AlfalfaGEDB | Msa1331830

M. sativa cultivar zhongmu-4 / Msa1331830

KEGG: K15014
GO: -
PFAM: PF01733.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1331830	XP_003609351.1	98.995	398	4	0	1	398	1	398	0.0	803

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1331830	sp\|Q84XI3\|ENT8_ARATH	63.520	392	129	5	10	398	9	389	1.55e-177	502

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1331830	G7JE40	98.995	398	4	0	1	398	1	398	0.0	803

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0269970	Msa1331830	0.809083	2.254209e-50	-8.615850e-47
Msa0349210	Msa1331830	0.812962	3.256316e-51	-8.615850e-47
Msa0903310	Msa1331830	0.827597	1.432534e-54	-8.615850e-47
Msa1105640	Msa1331830	0.803402	3.546133e-49	-8.615850e-47
Msa1214860	Msa1331830	0.966442	6.319610e-126	-8.615850e-47
Msa1300830	Msa1331830	0.976126	3.120133e-141	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1331830	MtrunA17_Chr4g0063911	98.995	398	4	0	1	398	1	398	0.0	803
Msa1331830	MtrunA17_Chr4g0020331	49.239	394	186	4	15	398	26	415	2.37e-127	373
Msa1331830	MtrunA17_Chr3g0103741	45.700	407	202	7	7	398	21	423	5.21e-114	339
Msa1331830	MtrunA17_Chr4g0022871	27.660	423	263	7	6	398	1	410	6.19e-49	171
Msa1331830	MtrunA17_Chr4g0022861	28.814	413	237	10	23	398	2	394	1.49e-46	164
Msa1331830	MtrunA17_Chr2g0323381	29.882	425	251	12	6	398	1	410	3.59e-46	163
Msa1331830	MtrunA17_Chr2g0280971	28.431	408	250	9	21	398	44	439	3.77e-46	164
Msa1331830	MtrunA17_Chr2g0280991	28.671	429	250	12	7	398	10	419	1.23e-45	162
Msa1331830	MtrunA17_Chr4g0022801	32.014	278	142	8	156	398	136	401	1.38e-26	110
Msa1331830	MtrunA17_Chr1g0184961	28.378	222	136	3	81	279	131	352	6.19e-23	99.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1331830	AT1G02630.1	63.520	392	129	5	10	398	9	389	1.58e-178	502
Msa1331830	AT1G02630.2	55.867	392	115	6	10	398	9	345	9.80e-146	417
Msa1331830	AT1G70330.1	49.114	395	186	4	16	398	59	450	4.08e-123	364
Msa1331830	AT3G09990.1	28.193	415	242	12	21	398	18	413	8.08e-44	157
Msa1331830	AT1G61630.1	27.378	431	249	9	9	398	5	412	5.95e-43	155
Msa1331830	AT4G05120.1	28.271	428	258	11	3	398	2	412	1.87e-42	154
Msa1331830	AT4G05120.2	28.271	428	258	11	3	398	2	412	1.87e-42	154
Msa1331830	AT4G05110.3	28.162	419	254	11	11	398	10	412	1.36e-41	152
Msa1331830	AT4G05110.1	28.162	419	254	11	11	398	10	412	1.36e-41	152
Msa1331830	AT4G05110.2	28.162	419	254	11	11	398	10	412	2.78e-41	151

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Find 106 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTAAGGGACCAGCTTT+TGG	0.257914	8_4:+51502234	Msa1331830:CDS
AAACTTTGTCCCTGAACATA+AGG	0.390189	8_4:-51502196	None:intergenic
AACACTAGTAAGATATCCAT+TGG	0.535884	8_4:-51502553	None:intergenic
AACTTTGTCCCTGAACATAA+GGG	0.516896	8_4:-51502195	None:intergenic
AAGTGCTCACTTGTACTTCA+TGG	0.413740	8_4:+51502074	Msa1331830:CDS
AATAGAAATAATATACTGTT+GGG	0.467486	8_4:+51502919	Msa1331830:three_prime_UTR
AATCCAAGGCTCTTAAGGAT+TGG	0.505326	8_4:+51502319	Msa1331830:CDS
AATGTAATGAATCTATAAAC+TGG	0.326934	8_4:+51502873	Msa1331830:three_prime_UTR
AATGTTTGTTATGTCTCTCA+TGG	0.465994	8_4:+51500578	Msa1331830:CDS
ACAGCTTGCATATACTGTTT+TGG	0.330078	8_4:-51500750	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCTACTTATGGTGATAAAG+TGG	-	chr8_4:51500115-51500134	None:intergenic	30.0%
TACATAATCCATTTCTTGCT+TGG	+	chr8_4:51500360-51500379	Msa1331830:CDS	30.0%
CCTTATTCGAACAAATTCTA+AGG	+	chr8_4:51501038-51501057	Msa1331830:intron	30.0%
AAAAACTTTGCCTTAGATGA+AGG	+	chr8_4:51501067-51501086	Msa1331830:intron	30.0%
TTTCATGTATCCTTCATCTA+AGG	-	chr8_4:51501080-51501099	None:intergenic	30.0%
ATACAAGCATTTGAAGGTTA+AGG	+	chr8_4:51501150-51501169	Msa1331830:intron	30.0%
TACAAGCATTTGAAGGTTAA+GGG	+	chr8_4:51501151-51501170	Msa1331830:intron	30.0%
ATGTTCCACAATGTATCAAA+AGG	+	chr8_4:51502409-51502428	Msa1331830:CDS	30.0%
TGTTCCACAATGTATCAAAA+GGG	+	chr8_4:51502410-51502429	Msa1331830:CDS	30.0%
ATTGTTCTTACATTTCTGCT+TGG	+	chr8_4:51502525-51502544	Msa1331830:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	51500110	51503025	51500110	ID=Msa1331830;Name=Msa1331830
chr8_4	mRNA	51500110	51503025	51500110	ID=Msa1331830-mRNA-1;Parent=Msa1331830;Name=Msa1331830-mRNA-1;_AED=0.01;_eAED=0.01;_QI=187\|1\|1\|1\|1\|1\|2\|324\|398
chr8_4	exon	51500110	51500795	51500110	ID=Msa1331830-mRNA-1:exon:15241;Parent=Msa1331830-mRNA-1
chr8_4	exon	51502004	51503025	51502004	ID=Msa1331830-mRNA-1:exon:15242;Parent=Msa1331830-mRNA-1
chr8_4	five_prime_UTR	51500110	51500296	51500110	ID=Msa1331830-mRNA-1:five_prime_utr;Parent=Msa1331830-mRNA-1
chr8_4	CDS	51500297	51500795	51500297	ID=Msa1331830-mRNA-1:cds;Parent=Msa1331830-mRNA-1
chr8_4	CDS	51502004	51502701	51502004	ID=Msa1331830-mRNA-1:cds;Parent=Msa1331830-mRNA-1
chr8_4	three_prime_UTR	51502702	51503025	51502702	ID=Msa1331830-mRNA-1:three_prime_utr;Parent=Msa1331830-mRNA-1

Gene Sequence

>Msa1331830

ATGGAAGCCGTTAAGCTAGCATCAAGTGATCCGAGCGAAACAAGAGACGCTTACAAAGTTGCTTACATAATCCATTTCTTGCTTGGAGCAGGAAACTTGCTACCTTGGAATGCATTTATCACGGCAGTTGATTACTTCGCGTACTTATATCCAACTAACCACATTGAGAAGGTTTTCGCTGTGGCTTATATGGTTTCATCTGTGCTTGTACTTCTTGCGATGATGAGTTGGGGAGGTTGGAGTAAAACAACACTGAGGTTGCGAATGAACTTAGGATTTTCAATGTTTGTTATGTCTCTCATGGTAGCTCCTGTCATTGATTGGGCCTCGAGTAGGGACGAGATGAAAGAGAGGCCAAGTGGTGCTTATGGCATGACAGTTGCAGCTGTTGTAATATGTGGTTTAGCAGATGGCTTGGTTGGTGGAAGTTTGATAGGATCAGCAGGAAGGCTTCCAAAACAGTATATGCAAGCTGTTTTTGCTGGAACTGCTTCCTCAGGTATTATAATTTCAATCTTGAGGATAATAACCAAAGCATCACTACCTCAAACCCCAAAGGGTCTCAAAATAAGTGCTCACTTGTACTTCATGGTGGCCATAGTTTTCCTCCTATGTTGCATTGTCTTTAGCAACTTGCAGCACAAGTTACCTATCATGCAGCAGTATCATCAGAGTGTACTTCAGGAGAGTCCCTTATGTTCAGGGACAAAGTTTTGTGCAGTGGCAGGGAAAATTAAGGGACCAGCTTTTGGAATATTCATAATCTATATAGTAACTCTATCTATCTTCCCAGGATTTATTGCTGAAGATCTTGAATCCAAGGCTCTTAAGGATTGGTATCCTATTATATTGATTACAGTTTATAATTTAGCTGATCTTATGGGGAAATCTTTAACTGCATTCTATGTTCCACAATGTATCAAAAGGGCCATAGGTGCTGCCACAGCAAGACTATTATTCTATCCACTTTTTATAGTTTGTCTTCATGGACCAAATTGGTTGAAAACAGAAGTGCCAATGATTGTTCTTACATTTCTGCTTGGATTTACCAATGGATATCTTACTAGTGTTCTTATGATTCTAACTCCTAAGTCAGTGCATTTCTCAGAATCAGAGTTATCTGCTATTGTGATGACCGCGTTCCTCGGATTCGGCTTGGTTGGTGGTTCTGTCCTAGGTTGGTTCTGGATACTGTGA

Protein sequence

>Msa1331830

MEAVKLASSDPSETRDAYKVAYIIHFLLGAGNLLPWNAFITAVDYFAYLYPTNHIEKVFAVAYMVSSVLVLLAMMSWGGWSKTTLRLRMNLGFSMFVMSLMVAPVIDWASSRDEMKERPSGAYGMTVAAVVICGLADGLVGGSLIGSAGRLPKQYMQAVFAGTASSGIIISILRIITKASLPQTPKGLKISAHLYFMVAIVFLLCCIVFSNLQHKLPIMQQYHQSVLQESPLCSGTKFCAVAGKIKGPAFGIFIIYIVTLSIFPGFIAEDLESKALKDWYPIILITVYNLADLMGKSLTAFYVPQCIKRAIGAATARLLFYPLFIVCLHGPNWLKTEVPMIVLTFLLGFTNGYLTSVLMILTPKSVHFSESELSAIVMTAFLGFGLVGGSVLGWFWIL*