AlfalfaGEDB | Msa1329480

M. sativa cultivar zhongmu-4 / Msa1329480

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0009628 GO:0036377 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0050896 GO:0051704 GO:0080167
PFAM: PF12697.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1329480	sp\|Q9SZU7\|KAI2_ARATH	78.810	269	57	0	1	269	1	269	4.73e-165	461

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1329480	G7JCS9	100.000	270	0	0	1	270	1	270	0.0	558

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1154320	Msa1329480	0.804863	1.761241e-49	-8.615850e-47
Msa1193940	Msa1329480	0.807202	5.670562e-50	-8.615850e-47
Msa1204780	Msa1329480	0.886700	2.721188e-72	-8.615850e-47
Msa1204790	Msa1329480	0.880457	5.402828e-70	-8.615850e-47
Msa1329390	Msa1329480	0.982149	2.348460e-154	-8.615850e-47
Msa1329430	Msa1329480	0.975976	5.963695e-141	-8.615850e-47
Msa1329480	Msa1329590	0.979057	3.855472e-147	-8.615850e-47
Msa1329480	Msa1339980	0.844475	7.388335e-59	-8.615850e-47
Msa1329480	Msa1362290	0.922433	9.891167e-89	-8.615850e-47
Msa1329480	Msa1408390	0.800785	1.224444e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1329480	MtrunA17_Chr5g0401521	80.224	268	53	0	1	268	1	268	4.52e-170	472
Msa1329480	MtrunA17_Chr4g0053741	100.000	219	0	0	52	270	1	219	6.38e-164	453
Msa1329480	MtrunA17_Chr1g0152551	57.430	249	104	2	6	253	8	255	8.00e-108	313
Msa1329480	MtrunA17_Chr3g0093821	40.408	245	144	2	9	252	13	256	2.55e-69	215
Msa1329480	MtrunA17_Chr6g0485021	40.157	254	149	3	9	260	22	274	1.71e-66	208

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1329480	AT4G37470.1	78.810	269	57	0	1	269	1	269	4.81e-166	461
Msa1329480	AT3G03990.1	57.661	248	104	1	6	253	8	254	2.97e-108	314
Msa1329480	AT3G24420.1	43.548	248	137	3	7	252	13	259	3.13e-76	233

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Find 87 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGAAAGAGCATGAAAG+AGG	0.542605	8_4:+48121391	Msa1329480:three_prime_UTR
AAACTATGGATGATAGCAAC+CGG	0.435693	8_4:-48119458	None:intergenic
AAATGAAAGAGCATGAAAGA+GGG	0.566913	8_4:+48121392	Msa1329480:three_prime_UTR
AAATTGTTTGTAACACGCTT+AGG	0.357437	8_4:-48121073	None:intergenic
AACACGCTTAGGGCTATGTC+TGG	0.562881	8_4:-48121062	None:intergenic
AACGACAGGGACTACTTTGG+AGG	0.459003	8_4:+48120891	Msa1329480:CDS
AACTATGGATGATAGCAACC+GGG	0.648957	8_4:-48119457	None:intergenic
AATGGCAGTTGGAGGGGACA+TGG	0.527300	8_4:+48120992	Msa1329480:CDS
AATTACAAATCATGGTGTTC+TGG	0.329664	8_4:+48120960	Msa1329480:CDS
AATTGTTTGTAACACGCTTA+GGG	0.419608	8_4:-48121072	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGGACCTAATTAAGAAACTA+TGG	-	chr8_4:48119475-48119494	None:intergenic	30.0%
TGTCATACTTTATGACAACA+TGG	+	chr8_4:48119785-48119804	Msa1329480:CDS	30.0%
GTCATACTTTATGACAACAT+GGG	+	chr8_4:48119786-48119805	Msa1329480:CDS	30.0%
ATCATAAGCATAACCTTCTA+AGG	-	chr8_4:48119859-48119878	None:intergenic	30.0%
TGGCATCAAATTACAAATCA+TGG	+	chr8_4:48120952-48120971	Msa1329480:CDS	30.0%
AATTACAAATCATGGTGTTC+TGG	+	chr8_4:48120960-48120979	Msa1329480:CDS	30.0%
AATTGTTTGTAACACGCTTA+GGG	-	chr8_4:48121075-48121094	None:intergenic	30.0%
AAATTGTTTGTAACACGCTT+AGG	-	chr8_4:48121076-48121095	None:intergenic	30.0%
CATGAGACAAATATTATGCA+TGG	+	chr8_4:48121106-48121125	Msa1329480:CDS	30.0%
TACTTTGTATAATGTGGCAT+GGG	-	chr8_4:48121139-48121158	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	48119458	48121624	48119458	ID=Msa1329480;Name=Msa1329480
chr8_4	mRNA	48119458	48121624	48119458	ID=Msa1329480-mRNA-1;Parent=Msa1329480;Name=Msa1329480-mRNA-1;_AED=0.00;_eAED=0.00;_QI=193\|1\|1\|1\|1\|1\|2\|296\|270
chr8_4	exon	48119458	48120018	48119458	ID=Msa1329480-mRNA-1:exon:13781;Parent=Msa1329480-mRNA-1
chr8_4	exon	48120884	48121624	48120884	ID=Msa1329480-mRNA-1:exon:13782;Parent=Msa1329480-mRNA-1
chr8_4	five_prime_UTR	48119458	48119650	48119458	ID=Msa1329480-mRNA-1:five_prime_utr;Parent=Msa1329480-mRNA-1
chr8_4	CDS	48119651	48120018	48119651	ID=Msa1329480-mRNA-1:cds;Parent=Msa1329480-mRNA-1
chr8_4	CDS	48120884	48121328	48120884	ID=Msa1329480-mRNA-1:cds;Parent=Msa1329480-mRNA-1
chr8_4	three_prime_UTR	48121329	48121624	48121329	ID=Msa1329480-mRNA-1:three_prime_utr;Parent=Msa1329480-mRNA-1

Gene Sequence

>Msa1329480

ATGGGGATAGTAGAAGAAGCTCACAACGTGAAAGTTTTGGGTTCAGGGTCACGGTTCATAGTTCTAGCTCACGGGTTTGGCACAGATCAATCTGTATGGAAACACCTCGTACCTCATCTCTTAGATGAGTTTCGTGTCATACTTTATGACAACATGGGAGCTGGTACAACTAATCCAGATTACTTTGATTTTGAGCGTTATTCAACCTTAGAAGGTTATGCTTATGATTTACTTGCAATCTTAGAAGAACTTCGAGTTGATTCATGTATCTTTGTTGGTCATTCTGTTTCTGCTATGATTGGAACTGTTGCATCAATTTCTCGACCTGATCTATTCAACAAAATTATTTTGATATCAGCTTCTCCAAGGTATTTGAACGACAGGGACTACTTTGGAGGATTTGAGCAAGAAGATTTGGATCAACTATTCGATGCTATGGCATCAAATTACAAATCATGGTGTTCTGGATTTGCTCCAATGGCAGTTGGAGGGGACATGGAGTCAGTAGCAGTACAAGAATTCAGTCGAACTTTGTTCAACATGAGACCAGACATAGCCCTAAGCGTGTTACAAACAATTTTTAAAAGTGACATGAGACAAATATTATGCATGGTCACTGTCCCATGCCACATTATACAAAGTATGAAGGATCTAGCGGTTCCAGTCGTGGTCGCGGAATATCTCCACCAACATGTTGGTAGCGAGTCCATCGTGGAGGTTATGTCGACAGAGGGTCATTTGCCGCAATTAAGCTCGCCGGACGTTGTTATTCCGGTGATACTCAAACACATTCGTCATGATATTGCGGCTTGA

Protein sequence

>Msa1329480

MGIVEEAHNVKVLGSGSRFIVLAHGFGTDQSVWKHLVPHLLDEFRVILYDNMGAGTTNPDYFDFERYSTLEGYAYDLLAILEELRVDSCIFVGHSVSAMIGTVASISRPDLFNKIILISASPRYLNDRDYFGGFEQEDLDQLFDAMASNYKSWCSGFAPMAVGGDMESVAVQEFSRTLFNMRPDIALSVLQTIFKSDMRQILCMVTVPCHIIQSMKDLAVPVVVAEYLHQHVGSESIVEVMSTEGHLPQLSSPDVVIPVILKHIRHDIAA*