | chr3_1:73105838-73105857 | None:intergenic | 35.0% |
| TCAGCAGTGATTGTATACAT+TGG | - | chr3_1:73105975-73105994 | Msa0373960:five_prime_UTR | 35.0% | |
| CAATTGTGTAGAGAAAGGAG+AGG | + | chr3_1:73104915-73104934 | None:intergenic | 40.0% | |
| AAGAAACTGGACACAGTAGA+AGG | + | chr3_1:73105256-73105275 | None:intergenic | 40.0% | |
| !! | GGTTGATAGAAGCAAGAAAC+TGG | + | chr3_1:73105269-73105288 | None:intergenic | 40.0% |
| ACATTCTGCAATTCCTCCAA+GGG | - | chr3_1:73105289-73105308 | Msa0373960:intron | 40.0% | |
| GAGTGAATGAGGGAGAATAA+AGG | + | chr3_1:73105325-73105344 | None:intergenic | 40.0% | |
| GCAAAGGAAAGAGTGAATGA+GGG | + | chr3_1:73105335-73105354 | None:intergenic | 40.0% | |
| AGCAAAGGAAAGAGTGAATG+AGG | + | chr3_1:73105336-73105355 | None:intergenic | 40.0% | |
| TGGTGAGTTTGGCAAAATCA+AGG | + | chr3_1:73105552-73105571 | None:intergenic | 40.0% | |
| !! | CATTTGTTGTGACATGTTGG+TGG | + | chr3_1:73105644-73105663 | None:intergenic | 40.0% |
| TAACCAATGTCATCCATGCA+TGG | - | chr3_1:73105664-73105683 | Msa0373960:CDS | 40.0% | |
| GGTGGTAATTTCTTTCTCCA+AGG | - | chr3_1:73105752-73105771 | Msa0373960:five_prime_UTR | 40.0% | |
| CAATGGCATTTCCAACTCAA+TGG | + | chr3_1:73105809-73105828 | None:intergenic | 40.0% | |
| !! | TTGAGTTGGAAATGCCATTG+TGG | - | chr3_1:73105809-73105828 | Msa0373960:five_prime_UTR | 40.0% |
| CAATTGTGTAGAGAAAGGAG+AGG | + | chr3_1:73104915-73104934 | None:intergenic | 40.0% | |
| AAGAAACTGGACACAGTAGA+AGG | + | chr3_1:73105256-73105275 | None:intergenic | 40.0% | |
| !! | GGTTGATAGAAGCAAGAAAC+TGG | + | chr3_1:73105269-73105288 | None:intergenic | 40.0% |
| ACATTCTGCAATTCCTCCAA+GGG | - | chr3_1:73105289-73105308 | Msa0373960:intron | 40.0% | |
| GAGTGAATGAGGGAGAATAA+AGG | + | chr3_1:73105325-73105344 | None:intergenic | 40.0% | |
| GCAAAGGAAAGAGTGAATGA+GGG | + | chr3_1:73105335-73105354 | None:intergenic | 40.0% | |
| AGCAAAGGAAAGAGTGAATG+AGG | + | chr3_1:73105336-73105355 | None:intergenic | 40.0% | |
| TGGTGAGTTTGGCAAAATCA+AGG | + | chr3_1:73105552-73105571 | None:intergenic | 40.0% | |
| !! | CATTTGTTGTGACATGTTGG+TGG | + | chr3_1:73105644-73105663 | None:intergenic | 40.0% |
| TAACCAATGTCATCCATGCA+TGG | - | chr3_1:73105664-73105683 | Msa0373960:CDS | 40.0% | |
| GGTGGTAATTTCTTTCTCCA+AGG | - | chr3_1:73105752-73105771 | Msa0373960:five_prime_UTR | 40.0% | |
| CAATGGCATTTCCAACTCAA+TGG | + | chr3_1:73105809-73105828 | None:intergenic | 40.0% | |
| !! | TTGAGTTGGAAATGCCATTG+TGG | - | chr3_1:73105809-73105828 | Msa0373960:five_prime_UTR | 40.0% |
| AAAGGAGAGGGATAGTGTAG+TGG | + | chr3_1:73104902-73104921 | None:intergenic | 45.0% | |
| CTTGGAGGAATTGCAGAATG+TGG | + | chr3_1:73105290-73105309 | None:intergenic | 45.0% | |
| CACATTCTGCAATTCCTCCA+AGG | - | chr3_1:73105288-73105307 | Msa0373960:intron | 45.0% | |
| TAAAGGGAAAAGCCTACCCT+TGG | + | chr3_1:73105308-73105327 | None:intergenic | 45.0% | |
| GGAATCGGTGCATGTTTGAA+TGG | - | chr3_1:73105387-73105406 | Msa0373960:intron | 45.0% | |
| TCGGTGCATGTTTGAATGGA+CGG | - | chr3_1:73105391-73105410 | Msa0373960:intron | 45.0% | |
| GCTCGGAGCTTCAACAAAAT+CGG | + | chr3_1:73105475-73105494 | None:intergenic | 45.0% | |
| ACCTTCCATGGATGAAGTTG+AGG | + | chr3_1:73105735-73105754 | None:intergenic | 45.0% | |
| TCAACTTCATCCATGGAAGG+TGG | - | chr3_1:73105734-73105753 | Msa0373960:five_prime_UTR | 45.0% | |
| AGTTGGAAATGCCATTGTGG+TGG | - | chr3_1:73105812-73105831 | Msa0373960:five_prime_UTR | 45.0% | |
| AAAGGAGAGGGATAGTGTAG+TGG | + | chr3_1:73104902-73104921 | None:intergenic | 45.0% | |
| CTTGGAGGAATTGCAGAATG+TGG | + | chr3_1:73105290-73105309 | None:intergenic | 45.0% | |
| CACATTCTGCAATTCCTCCA+AGG | - | chr3_1:73105288-73105307 | Msa0373960:intron | 45.0% | |
| TAAAGGGAAAAGCCTACCCT+TGG | + | chr3_1:73105308-73105327 | None:intergenic | 45.0% | |
| GGAATCGGTGCATGTTTGAA+TGG | - | chr3_1:73105387-73105406 | Msa0373960:intron | 45.0% | |
| TCGGTGCATGTTTGAATGGA+CGG | - | chr3_1:73105391-73105410 | Msa0373960:intron | 45.0% | |
| GCTCGGAGCTTCAACAAAAT+CGG | + | chr3_1:73105475-73105494 | None:intergenic | 45.0% | |
| ACCTTCCATGGATGAAGTTG+AGG | + | chr3_1:73105735-73105754 | None:intergenic | 45.0% | |
| TCAACTTCATCCATGGAAGG+TGG | - | chr3_1:73105734-73105753 | Msa0373960:five_prime_UTR | 45.0% | |
| AGTTGGAAATGCCATTGTGG+TGG | - | chr3_1:73105812-73105831 | Msa0373960:five_prime_UTR | 45.0% | |
| !! | TAAATAAATAAAAACAAAAA+AGG | + | chr3_1:73105932-73105951 | None:intergenic | 5.0% |
| !! | TAAATAAATAAAAACAAAAA+AGG | + | chr3_1:73105932-73105951 | None:intergenic | 5.0% |
| ! | AGATAGAGGGTGCAGAGTGT+TGG | + | chr3_1:73104873-73104892 | None:intergenic | 50.0% |
| ! | GTAGTGGAGGCTGAGATAGA+GGG | + | chr3_1:73104886-73104905 | None:intergenic | 50.0% |
| ! | TGTAGTGGAGGCTGAGATAG+AGG | + | chr3_1:73104887-73104906 | None:intergenic | 50.0% |
| TCTGCAATTCCTCCAAGGGT+AGG | - | chr3_1:73105293-73105312 | Msa0373960:intron | 50.0% | |
| CTGCCATGCATGGATGACAT+TGG | + | chr3_1:73105670-73105689 | None:intergenic | 50.0% | |
| GGCTGAACATGTTCATGGCT+TGG | + | chr3_1:73105701-73105720 | None:intergenic | 50.0% | |
| AGCCATGAACATGTTCAGCC+AGG | - | chr3_1:73105701-73105720 | Msa0373960:five_prime_UTR | 50.0% | |
| GACCTGGCTGAACATGTTCA+TGG | + | chr3_1:73105706-73105725 | None:intergenic | 50.0% | |
| CACTGCCTCAACTTCATCCA+TGG | - | chr3_1:73105727-73105746 | Msa0373960:five_prime_UTR | 50.0% | |
| GCCTCAACTTCATCCATGGA+AGG | - | chr3_1:73105731-73105750 | Msa0373960:five_prime_UTR | 50.0% | |
| TGAAGAAGTGGCCACCACAA+TGG | + | chr3_1:73105826-73105845 | None:intergenic | 50.0% | |
| ! | AGATAGAGGGTGCAGAGTGT+TGG | + | chr3_1:73104873-73104892 | None:intergenic | 50.0% |
| ! | GTAGTGGAGGCTGAGATAGA+GGG | + | chr3_1:73104886-73104905 | None:intergenic | 50.0% |
| ! | TGTAGTGGAGGCTGAGATAG+AGG | + | chr3_1:73104887-73104906 | None:intergenic | 50.0% |
| TCTGCAATTCCTCCAAGGGT+AGG | - | chr3_1:73105293-73105312 | Msa0373960:intron | 50.0% | |
| CTGCCATGCATGGATGACAT+TGG | + | chr3_1:73105670-73105689 | None:intergenic | 50.0% | |
| GGCTGAACATGTTCATGGCT+TGG | + | chr3_1:73105701-73105720 | None:intergenic | 50.0% | |
| AGCCATGAACATGTTCAGCC+AGG | - | chr3_1:73105701-73105720 | Msa0373960:five_prime_UTR | 50.0% | |
| GACCTGGCTGAACATGTTCA+TGG | + | chr3_1:73105706-73105725 | None:intergenic | 50.0% | |
| CACTGCCTCAACTTCATCCA+TGG | - | chr3_1:73105727-73105746 | Msa0373960:five_prime_UTR | 50.0% | |
| GCCTCAACTTCATCCATGGA+AGG | - | chr3_1:73105731-73105750 | Msa0373960:five_prime_UTR | 50.0% | |
| TGAAGAAGTGGCCACCACAA+TGG | + | chr3_1:73105826-73105845 | None:intergenic | 50.0% | |
| GGAGAGGGATAGTGTAGTGG+AGG | + | chr3_1:73104899-73104918 | None:intergenic | 55.0% | |
| AGGGAAAAGCCTACCCTTGG+AGG | + | chr3_1:73105305-73105324 | None:intergenic | 55.0% | |
| GGAACTTGCACTGCCATGCA+TGG | + | chr3_1:73105680-73105699 | None:intergenic | 55.0% | |
| GAAGTTGAGGCAGTGTGACC+TGG | + | chr3_1:73105722-73105741 | None:intergenic | 55.0% | |
| GGAGAGGGATAGTGTAGTGG+AGG | + | chr3_1:73104899-73104918 | None:intergenic | 55.0% | |
| AGGGAAAAGCCTACCCTTGG+AGG | + | chr3_1:73105305-73105324 | None:intergenic | 55.0% | |
| GGAACTTGCACTGCCATGCA+TGG | + | chr3_1:73105680-73105699 | None:intergenic | 55.0% | |
| GAAGTTGAGGCAGTGTGACC+TGG | + | chr3_1:73105722-73105741 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 73104857 | 73106024 | 73104857 | ID=Msa0373960;Name=Msa0373960 |
| chr3_1 | mRNA | 73104857 | 73106024 | 73104857 | ID=Msa0373960-mRNA-1;Parent=Msa0373960;Name=Msa0373960-mRNA-1;_AED=0.00;_eAED=0.00;_QI=337|1|1|1|1|1|2|171|122 |
| chr3_1 | exon | 73104857 | 73105279 | 73104857 | ID=Msa0373960-mRNA-1:exon:16622;Parent=Msa0373960-mRNA-1 |
| chr3_1 | exon | 73105571 | 73106024 | 73105571 | ID=Msa0373960-mRNA-1:exon:16621;Parent=Msa0373960-mRNA-1 |
| chr3_1 | five_prime_UTR | 73105688 | 73106024 | 73105688 | ID=Msa0373960-mRNA-1:five_prime_utr;Parent=Msa0373960-mRNA-1 |
| chr3_1 | CDS | 73105571 | 73105687 | 73105571 | ID=Msa0373960-mRNA-1:cds;Parent=Msa0373960-mRNA-1 |
| chr3_1 | CDS | 73105028 | 73105279 | 73105028 | ID=Msa0373960-mRNA-1:cds;Parent=Msa0373960-mRNA-1 |
| chr3_1 | three_prime_UTR | 73104857 | 73105027 | 73104857 | ID=Msa0373960-mRNA-1:three_prime_utr;Parent=Msa0373960-mRNA-1 |
| Gene Sequence |
| Protein sequence |
