AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1331830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1331830 MtrunA17_Chr4g0063911 98.995 398 4 0 1 398 1 398 0.0 803
Msa1331830 MtrunA17_Chr4g0020331 49.239 394 186 4 15 398 26 415 2.37e-127 373
Msa1331830 MtrunA17_Chr3g0103741 45.700 407 202 7 7 398 21 423 5.21e-114 339
Msa1331830 MtrunA17_Chr4g0022871 27.660 423 263 7 6 398 1 410 6.19e-49 171
Msa1331830 MtrunA17_Chr4g0022861 28.814 413 237 10 23 398 2 394 1.49e-46 164
Msa1331830 MtrunA17_Chr2g0323381 29.882 425 251 12 6 398 1 410 3.59e-46 163
Msa1331830 MtrunA17_Chr2g0280971 28.431 408 250 9 21 398 44 439 3.77e-46 164
Msa1331830 MtrunA17_Chr2g0280991 28.671 429 250 12 7 398 10 419 1.23e-45 162
Msa1331830 MtrunA17_Chr4g0022801 32.014 278 142 8 156 398 136 401 1.38e-26 110
Msa1331830 MtrunA17_Chr1g0184961 28.378 222 136 3 81 279 131 352 6.19e-23 99.0
Msa1331830 MtrunA17_Chr8g0335821 26.075 372 230 10 57 396 2 360 2.34e-22 97.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1331830 AT1G02630.1 63.520 392 129 5 10 398 9 389 1.58e-178 502
Msa1331830 AT1G02630.2 55.867 392 115 6 10 398 9 345 9.80e-146 417
Msa1331830 AT1G70330.1 49.114 395 186 4 16 398 59 450 4.08e-123 364
Msa1331830 AT3G09990.1 28.193 415 242 12 21 398 18 413 8.08e-44 157
Msa1331830 AT1G61630.1 27.378 431 249 9 9 398 5 412 5.95e-43 155
Msa1331830 AT4G05120.1 28.271 428 258 11 3 398 2 412 1.87e-42 154
Msa1331830 AT4G05120.2 28.271 428 258 11 3 398 2 412 1.87e-42 154
Msa1331830 AT4G05110.3 28.162 419 254 11 11 398 10 412 1.36e-41 152
Msa1331830 AT4G05110.1 28.162 419 254 11 11 398 10 412 1.36e-41 152
Msa1331830 AT4G05110.2 28.162 419 254 11 11 398 10 412 2.78e-41 151
Msa1331830 AT4G05130.1 27.791 421 253 12 11 398 10 412 1.76e-40 149
Msa1331830 AT4G05140.1 26.744 430 259 12 5 398 4 413 5.96e-38 142

Find 106 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTAAGGGACCAGCTTT+TGG 0.257914 8_4:+51502234 Msa1331830:CDS
TTATAATTTAGCTGATCTTA+TGG 0.261483 8_4:+51502365 Msa1331830:CDS
TGCAGTGGCAGGGAAAATTA+AGG 0.277433 8_4:+51502221 Msa1331830:CDS
GTTCTGTCCTAGGTTGGTTC+TGG 0.300139 8_4:+51502670 Msa1331830:CDS
TCTTGAATCCAAGGCTCTTA+AGG 0.316097 8_4:+51502314 Msa1331830:CDS
TAAACTGTAATCAATATAAT+AGG 0.319839 8_4:-51502345 None:intergenic
TGTCCACACTTCTCATGATT+TGG 0.326312 8_4:-51502812 None:intergenic
AATGTAATGAATCTATAAAC+TGG 0.326934 8_4:+51502873 Msa1331830:three_prime_UTR
ACAGCTTGCATATACTGTTT+TGG 0.330078 8_4:-51500750 None:intergenic
TATAATTTAGCTGATCTTAT+GGG 0.341304 8_4:+51502366 Msa1331830:CDS
TGTGAAGGCTTGTTTAGTTC+AGG 0.343097 8_4:+51502697 Msa1331830:exon
TACATAATCCATTTCTTGCT+TGG 0.351729 8_4:+51500360 Msa1331830:CDS
CAGGAGAGTCCCTTATGTTC+AGG 0.355351 8_4:+51502186 Msa1331830:CDS
GTATCATCAGAGTGTACTTC+AGG 0.361010 8_4:+51502167 Msa1331830:CDS
ATCACTTGATGCTAGCTTAA+CGG 0.372314 8_4:-51500304 None:intergenic
TGAGAAGTGTGGACATTGTT+AGG 0.373983 8_4:+51502820 Msa1331830:three_prime_UTR
TGGTAGCTCCTGTCATTGAT+TGG 0.376095 8_4:+51500598 Msa1331830:CDS
GAGAGGCCAAGTGGTGCTTA+TGG 0.382783 8_4:+51500645 Msa1331830:CDS
TTGGTTGGTGGTTCTGTCCT+AGG 0.383076 8_4:+51502660 Msa1331830:CDS
AAACTTTGTCCCTGAACATA+AGG 0.390189 8_4:-51502196 None:intergenic
TGATGATACTGCTGCATGAT+AGG 0.394711 8_4:-51502153 None:intergenic
TACAAGTAGAATTCTACTTA+TGG 0.398475 8_4:-51500123 None:intergenic
TTTGTCTTCATGGACCAAAT+TGG 0.398789 8_4:+51502481 Msa1331830:CDS
ACCTTCTCAATGTGGTTAGT+TGG 0.405196 8_4:-51500447 None:intergenic
TTCCTGCTCCAAGCAAGAAA+TGG 0.405518 8_4:-51500368 None:intergenic
CTCGGATTCGGCTTGGTTGG+TGG 0.408001 8_4:+51502648 Msa1331830:CDS
TTGAGACCCTTTGGGGTTTG+AGG 0.410939 8_4:-51502048 None:intergenic
TCTTGCTGTGGCAGCACCTA+TGG 0.413596 8_4:-51502433 None:intergenic
AAGTGCTCACTTGTACTTCA+TGG 0.413740 8_4:+51502074 Msa1331830:CDS
ATCCATTTCTTGCTTGGAGC+AGG 0.419667 8_4:+51500366 Msa1331830:CDS
TATCGCAAAAGGCAATCTCT+TGG 0.420101 8_4:+51502962 Msa1331830:three_prime_UTR
ATGACCGCGTTCCTCGGATT+CGG 0.421562 8_4:+51502636 Msa1331830:CDS
ATGTTCCACAATGTATCAAA+AGG 0.427242 8_4:+51502409 Msa1331830:CDS
TTGGTTGGTGGAAGTTTGAT+AGG 0.433123 8_4:+51500711 Msa1331830:CDS
ACTTCTTGCGATGATGAGTT+GGG 0.436575 8_4:+51500506 Msa1331830:CDS
TTTGCTGGAACTGCTTCCTC+AGG 0.440158 8_4:+51500774 Msa1331830:CDS
ATACCAATCCTTAAGAGCCT+TGG 0.444568 8_4:-51502322 None:intergenic
GAGCAGGAAACTTGCTACCT+TGG 0.445287 8_4:+51500382 Msa1331830:CDS
TACTTCTTGCGATGATGAGT+TGG 0.445586 8_4:+51500505 Msa1331830:CDS
AGATCTTCAGCAATAAATCC+TGG 0.450755 8_4:-51502294 None:intergenic
GGTAGCTCCTGTCATTGATT+GGG 0.452656 8_4:+51500599 Msa1331830:CDS
GCAACATAGGAGGAAAACTA+TGG 0.455079 8_4:-51502100 None:intergenic
ATTGTTCTTACATTTCTGCT+TGG 0.455315 8_4:+51502525 Msa1331830:CDS
ATTGTGATGACCGCGTTCCT+CGG 0.461564 8_4:+51502630 Msa1331830:CDS
AATGTTTGTTATGTCTCTCA+TGG 0.465994 8_4:+51500578 Msa1331830:CDS
AATAGAAATAATATACTGTT+GGG 0.467486 8_4:+51502919 Msa1331830:three_prime_UTR
CTGAGGTTGCGAATGAACTT+AGG 0.472940 8_4:+51500549 Msa1331830:CDS
GGTTTGAGGTAGTGATGCTT+TGG 0.473253 8_4:-51502034 None:intergenic
AGGAGAGTCCCTTATGTTCA+GGG 0.476690 8_4:+51502187 Msa1331830:CDS
CGATGATGAGTTGGGGAGGT+TGG 0.479957 8_4:+51500514 Msa1331830:CDS
CATTGATTGGGCCTCGAGTA+GGG 0.484656 8_4:+51500611 Msa1331830:CDS
TCTGAGAAATGCACTGACTT+AGG 0.485958 8_4:-51502591 None:intergenic
AGAAATGTAAGAACAATCAT+TGG 0.487588 8_4:-51502519 None:intergenic
TCATTGATTGGGCCTCGAGT+AGG 0.493347 8_4:+51500610 Msa1331830:CDS
CAATAGAAATAATATACTGT+TGG 0.499673 8_4:+51502918 Msa1331830:three_prime_UTR
TAAGCGTCTCTTGTTTCGCT+CGG 0.504224 8_4:-51500327 None:intergenic
TTCCTCGGATTCGGCTTGGT+TGG 0.504238 8_4:+51502645 Msa1331830:CDS
TGCTGAAGATCTTGAATCCA+AGG 0.504556 8_4:+51502305 Msa1331830:CDS
AATCCAAGGCTCTTAAGGAT+TGG 0.505326 8_4:+51502319 Msa1331830:CDS
ATTATGAATATTCCAAAAGC+TGG 0.509136 8_4:-51502246 None:intergenic
GTTGCAGCTGTTGTAATATG+TGG 0.509743 8_4:+51500675 Msa1331830:CDS
GGTTTAGCAGATGGCTTGGT+TGG 0.510374 8_4:+51500696 Msa1331830:CDS
GCAGTGGCAGGGAAAATTAA+GGG 0.512760 8_4:+51502222 Msa1331830:CDS
AACTTTGTCCCTGAACATAA+GGG 0.516896 8_4:-51502195 None:intergenic
GTATTATAATTTCAATCTTG+AGG 0.517953 8_4:+51502004 Msa1331830:CDS
GTTGGTTCTGGATACTGTGA+AGG 0.522252 8_4:+51502682 Msa1331830:CDS
TTGGTGGTTCTGTCCTAGGT+TGG 0.528954 8_4:+51502664 Msa1331830:CDS
TTAGCAGATGGCTTGGTTGG+TGG 0.535563 8_4:+51500699 Msa1331830:CDS
TTCTACTTATGGTGATAAAG+TGG 0.535803 8_4:-51500112 None:intergenic
AACACTAGTAAGATATCCAT+TGG 0.535884 8_4:-51502553 None:intergenic
TCACTACCTCAAACCCCAAA+GGG 0.535947 8_4:+51502042 Msa1331830:CDS
TTTCTGCTTGGATTTACCAA+TGG 0.538009 8_4:+51502537 Msa1331830:CDS
GGAAGTTTGATAGGATCAGC+AGG 0.544382 8_4:+51500720 Msa1331830:CDS
CAATGTATCAAAAGGGCCAT+AGG 0.548021 8_4:+51502417 Msa1331830:CDS
CTCGAGGCCCAATCAATGAC+AGG 0.549987 8_4:-51500606 None:intergenic
ATTTACTTCATTCAGAACTT+CGG 0.551328 8_4:+51500240 Msa1331830:five_prime_UTR
ATAATTTAGCTGATCTTATG+GGG 0.556528 8_4:+51502367 Msa1331830:CDS
TGTTCCACAATGTATCAAAA+GGG 0.559037 8_4:+51502410 Msa1331830:CDS
GTAATATGTGGTTTAGCAGA+TGG 0.566070 8_4:+51500687 Msa1331830:CDS
TGCTAAAGACAATGCAACAT+AGG 0.569551 8_4:-51502113 None:intergenic
GATCTTCAGCAATAAATCCT+GGG 0.574505 8_4:-51502293 None:intergenic
TACTCATAGAAGAGACGAGA+TGG 0.574855 8_4:+51502995 Msa1331830:three_prime_UTR
ATGTGGTTTAGCAGATGGCT+TGG 0.575031 8_4:+51500692 Msa1331830:CDS
GTAACTCTATCTATCTTCCC+AGG 0.575256 8_4:+51502276 Msa1331830:CDS
ACCTTGGAATGCATTTATCA+CGG 0.589722 8_4:+51500398 Msa1331830:CDS
TAAAGACAATGCAACATAGG+AGG 0.600160 8_4:-51502110 None:intergenic
CACCAACCAAGCCGAATCCG+AGG 0.600577 8_4:-51502647 None:intergenic
ATGTAATGAATCTATAAACT+GGG 0.601993 8_4:+51502874 Msa1331830:three_prime_UTR
TCCAACTAACCACATTGAGA+AGG 0.605730 8_4:+51500446 Msa1331830:CDS
ATCACTACCTCAAACCCCAA+AGG 0.611165 8_4:+51502041 Msa1331830:CDS
GTCATGCCATAAGCACCACT+TGG 0.614144 8_4:-51500651 None:intergenic
ATATAGAAACAATCTACTTG+AGG 0.614819 8_4:-51502767 None:intergenic
ACAGTATCCAGAACCAACCT+AGG 0.614984 8_4:-51502677 None:intergenic
CTTGCGATGATGAGTTGGGG+AGG 0.619198 8_4:+51500510 Msa1331830:CDS
TTTCATCTCGTCCCTACTCG+AGG 0.623295 8_4:-51500622 None:intergenic
GTTTGATAGGATCAGCAGGA+AGG 0.624638 8_4:+51500724 Msa1331830:CDS
GCCGTGATAAATGCATTCCA+AGG 0.626474 8_4:-51500399 None:intergenic
GTAGGGACGAGATGAAAGAG+AGG 0.629913 8_4:+51500628 Msa1331830:CDS
ATAGAATAATAGTCTTGCTG+TGG 0.649512 8_4:-51502445 None:intergenic
CTTCTTGCGATGATGAGTTG+GGG 0.656503 8_4:+51500507 Msa1331830:CDS
CAGCGAAAACCTTCTCAATG+TGG 0.674870 8_4:-51500455 None:intergenic
GTGCCAAATCATGAGAAGTG+TGG 0.697483 8_4:+51502809 Msa1331830:three_prime_UTR
TGCTCACTTGTACTTCATGG+TGG 0.701698 8_4:+51502077 Msa1331830:CDS
CAAGCCGAATCCGAGGAACG+CGG 0.703497 8_4:-51502640 None:intergenic
GAGATGAAAGAGAGGCCAAG+TGG 0.710667 8_4:+51500636 Msa1331830:CDS
GTTGGAGTAAAACAACACTG+AGG 0.825176 8_4:+51500532 Msa1331830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACAACATATATATTAAAAT+TGG + chr8_4:51501727-51501746 Msa1331830:intron 10.0%
!! ACAACATATATATTAAAATT+GGG + chr8_4:51501728-51501747 Msa1331830:intron 10.0%
!!! TAAAATTATTTTGAAGATGT+TGG - chr8_4:51500170-51500189 None:intergenic 15.0%
!! TATAAGAAAGTGAAAAAATA+TGG + chr8_4:51500278-51500297 Msa1331830:exon 15.0%
!!! TTTTAGATAGTAATGTAAAA+AGG - chr8_4:51500856-51500875 None:intergenic 15.0%
!!! TAAATTTTTTGTTACAATGA+CGG - chr8_4:51501370-51501389 None:intergenic 15.0%
!! TAAATCTATACCATAATTTA+TGG + chr8_4:51501639-51501658 Msa1331830:intron 15.0%
!!! TTTTCCAAATTTCATTTTAA+AGG + chr8_4:51501702-51501721 Msa1331830:intron 15.0%
!! ATCAAGACAATTAAATTAAA+AGG + chr8_4:51501791-51501810 Msa1331830:intron 15.0%
!! TAAACTGTAATCAATATAAT+AGG - chr8_4:51502348-51502367 None:intergenic 15.0%
!! AATAGAAATAATATACTGTT+GGG + chr8_4:51502919-51502938 Msa1331830:three_prime_UTR 15.0%
!!! TTTTCAAAAATAGAGTGAAA+GGG - chr8_4:51500217-51500236 None:intergenic 20.0%
!! ATTTAACAACATTTCTAACT+AGG - chr8_4:51500916-51500935 None:intergenic 20.0%
!! ATTCATGTATAAATTCAAGT+GGG - chr8_4:51501318-51501337 None:intergenic 20.0%
!! TATTCATGTATAAATTCAAG+TGG - chr8_4:51501319-51501338 None:intergenic 20.0%
!! AAATTAGTTGCCATAAATTA+TGG - chr8_4:51501652-51501671 None:intergenic 20.0%
!!! TATGCCTTTAAAATGAAATT+TGG - chr8_4:51501709-51501728 None:intergenic 20.0%
!!! CTCTAAAAAAAAATCTATTG+TGG - chr8_4:51501859-51501878 None:intergenic 20.0%
!!! ATATTAATAAAAGCCTTTTG+TGG + chr8_4:51501974-51501993 Msa1331830:intron 20.0%
!! GTATTATAATTTCAATCTTG+AGG + chr8_4:51502004-51502023 Msa1331830:CDS 20.0%
!! TTATAATTTAGCTGATCTTA+TGG + chr8_4:51502365-51502384 Msa1331830:CDS 20.0%
!! TATAATTTAGCTGATCTTAT+GGG + chr8_4:51502366-51502385 Msa1331830:CDS 20.0%
!! AATGTAATGAATCTATAAAC+TGG + chr8_4:51502873-51502892 Msa1331830:three_prime_UTR 20.0%
!! ATGTAATGAATCTATAAACT+GGG + chr8_4:51502874-51502893 Msa1331830:three_prime_UTR 20.0%
!! CAATAGAAATAATATACTGT+TGG + chr8_4:51502918-51502937 Msa1331830:three_prime_UTR 20.0%
! TACAAGTAGAATTCTACTTA+TGG - chr8_4:51500126-51500145 None:intergenic 25.0%
!! CTTTTCAAAAATAGAGTGAA+AGG - chr8_4:51500218-51500237 None:intergenic 25.0%
! ATTTACTTCATTCAGAACTT+CGG + chr8_4:51500240-51500259 Msa1331830:five_prime_UTR 25.0%
! GTAAAATTGATCAACTATGA+GGG - chr8_4:51500830-51500849 None:intergenic 25.0%
! AGTAAAATTGATCAACTATG+AGG - chr8_4:51500831-51500850 None:intergenic 25.0%
! ATAAAATACTCATTGTCGTA+TGG - chr8_4:51501118-51501137 None:intergenic 25.0%
! AAGTATATACAAGCATTTGA+AGG + chr8_4:51501144-51501163 Msa1331830:intron 25.0%
!!! GCATTTGTCTTTTTTTCTAA+AGG + chr8_4:51501230-51501249 Msa1331830:intron 25.0%
!!! TTGTCTTTTTTTCTAAAGGA+TGG + chr8_4:51501234-51501253 Msa1331830:intron 25.0%
!!! TGTCTTTTTTTCTAAAGGAT+GGG + chr8_4:51501235-51501254 Msa1331830:intron 25.0%
! ATTATGAATATTCCAAAAGC+TGG - chr8_4:51502249-51502268 None:intergenic 25.0%
! ATAATTTAGCTGATCTTATG+GGG + chr8_4:51502367-51502386 Msa1331830:CDS 25.0%
! TGAAGACAAACTATAAAAAG+TGG - chr8_4:51502471-51502490 None:intergenic 25.0%
!!! CTTTTTATAGTTTGTCTTCA+TGG + chr8_4:51502471-51502490 Msa1331830:CDS 25.0%
! AGAAATGTAAGAACAATCAT+TGG - chr8_4:51502522-51502541 None:intergenic 25.0%
! ATATAGAAACAATCTACTTG+AGG - chr8_4:51502770-51502789 None:intergenic 25.0%
TTCTACTTATGGTGATAAAG+TGG - chr8_4:51500115-51500134 None:intergenic 30.0%
TACATAATCCATTTCTTGCT+TGG + chr8_4:51500360-51500379 Msa1331830:CDS 30.0%
!! AATGTTTGTTATGTCTCTCA+TGG + chr8_4:51500578-51500597 Msa1331830:CDS 30.0%
! TTGTGTTGTAATTTTACCTG+AGG - chr8_4:51500793-51500812 None:intergenic 30.0%
! CTATCTAAAACAATGTCCTT+AGG + chr8_4:51500866-51500885 Msa1331830:intron 30.0%
! CCTTAGAATTTGTTCGAATA+AGG - chr8_4:51501041-51501060 None:intergenic 30.0%
CCTTATTCGAACAAATTCTA+AGG + chr8_4:51501038-51501057 Msa1331830:intron 30.0%
AAAAACTTTGCCTTAGATGA+AGG + chr8_4:51501067-51501086 Msa1331830:intron 30.0%
TTTCATGTATCCTTCATCTA+AGG - chr8_4:51501080-51501099 None:intergenic 30.0%
ATACAAGCATTTGAAGGTTA+AGG + chr8_4:51501150-51501169 Msa1331830:intron 30.0%
TACAAGCATTTGAAGGTTAA+GGG + chr8_4:51501151-51501170 Msa1331830:intron 30.0%
!!! GTCTTTTTTTCTAAAGGATG+GGG + chr8_4:51501236-51501255 Msa1331830:intron 30.0%
!!! TCTTTTTTTCTAAAGGATGG+GGG + chr8_4:51501237-51501256 Msa1331830:intron 30.0%
!! CATAGTTTTGCTAGATGTAT+AGG - chr8_4:51501452-51501471 None:intergenic 30.0%
!!! CTTAAAGAATTTTGAAGCTG+TGG + chr8_4:51501482-51501501 Msa1331830:intron 30.0%
! TTTGCTAGGTTATAGTTTGT+AGG + chr8_4:51501531-51501550 Msa1331830:intron 30.0%
ATGTTCCACAATGTATCAAA+AGG + chr8_4:51502409-51502428 Msa1331830:CDS 30.0%
TGTTCCACAATGTATCAAAA+GGG + chr8_4:51502410-51502429 Msa1331830:CDS 30.0%
! ATAGAATAATAGTCTTGCTG+TGG - chr8_4:51502448-51502467 None:intergenic 30.0%
! ACTTCTGTTTTCAACCAATT+TGG - chr8_4:51502498-51502517 None:intergenic 30.0%
ATTGTTCTTACATTTCTGCT+TGG + chr8_4:51502525-51502544 Msa1331830:CDS 30.0%
AACACTAGTAAGATATCCAT+TGG - chr8_4:51502556-51502575 None:intergenic 30.0%
! GACATTGTTAGGAAACTTTT+AGG + chr8_4:51502831-51502850 Msa1331830:three_prime_UTR 30.0%
! TTTTGCGATAGAAAACATGT+AGG - chr8_4:51502952-51502971 None:intergenic 30.0%
! TACATGTTTTCTATCGCAAA+AGG + chr8_4:51502951-51502970 Msa1331830:three_prime_UTR 30.0%
! ATCACTTGATGCTAGCTTAA+CGG - chr8_4:51500307-51500326 None:intergenic 35.0%
ACCTTGGAATGCATTTATCA+CGG + chr8_4:51500398-51500417 Msa1331830:CDS 35.0%
GTAATATGTGGTTTAGCAGA+TGG + chr8_4:51500687-51500706 Msa1331830:CDS 35.0%
! ACAGCTTGCATATACTGTTT+TGG - chr8_4:51500753-51500772 None:intergenic 35.0%
!!! TATATGCAAGCTGTTTTTGC+TGG + chr8_4:51500759-51500778 Msa1331830:CDS 35.0%
CTAAAACAATGTCCTTAGGT+AGG + chr8_4:51500870-51500889 Msa1331830:intron 35.0%
ATTGACAACACTGATACAAG+AGG + chr8_4:51501002-51501021 Msa1331830:intron 35.0%
TTGAAGGTTAAGGGAGAAAA+AGG + chr8_4:51501160-51501179 Msa1331830:intron 35.0%
GGAAAGTAGATTCTAGATCA+TGG + chr8_4:51501181-51501200 Msa1331830:intron 35.0%
! CTAGGTTATAGTTTGTAGGA+GGG + chr8_4:51501535-51501554 Msa1331830:intron 35.0%
TAGGAGGGACTAAAATTTGA+TGG + chr8_4:51501550-51501569 Msa1331830:intron 35.0%
!! TTGATGGCCTCTACTTATTT+TGG + chr8_4:51501566-51501585 Msa1331830:intron 35.0%
GATCTGACCAAAATAAGTAG+AGG - chr8_4:51501576-51501595 None:intergenic 35.0%
TATCTATCCGAACATACTAG+CGG - chr8_4:51501906-51501925 None:intergenic 35.0%
TAATACCTGTCAACCACAAA+AGG - chr8_4:51501990-51502009 None:intergenic 35.0%
!!! ACTTATTTTGAGACCCTTTG+GGG - chr8_4:51502058-51502077 None:intergenic 35.0%
!!! CACTTATTTTGAGACCCTTT+GGG - chr8_4:51502059-51502078 None:intergenic 35.0%
TAAAGACAATGCAACATAGG+AGG - chr8_4:51502113-51502132 None:intergenic 35.0%
TGCTAAAGACAATGCAACAT+AGG - chr8_4:51502116-51502135 None:intergenic 35.0%
AACTTTGTCCCTGAACATAA+GGG - chr8_4:51502198-51502217 None:intergenic 35.0%
AAACTTTGTCCCTGAACATA+AGG - chr8_4:51502199-51502218 None:intergenic 35.0%
! AAAATTAAGGGACCAGCTTT+TGG + chr8_4:51502234-51502253 Msa1331830:CDS 35.0%
GATCTTCAGCAATAAATCCT+GGG - chr8_4:51502296-51502315 None:intergenic 35.0%
AGATCTTCAGCAATAAATCC+TGG - chr8_4:51502297-51502316 None:intergenic 35.0%
TTTGTCTTCATGGACCAAAT+TGG + chr8_4:51502481-51502500 Msa1331830:CDS 35.0%
TTTCTGCTTGGATTTACCAA+TGG + chr8_4:51502537-51502556 Msa1331830:CDS 35.0%
TCCAACTAACCACATTGAGA+AGG + chr8_4:51500446-51500465 Msa1331830:CDS 40.0%
ACCTTCTCAATGTGGTTAGT+TGG - chr8_4:51500450-51500469 None:intergenic 40.0%
TACTTCTTGCGATGATGAGT+TGG + chr8_4:51500505-51500524 Msa1331830:CDS 40.0%
ACTTCTTGCGATGATGAGTT+GGG + chr8_4:51500506-51500525 Msa1331830:CDS 40.0%
GTTGGAGTAAAACAACACTG+AGG + chr8_4:51500532-51500551 Msa1331830:CDS 40.0%
GTTGCAGCTGTTGTAATATG+TGG + chr8_4:51500675-51500694 Msa1331830:CDS 40.0%
!! TTGGTTGGTGGAAGTTTGAT+AGG + chr8_4:51500711-51500730 Msa1331830:CDS 40.0%
AGATCCACAGCTACCATTAT+GGG + chr8_4:51501206-51501225 Msa1331830:intron 40.0%
AAAGACAAATGCCCCCATAA+TGG - chr8_4:51501222-51501241 None:intergenic 40.0%
!! ACTGCTTTTCTTGCTTTGCT+AGG + chr8_4:51501517-51501536 Msa1331830:intron 40.0%
! GCTAGGTTATAGTTTGTAGG+AGG + chr8_4:51501534-51501553 Msa1331830:intron 40.0%
CATCTTTCCGCTAGTATGTT+CGG + chr8_4:51501896-51501915 Msa1331830:intron 40.0%
! AAAAGCCTTTTGTGGTTGAC+AGG + chr8_4:51501982-51502001 Msa1331830:intron 40.0%
!!! GCACTTATTTTGAGACCCTT+TGG - chr8_4:51502060-51502079 None:intergenic 40.0%
AAGTGCTCACTTGTACTTCA+TGG + chr8_4:51502074-51502093 Msa1331830:CDS 40.0%
GCAACATAGGAGGAAAACTA+TGG - chr8_4:51502103-51502122 None:intergenic 40.0%
TGATGATACTGCTGCATGAT+AGG - chr8_4:51502156-51502175 None:intergenic 40.0%
GTATCATCAGAGTGTACTTC+AGG + chr8_4:51502167-51502186 Msa1331830:CDS 40.0%
GTAACTCTATCTATCTTCCC+AGG + chr8_4:51502276-51502295 Msa1331830:CDS 40.0%
TGCTGAAGATCTTGAATCCA+AGG + chr8_4:51502305-51502324 Msa1331830:CDS 40.0%
TCTTGAATCCAAGGCTCTTA+AGG + chr8_4:51502314-51502333 Msa1331830:CDS 40.0%
AATCCAAGGCTCTTAAGGAT+TGG + chr8_4:51502319-51502338 Msa1331830:CDS 40.0%
ATACCAATCCTTAAGAGCCT+TGG - chr8_4:51502325-51502344 None:intergenic 40.0%
! ATGGCCCTTTTGATACATTG+TGG - chr8_4:51502417-51502436 None:intergenic 40.0%
CAATGTATCAAAAGGGCCAT+AGG + chr8_4:51502417-51502436 Msa1331830:CDS 40.0%
!! TCTGAGAAATGCACTGACTT+AGG - chr8_4:51502594-51502613 None:intergenic 40.0%
! TGTGAAGGCTTGTTTAGTTC+AGG + chr8_4:51502697-51502716 Msa1331830:exon 40.0%
TGTCCACACTTCTCATGATT+TGG - chr8_4:51502815-51502834 None:intergenic 40.0%
! TGAGAAGTGTGGACATTGTT+AGG + chr8_4:51502820-51502839 Msa1331830:three_prime_UTR 40.0%
TATCGCAAAAGGCAATCTCT+TGG + chr8_4:51502962-51502981 Msa1331830:three_prime_UTR 40.0%
TACTCATAGAAGAGACGAGA+TGG + chr8_4:51502995-51503014 Msa1331830:three_prime_UTR 40.0%
! TAAGCGTCTCTTGTTTCGCT+CGG - chr8_4:51500330-51500349 None:intergenic 45.0%
ATCCATTTCTTGCTTGGAGC+AGG + chr8_4:51500366-51500385 Msa1331830:CDS 45.0%
TTCCTGCTCCAAGCAAGAAA+TGG - chr8_4:51500371-51500390 None:intergenic 45.0%
GCCGTGATAAATGCATTCCA+AGG - chr8_4:51500402-51500421 None:intergenic 45.0%
CAGCGAAAACCTTCTCAATG+TGG - chr8_4:51500458-51500477 None:intergenic 45.0%
! CATTGAGAAGGTTTTCGCTG+TGG + chr8_4:51500458-51500477 Msa1331830:CDS 45.0%
! GGTTTTCGCTGTGGCTTATA+TGG + chr8_4:51500467-51500486 Msa1331830:CDS 45.0%
CTTCTTGCGATGATGAGTTG+GGG + chr8_4:51500507-51500526 Msa1331830:CDS 45.0%
CTGAGGTTGCGAATGAACTT+AGG + chr8_4:51500549-51500568 Msa1331830:CDS 45.0%
TGGTAGCTCCTGTCATTGAT+TGG + chr8_4:51500598-51500617 Msa1331830:CDS 45.0%
! GGTAGCTCCTGTCATTGATT+GGG + chr8_4:51500599-51500618 Msa1331830:CDS 45.0%
ATGTGGTTTAGCAGATGGCT+TGG + chr8_4:51500692-51500711 Msa1331830:CDS 45.0%
GGAAGTTTGATAGGATCAGC+AGG + chr8_4:51500720-51500739 Msa1331830:CDS 45.0%
GTTTGATAGGATCAGCAGGA+AGG + chr8_4:51500724-51500743 Msa1331830:CDS 45.0%
CAGATCCACAGCTACCATTA+TGG + chr8_4:51501205-51501224 Msa1331830:intron 45.0%
GATCCACAGCTACCATTATG+GGG + chr8_4:51501207-51501226 Msa1331830:intron 45.0%
ATCCACAGCTACCATTATGG+GGG + chr8_4:51501208-51501227 Msa1331830:intron 45.0%
! GGTTTGAGGTAGTGATGCTT+TGG - chr8_4:51502037-51502056 None:intergenic 45.0%
ATCACTACCTCAAACCCCAA+AGG + chr8_4:51502041-51502060 Msa1331830:CDS 45.0%
TCACTACCTCAAACCCCAAA+GGG + chr8_4:51502042-51502061 Msa1331830:CDS 45.0%
TGCTCACTTGTACTTCATGG+TGG + chr8_4:51502077-51502096 Msa1331830:CDS 45.0%
AGGAGAGTCCCTTATGTTCA+GGG + chr8_4:51502187-51502206 Msa1331830:CDS 45.0%
!! AGGGACAAAGTTTTGTGCAG+TGG + chr8_4:51502206-51502225 Msa1331830:CDS 45.0%
!! ACAAAGTTTTGTGCAGTGGC+AGG + chr8_4:51502210-51502229 Msa1331830:CDS 45.0%
!! CAAAGTTTTGTGCAGTGGCA+GGG + chr8_4:51502211-51502230 Msa1331830:CDS 45.0%
TGCAGTGGCAGGGAAAATTA+AGG + chr8_4:51502221-51502240 Msa1331830:CDS 45.0%
GCAGTGGCAGGGAAAATTAA+GGG + chr8_4:51502222-51502241 Msa1331830:CDS 45.0%
ACAGTATCCAGAACCAACCT+AGG - chr8_4:51502680-51502699 None:intergenic 45.0%
!! GTTGGTTCTGGATACTGTGA+AGG + chr8_4:51502682-51502701 Msa1331830:CDS 45.0%
GTGCCAAATCATGAGAAGTG+TGG + chr8_4:51502809-51502828 Msa1331830:three_prime_UTR 45.0%
!! GAGCAGGAAACTTGCTACCT+TGG + chr8_4:51500382-51500401 Msa1331830:CDS 50.0%
! TCATTGATTGGGCCTCGAGT+AGG + chr8_4:51500610-51500629 Msa1331830:CDS 50.0%
! CATTGATTGGGCCTCGAGTA+GGG + chr8_4:51500611-51500630 Msa1331830:CDS 50.0%
TTTCATCTCGTCCCTACTCG+AGG - chr8_4:51500625-51500644 None:intergenic 50.0%
GTAGGGACGAGATGAAAGAG+AGG + chr8_4:51500628-51500647 Msa1331830:CDS 50.0%
GAGATGAAAGAGAGGCCAAG+TGG + chr8_4:51500636-51500655 Msa1331830:CDS 50.0%
GTCATGCCATAAGCACCACT+TGG - chr8_4:51500654-51500673 None:intergenic 50.0%
!! GGTTTAGCAGATGGCTTGGT+TGG + chr8_4:51500696-51500715 Msa1331830:CDS 50.0%
!! TTAGCAGATGGCTTGGTTGG+TGG + chr8_4:51500699-51500718 Msa1331830:CDS 50.0%
TTTGCTGGAACTGCTTCCTC+AGG + chr8_4:51500774-51500793 Msa1331830:CDS 50.0%
!! TTGAGACCCTTTGGGGTTTG+AGG - chr8_4:51502051-51502070 None:intergenic 50.0%
CAGGAGAGTCCCTTATGTTC+AGG + chr8_4:51502186-51502205 Msa1331830:CDS 50.0%
ATTGTGATGACCGCGTTCCT+CGG + chr8_4:51502630-51502649 Msa1331830:CDS 50.0%
!! TTGGTTGGTGGTTCTGTCCT+AGG + chr8_4:51502660-51502679 Msa1331830:CDS 50.0%
! TTGGTGGTTCTGTCCTAGGT+TGG + chr8_4:51502664-51502683 Msa1331830:CDS 50.0%
!! GTTCTGTCCTAGGTTGGTTC+TGG + chr8_4:51502670-51502689 Msa1331830:CDS 50.0%
! CTTGCGATGATGAGTTGGGG+AGG + chr8_4:51500510-51500529 Msa1331830:CDS 55.0%
! CGATGATGAGTTGGGGAGGT+TGG + chr8_4:51500514-51500533 Msa1331830:CDS 55.0%
CTCGAGGCCCAATCAATGAC+AGG - chr8_4:51500609-51500628 None:intergenic 55.0%
GAGAGGCCAAGTGGTGCTTA+TGG + chr8_4:51500645-51500664 Msa1331830:CDS 55.0%
CGCGCTATAGCTCCTACCTA+AGG - chr8_4:51500885-51500904 None:intergenic 55.0%
TGCCCCCATAATGGTAGCTG+TGG - chr8_4:51501213-51501232 None:intergenic 55.0%
! TCTTGCTGTGGCAGCACCTA+TGG - chr8_4:51502436-51502455 None:intergenic 55.0%
ATGACCGCGTTCCTCGGATT+CGG + chr8_4:51502636-51502655 Msa1331830:CDS 55.0%
!! TTCCTCGGATTCGGCTTGGT+TGG + chr8_4:51502645-51502664 Msa1331830:CDS 55.0%
CAAGCCGAATCCGAGGAACG+CGG - chr8_4:51502643-51502662 None:intergenic 60.0%
CACCAACCAAGCCGAATCCG+AGG - chr8_4:51502650-51502669 None:intergenic 60.0%
!! CTCGGATTCGGCTTGGTTGG+TGG + chr8_4:51502648-51502667 Msa1331830:CDS 60.0%
CGCGTTCCTCGGATTCGGCT+TGG + chr8_4:51502641-51502660 Msa1331830:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 51500110 51503025 51500110 ID=Msa1331830;Name=Msa1331830
chr8_4 mRNA 51500110 51503025 51500110 ID=Msa1331830-mRNA-1;Parent=Msa1331830;Name=Msa1331830-mRNA-1;_AED=0.01;_eAED=0.01;_QI=187|1|1|1|1|1|2|324|398
chr8_4 exon 51500110 51500795 51500110 ID=Msa1331830-mRNA-1:exon:15241;Parent=Msa1331830-mRNA-1
chr8_4 exon 51502004 51503025 51502004 ID=Msa1331830-mRNA-1:exon:15242;Parent=Msa1331830-mRNA-1
chr8_4 five_prime_UTR 51500110 51500296 51500110 ID=Msa1331830-mRNA-1:five_prime_utr;Parent=Msa1331830-mRNA-1
chr8_4 CDS 51500297 51500795 51500297 ID=Msa1331830-mRNA-1:cds;Parent=Msa1331830-mRNA-1
chr8_4 CDS 51502004 51502701 51502004 ID=Msa1331830-mRNA-1:cds;Parent=Msa1331830-mRNA-1
chr8_4 three_prime_UTR 51502702 51503025 51502702 ID=Msa1331830-mRNA-1:three_prime_utr;Parent=Msa1331830-mRNA-1
Gene Sequence

>Msa1331830

ATGGAAGCCGTTAAGCTAGCATCAAGTGATCCGAGCGAAACAAGAGACGCTTACAAAGTTGCTTACATAATCCATTTCTTGCTTGGAGCAGGAAACTTGCTACCTTGGAATGCATTTATCACGGCAGTTGATTACTTCGCGTACTTATATCCAACTAACCACATTGAGAAGGTTTTCGCTGTGGCTTATATGGTTTCATCTGTGCTTGTACTTCTTGCGATGATGAGTTGGGGAGGTTGGAGTAAAACAACACTGAGGTTGCGAATGAACTTAGGATTTTCAATGTTTGTTATGTCTCTCATGGTAGCTCCTGTCATTGATTGGGCCTCGAGTAGGGACGAGATGAAAGAGAGGCCAAGTGGTGCTTATGGCATGACAGTTGCAGCTGTTGTAATATGTGGTTTAGCAGATGGCTTGGTTGGTGGAAGTTTGATAGGATCAGCAGGAAGGCTTCCAAAACAGTATATGCAAGCTGTTTTTGCTGGAACTGCTTCCTCAGGTATTATAATTTCAATCTTGAGGATAATAACCAAAGCATCACTACCTCAAACCCCAAAGGGTCTCAAAATAAGTGCTCACTTGTACTTCATGGTGGCCATAGTTTTCCTCCTATGTTGCATTGTCTTTAGCAACTTGCAGCACAAGTTACCTATCATGCAGCAGTATCATCAGAGTGTACTTCAGGAGAGTCCCTTATGTTCAGGGACAAAGTTTTGTGCAGTGGCAGGGAAAATTAAGGGACCAGCTTTTGGAATATTCATAATCTATATAGTAACTCTATCTATCTTCCCAGGATTTATTGCTGAAGATCTTGAATCCAAGGCTCTTAAGGATTGGTATCCTATTATATTGATTACAGTTTATAATTTAGCTGATCTTATGGGGAAATCTTTAACTGCATTCTATGTTCCACAATGTATCAAAAGGGCCATAGGTGCTGCCACAGCAAGACTATTATTCTATCCACTTTTTATAGTTTGTCTTCATGGACCAAATTGGTTGAAAACAGAAGTGCCAATGATTGTTCTTACATTTCTGCTTGGATTTACCAATGGATATCTTACTAGTGTTCTTATGATTCTAACTCCTAAGTCAGTGCATTTCTCAGAATCAGAGTTATCTGCTATTGTGATGACCGCGTTCCTCGGATTCGGCTTGGTTGGTGGTTCTGTCCTAGGTTGGTTCTGGATACTGTGA
Protein sequence

>Msa1331830

MEAVKLASSDPSETRDAYKVAYIIHFLLGAGNLLPWNAFITAVDYFAYLYPTNHIEKVFAVAYMVSSVLVLLAMMSWGGWSKTTLRLRMNLGFSMFVMSLMVAPVIDWASSRDEMKERPSGAYGMTVAAVVICGLADGLVGGSLIGSAGRLPKQYMQAVFAGTASSGIIISILRIITKASLPQTPKGLKISAHLYFMVAIVFLLCCIVFSNLQHKLPIMQQYHQSVLQESPLCSGTKFCAVAGKIKGPAFGIFIIYIVTLSIFPGFIAEDLESKALKDWYPIILITVYNLADLMGKSLTAFYVPQCIKRAIGAATARLLFYPLFIVCLHGPNWLKTEVPMIVLTFLLGFTNGYLTSVLMILTPKSVHFSESELSAIVMTAFLGFGLVGGSVLGWFWIL*