AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1204790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1204790 MtrunA17_Chr5g0401521 79.851 268 54 0 1 268 1 268 3.92e-169 469
Msa1204790 MtrunA17_Chr4g0053741 99.543 219 1 0 52 270 1 219 5.24e-163 451
Msa1204790 MtrunA17_Chr1g0152551 57.430 249 104 2 6 253 8 255 3.21e-107 311
Msa1204790 MtrunA17_Chr3g0093821 40.408 245 144 2 9 252 13 256 6.28e-69 214
Msa1204790 MtrunA17_Chr6g0485021 40.157 254 149 3 9 260 22 274 3.04e-66 207
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1204790 AT4G37470.1 78.810 269 57 0 1 269 1 269 9.39e-166 460
Msa1204790 AT3G03990.1 57.661 248 104 1 6 253 8 254 1.07e-107 313
Msa1204790 AT3G24420.1 43.548 248 137 3 7 252 13 259 5.10e-76 233

Find 88 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTATCATCCATAGTTTCTT+AGG 0.219885 8_1:-60628816 Msa1204790:five_prime_UTR
TTGGATTAATCAGTAACTTT+AGG 0.290550 8_1:+60628666 None:intergenic
AGGATTTGAGCAAGAAGATT+TGG 0.309415 8_1:-60627175 Msa1204790:CDS
CTCGCCGGACGTTGTTATTC+CGG 0.318614 8_1:-60626818 Msa1204790:CDS
GTTGCATGATTGTATTATTT+AGG 0.322130 8_1:-60626736 Msa1204790:three_prime_UTR
CGCTCAAAATCAAAGTAATC+TGG 0.326618 8_1:+60628461 None:intergenic
CATTCTGTTTCTGCTATGAT+TGG 0.327046 8_1:-60628356 Msa1204790:CDS
GAGCGTTATTCAACCTTAGA+AGG 0.329474 8_1:-60628443 Msa1204790:CDS
AATTACAAATCATGGTGTTC+TGG 0.329664 8_1:-60627126 Msa1204790:CDS
TATGACGACGTCTAAAACTT+TGG 0.341794 8_1:-60628786 Msa1204790:five_prime_UTR
GTTGATTCATGTATCTTTGT+TGG 0.344178 8_1:-60628380 Msa1204790:CDS
AAATTGTTTGTAACACGCTT+AGG 0.357437 8_1:+60627013 None:intergenic
ATCATAAGCATAACCTTCTA+AGG 0.361019 8_1:+60628430 None:intergenic
TAACAACAATACATAATTGA+TGG 0.378492 8_1:+60626607 None:intergenic
GTTAAGGGGACTTGGGAGGG+TGG 0.379519 8_1:+60628731 None:intergenic
TTGTATTGGTTAAGGGGACT+TGG 0.393298 8_1:+60628723 None:intergenic
TATGAAGGATCTAGCGGTTC+CGG 0.394091 8_1:-60626929 Msa1204790:CDS
ATAGTTCTAGCTCACGGGTT+TGG 0.396140 8_1:-60628578 Msa1204790:CDS
TTGTTGCAAGTGTGAAATAA+AGG 0.411733 8_1:-60626426 Msa1204790:three_prime_UTR
AGGTTTACTAGTGGATTGAT+TGG 0.418597 8_1:-60626406 Msa1204790:three_prime_UTR
AATTGTTTGTAACACGCTTA+GGG 0.419608 8_1:+60627014 None:intergenic
TTGGTTAAGGGGACTTGGGA+GGG 0.433803 8_1:+60628728 None:intergenic
AAATTTCTTTGAAAACGTTA+AGG 0.434206 8_1:-60626354 Msa1204790:three_prime_UTR
TAGATGCTCATAATTATTGA+TGG 0.435669 8_1:+60626545 None:intergenic
TGGCATCAAATTACAAATCA+TGG 0.445032 8_1:-60627134 Msa1204790:CDS
TGTATTGGTTAAGGGGACTT+GGG 0.450035 8_1:+60628724 None:intergenic
AGCGGTTCCGGTCGTGGTCG+CGG 0.451096 8_1:-60626917 Msa1204790:CDS
TTGTTATCCTTGTTGTAATG+TGG 0.458466 8_1:-60626492 Msa1204790:three_prime_UTR
AACGACAGGGACTACTTTGG+AGG 0.459003 8_1:-60627195 Msa1204790:CDS
GGATCTAGCGGTTCCGGTCG+TGG 0.461437 8_1:-60626923 Msa1204790:CDS
GGATTTGCTCCAATGGCAGT+TGG 0.462285 8_1:-60627105 Msa1204790:CDS
ATGTCCCCTCCAACTGCCAT+TGG 0.469708 8_1:+60627096 None:intergenic
GGATCAACTATTCGATGCTA+TGG 0.479394 8_1:-60627154 Msa1204790:CDS
GTCCGTTTCGTAGATGACTA+AGG 0.483932 8_1:-60626655 Msa1204790:three_prime_UTR
TGTTATCCTTGTTGTAATGT+GGG 0.484856 8_1:-60626491 Msa1204790:three_prime_UTR
CGTAGATGACTAAGGGAGAT+GGG 0.485855 8_1:-60626647 Msa1204790:three_prime_UTR
TTGAACGACAGGGACTACTT+TGG 0.492694 8_1:-60627198 Msa1204790:CDS
GGGGAAGGTATTTGAACGAC+AGG 0.494683 8_1:-60627209 Msa1204790:intron
TGCTCCAATGGCAGTTGGAG+GGG 0.510556 8_1:-60627100 Msa1204790:CDS
CCTTCATACTTTGTATAATG+TGG 0.518604 8_1:+60626944 None:intergenic
TACTTTGTATAATGTGGCAT+GGG 0.519899 8_1:+60626950 None:intergenic
TCCTTGTTGTAATGTGGGGA+TGG 0.523206 8_1:-60626486 Msa1204790:three_prime_UTR
ATTGGTTAAGGGGACTTGGG+AGG 0.525703 8_1:+60628727 None:intergenic
TCGTAGATGACTAAGGGAGA+TGG 0.526663 8_1:-60626648 Msa1204790:three_prime_UTR
AATGGCAGTTGGAGGGGACA+TGG 0.527300 8_1:-60627094 Msa1204790:CDS
AGATATTCCGCGACCACGAC+CGG 0.529415 8_1:+60626910 None:intergenic
TCCCTTAGTCATCTACGAAA+CGG 0.530875 8_1:+60626653 None:intergenic
GTGAAATAAAGGTTTACTAG+TGG 0.531441 8_1:-60626415 Msa1204790:three_prime_UTR
GTGTTCTGGATTTGCTCCAA+TGG 0.532527 8_1:-60627112 Msa1204790:CDS
GGTTCATAGTTCTAGCTCAC+GGG 0.534969 8_1:-60628583 Msa1204790:CDS
TTGGCACAGATCAATCTGTA+TGG 0.535156 8_1:-60628559 Msa1204790:CDS
ATCACCGGAATAACAACGTC+CGG 0.537857 8_1:+60626814 None:intergenic
CCACATTATACAAAGTATGA+AGG 0.538729 8_1:-60626944 Msa1204790:CDS
TTTGCTCCAATGGCAGTTGG+AGG 0.538790 8_1:-60627102 Msa1204790:CDS
ATACTTTGTATAATGTGGCA+TGG 0.539342 8_1:+60626949 None:intergenic
AAAATGAAAGAGCATGAAAG+AGG 0.542605 8_1:-60626695 Msa1204790:three_prime_UTR
CTTTATGACAACATGGGAGC+TGG 0.543266 8_1:-60628494 Msa1204790:CDS
TTGCTCCAATGGCAGTTGGA+GGG 0.545271 8_1:-60627101 Msa1204790:CDS
CGAATGTGTTTGAGTATCAC+CGG 0.546328 8_1:+60626799 None:intergenic
ATGTTAATGTGTGGTAGGTG+TGG 0.549229 8_1:-60626758 Msa1204790:three_prime_UTR
CGGTTCATAGTTCTAGCTCA+CGG 0.558590 8_1:-60628584 Msa1204790:CDS
AACACGCTTAGGGCTATGTC+TGG 0.562881 8_1:+60627024 None:intergenic
AAATGAAAGAGCATGAAAGA+GGG 0.566913 8_1:-60626694 Msa1204790:three_prime_UTR
ACTCGCTACCAACATGTTGG+TGG 0.576429 8_1:+60626887 None:intergenic
ATGACTAAGGGAGATGGGGT+TGG 0.576791 8_1:-60626642 Msa1204790:three_prime_UTR
TCCGTTTCGTAGATGACTAA+GGG 0.581710 8_1:-60626654 Msa1204790:three_prime_UTR
GTTATCCTTGTTGTAATGTG+GGG 0.587274 8_1:-60626490 Msa1204790:three_prime_UTR
ACACATTCGTCATGATATTG+CGG 0.602861 8_1:-60626785 Msa1204790:CDS
GAATATCTCCACCAACATGT+TGG 0.605480 8_1:-60626895 Msa1204790:CDS
GCTTGATGTTAATGTGTGGT+AGG 0.607429 8_1:-60626763 Msa1204790:exon
TGGACTCGCTACCAACATGT+TGG 0.613943 8_1:+60626884 None:intergenic
CATGAGACAAATATTATGCA+TGG 0.615267 8_1:-60626980 Msa1204790:CDS
TTGTTGTAATGTGGGGATGG+AGG 0.616835 8_1:-60626483 Msa1204790:three_prime_UTR
TTTGCCGCAATTAAGCTCGC+CGG 0.620386 8_1:-60626833 Msa1204790:CDS
TGTCATACTTTATGACAACA+TGG 0.621368 8_1:-60628501 Msa1204790:CDS
CGAAACTCATCTAAGAGATG+AGG 0.649391 8_1:+60628524 None:intergenic
ACAAAGTATGAAGGATCTAG+CGG 0.659829 8_1:-60626935 Msa1204790:CDS
GTGGAGGTTACGTCGACAGA+GGG 0.661981 8_1:-60626859 Msa1204790:CDS
GGGAAGGTATTTGAACGACA+GGG 0.664911 8_1:-60627208 Msa1204790:intron
GTAGATGACTAAGGGAGATG+GGG 0.666306 8_1:-60626646 Msa1204790:three_prime_UTR
GTCATACTTTATGACAACAT+GGG 0.668133 8_1:-60628500 Msa1204790:CDS
CGTGGAGGTTACGTCGACAG+AGG 0.677525 8_1:-60626860 Msa1204790:CDS
CATCTAAGAGATGAGGTACG+AGG 0.688542 8_1:+60628531 None:intergenic
TCCATCCCCACATTACAACA+AGG 0.701069 8_1:+60626485 None:intergenic
TGCGGCTTGATGTTAATGTG+TGG 0.709045 8_1:-60626767 Msa1204790:exon
TGTTGGTAGCGAGTCCATCG+TGG 0.709388 8_1:-60626878 Msa1204790:CDS
TGGTAGCGAGTCCATCGTGG+AGG 0.724029 8_1:-60626875 Msa1204790:CDS
TGTCGACGTAACCTCCACGA+TGG 0.724490 8_1:+60626864 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTAGTCCAATATTTA+TGG + chr8_1:60627071-60627090 None:intergenic 15.0%
!!! TTTTGATCATTATATTATGT+AGG - chr8_1:60627559-60627578 Msa1204790:intron 15.0%
!! TAATGTATCAATAGTTAATA+AGG - chr8_1:60627684-60627703 Msa1204790:intron 15.0%
!! ATTATTAATGATGTAAATGA+TGG - chr8_1:60627914-60627933 Msa1204790:intron 15.0%
!! TTATTAATGATGTAAATGAT+GGG - chr8_1:60627915-60627934 Msa1204790:intron 15.0%
!!! TTTTTCTTTGTATTGGTTAA+GGG + chr8_1:60626441-60626460 None:intergenic 20.0%
!!! GTATTTGTTTTTCTTTGTAT+TGG + chr8_1:60626448-60626467 None:intergenic 20.0%
!! ATTAATCCAACACAAAAAAT+GGG - chr8_1:60626501-60626520 Msa1204790:three_prime_UTR 20.0%
!!! ATAATTTTGTTGAATAGATC+AGG + chr8_1:60626846-60626865 None:intergenic 20.0%
!! GATTAATCATTGTACAATAA+TGG - chr8_1:60626963-60626982 Msa1204790:CDS 20.0%
!!! TTTTTTGTCTCCATAAATAT+TGG - chr8_1:60627058-60627077 Msa1204790:CDS 20.0%
!! GATAAAAATTATCTCTTGAA+AGG + chr8_1:60627414-60627433 None:intergenic 20.0%
!!! GAAATATCATATATTTTGAG+TGG - chr8_1:60627497-60627516 Msa1204790:intron 20.0%
!! GATCAAAATAAAAACTACAT+AGG + chr8_1:60627547-60627566 None:intergenic 20.0%
!! ATTATATTATGTAGGCATAT+TGG - chr8_1:60627567-60627586 Msa1204790:intron 20.0%
!!! TTTTTTCTTTCTTTCATCTT+TGG - chr8_1:60627747-60627766 Msa1204790:intron 20.0%
!! TATTAATGATGTAAATGATG+GGG - chr8_1:60627916-60627935 Msa1204790:intron 20.0%
!! TAACAACAATACATAATTGA+TGG + chr8_1:60628550-60628569 None:intergenic 20.0%
!!! GTTATCCTTTTTTTTACATA+AGG - chr8_1:60628637-60628656 Msa1204790:CDS 20.0%
!!! AAATTTCTTTGAAAACGTTA+AGG - chr8_1:60628800-60628819 Msa1204790:five_prime_UTR 20.0%
!!! TTTTCTTTGTATTGGTTAAG+GGG + chr8_1:60626440-60626459 None:intergenic 25.0%
!!! GTTTTTCTTTGTATTGGTTA+AGG + chr8_1:60626442-60626461 None:intergenic 25.0%
! TTGGATTAATCAGTAACTTT+AGG + chr8_1:60626491-60626510 None:intergenic 25.0%
! GATTAATCCAACACAAAAAA+TGG - chr8_1:60626500-60626519 Msa1204790:three_prime_UTR 25.0%
! TTAATCCAACACAAAAAATG+GGG - chr8_1:60626502-60626521 Msa1204790:three_prime_UTR 25.0%
!!! AATTTAATTTGACCATACCT+TGG + chr8_1:60626885-60626904 None:intergenic 25.0%
! ATAAAAGACACCTTCATAAA+AGG - chr8_1:60627254-60627273 Msa1204790:intron 25.0%
!! TCAACAAAGTCTTATATTTG+AGG + chr8_1:60627360-60627379 None:intergenic 25.0%
!!! TAGTGCATCCTTTTTTTTAA+AGG + chr8_1:60627715-60627734 None:intergenic 25.0%
! ATTAATGATGTAAATGATGG+GGG - chr8_1:60627917-60627936 Msa1204790:intron 25.0%
! GTTGCATGATTGTATTATTT+AGG - chr8_1:60628418-60628437 Msa1204790:CDS 25.0%
!!! TATGCTAGTGTTTTTAAGTT+TGG - chr8_1:60628574-60628593 Msa1204790:CDS 25.0%
! TAGATGCTCATAATTATTGA+TGG + chr8_1:60628612-60628631 None:intergenic 25.0%
! CAACACCTTATGTAAAAAAA+AGG + chr8_1:60628645-60628664 None:intergenic 25.0%
! ATTTTGGACCTAAGAAACTA+TGG + chr8_1:60626349-60626368 None:intergenic 30.0%
! TTTTAGACGTCGTCATATTT+TGG + chr8_1:60626365-60626384 None:intergenic 30.0%
TGTCATACTTTATGACAACA+TGG - chr8_1:60626653-60626672 Msa1204790:three_prime_UTR 30.0%
GTCATACTTTATGACAACAT+GGG - chr8_1:60626654-60626673 Msa1204790:three_prime_UTR 30.0%
ATCATAAGCATAACCTTCTA+AGG + chr8_1:60626727-60626746 None:intergenic 30.0%
! GTTGATTCATGTATCTTTGT+TGG - chr8_1:60626774-60626793 Msa1204790:exon 30.0%
! TTTTATCAAGTCACACCTTA+AGG - chr8_1:60627139-60627158 Msa1204790:CDS 30.0%
AAAGTGAAAATCACACCTTA+AGG + chr8_1:60627157-60627176 None:intergenic 30.0%
!! AAAGACACCAACTTACTTTT+AGG + chr8_1:60627209-60627228 None:intergenic 30.0%
! ATAGAAGACACCTTTTATGA+AGG + chr8_1:60627267-60627286 None:intergenic 30.0%
! AGGATGCACCTTTAAAAAAA+AGG - chr8_1:60627704-60627723 Msa1204790:intron 30.0%
! TTAATGATGTAAATGATGGG+GGG - chr8_1:60627918-60627937 Msa1204790:intron 30.0%
TGGCATCAAATTACAAATCA+TGG - chr8_1:60628020-60628039 Msa1204790:intron 30.0%
AATTACAAATCATGGTGTTC+TGG - chr8_1:60628028-60628047 Msa1204790:intron 30.0%
AATTGTTTGTAACACGCTTA+GGG + chr8_1:60628143-60628162 None:intergenic 30.0%
AAATTGTTTGTAACACGCTT+AGG + chr8_1:60628144-60628163 None:intergenic 30.0%
CATGAGACAAATATTATGCA+TGG - chr8_1:60628174-60628193 Msa1204790:intron 30.0%
TACTTTGTATAATGTGGCAT+GGG + chr8_1:60628207-60628226 None:intergenic 30.0%
ATACTTTGTATAATGTGGCA+TGG + chr8_1:60628208-60628227 None:intergenic 30.0%
CCTTCATACTTTGTATAATG+TGG + chr8_1:60628213-60628232 None:intergenic 30.0%
CCACATTATACAAAGTATGA+AGG - chr8_1:60628210-60628229 Msa1204790:intron 30.0%
! AAAATGAAAGAGCATGAAAG+AGG - chr8_1:60628459-60628478 Msa1204790:CDS 30.0%
! AAATGAAAGAGCATGAAAGA+GGG - chr8_1:60628460-60628479 Msa1204790:CDS 30.0%
!!! CTAGTGTTTTTAAGTTTGGT+AGG - chr8_1:60628578-60628597 Msa1204790:CDS 30.0%
! TTGTTATCCTTGTTGTAATG+TGG - chr8_1:60628662-60628681 Msa1204790:five_prime_UTR 30.0%
! TGTTATCCTTGTTGTAATGT+GGG - chr8_1:60628663-60628682 Msa1204790:five_prime_UTR 30.0%
TTGTTGCAAGTGTGAAATAA+AGG - chr8_1:60628728-60628747 Msa1204790:five_prime_UTR 30.0%
GTGAAATAAAGGTTTACTAG+TGG - chr8_1:60628739-60628758 Msa1204790:five_prime_UTR 30.0%
GCTATCATCCATAGTTTCTT+AGG - chr8_1:60626338-60626357 Msa1204790:three_prime_UTR 35.0%
! TATGACGACGTCTAAAACTT+TGG - chr8_1:60626368-60626387 Msa1204790:three_prime_UTR 35.0%
!!! ACTATCCCCATTTTTTGTGT+TGG + chr8_1:60626510-60626529 None:intergenic 35.0%
!!! AACGTGAAAGTTTTAGGATC+AGG - chr8_1:60626543-60626562 Msa1204790:three_prime_UTR 35.0%
!!! ACGTGAAAGTTTTAGGATCA+GGG - chr8_1:60626544-60626563 Msa1204790:three_prime_UTR 35.0%
CGCTCAAAATCAAAGTAATC+TGG + chr8_1:60626696-60626715 None:intergenic 35.0%
!! CATTCTGTTTCTGCTATGAT+TGG - chr8_1:60626798-60626817 Msa1204790:CDS 35.0%
! TTTTGATATCAGCTTCTCCA+AGG - chr8_1:60626865-60626884 Msa1204790:CDS 35.0%
GATAAAAAGACACCACCAAA+GGG + chr8_1:60627126-60627145 None:intergenic 35.0%
TGATAAAAAGACACCACCAA+AGG + chr8_1:60627127-60627146 None:intergenic 35.0%
ACACCTTCCTAAAAGTAAGT+TGG - chr8_1:60627199-60627218 Msa1204790:CDS 35.0%
!! ACACCAACTTACTTTTAGGA+AGG + chr8_1:60627205-60627224 None:intergenic 35.0%
AGGATTTGAGCAAGAAGATT+TGG - chr8_1:60627979-60627998 Msa1204790:intron 35.0%
ACAAAGTATGAAGGATCTAG+CGG - chr8_1:60628219-60628238 Msa1204790:intron 35.0%
ACACATTCGTCATGATATTG+CGG - chr8_1:60628369-60628388 Msa1204790:CDS 35.0%
!!! GTGTTTTTAAGTTTGGTAGG+TGG - chr8_1:60628581-60628600 Msa1204790:CDS 35.0%
! GTTATCCTTGTTGTAATGTG+GGG - chr8_1:60628664-60628683 Msa1204790:five_prime_UTR 35.0%
!! AGGTTTACTAGTGGATTGAT+TGG - chr8_1:60628748-60628767 Msa1204790:five_prime_UTR 35.0%
!! TGTATTGGTTAAGGGGACTT+GGG + chr8_1:60626433-60626452 None:intergenic 40.0%
!! TTGTATTGGTTAAGGGGACT+TGG + chr8_1:60626434-60626453 None:intergenic 40.0%
! GCTCACAACGTGAAAGTTTT+AGG - chr8_1:60626537-60626556 Msa1204790:three_prime_UTR 40.0%
!!! AAGTTTTAGGATCAGGGTCA+CGG - chr8_1:60626550-60626569 Msa1204790:three_prime_UTR 40.0%
TTGGCACAGATCAATCTGTA+TGG - chr8_1:60626595-60626614 Msa1204790:three_prime_UTR 40.0%
CGAAACTCATCTAAGAGATG+AGG + chr8_1:60626633-60626652 None:intergenic 40.0%
GAGCGTTATTCAACCTTAGA+AGG - chr8_1:60626711-60626730 Msa1204790:three_prime_UTR 40.0%
ATATCAGCTTCTCCAAGGTA+TGG - chr8_1:60626870-60626889 Msa1204790:CDS 40.0%
TTATGTTACAAGCCACCCTT+TGG - chr8_1:60627108-60627127 Msa1204790:CDS 40.0%
! ATGTAAATGATGGGGGGATT+TGG - chr8_1:60627924-60627943 Msa1204790:intron 40.0%
! TGTAAATGATGGGGGGATTT+GGG - chr8_1:60627925-60627944 Msa1204790:intron 40.0%
GGATCAACTATTCGATGCTA+TGG - chr8_1:60628000-60628019 Msa1204790:intron 40.0%
GAATATCTCCACCAACATGT+TGG - chr8_1:60628259-60628278 Msa1204790:intron 40.0%
CGAATGTGTTTGAGTATCAC+CGG + chr8_1:60628358-60628377 None:intergenic 40.0%
GCTTGATGTTAATGTGTGGT+AGG - chr8_1:60628391-60628410 Msa1204790:CDS 40.0%
ATGTTAATGTGTGGTAGGTG+TGG - chr8_1:60628396-60628415 Msa1204790:CDS 40.0%
TCCGTTTCGTAGATGACTAA+GGG - chr8_1:60628500-60628519 Msa1204790:CDS 40.0%
TCCCTTAGTCATCTACGAAA+CGG + chr8_1:60628504-60628523 None:intergenic 40.0%
!! CGGTTCATAGTTCTAGCTCA+CGG - chr8_1:60626570-60626589 Msa1204790:three_prime_UTR 45.0%
!! GGTTCATAGTTCTAGCTCAC+GGG - chr8_1:60626571-60626590 Msa1204790:three_prime_UTR 45.0%
!! ATAGTTCTAGCTCACGGGTT+TGG - chr8_1:60626576-60626595 Msa1204790:three_prime_UTR 45.0%
CATCTAAGAGATGAGGTACG+AGG + chr8_1:60626626-60626645 None:intergenic 45.0%
CTTTATGACAACATGGGAGC+TGG - chr8_1:60626660-60626679 Msa1204790:three_prime_UTR 45.0%
AAAAAGACACCACCAAAGGG+TGG + chr8_1:60627123-60627142 None:intergenic 45.0%
AGTGAGTGTGTGTGTGTGTA+TGG - chr8_1:60627826-60627845 Msa1204790:intron 45.0%
! GTAAATGATGGGGGGATTTG+GGG - chr8_1:60627926-60627945 Msa1204790:intron 45.0%
!! GGGAAGGTATTTGAACGACA+GGG - chr8_1:60627946-60627965 Msa1204790:intron 45.0%
TTGAACGACAGGGACTACTT+TGG - chr8_1:60627956-60627975 Msa1204790:intron 45.0%
! GTGTTCTGGATTTGCTCCAA+TGG - chr8_1:60628042-60628061 Msa1204790:intron 45.0%
TATGAAGGATCTAGCGGTTC+CGG - chr8_1:60628225-60628244 Msa1204790:intron 45.0%
ATCACCGGAATAACAACGTC+CGG + chr8_1:60628343-60628362 None:intergenic 45.0%
TGCGGCTTGATGTTAATGTG+TGG - chr8_1:60628387-60628406 Msa1204790:CDS 45.0%
GTCCGTTTCGTAGATGACTA+AGG - chr8_1:60628499-60628518 Msa1204790:CDS 45.0%
TCGTAGATGACTAAGGGAGA+TGG - chr8_1:60628506-60628525 Msa1204790:CDS 45.0%
CGTAGATGACTAAGGGAGAT+GGG - chr8_1:60628507-60628526 Msa1204790:CDS 45.0%
GTAGATGACTAAGGGAGATG+GGG - chr8_1:60628508-60628527 Msa1204790:CDS 45.0%
! TCCTTGTTGTAATGTGGGGA+TGG - chr8_1:60628668-60628687 Msa1204790:five_prime_UTR 45.0%
TCCATCCCCACATTACAACA+AGG + chr8_1:60628672-60628691 None:intergenic 45.0%
TTGTTGTAATGTGGGGATGG+AGG - chr8_1:60628671-60628690 Msa1204790:five_prime_UTR 45.0%
!!! AATTTTTAATGTTAAATTAT+AGG - chr8_1:60627084-60627103 Msa1204790:CDS 5.0%
!!! ATTTTTAATGTTAAATTATA+GGG - chr8_1:60627085-60627104 Msa1204790:CDS 5.0%
!! AAAATCAATATTATAATTTA+GGG + chr8_1:60627295-60627314 None:intergenic 5.0%
!! TAAAATCAATATTATAATTT+AGG + chr8_1:60627296-60627315 None:intergenic 5.0%
!!! TAAATTATAATATTGATTTT+AGG - chr8_1:60627295-60627314 Msa1204790:intron 5.0%
!!! AAATATTAATTTTATTCTTA+AGG - chr8_1:60627631-60627650 Msa1204790:intron 5.0%
TTGGTTAAGGGGACTTGGGA+GGG + chr8_1:60626429-60626448 None:intergenic 50.0%
ATTGGTTAAGGGGACTTGGG+AGG + chr8_1:60626430-60626449 None:intergenic 50.0%
TGTTACAAGCCACCCTTTGG+TGG - chr8_1:60627111-60627130 Msa1204790:CDS 50.0%
!! GGGGAAGGTATTTGAACGAC+AGG - chr8_1:60627945-60627964 Msa1204790:intron 50.0%
AACGACAGGGACTACTTTGG+AGG - chr8_1:60627959-60627978 Msa1204790:intron 50.0%
! GGATTTGCTCCAATGGCAGT+TGG - chr8_1:60628049-60628068 Msa1204790:intron 50.0%
TTTGCTCCAATGGCAGTTGG+AGG - chr8_1:60628052-60628071 Msa1204790:intron 50.0%
TTGCTCCAATGGCAGTTGGA+GGG - chr8_1:60628053-60628072 Msa1204790:intron 50.0%
AACACGCTTAGGGCTATGTC+TGG + chr8_1:60628133-60628152 None:intergenic 50.0%
ACTCGCTACCAACATGTTGG+TGG + chr8_1:60628270-60628289 None:intergenic 50.0%
TGGACTCGCTACCAACATGT+TGG + chr8_1:60628273-60628292 None:intergenic 50.0%
TTTGCCGCAATTAAGCTCGC+CGG - chr8_1:60628321-60628340 Msa1204790:CDS 50.0%
ATGACTAAGGGAGATGGGGT+TGG - chr8_1:60628512-60628531 Msa1204790:CDS 50.0%
! ATGATGGGGGGATTTGGGGA+AGG - chr8_1:60627930-60627949 Msa1204790:intron 55.0%
TGCTCCAATGGCAGTTGGAG+GGG - chr8_1:60628054-60628073 Msa1204790:intron 55.0%
ATGTCCCCTCCAACTGCCAT+TGG + chr8_1:60628061-60628080 None:intergenic 55.0%
AATGGCAGTTGGAGGGGACA+TGG - chr8_1:60628060-60628079 Msa1204790:intron 55.0%
AGATATTCCGCGACCACGAC+CGG + chr8_1:60628247-60628266 None:intergenic 55.0%
!! TGTTGGTAGCGAGTCCATCG+TGG - chr8_1:60628276-60628295 Msa1204790:intron 55.0%
TGTCGACGTAACCTCCACGA+TGG + chr8_1:60628293-60628312 None:intergenic 55.0%
GTGGAGGTTACGTCGACAGA+GGG - chr8_1:60628295-60628314 Msa1204790:CDS 55.0%
ACGTCCGGCGAGCTTAATTG+CGG + chr8_1:60628328-60628347 None:intergenic 55.0%
! CTCGCCGGACGTTGTTATTC+CGG - chr8_1:60628336-60628355 Msa1204790:CDS 55.0%
GTTAAGGGGACTTGGGAGGG+TGG + chr8_1:60626426-60626445 None:intergenic 60.0%
! TGGTAGCGAGTCCATCGTGG+AGG - chr8_1:60628279-60628298 Msa1204790:intron 60.0%
CGTGGAGGTTACGTCGACAG+AGG - chr8_1:60628294-60628313 Msa1204790:CDS 60.0%
GGATCTAGCGGTTCCGGTCG+TGG - chr8_1:60628231-60628250 Msa1204790:intron 65.0%
AGCGGTTCCGGTCGTGGTCG+CGG - chr8_1:60628237-60628256 Msa1204790:intron 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 60626335 60628841 60626335 ID=Msa1204790;Name=Msa1204790
chr8_1 mRNA 60626335 60628841 60626335 ID=Msa1204790-mRNA-1;Parent=Msa1204790;Name=Msa1204790-mRNA-1;_AED=0.00;_eAED=0.00;_QI=184|1|1|1|0|0.5|2|445|270
chr8_1 exon 60626335 60627224 60626335 ID=Msa1204790-mRNA-1:exon:19699;Parent=Msa1204790-mRNA-1
chr8_1 exon 60628290 60628841 60628290 ID=Msa1204790-mRNA-1:exon:19698;Parent=Msa1204790-mRNA-1
chr8_1 five_prime_UTR 60628658 60628841 60628658 ID=Msa1204790-mRNA-1:five_prime_utr;Parent=Msa1204790-mRNA-1
chr8_1 CDS 60628290 60628657 60628290 ID=Msa1204790-mRNA-1:cds;Parent=Msa1204790-mRNA-1
chr8_1 CDS 60626780 60627224 60626780 ID=Msa1204790-mRNA-1:cds;Parent=Msa1204790-mRNA-1
chr8_1 three_prime_UTR 60626335 60626779 60626335 ID=Msa1204790-mRNA-1:three_prime_utr;Parent=Msa1204790-mRNA-1
Gene Sequence

>Msa1204790

ATGGGGATAGTAGAAGAAGCTCACAACGTGAAAGTTTTAGGATCAGGGTCACGGTTCATAGTTCTAGCTCACGGGTTTGGCACAGATCAATCTGTATGGAAACACCTCGTACCTCATCTCTTAGATGAGTTTCGTGTCATACTTTATGACAACATGGGAGCTGGTACAACTAATCCAGATTACTTTGATTTTGAGCGTTATTCAACCTTAGAAGGTTATGCTTATGATTTACTTGCAATCTTAGAAGAACTTCGAGTTGATTCATGTATCTTTGTTGGTCATTCTGTTTCTGCTATGATTGGAACTGTTGCATCAATTTCTCGACCTGATCTATTCAACAAAATTATTTTGATATCAGCTTCTCCAAGGTATTTGAACGACAGGGACTACTTTGGAGGATTTGAGCAAGAAGATTTGGATCAACTATTCGATGCTATGGCATCAAATTACAAATCATGGTGTTCTGGATTTGCTCCAATGGCAGTTGGAGGGGACATGGAGTCAGTAGCAGTACAAGAATTCAGTCGAACTTTGTTCAACATGAGACCAGACATAGCCCTAAGCGTGTTACAAACAATTTTTAAAAGTGACATGAGACAAATATTATGCATGGTCACTGTCCCATGCCACATTATACAAAGTATGAAGGATCTAGCGGTTCCGGTCGTGGTCGCGGAATATCTCCACCAACATGTTGGTAGCGAGTCCATCGTGGAGGTTACGTCGACAGAGGGTCATTTGCCGCAATTAAGCTCGCCGGACGTTGTTATTCCGGTGATACTCAAACACATTCGTCATGATATTGCGGCTTGA
Protein sequence

>Msa1204790

MGIVEEAHNVKVLGSGSRFIVLAHGFGTDQSVWKHLVPHLLDEFRVILYDNMGAGTTNPDYFDFERYSTLEGYAYDLLAILEELRVDSCIFVGHSVSAMIGTVASISRPDLFNKIILISASPRYLNDRDYFGGFEQEDLDQLFDAMASNYKSWCSGFAPMAVGGDMESVAVQEFSRTLFNMRPDIALSVLQTIFKSDMRQILCMVTVPCHIIQSMKDLAVPVVVAEYLHQHVGSESIVEVTSTEGHLPQLSSPDVVIPVILKHIRHDIAA*