AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1214860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1214860 MtrunA17_Chr4g0063911 98.995 398 4 0 1 398 1 398 0.0 803
Msa1214860 MtrunA17_Chr4g0020331 49.239 394 186 4 15 398 26 415 2.37e-127 373
Msa1214860 MtrunA17_Chr3g0103741 45.700 407 202 7 7 398 21 423 5.21e-114 339
Msa1214860 MtrunA17_Chr4g0022871 27.660 423 263 7 6 398 1 410 6.19e-49 171
Msa1214860 MtrunA17_Chr4g0022861 28.814 413 237 10 23 398 2 394 1.49e-46 164
Msa1214860 MtrunA17_Chr2g0323381 29.882 425 251 12 6 398 1 410 3.59e-46 163
Msa1214860 MtrunA17_Chr2g0280971 28.431 408 250 9 21 398 44 439 3.77e-46 164
Msa1214860 MtrunA17_Chr2g0280991 28.671 429 250 12 7 398 10 419 1.23e-45 162
Msa1214860 MtrunA17_Chr4g0022801 32.014 278 142 8 156 398 136 401 1.38e-26 110
Msa1214860 MtrunA17_Chr1g0184961 28.378 222 136 3 81 279 131 352 6.19e-23 99.0
Msa1214860 MtrunA17_Chr8g0335821 26.075 372 230 10 57 396 2 360 2.34e-22 97.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1214860 AT1G02630.1 63.520 392 129 5 10 398 9 389 1.58e-178 502
Msa1214860 AT1G02630.2 55.867 392 115 6 10 398 9 345 9.80e-146 417
Msa1214860 AT1G70330.1 49.114 395 186 4 16 398 59 450 4.08e-123 364
Msa1214860 AT3G09990.1 28.193 415 242 12 21 398 18 413 8.08e-44 157
Msa1214860 AT1G61630.1 27.378 431 249 9 9 398 5 412 5.95e-43 155
Msa1214860 AT4G05120.1 28.271 428 258 11 3 398 2 412 1.87e-42 154
Msa1214860 AT4G05120.2 28.271 428 258 11 3 398 2 412 1.87e-42 154
Msa1214860 AT4G05110.3 28.162 419 254 11 11 398 10 412 1.36e-41 152
Msa1214860 AT4G05110.1 28.162 419 254 11 11 398 10 412 1.36e-41 152
Msa1214860 AT4G05110.2 28.162 419 254 11 11 398 10 412 2.78e-41 151
Msa1214860 AT4G05130.1 27.791 421 253 12 11 398 10 412 1.76e-40 149
Msa1214860 AT4G05140.1 26.744 430 259 12 5 398 4 413 5.96e-38 142

Find 104 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTAAGGGACCAGCTTT+TGG 0.257914 8_1:-75694587 Msa1214860:CDS
TTATAATTTAGCTGATCTTA+TGG 0.261483 8_1:-75694456 Msa1214860:CDS
TGCAGTGGCAGGGAAAATTA+AGG 0.277433 8_1:-75694600 Msa1214860:CDS
GTTCTGTCCTAGGTTGGTTC+TGG 0.300139 8_1:-75694151 Msa1214860:CDS
GACATTGTTAGGAAACTTCT+AGG 0.308309 8_1:-75693990 Msa1214860:three_prime_UTR
TCTTGAATCCAAGGCTCTTA+AGG 0.316097 8_1:-75694507 Msa1214860:CDS
TAAACTGTAATCAATATAAT+AGG 0.319839 8_1:+75694476 None:intergenic
TGTCCACACTTCTCATGATT+TGG 0.326312 8_1:+75694009 None:intergenic
AATGTAATGAATCTATAAAC+TGG 0.326934 8_1:-75693948 Msa1214860:three_prime_UTR
ACAGCTTGCATATACTGTTT+TGG 0.330078 8_1:+75696070 None:intergenic
TATAATTTAGCTGATCTTAT+GGG 0.341304 8_1:-75694455 Msa1214860:CDS
TGTGAAGGCTTGTTTAGTTC+AGG 0.343097 8_1:-75694124 Msa1214860:exon
TACATAATCCATTTCTTGCT+TGG 0.351729 8_1:-75696460 Msa1214860:CDS
CAGGAGAGTCCCTTATGTTC+AGG 0.355351 8_1:-75694635 Msa1214860:CDS
GTATCATCAGAGTGTACTTC+AGG 0.361010 8_1:-75694654 Msa1214860:CDS
TGAGAAGTGTGGACATTGTT+AGG 0.373983 8_1:-75694001 Msa1214860:three_prime_UTR
TGGTAGCTCCTGTCATTGAT+TGG 0.376095 8_1:-75696222 Msa1214860:CDS
GAGAGGCCAAGTGGTGCTTA+TGG 0.382783 8_1:-75696175 Msa1214860:CDS
TTGGTTGGTGGTTCTGTCCT+AGG 0.383076 8_1:-75694161 Msa1214860:CDS
AAACTTTGTCCCTGAACATA+AGG 0.390189 8_1:+75694625 None:intergenic
TGATGATACTGCTGCATGAT+AGG 0.394711 8_1:+75694668 None:intergenic
TACAAGTAGAATTCTACTTA+TGG 0.398475 8_1:+75696697 None:intergenic
TTTGTCTTCATGGACCAAAT+TGG 0.398789 8_1:-75694340 Msa1214860:CDS
ACCTTCTCAATGTGGTTAGT+TGG 0.405196 8_1:+75696373 None:intergenic
TTCCTGCTCCAAGCAAGAAA+TGG 0.405518 8_1:+75696452 None:intergenic
CTCGGATTCGGCTTGGTTGG+TGG 0.408001 8_1:-75694173 Msa1214860:CDS
TTGAGACCCTTTGGGGTTTG+AGG 0.410939 8_1:+75694773 None:intergenic
TCTTGCTGTGGCAGCACCTA+TGG 0.413596 8_1:+75694388 None:intergenic
AAGTGCTCACTTGTACTTCA+TGG 0.413740 8_1:-75694747 Msa1214860:CDS
ATCCATTTCTTGCTTGGAGC+AGG 0.419667 8_1:-75696454 Msa1214860:CDS
ATGACCGCGTTCCTCGGATT+CGG 0.421562 8_1:-75694185 Msa1214860:CDS
ATGTTCCACAATGTATCAAA+AGG 0.427242 8_1:-75694412 Msa1214860:CDS
TTGGTTGGTGGAAGTTTGAT+AGG 0.433123 8_1:-75696109 Msa1214860:CDS
ACTTCTTGCGATGATGAGTT+GGG 0.436575 8_1:-75696314 Msa1214860:CDS
TTTGCTGGAACTGCTTCCTC+AGG 0.440158 8_1:-75696046 Msa1214860:intron
ATACCAATCCTTAAGAGCCT+TGG 0.444568 8_1:+75694499 None:intergenic
GAGCAGGAAACTTGCTACCT+TGG 0.445287 8_1:-75696438 Msa1214860:CDS
TACTTCTTGCGATGATGAGT+TGG 0.445586 8_1:-75696315 Msa1214860:CDS
AGATCTTCAGCAATAAATCC+TGG 0.450755 8_1:+75694527 None:intergenic
GGTAGCTCCTGTCATTGATT+GGG 0.452656 8_1:-75696221 Msa1214860:CDS
GCAACATAGGAGGAAAACTA+TGG 0.455079 8_1:+75694721 None:intergenic
ATTGTTCTTACATTTCTGCT+TGG 0.455315 8_1:-75694296 Msa1214860:CDS
ATTGTGATGACCGCGTTCCT+CGG 0.461564 8_1:-75694191 Msa1214860:CDS
AATGTTTGTTATGTCTCTCA+TGG 0.465994 8_1:-75696242 Msa1214860:CDS
CTGAGGTTGCGAATGAACTT+AGG 0.472940 8_1:-75696271 Msa1214860:CDS
GGTTTGAGGTAGTGATGCTT+TGG 0.473253 8_1:+75694787 None:intergenic
AGGAGAGTCCCTTATGTTCA+GGG 0.476690 8_1:-75694634 Msa1214860:CDS
CGATGATGAGTTGGGGAGGT+TGG 0.479957 8_1:-75696306 Msa1214860:CDS
CATTGATTGGGCCTCGAGTA+GGG 0.484656 8_1:-75696209 Msa1214860:CDS
TCTGAGAAATGCACTGACTT+AGG 0.485958 8_1:+75694230 None:intergenic
AGAAATGTAAGAACAATCAT+TGG 0.487588 8_1:+75694302 None:intergenic
CAATAGAAATAAAATGCTGT+TGG 0.489747 8_1:-75693903 Msa1214860:three_prime_UTR
AATAGAAATAAAATGCTGTT+GGG 0.489771 8_1:-75693902 Msa1214860:three_prime_UTR
TCATTGATTGGGCCTCGAGT+AGG 0.493347 8_1:-75696210 Msa1214860:CDS
TAAGCGTCTCTTGTTTCGCT+CGG 0.504224 8_1:+75696493 None:intergenic
TTCCTCGGATTCGGCTTGGT+TGG 0.504238 8_1:-75694176 Msa1214860:CDS
TGCTGAAGATCTTGAATCCA+AGG 0.504556 8_1:-75694516 Msa1214860:CDS
AATCCAAGGCTCTTAAGGAT+TGG 0.505326 8_1:-75694502 Msa1214860:CDS
ATTATGAATATTCCAAAAGC+TGG 0.509136 8_1:+75694575 None:intergenic
GTTGCAGCTGTTGTAATATG+TGG 0.509743 8_1:-75696145 Msa1214860:CDS
GGTTTAGCAGATGGCTTGGT+TGG 0.510374 8_1:-75696124 Msa1214860:CDS
GCAGTGGCAGGGAAAATTAA+GGG 0.512760 8_1:-75694599 Msa1214860:CDS
AACTTTGTCCCTGAACATAA+GGG 0.516896 8_1:+75694626 None:intergenic
GTATTATAATTTCAATCTTG+AGG 0.517953 8_1:-75694817 Msa1214860:CDS
GTTGGTTCTGGATACTGTGA+AGG 0.522252 8_1:-75694139 Msa1214860:exon
TTGGTGGTTCTGTCCTAGGT+TGG 0.528954 8_1:-75694157 Msa1214860:CDS
TTAGCAGATGGCTTGGTTGG+TGG 0.535563 8_1:-75696121 Msa1214860:CDS
TTCTACTTATGGTGATAAAG+TGG 0.535803 8_1:+75696708 None:intergenic
AACACTAGTAAGATATCCAT+TGG 0.535884 8_1:+75694268 None:intergenic
TCACTACCTCAAACCCCAAA+GGG 0.535947 8_1:-75694779 Msa1214860:CDS
TTTCTGCTTGGATTTACCAA+TGG 0.538009 8_1:-75694284 Msa1214860:CDS
GGAAGTTTGATAGGATCAGC+AGG 0.544382 8_1:-75696100 Msa1214860:CDS
CAATGTATCAAAAGGGCCAT+AGG 0.548021 8_1:-75694404 Msa1214860:CDS
CTCGAGGCCCAATCAATGAC+AGG 0.549987 8_1:+75696214 None:intergenic
ATTTACTTCATTCAGAACTT+CGG 0.551328 8_1:-75696580 Msa1214860:five_prime_UTR
ATAATTTAGCTGATCTTATG+GGG 0.556528 8_1:-75694454 Msa1214860:CDS
TGTTCCACAATGTATCAAAA+GGG 0.559037 8_1:-75694411 Msa1214860:CDS
GTAATATGTGGTTTAGCAGA+TGG 0.566070 8_1:-75696133 Msa1214860:CDS
TGCTAAAGACAATGCAACAT+AGG 0.569551 8_1:+75694708 None:intergenic
GATCTTCAGCAATAAATCCT+GGG 0.574505 8_1:+75694528 None:intergenic
ATGTGGTTTAGCAGATGGCT+TGG 0.575031 8_1:-75696128 Msa1214860:CDS
GTAACTCTATCTATCTTCCC+AGG 0.575256 8_1:-75694545 Msa1214860:CDS
ACCTTGGAATGCATTTATCA+CGG 0.589722 8_1:-75696422 Msa1214860:CDS
TAAAGACAATGCAACATAGG+AGG 0.600160 8_1:+75694711 None:intergenic
CACCAACCAAGCCGAATCCG+AGG 0.600577 8_1:+75694174 None:intergenic
ATGTAATGAATCTATAAACT+GGG 0.601993 8_1:-75693947 Msa1214860:three_prime_UTR
TCCAACTAACCACATTGAGA+AGG 0.605730 8_1:-75696374 Msa1214860:CDS
ATCACTACCTCAAACCCCAA+AGG 0.611165 8_1:-75694780 Msa1214860:CDS
GTCATGCCATAAGCACCACT+TGG 0.614144 8_1:+75696169 None:intergenic
ATATAGAAACAATCTACTTG+AGG 0.614819 8_1:+75694054 None:intergenic
ACAGTATCCAGAACCAACCT+AGG 0.614984 8_1:+75694144 None:intergenic
CTTGCGATGATGAGTTGGGG+AGG 0.619198 8_1:-75696310 Msa1214860:CDS
TTTCATCTCGTCCCTACTCG+AGG 0.623295 8_1:+75696198 None:intergenic
GTTTGATAGGATCAGCAGGA+AGG 0.624638 8_1:-75696096 Msa1214860:CDS
GCCGTGATAAATGCATTCCA+AGG 0.626474 8_1:+75696421 None:intergenic
GTAGGGACGAGATGAAAGAG+AGG 0.629913 8_1:-75696192 Msa1214860:CDS
ATAGAATAATAGTCTTGCTG+TGG 0.649512 8_1:+75694376 None:intergenic
CTTCTTGCGATGATGAGTTG+GGG 0.656503 8_1:-75696313 Msa1214860:CDS
CAGCGAAAACCTTCTCAATG+TGG 0.674870 8_1:+75696365 None:intergenic
GTGCCAAATCATGAGAAGTG+TGG 0.697483 8_1:-75694012 Msa1214860:three_prime_UTR
TGCTCACTTGTACTTCATGG+TGG 0.701698 8_1:-75694744 Msa1214860:CDS
CAAGCCGAATCCGAGGAACG+CGG 0.703497 8_1:+75694181 None:intergenic
GAGATGAAAGAGAGGCCAAG+TGG 0.710667 8_1:-75696184 Msa1214860:CDS
GTTGGAGTAAAACAACACTG+AGG 0.825176 8_1:-75696288 Msa1214860:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACAACATATATATTAAAAT+TGG - chr8_1:75695481-75695500 Msa1214860:intron 10.0%
!! ACAACATATATATTAAAATT+GGG - chr8_1:75695482-75695501 Msa1214860:intron 10.0%
!!! TAAAATTATTTTGAAGATGT+TGG + chr8_1:75693924-75693943 None:intergenic 15.0%
!! TATAAGAAAGTGAAAAAATA+TGG - chr8_1:75694032-75694051 Msa1214860:three_prime_UTR 15.0%
!!! TTTTAGATAGTAATGTAAAA+AGG + chr8_1:75694610-75694629 None:intergenic 15.0%
!!! TAAATTTTTTGTTACAATGA+CGG + chr8_1:75695124-75695143 None:intergenic 15.0%
!! TAAATCTATACCATAATTTA+TGG - chr8_1:75695393-75695412 Msa1214860:intron 15.0%
!!! TTTTCCAAATTTCATTTTAA+AGG - chr8_1:75695456-75695475 Msa1214860:intron 15.0%
!! ATCAAGACAATTAAATTAAA+AGG - chr8_1:75695545-75695564 Msa1214860:intron 15.0%
!! TAAACTGTAATCAATATAAT+AGG + chr8_1:75696101-75696120 None:intergenic 15.0%
!!! TTTTCAAAAATAGAGTGAAA+GGG + chr8_1:75693971-75693990 None:intergenic 20.0%
!! ATTTAACAACATTTCTAACT+AGG + chr8_1:75694670-75694689 None:intergenic 20.0%
!! ATTCATGTATAAATTCAAGT+GGG + chr8_1:75695072-75695091 None:intergenic 20.0%
!! TATTCATGTATAAATTCAAG+TGG + chr8_1:75695073-75695092 None:intergenic 20.0%
!! AAATTAGTTGCCATAAATTA+TGG + chr8_1:75695406-75695425 None:intergenic 20.0%
!!! TATGCCTTTAAAATGAAATT+TGG + chr8_1:75695463-75695482 None:intergenic 20.0%
!!! TCATATTATAATACCTTTTG+TGG - chr8_1:75695727-75695746 Msa1214860:intron 20.0%
!! GTATTATAATTTCAATCTTG+AGG - chr8_1:75695757-75695776 Msa1214860:intron 20.0%
!! TTATAATTTAGCTGATCTTA+TGG - chr8_1:75696118-75696137 Msa1214860:CDS 20.0%
!! TATAATTTAGCTGATCTTAT+GGG - chr8_1:75696119-75696138 Msa1214860:CDS 20.0%
!! AATGTAATGAATCTATAAAC+TGG - chr8_1:75696626-75696645 Msa1214860:five_prime_UTR 20.0%
!! ATGTAATGAATCTATAAACT+GGG - chr8_1:75696627-75696646 Msa1214860:five_prime_UTR 20.0%
!! AATAGAAATAAAATGCTGTT+GGG - chr8_1:75696672-75696691 Msa1214860:five_prime_UTR 20.0%
! TACAAGTAGAATTCTACTTA+TGG + chr8_1:75693880-75693899 None:intergenic 25.0%
!! CTTTTCAAAAATAGAGTGAA+AGG + chr8_1:75693972-75693991 None:intergenic 25.0%
! ATTTACTTCATTCAGAACTT+CGG - chr8_1:75693994-75694013 Msa1214860:three_prime_UTR 25.0%
! GTAAAATTGATCAACTATGA+GGG + chr8_1:75694584-75694603 None:intergenic 25.0%
! AGTAAAATTGATCAACTATG+AGG + chr8_1:75694585-75694604 None:intergenic 25.0%
! ATAAAATACTCATTGTCGTA+TGG + chr8_1:75694872-75694891 None:intergenic 25.0%
! AAGTATATACAAGCATTTGA+AGG - chr8_1:75694898-75694917 Msa1214860:intron 25.0%
!!! GCATTTGTCTTTTTTTCTAA+AGG - chr8_1:75694984-75695003 Msa1214860:intron 25.0%
!!! TTGTCTTTTTTTCTAAAGGA+TGG - chr8_1:75694988-75695007 Msa1214860:intron 25.0%
!!! TGTCTTTTTTTCTAAAGGAT+GGG - chr8_1:75694989-75695008 Msa1214860:intron 25.0%
! ATTATGAATATTCCAAAAGC+TGG + chr8_1:75696002-75696021 None:intergenic 25.0%
! ATAATTTAGCTGATCTTATG+GGG - chr8_1:75696120-75696139 Msa1214860:CDS 25.0%
! TGAAGACAAACTATAAAAAG+TGG + chr8_1:75696224-75696243 None:intergenic 25.0%
!!! CTTTTTATAGTTTGTCTTCA+TGG - chr8_1:75696224-75696243 Msa1214860:CDS 25.0%
! AGAAATGTAAGAACAATCAT+TGG + chr8_1:75696275-75696294 None:intergenic 25.0%
! ATATAGAAACAATCTACTTG+AGG + chr8_1:75696523-75696542 None:intergenic 25.0%
! CAATAGAAATAAAATGCTGT+TGG - chr8_1:75696671-75696690 Msa1214860:five_prime_UTR 25.0%
TTCTACTTATGGTGATAAAG+TGG + chr8_1:75693869-75693888 None:intergenic 30.0%
TACATAATCCATTTCTTGCT+TGG - chr8_1:75694114-75694133 Msa1214860:three_prime_UTR 30.0%
!! AATGTTTGTTATGTCTCTCA+TGG - chr8_1:75694332-75694351 Msa1214860:CDS 30.0%
! TTGTGTTGTAATTTTACCTG+AGG + chr8_1:75694547-75694566 None:intergenic 30.0%
! CTATCTAAAACAATGTCCTT+AGG - chr8_1:75694620-75694639 Msa1214860:CDS 30.0%
! CCTTAGAATTTGTTCGAATA+AGG + chr8_1:75694795-75694814 None:intergenic 30.0%
CCTTATTCGAACAAATTCTA+AGG - chr8_1:75694792-75694811 Msa1214860:CDS 30.0%
AAAAACTTTGCCTTAGATGA+AGG - chr8_1:75694821-75694840 Msa1214860:intron 30.0%
TTTCATGTATCCTTCATCTA+AGG + chr8_1:75694834-75694853 None:intergenic 30.0%
ATACAAGCATTTGAAGGTTA+AGG - chr8_1:75694904-75694923 Msa1214860:intron 30.0%
TACAAGCATTTGAAGGTTAA+GGG - chr8_1:75694905-75694924 Msa1214860:intron 30.0%
!!! GTCTTTTTTTCTAAAGGATG+GGG - chr8_1:75694990-75695009 Msa1214860:intron 30.0%
!!! TCTTTTTTTCTAAAGGATGG+GGG - chr8_1:75694991-75695010 Msa1214860:intron 30.0%
!! CATAGTTTTGCTAGATGTAT+AGG + chr8_1:75695206-75695225 None:intergenic 30.0%
!!! CTTAAAGAATTTTGAAGCTG+TGG - chr8_1:75695236-75695255 Msa1214860:intron 30.0%
! TTTGCTAGGTTATAGTTTGT+AGG - chr8_1:75695285-75695304 Msa1214860:intron 30.0%
ATGTTCCACAATGTATCAAA+AGG - chr8_1:75696162-75696181 Msa1214860:CDS 30.0%
TGTTCCACAATGTATCAAAA+GGG - chr8_1:75696163-75696182 Msa1214860:CDS 30.0%
! ATAGAATAATAGTCTTGCTG+TGG + chr8_1:75696201-75696220 None:intergenic 30.0%
! ACTTCTGTTTTCAACCAATT+TGG + chr8_1:75696251-75696270 None:intergenic 30.0%
ATTGTTCTTACATTTCTGCT+TGG - chr8_1:75696278-75696297 Msa1214860:CDS 30.0%
AACACTAGTAAGATATCCAT+TGG + chr8_1:75696309-75696328 None:intergenic 30.0%
ACCTTGGAATGCATTTATCA+CGG - chr8_1:75694152-75694171 Msa1214860:CDS 35.0%
GTAATATGTGGTTTAGCAGA+TGG - chr8_1:75694441-75694460 Msa1214860:CDS 35.0%
! ACAGCTTGCATATACTGTTT+TGG + chr8_1:75694507-75694526 None:intergenic 35.0%
!!! TATATGCAAGCTGTTTTTGC+TGG - chr8_1:75694513-75694532 Msa1214860:CDS 35.0%
CTAAAACAATGTCCTTAGGT+AGG - chr8_1:75694624-75694643 Msa1214860:CDS 35.0%
ATTGACAACACTGATACAAG+AGG - chr8_1:75694756-75694775 Msa1214860:CDS 35.0%
TTGAAGGTTAAGGGAGAAAA+AGG - chr8_1:75694914-75694933 Msa1214860:intron 35.0%
GGAAAGTAGATTCTAGATCA+TGG - chr8_1:75694935-75694954 Msa1214860:intron 35.0%
! CTAGGTTATAGTTTGTAGGA+GGG - chr8_1:75695289-75695308 Msa1214860:intron 35.0%
TAGGAGGGACTAAAATTTGA+TGG - chr8_1:75695304-75695323 Msa1214860:intron 35.0%
!! TTGATGGCCTCTACTTATTT+TGG - chr8_1:75695320-75695339 Msa1214860:intron 35.0%
GATCTGACCAAAATAAGTAG+AGG + chr8_1:75695330-75695349 None:intergenic 35.0%
TATCTATCCGAACATACTAG+CGG + chr8_1:75695661-75695680 None:intergenic 35.0%
! TAATACCTTTTGTGGTTGAC+AGG - chr8_1:75695735-75695754 Msa1214860:intron 35.0%
TAATACCTGTCAACCACAAA+AGG + chr8_1:75695743-75695762 None:intergenic 35.0%
!!! ACTTATTTTGAGACCCTTTG+GGG + chr8_1:75695811-75695830 None:intergenic 35.0%
!!! CACTTATTTTGAGACCCTTT+GGG + chr8_1:75695812-75695831 None:intergenic 35.0%
TAAAGACAATGCAACATAGG+AGG + chr8_1:75695866-75695885 None:intergenic 35.0%
TGCTAAAGACAATGCAACAT+AGG + chr8_1:75695869-75695888 None:intergenic 35.0%
AACTTTGTCCCTGAACATAA+GGG + chr8_1:75695951-75695970 None:intergenic 35.0%
AAACTTTGTCCCTGAACATA+AGG + chr8_1:75695952-75695971 None:intergenic 35.0%
! AAAATTAAGGGACCAGCTTT+TGG - chr8_1:75695987-75696006 Msa1214860:intron 35.0%
GATCTTCAGCAATAAATCCT+GGG + chr8_1:75696049-75696068 None:intergenic 35.0%
AGATCTTCAGCAATAAATCC+TGG + chr8_1:75696050-75696069 None:intergenic 35.0%
TTTGTCTTCATGGACCAAAT+TGG - chr8_1:75696234-75696253 Msa1214860:CDS 35.0%
TTTCTGCTTGGATTTACCAA+TGG - chr8_1:75696290-75696309 Msa1214860:CDS 35.0%
GACATTGTTAGGAAACTTCT+AGG - chr8_1:75696584-75696603 Msa1214860:five_prime_UTR 35.0%
TCCAACTAACCACATTGAGA+AGG - chr8_1:75694200-75694219 Msa1214860:CDS 40.0%
ACCTTCTCAATGTGGTTAGT+TGG + chr8_1:75694204-75694223 None:intergenic 40.0%
TACTTCTTGCGATGATGAGT+TGG - chr8_1:75694259-75694278 Msa1214860:CDS 40.0%
ACTTCTTGCGATGATGAGTT+GGG - chr8_1:75694260-75694279 Msa1214860:CDS 40.0%
GTTGGAGTAAAACAACACTG+AGG - chr8_1:75694286-75694305 Msa1214860:CDS 40.0%
GTTGCAGCTGTTGTAATATG+TGG - chr8_1:75694429-75694448 Msa1214860:CDS 40.0%
!! TTGGTTGGTGGAAGTTTGAT+AGG - chr8_1:75694465-75694484 Msa1214860:CDS 40.0%
AGATCCACAGCTACCATTAT+GGG - chr8_1:75694960-75694979 Msa1214860:intron 40.0%
AAAGACAAATGCCCCCATAA+TGG + chr8_1:75694976-75694995 None:intergenic 40.0%
!! ACTGCTTTTCTTGCTTTGCT+AGG - chr8_1:75695271-75695290 Msa1214860:intron 40.0%
! GCTAGGTTATAGTTTGTAGG+AGG - chr8_1:75695288-75695307 Msa1214860:intron 40.0%
CATCTTTCCGCTAGTATGTT+CGG - chr8_1:75695651-75695670 Msa1214860:intron 40.0%
!!! GCACTTATTTTGAGACCCTT+TGG + chr8_1:75695813-75695832 None:intergenic 40.0%
AAGTGCTCACTTGTACTTCA+TGG - chr8_1:75695827-75695846 Msa1214860:intron 40.0%
GCAACATAGGAGGAAAACTA+TGG + chr8_1:75695856-75695875 None:intergenic 40.0%
TGATGATACTGCTGCATGAT+AGG + chr8_1:75695909-75695928 None:intergenic 40.0%
GTATCATCAGAGTGTACTTC+AGG - chr8_1:75695920-75695939 Msa1214860:intron 40.0%
GTAACTCTATCTATCTTCCC+AGG - chr8_1:75696029-75696048 Msa1214860:intron 40.0%
TGCTGAAGATCTTGAATCCA+AGG - chr8_1:75696058-75696077 Msa1214860:CDS 40.0%
TCTTGAATCCAAGGCTCTTA+AGG - chr8_1:75696067-75696086 Msa1214860:CDS 40.0%
AATCCAAGGCTCTTAAGGAT+TGG - chr8_1:75696072-75696091 Msa1214860:CDS 40.0%
ATACCAATCCTTAAGAGCCT+TGG + chr8_1:75696078-75696097 None:intergenic 40.0%
! ATGGCCCTTTTGATACATTG+TGG + chr8_1:75696170-75696189 None:intergenic 40.0%
CAATGTATCAAAAGGGCCAT+AGG - chr8_1:75696170-75696189 Msa1214860:CDS 40.0%
!! TCTGAGAAATGCACTGACTT+AGG + chr8_1:75696347-75696366 None:intergenic 40.0%
! TGTGAAGGCTTGTTTAGTTC+AGG - chr8_1:75696450-75696469 Msa1214860:CDS 40.0%
TGTCCACACTTCTCATGATT+TGG + chr8_1:75696568-75696587 None:intergenic 40.0%
! TGAGAAGTGTGGACATTGTT+AGG - chr8_1:75696573-75696592 Msa1214860:five_prime_UTR 40.0%
! TAAGCGTCTCTTGTTTCGCT+CGG + chr8_1:75694084-75694103 None:intergenic 45.0%
ATCCATTTCTTGCTTGGAGC+AGG - chr8_1:75694120-75694139 Msa1214860:three_prime_UTR 45.0%
TTCCTGCTCCAAGCAAGAAA+TGG + chr8_1:75694125-75694144 None:intergenic 45.0%
GCCGTGATAAATGCATTCCA+AGG + chr8_1:75694156-75694175 None:intergenic 45.0%
CAGCGAAAACCTTCTCAATG+TGG + chr8_1:75694212-75694231 None:intergenic 45.0%
! CATTGAGAAGGTTTTCGCTG+TGG - chr8_1:75694212-75694231 Msa1214860:CDS 45.0%
! GGTTTTCGCTGTGGCTTATA+TGG - chr8_1:75694221-75694240 Msa1214860:CDS 45.0%
CTTCTTGCGATGATGAGTTG+GGG - chr8_1:75694261-75694280 Msa1214860:CDS 45.0%
CTGAGGTTGCGAATGAACTT+AGG - chr8_1:75694303-75694322 Msa1214860:CDS 45.0%
TGGTAGCTCCTGTCATTGAT+TGG - chr8_1:75694352-75694371 Msa1214860:CDS 45.0%
! GGTAGCTCCTGTCATTGATT+GGG - chr8_1:75694353-75694372 Msa1214860:CDS 45.0%
ATGTGGTTTAGCAGATGGCT+TGG - chr8_1:75694446-75694465 Msa1214860:CDS 45.0%
GGAAGTTTGATAGGATCAGC+AGG - chr8_1:75694474-75694493 Msa1214860:CDS 45.0%
GTTTGATAGGATCAGCAGGA+AGG - chr8_1:75694478-75694497 Msa1214860:CDS 45.0%
CAGATCCACAGCTACCATTA+TGG - chr8_1:75694959-75694978 Msa1214860:intron 45.0%
GATCCACAGCTACCATTATG+GGG - chr8_1:75694961-75694980 Msa1214860:intron 45.0%
ATCCACAGCTACCATTATGG+GGG - chr8_1:75694962-75694981 Msa1214860:intron 45.0%
! GGTTTGAGGTAGTGATGCTT+TGG + chr8_1:75695790-75695809 None:intergenic 45.0%
ATCACTACCTCAAACCCCAA+AGG - chr8_1:75695794-75695813 Msa1214860:intron 45.0%
TCACTACCTCAAACCCCAAA+GGG - chr8_1:75695795-75695814 Msa1214860:intron 45.0%
TGCTCACTTGTACTTCATGG+TGG - chr8_1:75695830-75695849 Msa1214860:intron 45.0%
AGGAGAGTCCCTTATGTTCA+GGG - chr8_1:75695940-75695959 Msa1214860:intron 45.0%
!! AGGGACAAAGTTTTGTGCAG+TGG - chr8_1:75695959-75695978 Msa1214860:intron 45.0%
!! ACAAAGTTTTGTGCAGTGGC+AGG - chr8_1:75695963-75695982 Msa1214860:intron 45.0%
!! CAAAGTTTTGTGCAGTGGCA+GGG - chr8_1:75695964-75695983 Msa1214860:intron 45.0%
TGCAGTGGCAGGGAAAATTA+AGG - chr8_1:75695974-75695993 Msa1214860:intron 45.0%
GCAGTGGCAGGGAAAATTAA+GGG - chr8_1:75695975-75695994 Msa1214860:intron 45.0%
ACAGTATCCAGAACCAACCT+AGG + chr8_1:75696433-75696452 None:intergenic 45.0%
!! GTTGGTTCTGGATACTGTGA+AGG - chr8_1:75696435-75696454 Msa1214860:CDS 45.0%
GTGCCAAATCATGAGAAGTG+TGG - chr8_1:75696562-75696581 Msa1214860:five_prime_UTR 45.0%
!! GAGCAGGAAACTTGCTACCT+TGG - chr8_1:75694136-75694155 Msa1214860:exon 50.0%
! TCATTGATTGGGCCTCGAGT+AGG - chr8_1:75694364-75694383 Msa1214860:CDS 50.0%
! CATTGATTGGGCCTCGAGTA+GGG - chr8_1:75694365-75694384 Msa1214860:CDS 50.0%
TTTCATCTCGTCCCTACTCG+AGG + chr8_1:75694379-75694398 None:intergenic 50.0%
GTAGGGACGAGATGAAAGAG+AGG - chr8_1:75694382-75694401 Msa1214860:CDS 50.0%
GAGATGAAAGAGAGGCCAAG+TGG - chr8_1:75694390-75694409 Msa1214860:CDS 50.0%
GTCATGCCATAAGCACCACT+TGG + chr8_1:75694408-75694427 None:intergenic 50.0%
!! GGTTTAGCAGATGGCTTGGT+TGG - chr8_1:75694450-75694469 Msa1214860:CDS 50.0%
!! TTAGCAGATGGCTTGGTTGG+TGG - chr8_1:75694453-75694472 Msa1214860:CDS 50.0%
TTTGCTGGAACTGCTTCCTC+AGG - chr8_1:75694528-75694547 Msa1214860:CDS 50.0%
!! TTGAGACCCTTTGGGGTTTG+AGG + chr8_1:75695804-75695823 None:intergenic 50.0%
CAGGAGAGTCCCTTATGTTC+AGG - chr8_1:75695939-75695958 Msa1214860:intron 50.0%
ATTGTGATGACCGCGTTCCT+CGG - chr8_1:75696383-75696402 Msa1214860:CDS 50.0%
!! TTGGTTGGTGGTTCTGTCCT+AGG - chr8_1:75696413-75696432 Msa1214860:CDS 50.0%
! TTGGTGGTTCTGTCCTAGGT+TGG - chr8_1:75696417-75696436 Msa1214860:CDS 50.0%
!! GTTCTGTCCTAGGTTGGTTC+TGG - chr8_1:75696423-75696442 Msa1214860:CDS 50.0%
! CTTGCGATGATGAGTTGGGG+AGG - chr8_1:75694264-75694283 Msa1214860:CDS 55.0%
! CGATGATGAGTTGGGGAGGT+TGG - chr8_1:75694268-75694287 Msa1214860:CDS 55.0%
CTCGAGGCCCAATCAATGAC+AGG + chr8_1:75694363-75694382 None:intergenic 55.0%
GAGAGGCCAAGTGGTGCTTA+TGG - chr8_1:75694399-75694418 Msa1214860:CDS 55.0%
CGCGCTATAGCTCCTACCTA+AGG + chr8_1:75694639-75694658 None:intergenic 55.0%
TGCCCCCATAATGGTAGCTG+TGG + chr8_1:75694967-75694986 None:intergenic 55.0%
! TCTTGCTGTGGCAGCACCTA+TGG + chr8_1:75696189-75696208 None:intergenic 55.0%
ATGACCGCGTTCCTCGGATT+CGG - chr8_1:75696389-75696408 Msa1214860:CDS 55.0%
!! TTCCTCGGATTCGGCTTGGT+TGG - chr8_1:75696398-75696417 Msa1214860:CDS 55.0%
CAAGCCGAATCCGAGGAACG+CGG + chr8_1:75696396-75696415 None:intergenic 60.0%
CACCAACCAAGCCGAATCCG+AGG + chr8_1:75696403-75696422 None:intergenic 60.0%
!! CTCGGATTCGGCTTGGTTGG+TGG - chr8_1:75696401-75696420 Msa1214860:CDS 60.0%
CGCGTTCCTCGGATTCGGCT+TGG - chr8_1:75696394-75696413 Msa1214860:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 75693864 75696732 75693864 ID=Msa1214860;Name=Msa1214860
chr8_1 mRNA 75693864 75696732 75693864 ID=Msa1214860-mRNA-1;Parent=Msa1214860;Name=Msa1214860-mRNA-1;_AED=0.01;_eAED=0.01;_QI=187|1|1|1|1|1|2|278|398
chr8_1 exon 75693864 75694839 75693864 ID=Msa1214860-mRNA-1:exon:25996;Parent=Msa1214860-mRNA-1
chr8_1 exon 75696047 75696732 75696047 ID=Msa1214860-mRNA-1:exon:25995;Parent=Msa1214860-mRNA-1
chr8_1 five_prime_UTR 75696546 75696732 75696546 ID=Msa1214860-mRNA-1:five_prime_utr;Parent=Msa1214860-mRNA-1
chr8_1 CDS 75696047 75696545 75696047 ID=Msa1214860-mRNA-1:cds;Parent=Msa1214860-mRNA-1
chr8_1 CDS 75694142 75694839 75694142 ID=Msa1214860-mRNA-1:cds;Parent=Msa1214860-mRNA-1
chr8_1 three_prime_UTR 75693864 75694141 75693864 ID=Msa1214860-mRNA-1:three_prime_utr;Parent=Msa1214860-mRNA-1
Gene Sequence

>Msa1214860

ATGGAAGCTGTTAAGCTAGCATCAAGTGATCCGAGCGAAACAAGAGACGCTTACAAAGTTGCTTACATAATCCATTTCTTGCTTGGAGCAGGAAACTTGCTACCTTGGAATGCATTTATCACGGCAGTTGATTACTTTGCGTACTTATATCCAACTAACCACATTGAGAAGGTTTTCGCTGTGGCTTATATGGTTTCATCTGTGCTTGTACTTCTTGCGATGATGAGTTGGGGAGGTTGGAGTAAAACAACACTGAGGTTGCGAATGAACTTAGGATTTTCAATGTTTGTTATGTCTCTCATGGTAGCTCCTGTCATTGATTGGGCCTCGAGTAGGGACGAGATGAAAGAGAGGCCAAGTGGTGCTTATGGCATGACAGTTGCAGCTGTTGTAATATGTGGTTTAGCAGATGGCTTGGTTGGTGGAAGTTTGATAGGATCAGCAGGAAGGCTTCCAAAACAGTATATGCAAGCTGTTTTTGCTGGAACTGCTTCCTCAGGTATTATAATTTCAATCTTGAGGATAATAACCAAAGCATCACTACCTCAAACCCCAAAGGGTCTCAAAATAAGTGCTCACTTGTACTTCATGGTGGCCATAGTTTTCCTCCTATGTTGCATTGTCTTTAGCAACTTGCAGCACAAGTTACCTATCATGCAGCAGTATCATCAGAGTGTACTTCAGGAGAGTCCCTTATGTTCAGGGACAAAGTTTTGTGCAGTGGCAGGGAAAATTAAGGGACCAGCTTTTGGAATATTCATAATCTATATAGTAACTCTATCTATCTTCCCAGGATTTATTGCTGAAGATCTTGAATCCAAGGCTCTTAAGGATTGGTATCCTATTATATTGATTACAGTTTATAATTTAGCTGATCTTATGGGGAAATCTTTAACTGCATTCTATGTTCCACAATGTATCAAAAGGGCCATAGGTGCTGCCACAGCAAGACTATTATTCTATCCACTTTTTATAGTTTGTCTTCATGGACCAAATTGGTTGAAAACAGAAGTGCCAATGATTGTTCTTACATTTCTGCTTGGATTTACCAATGGATATCTTACTAGTGTTCTTATGATTCTAACTCCTAAGTCAGTGCATTTCTCAGAATCAGAGTTATCTGCTATTGTGATGACCGCGTTCCTCGGATTCGGCTTGGTTGGTGGTTCTGTCCTAGGTTGGTTCTGGATACTGTGA
Protein sequence

>Msa1214860

MEAVKLASSDPSETRDAYKVAYIIHFLLGAGNLLPWNAFITAVDYFAYLYPTNHIEKVFAVAYMVSSVLVLLAMMSWGGWSKTTLRLRMNLGFSMFVMSLMVAPVIDWASSRDEMKERPSGAYGMTVAAVVICGLADGLVGGSLIGSAGRLPKQYMQAVFAGTASSGIIISILRIITKASLPQTPKGLKISAHLYFMVAIVFLLCCIVFSNLQHKLPIMQQYHQSVLQESPLCSGTKFCAVAGKIKGPAFGIFIIYIVTLSIFPGFIAEDLESKALKDWYPIILITVYNLADLMGKSLTAFYVPQCIKRAIGAATARLLFYPLFIVCLHGPNWLKTEVPMIVLTFLLGFTNGYLTSVLMILTPKSVHFSESELSAIVMTAFLGFGLVGGSVLGWFWIL*