AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1300830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1300830 MtrunA17_Chr4g0063911 98.995 398 4 0 1 398 1 398 0.0 803
Msa1300830 MtrunA17_Chr4g0020331 49.239 394 186 4 15 398 26 415 2.37e-127 373
Msa1300830 MtrunA17_Chr3g0103741 45.700 407 202 7 7 398 21 423 5.21e-114 339
Msa1300830 MtrunA17_Chr4g0022871 27.660 423 263 7 6 398 1 410 6.19e-49 171
Msa1300830 MtrunA17_Chr4g0022861 28.814 413 237 10 23 398 2 394 1.49e-46 164
Msa1300830 MtrunA17_Chr2g0323381 29.882 425 251 12 6 398 1 410 3.59e-46 163
Msa1300830 MtrunA17_Chr2g0280971 28.431 408 250 9 21 398 44 439 3.77e-46 164
Msa1300830 MtrunA17_Chr2g0280991 28.671 429 250 12 7 398 10 419 1.23e-45 162
Msa1300830 MtrunA17_Chr4g0022801 32.014 278 142 8 156 398 136 401 1.38e-26 110
Msa1300830 MtrunA17_Chr1g0184961 28.378 222 136 3 81 279 131 352 6.19e-23 99.0
Msa1300830 MtrunA17_Chr8g0335821 26.075 372 230 10 57 396 2 360 2.34e-22 97.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1300830 AT1G02630.1 63.520 392 129 5 10 398 9 389 1.58e-178 502
Msa1300830 AT1G02630.2 55.867 392 115 6 10 398 9 345 9.80e-146 417
Msa1300830 AT1G70330.1 49.114 395 186 4 16 398 59 450 4.08e-123 364
Msa1300830 AT3G09990.1 28.193 415 242 12 21 398 18 413 8.08e-44 157
Msa1300830 AT1G61630.1 27.378 431 249 9 9 398 5 412 5.95e-43 155
Msa1300830 AT4G05120.1 28.271 428 258 11 3 398 2 412 1.87e-42 154
Msa1300830 AT4G05120.2 28.271 428 258 11 3 398 2 412 1.87e-42 154
Msa1300830 AT4G05110.3 28.162 419 254 11 11 398 10 412 1.36e-41 152
Msa1300830 AT4G05110.1 28.162 419 254 11 11 398 10 412 1.36e-41 152
Msa1300830 AT4G05110.2 28.162 419 254 11 11 398 10 412 2.78e-41 151
Msa1300830 AT4G05130.1 27.791 421 253 12 11 398 10 412 1.76e-40 149
Msa1300830 AT4G05140.1 26.744 430 259 12 5 398 4 413 5.96e-38 142

Find 105 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTAAGGGACCAGCTTT+TGG 0.257914 8_3:-62509281 Msa1300830:CDS
TTATAATTTAGCTGATCTTA+TGG 0.261483 8_3:-62509150 Msa1300830:CDS
TGCAGTGGCAGGGAAAATTA+AGG 0.277433 8_3:-62509294 Msa1300830:CDS
GTTCTGTCCTAGGTTGGTTC+TGG 0.300139 8_3:-62508845 Msa1300830:CDS
TCTTGAATCCAAGGCTCTTA+AGG 0.316097 8_3:-62509201 Msa1300830:CDS
TAAACTGTAATCAATATAAT+AGG 0.319839 8_3:+62509170 None:intergenic
TGTCCACACTTCTCATGATT+TGG 0.326312 8_3:+62508703 None:intergenic
AATGTAATGAATCTATAAAC+TGG 0.326934 8_3:-62508645 Msa1300830:three_prime_UTR
ACAGCTTGCATATACTGTTT+TGG 0.330078 8_3:+62511454 None:intergenic
TATAATTTAGCTGATCTTAT+GGG 0.341304 8_3:-62509149 Msa1300830:CDS
TGTGAAGGCTTGTTTAGTTC+AGG 0.343097 8_3:-62508818 Msa1300830:exon
TACATAATCCATTTCTTGCT+TGG 0.351729 8_3:-62511844 Msa1300830:CDS
CAGGAGAGTCCCTTATGTTC+AGG 0.355351 8_3:-62509329 Msa1300830:CDS
GTATCATCAGAGTGTACTTC+AGG 0.361010 8_3:-62509348 Msa1300830:CDS
TGAGAAGTGTGGACATTGTT+AGG 0.373983 8_3:-62508695 Msa1300830:three_prime_UTR
TGGTAGCTCCTGTCATTGAT+TGG 0.376095 8_3:-62511606 Msa1300830:CDS
GAGAGGCCAAGTGGTGCTTA+TGG 0.382783 8_3:-62511559 Msa1300830:CDS
TTGGTTGGTGGTTCTGTCCT+AGG 0.383076 8_3:-62508855 Msa1300830:CDS
AAACTTTGTCCCTGAACATA+AGG 0.390189 8_3:+62509319 None:intergenic
TGATGATACTGCTGCATGAT+AGG 0.394711 8_3:+62509362 None:intergenic
TACAAGTAGAATTCTACTTA+TGG 0.398475 8_3:+62512081 None:intergenic
TTTGTCTTCATGGACCAAAT+TGG 0.398789 8_3:-62509034 Msa1300830:CDS
ACCTTCTCAATGTGGTTAGT+TGG 0.405196 8_3:+62511757 None:intergenic
TTCCTGCTCCAAGCAAGAAA+TGG 0.405518 8_3:+62511836 None:intergenic
CTCGGATTCGGCTTGGTTGG+TGG 0.408001 8_3:-62508867 Msa1300830:CDS
TTGAGACCCTTTGGGGTTTG+AGG 0.410939 8_3:+62509467 None:intergenic
TCTTGCTGTGGCAGCACCTA+TGG 0.413596 8_3:+62509082 None:intergenic
AAGTGCTCACTTGTACTTCA+TGG 0.413740 8_3:-62509441 Msa1300830:CDS
ATCCATTTCTTGCTTGGAGC+AGG 0.419667 8_3:-62511838 Msa1300830:CDS
TATCGCAAAAGGCAATCTCT+TGG 0.420101 8_3:-62508556 Msa1300830:three_prime_UTR
ATGACCGCGTTCCTCGGATT+CGG 0.421562 8_3:-62508879 Msa1300830:CDS
ATGTTCCACAATGTATCAAA+AGG 0.427242 8_3:-62509106 Msa1300830:CDS
TTGGTTGGTGGAAGTTTGAT+AGG 0.433123 8_3:-62511493 Msa1300830:CDS
ACTTCTTGCGATGATGAGTT+GGG 0.436575 8_3:-62511698 Msa1300830:CDS
TTTGCTGGAACTGCTTCCTC+AGG 0.440158 8_3:-62511430 Msa1300830:intron
ATACCAATCCTTAAGAGCCT+TGG 0.444568 8_3:+62509193 None:intergenic
GAGCAGGAAACTTGCTACCT+TGG 0.445287 8_3:-62511822 Msa1300830:CDS
TACTTCTTGCGATGATGAGT+TGG 0.445586 8_3:-62511699 Msa1300830:CDS
AGATCTTCAGCAATAAATCC+TGG 0.450755 8_3:+62509221 None:intergenic
GGTAGCTCCTGTCATTGATT+GGG 0.452656 8_3:-62511605 Msa1300830:CDS
GCAACATAGGAGGAAAACTA+TGG 0.455079 8_3:+62509415 None:intergenic
ATTGTTCTTACATTTCTGCT+TGG 0.455315 8_3:-62508990 Msa1300830:CDS
ATTGTGATGACCGCGTTCCT+CGG 0.461564 8_3:-62508885 Msa1300830:CDS
AATGTTTGTTATGTCTCTCA+TGG 0.465994 8_3:-62511626 Msa1300830:CDS
AATAGAAATAATATACTGTT+GGG 0.467486 8_3:-62508599 Msa1300830:three_prime_UTR
CTGAGGTTGCGAATGAACTT+AGG 0.472940 8_3:-62511655 Msa1300830:CDS
GGTTTGAGGTAGTGATGCTT+TGG 0.473253 8_3:+62509481 None:intergenic
AGGAGAGTCCCTTATGTTCA+GGG 0.476690 8_3:-62509328 Msa1300830:CDS
CGATGATGAGTTGGGGAGGT+TGG 0.479957 8_3:-62511690 Msa1300830:CDS
CATTGATTGGGCCTCGAGTA+GGG 0.484656 8_3:-62511593 Msa1300830:CDS
TCTGAGAAATGCACTGACTT+AGG 0.485958 8_3:+62508924 None:intergenic
AGAAATGTAAGAACAATCAT+TGG 0.487588 8_3:+62508996 None:intergenic
TCATTGATTGGGCCTCGAGT+AGG 0.493347 8_3:-62511594 Msa1300830:CDS
CAATAGAAATAATATACTGT+TGG 0.499673 8_3:-62508600 Msa1300830:three_prime_UTR
TAAGCGTCTCTTGTTTCGCT+CGG 0.504224 8_3:+62511877 None:intergenic
TTCCTCGGATTCGGCTTGGT+TGG 0.504238 8_3:-62508870 Msa1300830:CDS
TGCTGAAGATCTTGAATCCA+AGG 0.504556 8_3:-62509210 Msa1300830:CDS
AATCCAAGGCTCTTAAGGAT+TGG 0.505326 8_3:-62509196 Msa1300830:CDS
ATTATGAATATTCCAAAAGC+TGG 0.509136 8_3:+62509269 None:intergenic
GTTGCAGCTGTTGTAATATG+TGG 0.509743 8_3:-62511529 Msa1300830:CDS
GGTTTAGCAGATGGCTTGGT+TGG 0.510374 8_3:-62511508 Msa1300830:CDS
GCAGTGGCAGGGAAAATTAA+GGG 0.512760 8_3:-62509293 Msa1300830:CDS
AACTTTGTCCCTGAACATAA+GGG 0.516896 8_3:+62509320 None:intergenic
GTATTATAATTTCAATCTTG+AGG 0.517953 8_3:-62509511 Msa1300830:CDS
GTTGGTTCTGGATACTGTGA+AGG 0.522252 8_3:-62508833 Msa1300830:exon
TTGGTGGTTCTGTCCTAGGT+TGG 0.528954 8_3:-62508851 Msa1300830:CDS
TTAGCAGATGGCTTGGTTGG+TGG 0.535563 8_3:-62511505 Msa1300830:CDS
TTCTACTTATGGTGATAAAG+TGG 0.535803 8_3:+62512092 None:intergenic
AACACTAGTAAGATATCCAT+TGG 0.535884 8_3:+62508962 None:intergenic
TCACTACCTCAAACCCCAAA+GGG 0.535947 8_3:-62509473 Msa1300830:CDS
TTTCTGCTTGGATTTACCAA+TGG 0.538009 8_3:-62508978 Msa1300830:CDS
GGAAGTTTGATAGGATCAGC+AGG 0.544382 8_3:-62511484 Msa1300830:CDS
CAATGTATCAAAAGGGCCAT+AGG 0.548021 8_3:-62509098 Msa1300830:CDS
CTCGAGGCCCAATCAATGAC+AGG 0.549987 8_3:+62511598 None:intergenic
ATTTACTTCATTCAGAACTT+CGG 0.551328 8_3:-62511964 Msa1300830:five_prime_UTR
ATAATTTAGCTGATCTTATG+GGG 0.556528 8_3:-62509148 Msa1300830:CDS
TGTTCCACAATGTATCAAAA+GGG 0.559037 8_3:-62509105 Msa1300830:CDS
GTAATATGTGGTTTAGCAGA+TGG 0.566070 8_3:-62511517 Msa1300830:CDS
TGCTAAAGACAATGCAACAT+AGG 0.569551 8_3:+62509402 None:intergenic
GATCTTCAGCAATAAATCCT+GGG 0.574505 8_3:+62509222 None:intergenic
TACTCATAGAAGAGACGAGA+TGG 0.574855 8_3:-62508523 Msa1300830:three_prime_UTR
ATGTGGTTTAGCAGATGGCT+TGG 0.575031 8_3:-62511512 Msa1300830:CDS
GTAACTCTATCTATCTTCCC+AGG 0.575256 8_3:-62509239 Msa1300830:CDS
ACCTTGGAATGCATTTATCA+CGG 0.589722 8_3:-62511806 Msa1300830:CDS
TAAAGACAATGCAACATAGG+AGG 0.600160 8_3:+62509405 None:intergenic
CACCAACCAAGCCGAATCCG+AGG 0.600577 8_3:+62508868 None:intergenic
ATGTAATGAATCTATAAACT+GGG 0.601993 8_3:-62508644 Msa1300830:three_prime_UTR
TCCAACTAACCACATTGAGA+AGG 0.605730 8_3:-62511758 Msa1300830:CDS
ATCACTACCTCAAACCCCAA+AGG 0.611165 8_3:-62509474 Msa1300830:CDS
GTCATGCCATAAGCACCACT+TGG 0.614144 8_3:+62511553 None:intergenic
ATATAGAAACAATCTACTTG+AGG 0.614819 8_3:+62508748 None:intergenic
ACAGTATCCAGAACCAACCT+AGG 0.614984 8_3:+62508838 None:intergenic
CTTGCGATGATGAGTTGGGG+AGG 0.619198 8_3:-62511694 Msa1300830:CDS
TTTCATCTCGTCCCTACTCG+AGG 0.623295 8_3:+62511582 None:intergenic
GTTTGATAGGATCAGCAGGA+AGG 0.624638 8_3:-62511480 Msa1300830:CDS
GCCGTGATAAATGCATTCCA+AGG 0.626474 8_3:+62511805 None:intergenic
GTAGGGACGAGATGAAAGAG+AGG 0.629913 8_3:-62511576 Msa1300830:CDS
ATAGAATAATAGTCTTGCTG+TGG 0.649512 8_3:+62509070 None:intergenic
CTTCTTGCGATGATGAGTTG+GGG 0.656503 8_3:-62511697 Msa1300830:CDS
CAGCGAAAACCTTCTCAATG+TGG 0.674870 8_3:+62511749 None:intergenic
GTGCCAAATCATGAGAAGTG+TGG 0.697483 8_3:-62508706 Msa1300830:three_prime_UTR
TGCTCACTTGTACTTCATGG+TGG 0.701698 8_3:-62509438 Msa1300830:CDS
CAAGCCGAATCCGAGGAACG+CGG 0.703497 8_3:+62508875 None:intergenic
GAGATGAAAGAGAGGCCAAG+TGG 0.710667 8_3:-62511568 Msa1300830:CDS
GTTGGAGTAAAACAACACTG+AGG 0.825176 8_3:-62511672 Msa1300830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACAACATATATATTAAAAT+TGG - chr8_3:62510132-62510151 Msa1300830:intron 10.0%
!! ACAACATATATATTAAAATT+GGG - chr8_3:62510133-62510152 Msa1300830:intron 10.0%
!!! TCAAGAATAATATTTTTTTT+GGG + chr8_3:62510334-62510353 None:intergenic 10.0%
!!! TTCAAGAATAATATTTTTTT+TGG + chr8_3:62510335-62510354 None:intergenic 10.0%
!!! ATAATTTTTATTGTTGAAAA+AGG + chr8_3:62510532-62510551 None:intergenic 10.0%
!! TTATTAATCTATTATTAAAC+AGG - chr8_3:62510631-62510650 Msa1300830:intron 10.0%
!! ACCTAAAAAATTATAATTTA+TGG - chr8_3:62510847-62510866 Msa1300830:intron 10.0%
!! CCTAAAAAATTATAATTTAT+GGG - chr8_3:62510848-62510867 Msa1300830:intron 10.0%
!!! TAAAATTATTTTGAAGATGT+TGG + chr8_3:62508575-62508594 None:intergenic 15.0%
!! TATAAGAAAGTGAAAAAATA+TGG - chr8_3:62508683-62508702 Msa1300830:three_prime_UTR 15.0%
!!! TTTTAGATAGTAATGTAAAA+AGG + chr8_3:62509261-62509280 None:intergenic 15.0%
!!! TAAATTTTTTGTTACAATGA+CGG + chr8_3:62509775-62509794 None:intergenic 15.0%
!! TAAATCTATACCATAATTTA+TGG - chr8_3:62510044-62510063 Msa1300830:intron 15.0%
!!! TTTTCCAAATTTCATTTTAA+AGG - chr8_3:62510107-62510126 Msa1300830:intron 15.0%
!! ATCAAGACAATTAAATTAAA+AGG - chr8_3:62510196-62510215 Msa1300830:intron 15.0%
!!! CAAGAATAATATTTTTTTTG+GGG + chr8_3:62510333-62510352 None:intergenic 15.0%
!! AATATTATTCTTGAATTAGT+CGG - chr8_3:62510340-62510359 Msa1300830:intron 15.0%
!! TAGCTCAATATTTATTATTT+GGG + chr8_3:62510387-62510406 None:intergenic 15.0%
!! TACAAATAAATCTATCAATT+TGG + chr8_3:62510468-62510487 None:intergenic 15.0%
!! TAATCTCTAACAAAAAATTA+AGG + chr8_3:62510750-62510769 None:intergenic 15.0%
!!! CCCATAAATTATAATTTTTT+AGG + chr8_3:62510851-62510870 None:intergenic 15.0%
!! TAAACTGTAATCAATATAAT+AGG + chr8_3:62511442-62511461 None:intergenic 15.0%
!! AATAGAAATAATATACTGTT+GGG - chr8_3:62512010-62512029 Msa1300830:five_prime_UTR 15.0%
!!! TTTTCAAAAATAGAGTGAAA+GGG + chr8_3:62508622-62508641 None:intergenic 20.0%
!! ATTTAACAACATTTCTAACT+AGG + chr8_3:62509321-62509340 None:intergenic 20.0%
!! ATTCATGTATAAATTCAAGT+GGG + chr8_3:62509723-62509742 None:intergenic 20.0%
!! TATTCATGTATAAATTCAAG+TGG + chr8_3:62509724-62509743 None:intergenic 20.0%
!! AAATTAGTTGCCATAAATTA+TGG + chr8_3:62510057-62510076 None:intergenic 20.0%
!!! TATGCCTTTAAAATGAAATT+TGG + chr8_3:62510114-62510133 None:intergenic 20.0%
!!! ATTTTTTTTTAGAGAAAAGC+AGG - chr8_3:62510270-62510289 Msa1300830:intron 20.0%
!!! TTTTTTTTTAGAGAAAAGCA+GGG - chr8_3:62510271-62510290 Msa1300830:intron 20.0%
!! AGCTCAATATTTATTATTTG+GGG + chr8_3:62510386-62510405 None:intergenic 20.0%
!! GTAGCTCAATATTTATTATT+TGG + chr8_3:62510388-62510407 None:intergenic 20.0%
!!! TTTTGCAACTAATTTTGTTT+GGG + chr8_3:62510411-62510430 None:intergenic 20.0%
!! ATAGTCATAGACAAATTAAA+CGG - chr8_3:62510560-62510579 Msa1300830:intron 20.0%
!!! AATTGCTATTTTAAAACGTT+GGG + chr8_3:62510692-62510711 None:intergenic 20.0%
!!! AAATTGCTATTTTAAAACGT+TGG + chr8_3:62510693-62510712 None:intergenic 20.0%
!! AAAAAATCCTTCTTTATAGT+TGG - chr8_3:62510915-62510934 Msa1300830:intron 20.0%
!!! TCATATTATAATACCTTTTG+TGG - chr8_3:62511068-62511087 Msa1300830:intron 20.0%
!! GTATTATAATTTCAATCTTG+AGG - chr8_3:62511098-62511117 Msa1300830:intron 20.0%
!! TTATAATTTAGCTGATCTTA+TGG - chr8_3:62511459-62511478 Msa1300830:CDS 20.0%
!! TATAATTTAGCTGATCTTAT+GGG - chr8_3:62511460-62511479 Msa1300830:CDS 20.0%
!! AATGTAATGAATCTATAAAC+TGG - chr8_3:62511964-62511983 Msa1300830:five_prime_UTR 20.0%
!! ATGTAATGAATCTATAAACT+GGG - chr8_3:62511965-62511984 Msa1300830:five_prime_UTR 20.0%
!! CAATAGAAATAATATACTGT+TGG - chr8_3:62512009-62512028 Msa1300830:five_prime_UTR 20.0%
! TACAAGTAGAATTCTACTTA+TGG + chr8_3:62508531-62508550 None:intergenic 25.0%
!! CTTTTCAAAAATAGAGTGAA+AGG + chr8_3:62508623-62508642 None:intergenic 25.0%
! ATTTACTTCATTCAGAACTT+CGG - chr8_3:62508645-62508664 Msa1300830:three_prime_UTR 25.0%
! GTAAAATTGATCAACTATGA+GGG + chr8_3:62509235-62509254 None:intergenic 25.0%
! AGTAAAATTGATCAACTATG+AGG + chr8_3:62509236-62509255 None:intergenic 25.0%
! ATAAAATACTCATTGTCGTA+TGG + chr8_3:62509523-62509542 None:intergenic 25.0%
! AAGTATATACAAGCATTTGA+AGG - chr8_3:62509549-62509568 Msa1300830:intron 25.0%
!!! GCATTTGTCTTTTTTTCTAA+AGG - chr8_3:62509635-62509654 Msa1300830:intron 25.0%
!!! TTGTCTTTTTTTCTAAAGGA+TGG - chr8_3:62509639-62509658 Msa1300830:intron 25.0%
!!! TGTCTTTTTTTCTAAAGGAT+GGG - chr8_3:62509640-62509659 Msa1300830:intron 25.0%
!!! TTTTTTTTAGAGAAAAGCAG+GGG - chr8_3:62510272-62510291 Msa1300830:intron 25.0%
!!! TTTGCAACTAATTTTGTTTG+GGG + chr8_3:62510410-62510429 None:intergenic 25.0%
!! CTTTTGCAACTAATTTTGTT+TGG + chr8_3:62510412-62510431 None:intergenic 25.0%
! TTGTATTCGTTTGATACTTT+CGG - chr8_3:62510483-62510502 Msa1300830:intron 25.0%
! AAACAGGCGTATTAAATTTA+AGG + chr8_3:62510892-62510911 None:intergenic 25.0%
!!! AGAAGGATTTTTTTGAAAAC+AGG + chr8_3:62510908-62510927 None:intergenic 25.0%
! ATTATGAATATTCCAAAAGC+TGG + chr8_3:62511343-62511362 None:intergenic 25.0%
! ATAATTTAGCTGATCTTATG+GGG - chr8_3:62511461-62511480 Msa1300830:CDS 25.0%
! TGAAGACAAACTATAAAAAG+TGG + chr8_3:62511565-62511584 None:intergenic 25.0%
!!! CTTTTTATAGTTTGTCTTCA+TGG - chr8_3:62511565-62511584 Msa1300830:CDS 25.0%
! AGAAATGTAAGAACAATCAT+TGG + chr8_3:62511616-62511635 None:intergenic 25.0%
! ATATAGAAACAATCTACTTG+AGG + chr8_3:62511864-62511883 None:intergenic 25.0%
TTCTACTTATGGTGATAAAG+TGG + chr8_3:62508520-62508539 None:intergenic 30.0%
TACATAATCCATTTCTTGCT+TGG - chr8_3:62508765-62508784 Msa1300830:three_prime_UTR 30.0%
!! AATGTTTGTTATGTCTCTCA+TGG - chr8_3:62508983-62509002 Msa1300830:CDS 30.0%
! TTGTGTTGTAATTTTACCTG+AGG + chr8_3:62509198-62509217 None:intergenic 30.0%
! CTATCTAAAACAATGTCCTT+AGG - chr8_3:62509271-62509290 Msa1300830:CDS 30.0%
! CCTTAGAATTTGTTCGAATA+AGG + chr8_3:62509446-62509465 None:intergenic 30.0%
CCTTATTCGAACAAATTCTA+AGG - chr8_3:62509443-62509462 Msa1300830:CDS 30.0%
AAAAACTTTGCCTTAGATGA+AGG - chr8_3:62509472-62509491 Msa1300830:CDS 30.0%
TTTCATGTATCCTTCATCTA+AGG + chr8_3:62509485-62509504 None:intergenic 30.0%
ATACAAGCATTTGAAGGTTA+AGG - chr8_3:62509555-62509574 Msa1300830:intron 30.0%
TACAAGCATTTGAAGGTTAA+GGG - chr8_3:62509556-62509575 Msa1300830:intron 30.0%
!!! GTCTTTTTTTCTAAAGGATG+GGG - chr8_3:62509641-62509660 Msa1300830:intron 30.0%
!!! TCTTTTTTTCTAAAGGATGG+GGG - chr8_3:62509642-62509661 Msa1300830:intron 30.0%
!! CATAGTTTTGCTAGATGTAT+AGG + chr8_3:62509857-62509876 None:intergenic 30.0%
!!! CTTAAAGAATTTTGAAGCTG+TGG - chr8_3:62509887-62509906 Msa1300830:intron 30.0%
! TTTGCTAGGTTATAGTTTGT+AGG - chr8_3:62509936-62509955 Msa1300830:intron 30.0%
AGTCGGTATGTATTTATATC+CGG - chr8_3:62510357-62510376 Msa1300830:intron 30.0%
! TATTTATTATTTGGGGTAGC+CGG + chr8_3:62510379-62510398 None:intergenic 30.0%
ATGTTCCACAATGTATCAAA+AGG - chr8_3:62511503-62511522 Msa1300830:CDS 30.0%
TGTTCCACAATGTATCAAAA+GGG - chr8_3:62511504-62511523 Msa1300830:CDS 30.0%
! ATAGAATAATAGTCTTGCTG+TGG + chr8_3:62511542-62511561 None:intergenic 30.0%
! ACTTCTGTTTTCAACCAATT+TGG + chr8_3:62511592-62511611 None:intergenic 30.0%
ATTGTTCTTACATTTCTGCT+TGG - chr8_3:62511619-62511638 Msa1300830:CDS 30.0%
AACACTAGTAAGATATCCAT+TGG + chr8_3:62511650-62511669 None:intergenic 30.0%
! GACATTGTTAGGAAACTTTT+AGG - chr8_3:62511925-62511944 Msa1300830:exon 30.0%
! TACATGTTTTCTATCGCAAA+AGG - chr8_3:62512042-62512061 Msa1300830:five_prime_UTR 30.0%
ACCTTGGAATGCATTTATCA+CGG - chr8_3:62508803-62508822 Msa1300830:three_prime_UTR 35.0%
GTAATATGTGGTTTAGCAGA+TGG - chr8_3:62509092-62509111 Msa1300830:CDS 35.0%
! ACAGCTTGCATATACTGTTT+TGG + chr8_3:62509158-62509177 None:intergenic 35.0%
!!! TATATGCAAGCTGTTTTTGC+TGG - chr8_3:62509164-62509183 Msa1300830:CDS 35.0%
CTAAAACAATGTCCTTAGGT+AGG - chr8_3:62509275-62509294 Msa1300830:CDS 35.0%
ATTGACAACACTGATACAAG+AGG - chr8_3:62509407-62509426 Msa1300830:CDS 35.0%
TTGAAGGTTAAGGGAGAAAA+AGG - chr8_3:62509565-62509584 Msa1300830:intron 35.0%
GGAAAGTAGATTCTAGATCA+TGG - chr8_3:62509586-62509605 Msa1300830:intron 35.0%
! CTAGGTTATAGTTTGTAGGA+GGG - chr8_3:62509940-62509959 Msa1300830:intron 35.0%
TAGGAGGGACTAAAATTTGA+TGG - chr8_3:62509955-62509974 Msa1300830:intron 35.0%
!! TTGATGGCCTCTACTTATTT+TGG - chr8_3:62509971-62509990 Msa1300830:intron 35.0%
GATCTGACCAAAATAAGTAG+AGG + chr8_3:62509981-62510000 None:intergenic 35.0%
!!! TATTTTTTTTGGGGTAGCCA+AGG + chr8_3:62510324-62510343 None:intergenic 35.0%
!! AAACAGGCAGTTGATGAAAT+TGG - chr8_3:62510647-62510666 Msa1300830:intron 35.0%
!! AACAGGCAGTTGATGAAATT+GGG - chr8_3:62510648-62510667 Msa1300830:intron 35.0%
CTCGCAAAAAATTTATGCAG+GGG + chr8_3:62510941-62510960 None:intergenic 35.0%
GCTCGCAAAAAATTTATGCA+GGG + chr8_3:62510942-62510961 None:intergenic 35.0%
AGCTCGCAAAAAATTTATGC+AGG + chr8_3:62510943-62510962 None:intergenic 35.0%
TATCTATCCGAACATACTAG+CGG + chr8_3:62511002-62511021 None:intergenic 35.0%
! TAATACCTTTTGTGGTTGAC+AGG - chr8_3:62511076-62511095 Msa1300830:intron 35.0%
TAATACCTGTCAACCACAAA+AGG + chr8_3:62511084-62511103 None:intergenic 35.0%
!!! ACTTATTTTGAGACCCTTTG+GGG + chr8_3:62511152-62511171 None:intergenic 35.0%
!!! CACTTATTTTGAGACCCTTT+GGG + chr8_3:62511153-62511172 None:intergenic 35.0%
TAAAGACAATGCAACATAGG+AGG + chr8_3:62511207-62511226 None:intergenic 35.0%
TGCTAAAGACAATGCAACAT+AGG + chr8_3:62511210-62511229 None:intergenic 35.0%
AACTTTGTCCCTGAACATAA+GGG + chr8_3:62511292-62511311 None:intergenic 35.0%
AAACTTTGTCCCTGAACATA+AGG + chr8_3:62511293-62511312 None:intergenic 35.0%
! AAAATTAAGGGACCAGCTTT+TGG - chr8_3:62511328-62511347 Msa1300830:intron 35.0%
GATCTTCAGCAATAAATCCT+GGG + chr8_3:62511390-62511409 None:intergenic 35.0%
AGATCTTCAGCAATAAATCC+TGG + chr8_3:62511391-62511410 None:intergenic 35.0%
TTTGTCTTCATGGACCAAAT+TGG - chr8_3:62511575-62511594 Msa1300830:CDS 35.0%
TTTCTGCTTGGATTTACCAA+TGG - chr8_3:62511631-62511650 Msa1300830:CDS 35.0%
TCCAACTAACCACATTGAGA+AGG - chr8_3:62508851-62508870 Msa1300830:CDS 40.0%
ACCTTCTCAATGTGGTTAGT+TGG + chr8_3:62508855-62508874 None:intergenic 40.0%
TACTTCTTGCGATGATGAGT+TGG - chr8_3:62508910-62508929 Msa1300830:CDS 40.0%
ACTTCTTGCGATGATGAGTT+GGG - chr8_3:62508911-62508930 Msa1300830:CDS 40.0%
GTTGGAGTAAAACAACACTG+AGG - chr8_3:62508937-62508956 Msa1300830:CDS 40.0%
GTTGCAGCTGTTGTAATATG+TGG - chr8_3:62509080-62509099 Msa1300830:CDS 40.0%
!! TTGGTTGGTGGAAGTTTGAT+AGG - chr8_3:62509116-62509135 Msa1300830:CDS 40.0%
AGATCCACAGCTACCATTAT+GGG - chr8_3:62509611-62509630 Msa1300830:intron 40.0%
AAAGACAAATGCCCCCATAA+TGG + chr8_3:62509627-62509646 None:intergenic 40.0%
!! ACTGCTTTTCTTGCTTTGCT+AGG - chr8_3:62509922-62509941 Msa1300830:intron 40.0%
! GCTAGGTTATAGTTTGTAGG+AGG - chr8_3:62509939-62509958 Msa1300830:intron 40.0%
! ACTAACATGCTTTTCTCCAG+CGG + chr8_3:62510969-62510988 None:intergenic 40.0%
CATCTTTCCGCTAGTATGTT+CGG - chr8_3:62510992-62511011 Msa1300830:intron 40.0%
!!! GCACTTATTTTGAGACCCTT+TGG + chr8_3:62511154-62511173 None:intergenic 40.0%
AAGTGCTCACTTGTACTTCA+TGG - chr8_3:62511168-62511187 Msa1300830:intron 40.0%
GCAACATAGGAGGAAAACTA+TGG + chr8_3:62511197-62511216 None:intergenic 40.0%
TGATGATACTGCTGCATGAT+AGG + chr8_3:62511250-62511269 None:intergenic 40.0%
GTATCATCAGAGTGTACTTC+AGG - chr8_3:62511261-62511280 Msa1300830:intron 40.0%
GTAACTCTATCTATCTTCCC+AGG - chr8_3:62511370-62511389 Msa1300830:intron 40.0%
TGCTGAAGATCTTGAATCCA+AGG - chr8_3:62511399-62511418 Msa1300830:intron 40.0%
TCTTGAATCCAAGGCTCTTA+AGG - chr8_3:62511408-62511427 Msa1300830:intron 40.0%
AATCCAAGGCTCTTAAGGAT+TGG - chr8_3:62511413-62511432 Msa1300830:intron 40.0%
ATACCAATCCTTAAGAGCCT+TGG + chr8_3:62511419-62511438 None:intergenic 40.0%
! ATGGCCCTTTTGATACATTG+TGG + chr8_3:62511511-62511530 None:intergenic 40.0%
CAATGTATCAAAAGGGCCAT+AGG - chr8_3:62511511-62511530 Msa1300830:CDS 40.0%
!! TCTGAGAAATGCACTGACTT+AGG + chr8_3:62511688-62511707 None:intergenic 40.0%
! TGTGAAGGCTTGTTTAGTTC+AGG - chr8_3:62511791-62511810 Msa1300830:CDS 40.0%
TGTCCACACTTCTCATGATT+TGG + chr8_3:62511909-62511928 None:intergenic 40.0%
! TGAGAAGTGTGGACATTGTT+AGG - chr8_3:62511914-62511933 Msa1300830:exon 40.0%
TATCGCAAAAGGCAATCTCT+TGG - chr8_3:62512053-62512072 Msa1300830:five_prime_UTR 40.0%
TACTCATAGAAGAGACGAGA+TGG - chr8_3:62512086-62512105 Msa1300830:five_prime_UTR 40.0%
! TAAGCGTCTCTTGTTTCGCT+CGG + chr8_3:62508735-62508754 None:intergenic 45.0%
ATCCATTTCTTGCTTGGAGC+AGG - chr8_3:62508771-62508790 Msa1300830:three_prime_UTR 45.0%
TTCCTGCTCCAAGCAAGAAA+TGG + chr8_3:62508776-62508795 None:intergenic 45.0%
GCCGTGATAAATGCATTCCA+AGG + chr8_3:62508807-62508826 None:intergenic 45.0%
CAGCGAAAACCTTCTCAATG+TGG + chr8_3:62508863-62508882 None:intergenic 45.0%
! CATTGAGAAGGTTTTCGCTG+TGG - chr8_3:62508863-62508882 Msa1300830:CDS 45.0%
! GGTTTTCGCTGTGGCTTATA+TGG - chr8_3:62508872-62508891 Msa1300830:CDS 45.0%
CTTCTTGCGATGATGAGTTG+GGG - chr8_3:62508912-62508931 Msa1300830:CDS 45.0%
CTGAGGTTGCGAATGAACTT+AGG - chr8_3:62508954-62508973 Msa1300830:CDS 45.0%
TGGTAGCTCCTGTCATTGAT+TGG - chr8_3:62509003-62509022 Msa1300830:CDS 45.0%
! GGTAGCTCCTGTCATTGATT+GGG - chr8_3:62509004-62509023 Msa1300830:CDS 45.0%
ATGTGGTTTAGCAGATGGCT+TGG - chr8_3:62509097-62509116 Msa1300830:CDS 45.0%
GGAAGTTTGATAGGATCAGC+AGG - chr8_3:62509125-62509144 Msa1300830:CDS 45.0%
GTTTGATAGGATCAGCAGGA+AGG - chr8_3:62509129-62509148 Msa1300830:CDS 45.0%
CAGATCCACAGCTACCATTA+TGG - chr8_3:62509610-62509629 Msa1300830:intron 45.0%
GATCCACAGCTACCATTATG+GGG - chr8_3:62509612-62509631 Msa1300830:intron 45.0%
ATCCACAGCTACCATTATGG+GGG - chr8_3:62509613-62509632 Msa1300830:intron 45.0%
! GGTTTGAGGTAGTGATGCTT+TGG + chr8_3:62511131-62511150 None:intergenic 45.0%
ATCACTACCTCAAACCCCAA+AGG - chr8_3:62511135-62511154 Msa1300830:intron 45.0%
TCACTACCTCAAACCCCAAA+GGG - chr8_3:62511136-62511155 Msa1300830:intron 45.0%
TGCTCACTTGTACTTCATGG+TGG - chr8_3:62511171-62511190 Msa1300830:intron 45.0%
AGGAGAGTCCCTTATGTTCA+GGG - chr8_3:62511281-62511300 Msa1300830:intron 45.0%
!! AGGGACAAAGTTTTGTGCAG+TGG - chr8_3:62511300-62511319 Msa1300830:intron 45.0%
!! ACAAAGTTTTGTGCAGTGGC+AGG - chr8_3:62511304-62511323 Msa1300830:intron 45.0%
!! CAAAGTTTTGTGCAGTGGCA+GGG - chr8_3:62511305-62511324 Msa1300830:intron 45.0%
TGCAGTGGCAGGGAAAATTA+AGG - chr8_3:62511315-62511334 Msa1300830:intron 45.0%
GCAGTGGCAGGGAAAATTAA+GGG - chr8_3:62511316-62511335 Msa1300830:intron 45.0%
ACAGTATCCAGAACCAACCT+AGG + chr8_3:62511774-62511793 None:intergenic 45.0%
!! GTTGGTTCTGGATACTGTGA+AGG - chr8_3:62511776-62511795 Msa1300830:CDS 45.0%
GTGCCAAATCATGAGAAGTG+TGG - chr8_3:62511903-62511922 Msa1300830:CDS 45.0%
!!! AAAATTGAATAATTTTTTTA+AGG - chr8_3:62510819-62510838 Msa1300830:intron 5.0%
!! GAGCAGGAAACTTGCTACCT+TGG - chr8_3:62508787-62508806 Msa1300830:three_prime_UTR 50.0%
! TCATTGATTGGGCCTCGAGT+AGG - chr8_3:62509015-62509034 Msa1300830:CDS 50.0%
! CATTGATTGGGCCTCGAGTA+GGG - chr8_3:62509016-62509035 Msa1300830:CDS 50.0%
TTTCATCTCGTCCCTACTCG+AGG + chr8_3:62509030-62509049 None:intergenic 50.0%
GTAGGGACGAGATGAAAGAG+AGG - chr8_3:62509033-62509052 Msa1300830:CDS 50.0%
GAGATGAAAGAGAGGCCAAG+TGG - chr8_3:62509041-62509060 Msa1300830:CDS 50.0%
GTCATGCCATAAGCACCACT+TGG + chr8_3:62509059-62509078 None:intergenic 50.0%
!! GGTTTAGCAGATGGCTTGGT+TGG - chr8_3:62509101-62509120 Msa1300830:CDS 50.0%
!! TTAGCAGATGGCTTGGTTGG+TGG - chr8_3:62509104-62509123 Msa1300830:CDS 50.0%
TTTGCTGGAACTGCTTCCTC+AGG - chr8_3:62509179-62509198 Msa1300830:CDS 50.0%
CGTGCTATAGCTCCTACCTA+AGG + chr8_3:62509290-62509309 None:intergenic 50.0%
GCAGGGGCCAACTATAAAGA+AGG + chr8_3:62510925-62510944 None:intergenic 50.0%
!! TTGAGACCCTTTGGGGTTTG+AGG + chr8_3:62511145-62511164 None:intergenic 50.0%
CAGGAGAGTCCCTTATGTTC+AGG - chr8_3:62511280-62511299 Msa1300830:intron 50.0%
ATTGTGATGACCGCGTTCCT+CGG - chr8_3:62511724-62511743 Msa1300830:CDS 50.0%
!! TTGGTTGGTGGTTCTGTCCT+AGG - chr8_3:62511754-62511773 Msa1300830:CDS 50.0%
! TTGGTGGTTCTGTCCTAGGT+TGG - chr8_3:62511758-62511777 Msa1300830:CDS 50.0%
!! GTTCTGTCCTAGGTTGGTTC+TGG - chr8_3:62511764-62511783 Msa1300830:CDS 50.0%
! CTTGCGATGATGAGTTGGGG+AGG - chr8_3:62508915-62508934 Msa1300830:CDS 55.0%
! CGATGATGAGTTGGGGAGGT+TGG - chr8_3:62508919-62508938 Msa1300830:CDS 55.0%
CTCGAGGCCCAATCAATGAC+AGG + chr8_3:62509014-62509033 None:intergenic 55.0%
GAGAGGCCAAGTGGTGCTTA+TGG - chr8_3:62509050-62509069 Msa1300830:CDS 55.0%
TGCCCCCATAATGGTAGCTG+TGG + chr8_3:62509618-62509637 None:intergenic 55.0%
! AGGGGCGTAGCCCTTCATAT+GGG - chr8_3:62510290-62510309 Msa1300830:intron 55.0%
GCGTAGCCCTTCATATGGGA+GGG - chr8_3:62510294-62510313 Msa1300830:intron 55.0%
CGTAGCCCTTCATATGGGAG+GGG - chr8_3:62510295-62510314 Msa1300830:intron 55.0%
GGCTACCCCTCCCATATGAA+GGG + chr8_3:62510303-62510322 None:intergenic 55.0%
AGGCTACCCCTCCCATATGA+AGG + chr8_3:62510304-62510323 None:intergenic 55.0%
TCATATGGGAGGGGTAGCCT+TGG - chr8_3:62510304-62510323 Msa1300830:intron 55.0%
!!! TTTTTTGCGAGCTTCGCCGC+TGG - chr8_3:62510950-62510969 Msa1300830:intron 55.0%
! TCTTGCTGTGGCAGCACCTA+TGG + chr8_3:62511530-62511549 None:intergenic 55.0%
ATGACCGCGTTCCTCGGATT+CGG - chr8_3:62511730-62511749 Msa1300830:CDS 55.0%
!! TTCCTCGGATTCGGCTTGGT+TGG - chr8_3:62511739-62511758 Msa1300830:CDS 55.0%
! CAGGGGCGTAGCCCTTCATA+TGG - chr8_3:62510289-62510308 Msa1300830:intron 60.0%
GGCGTAGCCCTTCATATGGG+AGG - chr8_3:62510293-62510312 Msa1300830:intron 60.0%
CAAGCCGAATCCGAGGAACG+CGG + chr8_3:62511737-62511756 None:intergenic 60.0%
CACCAACCAAGCCGAATCCG+AGG + chr8_3:62511744-62511763 None:intergenic 60.0%
!! CTCGGATTCGGCTTGGTTGG+TGG - chr8_3:62511742-62511761 Msa1300830:CDS 60.0%
CGCGTTCCTCGGATTCGGCT+TGG - chr8_3:62511735-62511754 Msa1300830:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 62508515 62512116 62508515 ID=Msa1300830;Name=Msa1300830
chr8_3 mRNA 62508515 62512116 62508515 ID=Msa1300830-mRNA-1;Parent=Msa1300830;Name=Msa1300830-mRNA-1;_AED=0.01;_eAED=0.01;_QI=187|1|1|1|1|1|2|321|398
chr8_3 exon 62508515 62509533 62508515 ID=Msa1300830-mRNA-1:exon:18588;Parent=Msa1300830-mRNA-1
chr8_3 exon 62511431 62512116 62511431 ID=Msa1300830-mRNA-1:exon:18587;Parent=Msa1300830-mRNA-1
chr8_3 five_prime_UTR 62511930 62512116 62511930 ID=Msa1300830-mRNA-1:five_prime_utr;Parent=Msa1300830-mRNA-1
chr8_3 CDS 62511431 62511929 62511431 ID=Msa1300830-mRNA-1:cds;Parent=Msa1300830-mRNA-1
chr8_3 CDS 62508836 62509533 62508836 ID=Msa1300830-mRNA-1:cds;Parent=Msa1300830-mRNA-1
chr8_3 three_prime_UTR 62508515 62508835 62508515 ID=Msa1300830-mRNA-1:three_prime_utr;Parent=Msa1300830-mRNA-1
Gene Sequence

>Msa1300830

ATGGAAGCTGTTAAGCTAGCATCAAGTGATCCGAGCGAAACAAGAGACGCTTACAAAGTTGCTTACATAATCCATTTCTTGCTTGGAGCAGGAAACTTGCTACCTTGGAATGCATTTATCACGGCAGTTGATTACTTCGCGTACTTATATCCAACTAACCACATTGAGAAGGTTTTCGCTGTGGCTTATATGGTTTCATCTGTGCTTGTACTTCTTGCGATGATGAGTTGGGGAGGTTGGAGTAAAACAACACTGAGGTTGCGAATGAACTTAGGATTTTCAATGTTTGTTATGTCTCTCATGGTAGCTCCTGTCATTGATTGGGCCTCGAGTAGGGACGAGATGAAAGAGAGGCCAAGTGGTGCTTATGGCATGACAGTTGCAGCTGTTGTAATATGTGGTTTAGCAGATGGCTTGGTTGGTGGAAGTTTGATAGGATCAGCAGGAAGGCTTCCAAAACAGTATATGCAAGCTGTTTTTGCTGGAACTGCTTCCTCAGGTATTATAATTTCAATCTTGAGGATAATAACCAAAGCATCACTACCTCAAACCCCAAAGGGTCTCAAAATAAGTGCTCACTTGTACTTCATGGTGGCCATAGTTTTCCTCCTATGTTGCATTGTCTTTAGCAACTTGCAGCACAAGTTACCTATCATGCAGCAGTATCATCAGAGTGTACTTCAGGAGAGTCCCTTATGTTCAGGGACAAAGTTTTGTGCAGTGGCAGGGAAAATTAAGGGACCAGCTTTTGGAATATTCATAATCTATATAGTAACTCTATCTATCTTCCCAGGATTTATTGCTGAAGATCTTGAATCCAAGGCTCTTAAGGATTGGTATCCTATTATATTGATTACAGTTTATAATTTAGCTGATCTTATGGGGAAATCTTTAACTGCATTCTATGTTCCACAATGTATCAAAAGGGCCATAGGTGCTGCCACAGCAAGACTATTATTCTATCCACTTTTTATAGTTTGTCTTCATGGACCAAATTGGTTGAAAACAGAAGTGCCAATGATTGTTCTTACATTTCTGCTTGGATTTACCAATGGATATCTTACTAGTGTTCTTATGATTCTAACTCCTAAGTCAGTGCATTTCTCAGAATCAGAGTTATCTGCTATTGTGATGACCGCGTTCCTCGGATTCGGCTTGGTTGGTGGTTCTGTCCTAGGTTGGTTCTGGATACTGTGA
Protein sequence

>Msa1300830

MEAVKLASSDPSETRDAYKVAYIIHFLLGAGNLLPWNAFITAVDYFAYLYPTNHIEKVFAVAYMVSSVLVLLAMMSWGGWSKTTLRLRMNLGFSMFVMSLMVAPVIDWASSRDEMKERPSGAYGMTVAAVVICGLADGLVGGSLIGSAGRLPKQYMQAVFAGTASSGIIISILRIITKASLPQTPKGLKISAHLYFMVAIVFLLCCIVFSNLQHKLPIMQQYHQSVLQESPLCSGTKFCAVAGKIKGPAFGIFIIYIVTLSIFPGFIAEDLESKALKDWYPIILITVYNLADLMGKSLTAFYVPQCIKRAIGAATARLLFYPLFIVCLHGPNWLKTEVPMIVLTFLLGFTNGYLTSVLMILTPKSVHFSESELSAIVMTAFLGFGLVGGSVLGWFWIL*