AlfalfaGEDB | Msa0269970

M. sativa cultivar zhongmu-4 / Msa0269970

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0269970	RHN80910.1	70.500	400	80	5	26	405	27	408	1.02e-100	315

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0269970	A0A396JRJ4	70.500	400	80	5	26	405	27	408	4.89e-101	315

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000050	Msa0269970	0.805826	1.106462e-49	-8.615850e-47
Msa0036790	Msa0269970	0.847029	1.497413e-59	-8.615850e-47
Msa0081020	Msa0269970	0.940872	1.107314e-100	-8.615850e-47
Msa0099700	Msa0269970	0.800642	1.309476e-48	-8.615850e-47
Msa0115790	Msa0269970	0.802726	4.892516e-49	-8.615850e-47
Msa0132000	Msa0269970	0.890821	6.961658e-74	-8.615850e-47
Msa0132630	Msa0269970	-0.815529	8.825170e-52	-8.615850e-47
Msa0173690	Msa0269970	0.800496	1.402840e-48	-8.615850e-47
Msa0206450	Msa0269970	0.847968	8.262482e-60	-8.615850e-47
Msa0231200	Msa0269970	0.831389	1.716311e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0269970	MtrunA17_Chr1g0193341	70.500	400	80	5	26	405	27	408	9.42e-105	315

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 168 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGACTTGGATATTGAGA+CGG	0.635667	2_3:-1247880	Msa0269980:CDS
AAAAGTTGTTGTTGCTGCAG+AGG	0.578943	2_3:-1247487	Msa0269980:CDS
AAACCTGTCCTCAGAGCCAA+AGG	0.538748	2_3:-1247025	Msa0269980:CDS
AAAGACAACAGCAGCGATAT+CGG	0.428235	2_3:-1249167	Msa0269980:CDS
AAAGCAAAGAAGGCTATCAC+TGG	0.552054	2_3:-1247066	Msa0269980:CDS
AAAGCCCGAGCTTGAGCCTC+TGG	0.433641	2_3:-1249308	Msa0269980:CDS
AACAACATTCAACGGAACAA+AGG	0.561333	2_3:-1248199	Msa0269980:CDS
AACACCAAGAGAAGAACATG+CGG	0.701473	2_3:-1248975	Msa0269980:CDS
AACAGAGAAAGTAGAGCCTG+TGG	0.651574	2_3:-1248030	Msa0269980:CDS
AACAGTAAAAGAAGAACACG+TGG	0.707095	2_3:-1248435	Msa0269980:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGACATTAAGGTCAAATAAT+GGG	-	chr2_3:1247004-1247023	None:intergenic	30.0%
ATCTAAATTCGAAAAAGACG+AGG	-	chr2_3:1247283-1247302	Msa0269980:CDS	30.0%
GAAAAAAGTGAAGGATCTAA+TGG	-	chr2_3:1247702-1247721	Msa0269980:CDS	30.0%
CTAAGTGAACAATTACAAGT+AGG	-	chr2_3:1247774-1247793	Msa0269980:CDS	30.0%
AAAAGACTTGGATATTGAGA+CGG	-	chr2_3:1247883-1247902	Msa0269980:CDS	30.0%
ACTGAAAATCAAGAACAAAC+AGG	-	chr2_3:1247930-1247949	Msa0269980:CDS	30.0%
TGTTTGAATGATTGAATTCC+TGG	+	chr2_3:1247978-1247997	Msa0269970:five_prime_UTR	30.0%
TCAACAATTGCAATTGCATT+TGG	+	chr2_3:1249214-1249233	Msa0269970:CDS	30.0%
ATCACAAGAAATTTCACAAG+AGG	-	chr2_3:1249248-1249267	Msa0269980:CDS	30.0%
GGGACATTAAGGTCAAATAA+TGG	-	chr2_3:1247005-1247024	Msa0269980:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	1246727	1249390	1246727	ID=Msa0269970;Name=Msa0269970
chr2_3	mRNA	1246727	1249390	1246727	ID=Msa0269970-mRNA-1;Parent=Msa0269970;Name=Msa0269970-mRNA-1;_AED=0.15;_eAED=0.47;_QI=1410\|0\|0.5\|1\|0\|0\|2\|0\|405
chr2_3	exon	1246727	1248305	1246727	ID=Msa0269970-mRNA-1:exon:522;Parent=Msa0269970-mRNA-1
chr2_3	exon	1248342	1249390	1248342	ID=Msa0269970-mRNA-1:exon:523;Parent=Msa0269970-mRNA-1
chr2_3	five_prime_UTR	1246727	1248136	1246727	ID=Msa0269970-mRNA-1:five_prime_utr;Parent=Msa0269970-mRNA-1
chr2_3	CDS	1248137	1248305	1248137	ID=Msa0269970-mRNA-1:cds;Parent=Msa0269970-mRNA-1
chr2_3	CDS	1248342	1249390	1248342	ID=Msa0269970-mRNA-1:cds;Parent=Msa0269970-mRNA-1

Gene Sequence

>Msa0269970

ATGACATCATTGGTTTCTGTAGACTGTTCTGGAATTGAAGATGTGTTTGGTTGCTCCTCTTCCCTTTGTTCCGTTGAATGTTGTTCTGGTTCTTTCCGGTTTTCCTCAACTTCAGTAACCACAGGTTCACCTTTTACTGTTTCTGTAGGCTCAACTTCAGTAGCCACAGTAGCCACGGCTTCTTCTTTTACCGTTTCTGTAGGATCAACTTCAGTAACCACAGGTTCGACTTTTACTGTTTCTGTTGGATCAACTTCAGTAACCACGTGTTCTTCTTTTACTGTTTCTGTAGCTTCAACTTCAGTAGCCACGGCTTCTTCTTTTACCGTTTCTGTAGGATCAACTTCAGTAACTACAGGTTCGTCTTTTACCGTTTCTGTAGGCTCAACTACAGTTGCCACAGGTTCTTCTTTTACCGTTTCTGTAGGATCAACTTCAGTAACCACAGGTTCGACTTTTACTGTTTCTGTAGCTTCAACTTCAGTAGCTACAGCTTCTTCTTTTACCGTTTCTGTAGCTTCAACTTCAGCAGCCACAGGTTCTTCTTTTACCGTTTCTGTAGCTTCAACTTCAGCAGCCACAGGTTCGTCTTTTACAGTTTCTGTAGGCTCAACTACAGTAACTGCAGGTTCGTCTTTTACCTTTTCTGTAGGCTCAACTTCAGTTGCCACTGGTTCTTCTTTTACTGTTTCTGTAGGATCAACTTCAGTAACCACAGGTTCGTCTTTTACTGTTTCTGGAACCTCAACTTCAGTAGCTGCAGGTTCTTCTTTTACTGTTTCCGTAGCCTCAACTTCAGTAGCCGCATGTTCTTCTCTTGGTGTTTCTGTAGCCTCAATTTCAGTAACCACGGGTTCTTTGTTTACTATATCTGCAGTCTCAACTTCGGTAACCACGGGTTCTTCTTTTACTTTATCTGCAGTCTCAACTTCGGTAACCACGGGTTCTTCTTTCACTGTTTCGGTAGGCTCTACTTCGATGTTTTTGGTCTCTTCCGATATCGCTGCTGTTGTCTTTTCCTCATTGGCCTCGACTGAAGATTCAACAATTGCAATTGCATTTGGTTGCTCTGCCTCTTGTGAAATTTCTTGTGATTCTTTTTCCGGTTCTTTGGTTATTTTCGGCTCTGCTGAGGCCAGAGGCTCAAGCTCGGGCTTTTCTTCCTTTTCGGCACTTGTTTCTGAAATTTCAGCTTGTTTCACCTCCTCTTCAAATTGA

Protein sequence

>Msa0269970

MTSLVSVDCSGIEDVFGCSSSLCSVECCSGSFRFSSTSVTTGSPFTVSVGSTSVATVATASSFTVSVGSTSVTTGSTFTVSVGSTSVTTCSSFTVSVASTSVATASSFTVSVGSTSVTTGSSFTVSVGSTTVATGSSFTVSVGSTSVTTGSTFTVSVASTSVATASSFTVSVASTSAATGSSFTVSVASTSAATGSSFTVSVGSTTVTAGSSFTFSVGSTSVATGSSFTVSVGSTSVTTGSSFTVSGTSTSVAAGSSFTVSVASTSVAACSSLGVSVASISVTTGSLFTISAVSTSVTTGSSFTLSAVSTSVTTGSSFTVSVGSTSMFLVSSDIAAVVFSSLASTEDSTIAIAFGCSASCEISCDSFSGSLVIFGSAEARGSSSGFSSFSALVSEISACFTSSSN*