AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1329480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1329480 MtrunA17_Chr5g0401521 80.224 268 53 0 1 268 1 268 4.52e-170 472
Msa1329480 MtrunA17_Chr4g0053741 100.000 219 0 0 52 270 1 219 6.38e-164 453
Msa1329480 MtrunA17_Chr1g0152551 57.430 249 104 2 6 253 8 255 8.00e-108 313
Msa1329480 MtrunA17_Chr3g0093821 40.408 245 144 2 9 252 13 256 2.55e-69 215
Msa1329480 MtrunA17_Chr6g0485021 40.157 254 149 3 9 260 22 274 1.71e-66 208
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1329480 AT4G37470.1 78.810 269 57 0 1 269 1 269 4.81e-166 461
Msa1329480 AT3G03990.1 57.661 248 104 1 6 253 8 254 2.97e-108 314
Msa1329480 AT3G24420.1 43.548 248 137 3 7 252 13 259 3.13e-76 233

Find 87 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGACCACGTCGTCATATTT+TGG 0.182565 8_4:-48119492 None:intergenic
TCATCCATAGTTTCTTAATT+AGG 0.267045 8_4:+48119468 Msa1329480:five_prime_UTR
AGCTCACAACGTGAAAGTTT+TGG 0.271854 8_4:+48119668 Msa1329480:CDS
TTGGATTAATCAGTAACTTT+AGG 0.290550 8_4:-48119620 None:intergenic
TGGACCTAATTAAGAAACTA+TGG 0.290707 8_4:-48119472 None:intergenic
AGGATTTGAGCAAGAAGATT+TGG 0.309415 8_4:+48120911 Msa1329480:CDS
CTCGCCGGACGTTGTTATTC+CGG 0.318614 8_4:+48121268 Msa1329480:CDS
GTTGCATGATTGTATTATTT+AGG 0.322130 8_4:+48121350 Msa1329480:three_prime_UTR
CGCTCAAAATCAAAGTAATC+TGG 0.326618 8_4:-48119825 None:intergenic
CATTCTGTTTCTGCTATGAT+TGG 0.327046 8_4:+48119930 Msa1329480:CDS
GAGCGTTATTCAACCTTAGA+AGG 0.329474 8_4:+48119843 Msa1329480:CDS
AATTACAAATCATGGTGTTC+TGG 0.329664 8_4:+48120960 Msa1329480:CDS
GTTGATTCATGTATCTTTGT+TGG 0.344178 8_4:+48119906 Msa1329480:CDS
GACGACGTGGTCTAAAACTT+TGG 0.346914 8_4:+48119501 Msa1329480:five_prime_UTR
AAATTGTTTGTAACACGCTT+AGG 0.357437 8_4:-48121073 None:intergenic
ATCATAAGCATAACCTTCTA+AGG 0.361019 8_4:-48119856 None:intergenic
GTTAAGGGGACTTGGGAGGG+TGG 0.362712 8_4:-48119555 None:intergenic
TAACAACAATACATAATTGA+TGG 0.378492 8_4:-48121478 None:intergenic
ATAGTTCTAGCTCACGGGTT+TGG 0.396140 8_4:+48119708 Msa1329480:CDS
TTGGTTAAGGGGACTTGGGA+GGG 0.411587 8_4:-48119558 None:intergenic
AATTGTTTGTAACACGCTTA+GGG 0.419608 8_4:-48121072 None:intergenic
AGATATTCCGCGACCACGAC+TGG 0.422541 8_4:-48121176 None:intergenic
TAGATGCTCATAATTATTGA+TGG 0.435669 8_4:-48121540 None:intergenic
AAACTATGGATGATAGCAAC+CGG 0.435693 8_4:-48119458 None:intergenic
TGGCATCAAATTACAAATCA+TGG 0.445032 8_4:+48120952 Msa1329480:CDS
AACGACAGGGACTACTTTGG+AGG 0.459003 8_4:+48120891 Msa1329480:CDS
GGATTTGCTCCAATGGCAGT+TGG 0.462285 8_4:+48120981 Msa1329480:CDS
ATGTCCCCTCCAACTGCCAT+TGG 0.469708 8_4:-48120990 None:intergenic
GCCTCCATCCCCACATTACA+AGG 0.471192 8_4:-48121600 None:intergenic
GGATCAACTATTCGATGCTA+TGG 0.479394 8_4:+48120932 Msa1329480:CDS
GTCCGTTTCGTAGATGACTA+AGG 0.483932 8_4:+48121430 Msa1329480:three_prime_UTR
CGTAGATGACTAAGGGAGAT+GGG 0.485855 8_4:+48121438 Msa1329480:three_prime_UTR
TTGAACGACAGGGACTACTT+TGG 0.492694 8_4:+48120888 Msa1329480:CDS
GGGGAAGGTATTTGAACGAC+AGG 0.494683 8_4:+48120877 Msa1329480:intron
TGCTCCAATGGCAGTTGGAG+GGG 0.510556 8_4:+48120986 Msa1329480:CDS
CCTTCATACTTTGTATAATG+TGG 0.518604 8_4:-48121142 None:intergenic
TACTTTGTATAATGTGGCAT+GGG 0.519899 8_4:-48121136 None:intergenic
TTTGGTTAAGGGGACTTGGG+AGG 0.525703 8_4:-48119559 None:intergenic
TCGTAGATGACTAAGGGAGA+TGG 0.526663 8_4:+48121437 Msa1329480:three_prime_UTR
AATGGCAGTTGGAGGGGACA+TGG 0.527300 8_4:+48120992 Msa1329480:CDS
TCCCTTAGTCATCTACGAAA+CGG 0.530875 8_4:-48121432 None:intergenic
GTGTTCTGGATTTGCTCCAA+TGG 0.532527 8_4:+48120974 Msa1329480:CDS
GGTTCATAGTTCTAGCTCAC+GGG 0.534969 8_4:+48119703 Msa1329480:CDS
TTGGCACAGATCAATCTGTA+TGG 0.535156 8_4:+48119727 Msa1329480:CDS
AGCGGTTCCAGTCGTGGTCG+CGG 0.535585 8_4:+48121169 Msa1329480:CDS
ATCACCGGAATAACAACGTC+CGG 0.537857 8_4:-48121272 None:intergenic
CCACATTATACAAAGTATGA+AGG 0.538729 8_4:+48121142 Msa1329480:CDS
TTTGCTCCAATGGCAGTTGG+AGG 0.538790 8_4:+48120984 Msa1329480:CDS
ATACTTTGTATAATGTGGCA+TGG 0.539342 8_4:-48121137 None:intergenic
AAAATGAAAGAGCATGAAAG+AGG 0.542605 8_4:+48121391 Msa1329480:three_prime_UTR
CTTTATGACAACATGGGAGC+TGG 0.543266 8_4:+48119792 Msa1329480:CDS
TTATCCTTGTAATGTGGGGA+TGG 0.544383 8_4:+48121596 Msa1329480:three_prime_UTR
TTGCTCCAATGGCAGTTGGA+GGG 0.545271 8_4:+48120985 Msa1329480:CDS
CGAATGTGTTTGAGTATCAC+CGG 0.546328 8_4:-48121287 None:intergenic
ATGTTAATGTGTGGTAGGTG+TGG 0.549229 8_4:+48121328 Msa1329480:exon
GTGTTGTTATCCTTGTAATG+TGG 0.555830 8_4:+48121590 Msa1329480:three_prime_UTR
CGGTTCATAGTTCTAGCTCA+CGG 0.558590 8_4:+48119702 Msa1329480:CDS
TGTTGTTATCCTTGTAATGT+GGG 0.561522 8_4:+48121591 Msa1329480:three_prime_UTR
AACACGCTTAGGGCTATGTC+TGG 0.562881 8_4:-48121062 None:intergenic
AAATGAAAGAGCATGAAAGA+GGG 0.566913 8_4:+48121392 Msa1329480:three_prime_UTR
TCCTTGTAATGTGGGGATGG+AGG 0.573982 8_4:+48121599 Msa1329480:three_prime_UTR
ACTCGCTACCAACATGTTGG+TGG 0.576429 8_4:-48121199 None:intergenic
ATGACTAAGGGAGATGGGGT+TGG 0.576791 8_4:+48121443 Msa1329480:three_prime_UTR
GGATCTAGCGGTTCCAGTCG+TGG 0.576985 8_4:+48121163 Msa1329480:CDS
TCCGTTTCGTAGATGACTAA+GGG 0.581710 8_4:+48121431 Msa1329480:three_prime_UTR
ACACATTCGTCATGATATTG+CGG 0.602861 8_4:+48121301 Msa1329480:CDS
GAATATCTCCACCAACATGT+TGG 0.605480 8_4:+48121191 Msa1329480:CDS
GCTTGATGTTAATGTGTGGT+AGG 0.607429 8_4:+48121323 Msa1329480:exon
TGGACTCGCTACCAACATGT+TGG 0.613943 8_4:-48121202 None:intergenic
CATGAGACAAATATTATGCA+TGG 0.615267 8_4:+48121106 Msa1329480:CDS
TTTGCCGCAATTAAGCTCGC+CGG 0.620386 8_4:+48121253 Msa1329480:CDS
TGTCATACTTTATGACAACA+TGG 0.621368 8_4:+48119785 Msa1329480:CDS
GTGGAGGTTATGTCGACAGA+GGG 0.648761 8_4:+48121227 Msa1329480:CDS
AACTATGGATGATAGCAACC+GGG 0.648957 8_4:-48119457 None:intergenic
CGAAACTCATCTAAGAGATG+AGG 0.649391 8_4:-48119762 None:intergenic
GTTGTTATCCTTGTAATGTG+GGG 0.654182 8_4:+48121592 Msa1329480:three_prime_UTR
ACAAAGTATGAAGGATCTAG+CGG 0.659829 8_4:+48121151 Msa1329480:CDS
GGGAAGGTATTTGAACGACA+GGG 0.664911 8_4:+48120878 Msa1329480:intron
GTAGATGACTAAGGGAGATG+GGG 0.666306 8_4:+48121439 Msa1329480:three_prime_UTR
GTCATACTTTATGACAACAT+GGG 0.668133 8_4:+48119786 Msa1329480:CDS
CGTGGAGGTTATGTCGACAG+AGG 0.671230 8_4:+48121226 Msa1329480:CDS
CATCTAAGAGATGAGGTACG+AGG 0.688542 8_4:-48119755 None:intergenic
TGCGGCTTGATGTTAATGTG+TGG 0.709045 8_4:+48121319 Msa1329480:exon
TGTTGGTAGCGAGTCCATCG+TGG 0.709388 8_4:+48121208 Msa1329480:CDS
TGGTAGCGAGTCCATCGTGG+AGG 0.724029 8_4:+48121211 Msa1329480:CDS
TGTCGACATAACCTCCACGA+TGG 0.724152 8_4:-48121222 None:intergenic
AGGTCCAAAATATGACGACG+TGG 0.734949 8_4:+48119488 Msa1329480:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTAGTCCAATATTTA+TGG - chr8_4:48120203-48120222 None:intergenic 15.0%
!!! TAAATATCATATATTTTGAG+TGG + chr8_4:48120431-48120450 Msa1329480:intron 15.0%
!!! TTTTGATCATTATATTATGT+AGG + chr8_4:48120492-48120511 Msa1329480:intron 15.0%
!! TAATGTATCAATAGTTAATA+AGG + chr8_4:48120617-48120636 Msa1329480:intron 15.0%
!! ATTATTAATGATGTAAATGA+TGG + chr8_4:48120846-48120865 Msa1329480:intron 15.0%
!! TTATTAATGATGTAAATGAT+GGG + chr8_4:48120847-48120866 Msa1329480:intron 15.0%
!! AAAAATTAGTCCAATATTTA+TGG - chr8_4:48120203-48120222 None:intergenic 15.0%
!!! TAAATATCATATATTTTGAG+TGG + chr8_4:48120431-48120450 Msa1329480:intron 15.0%
!!! TTTTGATCATTATATTATGT+AGG + chr8_4:48120492-48120511 Msa1329480:intron 15.0%
!! TAATGTATCAATAGTTAATA+AGG + chr8_4:48120617-48120636 Msa1329480:intron 15.0%
!! ATTATTAATGATGTAAATGA+TGG + chr8_4:48120846-48120865 Msa1329480:intron 15.0%
!! TTATTAATGATGTAAATGAT+GGG + chr8_4:48120847-48120866 Msa1329480:intron 15.0%
!!! TTTTTCTTTGTTTTGGTTAA+GGG - chr8_4:48119573-48119592 None:intergenic 20.0%
!!! GTATTTGTTTTTCTTTGTTT+TGG - chr8_4:48119580-48119599 None:intergenic 20.0%
!! ATTAATCCAACACAAAAAAT+GGG + chr8_4:48119633-48119652 Msa1329480:exon 20.0%
!!! ATAATTTTGTTGAATAGATC+AGG - chr8_4:48119978-48119997 None:intergenic 20.0%
!! GATTAATCATTGTACAATAA+TGG + chr8_4:48120095-48120114 Msa1329480:intron 20.0%
!!! TTTTTTGTCTCCATAAATAT+TGG + chr8_4:48120190-48120209 Msa1329480:intron 20.0%
!! GATAAAAATTATCTCTTGAA+AGG - chr8_4:48120347-48120366 None:intergenic 20.0%
!! ATTATATTATGTAGGCATAT+TGG + chr8_4:48120500-48120519 Msa1329480:intron 20.0%
!!! TTTTTTCTTTCTTTCATCTT+TGG + chr8_4:48120679-48120698 Msa1329480:intron 20.0%
!! TATTAATGATGTAAATGATG+GGG + chr8_4:48120848-48120867 Msa1329480:intron 20.0%
!! TAACAACAATACATAATTGA+TGG - chr8_4:48121481-48121500 None:intergenic 20.0%
!!! GTTATCCTTTTTTTTACATA+AGG + chr8_4:48121568-48121587 Msa1329480:three_prime_UTR 20.0%
!!! TTTTTCTTTGTTTTGGTTAA+GGG - chr8_4:48119573-48119592 None:intergenic 20.0%
!!! GTATTTGTTTTTCTTTGTTT+TGG - chr8_4:48119580-48119599 None:intergenic 20.0%
!! ATTAATCCAACACAAAAAAT+GGG + chr8_4:48119633-48119652 Msa1329480:exon 20.0%
!!! ATAATTTTGTTGAATAGATC+AGG - chr8_4:48119978-48119997 None:intergenic 20.0%
!! GATTAATCATTGTACAATAA+TGG + chr8_4:48120095-48120114 Msa1329480:intron 20.0%
!!! TTTTTTGTCTCCATAAATAT+TGG + chr8_4:48120190-48120209 Msa1329480:intron 20.0%
!! GATAAAAATTATCTCTTGAA+AGG - chr8_4:48120347-48120366 None:intergenic 20.0%
!! ATTATATTATGTAGGCATAT+TGG + chr8_4:48120500-48120519 Msa1329480:intron 20.0%
!!! TTTTTTCTTTCTTTCATCTT+TGG + chr8_4:48120679-48120698 Msa1329480:intron 20.0%
!! TATTAATGATGTAAATGATG+GGG + chr8_4:48120848-48120867 Msa1329480:intron 20.0%
!! TAACAACAATACATAATTGA+TGG - chr8_4:48121481-48121500 None:intergenic 20.0%
!!! GTTATCCTTTTTTTTACATA+AGG + chr8_4:48121568-48121587 Msa1329480:three_prime_UTR 20.0%
! TCATCCATAGTTTCTTAATT+AGG + chr8_4:48119468-48119487 Msa1329480:five_prime_UTR 25.0%
!!! TTTTCTTTGTTTTGGTTAAG+GGG - chr8_4:48119572-48119591 None:intergenic 25.0%
!!! GTTTTTCTTTGTTTTGGTTA+AGG - chr8_4:48119574-48119593 None:intergenic 25.0%
! TTGGATTAATCAGTAACTTT+AGG - chr8_4:48119623-48119642 None:intergenic 25.0%
! GATTAATCCAACACAAAAAA+TGG + chr8_4:48119632-48119651 Msa1329480:exon 25.0%
! TTAATCCAACACAAAAAATG+GGG + chr8_4:48119634-48119653 Msa1329480:exon 25.0%
!!! AATTTAATTTGACCATACCT+TGG - chr8_4:48120017-48120036 None:intergenic 25.0%
!! TCAACAAAGTCTTATATTTG+AGG - chr8_4:48120293-48120312 None:intergenic 25.0%
! TGATCAAAATAAAACTGCAT+AGG - chr8_4:48120481-48120500 None:intergenic 25.0%
! ATTAATGATGTAAATGATGG+GGG + chr8_4:48120849-48120868 Msa1329480:intron 25.0%
! GTTGCATGATTGTATTATTT+AGG + chr8_4:48121350-48121369 Msa1329480:three_prime_UTR 25.0%
!!! TATGCTAGTGTTTTTAAGTT+TGG + chr8_4:48121505-48121524 Msa1329480:three_prime_UTR 25.0%
! TAGATGCTCATAATTATTGA+TGG - chr8_4:48121543-48121562 None:intergenic 25.0%
! CAACACCTTATGTAAAAAAA+AGG - chr8_4:48121576-48121595 None:intergenic 25.0%
! TCATCCATAGTTTCTTAATT+AGG + chr8_4:48119468-48119487 Msa1329480:five_prime_UTR 25.0%
!!! TTTTCTTTGTTTTGGTTAAG+GGG - chr8_4:48119572-48119591 None:intergenic 25.0%
!!! GTTTTTCTTTGTTTTGGTTA+AGG - chr8_4:48119574-48119593 None:intergenic 25.0%
! TTGGATTAATCAGTAACTTT+AGG - chr8_4:48119623-48119642 None:intergenic 25.0%
! GATTAATCCAACACAAAAAA+TGG + chr8_4:48119632-48119651 Msa1329480:exon 25.0%
! TTAATCCAACACAAAAAATG+GGG + chr8_4:48119634-48119653 Msa1329480:exon 25.0%
!!! AATTTAATTTGACCATACCT+TGG - chr8_4:48120017-48120036 None:intergenic 25.0%
!! TCAACAAAGTCTTATATTTG+AGG - chr8_4:48120293-48120312 None:intergenic 25.0%
! TGATCAAAATAAAACTGCAT+AGG - chr8_4:48120481-48120500 None:intergenic 25.0%
! ATTAATGATGTAAATGATGG+GGG + chr8_4:48120849-48120868 Msa1329480:intron 25.0%
! GTTGCATGATTGTATTATTT+AGG + chr8_4:48121350-48121369 Msa1329480:three_prime_UTR 25.0%
!!! TATGCTAGTGTTTTTAAGTT+TGG + chr8_4:48121505-48121524 Msa1329480:three_prime_UTR 25.0%
! TAGATGCTCATAATTATTGA+TGG - chr8_4:48121543-48121562 None:intergenic 25.0%
! CAACACCTTATGTAAAAAAA+AGG - chr8_4:48121576-48121595 None:intergenic 25.0%
TGGACCTAATTAAGAAACTA+TGG - chr8_4:48119475-48119494 None:intergenic 30.0%
TGTCATACTTTATGACAACA+TGG + chr8_4:48119785-48119804 Msa1329480:CDS 30.0%
GTCATACTTTATGACAACAT+GGG + chr8_4:48119786-48119805 Msa1329480:CDS 30.0%
ATCATAAGCATAACCTTCTA+AGG - chr8_4:48119859-48119878 None:intergenic 30.0%
! GTTGATTCATGTATCTTTGT+TGG + chr8_4:48119906-48119925 Msa1329480:CDS 30.0%
! AAGGATGCACCTTTAAAAAA+AGG + chr8_4:48120636-48120655 Msa1329480:intron 30.0%
!!! CTAGTGCATCCTTTTTTTAA+AGG - chr8_4:48120648-48120667 None:intergenic 30.0%
! TTAATGATGTAAATGATGGG+GGG + chr8_4:48120850-48120869 Msa1329480:intron 30.0%
TGGCATCAAATTACAAATCA+TGG + chr8_4:48120952-48120971 Msa1329480:CDS 30.0%
AATTACAAATCATGGTGTTC+TGG + chr8_4:48120960-48120979 Msa1329480:CDS 30.0%
AATTGTTTGTAACACGCTTA+GGG - chr8_4:48121075-48121094 None:intergenic 30.0%
AAATTGTTTGTAACACGCTT+AGG - chr8_4:48121076-48121095 None:intergenic 30.0%
CATGAGACAAATATTATGCA+TGG + chr8_4:48121106-48121125 Msa1329480:CDS 30.0%
TACTTTGTATAATGTGGCAT+GGG - chr8_4:48121139-48121158 None:intergenic 30.0%
ATACTTTGTATAATGTGGCA+TGG - chr8_4:48121140-48121159 None:intergenic 30.0%
CCTTCATACTTTGTATAATG+TGG - chr8_4:48121145-48121164 None:intergenic 30.0%
CCACATTATACAAAGTATGA+AGG + chr8_4:48121142-48121161 Msa1329480:CDS 30.0%
! AAAATGAAAGAGCATGAAAG+AGG + chr8_4:48121391-48121410 Msa1329480:three_prime_UTR 30.0%
! AAATGAAAGAGCATGAAAGA+GGG + chr8_4:48121392-48121411 Msa1329480:three_prime_UTR 30.0%
!!! CTAGTGTTTTTAAGTTTGGT+AGG + chr8_4:48121509-48121528 Msa1329480:three_prime_UTR 30.0%
TGTTGTTATCCTTGTAATGT+GGG + chr8_4:48121591-48121610 Msa1329480:three_prime_UTR 30.0%
TGGACCTAATTAAGAAACTA+TGG - chr8_4:48119475-48119494 None:intergenic 30.0%
TGTCATACTTTATGACAACA+TGG + chr8_4:48119785-48119804 Msa1329480:CDS 30.0%
GTCATACTTTATGACAACAT+GGG + chr8_4:48119786-48119805 Msa1329480:CDS 30.0%
ATCATAAGCATAACCTTCTA+AGG - chr8_4:48119859-48119878 None:intergenic 30.0%
! GTTGATTCATGTATCTTTGT+TGG + chr8_4:48119906-48119925 Msa1329480:CDS 30.0%
! AAGGATGCACCTTTAAAAAA+AGG + chr8_4:48120636-48120655 Msa1329480:intron 30.0%
!!! CTAGTGCATCCTTTTTTTAA+AGG - chr8_4:48120648-48120667 None:intergenic 30.0%
! TTAATGATGTAAATGATGGG+GGG + chr8_4:48120850-48120869 Msa1329480:intron 30.0%
TGGCATCAAATTACAAATCA+TGG + chr8_4:48120952-48120971 Msa1329480:CDS 30.0%
AATTACAAATCATGGTGTTC+TGG + chr8_4:48120960-48120979 Msa1329480:CDS 30.0%
AATTGTTTGTAACACGCTTA+GGG - chr8_4:48121075-48121094 None:intergenic 30.0%
AAATTGTTTGTAACACGCTT+AGG - chr8_4:48121076-48121095 None:intergenic 30.0%
CATGAGACAAATATTATGCA+TGG + chr8_4:48121106-48121125 Msa1329480:CDS 30.0%
TACTTTGTATAATGTGGCAT+GGG - chr8_4:48121139-48121158 None:intergenic 30.0%
ATACTTTGTATAATGTGGCA+TGG - chr8_4:48121140-48121159 None:intergenic 30.0%
CCTTCATACTTTGTATAATG+TGG - chr8_4:48121145-48121164 None:intergenic 30.0%
CCACATTATACAAAGTATGA+AGG + chr8_4:48121142-48121161 Msa1329480:CDS 30.0%
! AAAATGAAAGAGCATGAAAG+AGG + chr8_4:48121391-48121410 Msa1329480:three_prime_UTR 30.0%
! AAATGAAAGAGCATGAAAGA+GGG + chr8_4:48121392-48121411 Msa1329480:three_prime_UTR 30.0%
!!! CTAGTGTTTTTAAGTTTGGT+AGG + chr8_4:48121509-48121528 Msa1329480:three_prime_UTR 30.0%
TGTTGTTATCCTTGTAATGT+GGG + chr8_4:48121591-48121610 Msa1329480:three_prime_UTR 30.0%
AAACTATGGATGATAGCAAC+CGG - chr8_4:48119461-48119480 None:intergenic 35.0%
!!! ACTATCCCCATTTTTTGTGT+TGG - chr8_4:48119642-48119661 None:intergenic 35.0%
CGCTCAAAATCAAAGTAATC+TGG - chr8_4:48119828-48119847 None:intergenic 35.0%
!! CATTCTGTTTCTGCTATGAT+TGG + chr8_4:48119930-48119949 Msa1329480:CDS 35.0%
! TTTTGATATCAGCTTCTCCA+AGG + chr8_4:48119997-48120016 Msa1329480:CDS 35.0%
AGGATTTGAGCAAGAAGATT+TGG + chr8_4:48120911-48120930 Msa1329480:CDS 35.0%
ACAAAGTATGAAGGATCTAG+CGG + chr8_4:48121151-48121170 Msa1329480:CDS 35.0%
ACACATTCGTCATGATATTG+CGG + chr8_4:48121301-48121320 Msa1329480:CDS 35.0%
!!! GTGTTTTTAAGTTTGGTAGG+TGG + chr8_4:48121512-48121531 Msa1329480:three_prime_UTR 35.0%
! GTGTTGTTATCCTTGTAATG+TGG + chr8_4:48121590-48121609 Msa1329480:three_prime_UTR 35.0%
GTTGTTATCCTTGTAATGTG+GGG + chr8_4:48121592-48121611 Msa1329480:three_prime_UTR 35.0%
AAACTATGGATGATAGCAAC+CGG - chr8_4:48119461-48119480 None:intergenic 35.0%
!!! ACTATCCCCATTTTTTGTGT+TGG - chr8_4:48119642-48119661 None:intergenic 35.0%
CGCTCAAAATCAAAGTAATC+TGG - chr8_4:48119828-48119847 None:intergenic 35.0%
!! CATTCTGTTTCTGCTATGAT+TGG + chr8_4:48119930-48119949 Msa1329480:CDS 35.0%
! TTTTGATATCAGCTTCTCCA+AGG + chr8_4:48119997-48120016 Msa1329480:CDS 35.0%
AGGATTTGAGCAAGAAGATT+TGG + chr8_4:48120911-48120930 Msa1329480:CDS 35.0%
ACAAAGTATGAAGGATCTAG+CGG + chr8_4:48121151-48121170 Msa1329480:CDS 35.0%
ACACATTCGTCATGATATTG+CGG + chr8_4:48121301-48121320 Msa1329480:CDS 35.0%
!!! GTGTTTTTAAGTTTGGTAGG+TGG + chr8_4:48121512-48121531 Msa1329480:three_prime_UTR 35.0%
! GTGTTGTTATCCTTGTAATG+TGG + chr8_4:48121590-48121609 Msa1329480:three_prime_UTR 35.0%
GTTGTTATCCTTGTAATGTG+GGG + chr8_4:48121592-48121611 Msa1329480:three_prime_UTR 35.0%
! TAGACCACGTCGTCATATTT+TGG - chr8_4:48119495-48119514 None:intergenic 40.0%
!!! TGTTTTGGTTAAGGGGACTT+GGG - chr8_4:48119565-48119584 None:intergenic 40.0%
!!! TTGTTTTGGTTAAGGGGACT+TGG - chr8_4:48119566-48119585 None:intergenic 40.0%
! AGCTCACAACGTGAAAGTTT+TGG + chr8_4:48119668-48119687 Msa1329480:CDS 40.0%
! GCTCACAACGTGAAAGTTTT+GGG + chr8_4:48119669-48119688 Msa1329480:CDS 40.0%
!!! AACGTGAAAGTTTTGGGTTC+AGG + chr8_4:48119675-48119694 Msa1329480:CDS 40.0%
!!! ACGTGAAAGTTTTGGGTTCA+GGG + chr8_4:48119676-48119695 Msa1329480:CDS 40.0%
TTGGCACAGATCAATCTGTA+TGG + chr8_4:48119727-48119746 Msa1329480:CDS 40.0%
CGAAACTCATCTAAGAGATG+AGG - chr8_4:48119765-48119784 None:intergenic 40.0%
GAGCGTTATTCAACCTTAGA+AGG + chr8_4:48119843-48119862 Msa1329480:CDS 40.0%
ATATCAGCTTCTCCAAGGTA+TGG + chr8_4:48120002-48120021 Msa1329480:intron 40.0%
AGTGAGTGTGTGTGTGTATA+TGG + chr8_4:48120758-48120777 Msa1329480:intron 40.0%
! ATGTAAATGATGGGGGGATT+TGG + chr8_4:48120856-48120875 Msa1329480:intron 40.0%
! TGTAAATGATGGGGGGATTT+GGG + chr8_4:48120857-48120876 Msa1329480:intron 40.0%
GGATCAACTATTCGATGCTA+TGG + chr8_4:48120932-48120951 Msa1329480:CDS 40.0%
GAATATCTCCACCAACATGT+TGG + chr8_4:48121191-48121210 Msa1329480:CDS 40.0%
CGAATGTGTTTGAGTATCAC+CGG - chr8_4:48121290-48121309 None:intergenic 40.0%
GCTTGATGTTAATGTGTGGT+AGG + chr8_4:48121323-48121342 Msa1329480:exon 40.0%
ATGTTAATGTGTGGTAGGTG+TGG + chr8_4:48121328-48121347 Msa1329480:exon 40.0%
TCCGTTTCGTAGATGACTAA+GGG + chr8_4:48121431-48121450 Msa1329480:three_prime_UTR 40.0%
TCCCTTAGTCATCTACGAAA+CGG - chr8_4:48121435-48121454 None:intergenic 40.0%
TTATCCTTGTAATGTGGGGA+TGG + chr8_4:48121596-48121615 Msa1329480:three_prime_UTR 40.0%
! TAGACCACGTCGTCATATTT+TGG - chr8_4:48119495-48119514 None:intergenic 40.0%
!!! TGTTTTGGTTAAGGGGACTT+GGG - chr8_4:48119565-48119584 None:intergenic 40.0%
!!! TTGTTTTGGTTAAGGGGACT+TGG - chr8_4:48119566-48119585 None:intergenic 40.0%
! AGCTCACAACGTGAAAGTTT+TGG + chr8_4:48119668-48119687 Msa1329480:CDS 40.0%
! GCTCACAACGTGAAAGTTTT+GGG + chr8_4:48119669-48119688 Msa1329480:CDS 40.0%
!!! AACGTGAAAGTTTTGGGTTC+AGG + chr8_4:48119675-48119694 Msa1329480:CDS 40.0%
!!! ACGTGAAAGTTTTGGGTTCA+GGG + chr8_4:48119676-48119695 Msa1329480:CDS 40.0%
TTGGCACAGATCAATCTGTA+TGG + chr8_4:48119727-48119746 Msa1329480:CDS 40.0%
CGAAACTCATCTAAGAGATG+AGG - chr8_4:48119765-48119784 None:intergenic 40.0%
GAGCGTTATTCAACCTTAGA+AGG + chr8_4:48119843-48119862 Msa1329480:CDS 40.0%
ATATCAGCTTCTCCAAGGTA+TGG + chr8_4:48120002-48120021 Msa1329480:intron 40.0%
AGTGAGTGTGTGTGTGTATA+TGG + chr8_4:48120758-48120777 Msa1329480:intron 40.0%
! ATGTAAATGATGGGGGGATT+TGG + chr8_4:48120856-48120875 Msa1329480:intron 40.0%
! TGTAAATGATGGGGGGATTT+GGG + chr8_4:48120857-48120876 Msa1329480:intron 40.0%
GGATCAACTATTCGATGCTA+TGG + chr8_4:48120932-48120951 Msa1329480:CDS 40.0%
GAATATCTCCACCAACATGT+TGG + chr8_4:48121191-48121210 Msa1329480:CDS 40.0%
CGAATGTGTTTGAGTATCAC+CGG - chr8_4:48121290-48121309 None:intergenic 40.0%
GCTTGATGTTAATGTGTGGT+AGG + chr8_4:48121323-48121342 Msa1329480:exon 40.0%
ATGTTAATGTGTGGTAGGTG+TGG + chr8_4:48121328-48121347 Msa1329480:exon 40.0%
TCCGTTTCGTAGATGACTAA+GGG + chr8_4:48121431-48121450 Msa1329480:three_prime_UTR 40.0%
TCCCTTAGTCATCTACGAAA+CGG - chr8_4:48121435-48121454 None:intergenic 40.0%
TTATCCTTGTAATGTGGGGA+TGG + chr8_4:48121596-48121615 Msa1329480:three_prime_UTR 40.0%
AGGTCCAAAATATGACGACG+TGG + chr8_4:48119488-48119507 Msa1329480:five_prime_UTR 45.0%
! GACGACGTGGTCTAAAACTT+TGG + chr8_4:48119501-48119520 Msa1329480:five_prime_UTR 45.0%
!!! AAGTTTTGGGTTCAGGGTCA+CGG + chr8_4:48119682-48119701 Msa1329480:CDS 45.0%
!! CGGTTCATAGTTCTAGCTCA+CGG + chr8_4:48119702-48119721 Msa1329480:CDS 45.0%
!! GGTTCATAGTTCTAGCTCAC+GGG + chr8_4:48119703-48119722 Msa1329480:CDS 45.0%
!! ATAGTTCTAGCTCACGGGTT+TGG + chr8_4:48119708-48119727 Msa1329480:CDS 45.0%
CATCTAAGAGATGAGGTACG+AGG - chr8_4:48119758-48119777 None:intergenic 45.0%
CTTTATGACAACATGGGAGC+TGG + chr8_4:48119792-48119811 Msa1329480:CDS 45.0%
! GTAAATGATGGGGGGATTTG+GGG + chr8_4:48120858-48120877 Msa1329480:intron 45.0%
!! GGGAAGGTATTTGAACGACA+GGG + chr8_4:48120878-48120897 Msa1329480:intron 45.0%
TTGAACGACAGGGACTACTT+TGG + chr8_4:48120888-48120907 Msa1329480:CDS 45.0%
! GTGTTCTGGATTTGCTCCAA+TGG + chr8_4:48120974-48120993 Msa1329480:CDS 45.0%
ATCACCGGAATAACAACGTC+CGG - chr8_4:48121275-48121294 None:intergenic 45.0%
TGCGGCTTGATGTTAATGTG+TGG + chr8_4:48121319-48121338 Msa1329480:exon 45.0%
GTCCGTTTCGTAGATGACTA+AGG + chr8_4:48121430-48121449 Msa1329480:three_prime_UTR 45.0%
TCGTAGATGACTAAGGGAGA+TGG + chr8_4:48121437-48121456 Msa1329480:three_prime_UTR 45.0%
CGTAGATGACTAAGGGAGAT+GGG + chr8_4:48121438-48121457 Msa1329480:three_prime_UTR 45.0%
GTAGATGACTAAGGGAGATG+GGG + chr8_4:48121439-48121458 Msa1329480:three_prime_UTR 45.0%
AGGTCCAAAATATGACGACG+TGG + chr8_4:48119488-48119507 Msa1329480:five_prime_UTR 45.0%
! GACGACGTGGTCTAAAACTT+TGG + chr8_4:48119501-48119520 Msa1329480:five_prime_UTR 45.0%
!!! AAGTTTTGGGTTCAGGGTCA+CGG + chr8_4:48119682-48119701 Msa1329480:CDS 45.0%
!! CGGTTCATAGTTCTAGCTCA+CGG + chr8_4:48119702-48119721 Msa1329480:CDS 45.0%
!! GGTTCATAGTTCTAGCTCAC+GGG + chr8_4:48119703-48119722 Msa1329480:CDS 45.0%
!! ATAGTTCTAGCTCACGGGTT+TGG + chr8_4:48119708-48119727 Msa1329480:CDS 45.0%
CATCTAAGAGATGAGGTACG+AGG - chr8_4:48119758-48119777 None:intergenic 45.0%
CTTTATGACAACATGGGAGC+TGG + chr8_4:48119792-48119811 Msa1329480:CDS 45.0%
! GTAAATGATGGGGGGATTTG+GGG + chr8_4:48120858-48120877 Msa1329480:intron 45.0%
!! GGGAAGGTATTTGAACGACA+GGG + chr8_4:48120878-48120897 Msa1329480:intron 45.0%
TTGAACGACAGGGACTACTT+TGG + chr8_4:48120888-48120907 Msa1329480:CDS 45.0%
! GTGTTCTGGATTTGCTCCAA+TGG + chr8_4:48120974-48120993 Msa1329480:CDS 45.0%
ATCACCGGAATAACAACGTC+CGG - chr8_4:48121275-48121294 None:intergenic 45.0%
TGCGGCTTGATGTTAATGTG+TGG + chr8_4:48121319-48121338 Msa1329480:exon 45.0%
GTCCGTTTCGTAGATGACTA+AGG + chr8_4:48121430-48121449 Msa1329480:three_prime_UTR 45.0%
TCGTAGATGACTAAGGGAGA+TGG + chr8_4:48121437-48121456 Msa1329480:three_prime_UTR 45.0%
CGTAGATGACTAAGGGAGAT+GGG + chr8_4:48121438-48121457 Msa1329480:three_prime_UTR 45.0%
GTAGATGACTAAGGGAGATG+GGG + chr8_4:48121439-48121458 Msa1329480:three_prime_UTR 45.0%
!!! TAAATTATAATATTGATTTT+AGG + chr8_4:48120228-48120247 Msa1329480:intron 5.0%
!!! AAATATTAATTTTATTCTTA+AGG + chr8_4:48120564-48120583 Msa1329480:intron 5.0%
!!! TAAATTATAATATTGATTTT+AGG + chr8_4:48120228-48120247 Msa1329480:intron 5.0%
!!! AAATATTAATTTTATTCTTA+AGG + chr8_4:48120564-48120583 Msa1329480:intron 5.0%
TTGGTTAAGGGGACTTGGGA+GGG - chr8_4:48119561-48119580 None:intergenic 50.0%
TTTGGTTAAGGGGACTTGGG+AGG - chr8_4:48119562-48119581 None:intergenic 50.0%
!! GGGGAAGGTATTTGAACGAC+AGG + chr8_4:48120877-48120896 Msa1329480:intron 50.0%
AACGACAGGGACTACTTTGG+AGG + chr8_4:48120891-48120910 Msa1329480:CDS 50.0%
! GGATTTGCTCCAATGGCAGT+TGG + chr8_4:48120981-48121000 Msa1329480:CDS 50.0%
TTTGCTCCAATGGCAGTTGG+AGG + chr8_4:48120984-48121003 Msa1329480:CDS 50.0%
TTGCTCCAATGGCAGTTGGA+GGG + chr8_4:48120985-48121004 Msa1329480:CDS 50.0%
AACACGCTTAGGGCTATGTC+TGG - chr8_4:48121065-48121084 None:intergenic 50.0%
ACTCGCTACCAACATGTTGG+TGG - chr8_4:48121202-48121221 None:intergenic 50.0%
TGGACTCGCTACCAACATGT+TGG - chr8_4:48121205-48121224 None:intergenic 50.0%
TGTCGACATAACCTCCACGA+TGG - chr8_4:48121225-48121244 None:intergenic 50.0%
GTGGAGGTTATGTCGACAGA+GGG + chr8_4:48121227-48121246 Msa1329480:CDS 50.0%
TTTGCCGCAATTAAGCTCGC+CGG + chr8_4:48121253-48121272 Msa1329480:CDS 50.0%
ATGACTAAGGGAGATGGGGT+TGG + chr8_4:48121443-48121462 Msa1329480:three_prime_UTR 50.0%
TCCTTGTAATGTGGGGATGG+AGG + chr8_4:48121599-48121618 Msa1329480:three_prime_UTR 50.0%
TTGGTTAAGGGGACTTGGGA+GGG - chr8_4:48119561-48119580 None:intergenic 50.0%
TTTGGTTAAGGGGACTTGGG+AGG - chr8_4:48119562-48119581 None:intergenic 50.0%
!! GGGGAAGGTATTTGAACGAC+AGG + chr8_4:48120877-48120896 Msa1329480:intron 50.0%
AACGACAGGGACTACTTTGG+AGG + chr8_4:48120891-48120910 Msa1329480:CDS 50.0%
! GGATTTGCTCCAATGGCAGT+TGG + chr8_4:48120981-48121000 Msa1329480:CDS 50.0%
TTTGCTCCAATGGCAGTTGG+AGG + chr8_4:48120984-48121003 Msa1329480:CDS 50.0%
TTGCTCCAATGGCAGTTGGA+GGG + chr8_4:48120985-48121004 Msa1329480:CDS 50.0%
AACACGCTTAGGGCTATGTC+TGG - chr8_4:48121065-48121084 None:intergenic 50.0%
ACTCGCTACCAACATGTTGG+TGG - chr8_4:48121202-48121221 None:intergenic 50.0%
TGGACTCGCTACCAACATGT+TGG - chr8_4:48121205-48121224 None:intergenic 50.0%
TGTCGACATAACCTCCACGA+TGG - chr8_4:48121225-48121244 None:intergenic 50.0%
GTGGAGGTTATGTCGACAGA+GGG + chr8_4:48121227-48121246 Msa1329480:CDS 50.0%
TTTGCCGCAATTAAGCTCGC+CGG + chr8_4:48121253-48121272 Msa1329480:CDS 50.0%
ATGACTAAGGGAGATGGGGT+TGG + chr8_4:48121443-48121462 Msa1329480:three_prime_UTR 50.0%
TCCTTGTAATGTGGGGATGG+AGG + chr8_4:48121599-48121618 Msa1329480:three_prime_UTR 50.0%
! ATGATGGGGGGATTTGGGGA+AGG + chr8_4:48120862-48120881 Msa1329480:intron 55.0%
TGCTCCAATGGCAGTTGGAG+GGG + chr8_4:48120986-48121005 Msa1329480:CDS 55.0%
ATGTCCCCTCCAACTGCCAT+TGG - chr8_4:48120993-48121012 None:intergenic 55.0%
AATGGCAGTTGGAGGGGACA+TGG + chr8_4:48120992-48121011 Msa1329480:CDS 55.0%
AGATATTCCGCGACCACGAC+TGG - chr8_4:48121179-48121198 None:intergenic 55.0%
!! TGTTGGTAGCGAGTCCATCG+TGG + chr8_4:48121208-48121227 Msa1329480:CDS 55.0%
CGTGGAGGTTATGTCGACAG+AGG + chr8_4:48121226-48121245 Msa1329480:CDS 55.0%
ACGTCCGGCGAGCTTAATTG+CGG - chr8_4:48121260-48121279 None:intergenic 55.0%
! CTCGCCGGACGTTGTTATTC+CGG + chr8_4:48121268-48121287 Msa1329480:CDS 55.0%
GCCTCCATCCCCACATTACA+AGG - chr8_4:48121603-48121622 None:intergenic 55.0%
! ATGATGGGGGGATTTGGGGA+AGG + chr8_4:48120862-48120881 Msa1329480:intron 55.0%
TGCTCCAATGGCAGTTGGAG+GGG + chr8_4:48120986-48121005 Msa1329480:CDS 55.0%
ATGTCCCCTCCAACTGCCAT+TGG - chr8_4:48120993-48121012 None:intergenic 55.0%
AATGGCAGTTGGAGGGGACA+TGG + chr8_4:48120992-48121011 Msa1329480:CDS 55.0%
AGATATTCCGCGACCACGAC+TGG - chr8_4:48121179-48121198 None:intergenic 55.0%
!! TGTTGGTAGCGAGTCCATCG+TGG + chr8_4:48121208-48121227 Msa1329480:CDS 55.0%
CGTGGAGGTTATGTCGACAG+AGG + chr8_4:48121226-48121245 Msa1329480:CDS 55.0%
ACGTCCGGCGAGCTTAATTG+CGG - chr8_4:48121260-48121279 None:intergenic 55.0%
! CTCGCCGGACGTTGTTATTC+CGG + chr8_4:48121268-48121287 Msa1329480:CDS 55.0%
GCCTCCATCCCCACATTACA+AGG - chr8_4:48121603-48121622 None:intergenic 55.0%
GTTAAGGGGACTTGGGAGGG+TGG - chr8_4:48119558-48119577 None:intergenic 60.0%
GGATCTAGCGGTTCCAGTCG+TGG + chr8_4:48121163-48121182 Msa1329480:CDS 60.0%
! TGGTAGCGAGTCCATCGTGG+AGG + chr8_4:48121211-48121230 Msa1329480:CDS 60.0%
GTTAAGGGGACTTGGGAGGG+TGG - chr8_4:48119558-48119577 None:intergenic 60.0%
GGATCTAGCGGTTCCAGTCG+TGG + chr8_4:48121163-48121182 Msa1329480:CDS 60.0%
! TGGTAGCGAGTCCATCGTGG+AGG + chr8_4:48121211-48121230 Msa1329480:CDS 60.0%
AGCGGTTCCAGTCGTGGTCG+CGG + chr8_4:48121169-48121188 Msa1329480:CDS 65.0%
AGCGGTTCCAGTCGTGGTCG+CGG + chr8_4:48121169-48121188 Msa1329480:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 48119458 48121624 48119458 ID=Msa1329480;Name=Msa1329480
chr8_4 mRNA 48119458 48121624 48119458 ID=Msa1329480-mRNA-1;Parent=Msa1329480;Name=Msa1329480-mRNA-1;_AED=0.00;_eAED=0.00;_QI=193|1|1|1|1|1|2|296|270
chr8_4 exon 48119458 48120018 48119458 ID=Msa1329480-mRNA-1:exon:13781;Parent=Msa1329480-mRNA-1
chr8_4 exon 48120884 48121624 48120884 ID=Msa1329480-mRNA-1:exon:13782;Parent=Msa1329480-mRNA-1
chr8_4 five_prime_UTR 48119458 48119650 48119458 ID=Msa1329480-mRNA-1:five_prime_utr;Parent=Msa1329480-mRNA-1
chr8_4 CDS 48119651 48120018 48119651 ID=Msa1329480-mRNA-1:cds;Parent=Msa1329480-mRNA-1
chr8_4 CDS 48120884 48121328 48120884 ID=Msa1329480-mRNA-1:cds;Parent=Msa1329480-mRNA-1
chr8_4 three_prime_UTR 48121329 48121624 48121329 ID=Msa1329480-mRNA-1:three_prime_utr;Parent=Msa1329480-mRNA-1
Gene Sequence

>Msa1329480

ATGGGGATAGTAGAAGAAGCTCACAACGTGAAAGTTTTGGGTTCAGGGTCACGGTTCATAGTTCTAGCTCACGGGTTTGGCACAGATCAATCTGTATGGAAACACCTCGTACCTCATCTCTTAGATGAGTTTCGTGTCATACTTTATGACAACATGGGAGCTGGTACAACTAATCCAGATTACTTTGATTTTGAGCGTTATTCAACCTTAGAAGGTTATGCTTATGATTTACTTGCAATCTTAGAAGAACTTCGAGTTGATTCATGTATCTTTGTTGGTCATTCTGTTTCTGCTATGATTGGAACTGTTGCATCAATTTCTCGACCTGATCTATTCAACAAAATTATTTTGATATCAGCTTCTCCAAGGTATTTGAACGACAGGGACTACTTTGGAGGATTTGAGCAAGAAGATTTGGATCAACTATTCGATGCTATGGCATCAAATTACAAATCATGGTGTTCTGGATTTGCTCCAATGGCAGTTGGAGGGGACATGGAGTCAGTAGCAGTACAAGAATTCAGTCGAACTTTGTTCAACATGAGACCAGACATAGCCCTAAGCGTGTTACAAACAATTTTTAAAAGTGACATGAGACAAATATTATGCATGGTCACTGTCCCATGCCACATTATACAAAGTATGAAGGATCTAGCGGTTCCAGTCGTGGTCGCGGAATATCTCCACCAACATGTTGGTAGCGAGTCCATCGTGGAGGTTATGTCGACAGAGGGTCATTTGCCGCAATTAAGCTCGCCGGACGTTGTTATTCCGGTGATACTCAAACACATTCGTCATGATATTGCGGCTTGA
Protein sequence

>Msa1329480

MGIVEEAHNVKVLGSGSRFIVLAHGFGTDQSVWKHLVPHLLDEFRVILYDNMGAGTTNPDYFDFERYSTLEGYAYDLLAILEELRVDSCIFVGHSVSAMIGTVASISRPDLFNKIILISASPRYLNDRDYFGGFEQEDLDQLFDAMASNYKSWCSGFAPMAVGGDMESVAVQEFSRTLFNMRPDIALSVLQTIFKSDMRQILCMVTVPCHIIQSMKDLAVPVVVAEYLHQHVGSESIVEVMSTEGHLPQLSSPDVVIPVILKHIRHDIAA*