Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1217050 | GAU11100.1 | 95.522 | 67 | 3 | 0 | 1 | 67 | 71 | 137 | 4.08e-35 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1217050 | sp|P93092|ACP1_CASGL | 81.818 | 66 | 12 | 0 | 1 | 66 | 70 | 135 | 6.67e-32 | 110 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000560 | Msa1217050 | 0.834080 | 3.684683e-56 | -8.615850e-47 |
| Msa0025160 | Msa1217050 | 0.809031 | 2.313367e-50 | -8.615850e-47 |
| Msa0035270 | Msa1217050 | 0.834849 | 2.361887e-56 | -8.615850e-47 |
| Msa0035340 | Msa1217050 | 0.817256 | 3.624044e-52 | -8.615850e-47 |
| Msa0606580 | Msa1217050 | 0.812221 | 4.727588e-51 | -8.615850e-47 |
| Msa0607800 | Msa1217050 | 0.802472 | 5.520182e-49 | -8.615850e-47 |
| Msa0647280 | Msa1217050 | 0.814152 | 1.781899e-51 | -8.615850e-47 |
| Msa0652650 | Msa1217050 | 0.817444 | 3.287921e-52 | -8.615850e-47 |
| Msa0686800 | Msa1217050 | 0.812160 | 4.876446e-51 | -8.615850e-47 |
| Msa0694200 | Msa1217050 | 0.804743 | 1.865499e-49 | -8.615850e-47 |
| Msa0730930 | Msa1217050 | 0.817983 | 2.484397e-52 | -8.615850e-47 |
| Msa0735780 | Msa1217050 | 0.804794 | 1.820651e-49 | -8.615850e-47 |
| Msa0747000 | Msa1217050 | 0.801198 | 1.008025e-48 | -8.615850e-47 |
| Msa0780910 | Msa1217050 | 0.803948 | 2.732491e-49 | -8.615850e-47 |
| Msa0821160 | Msa1217050 | 0.800035 | 1.740342e-48 | -8.615850e-47 |
| Msa0129380 | Msa1217050 | 0.823246 | 1.535760e-53 | -8.615850e-47 |
| Msa0146650 | Msa1217050 | 0.802904 | 4.496651e-49 | -8.615850e-47 |
| Msa0176060 | Msa1217050 | 0.821076 | 4.892250e-53 | -8.615850e-47 |
| Msa0367520 | Msa1217050 | 0.806658 | 7.390777e-50 | -8.615850e-47 |
| Msa0468700 | Msa1217050 | 0.814672 | 1.367108e-51 | -8.615850e-47 |
| Msa1035610 | Msa1217050 | 0.806008 | 1.013252e-49 | -8.615850e-47 |
| Msa1042160 | Msa1217050 | 0.807967 | 3.901754e-50 | -8.615850e-47 |
| Msa1042190 | Msa1217050 | 0.810812 | 9.568473e-51 | -8.615850e-47 |
| Msa1104110 | Msa1217050 | 0.854283 | 1.364301e-61 | -8.615850e-47 |
| Msa1130280 | Msa1217050 | 0.836972 | 6.839104e-57 | -8.615850e-47 |
| Msa1132530 | Msa1217050 | 0.801694 | 7.979836e-49 | -8.615850e-47 |
| Msa1147700 | Msa1217050 | 0.837155 | 6.142269e-57 | -8.615850e-47 |
| Msa1167470 | Msa1217050 | 0.811009 | 8.672458e-51 | -8.615850e-47 |
| Msa1217050 | Msa1217210 | 0.870973 | 9.730951e-67 | -8.615850e-47 |
| Msa1217050 | Msa1262590 | 0.918839 | 9.487049e-87 | -8.615850e-47 |
| Msa1217050 | Msa1306570 | 0.830596 | 2.687314e-55 | -8.615850e-47 |
| Msa1217050 | Msa1332650 | 0.886633 | 2.886785e-72 | -8.615850e-47 |
| Msa1217050 | Msa1374790 | 0.805643 | 1.209047e-49 | -8.615850e-47 |
| Msa1217050 | Msa1375350 | 0.833563 | 4.962546e-56 | -8.615850e-47 |
| Msa1217050 | Msa1393780 | 0.808492 | 3.015953e-50 | -8.615850e-47 |
| Msa1217050 | Msa1411790 | 0.822172 | 2.730350e-53 | -8.615850e-47 |
| Msa1217050 | Msa1414170 | 0.824785 | 6.687650e-54 | -8.615850e-47 |
| Msa1217050 | Msa1431480 | 0.815964 | 7.056495e-52 | -8.615850e-47 |
| Msa1217050 | Msa1451820 | 0.808278 | 3.349483e-50 | -8.615850e-47 |
| Msa0514580 | Msa1217050 | 0.803362 | 3.614472e-49 | -8.615850e-47 |
| Msa0517250 | Msa1217050 | 0.824951 | 6.111702e-54 | -8.615850e-47 |
| Msa0556170 | Msa1217050 | 0.801340 | 9.431787e-49 | -8.615850e-47 |
| Msa0591170 | Msa1217050 | 0.823463 | 1.366886e-53 | -8.615850e-47 |
| Msa0945260 | Msa1217050 | 0.802887 | 4.532058e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1217050 | MtrunA17_Chr2g0286471 | 84.848 | 66 | 10 | 0 | 1 | 66 | 74 | 139 | 5.88e-34 | 112 |
| Msa1217050 | MtrunA17_Chr2g0286481 | 84.848 | 66 | 10 | 0 | 1 | 66 | 74 | 139 | 7.48e-34 | 112 |
| Msa1217050 | MtrunA17_Chr8g0385881 | 61.538 | 65 | 25 | 0 | 1 | 65 | 74 | 138 | 1.80e-23 | 85.9 |
| Msa1217050 | MtrunA17_Chr4g0066661 | 85.106 | 47 | 7 | 0 | 21 | 67 | 25 | 71 | 5.25e-21 | 78.6 |
| Msa1217050 | MtrunA17_Chr1g0191251 | 67.241 | 58 | 14 | 2 | 1 | 56 | 28 | 82 | 5.20e-16 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1217050 | AT4G25050.1 | 68.657 | 67 | 21 | 0 | 1 | 67 | 67 | 133 | 1.91e-25 | 91.3 |
| Msa1217050 | AT5G27200.1 | 61.194 | 67 | 26 | 0 | 1 | 67 | 73 | 139 | 1.03e-22 | 84.3 |
| Msa1217050 | AT4G25050.2 | 58.228 | 79 | 21 | 1 | 1 | 67 | 67 | 145 | 4.83e-22 | 82.8 |
| Msa1217050 | AT1G54630.1 | 62.121 | 66 | 25 | 0 | 1 | 66 | 70 | 135 | 2.68e-21 | 80.9 |
| Msa1217050 | AT1G54580.1 | 62.121 | 66 | 25 | 0 | 1 | 66 | 70 | 135 | 2.77e-21 | 80.9 |
| Msa1217050 | AT3G05020.1 | 58.462 | 65 | 27 | 0 | 1 | 65 | 72 | 136 | 1.06e-19 | 76.6 |
Find 16 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTCCAAGTGCAGAAAATT+TGG | 0.215999 | 8_1:+79271665 | None:intergenic |
| CAATCATGTCTGCTGCTTCT+TGG | 0.254760 | 8_1:+79270904 | None:intergenic |
| ATGGGACTTGAGGAGGAATT+TGG | 0.301410 | 8_1:-79270969 | Msa1217050:CDS |
| CCAGATGAGTCCTCTGTCAC+TGG | 0.444364 | 8_1:-79271693 | Msa1217050:CDS |
| CTTGGACGGTGGTAATGGAC+TGG | 0.447324 | 8_1:+79270922 | None:intergenic |
| TGCTTCTTGGACGGTGGTAA+TGG | 0.455810 | 8_1:+79270917 | None:intergenic |
| CTGTAGGTTGAGATTGTGAT+GGG | 0.514505 | 8_1:-79270987 | Msa1217050:intron |
| CCAGTGACAGAGGACTCATC+TGG | 0.516812 | 8_1:+79271693 | None:intergenic |
| CATGTCTGCTGCTTCTTGGA+CGG | 0.518674 | 8_1:+79270908 | None:intergenic |
| CCTGTAGGTTGAGATTGTGA+TGG | 0.575547 | 8_1:-79270988 | Msa1217050:intron |
| TGGAGCTGACTCTCTCGATA+CGG | 0.589016 | 8_1:-79271649 | Msa1217050:intron |
| GTCTGCTGCTTCTTGGACGG+TGG | 0.592160 | 8_1:+79270911 | None:intergenic |
| TGAGATTGTGATGGGACTTG+AGG | 0.596018 | 8_1:-79270979 | Msa1217050:CDS |
| TTGGACGGTGGTAATGGACT+GGG | 0.607190 | 8_1:+79270923 | None:intergenic |
| GATTGTGATGGGACTTGAGG+AGG | 0.609784 | 8_1:-79270976 | Msa1217050:CDS |
| TTTGGACTCTCCAGTGACAG+AGG | 0.640603 | 8_1:+79271683 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTAAATATGAAACAAAC+AGG | + | chr8_1:79271201-79271220 | None:intergenic | 15.0% |
| !!! | ATTATGTTTTTTAATCTACA+TGG | - | chr8_1:79271390-79271409 | Msa1217050:intron | 15.0% |
| !!! | ATTTTAAATATGAAACAAAC+AGG | + | chr8_1:79271201-79271220 | None:intergenic | 15.0% |
| !!! | ATTATGTTTTTTAATCTACA+TGG | - | chr8_1:79271390-79271409 | Msa1217050:intron | 15.0% |
| !! | AAAAAGATGTTATTCAGAAT+TGG | + | chr8_1:79271261-79271280 | None:intergenic | 20.0% |
| !! | ATGATATTCTAAATAGAGAA+GGG | + | chr8_1:79271364-79271383 | None:intergenic | 20.0% |
| !! | AATGATATTCTAAATAGAGA+AGG | + | chr8_1:79271365-79271384 | None:intergenic | 20.0% |
| !! | AAAAAGATGTTATTCAGAAT+TGG | + | chr8_1:79271261-79271280 | None:intergenic | 20.0% |
| !! | ATGATATTCTAAATAGAGAA+GGG | + | chr8_1:79271364-79271383 | None:intergenic | 20.0% |
| !! | AATGATATTCTAAATAGAGA+AGG | + | chr8_1:79271365-79271384 | None:intergenic | 20.0% |
| ! | ACTTAGATAAGGCAATAAAA+TGG | + | chr8_1:79271056-79271075 | None:intergenic | 25.0% |
| ! | AATAACAACCTAATGAGAAA+TGG | + | chr8_1:79271170-79271189 | None:intergenic | 25.0% |
| ! | TTAAAATCAGAAGACAGTTA+TGG | - | chr8_1:79271214-79271233 | Msa1217050:intron | 25.0% |
| !! | TCTGAATAACATCTTTTTCT+TGG | - | chr8_1:79271263-79271282 | Msa1217050:intron | 25.0% |
| ! | ACTTAGATAAGGCAATAAAA+TGG | + | chr8_1:79271056-79271075 | None:intergenic | 25.0% |
| ! | AATAACAACCTAATGAGAAA+TGG | + | chr8_1:79271170-79271189 | None:intergenic | 25.0% |
| ! | TTAAAATCAGAAGACAGTTA+TGG | - | chr8_1:79271214-79271233 | Msa1217050:intron | 25.0% |
| !! | TCTGAATAACATCTTTTTCT+TGG | - | chr8_1:79271263-79271282 | Msa1217050:intron | 25.0% |
| !!! | TAATGCTATCTTGCTGTTTT+TGG | - | chr8_1:79271110-79271129 | Msa1217050:intron | 30.0% |
| CTCAAAAACCATTTCTCATT+AGG | - | chr8_1:79271159-79271178 | Msa1217050:intron | 30.0% | |
| CTAGAAAAGATACATAACAG+AGG | + | chr8_1:79271540-79271559 | None:intergenic | 30.0% | |
| !!! | TAATGCTATCTTGCTGTTTT+TGG | - | chr8_1:79271110-79271129 | Msa1217050:intron | 30.0% |
| CTCAAAAACCATTTCTCATT+AGG | - | chr8_1:79271159-79271178 | Msa1217050:intron | 30.0% | |
| CTAGAAAAGATACATAACAG+AGG | + | chr8_1:79271540-79271559 | None:intergenic | 30.0% | |
| TATTGCCTTATCTAAGTGTG+TGG | - | chr8_1:79271059-79271078 | Msa1217050:intron | 35.0% | |
| TCATGTACTTCTTGCTTTCT+GGG | - | chr8_1:79271082-79271101 | Msa1217050:intron | 35.0% | |
| TGTCCTTTAGTATATTGCTG+TGG | - | chr8_1:79271294-79271313 | Msa1217050:intron | 35.0% | |
| TTGCCACAGCAATATACTAA+AGG | + | chr8_1:79271300-79271319 | None:intergenic | 35.0% | |
| AGACTTGTCAAATTTCCTGT+AGG | - | chr8_1:79271574-79271593 | Msa1217050:intron | 35.0% | |
| TATTGCCTTATCTAAGTGTG+TGG | - | chr8_1:79271059-79271078 | Msa1217050:intron | 35.0% | |
| TCATGTACTTCTTGCTTTCT+GGG | - | chr8_1:79271082-79271101 | Msa1217050:intron | 35.0% | |
| TGTCCTTTAGTATATTGCTG+TGG | - | chr8_1:79271294-79271313 | Msa1217050:intron | 35.0% | |
| TTGCCACAGCAATATACTAA+AGG | + | chr8_1:79271300-79271319 | None:intergenic | 35.0% | |
| AGACTTGTCAAATTTCCTGT+AGG | - | chr8_1:79271574-79271593 | Msa1217050:intron | 35.0% | |
| ! | GAGTCCAAATTTTCTGCACT+TGG | - | chr8_1:79270908-79270927 | Msa1217050:CDS | 40.0% |
| AGCTCCAAGTGCAGAAAATT+TGG | + | chr8_1:79270915-79270934 | None:intergenic | 40.0% | |
| CATGACCACACACTTAGATA+AGG | + | chr8_1:79271067-79271086 | None:intergenic | 40.0% | |
| GTCATGTACTTCTTGCTTTC+TGG | - | chr8_1:79271081-79271100 | Msa1217050:intron | 40.0% | |
| CAGGGTGATAAAAGCAAGAA+GGG | + | chr8_1:79271444-79271463 | None:intergenic | 40.0% | |
| TCATGCTGAAGTTTCCACTT+AGG | + | chr8_1:79271488-79271507 | None:intergenic | 40.0% | |
| ! | CTGTAGGTTGAGATTGTGAT+GGG | - | chr8_1:79271590-79271609 | Msa1217050:intron | 40.0% |
| ! | GAGTCCAAATTTTCTGCACT+TGG | - | chr8_1:79270908-79270927 | Msa1217050:CDS | 40.0% |
| AGCTCCAAGTGCAGAAAATT+TGG | + | chr8_1:79270915-79270934 | None:intergenic | 40.0% | |
| CATGACCACACACTTAGATA+AGG | + | chr8_1:79271067-79271086 | None:intergenic | 40.0% | |
| GTCATGTACTTCTTGCTTTC+TGG | - | chr8_1:79271081-79271100 | Msa1217050:intron | 40.0% | |
| CAGGGTGATAAAAGCAAGAA+GGG | + | chr8_1:79271444-79271463 | None:intergenic | 40.0% | |
| TCATGCTGAAGTTTCCACTT+AGG | + | chr8_1:79271488-79271507 | None:intergenic | 40.0% | |
| ! | CTGTAGGTTGAGATTGTGAT+GGG | - | chr8_1:79271590-79271609 | Msa1217050:intron | 40.0% |
| GCAGGGTGATAAAAGCAAGA+AGG | + | chr8_1:79271445-79271464 | None:intergenic | 45.0% | |
| CTTAAGTGGTTCATGAGGCA+GGG | + | chr8_1:79271462-79271481 | None:intergenic | 45.0% | |
| AACCACTTAAGCTGCCTAAG+TGG | - | chr8_1:79271471-79271490 | Msa1217050:intron | 45.0% | |
| TTCCACTTAGGCAGCTTAAG+TGG | + | chr8_1:79271476-79271495 | None:intergenic | 45.0% | |
| CCATCACAATCTCAACCTAC+AGG | + | chr8_1:79271592-79271611 | None:intergenic | 45.0% | |
| ! | CCTGTAGGTTGAGATTGTGA+TGG | - | chr8_1:79271589-79271608 | Msa1217050:intron | 45.0% |
| ! | TGAGATTGTGATGGGACTTG+AGG | - | chr8_1:79271598-79271617 | Msa1217050:intron | 45.0% |
| ATGGGACTTGAGGAGGAATT+TGG | - | chr8_1:79271608-79271627 | Msa1217050:intron | 45.0% | |
| ! | CAATCATGTCTGCTGCTTCT+TGG | + | chr8_1:79271676-79271695 | None:intergenic | 45.0% |
| GCAGGGTGATAAAAGCAAGA+AGG | + | chr8_1:79271445-79271464 | None:intergenic | 45.0% | |
| CTTAAGTGGTTCATGAGGCA+GGG | + | chr8_1:79271462-79271481 | None:intergenic | 45.0% | |
| AACCACTTAAGCTGCCTAAG+TGG | - | chr8_1:79271471-79271490 | Msa1217050:intron | 45.0% | |
| TTCCACTTAGGCAGCTTAAG+TGG | + | chr8_1:79271476-79271495 | None:intergenic | 45.0% | |
| CCATCACAATCTCAACCTAC+AGG | + | chr8_1:79271592-79271611 | None:intergenic | 45.0% | |
| ! | CCTGTAGGTTGAGATTGTGA+TGG | - | chr8_1:79271589-79271608 | Msa1217050:intron | 45.0% |
| ! | TGAGATTGTGATGGGACTTG+AGG | - | chr8_1:79271598-79271617 | Msa1217050:intron | 45.0% |
| ATGGGACTTGAGGAGGAATT+TGG | - | chr8_1:79271608-79271627 | Msa1217050:intron | 45.0% | |
| ! | CAATCATGTCTGCTGCTTCT+TGG | + | chr8_1:79271676-79271695 | None:intergenic | 45.0% |
| TTTGGACTCTCCAGTGACAG+AGG | + | chr8_1:79270897-79270916 | None:intergenic | 50.0% | |
| ! | TGGAGCTGACTCTCTCGATA+CGG | - | chr8_1:79270928-79270947 | Msa1217050:CDS | 50.0% |
| GCTTAAGTGGTTCATGAGGC+AGG | + | chr8_1:79271463-79271482 | None:intergenic | 50.0% | |
| GGCAGCTTAAGTGGTTCATG+AGG | + | chr8_1:79271467-79271486 | None:intergenic | 50.0% | |
| ! | GATTGTGATGGGACTTGAGG+AGG | - | chr8_1:79271601-79271620 | Msa1217050:intron | 50.0% |
| !! | TTGGACGGTGGTAATGGACT+GGG | + | chr8_1:79271657-79271676 | None:intergenic | 50.0% |
| TGCTTCTTGGACGGTGGTAA+TGG | + | chr8_1:79271663-79271682 | None:intergenic | 50.0% | |
| ! | CATGTCTGCTGCTTCTTGGA+CGG | + | chr8_1:79271672-79271691 | None:intergenic | 50.0% |
| TTTGGACTCTCCAGTGACAG+AGG | + | chr8_1:79270897-79270916 | None:intergenic | 50.0% | |
| ! | TGGAGCTGACTCTCTCGATA+CGG | - | chr8_1:79270928-79270947 | Msa1217050:CDS | 50.0% |
| GCTTAAGTGGTTCATGAGGC+AGG | + | chr8_1:79271463-79271482 | None:intergenic | 50.0% | |
| GGCAGCTTAAGTGGTTCATG+AGG | + | chr8_1:79271467-79271486 | None:intergenic | 50.0% | |
| ! | GATTGTGATGGGACTTGAGG+AGG | - | chr8_1:79271601-79271620 | Msa1217050:intron | 50.0% |
| !! | TTGGACGGTGGTAATGGACT+GGG | + | chr8_1:79271657-79271676 | None:intergenic | 50.0% |
| TGCTTCTTGGACGGTGGTAA+TGG | + | chr8_1:79271663-79271682 | None:intergenic | 50.0% | |
| ! | CATGTCTGCTGCTTCTTGGA+CGG | + | chr8_1:79271672-79271691 | None:intergenic | 50.0% |
| CCAGTGACAGAGGACTCATC+TGG | + | chr8_1:79270887-79270906 | None:intergenic | 55.0% | |
| CCAGATGAGTCCTCTGTCAC+TGG | - | chr8_1:79270884-79270903 | Msa1217050:CDS | 55.0% | |
| !! | CTTGGACGGTGGTAATGGAC+TGG | + | chr8_1:79271658-79271677 | None:intergenic | 55.0% |
| CCAGTGACAGAGGACTCATC+TGG | + | chr8_1:79270887-79270906 | None:intergenic | 55.0% | |
| CCAGATGAGTCCTCTGTCAC+TGG | - | chr8_1:79270884-79270903 | Msa1217050:CDS | 55.0% | |
| !! | CTTGGACGGTGGTAATGGAC+TGG | + | chr8_1:79271658-79271677 | None:intergenic | 55.0% |
| ! | GTCTGCTGCTTCTTGGACGG+TGG | + | chr8_1:79271669-79271688 | None:intergenic | 60.0% |
| ! | GTCTGCTGCTTCTTGGACGG+TGG | + | chr8_1:79271669-79271688 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 79270875 | 79271724 | 79270875 | ID=Msa1217050;Name=Msa1217050 |
| chr8_1 | mRNA | 79270875 | 79271724 | 79270875 | ID=Msa1217050-mRNA-1;Parent=Msa1217050;Name=Msa1217050-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0|0|1|1|1|2|0|67 |
| chr8_1 | exon | 79271650 | 79271724 | 79271650 | ID=Msa1217050-mRNA-1:exon:27550;Parent=Msa1217050-mRNA-1 |
| chr8_1 | exon | 79270875 | 79271003 | 79270875 | ID=Msa1217050-mRNA-1:exon:27549;Parent=Msa1217050-mRNA-1 |
| chr8_1 | CDS | 79271650 | 79271724 | 79271650 | ID=Msa1217050-mRNA-1:cds;Parent=Msa1217050-mRNA-1 |
| chr8_1 | CDS | 79270875 | 79271003 | 79270875 | ID=Msa1217050-mRNA-1:cds;Parent=Msa1217050-mRNA-1 |
| Gene Sequence |
| Protein sequence |
