AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1332650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1332650 MtrunA17_Chr2g0286471 76.259 139 32 1 1 138 1 139 5.15e-68 202
Msa1332650 MtrunA17_Chr2g0286481 78.862 123 26 0 16 138 17 139 5.54e-65 194
Msa1332650 MtrunA17_Chr8g0385881 48.252 143 63 5 1 137 1 138 8.09e-34 115
Msa1332650 MtrunA17_Chr1g0191251 65.385 78 22 2 57 132 12 86 1.91e-24 90.1
Msa1332650 MtrunA17_Chr4g0066661 85.106 47 7 0 93 139 25 71 4.54e-20 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1332650 AT4G25050.1 53.957 139 58 1 1 139 1 133 2.41e-40 132
Msa1332650 AT4G25050.2 49.669 151 58 2 1 139 1 145 8.29e-37 123
Msa1332650 AT1G54630.1 53.237 139 60 3 1 138 1 135 1.23e-36 122
Msa1332650 AT1G54580.1 54.783 115 44 1 24 138 29 135 2.63e-35 119
Msa1332650 AT5G27200.1 50.000 138 54 4 3 139 16 139 1.10e-34 117
Msa1332650 AT3G05020.1 48.889 135 66 2 6 137 2 136 1.01e-33 115
Msa1332650 AT1G54630.2 48.485 99 46 3 1 98 1 95 5.24e-17 71.6

Find 58 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGAAAGGGAAACTGATTT+TGG 0.126186 8_4:-52585389 None:intergenic
ATCAGTTTCCCTTTCAATTA+AGG 0.135610 8_4:+52585394 Msa1332650:CDS
AGCTCCAAGTGCAGAAAATT+TGG 0.215999 8_4:-52586205 None:intergenic
CAATCATGTCTGCTGCTTCT+TGG 0.254760 8_4:-52586966 None:intergenic
ATGGGACTTGAGGAGGAATT+TGG 0.301410 8_4:+52586901 Msa1332650:CDS
TAGCGAAAAGCTTGTTTGTT+AGG 0.311442 8_4:-52585062 None:intergenic
ATGCATGTGTGTCCTTGTTC+CGG 0.332953 8_4:-52587026 None:intergenic
TCAGTTTCCCTTTCAATTAA+GGG 0.363477 8_4:+52585395 Msa1332650:CDS
CATCCATTACATTGCAGTCT+AGG 0.375528 8_4:+52585429 Msa1332650:CDS
TGCATGTGTGTCCTTGTTCC+GGG 0.389703 8_4:-52587025 None:intergenic
CTCAGCATTCAAACAATCTC+TGG 0.432166 8_4:+52585225 Msa1332650:CDS
AACTACTAAACCCTTCCTTT+CGG 0.433570 8_4:-52584965 None:intergenic
GTTTGTTAGGTGTGTATAGT+TGG 0.443283 8_4:-52585049 None:intergenic
CCAGATGAGTCCTCTGTCAC+TGG 0.444364 8_4:+52586177 Msa1332650:CDS
GGCATTCCCCTTAATTGAAA+GGG 0.445874 8_4:-52585402 None:intergenic
CTTGGACGGTGGTAATGGAC+TGG 0.447324 8_4:-52586948 None:intergenic
TGCTTCTTGGACGGTGGTAA+TGG 0.455810 8_4:-52586953 None:intergenic
ATCCATTACATTGCAGTCTA+GGG 0.489304 8_4:+52585430 Msa1332650:CDS
TACCCTAGACTGCAATGTAA+TGG 0.496902 8_4:-52585432 None:intergenic
CTGTAGGTTGAGATTGTGAT+GGG 0.514505 8_4:+52586883 Msa1332650:intron
AGGCATTCCCCTTAATTGAA+AGG 0.514759 8_4:-52585403 None:intergenic
CCAGTGACAGAGGACTCATC+TGG 0.516812 8_4:-52586177 None:intergenic
CATGTCTGCTGCTTCTTGGA+CGG 0.518674 8_4:-52586962 None:intergenic
CAGTTTCCCTTTCAATTAAG+GGG 0.519673 8_4:+52585396 Msa1332650:CDS
TACACAATGGCATCCATCAC+AGG 0.524640 8_4:+52585187 Msa1332650:exon
GAATGCTGAGACGGAGAATG+AGG 0.525070 8_4:-52585212 None:intergenic
CTGCACACCAACCGAAAGGA+AGG 0.526948 8_4:+52584954 Msa1332650:five_prime_UTR
CTGCAATGTAATGGATGGAA+AGG 0.533896 8_4:-52585423 None:intergenic
GAAAGCAGAAACTGTAAAGA+AGG 0.538034 8_4:+52586122 Msa1332650:CDS
CTAGACTGCAATGTAATGGA+TGG 0.538900 8_4:-52585428 None:intergenic
ACGAAGAGAATGTGTGTTGT+TGG 0.544811 8_4:-52585112 None:intergenic
ACTAAACCCTTCCTTTCGGT+TGG 0.555055 8_4:-52584961 None:intergenic
GAATGAGTAATAACAAGTGT+GGG 0.563297 8_4:-52585090 None:intergenic
AGATTGTTTGAATGCTGAGA+CGG 0.570818 8_4:-52585221 None:intergenic
GGGTTAGAGAATCTGGCACC+AGG 0.571901 8_4:-52585368 None:intergenic
CCTGTAGGTTGAGATTGTGA+TGG 0.575547 8_4:+52586882 Msa1332650:intron
TGGAGCTGACTCTCTCGATA+CGG 0.589016 8_4:+52586221 Msa1332650:CDS
GGAGAATGAGGTTCCTGTGA+TGG 0.592118 8_4:-52585200 None:intergenic
GTCTGCTGCTTCTTGGACGG+TGG 0.592160 8_4:-52586959 None:intergenic
TATGATGTCTATTACGCGGA+GGG 0.592746 8_4:+52587299 Msa1332650:three_prime_UTR
GGAATGAGTAATAACAAGTG+TGG 0.595734 8_4:-52585091 None:intergenic
TGAGATTGTGATGGGACTTG+AGG 0.596018 8_4:+52586891 Msa1332650:CDS
TTGGACGGTGGTAATGGACT+GGG 0.607190 8_4:-52586947 None:intergenic
GATTGTGATGGGACTTGAGG+AGG 0.609784 8_4:+52586894 Msa1332650:CDS
TAGTAATGCAATAATTCTAG+AGG 0.611124 8_4:-52587148 None:intergenic
TTAAGTTCAAACCCGGAACA+AGG 0.611469 8_4:+52587014 Msa1332650:exon
AACACTGCACACCAACCGAA+AGG 0.615132 8_4:+52584950 Msa1332650:five_prime_UTR
ACATCATATCCAAGCCTCAA+AGG 0.619608 8_4:-52587284 None:intergenic
CGATCTTCGAAAGTAACGAA+GGG 0.620036 8_4:+52585022 Msa1332650:five_prime_UTR
ATTCACATCTAACAGGTACC+TGG 0.634844 8_4:+52585350 Msa1332650:intron
ACGATCTTCGAAAGTAACGA+AGG 0.640576 8_4:+52585021 Msa1332650:five_prime_UTR
TTTGGACTCTCCAGTGACAG+AGG 0.640603 8_4:-52586187 None:intergenic
TGCACACCAACCGAAAGGAA+GGG 0.667836 8_4:+52584955 Msa1332650:five_prime_UTR
ATATGATGTCTATTACGCGG+AGG 0.673719 8_4:+52587298 Msa1332650:three_prime_UTR
GCACATGTAACTTGAAAGCG+AGG 0.680439 8_4:-52585455 None:intergenic
AGAGTGCTTAAGTTCAAACC+CGG 0.684631 8_4:+52587007 Msa1332650:exon
CTATCTCTCTCTCTACACAA+TGG 0.684680 8_4:+52585174 Msa1332650:exon
TGGATATGATGTCTATTACG+CGG 0.703548 8_4:+52587295 Msa1332650:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTAATAGTAAAAATCCA+CGG - chr8_4:52586097-52586116 None:intergenic 15.0%
!!! ATTTTAAATATGAAACAAAC+AGG - chr8_4:52586494-52586513 None:intergenic 15.0%
!!! ATTATGTTTTTTAATCTACA+TGG + chr8_4:52586683-52586702 Msa1332650:intron 15.0%
!!! GGTTTTTTTAATTTTATCTT+AGG + chr8_4:52587076-52587095 Msa1332650:three_prime_UTR 15.0%
!! ATATTAATAGTAAAAATCCA+CGG - chr8_4:52586097-52586116 None:intergenic 15.0%
!!! ATTTTAAATATGAAACAAAC+AGG - chr8_4:52586494-52586513 None:intergenic 15.0%
!!! ATTATGTTTTTTAATCTACA+TGG + chr8_4:52586683-52586702 Msa1332650:intron 15.0%
!!! GGTTTTTTTAATTTTATCTT+AGG + chr8_4:52587076-52587095 Msa1332650:three_prime_UTR 15.0%
!!! GAATTTTATGGTTTAATTTG+TGG + chr8_4:52585299-52585318 Msa1332650:intron 20.0%
!!! AATTTTAGTCTATACATACA+TGG - chr8_4:52585633-52585652 None:intergenic 20.0%
!! AATATTACGTACATGTTATA+AGG + chr8_4:52585661-52585680 Msa1332650:intron 20.0%
!!! AAATTTGTTCACATTGAAAT+TGG - chr8_4:52585688-52585707 None:intergenic 20.0%
!! TTGAACAATATATTTGATGT+TGG + chr8_4:52585982-52586001 Msa1332650:intron 20.0%
!!! GGATTTTTACTATTAATATC+AGG + chr8_4:52586098-52586117 Msa1332650:intron 20.0%
!! AAAAAGATGTTATTCAGAAT+TGG - chr8_4:52586554-52586573 None:intergenic 20.0%
!! ATGATATTCTAAATAGAGAA+GGG - chr8_4:52586657-52586676 None:intergenic 20.0%
!! AATGATATTCTAAATAGAGA+AGG - chr8_4:52586658-52586677 None:intergenic 20.0%
!!! AGTTTTTATATTTTTGTGTG+AGG + chr8_4:52587054-52587073 Msa1332650:three_prime_UTR 20.0%
!!! GTTTTTATATTTTTGTGTGA+GGG + chr8_4:52587055-52587074 Msa1332650:three_prime_UTR 20.0%
!!! TTTTATTTATGTAGAACTTG+TGG + chr8_4:52587172-52587191 Msa1332650:three_prime_UTR 20.0%
!!! GAATTTTATGGTTTAATTTG+TGG + chr8_4:52585299-52585318 Msa1332650:intron 20.0%
!!! AATTTTAGTCTATACATACA+TGG - chr8_4:52585633-52585652 None:intergenic 20.0%
!! AATATTACGTACATGTTATA+AGG + chr8_4:52585661-52585680 Msa1332650:intron 20.0%
!!! AAATTTGTTCACATTGAAAT+TGG - chr8_4:52585688-52585707 None:intergenic 20.0%
!! TTGAACAATATATTTGATGT+TGG + chr8_4:52585982-52586001 Msa1332650:intron 20.0%
!!! GGATTTTTACTATTAATATC+AGG + chr8_4:52586098-52586117 Msa1332650:intron 20.0%
!! AAAAAGATGTTATTCAGAAT+TGG - chr8_4:52586554-52586573 None:intergenic 20.0%
!! ATGATATTCTAAATAGAGAA+GGG - chr8_4:52586657-52586676 None:intergenic 20.0%
!! AATGATATTCTAAATAGAGA+AGG - chr8_4:52586658-52586677 None:intergenic 20.0%
!!! AGTTTTTATATTTTTGTGTG+AGG + chr8_4:52587054-52587073 Msa1332650:three_prime_UTR 20.0%
!!! GTTTTTATATTTTTGTGTGA+GGG + chr8_4:52587055-52587074 Msa1332650:three_prime_UTR 20.0%
!!! TTTTATTTATGTAGAACTTG+TGG + chr8_4:52587172-52587191 Msa1332650:three_prime_UTR 20.0%
! TGGAATAAAACAAAACATCA+TGG - chr8_4:52585613-52585632 None:intergenic 25.0%
! AGTTGTGTATTTGAATTGTT+TGG + chr8_4:52585750-52585769 Msa1332650:intron 25.0%
! GTTGTGTATTTGAATTGTTT+GGG + chr8_4:52585751-52585770 Msa1332650:intron 25.0%
! ATTTGATGTTGGAATTATCA+AGG + chr8_4:52585993-52586012 Msa1332650:intron 25.0%
! TGACATTGTCAAAAAACAAT+TGG + chr8_4:52586149-52586168 Msa1332650:CDS 25.0%
! AATAACAACCTAATGAGAAA+TGG - chr8_4:52586463-52586482 None:intergenic 25.0%
! TTAAAATCAGAAGACAGTTA+TGG + chr8_4:52586507-52586526 Msa1332650:intron 25.0%
!! TCTGAATAACATCTTTTTCT+TGG + chr8_4:52586556-52586575 Msa1332650:intron 25.0%
! TAGTAATGCAATAATTCTAG+AGG - chr8_4:52587151-52587170 None:intergenic 25.0%
!! AAGAAAAAGCTTTGTAATAG+AGG - chr8_4:52587248-52587267 None:intergenic 25.0%
!!! TTTTCTTCCATTCTTTTTCA+AGG + chr8_4:52587261-52587280 Msa1332650:three_prime_UTR 25.0%
! TGGAATAAAACAAAACATCA+TGG - chr8_4:52585613-52585632 None:intergenic 25.0%
! AGTTGTGTATTTGAATTGTT+TGG + chr8_4:52585750-52585769 Msa1332650:intron 25.0%
! GTTGTGTATTTGAATTGTTT+GGG + chr8_4:52585751-52585770 Msa1332650:intron 25.0%
! ATTTGATGTTGGAATTATCA+AGG + chr8_4:52585993-52586012 Msa1332650:intron 25.0%
! TGACATTGTCAAAAAACAAT+TGG + chr8_4:52586149-52586168 Msa1332650:CDS 25.0%
! AATAACAACCTAATGAGAAA+TGG - chr8_4:52586463-52586482 None:intergenic 25.0%
! TTAAAATCAGAAGACAGTTA+TGG + chr8_4:52586507-52586526 Msa1332650:intron 25.0%
!! TCTGAATAACATCTTTTTCT+TGG + chr8_4:52586556-52586575 Msa1332650:intron 25.0%
! TAGTAATGCAATAATTCTAG+AGG - chr8_4:52587151-52587170 None:intergenic 25.0%
!! AAGAAAAAGCTTTGTAATAG+AGG - chr8_4:52587248-52587267 None:intergenic 25.0%
!!! TTTTCTTCCATTCTTTTTCA+AGG + chr8_4:52587261-52587280 Msa1332650:three_prime_UTR 25.0%
GAATGAGTAATAACAAGTGT+GGG - chr8_4:52585093-52585112 None:intergenic 30.0%
TGAGTTAATTCGATCAAAGA+AGG - chr8_4:52585273-52585292 None:intergenic 30.0%
! AACTCATCACTGGAATTTTA+TGG + chr8_4:52585287-52585306 Msa1332650:intron 30.0%
TATGCTAATTCACATCTAAC+AGG + chr8_4:52585343-52585362 Msa1332650:intron 30.0%
!! TTGAAAGGGAAACTGATTTT+GGG - chr8_4:52585391-52585410 None:intergenic 30.0%
!! ATTGAAAGGGAAACTGATTT+TGG - chr8_4:52585392-52585411 None:intergenic 30.0%
ATCAGTTTCCCTTTCAATTA+AGG + chr8_4:52585394-52585413 Msa1332650:CDS 30.0%
TCAGTTTCCCTTTCAATTAA+GGG + chr8_4:52585395-52585414 Msa1332650:CDS 30.0%
!! TTTTTGCTTTTGTTCATTGC+TGG - chr8_4:52585730-52585749 None:intergenic 30.0%
CTCAAAAACCATTTCTCATT+AGG + chr8_4:52586452-52586471 Msa1332650:intron 30.0%
! TGTCATTTAGTATATTGCTG+TGG + chr8_4:52586587-52586606 Msa1332650:intron 30.0%
CTAGAAAAGATACATAACAG+AGG - chr8_4:52586833-52586852 None:intergenic 30.0%
GAATGAGTAATAACAAGTGT+GGG - chr8_4:52585093-52585112 None:intergenic 30.0%
TGAGTTAATTCGATCAAAGA+AGG - chr8_4:52585273-52585292 None:intergenic 30.0%
! AACTCATCACTGGAATTTTA+TGG + chr8_4:52585287-52585306 Msa1332650:intron 30.0%
TATGCTAATTCACATCTAAC+AGG + chr8_4:52585343-52585362 Msa1332650:intron 30.0%
!! TTGAAAGGGAAACTGATTTT+GGG - chr8_4:52585391-52585410 None:intergenic 30.0%
!! ATTGAAAGGGAAACTGATTT+TGG - chr8_4:52585392-52585411 None:intergenic 30.0%
ATCAGTTTCCCTTTCAATTA+AGG + chr8_4:52585394-52585413 Msa1332650:CDS 30.0%
TCAGTTTCCCTTTCAATTAA+GGG + chr8_4:52585395-52585414 Msa1332650:CDS 30.0%
!! TTTTTGCTTTTGTTCATTGC+TGG - chr8_4:52585730-52585749 None:intergenic 30.0%
CTCAAAAACCATTTCTCATT+AGG + chr8_4:52586452-52586471 Msa1332650:intron 30.0%
! TGTCATTTAGTATATTGCTG+TGG + chr8_4:52586587-52586606 Msa1332650:intron 30.0%
CTAGAAAAGATACATAACAG+AGG - chr8_4:52586833-52586852 None:intergenic 30.0%
AACTACTAAACCCTTCCTTT+CGG - chr8_4:52584968-52584987 None:intergenic 35.0%
! GTTTGTTAGGTGTGTATAGT+TGG - chr8_4:52585052-52585071 None:intergenic 35.0%
! TAGCGAAAAGCTTGTTTGTT+AGG - chr8_4:52585065-52585084 None:intergenic 35.0%
GGAATGAGTAATAACAAGTG+TGG - chr8_4:52585094-52585113 None:intergenic 35.0%
AGATTGTTTGAATGCTGAGA+CGG - chr8_4:52585224-52585243 None:intergenic 35.0%
TGATCGAATTAACTCATCAC+TGG + chr8_4:52585277-52585296 Msa1332650:intron 35.0%
!!! TGATTTTGGGTTAGAGAATC+TGG - chr8_4:52585378-52585397 None:intergenic 35.0%
CAGTTTCCCTTTCAATTAAG+GGG + chr8_4:52585396-52585415 Msa1332650:CDS 35.0%
ATCCATTACATTGCAGTCTA+GGG + chr8_4:52585430-52585449 Msa1332650:CDS 35.0%
AGGGAGTAAAAGAGTTTAGA+GGG - chr8_4:52585498-52585517 None:intergenic 35.0%
TAGGGAGTAAAAGAGTTTAG+AGG - chr8_4:52585499-52585518 None:intergenic 35.0%
AAATGTTGCTAGTTCAGAAG+AGG + chr8_4:52585853-52585872 Msa1332650:intron 35.0%
CACACAGAATCAAATATCAG+AGG - chr8_4:52586029-52586048 None:intergenic 35.0%
CTCTGATATTTGATTCTGTG+TGG + chr8_4:52586027-52586046 Msa1332650:intron 35.0%
GAAAGCAGAAACTGTAAAGA+AGG + chr8_4:52586122-52586141 Msa1332650:CDS 35.0%
TATTGCCTTATCTAAGTGTG+TGG + chr8_4:52586352-52586371 Msa1332650:intron 35.0%
TCATGTACTTCTTGCTTTCT+GGG + chr8_4:52586375-52586394 Msa1332650:intron 35.0%
AGACTTGTCAAATTTCCTGT+AGG + chr8_4:52586867-52586886 Msa1332650:intron 35.0%
TCAAAGGCCTTGAAAAAGAA+TGG - chr8_4:52587271-52587290 None:intergenic 35.0%
!!! ATTCTTTTTCAAGGCCTTTG+AGG + chr8_4:52587270-52587289 Msa1332650:three_prime_UTR 35.0%
TGGATATGATGTCTATTACG+CGG + chr8_4:52587295-52587314 Msa1332650:three_prime_UTR 35.0%
AACTACTAAACCCTTCCTTT+CGG - chr8_4:52584968-52584987 None:intergenic 35.0%
! GTTTGTTAGGTGTGTATAGT+TGG - chr8_4:52585052-52585071 None:intergenic 35.0%
! TAGCGAAAAGCTTGTTTGTT+AGG - chr8_4:52585065-52585084 None:intergenic 35.0%
GGAATGAGTAATAACAAGTG+TGG - chr8_4:52585094-52585113 None:intergenic 35.0%
AGATTGTTTGAATGCTGAGA+CGG - chr8_4:52585224-52585243 None:intergenic 35.0%
TGATCGAATTAACTCATCAC+TGG + chr8_4:52585277-52585296 Msa1332650:intron 35.0%
!!! TGATTTTGGGTTAGAGAATC+TGG - chr8_4:52585378-52585397 None:intergenic 35.0%
CAGTTTCCCTTTCAATTAAG+GGG + chr8_4:52585396-52585415 Msa1332650:CDS 35.0%
ATCCATTACATTGCAGTCTA+GGG + chr8_4:52585430-52585449 Msa1332650:CDS 35.0%
AGGGAGTAAAAGAGTTTAGA+GGG - chr8_4:52585498-52585517 None:intergenic 35.0%
TAGGGAGTAAAAGAGTTTAG+AGG - chr8_4:52585499-52585518 None:intergenic 35.0%
AAATGTTGCTAGTTCAGAAG+AGG + chr8_4:52585853-52585872 Msa1332650:intron 35.0%
CACACAGAATCAAATATCAG+AGG - chr8_4:52586029-52586048 None:intergenic 35.0%
CTCTGATATTTGATTCTGTG+TGG + chr8_4:52586027-52586046 Msa1332650:intron 35.0%
GAAAGCAGAAACTGTAAAGA+AGG + chr8_4:52586122-52586141 Msa1332650:CDS 35.0%
TATTGCCTTATCTAAGTGTG+TGG + chr8_4:52586352-52586371 Msa1332650:intron 35.0%
TCATGTACTTCTTGCTTTCT+GGG + chr8_4:52586375-52586394 Msa1332650:intron 35.0%
AGACTTGTCAAATTTCCTGT+AGG + chr8_4:52586867-52586886 Msa1332650:intron 35.0%
TCAAAGGCCTTGAAAAAGAA+TGG - chr8_4:52587271-52587290 None:intergenic 35.0%
!!! ATTCTTTTTCAAGGCCTTTG+AGG + chr8_4:52587270-52587289 Msa1332650:three_prime_UTR 35.0%
TGGATATGATGTCTATTACG+CGG + chr8_4:52587295-52587314 Msa1332650:three_prime_UTR 35.0%
ACGATCTTCGAAAGTAACGA+AGG + chr8_4:52585021-52585040 Msa1332650:five_prime_UTR 40.0%
CGATCTTCGAAAGTAACGAA+GGG + chr8_4:52585022-52585041 Msa1332650:five_prime_UTR 40.0%
ACGAAGAGAATGTGTGTTGT+TGG - chr8_4:52585115-52585134 None:intergenic 40.0%
CTATCTCTCTCTCTACACAA+TGG + chr8_4:52585174-52585193 Msa1332650:exon 40.0%
CTCAGCATTCAAACAATCTC+TGG + chr8_4:52585225-52585244 Msa1332650:CDS 40.0%
ATTCACATCTAACAGGTACC+TGG + chr8_4:52585350-52585369 Msa1332650:intron 40.0%
GGCATTCCCCTTAATTGAAA+GGG - chr8_4:52585405-52585424 None:intergenic 40.0%
AGGCATTCCCCTTAATTGAA+AGG - chr8_4:52585406-52585425 None:intergenic 40.0%
!! CTGCAATGTAATGGATGGAA+AGG - chr8_4:52585426-52585445 None:intergenic 40.0%
! CTAGACTGCAATGTAATGGA+TGG - chr8_4:52585431-52585450 None:intergenic 40.0%
CATCCATTACATTGCAGTCT+AGG + chr8_4:52585429-52585448 Msa1332650:CDS 40.0%
TACCCTAGACTGCAATGTAA+TGG - chr8_4:52585435-52585454 None:intergenic 40.0%
AAGAGTTTAGAGGGTTTCAG+AGG - chr8_4:52585489-52585508 None:intergenic 40.0%
!! AGAGGAGAATGGATTTGTTG+TGG + chr8_4:52585871-52585890 Msa1332650:intron 40.0%
! GAGTCCAAATTTTCTGCACT+TGG + chr8_4:52586201-52586220 Msa1332650:CDS 40.0%
AGCTCCAAGTGCAGAAAATT+TGG - chr8_4:52586208-52586227 None:intergenic 40.0%
CATGACCACACACTTAGATA+AGG - chr8_4:52586360-52586379 None:intergenic 40.0%
GTCATGTACTTCTTGCTTTC+TGG + chr8_4:52586374-52586393 Msa1332650:intron 40.0%
CAGGGTGATAAAAGCAAGAA+GGG - chr8_4:52586737-52586756 None:intergenic 40.0%
TCATGCTGAAGTTTCCACTT+AGG - chr8_4:52586781-52586800 None:intergenic 40.0%
! CTGTAGGTTGAGATTGTGAT+GGG + chr8_4:52586883-52586902 Msa1332650:intron 40.0%
! AGAGTGCTTAAGTTCAAACC+CGG + chr8_4:52587007-52587026 Msa1332650:exon 40.0%
TTAAGTTCAAACCCGGAACA+AGG + chr8_4:52587014-52587033 Msa1332650:exon 40.0%
ACATCATATCCAAGCCTCAA+AGG - chr8_4:52587287-52587306 None:intergenic 40.0%
ATATGATGTCTATTACGCGG+AGG + chr8_4:52587298-52587317 Msa1332650:three_prime_UTR 40.0%
TATGATGTCTATTACGCGGA+GGG + chr8_4:52587299-52587318 Msa1332650:three_prime_UTR 40.0%
ACGATCTTCGAAAGTAACGA+AGG + chr8_4:52585021-52585040 Msa1332650:five_prime_UTR 40.0%
CGATCTTCGAAAGTAACGAA+GGG + chr8_4:52585022-52585041 Msa1332650:five_prime_UTR 40.0%
ACGAAGAGAATGTGTGTTGT+TGG - chr8_4:52585115-52585134 None:intergenic 40.0%
CTATCTCTCTCTCTACACAA+TGG + chr8_4:52585174-52585193 Msa1332650:exon 40.0%
CTCAGCATTCAAACAATCTC+TGG + chr8_4:52585225-52585244 Msa1332650:CDS 40.0%
ATTCACATCTAACAGGTACC+TGG + chr8_4:52585350-52585369 Msa1332650:intron 40.0%
GGCATTCCCCTTAATTGAAA+GGG - chr8_4:52585405-52585424 None:intergenic 40.0%
AGGCATTCCCCTTAATTGAA+AGG - chr8_4:52585406-52585425 None:intergenic 40.0%
!! CTGCAATGTAATGGATGGAA+AGG - chr8_4:52585426-52585445 None:intergenic 40.0%
! CTAGACTGCAATGTAATGGA+TGG - chr8_4:52585431-52585450 None:intergenic 40.0%
CATCCATTACATTGCAGTCT+AGG + chr8_4:52585429-52585448 Msa1332650:CDS 40.0%
TACCCTAGACTGCAATGTAA+TGG - chr8_4:52585435-52585454 None:intergenic 40.0%
AAGAGTTTAGAGGGTTTCAG+AGG - chr8_4:52585489-52585508 None:intergenic 40.0%
!! AGAGGAGAATGGATTTGTTG+TGG + chr8_4:52585871-52585890 Msa1332650:intron 40.0%
! GAGTCCAAATTTTCTGCACT+TGG + chr8_4:52586201-52586220 Msa1332650:CDS 40.0%
AGCTCCAAGTGCAGAAAATT+TGG - chr8_4:52586208-52586227 None:intergenic 40.0%
CATGACCACACACTTAGATA+AGG - chr8_4:52586360-52586379 None:intergenic 40.0%
GTCATGTACTTCTTGCTTTC+TGG + chr8_4:52586374-52586393 Msa1332650:intron 40.0%
CAGGGTGATAAAAGCAAGAA+GGG - chr8_4:52586737-52586756 None:intergenic 40.0%
TCATGCTGAAGTTTCCACTT+AGG - chr8_4:52586781-52586800 None:intergenic 40.0%
! CTGTAGGTTGAGATTGTGAT+GGG + chr8_4:52586883-52586902 Msa1332650:intron 40.0%
! AGAGTGCTTAAGTTCAAACC+CGG + chr8_4:52587007-52587026 Msa1332650:exon 40.0%
TTAAGTTCAAACCCGGAACA+AGG + chr8_4:52587014-52587033 Msa1332650:exon 40.0%
ACATCATATCCAAGCCTCAA+AGG - chr8_4:52587287-52587306 None:intergenic 40.0%
ATATGATGTCTATTACGCGG+AGG + chr8_4:52587298-52587317 Msa1332650:three_prime_UTR 40.0%
ACTAAACCCTTCCTTTCGGT+TGG - chr8_4:52584964-52584983 None:intergenic 45.0%
AAGAGAGAGAGAGAGAGAGA+TGG - chr8_4:52585147-52585166 None:intergenic 45.0%
TACACAATGGCATCCATCAC+AGG + chr8_4:52585187-52585206 Msa1332650:exon 45.0%
GCACATGTAACTTGAAAGCG+AGG - chr8_4:52585458-52585477 None:intergenic 45.0%
GCTAGTTCAGAAGAGGAGAA+TGG + chr8_4:52585860-52585879 Msa1332650:intron 45.0%
CTGTTGTGATATAAACGCCG+TGG + chr8_4:52586077-52586096 Msa1332650:intron 45.0%
GCAGGGTGATAAAAGCAAGA+AGG - chr8_4:52586738-52586757 None:intergenic 45.0%
CTTAAGTGGTTCATGAGGCA+GGG - chr8_4:52586755-52586774 None:intergenic 45.0%
AACCACTTAAGCTGCCTAAG+TGG + chr8_4:52586764-52586783 Msa1332650:intron 45.0%
TTCCACTTAGGCAGCTTAAG+TGG - chr8_4:52586769-52586788 None:intergenic 45.0%
CCATCACAATCTCAACCTAC+AGG - chr8_4:52586885-52586904 None:intergenic 45.0%
! CCTGTAGGTTGAGATTGTGA+TGG + chr8_4:52586882-52586901 Msa1332650:intron 45.0%
! TGAGATTGTGATGGGACTTG+AGG + chr8_4:52586891-52586910 Msa1332650:CDS 45.0%
ATGGGACTTGAGGAGGAATT+TGG + chr8_4:52586901-52586920 Msa1332650:CDS 45.0%
! CAATCATGTCTGCTGCTTCT+TGG - chr8_4:52586969-52586988 None:intergenic 45.0%
ATGCATGTGTGTCCTTGTTC+CGG - chr8_4:52587029-52587048 None:intergenic 45.0%
!!! TTTTCAAGGCCTTTGAGGCT+TGG + chr8_4:52587275-52587294 Msa1332650:three_prime_UTR 45.0%
ACTAAACCCTTCCTTTCGGT+TGG - chr8_4:52584964-52584983 None:intergenic 45.0%
AAGAGAGAGAGAGAGAGAGA+TGG - chr8_4:52585147-52585166 None:intergenic 45.0%
TACACAATGGCATCCATCAC+AGG + chr8_4:52585187-52585206 Msa1332650:exon 45.0%
GCACATGTAACTTGAAAGCG+AGG - chr8_4:52585458-52585477 None:intergenic 45.0%
GCTAGTTCAGAAGAGGAGAA+TGG + chr8_4:52585860-52585879 Msa1332650:intron 45.0%
CTGTTGTGATATAAACGCCG+TGG + chr8_4:52586077-52586096 Msa1332650:intron 45.0%
GCAGGGTGATAAAAGCAAGA+AGG - chr8_4:52586738-52586757 None:intergenic 45.0%
CTTAAGTGGTTCATGAGGCA+GGG - chr8_4:52586755-52586774 None:intergenic 45.0%
AACCACTTAAGCTGCCTAAG+TGG + chr8_4:52586764-52586783 Msa1332650:intron 45.0%
TTCCACTTAGGCAGCTTAAG+TGG - chr8_4:52586769-52586788 None:intergenic 45.0%
CCATCACAATCTCAACCTAC+AGG - chr8_4:52586885-52586904 None:intergenic 45.0%
! CCTGTAGGTTGAGATTGTGA+TGG + chr8_4:52586882-52586901 Msa1332650:intron 45.0%
! TGAGATTGTGATGGGACTTG+AGG + chr8_4:52586891-52586910 Msa1332650:CDS 45.0%
ATGGGACTTGAGGAGGAATT+TGG + chr8_4:52586901-52586920 Msa1332650:CDS 45.0%
! CAATCATGTCTGCTGCTTCT+TGG - chr8_4:52586969-52586988 None:intergenic 45.0%
ATGCATGTGTGTCCTTGTTC+CGG - chr8_4:52587029-52587048 None:intergenic 45.0%
!!! TTTTCAAGGCCTTTGAGGCT+TGG + chr8_4:52587275-52587294 Msa1332650:three_prime_UTR 45.0%
!! TAAAATAAAAAGAAAATATA+GGG - chr8_4:52585517-52585536 None:intergenic 5.0%
!! ATAAAATAAAAAGAAAATAT+AGG - chr8_4:52585518-52585537 None:intergenic 5.0%
!!! TTTTCTTTTTATTTTATTTT+TGG + chr8_4:52585521-52585540 Msa1332650:intron 5.0%
!! TAAAATAAAAAGAAAATATA+GGG - chr8_4:52585517-52585536 None:intergenic 5.0%
!! ATAAAATAAAAAGAAAATAT+AGG - chr8_4:52585518-52585537 None:intergenic 5.0%
!!! TTTTCTTTTTATTTTATTTT+TGG + chr8_4:52585521-52585540 Msa1332650:intron 5.0%
AACACTGCACACCAACCGAA+AGG + chr8_4:52584950-52584969 Msa1332650:five_prime_UTR 50.0%
TGCACACCAACCGAAAGGAA+GGG + chr8_4:52584955-52584974 Msa1332650:five_prime_UTR 50.0%
GAGAGAGAGAGAGAGATGGA+CGG - chr8_4:52585143-52585162 None:intergenic 50.0%
GGAGAATGAGGTTCCTGTGA+TGG - chr8_4:52585203-52585222 None:intergenic 50.0%
GAATGCTGAGACGGAGAATG+AGG - chr8_4:52585215-52585234 None:intergenic 50.0%
TTTGGACTCTCCAGTGACAG+AGG - chr8_4:52586190-52586209 None:intergenic 50.0%
! TGGAGCTGACTCTCTCGATA+CGG + chr8_4:52586221-52586240 Msa1332650:CDS 50.0%
GCTTAAGTGGTTCATGAGGC+AGG - chr8_4:52586756-52586775 None:intergenic 50.0%
GGCAGCTTAAGTGGTTCATG+AGG - chr8_4:52586760-52586779 None:intergenic 50.0%
! GATTGTGATGGGACTTGAGG+AGG + chr8_4:52586894-52586913 Msa1332650:CDS 50.0%
!! TTGGACGGTGGTAATGGACT+GGG - chr8_4:52586950-52586969 None:intergenic 50.0%
TGCTTCTTGGACGGTGGTAA+TGG - chr8_4:52586956-52586975 None:intergenic 50.0%
! CATGTCTGCTGCTTCTTGGA+CGG - chr8_4:52586965-52586984 None:intergenic 50.0%
TGCATGTGTGTCCTTGTTCC+GGG - chr8_4:52587028-52587047 None:intergenic 50.0%
AACACTGCACACCAACCGAA+AGG + chr8_4:52584950-52584969 Msa1332650:five_prime_UTR 50.0%
TGCACACCAACCGAAAGGAA+GGG + chr8_4:52584955-52584974 Msa1332650:five_prime_UTR 50.0%
GAGAGAGAGAGAGAGATGGA+CGG - chr8_4:52585143-52585162 None:intergenic 50.0%
GGAGAATGAGGTTCCTGTGA+TGG - chr8_4:52585203-52585222 None:intergenic 50.0%
GAATGCTGAGACGGAGAATG+AGG - chr8_4:52585215-52585234 None:intergenic 50.0%
TTTGGACTCTCCAGTGACAG+AGG - chr8_4:52586190-52586209 None:intergenic 50.0%
! TGGAGCTGACTCTCTCGATA+CGG + chr8_4:52586221-52586240 Msa1332650:CDS 50.0%
GCTTAAGTGGTTCATGAGGC+AGG - chr8_4:52586756-52586775 None:intergenic 50.0%
GGCAGCTTAAGTGGTTCATG+AGG - chr8_4:52586760-52586779 None:intergenic 50.0%
! GATTGTGATGGGACTTGAGG+AGG + chr8_4:52586894-52586913 Msa1332650:CDS 50.0%
!! TTGGACGGTGGTAATGGACT+GGG - chr8_4:52586950-52586969 None:intergenic 50.0%
TGCTTCTTGGACGGTGGTAA+TGG - chr8_4:52586956-52586975 None:intergenic 50.0%
! CATGTCTGCTGCTTCTTGGA+CGG - chr8_4:52586965-52586984 None:intergenic 50.0%
TGCATGTGTGTCCTTGTTCC+GGG - chr8_4:52587028-52587047 None:intergenic 50.0%
CTGCACACCAACCGAAAGGA+AGG + chr8_4:52584954-52584973 Msa1332650:five_prime_UTR 55.0%
GAGAGAGAGAGATGGACGGA+GGG - chr8_4:52585139-52585158 None:intergenic 55.0%
AGAGAGAGAGAGATGGACGG+AGG - chr8_4:52585140-52585159 None:intergenic 55.0%
GGGTTAGAGAATCTGGCACC+AGG - chr8_4:52585371-52585390 None:intergenic 55.0%
CCAGTGACAGAGGACTCATC+TGG - chr8_4:52586180-52586199 None:intergenic 55.0%
CCAGATGAGTCCTCTGTCAC+TGG + chr8_4:52586177-52586196 Msa1332650:CDS 55.0%
!! CTTGGACGGTGGTAATGGAC+TGG - chr8_4:52586951-52586970 None:intergenic 55.0%
CTGCACACCAACCGAAAGGA+AGG + chr8_4:52584954-52584973 Msa1332650:five_prime_UTR 55.0%
GAGAGAGAGAGATGGACGGA+GGG - chr8_4:52585139-52585158 None:intergenic 55.0%
AGAGAGAGAGAGATGGACGG+AGG - chr8_4:52585140-52585159 None:intergenic 55.0%
GGGTTAGAGAATCTGGCACC+AGG - chr8_4:52585371-52585390 None:intergenic 55.0%
CCAGTGACAGAGGACTCATC+TGG - chr8_4:52586180-52586199 None:intergenic 55.0%
CCAGATGAGTCCTCTGTCAC+TGG + chr8_4:52586177-52586196 Msa1332650:CDS 55.0%
!! CTTGGACGGTGGTAATGGAC+TGG - chr8_4:52586951-52586970 None:intergenic 55.0%
! GTCTGCTGCTTCTTGGACGG+TGG - chr8_4:52586962-52586981 None:intergenic 60.0%
! GTCTGCTGCTTCTTGGACGG+TGG - chr8_4:52586962-52586981 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 52584942 52587321 52584942 ID=Msa1332650;Name=Msa1332650
chr8_4 mRNA 52584942 52587321 52584942 ID=Msa1332650-mRNA-1;Parent=Msa1332650;Name=Msa1332650-mRNA-1;_AED=0.01;_eAED=0.01;_QI=251|1|1|1|1|1|4|304|139
chr8_4 exon 52584942 52585246 52584942 ID=Msa1332650-mRNA-1:exon:15767;Parent=Msa1332650-mRNA-1
chr8_4 exon 52585365 52585478 52585365 ID=Msa1332650-mRNA-1:exon:15768;Parent=Msa1332650-mRNA-1
chr8_4 exon 52586120 52586242 52586120 ID=Msa1332650-mRNA-1:exon:15769;Parent=Msa1332650-mRNA-1
chr8_4 exon 52586889 52587321 52586889 ID=Msa1332650-mRNA-1:exon:15770;Parent=Msa1332650-mRNA-1
chr8_4 five_prime_UTR 52584942 52585192 52584942 ID=Msa1332650-mRNA-1:five_prime_utr;Parent=Msa1332650-mRNA-1
chr8_4 CDS 52585193 52585246 52585193 ID=Msa1332650-mRNA-1:cds;Parent=Msa1332650-mRNA-1
chr8_4 CDS 52585365 52585478 52585365 ID=Msa1332650-mRNA-1:cds;Parent=Msa1332650-mRNA-1
chr8_4 CDS 52586120 52586242 52586120 ID=Msa1332650-mRNA-1:cds;Parent=Msa1332650-mRNA-1
chr8_4 CDS 52586889 52587017 52586889 ID=Msa1332650-mRNA-1:cds;Parent=Msa1332650-mRNA-1
chr8_4 three_prime_UTR 52587018 52587321 52587018 ID=Msa1332650-mRNA-1:three_prime_utr;Parent=Msa1332650-mRNA-1
Gene Sequence

>Msa1332650

ATGGCATCCATCACAGGAACCTCATTCTCCGTCTCAGCATTCAAACAATCTCTGGTACCTGGTGCCAGATTCTCTAACCCAAAATCAGTTTCCCTTTCAATTAAGGGGAATGCCTTTCCATCCATTACATTGCAGTCTAGGGTACCTCGCTTTCAAGTTACATGTGCTGCGAAAGCAGAAACTGTAAAGAAGGTCTGTGACATTGTCAAAAAACAATTGGCATTGCCAGATGAGTCCTCTGTCACTGGAGAGTCCAAATTTTCTGCACTTGGAGCTGACTCTCTCGATACGGTTGAGATTGTGATGGGACTTGAGGAGGAATTTGGTATTAGTGTTGAAGAAGAAAGTGCCCAGTCCATTACCACCGTCCAAGAAGCAGCAGACATGATTGATAATCTTCTTGAAAACAAGAGTGCTTAA
Protein sequence

>Msa1332650

MASITGTSFSVSAFKQSLVPGARFSNPKSVSLSIKGNAFPSITLQSRVPRFQVTCAAKAETVKKVCDIVKKQLALPDESSVTGESKFSALGADSLDTVEIVMGLEEEFGISVEEESAQSITTVQEAADMIDNLLENKSA*