AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1329390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1329390 MtrunA17_Chr5g0401521 80.224 268 53 0 1 268 1 268 4.52e-170 472
Msa1329390 MtrunA17_Chr4g0053741 100.000 219 0 0 52 270 1 219 6.38e-164 453
Msa1329390 MtrunA17_Chr1g0152551 57.430 249 104 2 6 253 8 255 8.00e-108 313
Msa1329390 MtrunA17_Chr3g0093821 40.408 245 144 2 9 252 13 256 2.55e-69 215
Msa1329390 MtrunA17_Chr6g0485021 40.157 254 149 3 9 260 22 274 1.71e-66 208
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1329390 AT4G37470.1 78.810 269 57 0 1 269 1 269 4.81e-166 461
Msa1329390 AT3G03990.1 57.661 248 104 1 6 253 8 254 2.97e-108 314
Msa1329390 AT3G24420.1 43.548 248 137 3 7 252 13 259 3.13e-76 233

Find 87 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGACCACGTCGTCATATTT+TGG 0.182565 8_4:+47975257 None:intergenic
TCATCCATAGTTTCTTAATT+AGG 0.267045 8_4:-47975281 Msa1329390:five_prime_UTR
AGCTCACAACGTGAAAGTTT+TGG 0.271854 8_4:-47975080 Msa1329390:CDS
TTGGATTAATCAGTAACTTT+AGG 0.290550 8_4:+47975128 None:intergenic
TGGACCTAATTAAGAAACTA+TGG 0.290707 8_4:+47975277 None:intergenic
AGGATTTGAGCAAGAAGATT+TGG 0.309415 8_4:-47973837 Msa1329390:CDS
CTCGCCGGACGTTGTTATTC+CGG 0.318614 8_4:-47973480 Msa1329390:CDS
GTTGCATGATTGTATTATTT+AGG 0.322130 8_4:-47973398 Msa1329390:three_prime_UTR
CGCTCAAAATCAAAGTAATC+TGG 0.326618 8_4:+47974923 None:intergenic
CATTCTGTTTCTGCTATGAT+TGG 0.327046 8_4:-47974818 Msa1329390:CDS
GAGCGTTATTCAACCTTAGA+AGG 0.329474 8_4:-47974905 Msa1329390:CDS
AATTACAAATCATGGTGTTC+TGG 0.329664 8_4:-47973788 Msa1329390:CDS
GTTGATTCATGTATCTTTGT+TGG 0.344178 8_4:-47974842 Msa1329390:CDS
GACGACGTGGTCTAAAACTT+TGG 0.346914 8_4:-47975248 Msa1329390:five_prime_UTR
AAATTGTTTGTAACACGCTT+AGG 0.357437 8_4:+47973675 None:intergenic
ATCATAAGCATAACCTTCTA+AGG 0.361019 8_4:+47974892 None:intergenic
GTTAAGGGGACTTGGGAGGG+TGG 0.362712 8_4:+47975193 None:intergenic
TAACAACAATACATAATTGA+TGG 0.378492 8_4:+47973270 None:intergenic
ATAGTTCTAGCTCACGGGTT+TGG 0.396140 8_4:-47975040 Msa1329390:CDS
TTGGTTAAGGGGACTTGGGA+GGG 0.411587 8_4:+47975190 None:intergenic
AATTGTTTGTAACACGCTTA+GGG 0.419608 8_4:+47973676 None:intergenic
AGATATTCCGCGACCACGAC+TGG 0.422541 8_4:+47973572 None:intergenic
TAGATGCTCATAATTATTGA+TGG 0.435669 8_4:+47973208 None:intergenic
AAACTATGGATGATAGCAAC+CGG 0.435693 8_4:+47975291 None:intergenic
TGGCATCAAATTACAAATCA+TGG 0.445032 8_4:-47973796 Msa1329390:CDS
AACGACAGGGACTACTTTGG+AGG 0.459003 8_4:-47973857 Msa1329390:CDS
GGATTTGCTCCAATGGCAGT+TGG 0.462285 8_4:-47973767 Msa1329390:CDS
ATGTCCCCTCCAACTGCCAT+TGG 0.469708 8_4:+47973758 None:intergenic
GCCTCCATCCCCACATTACA+AGG 0.471192 8_4:+47973148 None:intergenic
GGATCAACTATTCGATGCTA+TGG 0.479394 8_4:-47973816 Msa1329390:CDS
GTCCGTTTCGTAGATGACTA+AGG 0.483932 8_4:-47973318 Msa1329390:three_prime_UTR
CGTAGATGACTAAGGGAGAT+GGG 0.485855 8_4:-47973310 Msa1329390:three_prime_UTR
TTGAACGACAGGGACTACTT+TGG 0.492694 8_4:-47973860 Msa1329390:CDS
GGGGAAGGTATTTGAACGAC+AGG 0.494683 8_4:-47973871 Msa1329390:intron
TGCTCCAATGGCAGTTGGAG+GGG 0.510556 8_4:-47973762 Msa1329390:CDS
CCTTCATACTTTGTATAATG+TGG 0.518604 8_4:+47973606 None:intergenic
TACTTTGTATAATGTGGCAT+GGG 0.519899 8_4:+47973612 None:intergenic
TTTGGTTAAGGGGACTTGGG+AGG 0.525703 8_4:+47975189 None:intergenic
TCGTAGATGACTAAGGGAGA+TGG 0.526663 8_4:-47973311 Msa1329390:three_prime_UTR
AATGGCAGTTGGAGGGGACA+TGG 0.527300 8_4:-47973756 Msa1329390:CDS
TCCCTTAGTCATCTACGAAA+CGG 0.530875 8_4:+47973316 None:intergenic
GTGTTCTGGATTTGCTCCAA+TGG 0.532527 8_4:-47973774 Msa1329390:CDS
GGTTCATAGTTCTAGCTCAC+GGG 0.534969 8_4:-47975045 Msa1329390:CDS
TTGGCACAGATCAATCTGTA+TGG 0.535156 8_4:-47975021 Msa1329390:CDS
AGCGGTTCCAGTCGTGGTCG+CGG 0.535585 8_4:-47973579 Msa1329390:CDS
ATCACCGGAATAACAACGTC+CGG 0.537857 8_4:+47973476 None:intergenic
CCACATTATACAAAGTATGA+AGG 0.538729 8_4:-47973606 Msa1329390:CDS
TTTGCTCCAATGGCAGTTGG+AGG 0.538790 8_4:-47973764 Msa1329390:CDS
ATACTTTGTATAATGTGGCA+TGG 0.539342 8_4:+47973611 None:intergenic
AAAATGAAAGAGCATGAAAG+AGG 0.542605 8_4:-47973357 Msa1329390:three_prime_UTR
CTTTATGACAACATGGGAGC+TGG 0.543266 8_4:-47974956 Msa1329390:CDS
TTATCCTTGTAATGTGGGGA+TGG 0.544383 8_4:-47973152 Msa1329390:three_prime_UTR
TTGCTCCAATGGCAGTTGGA+GGG 0.545271 8_4:-47973763 Msa1329390:CDS
CGAATGTGTTTGAGTATCAC+CGG 0.546328 8_4:+47973461 None:intergenic
ATGTTAATGTGTGGTAGGTG+TGG 0.549229 8_4:-47973420 Msa1329390:three_prime_UTR
GTGTTGTTATCCTTGTAATG+TGG 0.555830 8_4:-47973158 Msa1329390:three_prime_UTR
CGGTTCATAGTTCTAGCTCA+CGG 0.558590 8_4:-47975046 Msa1329390:CDS
TGTTGTTATCCTTGTAATGT+GGG 0.561522 8_4:-47973157 Msa1329390:three_prime_UTR
AACACGCTTAGGGCTATGTC+TGG 0.562881 8_4:+47973686 None:intergenic
AAATGAAAGAGCATGAAAGA+GGG 0.566913 8_4:-47973356 Msa1329390:three_prime_UTR
TCCTTGTAATGTGGGGATGG+AGG 0.573982 8_4:-47973149 Msa1329390:three_prime_UTR
ACTCGCTACCAACATGTTGG+TGG 0.576429 8_4:+47973549 None:intergenic
ATGACTAAGGGAGATGGGGT+TGG 0.576791 8_4:-47973305 Msa1329390:three_prime_UTR
GGATCTAGCGGTTCCAGTCG+TGG 0.576985 8_4:-47973585 Msa1329390:CDS
TCCGTTTCGTAGATGACTAA+GGG 0.581710 8_4:-47973317 Msa1329390:three_prime_UTR
ACACATTCGTCATGATATTG+CGG 0.602861 8_4:-47973447 Msa1329390:CDS
GAATATCTCCACCAACATGT+TGG 0.605480 8_4:-47973557 Msa1329390:CDS
GCTTGATGTTAATGTGTGGT+AGG 0.607429 8_4:-47973425 Msa1329390:exon
TGGACTCGCTACCAACATGT+TGG 0.613943 8_4:+47973546 None:intergenic
CATGAGACAAATATTATGCA+TGG 0.615267 8_4:-47973642 Msa1329390:CDS
TTTGCCGCAATTAAGCTCGC+CGG 0.620386 8_4:-47973495 Msa1329390:CDS
TGTCATACTTTATGACAACA+TGG 0.621368 8_4:-47974963 Msa1329390:CDS
GTGGAGGTTATGTCGACAGA+GGG 0.648761 8_4:-47973521 Msa1329390:CDS
AACTATGGATGATAGCAACC+GGG 0.648957 8_4:+47975292 None:intergenic
CGAAACTCATCTAAGAGATG+AGG 0.649391 8_4:+47974986 None:intergenic
GTTGTTATCCTTGTAATGTG+GGG 0.654182 8_4:-47973156 Msa1329390:three_prime_UTR
ACAAAGTATGAAGGATCTAG+CGG 0.659829 8_4:-47973597 Msa1329390:CDS
GGGAAGGTATTTGAACGACA+GGG 0.664911 8_4:-47973870 Msa1329390:intron
GTAGATGACTAAGGGAGATG+GGG 0.666306 8_4:-47973309 Msa1329390:three_prime_UTR
GTCATACTTTATGACAACAT+GGG 0.668133 8_4:-47974962 Msa1329390:CDS
CGTGGAGGTTATGTCGACAG+AGG 0.671230 8_4:-47973522 Msa1329390:CDS
CATCTAAGAGATGAGGTACG+AGG 0.688542 8_4:+47974993 None:intergenic
TGCGGCTTGATGTTAATGTG+TGG 0.709045 8_4:-47973429 Msa1329390:exon
TGTTGGTAGCGAGTCCATCG+TGG 0.709388 8_4:-47973540 Msa1329390:CDS
TGGTAGCGAGTCCATCGTGG+AGG 0.724029 8_4:-47973537 Msa1329390:CDS
TGTCGACATAACCTCCACGA+TGG 0.724152 8_4:+47973526 None:intergenic
AGGTCCAAAATATGACGACG+TGG 0.734949 8_4:-47975261 Msa1329390:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTAGTCCAATATTTA+TGG + chr8_4:47973892-47973911 None:intergenic 15.0%
!!! TAAATATCATATATTTTGAG+TGG - chr8_4:47974120-47974139 Msa1329390:intron 15.0%
!!! TTTTGATCATTATATTATGT+AGG - chr8_4:47974181-47974200 Msa1329390:intron 15.0%
!! TAATGTATCAATAGTTAATA+AGG - chr8_4:47974306-47974325 Msa1329390:intron 15.0%
!! ATTATTAATGATGTAAATGA+TGG - chr8_4:47974536-47974555 Msa1329390:intron 15.0%
!! TTATTAATGATGTAAATGAT+GGG - chr8_4:47974537-47974556 Msa1329390:intron 15.0%
!! AAAAATTAGTCCAATATTTA+TGG + chr8_4:47973892-47973911 None:intergenic 15.0%
!!! TAAATATCATATATTTTGAG+TGG - chr8_4:47974120-47974139 Msa1329390:intron 15.0%
!!! TTTTGATCATTATATTATGT+AGG - chr8_4:47974181-47974200 Msa1329390:intron 15.0%
!! TAATGTATCAATAGTTAATA+AGG - chr8_4:47974306-47974325 Msa1329390:intron 15.0%
!! ATTATTAATGATGTAAATGA+TGG - chr8_4:47974536-47974555 Msa1329390:intron 15.0%
!! TTATTAATGATGTAAATGAT+GGG - chr8_4:47974537-47974556 Msa1329390:intron 15.0%
!!! TTTTTCTTTGTTTTGGTTAA+GGG + chr8_4:47973262-47973281 None:intergenic 20.0%
!!! GTATTTGTTTTTCTTTGTTT+TGG + chr8_4:47973269-47973288 None:intergenic 20.0%
!! ATTAATCCAACACAAAAAAT+GGG - chr8_4:47973322-47973341 Msa1329390:three_prime_UTR 20.0%
!!! ATAATTTTGTTGAATAGATC+AGG + chr8_4:47973667-47973686 None:intergenic 20.0%
!! GATTAATCATTGTACAATAA+TGG - chr8_4:47973784-47973803 Msa1329390:CDS 20.0%
!!! TTTTTTGTCTCCATAAATAT+TGG - chr8_4:47973879-47973898 Msa1329390:intron 20.0%
!! GATAAAAATTATCTCTTGAA+AGG + chr8_4:47974036-47974055 None:intergenic 20.0%
!! ATTATATTATGTAGGCATAT+TGG - chr8_4:47974189-47974208 Msa1329390:intron 20.0%
!!! TTTTTTCTTTCTTTCATCTT+TGG - chr8_4:47974369-47974388 Msa1329390:intron 20.0%
!! TATTAATGATGTAAATGATG+GGG - chr8_4:47974538-47974557 Msa1329390:intron 20.0%
!! TAACAACAATACATAATTGA+TGG + chr8_4:47975170-47975189 None:intergenic 20.0%
!!! GTTATCCTTTTTTTTACATA+AGG - chr8_4:47975257-47975276 Msa1329390:five_prime_UTR 20.0%
!!! TTTTTCTTTGTTTTGGTTAA+GGG + chr8_4:47973262-47973281 None:intergenic 20.0%
!!! GTATTTGTTTTTCTTTGTTT+TGG + chr8_4:47973269-47973288 None:intergenic 20.0%
!! ATTAATCCAACACAAAAAAT+GGG - chr8_4:47973322-47973341 Msa1329390:three_prime_UTR 20.0%
!!! ATAATTTTGTTGAATAGATC+AGG + chr8_4:47973667-47973686 None:intergenic 20.0%
!! GATTAATCATTGTACAATAA+TGG - chr8_4:47973784-47973803 Msa1329390:CDS 20.0%
!!! TTTTTTGTCTCCATAAATAT+TGG - chr8_4:47973879-47973898 Msa1329390:intron 20.0%
!! GATAAAAATTATCTCTTGAA+AGG + chr8_4:47974036-47974055 None:intergenic 20.0%
!! ATTATATTATGTAGGCATAT+TGG - chr8_4:47974189-47974208 Msa1329390:intron 20.0%
!!! TTTTTTCTTTCTTTCATCTT+TGG - chr8_4:47974369-47974388 Msa1329390:intron 20.0%
!! TATTAATGATGTAAATGATG+GGG - chr8_4:47974538-47974557 Msa1329390:intron 20.0%
!! TAACAACAATACATAATTGA+TGG + chr8_4:47975170-47975189 None:intergenic 20.0%
!!! GTTATCCTTTTTTTTACATA+AGG - chr8_4:47975257-47975276 Msa1329390:five_prime_UTR 20.0%
! TCATCCATAGTTTCTTAATT+AGG - chr8_4:47973156-47973175 Msa1329390:three_prime_UTR 25.0%
!!! TTTTCTTTGTTTTGGTTAAG+GGG + chr8_4:47973261-47973280 None:intergenic 25.0%
!!! GTTTTTCTTTGTTTTGGTTA+AGG + chr8_4:47973263-47973282 None:intergenic 25.0%
! TTGGATTAATCAGTAACTTT+AGG + chr8_4:47973312-47973331 None:intergenic 25.0%
! GATTAATCCAACACAAAAAA+TGG - chr8_4:47973321-47973340 Msa1329390:three_prime_UTR 25.0%
! TTAATCCAACACAAAAAATG+GGG - chr8_4:47973323-47973342 Msa1329390:three_prime_UTR 25.0%
!!! AATTTAATTTGACCATACCT+TGG + chr8_4:47973706-47973725 None:intergenic 25.0%
!! TCAACAAAGTCTTATATTTG+AGG + chr8_4:47973982-47974001 None:intergenic 25.0%
! TGATCAAAATAAAACTGCAT+AGG + chr8_4:47974170-47974189 None:intergenic 25.0%
!!! TAGTGCATCCTTTTTTTTAA+AGG + chr8_4:47974337-47974356 None:intergenic 25.0%
! ATTAATGATGTAAATGATGG+GGG - chr8_4:47974539-47974558 Msa1329390:intron 25.0%
! GTTGCATGATTGTATTATTT+AGG - chr8_4:47975039-47975058 Msa1329390:CDS 25.0%
!!! TATGCTAGTGTTTTTAAGTT+TGG - chr8_4:47975194-47975213 Msa1329390:five_prime_UTR 25.0%
! TAGATGCTCATAATTATTGA+TGG + chr8_4:47975232-47975251 None:intergenic 25.0%
! CAACACCTTATGTAAAAAAA+AGG + chr8_4:47975265-47975284 None:intergenic 25.0%
! TCATCCATAGTTTCTTAATT+AGG - chr8_4:47973156-47973175 Msa1329390:three_prime_UTR 25.0%
!!! TTTTCTTTGTTTTGGTTAAG+GGG + chr8_4:47973261-47973280 None:intergenic 25.0%
!!! GTTTTTCTTTGTTTTGGTTA+AGG + chr8_4:47973263-47973282 None:intergenic 25.0%
! TTGGATTAATCAGTAACTTT+AGG + chr8_4:47973312-47973331 None:intergenic 25.0%
! GATTAATCCAACACAAAAAA+TGG - chr8_4:47973321-47973340 Msa1329390:three_prime_UTR 25.0%
! TTAATCCAACACAAAAAATG+GGG - chr8_4:47973323-47973342 Msa1329390:three_prime_UTR 25.0%
!!! AATTTAATTTGACCATACCT+TGG + chr8_4:47973706-47973725 None:intergenic 25.0%
!! TCAACAAAGTCTTATATTTG+AGG + chr8_4:47973982-47974001 None:intergenic 25.0%
! TGATCAAAATAAAACTGCAT+AGG + chr8_4:47974170-47974189 None:intergenic 25.0%
!!! TAGTGCATCCTTTTTTTTAA+AGG + chr8_4:47974337-47974356 None:intergenic 25.0%
! ATTAATGATGTAAATGATGG+GGG - chr8_4:47974539-47974558 Msa1329390:intron 25.0%
! GTTGCATGATTGTATTATTT+AGG - chr8_4:47975039-47975058 Msa1329390:CDS 25.0%
!!! TATGCTAGTGTTTTTAAGTT+TGG - chr8_4:47975194-47975213 Msa1329390:five_prime_UTR 25.0%
! TAGATGCTCATAATTATTGA+TGG + chr8_4:47975232-47975251 None:intergenic 25.0%
! CAACACCTTATGTAAAAAAA+AGG + chr8_4:47975265-47975284 None:intergenic 25.0%
TGGACCTAATTAAGAAACTA+TGG + chr8_4:47973163-47973182 None:intergenic 30.0%
TGTCATACTTTATGACAACA+TGG - chr8_4:47973474-47973493 Msa1329390:CDS 30.0%
GTCATACTTTATGACAACAT+GGG - chr8_4:47973475-47973494 Msa1329390:CDS 30.0%
ATCATAAGCATAACCTTCTA+AGG + chr8_4:47973548-47973567 None:intergenic 30.0%
! GTTGATTCATGTATCTTTGT+TGG - chr8_4:47973595-47973614 Msa1329390:CDS 30.0%
! AGGATGCACCTTTAAAAAAA+AGG - chr8_4:47974326-47974345 Msa1329390:intron 30.0%
TGGCATCAAATTACAAATCA+TGG - chr8_4:47974641-47974660 Msa1329390:intron 30.0%
AATTACAAATCATGGTGTTC+TGG - chr8_4:47974649-47974668 Msa1329390:intron 30.0%
AATTGTTTGTAACACGCTTA+GGG + chr8_4:47974764-47974783 None:intergenic 30.0%
AAATTGTTTGTAACACGCTT+AGG + chr8_4:47974765-47974784 None:intergenic 30.0%
CATGAGACAAATATTATGCA+TGG - chr8_4:47974795-47974814 Msa1329390:CDS 30.0%
TACTTTGTATAATGTGGCAT+GGG + chr8_4:47974828-47974847 None:intergenic 30.0%
ATACTTTGTATAATGTGGCA+TGG + chr8_4:47974829-47974848 None:intergenic 30.0%
CCTTCATACTTTGTATAATG+TGG + chr8_4:47974834-47974853 None:intergenic 30.0%
CCACATTATACAAAGTATGA+AGG - chr8_4:47974831-47974850 Msa1329390:CDS 30.0%
! AAAATGAAAGAGCATGAAAG+AGG - chr8_4:47975080-47975099 Msa1329390:CDS 30.0%
! AAATGAAAGAGCATGAAAGA+GGG - chr8_4:47975081-47975100 Msa1329390:CDS 30.0%
!!! CTAGTGTTTTTAAGTTTGGT+AGG - chr8_4:47975198-47975217 Msa1329390:five_prime_UTR 30.0%
TGTTGTTATCCTTGTAATGT+GGG - chr8_4:47975280-47975299 Msa1329390:five_prime_UTR 30.0%
TGGACCTAATTAAGAAACTA+TGG + chr8_4:47973163-47973182 None:intergenic 30.0%
TGTCATACTTTATGACAACA+TGG - chr8_4:47973474-47973493 Msa1329390:CDS 30.0%
GTCATACTTTATGACAACAT+GGG - chr8_4:47973475-47973494 Msa1329390:CDS 30.0%
ATCATAAGCATAACCTTCTA+AGG + chr8_4:47973548-47973567 None:intergenic 30.0%
! GTTGATTCATGTATCTTTGT+TGG - chr8_4:47973595-47973614 Msa1329390:CDS 30.0%
! AGGATGCACCTTTAAAAAAA+AGG - chr8_4:47974326-47974345 Msa1329390:intron 30.0%
TGGCATCAAATTACAAATCA+TGG - chr8_4:47974641-47974660 Msa1329390:intron 30.0%
AATTACAAATCATGGTGTTC+TGG - chr8_4:47974649-47974668 Msa1329390:intron 30.0%
AATTGTTTGTAACACGCTTA+GGG + chr8_4:47974764-47974783 None:intergenic 30.0%
AAATTGTTTGTAACACGCTT+AGG + chr8_4:47974765-47974784 None:intergenic 30.0%
CATGAGACAAATATTATGCA+TGG - chr8_4:47974795-47974814 Msa1329390:CDS 30.0%
TACTTTGTATAATGTGGCAT+GGG + chr8_4:47974828-47974847 None:intergenic 30.0%
ATACTTTGTATAATGTGGCA+TGG + chr8_4:47974829-47974848 None:intergenic 30.0%
CCTTCATACTTTGTATAATG+TGG + chr8_4:47974834-47974853 None:intergenic 30.0%
CCACATTATACAAAGTATGA+AGG - chr8_4:47974831-47974850 Msa1329390:CDS 30.0%
! AAAATGAAAGAGCATGAAAG+AGG - chr8_4:47975080-47975099 Msa1329390:CDS 30.0%
! AAATGAAAGAGCATGAAAGA+GGG - chr8_4:47975081-47975100 Msa1329390:CDS 30.0%
!!! CTAGTGTTTTTAAGTTTGGT+AGG - chr8_4:47975198-47975217 Msa1329390:five_prime_UTR 30.0%
TGTTGTTATCCTTGTAATGT+GGG - chr8_4:47975280-47975299 Msa1329390:five_prime_UTR 30.0%
AAACTATGGATGATAGCAAC+CGG + chr8_4:47973149-47973168 None:intergenic 35.0%
!!! ACTATCCCCATTTTTTGTGT+TGG + chr8_4:47973331-47973350 None:intergenic 35.0%
CGCTCAAAATCAAAGTAATC+TGG + chr8_4:47973517-47973536 None:intergenic 35.0%
!! CATTCTGTTTCTGCTATGAT+TGG - chr8_4:47973619-47973638 Msa1329390:CDS 35.0%
! TTTTGATATCAGCTTCTCCA+AGG - chr8_4:47973686-47973705 Msa1329390:CDS 35.0%
! ATGTAAATGATGGGGGATTT+GGG - chr8_4:47974546-47974565 Msa1329390:intron 35.0%
AGGATTTGAGCAAGAAGATT+TGG - chr8_4:47974600-47974619 Msa1329390:intron 35.0%
ACAAAGTATGAAGGATCTAG+CGG - chr8_4:47974840-47974859 Msa1329390:CDS 35.0%
ACACATTCGTCATGATATTG+CGG - chr8_4:47974990-47975009 Msa1329390:CDS 35.0%
!!! GTGTTTTTAAGTTTGGTAGG+TGG - chr8_4:47975201-47975220 Msa1329390:five_prime_UTR 35.0%
! GTGTTGTTATCCTTGTAATG+TGG - chr8_4:47975279-47975298 Msa1329390:five_prime_UTR 35.0%
GTTGTTATCCTTGTAATGTG+GGG - chr8_4:47975281-47975300 Msa1329390:five_prime_UTR 35.0%
AAACTATGGATGATAGCAAC+CGG + chr8_4:47973149-47973168 None:intergenic 35.0%
!!! ACTATCCCCATTTTTTGTGT+TGG + chr8_4:47973331-47973350 None:intergenic 35.0%
CGCTCAAAATCAAAGTAATC+TGG + chr8_4:47973517-47973536 None:intergenic 35.0%
!! CATTCTGTTTCTGCTATGAT+TGG - chr8_4:47973619-47973638 Msa1329390:CDS 35.0%
! TTTTGATATCAGCTTCTCCA+AGG - chr8_4:47973686-47973705 Msa1329390:CDS 35.0%
! ATGTAAATGATGGGGGATTT+GGG - chr8_4:47974546-47974565 Msa1329390:intron 35.0%
AGGATTTGAGCAAGAAGATT+TGG - chr8_4:47974600-47974619 Msa1329390:intron 35.0%
ACAAAGTATGAAGGATCTAG+CGG - chr8_4:47974840-47974859 Msa1329390:CDS 35.0%
ACACATTCGTCATGATATTG+CGG - chr8_4:47974990-47975009 Msa1329390:CDS 35.0%
!!! GTGTTTTTAAGTTTGGTAGG+TGG - chr8_4:47975201-47975220 Msa1329390:five_prime_UTR 35.0%
! GTGTTGTTATCCTTGTAATG+TGG - chr8_4:47975279-47975298 Msa1329390:five_prime_UTR 35.0%
GTTGTTATCCTTGTAATGTG+GGG - chr8_4:47975281-47975300 Msa1329390:five_prime_UTR 35.0%
! TAGACCACGTCGTCATATTT+TGG + chr8_4:47973183-47973202 None:intergenic 40.0%
!!! TGTTTTGGTTAAGGGGACTT+GGG + chr8_4:47973254-47973273 None:intergenic 40.0%
!!! TTGTTTTGGTTAAGGGGACT+TGG + chr8_4:47973255-47973274 None:intergenic 40.0%
! AGCTCACAACGTGAAAGTTT+TGG - chr8_4:47973357-47973376 Msa1329390:three_prime_UTR 40.0%
! GCTCACAACGTGAAAGTTTT+GGG - chr8_4:47973358-47973377 Msa1329390:three_prime_UTR 40.0%
!!! AACGTGAAAGTTTTGGGTTC+AGG - chr8_4:47973364-47973383 Msa1329390:three_prime_UTR 40.0%
!!! ACGTGAAAGTTTTGGGTTCA+GGG - chr8_4:47973365-47973384 Msa1329390:three_prime_UTR 40.0%
TTGGCACAGATCAATCTGTA+TGG - chr8_4:47973416-47973435 Msa1329390:three_prime_UTR 40.0%
CGAAACTCATCTAAGAGATG+AGG + chr8_4:47973454-47973473 None:intergenic 40.0%
GAGCGTTATTCAACCTTAGA+AGG - chr8_4:47973532-47973551 Msa1329390:CDS 40.0%
ATATCAGCTTCTCCAAGGTA+TGG - chr8_4:47973691-47973710 Msa1329390:CDS 40.0%
AGTGAGTGTGTGTGTGTATA+TGG - chr8_4:47974448-47974467 Msa1329390:intron 40.0%
! GATGTAAATGATGGGGGATT+TGG - chr8_4:47974545-47974564 Msa1329390:intron 40.0%
! TGTAAATGATGGGGGATTTG+GGG - chr8_4:47974547-47974566 Msa1329390:intron 40.0%
GGATCAACTATTCGATGCTA+TGG - chr8_4:47974621-47974640 Msa1329390:intron 40.0%
GAATATCTCCACCAACATGT+TGG - chr8_4:47974880-47974899 Msa1329390:CDS 40.0%
CGAATGTGTTTGAGTATCAC+CGG + chr8_4:47974979-47974998 None:intergenic 40.0%
GCTTGATGTTAATGTGTGGT+AGG - chr8_4:47975012-47975031 Msa1329390:CDS 40.0%
ATGTTAATGTGTGGTAGGTG+TGG - chr8_4:47975017-47975036 Msa1329390:CDS 40.0%
TCCGTTTCGTAGATGACTAA+GGG - chr8_4:47975120-47975139 Msa1329390:five_prime_UTR 40.0%
TCCCTTAGTCATCTACGAAA+CGG + chr8_4:47975124-47975143 None:intergenic 40.0%
TTATCCTTGTAATGTGGGGA+TGG - chr8_4:47975285-47975304 Msa1329390:five_prime_UTR 40.0%
! TAGACCACGTCGTCATATTT+TGG + chr8_4:47973183-47973202 None:intergenic 40.0%
!!! TGTTTTGGTTAAGGGGACTT+GGG + chr8_4:47973254-47973273 None:intergenic 40.0%
!!! TTGTTTTGGTTAAGGGGACT+TGG + chr8_4:47973255-47973274 None:intergenic 40.0%
! AGCTCACAACGTGAAAGTTT+TGG - chr8_4:47973357-47973376 Msa1329390:three_prime_UTR 40.0%
! GCTCACAACGTGAAAGTTTT+GGG - chr8_4:47973358-47973377 Msa1329390:three_prime_UTR 40.0%
!!! AACGTGAAAGTTTTGGGTTC+AGG - chr8_4:47973364-47973383 Msa1329390:three_prime_UTR 40.0%
!!! ACGTGAAAGTTTTGGGTTCA+GGG - chr8_4:47973365-47973384 Msa1329390:three_prime_UTR 40.0%
TTGGCACAGATCAATCTGTA+TGG - chr8_4:47973416-47973435 Msa1329390:three_prime_UTR 40.0%
CGAAACTCATCTAAGAGATG+AGG + chr8_4:47973454-47973473 None:intergenic 40.0%
GAGCGTTATTCAACCTTAGA+AGG - chr8_4:47973532-47973551 Msa1329390:CDS 40.0%
ATATCAGCTTCTCCAAGGTA+TGG - chr8_4:47973691-47973710 Msa1329390:CDS 40.0%
AGTGAGTGTGTGTGTGTATA+TGG - chr8_4:47974448-47974467 Msa1329390:intron 40.0%
! GATGTAAATGATGGGGGATT+TGG - chr8_4:47974545-47974564 Msa1329390:intron 40.0%
! TGTAAATGATGGGGGATTTG+GGG - chr8_4:47974547-47974566 Msa1329390:intron 40.0%
GGATCAACTATTCGATGCTA+TGG - chr8_4:47974621-47974640 Msa1329390:intron 40.0%
GAATATCTCCACCAACATGT+TGG - chr8_4:47974880-47974899 Msa1329390:CDS 40.0%
CGAATGTGTTTGAGTATCAC+CGG + chr8_4:47974979-47974998 None:intergenic 40.0%
GCTTGATGTTAATGTGTGGT+AGG - chr8_4:47975012-47975031 Msa1329390:CDS 40.0%
ATGTTAATGTGTGGTAGGTG+TGG - chr8_4:47975017-47975036 Msa1329390:CDS 40.0%
TCCGTTTCGTAGATGACTAA+GGG - chr8_4:47975120-47975139 Msa1329390:five_prime_UTR 40.0%
TCCCTTAGTCATCTACGAAA+CGG + chr8_4:47975124-47975143 None:intergenic 40.0%
TTATCCTTGTAATGTGGGGA+TGG - chr8_4:47975285-47975304 Msa1329390:five_prime_UTR 40.0%
AGGTCCAAAATATGACGACG+TGG - chr8_4:47973176-47973195 Msa1329390:three_prime_UTR 45.0%
! GACGACGTGGTCTAAAACTT+TGG - chr8_4:47973189-47973208 Msa1329390:three_prime_UTR 45.0%
!!! AAGTTTTGGGTTCAGGGTCA+CGG - chr8_4:47973371-47973390 Msa1329390:three_prime_UTR 45.0%
!! CGGTTCATAGTTCTAGCTCA+CGG - chr8_4:47973391-47973410 Msa1329390:three_prime_UTR 45.0%
!! GGTTCATAGTTCTAGCTCAC+GGG - chr8_4:47973392-47973411 Msa1329390:three_prime_UTR 45.0%
!! ATAGTTCTAGCTCACGGGTT+TGG - chr8_4:47973397-47973416 Msa1329390:three_prime_UTR 45.0%
CATCTAAGAGATGAGGTACG+AGG + chr8_4:47973447-47973466 None:intergenic 45.0%
CTTTATGACAACATGGGAGC+TGG - chr8_4:47973481-47973500 Msa1329390:CDS 45.0%
!! GGGAAGGTATTTGAACGACA+GGG - chr8_4:47974567-47974586 Msa1329390:intron 45.0%
TTGAACGACAGGGACTACTT+TGG - chr8_4:47974577-47974596 Msa1329390:intron 45.0%
! GTGTTCTGGATTTGCTCCAA+TGG - chr8_4:47974663-47974682 Msa1329390:intron 45.0%
ATCACCGGAATAACAACGTC+CGG + chr8_4:47974964-47974983 None:intergenic 45.0%
TGCGGCTTGATGTTAATGTG+TGG - chr8_4:47975008-47975027 Msa1329390:CDS 45.0%
GTCCGTTTCGTAGATGACTA+AGG - chr8_4:47975119-47975138 Msa1329390:exon 45.0%
TCGTAGATGACTAAGGGAGA+TGG - chr8_4:47975126-47975145 Msa1329390:five_prime_UTR 45.0%
CGTAGATGACTAAGGGAGAT+GGG - chr8_4:47975127-47975146 Msa1329390:five_prime_UTR 45.0%
GTAGATGACTAAGGGAGATG+GGG - chr8_4:47975128-47975147 Msa1329390:five_prime_UTR 45.0%
AGGTCCAAAATATGACGACG+TGG - chr8_4:47973176-47973195 Msa1329390:three_prime_UTR 45.0%
! GACGACGTGGTCTAAAACTT+TGG - chr8_4:47973189-47973208 Msa1329390:three_prime_UTR 45.0%
!!! AAGTTTTGGGTTCAGGGTCA+CGG - chr8_4:47973371-47973390 Msa1329390:three_prime_UTR 45.0%
!! CGGTTCATAGTTCTAGCTCA+CGG - chr8_4:47973391-47973410 Msa1329390:three_prime_UTR 45.0%
!! GGTTCATAGTTCTAGCTCAC+GGG - chr8_4:47973392-47973411 Msa1329390:three_prime_UTR 45.0%
!! ATAGTTCTAGCTCACGGGTT+TGG - chr8_4:47973397-47973416 Msa1329390:three_prime_UTR 45.0%
CATCTAAGAGATGAGGTACG+AGG + chr8_4:47973447-47973466 None:intergenic 45.0%
CTTTATGACAACATGGGAGC+TGG - chr8_4:47973481-47973500 Msa1329390:CDS 45.0%
!! GGGAAGGTATTTGAACGACA+GGG - chr8_4:47974567-47974586 Msa1329390:intron 45.0%
TTGAACGACAGGGACTACTT+TGG - chr8_4:47974577-47974596 Msa1329390:intron 45.0%
! GTGTTCTGGATTTGCTCCAA+TGG - chr8_4:47974663-47974682 Msa1329390:intron 45.0%
ATCACCGGAATAACAACGTC+CGG + chr8_4:47974964-47974983 None:intergenic 45.0%
TGCGGCTTGATGTTAATGTG+TGG - chr8_4:47975008-47975027 Msa1329390:CDS 45.0%
GTCCGTTTCGTAGATGACTA+AGG - chr8_4:47975119-47975138 Msa1329390:exon 45.0%
TCGTAGATGACTAAGGGAGA+TGG - chr8_4:47975126-47975145 Msa1329390:five_prime_UTR 45.0%
CGTAGATGACTAAGGGAGAT+GGG - chr8_4:47975127-47975146 Msa1329390:five_prime_UTR 45.0%
GTAGATGACTAAGGGAGATG+GGG - chr8_4:47975128-47975147 Msa1329390:five_prime_UTR 45.0%
!!! TAAATTATAATATTGATTTT+AGG - chr8_4:47973917-47973936 Msa1329390:intron 5.0%
!!! AAATATTAATTTTATTCTTA+AGG - chr8_4:47974253-47974272 Msa1329390:intron 5.0%
!!! TAAATTATAATATTGATTTT+AGG - chr8_4:47973917-47973936 Msa1329390:intron 5.0%
!!! AAATATTAATTTTATTCTTA+AGG - chr8_4:47974253-47974272 Msa1329390:intron 5.0%
TTGGTTAAGGGGACTTGGGA+GGG + chr8_4:47973250-47973269 None:intergenic 50.0%
TTTGGTTAAGGGGACTTGGG+AGG + chr8_4:47973251-47973270 None:intergenic 50.0%
! AATGATGGGGGATTTGGGGA+AGG - chr8_4:47974551-47974570 Msa1329390:intron 50.0%
!! GGGGAAGGTATTTGAACGAC+AGG - chr8_4:47974566-47974585 Msa1329390:intron 50.0%
AACGACAGGGACTACTTTGG+AGG - chr8_4:47974580-47974599 Msa1329390:intron 50.0%
! GGATTTGCTCCAATGGCAGT+TGG - chr8_4:47974670-47974689 Msa1329390:intron 50.0%
TTTGCTCCAATGGCAGTTGG+AGG - chr8_4:47974673-47974692 Msa1329390:intron 50.0%
TTGCTCCAATGGCAGTTGGA+GGG - chr8_4:47974674-47974693 Msa1329390:intron 50.0%
AACACGCTTAGGGCTATGTC+TGG + chr8_4:47974754-47974773 None:intergenic 50.0%
ACTCGCTACCAACATGTTGG+TGG + chr8_4:47974891-47974910 None:intergenic 50.0%
TGGACTCGCTACCAACATGT+TGG + chr8_4:47974894-47974913 None:intergenic 50.0%
TGTCGACATAACCTCCACGA+TGG + chr8_4:47974914-47974933 None:intergenic 50.0%
GTGGAGGTTATGTCGACAGA+GGG - chr8_4:47974916-47974935 Msa1329390:CDS 50.0%
TTTGCCGCAATTAAGCTCGC+CGG - chr8_4:47974942-47974961 Msa1329390:CDS 50.0%
ATGACTAAGGGAGATGGGGT+TGG - chr8_4:47975132-47975151 Msa1329390:five_prime_UTR 50.0%
TCCTTGTAATGTGGGGATGG+AGG - chr8_4:47975288-47975307 Msa1329390:five_prime_UTR 50.0%
TTGGTTAAGGGGACTTGGGA+GGG + chr8_4:47973250-47973269 None:intergenic 50.0%
TTTGGTTAAGGGGACTTGGG+AGG + chr8_4:47973251-47973270 None:intergenic 50.0%
! AATGATGGGGGATTTGGGGA+AGG - chr8_4:47974551-47974570 Msa1329390:intron 50.0%
!! GGGGAAGGTATTTGAACGAC+AGG - chr8_4:47974566-47974585 Msa1329390:intron 50.0%
AACGACAGGGACTACTTTGG+AGG - chr8_4:47974580-47974599 Msa1329390:intron 50.0%
! GGATTTGCTCCAATGGCAGT+TGG - chr8_4:47974670-47974689 Msa1329390:intron 50.0%
TTTGCTCCAATGGCAGTTGG+AGG - chr8_4:47974673-47974692 Msa1329390:intron 50.0%
TTGCTCCAATGGCAGTTGGA+GGG - chr8_4:47974674-47974693 Msa1329390:intron 50.0%
AACACGCTTAGGGCTATGTC+TGG + chr8_4:47974754-47974773 None:intergenic 50.0%
ACTCGCTACCAACATGTTGG+TGG + chr8_4:47974891-47974910 None:intergenic 50.0%
TGGACTCGCTACCAACATGT+TGG + chr8_4:47974894-47974913 None:intergenic 50.0%
TGTCGACATAACCTCCACGA+TGG + chr8_4:47974914-47974933 None:intergenic 50.0%
GTGGAGGTTATGTCGACAGA+GGG - chr8_4:47974916-47974935 Msa1329390:CDS 50.0%
TTTGCCGCAATTAAGCTCGC+CGG - chr8_4:47974942-47974961 Msa1329390:CDS 50.0%
ATGACTAAGGGAGATGGGGT+TGG - chr8_4:47975132-47975151 Msa1329390:five_prime_UTR 50.0%
TCCTTGTAATGTGGGGATGG+AGG - chr8_4:47975288-47975307 Msa1329390:five_prime_UTR 50.0%
TGCTCCAATGGCAGTTGGAG+GGG - chr8_4:47974675-47974694 Msa1329390:intron 55.0%
ATGTCCCCTCCAACTGCCAT+TGG + chr8_4:47974682-47974701 None:intergenic 55.0%
AATGGCAGTTGGAGGGGACA+TGG - chr8_4:47974681-47974700 Msa1329390:intron 55.0%
AGATATTCCGCGACCACGAC+TGG + chr8_4:47974868-47974887 None:intergenic 55.0%
!! TGTTGGTAGCGAGTCCATCG+TGG - chr8_4:47974897-47974916 Msa1329390:CDS 55.0%
CGTGGAGGTTATGTCGACAG+AGG - chr8_4:47974915-47974934 Msa1329390:CDS 55.0%
ACGTCCGGCGAGCTTAATTG+CGG + chr8_4:47974949-47974968 None:intergenic 55.0%
! CTCGCCGGACGTTGTTATTC+CGG - chr8_4:47974957-47974976 Msa1329390:CDS 55.0%
GCCTCCATCCCCACATTACA+AGG + chr8_4:47975292-47975311 None:intergenic 55.0%
TGCTCCAATGGCAGTTGGAG+GGG - chr8_4:47974675-47974694 Msa1329390:intron 55.0%
ATGTCCCCTCCAACTGCCAT+TGG + chr8_4:47974682-47974701 None:intergenic 55.0%
AATGGCAGTTGGAGGGGACA+TGG - chr8_4:47974681-47974700 Msa1329390:intron 55.0%
AGATATTCCGCGACCACGAC+TGG + chr8_4:47974868-47974887 None:intergenic 55.0%
!! TGTTGGTAGCGAGTCCATCG+TGG - chr8_4:47974897-47974916 Msa1329390:CDS 55.0%
CGTGGAGGTTATGTCGACAG+AGG - chr8_4:47974915-47974934 Msa1329390:CDS 55.0%
ACGTCCGGCGAGCTTAATTG+CGG + chr8_4:47974949-47974968 None:intergenic 55.0%
! CTCGCCGGACGTTGTTATTC+CGG - chr8_4:47974957-47974976 Msa1329390:CDS 55.0%
GCCTCCATCCCCACATTACA+AGG + chr8_4:47975292-47975311 None:intergenic 55.0%
GTTAAGGGGACTTGGGAGGG+TGG + chr8_4:47973247-47973266 None:intergenic 60.0%
GGATCTAGCGGTTCCAGTCG+TGG - chr8_4:47974852-47974871 Msa1329390:CDS 60.0%
! TGGTAGCGAGTCCATCGTGG+AGG - chr8_4:47974900-47974919 Msa1329390:CDS 60.0%
GTTAAGGGGACTTGGGAGGG+TGG + chr8_4:47973247-47973266 None:intergenic 60.0%
GGATCTAGCGGTTCCAGTCG+TGG - chr8_4:47974852-47974871 Msa1329390:CDS 60.0%
! TGGTAGCGAGTCCATCGTGG+AGG - chr8_4:47974900-47974919 Msa1329390:CDS 60.0%
AGCGGTTCCAGTCGTGGTCG+CGG - chr8_4:47974858-47974877 Msa1329390:CDS 65.0%
AGCGGTTCCAGTCGTGGTCG+CGG - chr8_4:47974858-47974877 Msa1329390:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 47973146 47975313 47973146 ID=Msa1329390;Name=Msa1329390
chr8_4 mRNA 47973146 47975313 47973146 ID=Msa1329390-mRNA-1;Parent=Msa1329390;Name=Msa1329390-mRNA-1;_AED=0.00;_eAED=0.00;_QI=194|1|1|1|0|0.5|2|296|270
chr8_4 exon 47973146 47973886 47973146 ID=Msa1329390-mRNA-1:exon:13732;Parent=Msa1329390-mRNA-1
chr8_4 exon 47974752 47975313 47974752 ID=Msa1329390-mRNA-1:exon:13731;Parent=Msa1329390-mRNA-1
chr8_4 five_prime_UTR 47975120 47975313 47975120 ID=Msa1329390-mRNA-1:five_prime_utr;Parent=Msa1329390-mRNA-1
chr8_4 CDS 47974752 47975119 47974752 ID=Msa1329390-mRNA-1:cds;Parent=Msa1329390-mRNA-1
chr8_4 CDS 47973442 47973886 47973442 ID=Msa1329390-mRNA-1:cds;Parent=Msa1329390-mRNA-1
chr8_4 three_prime_UTR 47973146 47973441 47973146 ID=Msa1329390-mRNA-1:three_prime_utr;Parent=Msa1329390-mRNA-1
Gene Sequence

>Msa1329390

ATGGGGATAGTAGAAGAAGCTCACAACGTGAAAGTTTTGGGTTCAGGGTCACGGTTCATAGTTCTAGCTCACGGGTTTGGCACAGATCAATCTGTATGGAAACACCTCGTACCTCATCTCTTAGATGAGTTTCGTGTCATACTTTATGACAACATGGGAGCTGGTACAACTAATCCAGATTACTTTGATTTTGAGCGTTATTCAACCTTAGAAGGTTATGCTTATGATTTACTTGCAATCTTAGAAGAACTTCGAGTTGATTCATGTATCTTTGTTGGTCATTCTGTTTCTGCTATGATTGGAACTGTTGCATCAATTTCTCGACCTGATCTATTCAACAAAATTATTTTGATATCAGCTTCTCCAAGGTATTTGAACGACAGGGACTACTTTGGAGGATTTGAGCAAGAAGATTTGGATCAACTATTCGATGCTATGGCATCAAATTACAAATCATGGTGTTCTGGATTTGCTCCAATGGCAGTTGGAGGGGACATGGAGTCAGTAGCAGTACAAGAATTCAGTCGAACTTTGTTCAACATGAGACCAGACATAGCCCTAAGCGTGTTACAAACAATTTTTAAAAGTGACATGAGACAAATATTATGCATGGTCACTGTCCCATGCCACATTATACAAAGTATGAAGGATCTAGCGGTTCCAGTCGTGGTCGCGGAATATCTCCACCAACATGTTGGTAGCGAGTCCATCGTGGAGGTTATGTCGACAGAGGGTCATTTGCCGCAATTAAGCTCGCCGGACGTTGTTATTCCGGTGATACTCAAACACATTCGTCATGATATTGCGGCTTGA
Protein sequence

>Msa1329390

MGIVEEAHNVKVLGSGSRFIVLAHGFGTDQSVWKHLVPHLLDEFRVILYDNMGAGTTNPDYFDFERYSTLEGYAYDLLAILEELRVDSCIFVGHSVSAMIGTVASISRPDLFNKIILISASPRYLNDRDYFGGFEQEDLDQLFDAMASNYKSWCSGFAPMAVGGDMESVAVQEFSRTLFNMRPDIALSVLQTIFKSDMRQILCMVTVPCHIIQSMKDLAVPVVVAEYLHQHVGSESIVEVMSTEGHLPQLSSPDVVIPVILKHIRHDIAA*