AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1332840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1332840 MtrunA17_Chr4g0068671 95.021 241 12 0 1 241 1 241 2.40e-139 390
Msa1332840 MtrunA17_Chr2g0294131 55.285 246 81 7 1 241 1 222 1.63e-53 172
Msa1332840 MtrunA17_Chr1g0194401 40.120 167 95 4 67 232 25 187 2.78e-28 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1332840 AT1G02380.1 34.649 228 124 10 3 225 2 209 5.59e-28 107
Msa1332840 AT3G17120.4 43.478 161 57 6 71 208 30 179 2.05e-26 102
Msa1332840 AT3G17120.2 43.478 161 57 6 71 208 30 179 2.05e-26 102
Msa1332840 AT3G17120.1 43.478 161 57 6 71 208 30 179 2.05e-26 102
Msa1332840 AT3G17120.5 43.478 161 57 6 71 208 30 179 2.05e-26 102
Msa1332840 AT3G17120.3 43.478 161 57 6 71 208 30 179 2.05e-26 102
Msa1332840 AT4G01960.1 50.495 101 47 3 113 213 106 203 2.12e-23 95.1
Msa1332840 AT4G01960.2 50.495 101 47 3 113 213 106 203 2.12e-23 95.1
Msa1332840 AT4G01960.3 65.152 66 22 1 113 178 106 170 2.40e-20 85.9

Find 82 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTTTACATTGTGATTTA+AGG 0.098041 8_4:-52793626 Msa1332840:three_prime_UTR
CTGCTGATCAAAAGCTAATT+AGG 0.140099 8_4:-52794744 Msa1332840:CDS
TTAACCTAAACTACAAAATT+AGG 0.172537 8_4:+52795237 None:intergenic
TTTGTCCCCTCATCGGATCT+TGG 0.258976 8_4:-52795784 Msa1332840:five_prime_UTR
TGTAGTTTAGGTTAACAATC+AGG 0.318334 8_4:-52795229 Msa1332840:five_prime_UTR
GACATTGATATAAAATAATT+AGG 0.333160 8_4:+52793516 None:intergenic
GAGTTCAGTGACAAGTTTCC+AGG 0.338873 8_4:-52794024 Msa1332840:CDS
GACATTGATATAAAATAATT+AGG 0.339980 8_4:+52793477 None:intergenic
TTGTCCCCTCATCGGATCTT+GGG 0.368020 8_4:-52795783 Msa1332840:five_prime_UTR
GGCAGTAACTAAAGATGATT+CGG 0.374127 8_4:-52795043 Msa1332840:CDS
GTCTAAACTTCACCCTCATC+AGG 0.389569 8_4:+52793586 None:intergenic
TGTATTGTGATCTTATTTCA+AGG 0.410724 8_4:-52794589 Msa1332840:intron
GAGAGAGATTTGAATGCAAC+AGG 0.411193 8_4:-52795866 Msa1332840:five_prime_UTR
AGGGTGAAGTTTAGACTTGT+AGG 0.415952 8_4:-52793579 Msa1332840:three_prime_UTR
TGTAGAGAATAGTCCGGTTA+AGG 0.419669 8_4:-52794839 Msa1332840:CDS
TGCTGATCAAAAGCTAATTA+GGG 0.424444 8_4:-52794743 Msa1332840:CDS
CAAAACTAATAATTAAACTT+TGG 0.429207 8_4:+52795132 None:intergenic
AAGATTTATTGTGGTATTGT+TGG 0.429970 8_4:-52794988 Msa1332840:CDS
TACATTGTGATTTAAGGTGT+TGG 0.433731 8_4:-52793620 Msa1332840:three_prime_UTR
TTTGCAGGCATGTCATCATC+TGG 0.437634 8_4:-52794050 Msa1332840:intron
ACATTGTGATTTAAGGTGTT+GGG 0.440893 8_4:-52793619 Msa1332840:three_prime_UTR
TCAGCTCCTCCTGCAGATAC+AGG 0.451996 8_4:+52793996 None:intergenic
CTAAACTACAAAATTAGGTT+AGG 0.452713 8_4:+52795242 None:intergenic
CAGGGGTCTCCTGTATCTGC+AGG 0.453931 8_4:-52794005 Msa1332840:CDS
GCAGTAACTAAAGATGATTC+GGG 0.461926 8_4:-52795042 Msa1332840:CDS
GGTGAAGTTGATATTGAAAG+TGG 0.477042 8_4:-52795069 Msa1332840:CDS
ATGGCAAACATGCTGCTGCT+TGG 0.493125 8_4:+52794631 None:intergenic
AGATTTACTGACAATAAAGA+TGG 0.495380 8_4:-52793701 Msa1332840:three_prime_UTR
TATGCTCTTTGTCCCCTCAT+CGG 0.500303 8_4:-52795791 Msa1332840:five_prime_UTR
GTTGTTGACGGGGCAGGACT+TGG 0.513107 8_4:-52794869 Msa1332840:CDS
GAGTCTAACAAGTTGTTGAC+GGG 0.513280 8_4:-52794880 Msa1332840:CDS
AGCTCCACGAGGGCCGTCGT+TGG 0.517134 8_4:-52794770 Msa1332840:CDS
GTAGTTTAGGTTAACAATCA+GGG 0.520089 8_4:-52795228 Msa1332840:five_prime_UTR
CACCCTCGCACGCTTCAACA+TGG 0.521089 8_4:+52794705 None:intergenic
ATAGTCCGGTTAAGGAGAAA+CGG 0.534537 8_4:-52794831 Msa1332840:CDS
TTGAAAGATTCAAATTTGAG+TGG 0.539571 8_4:-52794925 Msa1332840:CDS
AAGCAGCAGCATGTTTGCCA+TGG 0.544835 8_4:-52794629 Msa1332840:CDS
TAACAAGTTGTTGACGGGGC+AGG 0.545014 8_4:-52794875 Msa1332840:CDS
AATAATTAGGTGATTACAGA+TGG 0.545580 8_4:+52793529 None:intergenic
TTATTTGCTAAGATTTATTG+TGG 0.547930 8_4:-52794997 Msa1332840:CDS
CGGGTAATACTAAACAAGAA+AGG 0.549432 8_4:-52795023 Msa1332840:CDS
GACGGCCCTCGTGGAGCTCT+AGG 0.551220 8_4:+52794775 None:intergenic
GGAGAGAGAAGATGCTAGGA+AGG 0.551589 8_4:+52795948 None:intergenic
AGAAAACTGAGATGAGATGA+AGG 0.556919 8_4:-52793731 Msa1332840:exon
TAAATGTTCCTCTCACAAAG+AGG 0.559209 8_4:+52795190 None:intergenic
GGGTAATTTATCCCCTGATG+AGG 0.559462 8_4:-52793599 Msa1332840:three_prime_UTR
CACAATGTAAAGAAATACAT+TGG 0.560595 8_4:+52793634 None:intergenic
CATGTTGAAGCGTGCGAGGG+TGG 0.561704 8_4:-52794704 Msa1332840:CDS
TTAGGTTAACAATCAGGGTG+AGG 0.567911 8_4:-52795223 Msa1332840:five_prime_UTR
AGAGTCTAACAAGTTGTTGA+CGG 0.570293 8_4:-52794881 Msa1332840:CDS
GAAAAGTGTAGAGAATAGTC+CGG 0.572405 8_4:-52794845 Msa1332840:CDS
GTGAAGTTGATATTGAAAGT+GGG 0.572872 8_4:-52795068 Msa1332840:CDS
GGTAATTTATCCCCTGATGA+GGG 0.577082 8_4:-52793598 Msa1332840:three_prime_UTR
TCTAAACTTCACCCTCATCA+GGG 0.581373 8_4:+52793587 None:intergenic
AGAAGAGAACCACCGACAGT+TGG 0.582996 8_4:+52795973 None:intergenic
AGTTCAGTGACAAGTTTCCA+GGG 0.583334 8_4:-52794023 Msa1332840:CDS
GCATCCAACGACGGCCCTCG+TGG 0.589028 8_4:+52794766 None:intergenic
CTGCAGATACAGGAGACCCC+TGG 0.589034 8_4:+52794006 None:intergenic
TACTTGTGAGACTCTGAAGA+AGG 0.591671 8_4:+52793909 None:intergenic
GGGTCTCCTGTATCTGCAGG+AGG 0.593983 8_4:-52794002 Msa1332840:CDS
AGCTGGAGAGAGAAGATGCT+AGG 0.600356 8_4:+52795944 None:intergenic
TTGCCATGTTGAAGCGTGCG+AGG 0.607905 8_4:-52794708 Msa1332840:CDS
AGAGACGGACACTCACTCAC+AGG 0.610518 8_4:+52795814 None:intergenic
AGAAACCCAAGATCCGATGA+GGG 0.614811 8_4:+52795778 None:intergenic
AGTTGATATTGAAAGTGGGT+TGG 0.627313 8_4:-52795064 Msa1332840:CDS
AAGAAACCCAAGATCCGATG+AGG 0.644513 8_4:+52795777 None:intergenic
CTACCTTACAGAATTGTACG+TGG 0.646628 8_4:-52793767 Msa1332840:intron
GAGTGGAGATTCTGTGAATG+TGG 0.653592 8_4:-52794908 Msa1332840:CDS
TGATCAGCAGCATCCAACGA+CGG 0.653843 8_4:+52794757 None:intergenic
TGCCATGTTGAAGCGTGCGA+GGG 0.655794 8_4:-52794707 Msa1332840:CDS
CTAAACTTCACCCTCATCAG+GGG 0.659411 8_4:+52793588 None:intergenic
GGCCCTCGTGGAGCTCTAGG+AGG 0.662072 8_4:+52794778 None:intergenic
GGGCTTGTGTGAGATGCACA+AGG 0.666533 8_4:+52793670 None:intergenic
GCAGCAGTGTTGCCAACTGT+CGG 0.673083 8_4:-52795985 Msa1332840:five_prime_UTR
GAAACCCAAGATCCGATGAG+GGG 0.673655 8_4:+52795779 None:intergenic
GGGACAAAGAGCATAAGAGA+CGG 0.674570 8_4:+52795799 None:intergenic
GTTCAGTGACAAGTTTCCAG+GGG 0.676920 8_4:-52794022 Msa1332840:CDS
GAAGACAACAGTGCAAACCA+TGG 0.690412 8_4:+52794612 None:intergenic
AGTCTAACAAGTTGTTGACG+GGG 0.706378 8_4:-52794879 Msa1332840:CDS
GCAGTGTTGCCAACTGTCGG+TGG 0.713536 8_4:-52795982 Msa1332840:five_prime_UTR
AACCTCCTAGAGCTCCACGA+GGG 0.737863 8_4:-52794780 Msa1332840:CDS
AAACCTCCTAGAGCTCCACG+AGG 0.781040 8_4:-52794781 Msa1332840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATAATAATAAATTTTAA+TGG + chr8_4:52795169-52795188 None:intergenic 0.0%
!!! ATTAAAATTTATTATTATTT+TGG - chr8_4:52795168-52795187 Msa1332840:five_prime_UTR 0.0%
!!! TATTTTTTTTCTACAATTTC+AGG - chr8_4:52794069-52794088 Msa1332840:intron 15.0%
!!! ATTTTTTTTCTACAATTTCA+GGG - chr8_4:52794070-52794089 Msa1332840:intron 15.0%
!! CAAAACTAATAATTAAACTT+TGG + chr8_4:52794337-52794356 None:intergenic 15.0%
!! GACATTGATATAAAATAATT+AGG + chr8_4:52795953-52795972 None:intergenic 15.0%
!! GACATTGATATAAAATAATT+AGG + chr8_4:52795953-52795972 None:intergenic 15.0%
!!! GTTTTATTGTAAATGAAAGA+AGG + chr8_4:52793733-52793752 None:intergenic 20.0%
!! AAAAAATGAATGACATTGAA+GGG + chr8_4:52793788-52793807 None:intergenic 20.0%
!! TATTGCTATGTTTAATTCAT+TGG - chr8_4:52793860-52793879 Msa1332840:intron 20.0%
!!! TGTCTGATATCATTTAAAAT+TGG + chr8_4:52793945-52793964 None:intergenic 20.0%
!!! TTTTTTTTCTACAATTTCAG+GGG - chr8_4:52794071-52794090 Msa1332840:intron 20.0%
!! AATTGCAAATGACAAAAAAA+TGG + chr8_4:52794156-52794175 None:intergenic 20.0%
!! TTAACCTAAACTACAAAATT+AGG + chr8_4:52794232-52794251 None:intergenic 20.0%
!!! AATTATTAGTTTTGTGAGTT+TGG - chr8_4:52794343-52794362 Msa1332840:intron 20.0%
!!! ATTATTAGTTTTGTGAGTTT+GGG - chr8_4:52794344-52794363 Msa1332840:intron 20.0%
!! ATGGATGATAAAAATATGAA+AGG - chr8_4:52794367-52794386 Msa1332840:intron 20.0%
!! TGGATGATAAAAATATGAAA+GGG - chr8_4:52794368-52794387 Msa1332840:intron 20.0%
!!! TTATTTGCTAAGATTTATTG+TGG - chr8_4:52794469-52794488 Msa1332840:intron 20.0%
!! ACAATTGACAAATGATATAA+TGG - chr8_4:52794993-52795012 Msa1332840:CDS 20.0%
!! TACTCAATCAATATGAAATT+CGG + chr8_4:52795238-52795257 None:intergenic 20.0%
!!! GACTTGAAATTTTATTCAAT+TGG + chr8_4:52795526-52795545 None:intergenic 20.0%
!! AACAATAAGAATATTACTAC+CGG + chr8_4:52795671-52795690 None:intergenic 20.0%
!! TTTCTTTACATTGTGATTTA+AGG - chr8_4:52795840-52795859 Msa1332840:five_prime_UTR 20.0%
! AAAAATGGGATTTATATAGC+TGG + chr8_4:52793542-52793561 None:intergenic 25.0%
! TCTGTGATTCTTCAAAAAAT+GGG + chr8_4:52793556-52793575 None:intergenic 25.0%
! ATCTGTGATTCTTCAAAAAA+TGG + chr8_4:52793557-52793576 None:intergenic 25.0%
! ATGAATGAAAGAAAGAAAGA+GGG + chr8_4:52793756-52793775 None:intergenic 25.0%
! AATGAATGAAAGAAAGAAAG+AGG + chr8_4:52793757-52793776 None:intergenic 25.0%
! GAAAAAATGAATGACATTGA+AGG + chr8_4:52793789-52793808 None:intergenic 25.0%
! TTAAATGATATCAGACAACT+CGG - chr8_4:52793948-52793967 Msa1332840:CDS 25.0%
!!! TTTTTTTCTACAATTTCAGG+GGG - chr8_4:52794072-52794091 Msa1332840:intron 25.0%
!! AATGGTTTTCTACAAATTGA+GGG + chr8_4:52794138-52794157 None:intergenic 25.0%
!! AAATGGTTTTCTACAAATTG+AGG + chr8_4:52794139-52794158 None:intergenic 25.0%
! CTAAACTACAAAATTAGGTT+AGG + chr8_4:52794227-52794246 None:intergenic 25.0%
!! CTAACCTAATTTTGTAGTTT+AGG - chr8_4:52794225-52794244 Msa1332840:intron 25.0%
! GGATGATAAAAATATGAAAG+GGG - chr8_4:52794369-52794388 Msa1332840:intron 25.0%
! AAGATTTATTGTGGTATTGT+TGG - chr8_4:52794478-52794497 Msa1332840:intron 25.0%
! TTGAAAGATTCAAATTTGAG+TGG - chr8_4:52794541-52794560 Msa1332840:intron 25.0%
! GAAAAAATCTGGTAACAAAA+AGG - chr8_4:52794657-52794676 Msa1332840:CDS 25.0%
!! TGTATTGTGATCTTATTTCA+AGG - chr8_4:52794877-52794896 Msa1332840:CDS 25.0%
! AATTAAAGTAGTATCTGTCA+TGG - chr8_4:52794910-52794929 Msa1332840:CDS 25.0%
!! ATTGATTGAGTATTTTCAGA+AGG - chr8_4:52795246-52795265 Msa1332840:five_prime_UTR 25.0%
! CATAAACTTAGAGATAGTTA+TGG - chr8_4:52795282-52795301 Msa1332840:intron 25.0%
!! TTATAAGGTGTTAAATTTGC+AGG - chr8_4:52795401-52795420 Msa1332840:intron 25.0%
! GATTGTTCTTTATGAAATTG+AGG + chr8_4:52795636-52795655 None:intergenic 25.0%
! AGATTTACTGACAATAAAGA+TGG - chr8_4:52795765-52795784 Msa1332840:five_prime_UTR 25.0%
!!! TGACAATAAAGATGGTTTTA+CGG - chr8_4:52795773-52795792 Msa1332840:five_prime_UTR 25.0%
! TACATTGGAAGACAAAAAAA+GGG + chr8_4:52795820-52795839 None:intergenic 25.0%
! ATACATTGGAAGACAAAAAA+AGG + chr8_4:52795821-52795840 None:intergenic 25.0%
! CACAATGTAAAGAAATACAT+TGG + chr8_4:52795835-52795854 None:intergenic 25.0%
! AATAATTAGGTGATTACAGA+TGG + chr8_4:52795940-52795959 None:intergenic 25.0%
TGAATGAAAGAAAGAAAGAG+GGG + chr8_4:52793755-52793774 None:intergenic 30.0%
!! TTCTGTTAACTTGTAAGAAC+TGG + chr8_4:52794015-52794034 None:intergenic 30.0%
AGTTCTTACAAGTTAACAGA+AGG - chr8_4:52794014-52794033 Msa1332840:CDS 30.0%
! ATGGTTTTCTACAAATTGAG+GGG + chr8_4:52794137-52794156 None:intergenic 30.0%
! TGTAGTTTAGGTTAACAATC+AGG - chr8_4:52794237-52794256 Msa1332840:intron 30.0%
! GTAGTTTAGGTTAACAATCA+GGG - chr8_4:52794238-52794257 Msa1332840:intron 30.0%
TGATAAAAATATGAAAGGGG+AGG - chr8_4:52794372-52794391 Msa1332840:intron 30.0%
!! GTGAAGTTGATATTGAAAGT+GGG - chr8_4:52794398-52794417 Msa1332840:intron 30.0%
TGCTGATCAAAAGCTAATTA+GGG - chr8_4:52794723-52794742 Msa1332840:CDS 30.0%
TTCTGTATTGTCACATGATA+TGG - chr8_4:52794954-52794973 Msa1332840:CDS 30.0%
TCTGTATTGTCACATGATAT+GGG - chr8_4:52794955-52794974 Msa1332840:CDS 30.0%
!! GGTATTGTTTTGTTACCTAT+TGG - chr8_4:52795053-52795072 Msa1332840:CDS 30.0%
AAAACAGAACCAATAGGAAT+AGG + chr8_4:52795089-52795108 None:intergenic 30.0%
! ACTTTGAAAACAGAACCAAT+AGG + chr8_4:52795095-52795114 None:intergenic 30.0%
!!! TTGGTTCTGTTTTCAAAGTT+TGG - chr8_4:52795096-52795115 Msa1332840:CDS 30.0%
AACAATCCTGAAATTTGGAA+TGG + chr8_4:52795122-52795141 None:intergenic 30.0%
GAAGTAACAATCCTGAAATT+TGG + chr8_4:52795127-52795146 None:intergenic 30.0%
GGCAAAAATTGTATGATTGA+TGG + chr8_4:52795318-52795337 None:intergenic 30.0%
! CAATTTTGCGTACTTTTACT+GGG + chr8_4:52795339-52795358 None:intergenic 30.0%
CCTTATAAATGAATACCACT+AGG + chr8_4:52795389-52795408 None:intergenic 30.0%
CCTAGTGGTATTCATTTATA+AGG - chr8_4:52795386-52795405 Msa1332840:intron 30.0%
GAAAATATGCTTGCAATGAA+AGG + chr8_4:52795601-52795620 None:intergenic 30.0%
ACATTGGAAGACAAAAAAAG+GGG + chr8_4:52795819-52795838 None:intergenic 30.0%
! TACATTGTGATTTAAGGTGT+TGG - chr8_4:52795846-52795865 Msa1332840:five_prime_UTR 30.0%
! ACATTGTGATTTAAGGTGTT+GGG - chr8_4:52795847-52795866 Msa1332840:five_prime_UTR 30.0%
! GTTTCTTCTCTTTTCGTCAA+AGG - chr8_4:52793705-52793724 Msa1332840:three_prime_UTR 35.0%
AACTTGTAAGAACTGGTTAC+TGG + chr8_4:52794008-52794027 None:intergenic 35.0%
CTCCAATTATGAATGACGTT+GGG + chr8_4:52794049-52794068 None:intergenic 35.0%
ACTCCAATTATGAATGACGT+TGG + chr8_4:52794050-52794069 None:intergenic 35.0%
! TGGTTTTCTACAAATTGAGG+GGG + chr8_4:52794136-52794155 None:intergenic 35.0%
!!! TCTGTCTTTTGTTTTGATGC+AGG - chr8_4:52794190-52794209 Msa1332840:intron 35.0%
!!! CTGTCTTTTGTTTTGATGCA+GGG - chr8_4:52794191-52794210 Msa1332840:intron 35.0%
AAAAATTGCCTCTTTGTGAG+AGG - chr8_4:52794268-52794287 Msa1332840:intron 35.0%
TAAATGTTCCTCTCACAAAG+AGG + chr8_4:52794279-52794298 None:intergenic 35.0%
!!! TTAGTTTTGTGAGTTTGGGA+TGG - chr8_4:52794348-52794367 Msa1332840:intron 35.0%
AAAAATATGAAAGGGGAGGA+TGG - chr8_4:52794376-52794395 Msa1332840:intron 35.0%
!! GGTGAAGTTGATATTGAAAG+TGG - chr8_4:52794397-52794416 Msa1332840:intron 35.0%
! AGTTGATATTGAAAGTGGGT+TGG - chr8_4:52794402-52794421 Msa1332840:intron 35.0%
GGCAGTAACTAAAGATGATT+CGG - chr8_4:52794423-52794442 Msa1332840:intron 35.0%
GCAGTAACTAAAGATGATTC+GGG - chr8_4:52794424-52794443 Msa1332840:intron 35.0%
CGGGTAATACTAAACAAGAA+AGG - chr8_4:52794443-52794462 Msa1332840:intron 35.0%
AGAGTCTAACAAGTTGTTGA+CGG - chr8_4:52794585-52794604 Msa1332840:intron 35.0%
! GAAAAGTGTAGAGAATAGTC+CGG - chr8_4:52794621-52794640 Msa1332840:CDS 35.0%
! TTTTTCCGTTTCTCCTTAAC+CGG + chr8_4:52794643-52794662 None:intergenic 35.0%
AAGGAGAAACGGAAAAAATC+TGG - chr8_4:52794646-52794665 Msa1332840:CDS 35.0%
CTGCTGATCAAAAGCTAATT+AGG - chr8_4:52794722-52794741 Msa1332840:CDS 35.0%
TTGTCACATGATATGGGTTT+AGG - chr8_4:52794961-52794980 Msa1332840:CDS 35.0%
! TGTTACTTCTGTCCTTTGTT+TGG - chr8_4:52795138-52795157 Msa1332840:five_prime_UTR 35.0%
TTAATGGAACCACCAAACAA+AGG + chr8_4:52795153-52795172 None:intergenic 35.0%
! CCAATTTTGCGTACTTTTAC+TGG + chr8_4:52795340-52795359 None:intergenic 35.0%
! CCAGTAAAAGTACGCAAAAT+TGG - chr8_4:52795337-52795356 Msa1332840:intron 35.0%
AACAATCTCGATGTGATAAC+CGG - chr8_4:52795649-52795668 Msa1332840:intron 35.0%
AGAAAACTGAGATGAGATGA+AGG - chr8_4:52795735-52795754 Msa1332840:intron 35.0%
GAGAGAGATTTGAATGCAAC+AGG - chr8_4:52793600-52793619 Msa1332840:three_prime_UTR 40.0%
CGTCTATTCGCAAATTTCAG+GGG + chr8_4:52793922-52793941 None:intergenic 40.0%
GCGTCTATTCGCAAATTTCA+GGG + chr8_4:52793923-52793942 None:intergenic 40.0%
TCCTTCTGTTAACTTAGGCA+GGG - chr8_4:52793971-52793990 Msa1332840:CDS 40.0%
TGCCCAACGTCATTCATAAT+TGG - chr8_4:52794044-52794063 Msa1332840:CDS 40.0%
TCTACAATTTCAGGGGGAAT+TGG - chr8_4:52794078-52794097 Msa1332840:intron 40.0%
ACAAATTGAGGGGGTAACTA+GGG + chr8_4:52794127-52794146 None:intergenic 40.0%
TACAAATTGAGGGGGTAACT+AGG + chr8_4:52794128-52794147 None:intergenic 40.0%
TTAGGTTAACAATCAGGGTG+AGG - chr8_4:52794243-52794262 Msa1332840:intron 40.0%
GAGTCTAACAAGTTGTTGAC+GGG - chr8_4:52794586-52794605 Msa1332840:intron 40.0%
AGTCTAACAAGTTGTTGACG+GGG - chr8_4:52794587-52794606 Msa1332840:intron 40.0%
TGTAGAGAATAGTCCGGTTA+AGG - chr8_4:52794627-52794646 Msa1332840:CDS 40.0%
ATAGTCCGGTTAAGGAGAAA+CGG - chr8_4:52794635-52794654 Msa1332840:CDS 40.0%
ACTGTTGCAAGTTGTTGAGT+TGG - chr8_4:52795031-52795050 Msa1332840:CDS 40.0%
CTGTTGCAAGTTGTTGAGTT+GGG - chr8_4:52795032-52795051 Msa1332840:CDS 40.0%
ATAGGTGGAAATGGACCAAT+AGG + chr8_4:52795071-52795090 None:intergenic 40.0%
CCAATAGGAATAGGTGGAAA+TGG + chr8_4:52795080-52795099 None:intergenic 40.0%
CCATTTCCACCTATTCCTAT+TGG - chr8_4:52795077-52795096 Msa1332840:CDS 40.0%
ACAGAACCAATAGGAATAGG+TGG + chr8_4:52795086-52795105 None:intergenic 40.0%
GTTTGGCCATTCCAAATTTC+AGG - chr8_4:52795113-52795132 Msa1332840:exon 40.0%
! TACTTCTGTCCTTTGTTTGG+TGG - chr8_4:52795141-52795160 Msa1332840:five_prime_UTR 40.0%
TAGAACTCACTCACATGTCA+TGG - chr8_4:52795191-52795210 Msa1332840:five_prime_UTR 40.0%
TGAGAATAATGCACTCCTAG+TGG - chr8_4:52795371-52795390 Msa1332840:intron 40.0%
AGTTCAGTGACAAGTTTCCA+GGG - chr8_4:52795443-52795462 Msa1332840:intron 40.0%
! TACTTGTGAGACTCTGAAGA+AGG + chr8_4:52795560-52795579 None:intergenic 40.0%
CACAAGTAAGTAGAGATGCA+AGG - chr8_4:52795572-52795591 Msa1332840:intron 40.0%
CTACCTTACAGAATTGTACG+TGG - chr8_4:52795699-52795718 Msa1332840:intron 40.0%
GAACCACGTACAATTCTGTA+AGG + chr8_4:52795705-52795724 None:intergenic 40.0%
GGTAATTTATCCCCTGATGA+GGG - chr8_4:52795868-52795887 Msa1332840:five_prime_UTR 40.0%
TCTAAACTTCACCCTCATCA+GGG + chr8_4:52795882-52795901 None:intergenic 40.0%
AGGGTGAAGTTTAGACTTGT+AGG - chr8_4:52795887-52795906 Msa1332840:five_prime_UTR 40.0%
! GGGACAAAGAGCATAAGAGA+CGG + chr8_4:52793670-52793689 None:intergenic 45.0%
TATGCTCTTTGTCCCCTCAT+CGG - chr8_4:52793675-52793694 Msa1332840:three_prime_UTR 45.0%
AGAAACCCAAGATCCGATGA+GGG + chr8_4:52793691-52793710 None:intergenic 45.0%
AAGAAACCCAAGATCCGATG+AGG + chr8_4:52793692-52793711 None:intergenic 45.0%
!! TTTAGCTCGCCAATTTGCTG+TGG - chr8_4:52793883-52793902 Msa1332840:intron 45.0%
AATGTCCGACCACAGCAAAT+TGG + chr8_4:52793895-52793914 None:intergenic 45.0%
GGCGTCTATTCGCAAATTTC+AGG + chr8_4:52793924-52793943 None:intergenic 45.0%
CTCGGTCCTTCTGTTAACTT+AGG - chr8_4:52793966-52793985 Msa1332840:CDS 45.0%
GTCCTTCTGTTAACTTAGGC+AGG - chr8_4:52793970-52793989 Msa1332840:CDS 45.0%
GAGTGGAGATTCTGTGAATG+TGG - chr8_4:52794558-52794577 Msa1332840:intron 45.0%
GAAGACAACAGTGCAAACCA+TGG + chr8_4:52794857-52794876 None:intergenic 45.0%
TTTGCAGGCATGTCATCATC+TGG - chr8_4:52795416-52795435 Msa1332840:intron 45.0%
GAGTTCAGTGACAAGTTTCC+AGG - chr8_4:52795442-52795461 Msa1332840:intron 45.0%
GTTCAGTGACAAGTTTCCAG+GGG - chr8_4:52795444-52795463 Msa1332840:intron 45.0%
GGGTAATTTATCCCCTGATG+AGG - chr8_4:52795867-52795886 Msa1332840:five_prime_UTR 45.0%
CTAAACTTCACCCTCATCAG+GGG + chr8_4:52795881-52795900 None:intergenic 45.0%
GTCTAAACTTCACCCTCATC+AGG + chr8_4:52795883-52795902 None:intergenic 45.0%
AGAAGAGAACCACCGACAGT+TGG + chr8_4:52793496-52793515 None:intergenic 50.0%
GGAGAGAGAAGATGCTAGGA+AGG + chr8_4:52793521-52793540 None:intergenic 50.0%
AGCTGGAGAGAGAAGATGCT+AGG + chr8_4:52793525-52793544 None:intergenic 50.0%
TTTGTCCCCTCATCGGATCT+TGG - chr8_4:52793682-52793701 Msa1332840:three_prime_UTR 50.0%
TTGTCCCCTCATCGGATCTT+GGG - chr8_4:52793683-52793702 Msa1332840:three_prime_UTR 50.0%
GAAACCCAAGATCCGATGAG+GGG + chr8_4:52793690-52793709 None:intergenic 50.0%
GCCCTGCCTAAGTTAACAGA+AGG + chr8_4:52793975-52793994 None:intergenic 50.0%
TAACAAGTTGTTGACGGGGC+AGG - chr8_4:52794591-52794610 Msa1332840:CDS 50.0%
TGATCAGCAGCATCCAACGA+CGG + chr8_4:52794712-52794731 None:intergenic 50.0%
ATGGCAAACATGCTGCTGCT+TGG + chr8_4:52794838-52794857 None:intergenic 50.0%
AAGCAGCAGCATGTTTGCCA+TGG - chr8_4:52794837-52794856 Msa1332840:CDS 50.0%
GCAGCAGTGTTGCCAACTGT+CGG - chr8_4:52793481-52793500 Msa1332840:three_prime_UTR 55.0%
AGAGACGGACACTCACTCAC+AGG + chr8_4:52793655-52793674 None:intergenic 55.0%
!! GCTCGCCAATTTGCTGTGGT+CGG - chr8_4:52793887-52793906 Msa1332840:intron 55.0%
AAACCTCCTAGAGCTCCACG+AGG - chr8_4:52794685-52794704 Msa1332840:CDS 55.0%
AACCTCCTAGAGCTCCACGA+GGG - chr8_4:52794686-52794705 Msa1332840:CDS 55.0%
TTGCCATGTTGAAGCGTGCG+AGG - chr8_4:52794758-52794777 Msa1332840:CDS 55.0%
TGCCATGTTGAAGCGTGCGA+GGG - chr8_4:52794759-52794778 Msa1332840:CDS 55.0%
TCAGCTCCTCCTGCAGATAC+AGG + chr8_4:52795473-52795492 None:intergenic 55.0%
GGGCTTGTGTGAGATGCACA+AGG + chr8_4:52795799-52795818 None:intergenic 55.0%
GCAGTGTTGCCAACTGTCGG+TGG - chr8_4:52793484-52793503 Msa1332840:three_prime_UTR 60.0%
GTTGTTGACGGGGCAGGACT+TGG - chr8_4:52794597-52794616 Msa1332840:CDS 60.0%
CACCCTCGCACGCTTCAACA+TGG + chr8_4:52794764-52794783 None:intergenic 60.0%
CATGTTGAAGCGTGCGAGGG+TGG - chr8_4:52794762-52794781 Msa1332840:CDS 60.0%
CTGCAGATACAGGAGACCCC+TGG + chr8_4:52795463-52795482 None:intergenic 60.0%
CAGGGGTCTCCTGTATCTGC+AGG - chr8_4:52795461-52795480 Msa1332840:intron 60.0%
GGGTCTCCTGTATCTGCAGG+AGG - chr8_4:52795464-52795483 Msa1332840:intron 60.0%
!! GGCCCTCGTGGAGCTCTAGG+AGG + chr8_4:52794691-52794710 None:intergenic 70.0%
GACGGCCCTCGTGGAGCTCT+AGG + chr8_4:52794694-52794713 None:intergenic 70.0%
AGCTCCACGAGGGCCGTCGT+TGG - chr8_4:52794696-52794715 Msa1332840:CDS 70.0%
GCATCCAACGACGGCCCTCG+TGG + chr8_4:52794703-52794722 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 52793443 52796045 52793443 ID=Msa1332840;Name=Msa1332840
chr8_4 mRNA 52793443 52796045 52793443 ID=Msa1332840-mRNA-1;Parent=Msa1332840;Name=Msa1332840-mRNA-1;_AED=0.01;_eAED=0.01;_QI=439|1|1|1|0.66|0.5|4|296|241
chr8_4 exon 52793443 52793778 52793443 ID=Msa1332840-mRNA-1:exon:15995;Parent=Msa1332840-mRNA-1
chr8_4 exon 52793912 52794065 52793912 ID=Msa1332840-mRNA-1:exon:15994;Parent=Msa1332840-mRNA-1
chr8_4 exon 52794590 52795276 52794590 ID=Msa1332840-mRNA-1:exon:15993;Parent=Msa1332840-mRNA-1
chr8_4 exon 52795762 52796045 52795762 ID=Msa1332840-mRNA-1:exon:15992;Parent=Msa1332840-mRNA-1
chr8_4 five_prime_UTR 52795762 52796045 52795762 ID=Msa1332840-mRNA-1:five_prime_utr;Parent=Msa1332840-mRNA-1
chr8_4 five_prime_UTR 52795122 52795276 52795122 ID=Msa1332840-mRNA-1:five_prime_utr;Parent=Msa1332840-mRNA-1
chr8_4 CDS 52794590 52795121 52794590 ID=Msa1332840-mRNA-1:cds;Parent=Msa1332840-mRNA-1
chr8_4 CDS 52793912 52794065 52793912 ID=Msa1332840-mRNA-1:cds;Parent=Msa1332840-mRNA-1
chr8_4 CDS 52793739 52793778 52793739 ID=Msa1332840-mRNA-1:cds;Parent=Msa1332840-mRNA-1
chr8_4 three_prime_UTR 52793443 52793738 52793443 ID=Msa1332840-mRNA-1:three_prime_utr;Parent=Msa1332840-mRNA-1
Gene Sequence

>Msa1332840

ATGGATGATAAAAATATGAAAGGGGAGGATGGTGAAGTTGATATTGAAAGTGGGTTGGCAGTAACTAAAGATGATTCGGGTAATACTAAACAAGAAAGGACATTATTTGCTAAGATTTATTGTGGTATTGTTGGAGATTCTATAAAAGTTGAAGAAAGAAATAGTATATTATACTTGAAAGATTCAAATTTGAGTGGAGATTCTGTGAATGTGGCGAAAGAGTCTAACAAGTTGTTGACGGGGCAGGACTTGGTGAAAAGTGTAGAGAATAGTCCGGTTAAGGAGAAACGGAAAAAATCTGGTAACAAAAAGGCTGCTAAACCTCCTAGAGCTCCACGAGGGCCGTCGTTGGATGCTGCTGATCAAAAGCTAATTAGGGAGATTACTCAACTTGCCATGTTGAAGCGTGCGAGGGTGGAGCGTATGAAAGCTTTGAAAAAGATGAAAGCTGCTAAAGCATCTTCGTCTCCAAGCAGCAGCATGTTTGCCATGGTTTGCACTGTTGTCTTCTGTATTGTGATCTTATTTCAAGGCATGTCATCATCTGGAAAGAGTTCAGTGACAAGTTTCCAGGGGTCTCCTGTATCTGCAGGAGGAGCTGAAAGTGATCTGATTGCAGTTCAACTTCAACACCAATTGAATAAAATTTCAAGTCAACAACATGCACCTTCTTCAGAGTCTCACAAAATTGTACGTGGTTCATATTTGTCGAGAAAACTGAGATGA
Protein sequence

>Msa1332840

MDDKNMKGEDGEVDIESGLAVTKDDSGNTKQERTLFAKIYCGIVGDSIKVEERNSILYLKDSNLSGDSVNVAKESNKLLTGQDLVKSVENSPVKEKRKKSGNKKAAKPPRAPRGPSLDAADQKLIREITQLAMLKRARVERMKALKKMKAAKASSSPSSSMFAMVCTVVFCIVILFQGMSSSGKSSVTSFQGSPVSAGGAESDLIAVQLQHQLNKISSQQHAPSSESHKIVRGSYLSRKLR*