AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0903310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0903310 MtrunA17_Chr2g0325301 93.919 148 9 0 1 148 1 148 3.11e-101 286
Msa0903310 MtrunA17_Chr6g0451421 56.552 145 61 1 1 145 1 143 8.30e-54 166
Msa0903310 MtrunA17_Chr6g0451411 58.824 136 56 0 10 145 176 311 7.09e-53 169
Msa0903310 MtrunA17_Chr2g0329701 50.714 140 69 0 9 148 233 372 2.51e-46 154
Msa0903310 MtrunA17_Chr7g0264421 51.095 137 67 0 9 145 203 339 7.40e-46 152
Msa0903310 MtrunA17_Chr6g0451441 45.033 151 75 1 3 145 313 463 1.13e-37 133
Msa0903310 MtrunA17_Chr6g0451451 53.659 82 38 0 64 145 1 82 1.25e-26 95.9
Msa0903310 MtrunA17_Chr1g0158081 37.815 119 72 1 15 133 1211 1327 4.68e-17 77.0
Msa0903310 MtrunA17_Chr3g0110591 36.975 119 73 1 15 133 27 143 5.24e-16 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0903310 AT5G51080.3 50.704 142 70 0 4 145 116 257 2.17e-45 149
Msa0903310 AT3G01410.2 48.529 136 70 0 10 145 154 289 4.14e-45 149
Msa0903310 AT3G01410.1 48.529 136 70 0 10 145 154 289 4.14e-45 149
Msa0903310 AT5G51080.1 50.704 142 70 0 4 145 179 320 1.09e-44 149
Msa0903310 AT5G51080.2 50.704 142 70 0 4 145 179 320 1.09e-44 149
Msa0903310 AT1G24090.1 51.449 138 67 0 8 145 214 351 1.05e-41 142

Find 113 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCATAGGTTCCTTAAATT+TGG 0.076392 6_2:-421478 None:intergenic
ATTTGATGCAAATTACTTTA+TGG 0.123177 6_2:-422622 None:intergenic
AGCCTACTAGTTTAGCATTT+AGG 0.177082 6_2:+422245 Msa0903310:three_prime_UTR
CGTTGTATAATTAATGTATT+TGG 0.178319 6_2:+422760 Msa0903310:three_prime_UTR
TTAGAAGCAATTAATTAAAT+TGG 0.201484 6_2:-423333 None:intergenic
GCCTACTAGTTTAGCATTTA+GGG 0.220101 6_2:+422246 Msa0903310:three_prime_UTR
TGAGTATAATGGTCTTCTTT+TGG 0.243282 6_2:+421095 Msa0903310:CDS
CTATGCACTTACACCTTGTT+TGG 0.255931 6_2:+423306 Msa0903310:three_prime_UTR
ACTCTAATGTCTTACTATTA+AGG 0.269489 6_2:+423160 Msa0903310:three_prime_UTR
CAAAAGTTTAACAAATTTAT+CGG 0.281331 6_2:-422566 None:intergenic
AGTGATGAAATGCATATTTA+CGG 0.281985 6_2:+423052 Msa0903310:three_prime_UTR
TCTTGTATTGTTGAGTTTGA+TGG 0.294112 6_2:+420870 Msa0903310:CDS
TTTGATGCAAATTACTTTAT+GGG 0.295133 6_2:-422621 None:intergenic
CTCTCAGCTTGTTTGCAATC+AGG 0.296249 6_2:+421170 Msa0903310:CDS
ATTGCTTAAGAGGCTTAAAA+TGG 0.304687 6_2:+422358 Msa0903310:three_prime_UTR
CTATGTTACTTGTCTTGTTT+AGG 0.312433 6_2:+422219 Msa0903310:three_prime_UTR
TGCAACAGTTTGAGGGTTCC+TGG 0.316314 6_2:+421443 Msa0903310:intron
GAGTCAACAACCCAAATTTA+AGG 0.325164 6_2:+421467 Msa0903310:CDS
TGCATAGGTTCCTTAAATTT+GGG 0.326607 6_2:-421477 None:intergenic
TTCGATTCCTGACTCATGTT+TGG 0.338288 6_2:-423413 None:intergenic
TTCTCATTGTGATACTCATT+TGG 0.345635 6_2:-422936 None:intergenic
GTCTTACTATTAAGGTCCTA+TGG 0.348026 6_2:+423168 Msa0903310:three_prime_UTR
TGTATGACCATGGTAGGTTT+TGG 0.348487 6_2:+422519 Msa0903310:three_prime_UTR
GCATAGGGTGGAACACGTTT+AGG 0.353946 6_2:-423289 None:intergenic
ATATTCCACCATTGCTTAAG+AGG 0.372656 6_2:+422348 Msa0903310:three_prime_UTR
CCCTTCAGTATGCATTTCAT+TGG 0.377694 6_2:+422989 Msa0903310:three_prime_UTR
AATAATTCTGCTGAGTATAA+TGG 0.382560 6_2:+421084 Msa0903310:CDS
GGAGTATCTCTCAATGGTTT+AGG 0.382630 6_2:+422859 Msa0903310:three_prime_UTR
GCTTCTAACATATGCATTTG+TGG 0.387373 6_2:+423348 Msa0903310:three_prime_UTR
GTGAATTATATCATTTCAAC+CGG 0.389133 6_2:+423381 Msa0903310:three_prime_UTR
GCTCCTGCACCAGACCTTCC+AGG 0.402463 6_2:-420909 None:intergenic
CGAATTAGTGAGAATAACTA+TGG 0.411791 6_2:-423201 None:intergenic
GAGCAAACTGAGAGAACCTT+AGG 0.422024 6_2:+422588 Msa0903310:three_prime_UTR
TCTCTCAATGGTTTAGGTTG+TGG 0.424967 6_2:+422865 Msa0903310:three_prime_UTR
ATTTGGGTTGTTGACTCTCC+AGG 0.442621 6_2:-421461 None:intergenic
TATGTCTCTTTATAGGTCCA+TGG 0.450698 6_2:+421033 Msa0903310:intron
AGAGTAACTTCAAGTCAGTC+AGG 0.457011 6_2:+421518 Msa0903310:CDS
GGAGCATCCAGTGGAAATCC+TGG 0.459158 6_2:+420891 Msa0903310:CDS
TCCGTTCTGCAGATGGGAGT+CGG 0.461814 6_2:+420937 Msa0903310:CDS
ATTTAAACCCCTTTGATATG+TGG 0.462207 6_2:-423443 None:intergenic
ACCTCTGACCACATATCAAA+GGG 0.463459 6_2:+423435 Msa0903310:three_prime_UTR
CATGAATATGCTCATATCCT+TGG 0.464886 6_2:-421137 None:intergenic
GTTTAGGTTGTGGAGTAGCT+AGG 0.468401 6_2:+422875 Msa0903310:three_prime_UTR
CCCCTAAATGCTAAACTAGT+AGG 0.469952 6_2:-422247 None:intergenic
ATAGTGGACTTAAATAAGTT+AGG 0.472307 6_2:+422907 Msa0903310:three_prime_UTR
AGTGGAAATCCTGGAAGGTC+TGG 0.475520 6_2:+420900 Msa0903310:CDS
CATGGTGTCAGCCAAGGACT+TGG 0.478840 6_2:+421051 Msa0903310:CDS
TCAGGGTCGAACATGTTCCT+AGG 0.482331 6_2:+421536 Msa0903310:CDS
CGTTTAGGAAGAATACTAGT+TGG 0.484030 6_2:-423274 None:intergenic
GCATACTGAAGGGACTTCTC+TGG 0.489897 6_2:-422979 None:intergenic
TAATGTTTGAATTTCATGCT+TGG 0.494753 6_2:+422389 Msa0903310:three_prime_UTR
CCGTTCTGCAGATGGGAGTC+GGG 0.496764 6_2:+420938 Msa0903310:CDS
TTGTTTGAGTGCCAAGTCCT+TGG 0.498512 6_2:-421062 None:intergenic
TATGCCATGGCGAACATGAC+TGG 0.508165 6_2:+422485 Msa0903310:three_prime_UTR
TATGGGTGGTGATTGACCTA+AGG 0.517361 6_2:-422604 None:intergenic
TGCTTATAAATGTGACTGGA+GGG 0.522885 6_2:+422838 Msa0903310:three_prime_UTR
AAGATGAGAATCACACATAC+AGG 0.525806 6_2:-423076 None:intergenic
GAACCTATGCAATGAGGCTG+TGG 0.532829 6_2:+421489 Msa0903310:CDS
GCTCCACAGCCTCATTGCAT+AGG 0.541509 6_2:-421492 None:intergenic
AAACAAGGTGTAAGTGCATA+GGG 0.541750 6_2:-423304 None:intergenic
ATGTGACACTGCATGTCAAA+GGG 0.541910 6_2:+422279 Msa0903310:three_prime_UTR
TATGTAAACGCGAAGATGAA+AGG 0.543732 6_2:+422707 Msa0903310:three_prime_UTR
CTGTTATCCGTTCTGCAGAT+GGG 0.545170 6_2:+420931 Msa0903310:CDS
CAAACAAGGTGTAAGTGCAT+AGG 0.547897 6_2:-423305 None:intergenic
GCTGTTATCCGTTCTGCAGA+TGG 0.548893 6_2:+420930 Msa0903310:CDS
TTAAGCCTCTTAAGCAATGG+TGG 0.548954 6_2:-422353 None:intergenic
CATGTGACACTGCATGTCAA+AGG 0.549075 6_2:+422278 Msa0903310:three_prime_UTR
TCTTACTATTAAGGTCCTAT+GGG 0.550777 6_2:+423169 Msa0903310:three_prime_UTR
CTGGAGGGAGTATCTCTCAA+TGG 0.552317 6_2:+422853 Msa0903310:three_prime_UTR
AGACCTTCCAGGATTTCCAC+TGG 0.556723 6_2:-420898 None:intergenic
ACCAATGAAATGCATACTGA+AGG 0.561481 6_2:-422990 None:intergenic
AATCCTGGAAGGTCTGGTGC+AGG 0.565510 6_2:+420906 Msa0903310:CDS
TTAAATTGGTTGTCCAAACA+AGG 0.566502 6_2:-423319 None:intergenic
CCCCTTTGATATGTGGTCAG+AGG 0.566595 6_2:-423436 None:intergenic
TAATATCTTGTGATATGCCA+TGG 0.567729 6_2:+422472 Msa0903310:three_prime_UTR
TATTAAGGTCCTATGGGATG+AGG 0.571787 6_2:+423175 Msa0903310:three_prime_UTR
ATGGTGGAATATACACATCA+TGG 0.573485 6_2:-422337 None:intergenic
AACCTCTGACCACATATCAA+AGG 0.573941 6_2:+423434 Msa0903310:three_prime_UTR
GTAGTAATATTCAACTTCGC+CGG 0.574495 6_2:-422156 None:intergenic
CCAATGAAATGCATACTGAA+GGG 0.576051 6_2:-422989 None:intergenic
ACTAAGTTACTCATTTACAC+TGG 0.580060 6_2:-423243 None:intergenic
CCTACTAGTTTAGCATTTAG+GGG 0.580750 6_2:+422247 Msa0903310:three_prime_UTR
CTACTAGTTTAGCATTTAGG+GGG 0.582134 6_2:+422248 Msa0903310:three_prime_UTR
CATAAAACCAAAACCTACCA+TGG 0.584026 6_2:-422526 None:intergenic
CTGAAAGAAGCTAGCAACCA+AGG 0.584237 6_2:+421120 Msa0903310:CDS
TAGGTCCATGGTGTCAGCCA+AGG 0.586553 6_2:+421045 Msa0903310:intron
CAGGGTCGAACATGTTCCTA+GGG 0.587867 6_2:+421537 Msa0903310:CDS
AGCTAGGTTTCTGAACATAG+TGG 0.590371 6_2:+422891 Msa0903310:three_prime_UTR
CTATGGATGCCTCATCCCAT+AGG 0.590801 6_2:-423184 None:intergenic
TTGCTTATAAATGTGACTGG+AGG 0.593049 6_2:+422837 Msa0903310:three_prime_UTR
GTTTAATGTATGACCATGGT+AGG 0.593786 6_2:+422513 Msa0903310:three_prime_UTR
TTCTTAGAGTGAATAATGTG+AGG 0.606656 6_2:+423020 Msa0903310:three_prime_UTR
CAAGTCCTTGGCTGACACCA+TGG 0.609890 6_2:-421050 None:intergenic
AGCATGCATTACTCAAGACA+TGG 0.615653 6_2:-422673 None:intergenic
CCATCCAGTCATGTTCGCCA+TGG 0.629927 6_2:-422489 None:intergenic
CATTACTCAAGACATGGGAC+AGG 0.632885 6_2:-422667 None:intergenic
CCCGACTCCCATCTGCAGAA+CGG 0.635424 6_2:-420938 None:intergenic
TTTAAGGAACCTATGCAATG+AGG 0.642695 6_2:+421483 Msa0903310:CDS
GATGCAAATTACTTTATGGG+TGG 0.644660 6_2:-422618 None:intergenic
GAGTTTGATGGAGCATCCAG+TGG 0.644852 6_2:+420882 Msa0903310:CDS
GCTGATGCTCAAGCAAATCG+CGG 0.646058 6_2:+421917 Msa0903310:CDS
GAGTAACTTCAAGTCAGTCA+GGG 0.650851 6_2:+421519 Msa0903310:CDS
CATCCAGTGGAAATCCTGGA+AGG 0.657104 6_2:+420895 Msa0903310:CDS
ATGCATTTGTGGCACACACA+AGG 0.670324 6_2:+423359 Msa0903310:three_prime_UTR
TATGAGCATATTCATGTCCG+AGG 0.670788 6_2:+421144 Msa0903310:CDS
CCATGGCGAACATGACTGGA+TGG 0.671111 6_2:+422489 Msa0903310:three_prime_UTR
GAAATTCATAAACATTCACA+TGG 0.685025 6_2:-422415 None:intergenic
GCATGCATTACTCAAGACAT+GGG 0.690542 6_2:-422672 None:intergenic
CAAGGTGTAAGTGCATAGGG+TGG 0.699219 6_2:-423301 None:intergenic
AAACAAGCTGAGAGTCACCT+CGG 0.700596 6_2:-421161 None:intergenic
CCTCTGACCACATATCAAAG+GGG 0.741805 6_2:+423436 Msa0903310:three_prime_UTR
TGGTGTTTAATGTATGACCA+TGG 0.742844 6_2:+422509 Msa0903310:three_prime_UTR
GAGTCGTGGAAACAACAGAG+AGG 0.767113 6_2:+419007 Msa0903310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTCAATTAAAATTACA+AGG - chr6_2:419613-419632 None:intergenic 10.0%
!!! GACTTTAAATAATTTTTTAA+CGG - chr6_2:419934-419953 None:intergenic 10.0%
!!! TTGAATTATTTAAATTTTTC+GGG + chr6_2:419973-419992 Msa0903310:intron 10.0%
!! TATAAAAAAATGATATAAGC+CGG - chr6_2:419549-419568 None:intergenic 15.0%
!!! AGTTCTTATTTTATTTTTCT+TGG + chr6_2:419822-419841 Msa0903310:intron 15.0%
!!! TTTTTAACGGTTAATTTTAA+TGG - chr6_2:419921-419940 None:intergenic 15.0%
!!! AATTATTTAAATTTTTCGGT+TGG + chr6_2:419976-419995 Msa0903310:intron 15.0%
!!! ATTATTTAAATTTTTCGGTT+GGG + chr6_2:419977-419996 Msa0903310:intron 15.0%
!!! TTTTATTCAATTTTAAGTCT+TGG - chr6_2:420035-420054 None:intergenic 15.0%
!!! GATTTTATATAATTTTGTGT+CGG - chr6_2:420141-420160 None:intergenic 15.0%
!!! GACATAATTGTTTTTTTTTT+TGG - chr6_2:420261-420280 None:intergenic 15.0%
!!! TGTAATATTTTGTAAACAAT+TGG - chr6_2:421281-421300 None:intergenic 15.0%
!! ATTGTTTACAAAATATTACA+TGG + chr6_2:421281-421300 Msa0903310:intron 15.0%
!!! ATTTACCATCATATTTTTAT+TGG + chr6_2:421417-421436 Msa0903310:intron 15.0%
!!! TTTATTTTCATGTTTGTATT+TGG + chr6_2:422086-422105 Msa0903310:intron 15.0%
!! CAAAAGTTTAACAAATTTAT+CGG - chr6_2:422569-422588 None:intergenic 15.0%
!! TTAGAAGCAATTAATTAAAT+TGG - chr6_2:423336-423355 None:intergenic 15.0%
!!! CAAATCATTTTTAAATCGAA+CGG - chr6_2:419289-419308 None:intergenic 20.0%
!! TAAAACATAATCCAAATTTG+CGG - chr6_2:419460-419479 None:intergenic 20.0%
!! AGCTTATAGTTTATATTTAC+CGG + chr6_2:419527-419546 Msa0903310:intron 20.0%
!! AAAATGATAAGTTATAAGCT+AGG - chr6_2:419573-419592 None:intergenic 20.0%
!!! TAATGTTTTATACTATAAGC+TGG + chr6_2:419657-419676 Msa0903310:intron 20.0%
!! TAATAAGAGATAAACATTAC+CGG - chr6_2:420220-420239 None:intergenic 20.0%
!!! TGTTTTTTTTTTTGGTGAAA+GGG - chr6_2:420253-420272 None:intergenic 20.0%
!!! TTGTTTTTTTTTTTGGTGAA+AGG - chr6_2:420254-420273 None:intergenic 20.0%
!!! ATGAAAATTTTGTCATGTTT+AGG + chr6_2:420356-420375 Msa0903310:intron 20.0%
!!! TTCAAAGTTATGTAAGTTTT+TGG + chr6_2:420412-420431 Msa0903310:intron 20.0%
!!! AATTTGAAGAAATGCTATTT+TGG + chr6_2:420555-420574 Msa0903310:intron 20.0%
!! TGCAATTCTAAGTAATTATA+TGG + chr6_2:421309-421328 Msa0903310:intron 20.0%
!! CAAAACCAATAAAAATATGA+TGG - chr6_2:421425-421444 None:intergenic 20.0%
!!! TTATTGCTATTTCATTTACT+TGG - chr6_2:422010-422029 None:intergenic 20.0%
!!! TTTTTTTTAAAAAGCAGCTT+TGG - chr6_2:422040-422059 None:intergenic 20.0%
!! GCTGCAAAAAAAAAAAATAT+AGG - chr6_2:422137-422156 None:intergenic 20.0%
!!! ATATTTTTTTTTTTTGCAGC+CGG + chr6_2:422137-422156 Msa0903310:intron 20.0%
!!! TTCTATTTTTTGTCTTCATT+TGG + chr6_2:422435-422454 Msa0903310:three_prime_UTR 20.0%
!! TTTGATGCAAATTACTTTAT+GGG - chr6_2:422624-422643 None:intergenic 20.0%
!! ATTTGATGCAAATTACTTTA+TGG - chr6_2:422625-422644 None:intergenic 20.0%
!! CGTTGTATAATTAATGTATT+TGG + chr6_2:422760-422779 Msa0903310:three_prime_UTR 20.0%
!! TTCTACTCTTTTTCTGTTTA+TGG - chr6_2:418980-418999 None:intergenic 25.0%
! CGTTCGATTTAAAAATGATT+TGG + chr6_2:419287-419306 Msa0903310:intron 25.0%
! ATCAAAATTTAGAGATTGGA+CGG - chr6_2:419330-419349 None:intergenic 25.0%
! TTCATCAGTTATCAATGATT+AGG + chr6_2:419420-419439 Msa0903310:intron 25.0%
! TAAATAACAAGCACTTATAG+TGG - chr6_2:419744-419763 None:intergenic 25.0%
! AAAGAAGTTTATGCTTATTG+TGG + chr6_2:419848-419867 Msa0903310:intron 25.0%
!!! TATAATTTTGTGTCGGTTTT+AGG - chr6_2:420134-420153 None:intergenic 25.0%
!!! AAAGTTATGTAAGTTTTTGG+TGG + chr6_2:420415-420434 Msa0903310:intron 25.0%
! ACAATACACATTATAGTTGT+AGG + chr6_2:420632-420651 Msa0903310:intron 25.0%
!! CATTTGAAGTGTAAATTACA+TGG - chr6_2:420710-420729 None:intergenic 25.0%
! TTTATAGCCATACATACATT+AGG - chr6_2:420737-420756 None:intergenic 25.0%
!! CATGATAAGTATTTTATCAG+AGG + chr6_2:420822-420841 Msa0903310:intron 25.0%
! AACACTTTATGTCTCTTTAT+AGG + chr6_2:421026-421045 Msa0903310:intron 25.0%
! AATAATTCTGCTGAGTATAA+TGG + chr6_2:421084-421103 Msa0903310:CDS 25.0%
! ATTTAAGTGGTACTTTGTAA+GGG - chr6_2:421202-421221 None:intergenic 25.0%
! AATTTAAGTGGTACTTTGTA+AGG - chr6_2:421203-421222 None:intergenic 25.0%
!!! TTGTTCTGGAAAACTAAAAA+AGG + chr6_2:421647-421666 Msa0903310:intron 25.0%
!!! ACTCGTTTTTTTATTGTTGA+AGG + chr6_2:421880-421899 Msa0903310:intron 25.0%
!!! CTCGTTTTTTTATTGTTGAA+GGG + chr6_2:421881-421900 Msa0903310:intron 25.0%
!! TTTTCATGTTTGTATTTGGA+AGG + chr6_2:422090-422109 Msa0903310:intron 25.0%
! TTTCATGTTTGTATTTGGAA+GGG + chr6_2:422091-422110 Msa0903310:intron 25.0%
! TAATGTTTGAATTTCATGCT+TGG + chr6_2:422389-422408 Msa0903310:three_prime_UTR 25.0%
! GAAATTCATAAACATTCACA+TGG - chr6_2:422418-422437 None:intergenic 25.0%
!! ATTTTGCTTATAAATGTGAC+TGG + chr6_2:422834-422853 Msa0903310:three_prime_UTR 25.0%
!!! ATAGTGGACTTAAATAAGTT+AGG + chr6_2:422907-422926 Msa0903310:three_prime_UTR 25.0%
! AGTGATGAAATGCATATTTA+CGG + chr6_2:423052-423071 Msa0903310:three_prime_UTR 25.0%
! ACTCTAATGTCTTACTATTA+AGG + chr6_2:423160-423179 Msa0903310:three_prime_UTR 25.0%
! GTGAATTATATCATTTCAAC+CGG + chr6_2:423381-423400 Msa0903310:three_prime_UTR 25.0%
! TCATGTTTGGAAAAAAAATC+CGG - chr6_2:423403-423422 None:intergenic 25.0%
TGACTAAATGCAAACGAAAA+TGG - chr6_2:419109-419128 None:intergenic 30.0%
CGTTTGTTTAGAGAATTGTT+AGG + chr6_2:419133-419152 Msa0903310:intron 30.0%
CTCGATCAAAATTTAGAGAT+TGG - chr6_2:419334-419353 None:intergenic 30.0%
ATCAATGATTAGGATCTGTT+TGG + chr6_2:419430-419449 Msa0903310:intron 30.0%
AGTTTATGCTTATTGTGGTT+AGG + chr6_2:419853-419872 Msa0903310:intron 30.0%
GAGCCAGAAAAAAAAAGTTT+GGG + chr6_2:419891-419910 Msa0903310:intron 30.0%
TATACCACTTTGTTGGAATT+TGG + chr6_2:420097-420116 Msa0903310:intron 30.0%
!!! TTAAGCCTAGTTTTAGTGTA+TGG + chr6_2:420499-420518 Msa0903310:intron 30.0%
!!! TAAGCCTAGTTTTAGTGTAT+GGG + chr6_2:420500-420519 Msa0903310:intron 30.0%
ATAACCCATACACTAAAACT+AGG - chr6_2:420507-420526 None:intergenic 30.0%
ATGTCATCCTAATGTATGTA+TGG + chr6_2:420727-420746 Msa0903310:intron 30.0%
! CGAAATTGATTTCCTCTATA+AGG + chr6_2:420764-420783 Msa0903310:intron 30.0%
TCTTGTATTGTTGAGTTTGA+TGG + chr6_2:420870-420889 Msa0903310:CDS 30.0%
! TGAGTATAATGGTCTTCTTT+TGG + chr6_2:421095-421114 Msa0903310:CDS 30.0%
! GAGTATAATGGTCTTCTTTT+GGG + chr6_2:421096-421115 Msa0903310:CDS 30.0%
! AGTATAATGGTCTTCTTTTG+GGG + chr6_2:421097-421116 Msa0903310:CDS 30.0%
TCATTCAGATGCAATTTAAG+TGG - chr6_2:421215-421234 None:intergenic 30.0%
ATTCATATTCTGCCTTTGTT+AGG + chr6_2:421341-421360 Msa0903310:intron 30.0%
TGCATAGGTTCCTTAAATTT+GGG - chr6_2:421480-421499 None:intergenic 30.0%
TTGCATAGGTTCCTTAAATT+TGG - chr6_2:421481-421500 None:intergenic 30.0%
ATCACATGACTAATTTAGAG+AGG - chr6_2:421759-421778 None:intergenic 30.0%
!!! TGTGATCCACATGTTTTAAT+AGG + chr6_2:421773-421792 Msa0903310:intron 30.0%
! GTTTTGCCTATTAAAACATG+TGG - chr6_2:421782-421801 None:intergenic 30.0%
! AGACTTTTCTGAGTTCTTAT+AGG + chr6_2:421967-421986 Msa0903310:intron 30.0%
TTCATGTTTGTATTTGGAAG+GGG + chr6_2:422092-422111 Msa0903310:intron 30.0%
!! CTATGTTACTTGTCTTGTTT+AGG + chr6_2:422219-422238 Msa0903310:three_prime_UTR 30.0%
! ATTTTAAGCCTCTTAAGCAA+TGG - chr6_2:422359-422378 None:intergenic 30.0%
ATTGCTTAAGAGGCTTAAAA+TGG + chr6_2:422358-422377 Msa0903310:three_prime_UTR 30.0%
TAATATCTTGTGATATGCCA+TGG + chr6_2:422472-422491 Msa0903310:three_prime_UTR 30.0%
!!! AGGTTTTGGTTTTATGCAAT+TGG + chr6_2:422533-422552 Msa0903310:three_prime_UTR 30.0%
TTCTCATTGTGATACTCATT+TGG - chr6_2:422939-422958 None:intergenic 30.0%
TTCTTAGAGTGAATAATGTG+AGG + chr6_2:423020-423039 Msa0903310:three_prime_UTR 30.0%
TCTTACTATTAAGGTCCTAT+GGG + chr6_2:423169-423188 Msa0903310:three_prime_UTR 30.0%
CGAATTAGTGAGAATAACTA+TGG - chr6_2:423204-423223 None:intergenic 30.0%
ACTAAGTTACTCATTTACAC+TGG - chr6_2:423246-423265 None:intergenic 30.0%
TTAAATTGGTTGTCCAAACA+AGG - chr6_2:423322-423341 None:intergenic 30.0%
!!! TTTTTTTCCAAACATGAGTC+AGG + chr6_2:423406-423425 Msa0903310:three_prime_UTR 30.0%
AGTAGAAAAATCATGAGTCG+TGG + chr6_2:418993-419012 Msa0903310:exon 35.0%
ATGACACATGCATACAGTTA+TGG - chr6_2:419204-419223 None:intergenic 35.0%
! AACTGTATGCATGTGTCATT+TGG + chr6_2:419205-419224 Msa0903310:intron 35.0%
!! CATGTGTCATTTGGAAGTAA+CGG + chr6_2:419214-419233 Msa0903310:intron 35.0%
GGAGCCAGAAAAAAAAAGTT+TGG + chr6_2:419890-419909 Msa0903310:intron 35.0%
!!! CCACCCAAACTTTTTTTTTC+TGG - chr6_2:419897-419916 None:intergenic 35.0%
CCAGAAAAAAAAAGTTTGGG+TGG + chr6_2:419894-419913 Msa0903310:intron 35.0%
TACACCACTTTGCTAGAATT+TGG + chr6_2:420004-420023 Msa0903310:intron 35.0%
TGATCCAAATTCTAGCAAAG+TGG - chr6_2:420011-420030 None:intergenic 35.0%
ATTCCAACAAAGTGGTATAG+TGG - chr6_2:420096-420115 None:intergenic 35.0%
TGATCCAAATTCCAACAAAG+TGG - chr6_2:420104-420123 None:intergenic 35.0%
!!! TTTGTGTCGGTTTTAGGTTT+CGG - chr6_2:420128-420147 None:intergenic 35.0%
TAAGAGATAAACATTACCGG+TGG - chr6_2:420217-420236 None:intergenic 35.0%
GCATAAAATCTACGCTAAAG+TGG + chr6_2:420314-420333 Msa0903310:intron 35.0%
!!! AAGCTATTTTTCCACATGAG+TGG + chr6_2:420592-420611 Msa0903310:intron 35.0%
CCTTACGAGTTAAATATGAC+TGG - chr6_2:420795-420814 None:intergenic 35.0%
CCAGTCATATTTAACTCGTA+AGG + chr6_2:420792-420811 Msa0903310:intron 35.0%
TATGTCTCTTTATAGGTCCA+TGG + chr6_2:421033-421052 Msa0903310:intron 35.0%
CATGAATATGCTCATATCCT+TGG - chr6_2:421140-421159 None:intergenic 35.0%
ATCTGAACACTACCTAACAA+AGG - chr6_2:421356-421375 None:intergenic 35.0%
!!! ATTGGTTTTGCAACAGTTTG+AGG + chr6_2:421435-421454 Msa0903310:intron 35.0%
!!! TTGGTTTTGCAACAGTTTGA+GGG + chr6_2:421436-421455 Msa0903310:intron 35.0%
GAGTCAACAACCCAAATTTA+AGG + chr6_2:421467-421486 Msa0903310:CDS 35.0%
TTTAAGGAACCTATGCAATG+AGG + chr6_2:421483-421502 Msa0903310:CDS 35.0%
GGTATACACTTTGTCTCATT+TGG + chr6_2:421558-421577 Msa0903310:intron 35.0%
GAACAAAACACAATCAAGAG+AGG - chr6_2:421633-421652 None:intergenic 35.0%
!! CTCTTGATTGTGTTTTGTTC+TGG + chr6_2:421633-421652 Msa0903310:intron 35.0%
TGGAAAACTAAAAAAGGTCG+AGG + chr6_2:421653-421672 Msa0903310:intron 35.0%
GTAGTAATATTCAACTTCGC+CGG - chr6_2:422159-422178 None:intergenic 35.0%
ACTCCACTGTCAAAAACTAT+TGG + chr6_2:422193-422212 Msa0903310:three_prime_UTR 35.0%
! TTGCCAATAGTTTTTGACAG+TGG - chr6_2:422199-422218 None:intergenic 35.0%
! AGCCTACTAGTTTAGCATTT+AGG + chr6_2:422245-422264 Msa0903310:three_prime_UTR 35.0%
! GCCTACTAGTTTAGCATTTA+GGG + chr6_2:422246-422265 Msa0903310:three_prime_UTR 35.0%
! CCTACTAGTTTAGCATTTAG+GGG + chr6_2:422247-422266 Msa0903310:three_prime_UTR 35.0%
! CTACTAGTTTAGCATTTAGG+GGG + chr6_2:422248-422267 Msa0903310:three_prime_UTR 35.0%
! ATGGTGGAATATACACATCA+TGG - chr6_2:422340-422359 None:intergenic 35.0%
ATATTCCACCATTGCTTAAG+AGG + chr6_2:422348-422367 Msa0903310:three_prime_UTR 35.0%
TGGTGTTTAATGTATGACCA+TGG + chr6_2:422509-422528 Msa0903310:three_prime_UTR 35.0%
GTTTAATGTATGACCATGGT+AGG + chr6_2:422513-422532 Msa0903310:three_prime_UTR 35.0%
CATAAAACCAAAACCTACCA+TGG - chr6_2:422529-422548 None:intergenic 35.0%
GATGCAAATTACTTTATGGG+TGG - chr6_2:422621-422640 None:intergenic 35.0%
TATGTAAACGCGAAGATGAA+AGG + chr6_2:422707-422726 Msa0903310:three_prime_UTR 35.0%
TTGCTTATAAATGTGACTGG+AGG + chr6_2:422837-422856 Msa0903310:three_prime_UTR 35.0%
TGCTTATAAATGTGACTGGA+GGG + chr6_2:422838-422857 Msa0903310:three_prime_UTR 35.0%
CCAATGAAATGCATACTGAA+GGG - chr6_2:422992-423011 None:intergenic 35.0%
ACCAATGAAATGCATACTGA+AGG - chr6_2:422993-423012 None:intergenic 35.0%
AAGATGAGAATCACACATAC+AGG - chr6_2:423079-423098 None:intergenic 35.0%
GTCTTACTATTAAGGTCCTA+TGG + chr6_2:423168-423187 Msa0903310:three_prime_UTR 35.0%
CGTTTAGGAAGAATACTAGT+TGG - chr6_2:423277-423296 None:intergenic 35.0%
AAACAAGGTGTAAGTGCATA+GGG - chr6_2:423307-423326 None:intergenic 35.0%
GCTTCTAACATATGCATTTG+TGG + chr6_2:423348-423367 Msa0903310:three_prime_UTR 35.0%
ACACATGCATACAGTTATGG+CGG - chr6_2:419201-419220 None:intergenic 40.0%
TGTTTGGCAAACCGCAAATT+TGG + chr6_2:419446-419465 Msa0903310:intron 40.0%
GGTTAGGTAATGTTCACTGA+CGG + chr6_2:419869-419888 Msa0903310:intron 40.0%
ATTCTAGCAAAGTGGTGTAG+TGG - chr6_2:420003-420022 None:intergenic 40.0%
GAACCACTATACCACTTTGT+TGG + chr6_2:420090-420109 Msa0903310:intron 40.0%
TATGAGCATATTCATGTCCG+AGG + chr6_2:421144-421163 Msa0903310:CDS 40.0%
AGAGTAACTTCAAGTCAGTC+AGG + chr6_2:421518-421537 Msa0903310:CDS 40.0%
GAGTAACTTCAAGTCAGTCA+GGG + chr6_2:421519-421538 Msa0903310:CDS 40.0%
TGAGACAAAGTGTATACCCT+AGG - chr6_2:421556-421575 None:intergenic 40.0%
CCCCTAAATGCTAAACTAGT+AGG - chr6_2:422250-422269 None:intergenic 40.0%
ATGTGACACTGCATGTCAAA+GGG + chr6_2:422279-422298 Msa0903310:three_prime_UTR 40.0%
TTAAGCCTCTTAAGCAATGG+TGG - chr6_2:422356-422375 None:intergenic 40.0%
! TGTATGACCATGGTAGGTTT+TGG + chr6_2:422519-422538 Msa0903310:three_prime_UTR 40.0%
GCATGCATTACTCAAGACAT+GGG - chr6_2:422675-422694 None:intergenic 40.0%
AGCATGCATTACTCAAGACA+TGG - chr6_2:422676-422695 None:intergenic 40.0%
! GGAGTATCTCTCAATGGTTT+AGG + chr6_2:422859-422878 Msa0903310:three_prime_UTR 40.0%
!! TCTCTCAATGGTTTAGGTTG+TGG + chr6_2:422865-422884 Msa0903310:three_prime_UTR 40.0%
AGCTAGGTTTCTGAACATAG+TGG + chr6_2:422891-422910 Msa0903310:three_prime_UTR 40.0%
CCCTTCAGTATGCATTTCAT+TGG + chr6_2:422989-423008 Msa0903310:three_prime_UTR 40.0%
TATTAAGGTCCTATGGGATG+AGG + chr6_2:423175-423194 Msa0903310:three_prime_UTR 40.0%
CAAACAAGGTGTAAGTGCAT+AGG - chr6_2:423308-423327 None:intergenic 40.0%
CTATGCACTTACACCTTGTT+TGG + chr6_2:423306-423325 Msa0903310:three_prime_UTR 40.0%
TTCGATTCCTGACTCATGTT+TGG - chr6_2:423416-423435 None:intergenic 40.0%
AACCTCTGACCACATATCAA+AGG + chr6_2:423434-423453 Msa0903310:three_prime_UTR 40.0%
ACCTCTGACCACATATCAAA+GGG + chr6_2:423435-423454 Msa0903310:three_prime_UTR 40.0%
GAGATAAACATTACCGGTGG+CGG - chr6_2:420214-420233 None:intergenic 45.0%
CTACGCTAAAGTGGTGTGTA+AGG + chr6_2:420323-420342 Msa0903310:intron 45.0%
CTGTTATCCGTTCTGCAGAT+GGG + chr6_2:420931-420950 Msa0903310:CDS 45.0%
TTGTTTGAGTGCCAAGTCCT+TGG - chr6_2:421065-421084 None:intergenic 45.0%
CTGAAAGAAGCTAGCAACCA+AGG + chr6_2:421120-421139 Msa0903310:CDS 45.0%
AAACAAGCTGAGAGTCACCT+CGG - chr6_2:421164-421183 None:intergenic 45.0%
! CTCTCAGCTTGTTTGCAATC+AGG + chr6_2:421170-421189 Msa0903310:CDS 45.0%
!! ATTTGGGTTGTTGACTCTCC+AGG - chr6_2:421464-421483 None:intergenic 45.0%
GCAAATCGCGGCAAAAATCT+TGG + chr6_2:421929-421948 Msa0903310:CDS 45.0%
AATCGCGGCAAAAATCTTGG+AGG + chr6_2:421932-421951 Msa0903310:CDS 45.0%
CATGTGACACTGCATGTCAA+AGG + chr6_2:422278-422297 Msa0903310:three_prime_UTR 45.0%
GAGCAAACTGAGAGAACCTT+AGG + chr6_2:422588-422607 Msa0903310:three_prime_UTR 45.0%
TATGGGTGGTGATTGACCTA+AGG - chr6_2:422607-422626 None:intergenic 45.0%
CATTACTCAAGACATGGGAC+AGG - chr6_2:422670-422689 None:intergenic 45.0%
!! GTTTAGGTTGTGGAGTAGCT+AGG + chr6_2:422875-422894 Msa0903310:three_prime_UTR 45.0%
ATGCATTTGTGGCACACACA+AGG + chr6_2:423359-423378 Msa0903310:three_prime_UTR 45.0%
!! AATTTAAATAATTCAATTAT+AGG - chr6_2:419969-419988 None:intergenic 5.0%
!!! ATTGAATTATTTAAATTTTT+CGG + chr6_2:419972-419991 Msa0903310:intron 5.0%
!! AATTTAAATAATTCAATTAT+AGG - chr6_2:419969-419988 None:intergenic 5.0%
!!! ATTGAATTATTTAAATTTTT+CGG + chr6_2:419972-419991 Msa0903310:intron 5.0%
GAGTCGTGGAAACAACAGAG+AGG + chr6_2:419007-419026 Msa0903310:CDS 50.0%
GGGGTGGGATCAGCTATTAT+AGG - chr6_2:420176-420195 None:intergenic 50.0%
!! AAGAGCGTTGTCCACTCATG+TGG - chr6_2:420606-420625 None:intergenic 50.0%
TGACTGGCAACGCCTTATAG+AGG - chr6_2:420779-420798 None:intergenic 50.0%
! GAGTTTGATGGAGCATCCAG+TGG + chr6_2:420882-420901 Msa0903310:CDS 50.0%
CATCCAGTGGAAATCCTGGA+AGG + chr6_2:420895-420914 Msa0903310:CDS 50.0%
AGACCTTCCAGGATTTCCAC+TGG - chr6_2:420901-420920 None:intergenic 50.0%
AGTGGAAATCCTGGAAGGTC+TGG + chr6_2:420900-420919 Msa0903310:CDS 50.0%
GCTGTTATCCGTTCTGCAGA+TGG + chr6_2:420930-420949 Msa0903310:CDS 50.0%
TGCAACAGTTTGAGGGTTCC+TGG + chr6_2:421443-421462 Msa0903310:intron 50.0%
GAACCTATGCAATGAGGCTG+TGG + chr6_2:421489-421508 Msa0903310:CDS 50.0%
TCAGGGTCGAACATGTTCCT+AGG + chr6_2:421536-421555 Msa0903310:CDS 50.0%
CAGGGTCGAACATGTTCCTA+GGG + chr6_2:421537-421556 Msa0903310:CDS 50.0%
GCTGATGCTCAAGCAAATCG+CGG + chr6_2:421917-421936 Msa0903310:CDS 50.0%
TATGCCATGGCGAACATGAC+TGG + chr6_2:422485-422504 Msa0903310:three_prime_UTR 50.0%
!! CTGGAGGGAGTATCTCTCAA+TGG + chr6_2:422853-422872 Msa0903310:three_prime_UTR 50.0%
GCATACTGAAGGGACTTCTC+TGG - chr6_2:422982-423001 None:intergenic 50.0%
CTATGGATGCCTCATCCCAT+AGG - chr6_2:423187-423206 None:intergenic 50.0%
GCATAGGGTGGAACACGTTT+AGG - chr6_2:423292-423311 None:intergenic 50.0%
CAAGGTGTAAGTGCATAGGG+TGG - chr6_2:423304-423323 None:intergenic 50.0%
CCCCTTTGATATGTGGTCAG+AGG - chr6_2:423439-423458 None:intergenic 50.0%
! GGAGCATCCAGTGGAAATCC+TGG + chr6_2:420891-420910 Msa0903310:CDS 55.0%
AATCCTGGAAGGTCTGGTGC+AGG + chr6_2:420906-420925 Msa0903310:CDS 55.0%
TCCGTTCTGCAGATGGGAGT+CGG + chr6_2:420937-420956 Msa0903310:CDS 55.0%
! TAGGTCCATGGTGTCAGCCA+AGG + chr6_2:421045-421064 Msa0903310:intron 55.0%
CAAGTCCTTGGCTGACACCA+TGG - chr6_2:421053-421072 None:intergenic 55.0%
! CATGGTGTCAGCCAAGGACT+TGG + chr6_2:421051-421070 Msa0903310:CDS 55.0%
GCTCCACAGCCTCATTGCAT+AGG - chr6_2:421495-421514 None:intergenic 55.0%
CCATCCAGTCATGTTCGCCA+TGG - chr6_2:422492-422511 None:intergenic 55.0%
CCATGGCGAACATGACTGGA+TGG + chr6_2:422489-422508 Msa0903310:three_prime_UTR 55.0%
ATCCCACCCCAGCCCATGAA+GGG + chr6_2:420186-420205 Msa0903310:intron 60.0%
CCCGACTCCCATCTGCAGAA+CGG - chr6_2:420941-420960 None:intergenic 60.0%
CCGTTCTGCAGATGGGAGTC+GGG + chr6_2:420938-420957 Msa0903310:CDS 60.0%
GATCCCACCCCAGCCCATGA+AGG + chr6_2:420185-420204 Msa0903310:intron 65.0%
!! AGCCCTTCATGGGCTGGGGT+GGG - chr6_2:420191-420210 None:intergenic 65.0%
GCTCCTGCACCAGACCTTCC+AGG - chr6_2:420912-420931 None:intergenic 65.0%
!! GAGCCCTTCATGGGCTGGGG+TGG - chr6_2:420192-420211 None:intergenic 70.0%
! GCGGAGCCCTTCATGGGCTG+GGG - chr6_2:420195-420214 None:intergenic 70.0%
GGCGGAGCCCTTCATGGGCT+GGG - chr6_2:420196-420215 None:intergenic 70.0%
TGGCGGAGCCCTTCATGGGC+TGG - chr6_2:420197-420216 None:intergenic 70.0%
!! CCGGTGGCGGAGCCCTTCAT+GGG - chr6_2:420201-420220 None:intergenic 70.0%
CCCATGAAGGGCTCCGCCAC+CGG + chr6_2:420198-420217 Msa0903310:intron 70.0%
!! ACCGGTGGCGGAGCCCTTCA+TGG - chr6_2:420202-420221 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 418956 423458 418956 ID=Msa0903310;Name=Msa0903310
chr6_2 mRNA 418956 423458 418956 ID=Msa0903310-mRNA-1;Parent=Msa0903310;Name=Msa0903310-mRNA-1;_AED=0.04;_eAED=0.04;_QI=49|1|1|1|1|1|6|1277|148
chr6_2 exon 418956 419028 418956 ID=Msa0903310-mRNA-1:exon:203;Parent=Msa0903310-mRNA-1
chr6_2 exon 420867 420959 420867 ID=Msa0903310-mRNA-1:exon:204;Parent=Msa0903310-mRNA-1
chr6_2 exon 421048 421191 421048 ID=Msa0903310-mRNA-1:exon:205;Parent=Msa0903310-mRNA-1
chr6_2 exon 421451 421558 421451 ID=Msa0903310-mRNA-1:exon:206;Parent=Msa0903310-mRNA-1
chr6_2 exon 421902 421953 421902 ID=Msa0903310-mRNA-1:exon:207;Parent=Msa0903310-mRNA-1
chr6_2 exon 422156 423458 422156 ID=Msa0903310-mRNA-1:exon:208;Parent=Msa0903310-mRNA-1
chr6_2 five_prime_UTR 418956 419004 418956 ID=Msa0903310-mRNA-1:five_prime_utr;Parent=Msa0903310-mRNA-1
chr6_2 CDS 419005 419028 419005 ID=Msa0903310-mRNA-1:cds;Parent=Msa0903310-mRNA-1
chr6_2 CDS 420867 420959 420867 ID=Msa0903310-mRNA-1:cds;Parent=Msa0903310-mRNA-1
chr6_2 CDS 421048 421191 421048 ID=Msa0903310-mRNA-1:cds;Parent=Msa0903310-mRNA-1
chr6_2 CDS 421451 421558 421451 ID=Msa0903310-mRNA-1:cds;Parent=Msa0903310-mRNA-1
chr6_2 CDS 421902 421953 421902 ID=Msa0903310-mRNA-1:cds;Parent=Msa0903310-mRNA-1
chr6_2 CDS 422156 422181 422156 ID=Msa0903310-mRNA-1:cds;Parent=Msa0903310-mRNA-1
chr6_2 three_prime_UTR 422182 423458 422182 ID=Msa0903310-mRNA-1:three_prime_utr;Parent=Msa0903310-mRNA-1
Gene Sequence

>Msa0903310

ATGAGTCGTGGAAACAACAGAGAGCGTTCTTGTATTGTTGAGTTTGATGGAGCATCCAGTGGAAATCCTGGAAGGTCTGGTGCAGGAGCTGTTATCCGTTCTGCAGATGGGAGTCGGGTCCATGGTGTCAGCCAAGGACTTGGCACTCAAACAAATAATTCTGCTGAGTATAATGGTCTTCTTTTGGGGCTGAAAGAAGCTAGCAACCAAGGATATGAGCATATTCATGTCCGAGGTGACTCTCAGCTTGTTTGCAATCAGTTTGAGGGTTCCTGGAGAGTCAACAACCCAAATTTAAGGAACCTATGCAATGAGGCTGTGGAGCTGAAGAGTAACTTCAAGTCAGTCAGGGTCGAACATGTTCCTAGGGGTTCTAACTCTGCAGCTGATGCTCAAGCAAATCGCGGCAAAAATCTTGGAGCCGGCGAAGTTGAATATTACTACTGA
Protein sequence

>Msa0903310

MSRGNNRERSCIVEFDGASSGNPGRSGAGAVIRSADGSRVHGVSQGLGTQTNNSAEYNGLLLGLKEASNQGYEHIHVRGDSQLVCNQFEGSWRVNNPNLRNLCNEAVELKSNFKSVRVEHVPRGSNSAADAQANRGKNLGAGEVEYYY*