AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1204780

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1204780 MtrunA17_Chr5g0401521 79.851 268 54 0 1 268 1 268 3.92e-169 469
Msa1204780 MtrunA17_Chr4g0053741 99.543 219 1 0 52 270 1 219 5.24e-163 451
Msa1204780 MtrunA17_Chr1g0152551 57.430 249 104 2 6 253 8 255 3.21e-107 311
Msa1204780 MtrunA17_Chr3g0093821 40.408 245 144 2 9 252 13 256 6.28e-69 214
Msa1204780 MtrunA17_Chr6g0485021 40.157 254 149 3 9 260 22 274 3.04e-66 207
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1204780 AT4G37470.1 78.810 269 57 0 1 269 1 269 9.39e-166 460
Msa1204780 AT3G03990.1 57.661 248 104 1 6 253 8 254 1.07e-107 313
Msa1204780 AT3G24420.1 43.548 248 137 3 7 252 13 259 5.10e-76 233

Find 88 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTATCATCCATAGTTTCTT+AGG 0.219885 8_1:-60588407 Msa1204780:five_prime_UTR
TTGGATTAATCAGTAACTTT+AGG 0.290550 8_1:+60588257 None:intergenic
AGGATTTGAGCAAGAAGATT+TGG 0.309415 8_1:-60586764 Msa1204780:CDS
CTCGCCGGACGTTGTTATTC+CGG 0.318614 8_1:-60586407 Msa1204780:CDS
GTTGCATGATTGTATTATTT+AGG 0.322130 8_1:-60586325 Msa1204780:three_prime_UTR
CGCTCAAAATCAAAGTAATC+TGG 0.326618 8_1:+60588052 None:intergenic
CATTCTGTTTCTGCTATGAT+TGG 0.327046 8_1:-60587947 Msa1204780:CDS
GAGCGTTATTCAACCTTAGA+AGG 0.329474 8_1:-60588034 Msa1204780:CDS
AATTACAAATCATGGTGTTC+TGG 0.329664 8_1:-60586715 Msa1204780:CDS
TATGACGACGTCTAAAACTT+TGG 0.341794 8_1:-60588377 Msa1204780:five_prime_UTR
GTTGATTCATGTATCTTTGT+TGG 0.344178 8_1:-60587971 Msa1204780:CDS
AAATTGTTTGTAACACGCTT+AGG 0.357437 8_1:+60586602 None:intergenic
ATCATAAGCATAACCTTCTA+AGG 0.361019 8_1:+60588021 None:intergenic
TAACAACAATACATAATTGA+TGG 0.378492 8_1:+60586196 None:intergenic
GTTAAGGGGACTTGGGAGGG+TGG 0.379519 8_1:+60588322 None:intergenic
TTGTATTGGTTAAGGGGACT+TGG 0.393298 8_1:+60588314 None:intergenic
TATGAAGGATCTAGCGGTTC+CGG 0.394091 8_1:-60586518 Msa1204780:CDS
ATAGTTCTAGCTCACGGGTT+TGG 0.396140 8_1:-60588169 Msa1204780:CDS
TTGTTGCAAGTGTGAAATAA+AGG 0.411733 8_1:-60586015 Msa1204780:three_prime_UTR
AGGTTTACTAGTGGATTGAT+TGG 0.418597 8_1:-60585995 Msa1204780:three_prime_UTR
AATTGTTTGTAACACGCTTA+GGG 0.419608 8_1:+60586603 None:intergenic
TTGGTTAAGGGGACTTGGGA+GGG 0.433803 8_1:+60588319 None:intergenic
AAATTTCTTTGAAAACGTTA+AGG 0.434206 8_1:-60585943 Msa1204780:three_prime_UTR
TAGATGCTCATAATTATTGA+TGG 0.435669 8_1:+60586134 None:intergenic
TGGCATCAAATTACAAATCA+TGG 0.445032 8_1:-60586723 Msa1204780:CDS
TGTATTGGTTAAGGGGACTT+GGG 0.450035 8_1:+60588315 None:intergenic
AGCGGTTCCGGTCGTGGTCG+CGG 0.451096 8_1:-60586506 Msa1204780:CDS
TTGTTATCCTTGTTGTAATG+TGG 0.458466 8_1:-60586081 Msa1204780:three_prime_UTR
AACGACAGGGACTACTTTGG+AGG 0.459003 8_1:-60586784 Msa1204780:CDS
GGATCTAGCGGTTCCGGTCG+TGG 0.461437 8_1:-60586512 Msa1204780:CDS
GGATTTGCTCCAATGGCAGT+TGG 0.462285 8_1:-60586694 Msa1204780:CDS
ATGTCCCCTCCAACTGCCAT+TGG 0.469708 8_1:+60586685 None:intergenic
GGATCAACTATTCGATGCTA+TGG 0.479394 8_1:-60586743 Msa1204780:CDS
GTCCGTTTCGTAGATGACTA+AGG 0.483932 8_1:-60586244 Msa1204780:three_prime_UTR
TGTTATCCTTGTTGTAATGT+GGG 0.484856 8_1:-60586080 Msa1204780:three_prime_UTR
CGTAGATGACTAAGGGAGAT+GGG 0.485855 8_1:-60586236 Msa1204780:three_prime_UTR
TTGAACGACAGGGACTACTT+TGG 0.492694 8_1:-60586787 Msa1204780:CDS
GGGGAAGGTATTTGAACGAC+AGG 0.494683 8_1:-60586798 Msa1204780:intron
TGCTCCAATGGCAGTTGGAG+GGG 0.510556 8_1:-60586689 Msa1204780:CDS
CCTTCATACTTTGTATAATG+TGG 0.518604 8_1:+60586533 None:intergenic
TACTTTGTATAATGTGGCAT+GGG 0.519899 8_1:+60586539 None:intergenic
TCCTTGTTGTAATGTGGGGA+TGG 0.523206 8_1:-60586075 Msa1204780:three_prime_UTR
ATTGGTTAAGGGGACTTGGG+AGG 0.525703 8_1:+60588318 None:intergenic
TCGTAGATGACTAAGGGAGA+TGG 0.526663 8_1:-60586237 Msa1204780:three_prime_UTR
AATGGCAGTTGGAGGGGACA+TGG 0.527300 8_1:-60586683 Msa1204780:CDS
AGATATTCCGCGACCACGAC+CGG 0.529415 8_1:+60586499 None:intergenic
TCCCTTAGTCATCTACGAAA+CGG 0.530875 8_1:+60586242 None:intergenic
GTGAAATAAAGGTTTACTAG+TGG 0.531441 8_1:-60586004 Msa1204780:three_prime_UTR
GTGTTCTGGATTTGCTCCAA+TGG 0.532527 8_1:-60586701 Msa1204780:CDS
GGTTCATAGTTCTAGCTCAC+GGG 0.534969 8_1:-60588174 Msa1204780:CDS
TTGGCACAGATCAATCTGTA+TGG 0.535156 8_1:-60588150 Msa1204780:CDS
ATCACCGGAATAACAACGTC+CGG 0.537857 8_1:+60586403 None:intergenic
CCACATTATACAAAGTATGA+AGG 0.538729 8_1:-60586533 Msa1204780:CDS
TTTGCTCCAATGGCAGTTGG+AGG 0.538790 8_1:-60586691 Msa1204780:CDS
ATACTTTGTATAATGTGGCA+TGG 0.539342 8_1:+60586538 None:intergenic
AAAATGAAAGAGCATGAAAG+AGG 0.542605 8_1:-60586284 Msa1204780:three_prime_UTR
CTTTATGACAACATGGGAGC+TGG 0.543266 8_1:-60588085 Msa1204780:CDS
TTGCTCCAATGGCAGTTGGA+GGG 0.545271 8_1:-60586690 Msa1204780:CDS
CGAATGTGTTTGAGTATCAC+CGG 0.546328 8_1:+60586388 None:intergenic
ATGTTAATGTGTGGTAGGTG+TGG 0.549229 8_1:-60586347 Msa1204780:three_prime_UTR
CGGTTCATAGTTCTAGCTCA+CGG 0.558590 8_1:-60588175 Msa1204780:CDS
AACACGCTTAGGGCTATGTC+TGG 0.562881 8_1:+60586613 None:intergenic
AAATGAAAGAGCATGAAAGA+GGG 0.566913 8_1:-60586283 Msa1204780:three_prime_UTR
ACTCGCTACCAACATGTTGG+TGG 0.576429 8_1:+60586476 None:intergenic
ATGACTAAGGGAGATGGGGT+TGG 0.576791 8_1:-60586231 Msa1204780:three_prime_UTR
TCCGTTTCGTAGATGACTAA+GGG 0.581710 8_1:-60586243 Msa1204780:three_prime_UTR
GTTATCCTTGTTGTAATGTG+GGG 0.587274 8_1:-60586079 Msa1204780:three_prime_UTR
ACACATTCGTCATGATATTG+CGG 0.602861 8_1:-60586374 Msa1204780:CDS
GAATATCTCCACCAACATGT+TGG 0.605480 8_1:-60586484 Msa1204780:CDS
GCTTGATGTTAATGTGTGGT+AGG 0.607429 8_1:-60586352 Msa1204780:exon
TGGACTCGCTACCAACATGT+TGG 0.613943 8_1:+60586473 None:intergenic
CATGAGACAAATATTATGCA+TGG 0.615267 8_1:-60586569 Msa1204780:CDS
TTGTTGTAATGTGGGGATGG+AGG 0.616835 8_1:-60586072 Msa1204780:three_prime_UTR
TTTGCCGCAATTAAGCTCGC+CGG 0.620386 8_1:-60586422 Msa1204780:CDS
TGTCATACTTTATGACAACA+TGG 0.621368 8_1:-60588092 Msa1204780:CDS
CGAAACTCATCTAAGAGATG+AGG 0.649391 8_1:+60588115 None:intergenic
ACAAAGTATGAAGGATCTAG+CGG 0.659829 8_1:-60586524 Msa1204780:CDS
GTGGAGGTTACGTCGACAGA+GGG 0.661981 8_1:-60586448 Msa1204780:CDS
GGGAAGGTATTTGAACGACA+GGG 0.664911 8_1:-60586797 Msa1204780:intron
GTAGATGACTAAGGGAGATG+GGG 0.666306 8_1:-60586235 Msa1204780:three_prime_UTR
GTCATACTTTATGACAACAT+GGG 0.668133 8_1:-60588091 Msa1204780:CDS
CGTGGAGGTTACGTCGACAG+AGG 0.677525 8_1:-60586449 Msa1204780:CDS
CATCTAAGAGATGAGGTACG+AGG 0.688542 8_1:+60588122 None:intergenic
TCCATCCCCACATTACAACA+AGG 0.701069 8_1:+60586074 None:intergenic
TGCGGCTTGATGTTAATGTG+TGG 0.709045 8_1:-60586356 Msa1204780:exon
TGTTGGTAGCGAGTCCATCG+TGG 0.709388 8_1:-60586467 Msa1204780:CDS
TGGTAGCGAGTCCATCGTGG+AGG 0.724029 8_1:-60586464 Msa1204780:CDS
TGTCGACGTAACCTCCACGA+TGG 0.724490 8_1:+60586453 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTAGTCCAATATTTA+TGG + chr8_1:60586660-60586679 None:intergenic 15.0%
!!! TTTTGATCATTATATTATGT+AGG - chr8_1:60587148-60587167 Msa1204780:intron 15.0%
!! TAATGTATCAATAGTTAATA+AGG - chr8_1:60587273-60587292 Msa1204780:intron 15.0%
!! ATTATTAATGATGTAAATGA+TGG - chr8_1:60587505-60587524 Msa1204780:intron 15.0%
!! TTATTAATGATGTAAATGAT+GGG - chr8_1:60587506-60587525 Msa1204780:intron 15.0%
!!! TTTTTCTTTGTATTGGTTAA+GGG + chr8_1:60586030-60586049 None:intergenic 20.0%
!!! GTATTTGTTTTTCTTTGTAT+TGG + chr8_1:60586037-60586056 None:intergenic 20.0%
!! ATTAATCCAACACAAAAAAT+GGG - chr8_1:60586090-60586109 Msa1204780:three_prime_UTR 20.0%
!!! ATAATTTTGTTGAATAGATC+AGG + chr8_1:60586435-60586454 None:intergenic 20.0%
!! GATTAATCATTGTACAATAA+TGG - chr8_1:60586552-60586571 Msa1204780:CDS 20.0%
!!! TTTTTTGTCTCCATAAATAT+TGG - chr8_1:60586647-60586666 Msa1204780:CDS 20.0%
!! GATAAAAATTATCTCTTGAA+AGG + chr8_1:60587003-60587022 None:intergenic 20.0%
!!! GAAATATCATATATTTTGAG+TGG - chr8_1:60587086-60587105 Msa1204780:intron 20.0%
!! GATCAAAATAAAAACTACAT+AGG + chr8_1:60587136-60587155 None:intergenic 20.0%
!! ATTATATTATGTAGGCATAT+TGG - chr8_1:60587156-60587175 Msa1204780:intron 20.0%
!!! TTTTTTCTTTCTTTCATCTT+TGG - chr8_1:60587336-60587355 Msa1204780:intron 20.0%
!! TATTAATGATGTAAATGATG+GGG - chr8_1:60587507-60587526 Msa1204780:intron 20.0%
!! TAACAACAATACATAATTGA+TGG + chr8_1:60588141-60588160 None:intergenic 20.0%
!!! GTTATCCTTTTTTTTACATA+AGG - chr8_1:60588228-60588247 Msa1204780:CDS 20.0%
!!! AAATTTCTTTGAAAACGTTA+AGG - chr8_1:60588391-60588410 Msa1204780:five_prime_UTR 20.0%
!!! TTTTCTTTGTATTGGTTAAG+GGG + chr8_1:60586029-60586048 None:intergenic 25.0%
!!! GTTTTTCTTTGTATTGGTTA+AGG + chr8_1:60586031-60586050 None:intergenic 25.0%
! TTGGATTAATCAGTAACTTT+AGG + chr8_1:60586080-60586099 None:intergenic 25.0%
! GATTAATCCAACACAAAAAA+TGG - chr8_1:60586089-60586108 Msa1204780:three_prime_UTR 25.0%
! TTAATCCAACACAAAAAATG+GGG - chr8_1:60586091-60586110 Msa1204780:three_prime_UTR 25.0%
!!! AATTTAATTTGACCATACCT+TGG + chr8_1:60586474-60586493 None:intergenic 25.0%
! ATAAAAGACACCTTCATAAA+AGG - chr8_1:60586843-60586862 Msa1204780:intron 25.0%
!! TCAACAAAGTCTTATATTTG+AGG + chr8_1:60586949-60586968 None:intergenic 25.0%
!!! TAGTGCATCCTTTTTTTTAA+AGG + chr8_1:60587304-60587323 None:intergenic 25.0%
! ATTAATGATGTAAATGATGG+GGG - chr8_1:60587508-60587527 Msa1204780:intron 25.0%
! GTTGCATGATTGTATTATTT+AGG - chr8_1:60588009-60588028 Msa1204780:CDS 25.0%
!!! TATGCTAGTGTTTTTAAGTT+TGG - chr8_1:60588165-60588184 Msa1204780:CDS 25.0%
! TAGATGCTCATAATTATTGA+TGG + chr8_1:60588203-60588222 None:intergenic 25.0%
! CAACACCTTATGTAAAAAAA+AGG + chr8_1:60588236-60588255 None:intergenic 25.0%
! ATTTTGGACCTAAGAAACTA+TGG + chr8_1:60585938-60585957 None:intergenic 30.0%
! TTTTAGACGTCGTCATATTT+TGG + chr8_1:60585954-60585973 None:intergenic 30.0%
TGTCATACTTTATGACAACA+TGG - chr8_1:60586242-60586261 Msa1204780:three_prime_UTR 30.0%
GTCATACTTTATGACAACAT+GGG - chr8_1:60586243-60586262 Msa1204780:three_prime_UTR 30.0%
ATCATAAGCATAACCTTCTA+AGG + chr8_1:60586316-60586335 None:intergenic 30.0%
! GTTGATTCATGTATCTTTGT+TGG - chr8_1:60586363-60586382 Msa1204780:exon 30.0%
! TTTTATCAAGTCACACCTTA+AGG - chr8_1:60586728-60586747 Msa1204780:CDS 30.0%
AAAGTGAAAATCACACCTTA+AGG + chr8_1:60586746-60586765 None:intergenic 30.0%
!! AAAGACACCAACTTACTTTT+AGG + chr8_1:60586798-60586817 None:intergenic 30.0%
! ATAGAAGACACCTTTTATGA+AGG + chr8_1:60586856-60586875 None:intergenic 30.0%
! AGGATGCACCTTTAAAAAAA+AGG - chr8_1:60587293-60587312 Msa1204780:intron 30.0%
! TTAATGATGTAAATGATGGG+GGG - chr8_1:60587509-60587528 Msa1204780:intron 30.0%
TGGCATCAAATTACAAATCA+TGG - chr8_1:60587611-60587630 Msa1204780:intron 30.0%
AATTACAAATCATGGTGTTC+TGG - chr8_1:60587619-60587638 Msa1204780:intron 30.0%
AATTGTTTGTAACACGCTTA+GGG + chr8_1:60587734-60587753 None:intergenic 30.0%
AAATTGTTTGTAACACGCTT+AGG + chr8_1:60587735-60587754 None:intergenic 30.0%
CATGAGACAAATATTATGCA+TGG - chr8_1:60587765-60587784 Msa1204780:intron 30.0%
TACTTTGTATAATGTGGCAT+GGG + chr8_1:60587798-60587817 None:intergenic 30.0%
ATACTTTGTATAATGTGGCA+TGG + chr8_1:60587799-60587818 None:intergenic 30.0%
CCTTCATACTTTGTATAATG+TGG + chr8_1:60587804-60587823 None:intergenic 30.0%
CCACATTATACAAAGTATGA+AGG - chr8_1:60587801-60587820 Msa1204780:intron 30.0%
! AAAATGAAAGAGCATGAAAG+AGG - chr8_1:60588050-60588069 Msa1204780:CDS 30.0%
! AAATGAAAGAGCATGAAAGA+GGG - chr8_1:60588051-60588070 Msa1204780:CDS 30.0%
!!! CTAGTGTTTTTAAGTTTGGT+AGG - chr8_1:60588169-60588188 Msa1204780:CDS 30.0%
! TTGTTATCCTTGTTGTAATG+TGG - chr8_1:60588253-60588272 Msa1204780:five_prime_UTR 30.0%
! TGTTATCCTTGTTGTAATGT+GGG - chr8_1:60588254-60588273 Msa1204780:five_prime_UTR 30.0%
TTGTTGCAAGTGTGAAATAA+AGG - chr8_1:60588319-60588338 Msa1204780:five_prime_UTR 30.0%
GTGAAATAAAGGTTTACTAG+TGG - chr8_1:60588330-60588349 Msa1204780:five_prime_UTR 30.0%
GCTATCATCCATAGTTTCTT+AGG - chr8_1:60585927-60585946 Msa1204780:three_prime_UTR 35.0%
! TATGACGACGTCTAAAACTT+TGG - chr8_1:60585957-60585976 Msa1204780:three_prime_UTR 35.0%
!!! ACTATCCCCATTTTTTGTGT+TGG + chr8_1:60586099-60586118 None:intergenic 35.0%
!!! AACGTGAAAGTTTTAGGATC+AGG - chr8_1:60586132-60586151 Msa1204780:three_prime_UTR 35.0%
!!! ACGTGAAAGTTTTAGGATCA+GGG - chr8_1:60586133-60586152 Msa1204780:three_prime_UTR 35.0%
CGCTCAAAATCAAAGTAATC+TGG + chr8_1:60586285-60586304 None:intergenic 35.0%
!! CATTCTGTTTCTGCTATGAT+TGG - chr8_1:60586387-60586406 Msa1204780:CDS 35.0%
! TTTTGATATCAGCTTCTCCA+AGG - chr8_1:60586454-60586473 Msa1204780:CDS 35.0%
GATAAAAAGACACCACCAAA+GGG + chr8_1:60586715-60586734 None:intergenic 35.0%
TGATAAAAAGACACCACCAA+AGG + chr8_1:60586716-60586735 None:intergenic 35.0%
ACACCTTCCTAAAAGTAAGT+TGG - chr8_1:60586788-60586807 Msa1204780:CDS 35.0%
!! ACACCAACTTACTTTTAGGA+AGG + chr8_1:60586794-60586813 None:intergenic 35.0%
AGGATTTGAGCAAGAAGATT+TGG - chr8_1:60587570-60587589 Msa1204780:intron 35.0%
ACAAAGTATGAAGGATCTAG+CGG - chr8_1:60587810-60587829 Msa1204780:intron 35.0%
ACACATTCGTCATGATATTG+CGG - chr8_1:60587960-60587979 Msa1204780:CDS 35.0%
!!! GTGTTTTTAAGTTTGGTAGG+TGG - chr8_1:60588172-60588191 Msa1204780:CDS 35.0%
! GTTATCCTTGTTGTAATGTG+GGG - chr8_1:60588255-60588274 Msa1204780:five_prime_UTR 35.0%
!! AGGTTTACTAGTGGATTGAT+TGG - chr8_1:60588339-60588358 Msa1204780:five_prime_UTR 35.0%
!! TGTATTGGTTAAGGGGACTT+GGG + chr8_1:60586022-60586041 None:intergenic 40.0%
!! TTGTATTGGTTAAGGGGACT+TGG + chr8_1:60586023-60586042 None:intergenic 40.0%
! GCTCACAACGTGAAAGTTTT+AGG - chr8_1:60586126-60586145 Msa1204780:three_prime_UTR 40.0%
!!! AAGTTTTAGGATCAGGGTCA+CGG - chr8_1:60586139-60586158 Msa1204780:three_prime_UTR 40.0%
TTGGCACAGATCAATCTGTA+TGG - chr8_1:60586184-60586203 Msa1204780:three_prime_UTR 40.0%
CGAAACTCATCTAAGAGATG+AGG + chr8_1:60586222-60586241 None:intergenic 40.0%
GAGCGTTATTCAACCTTAGA+AGG - chr8_1:60586300-60586319 Msa1204780:three_prime_UTR 40.0%
ATATCAGCTTCTCCAAGGTA+TGG - chr8_1:60586459-60586478 Msa1204780:CDS 40.0%
TTATGTTACAAGCCACCCTT+TGG - chr8_1:60586697-60586716 Msa1204780:CDS 40.0%
! ATGTAAATGATGGGGGGATT+TGG - chr8_1:60587515-60587534 Msa1204780:intron 40.0%
! TGTAAATGATGGGGGGATTT+GGG - chr8_1:60587516-60587535 Msa1204780:intron 40.0%
GGATCAACTATTCGATGCTA+TGG - chr8_1:60587591-60587610 Msa1204780:intron 40.0%
GAATATCTCCACCAACATGT+TGG - chr8_1:60587850-60587869 Msa1204780:intron 40.0%
CGAATGTGTTTGAGTATCAC+CGG + chr8_1:60587949-60587968 None:intergenic 40.0%
GCTTGATGTTAATGTGTGGT+AGG - chr8_1:60587982-60588001 Msa1204780:CDS 40.0%
ATGTTAATGTGTGGTAGGTG+TGG - chr8_1:60587987-60588006 Msa1204780:CDS 40.0%
TCCGTTTCGTAGATGACTAA+GGG - chr8_1:60588091-60588110 Msa1204780:CDS 40.0%
TCCCTTAGTCATCTACGAAA+CGG + chr8_1:60588095-60588114 None:intergenic 40.0%
!! CGGTTCATAGTTCTAGCTCA+CGG - chr8_1:60586159-60586178 Msa1204780:three_prime_UTR 45.0%
!! GGTTCATAGTTCTAGCTCAC+GGG - chr8_1:60586160-60586179 Msa1204780:three_prime_UTR 45.0%
!! ATAGTTCTAGCTCACGGGTT+TGG - chr8_1:60586165-60586184 Msa1204780:three_prime_UTR 45.0%
CATCTAAGAGATGAGGTACG+AGG + chr8_1:60586215-60586234 None:intergenic 45.0%
CTTTATGACAACATGGGAGC+TGG - chr8_1:60586249-60586268 Msa1204780:three_prime_UTR 45.0%
AAAAAGACACCACCAAAGGG+TGG + chr8_1:60586712-60586731 None:intergenic 45.0%
TGAGTGTGTGTGTGTGTGTA+TGG - chr8_1:60587417-60587436 Msa1204780:intron 45.0%
! GTAAATGATGGGGGGATTTG+GGG - chr8_1:60587517-60587536 Msa1204780:intron 45.0%
!! GGGAAGGTATTTGAACGACA+GGG - chr8_1:60587537-60587556 Msa1204780:intron 45.0%
TTGAACGACAGGGACTACTT+TGG - chr8_1:60587547-60587566 Msa1204780:intron 45.0%
! GTGTTCTGGATTTGCTCCAA+TGG - chr8_1:60587633-60587652 Msa1204780:intron 45.0%
TATGAAGGATCTAGCGGTTC+CGG - chr8_1:60587816-60587835 Msa1204780:intron 45.0%
ATCACCGGAATAACAACGTC+CGG + chr8_1:60587934-60587953 None:intergenic 45.0%
TGCGGCTTGATGTTAATGTG+TGG - chr8_1:60587978-60587997 Msa1204780:CDS 45.0%
GTCCGTTTCGTAGATGACTA+AGG - chr8_1:60588090-60588109 Msa1204780:CDS 45.0%
TCGTAGATGACTAAGGGAGA+TGG - chr8_1:60588097-60588116 Msa1204780:CDS 45.0%
CGTAGATGACTAAGGGAGAT+GGG - chr8_1:60588098-60588117 Msa1204780:CDS 45.0%
GTAGATGACTAAGGGAGATG+GGG - chr8_1:60588099-60588118 Msa1204780:CDS 45.0%
! TCCTTGTTGTAATGTGGGGA+TGG - chr8_1:60588259-60588278 Msa1204780:five_prime_UTR 45.0%
TCCATCCCCACATTACAACA+AGG + chr8_1:60588263-60588282 None:intergenic 45.0%
TTGTTGTAATGTGGGGATGG+AGG - chr8_1:60588262-60588281 Msa1204780:five_prime_UTR 45.0%
!!! AATTTTTAATGTTAAATTAT+AGG - chr8_1:60586673-60586692 Msa1204780:CDS 5.0%
!!! ATTTTTAATGTTAAATTATA+GGG - chr8_1:60586674-60586693 Msa1204780:CDS 5.0%
!! AAAATCAATATTATAATTTA+GGG + chr8_1:60586884-60586903 None:intergenic 5.0%
!! TAAAATCAATATTATAATTT+AGG + chr8_1:60586885-60586904 None:intergenic 5.0%
!!! TAAATTATAATATTGATTTT+AGG - chr8_1:60586884-60586903 Msa1204780:intron 5.0%
!!! AAATATTAATTTTATTCTTA+AGG - chr8_1:60587220-60587239 Msa1204780:intron 5.0%
TTGGTTAAGGGGACTTGGGA+GGG + chr8_1:60586018-60586037 None:intergenic 50.0%
ATTGGTTAAGGGGACTTGGG+AGG + chr8_1:60586019-60586038 None:intergenic 50.0%
TGTTACAAGCCACCCTTTGG+TGG - chr8_1:60586700-60586719 Msa1204780:CDS 50.0%
!! GGGGAAGGTATTTGAACGAC+AGG - chr8_1:60587536-60587555 Msa1204780:intron 50.0%
AACGACAGGGACTACTTTGG+AGG - chr8_1:60587550-60587569 Msa1204780:intron 50.0%
! GGATTTGCTCCAATGGCAGT+TGG - chr8_1:60587640-60587659 Msa1204780:intron 50.0%
TTTGCTCCAATGGCAGTTGG+AGG - chr8_1:60587643-60587662 Msa1204780:intron 50.0%
TTGCTCCAATGGCAGTTGGA+GGG - chr8_1:60587644-60587663 Msa1204780:intron 50.0%
AACACGCTTAGGGCTATGTC+TGG + chr8_1:60587724-60587743 None:intergenic 50.0%
ACTCGCTACCAACATGTTGG+TGG + chr8_1:60587861-60587880 None:intergenic 50.0%
TGGACTCGCTACCAACATGT+TGG + chr8_1:60587864-60587883 None:intergenic 50.0%
TTTGCCGCAATTAAGCTCGC+CGG - chr8_1:60587912-60587931 Msa1204780:CDS 50.0%
ATGACTAAGGGAGATGGGGT+TGG - chr8_1:60588103-60588122 Msa1204780:CDS 50.0%
! ATGATGGGGGGATTTGGGGA+AGG - chr8_1:60587521-60587540 Msa1204780:intron 55.0%
TGCTCCAATGGCAGTTGGAG+GGG - chr8_1:60587645-60587664 Msa1204780:intron 55.0%
ATGTCCCCTCCAACTGCCAT+TGG + chr8_1:60587652-60587671 None:intergenic 55.0%
AATGGCAGTTGGAGGGGACA+TGG - chr8_1:60587651-60587670 Msa1204780:intron 55.0%
AGATATTCCGCGACCACGAC+CGG + chr8_1:60587838-60587857 None:intergenic 55.0%
!! TGTTGGTAGCGAGTCCATCG+TGG - chr8_1:60587867-60587886 Msa1204780:intron 55.0%
TGTCGACGTAACCTCCACGA+TGG + chr8_1:60587884-60587903 None:intergenic 55.0%
GTGGAGGTTACGTCGACAGA+GGG - chr8_1:60587886-60587905 Msa1204780:CDS 55.0%
ACGTCCGGCGAGCTTAATTG+CGG + chr8_1:60587919-60587938 None:intergenic 55.0%
! CTCGCCGGACGTTGTTATTC+CGG - chr8_1:60587927-60587946 Msa1204780:CDS 55.0%
GTTAAGGGGACTTGGGAGGG+TGG + chr8_1:60586015-60586034 None:intergenic 60.0%
! TGGTAGCGAGTCCATCGTGG+AGG - chr8_1:60587870-60587889 Msa1204780:intron 60.0%
CGTGGAGGTTACGTCGACAG+AGG - chr8_1:60587885-60587904 Msa1204780:CDS 60.0%
GGATCTAGCGGTTCCGGTCG+TGG - chr8_1:60587822-60587841 Msa1204780:intron 65.0%
AGCGGTTCCGGTCGTGGTCG+CGG - chr8_1:60587828-60587847 Msa1204780:intron 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 60585924 60588432 60585924 ID=Msa1204780;Name=Msa1204780
chr8_1 mRNA 60585924 60588432 60585924 ID=Msa1204780-mRNA-1;Parent=Msa1204780;Name=Msa1204780-mRNA-1;_AED=0.00;_eAED=0.00;_QI=184|1|1|1|0|0.5|2|445|270
chr8_1 exon 60585924 60586813 60585924 ID=Msa1204780-mRNA-1:exon:19657;Parent=Msa1204780-mRNA-1
chr8_1 exon 60587881 60588432 60587881 ID=Msa1204780-mRNA-1:exon:19656;Parent=Msa1204780-mRNA-1
chr8_1 five_prime_UTR 60588249 60588432 60588249 ID=Msa1204780-mRNA-1:five_prime_utr;Parent=Msa1204780-mRNA-1
chr8_1 CDS 60587881 60588248 60587881 ID=Msa1204780-mRNA-1:cds;Parent=Msa1204780-mRNA-1
chr8_1 CDS 60586369 60586813 60586369 ID=Msa1204780-mRNA-1:cds;Parent=Msa1204780-mRNA-1
chr8_1 three_prime_UTR 60585924 60586368 60585924 ID=Msa1204780-mRNA-1:three_prime_utr;Parent=Msa1204780-mRNA-1
Gene Sequence

>Msa1204780

ATGGGGATAGTAGAAGAAGCTCACAACGTGAAAGTTTTAGGATCAGGGTCACGGTTCATAGTTCTAGCTCACGGGTTTGGCACAGATCAATCTGTATGGAAACACCTCGTACCTCATCTCTTAGATGAGTTTCGTGTCATACTTTATGACAACATGGGAGCTGGTACAACTAATCCAGATTACTTTGATTTTGAGCGTTATTCAACCTTAGAAGGTTATGCTTATGATTTACTTGCAATCTTAGAAGAACTTCGAGTTGATTCATGTATCTTTGTTGGTCATTCTGTTTCTGCTATGATTGGAACTGTTGCATCAATTTCTCGACCTGATCTATTCAACAAAATTATTTTGATATCAGCTTCTCCAAGGTATTTGAACGACAGGGACTACTTTGGAGGATTTGAGCAAGAAGATTTGGATCAACTATTCGATGCTATGGCATCAAATTACAAATCATGGTGTTCTGGATTTGCTCCAATGGCAGTTGGAGGGGACATGGAGTCAGTAGCAGTACAAGAATTCAGTCGAACTTTGTTCAACATGAGACCAGACATAGCCCTAAGCGTGTTACAAACAATTTTTAAAAGTGACATGAGACAAATATTATGCATGGTCACTGTCCCATGCCACATTATACAAAGTATGAAGGATCTAGCGGTTCCGGTCGTGGTCGCGGAATATCTCCACCAACATGTTGGTAGCGAGTCCATCGTGGAGGTTACGTCGACAGAGGGTCATTTGCCGCAATTAAGCTCGCCGGACGTTGTTATTCCGGTGATACTCAAACACATTCGTCATGATATTGCGGCTTGA
Protein sequence

>Msa1204780

MGIVEEAHNVKVLGSGSRFIVLAHGFGTDQSVWKHLVPHLLDEFRVILYDNMGAGTTNPDYFDFERYSTLEGYAYDLLAILEELRVDSCIFVGHSVSAMIGTVASISRPDLFNKIILISASPRYLNDRDYFGGFEQEDLDQLFDAMASNYKSWCSGFAPMAVGGDMESVAVQEFSRTLFNMRPDIALSVLQTIFKSDMRQILCMVTVPCHIIQSMKDLAVPVVVAEYLHQHVGSESIVEVTSTEGHLPQLSSPDVVIPVILKHIRHDIAA*