AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0343770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0343770 MtrunA17_Chr4g0068671 95.021 241 12 0 1 241 1 241 2.40e-139 390
Msa0343770 MtrunA17_Chr2g0294131 55.285 246 81 7 1 241 1 222 1.63e-53 172
Msa0343770 MtrunA17_Chr1g0194401 40.120 167 95 4 67 232 25 187 2.78e-28 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0343770 AT1G02380.1 34.649 228 124 10 3 225 2 209 5.59e-28 107
Msa0343770 AT3G17120.4 43.478 161 57 6 71 208 30 179 2.05e-26 102
Msa0343770 AT3G17120.2 43.478 161 57 6 71 208 30 179 2.05e-26 102
Msa0343770 AT3G17120.1 43.478 161 57 6 71 208 30 179 2.05e-26 102
Msa0343770 AT3G17120.5 43.478 161 57 6 71 208 30 179 2.05e-26 102
Msa0343770 AT3G17120.3 43.478 161 57 6 71 208 30 179 2.05e-26 102
Msa0343770 AT4G01960.1 50.495 101 47 3 113 213 106 203 2.12e-23 95.1
Msa0343770 AT4G01960.2 50.495 101 47 3 113 213 106 203 2.12e-23 95.1
Msa0343770 AT4G01960.3 65.152 66 22 1 113 178 106 170 2.40e-20 85.9

Find 82 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTTTACATTGTGATTTA+AGG 0.098041 3_1:+2228827 Msa0343770:three_prime_UTR
CTGCTGATCAAAAGCTAATT+AGG 0.140099 3_1:+2227709 Msa0343770:CDS
TTAACCTAAACTACAAAATT+AGG 0.172537 3_1:-2227216 None:intergenic
TTTGTCCCCTCATCGGATCT+TGG 0.258976 3_1:+2226670 Msa0343770:five_prime_UTR
TGTAGTTTAGGTTAACAATC+AGG 0.318334 3_1:+2227224 Msa0343770:five_prime_UTR
GACATTGATATAAAATAATT+AGG 0.333160 3_1:-2228937 None:intergenic
GAGTTCAGTGACAAGTTTCC+AGG 0.338873 3_1:+2228429 Msa0343770:CDS
GACATTGATATAAAATAATT+AGG 0.339980 3_1:-2228976 None:intergenic
TTGTCCCCTCATCGGATCTT+GGG 0.368020 3_1:+2226671 Msa0343770:five_prime_UTR
GGCAGTAACTAAAGATGATT+CGG 0.374127 3_1:+2227410 Msa0343770:CDS
GTCTAAACTTCACCCTCATC+AGG 0.389569 3_1:-2228867 None:intergenic
TGTATTGTGATCTTATTTCA+AGG 0.410724 3_1:+2227864 Msa0343770:CDS
AGGGTGAAGTTTAGACTTGT+AGG 0.415952 3_1:+2228874 Msa0343770:three_prime_UTR
TGTAGAGAATAGTCCGGTTA+AGG 0.419669 3_1:+2227614 Msa0343770:CDS
TGCTGATCAAAAGCTAATTA+GGG 0.424444 3_1:+2227710 Msa0343770:CDS
CAAAACTAATAATTAAACTT+TGG 0.429207 3_1:-2227321 None:intergenic
AAGATTTATTGTGGTATTGT+TGG 0.429970 3_1:+2227465 Msa0343770:CDS
TACATTGTGATTTAAGGTGT+TGG 0.433731 3_1:+2228833 Msa0343770:three_prime_UTR
CAGAGAGATTTGAATGCAAC+AGG 0.435389 3_1:+2226585 Msa0343770:five_prime_UTR
TTTGCAGGCATGTCATCATC+TGG 0.437634 3_1:+2228403 Msa0343770:intron
ACATTGTGATTTAAGGTGTT+GGG 0.440893 3_1:+2228834 Msa0343770:three_prime_UTR
TCAGCTCCTCCTGCAGATAC+AGG 0.451996 3_1:-2228457 None:intergenic
CTAAACTACAAAATTAGGTT+AGG 0.452713 3_1:-2227211 None:intergenic
CAGGGGTCTCCTGTATCTGC+AGG 0.453931 3_1:+2228448 Msa0343770:CDS
GCAGTAACTAAAGATGATTC+GGG 0.461926 3_1:+2227411 Msa0343770:CDS
GGTGAAGTTGATATTGAAAG+TGG 0.477042 3_1:+2227384 Msa0343770:CDS
ATGGCAAACATGCTGCTGCT+TGG 0.493125 3_1:-2227822 None:intergenic
AGATTTACTGACAATAAAGA+TGG 0.495380 3_1:+2228752 Msa0343770:three_prime_UTR
TATGCTCTTTGTCCCCTCAT+CGG 0.500303 3_1:+2226663 Msa0343770:five_prime_UTR
GTTGTTGACGGGGCAGGACT+TGG 0.513107 3_1:+2227584 Msa0343770:CDS
GAGTCTAACAAGTTGTTGAC+GGG 0.513280 3_1:+2227573 Msa0343770:CDS
AGCTCCACGAGGGCCGTCGT+TGG 0.517134 3_1:+2227683 Msa0343770:CDS
GTAGTTTAGGTTAACAATCA+GGG 0.520089 3_1:+2227225 Msa0343770:five_prime_UTR
CACCCTCGCACGCTTCAACA+TGG 0.521089 3_1:-2227748 None:intergenic
ATAGTCCGGTTAAGGAGAAA+CGG 0.534537 3_1:+2227622 Msa0343770:CDS
TTGAAAGATTCAAATTTGAG+TGG 0.539571 3_1:+2227528 Msa0343770:CDS
AAGCAGCAGCATGTTTGCCA+TGG 0.544835 3_1:+2227824 Msa0343770:CDS
TAACAAGTTGTTGACGGGGC+AGG 0.545014 3_1:+2227578 Msa0343770:CDS
AATAATTAGGTGATTACAGA+TGG 0.545580 3_1:-2228924 None:intergenic
TTATTTGCTAAGATTTATTG+TGG 0.547930 3_1:+2227456 Msa0343770:CDS
CGGGTAATACTAAACAAGAA+AGG 0.549432 3_1:+2227430 Msa0343770:CDS
GACGGCCCTCGTGGAGCTCT+AGG 0.551220 3_1:-2227678 None:intergenic
GGAGAGAGAAGATGCTAGGA+AGG 0.551589 3_1:-2226501 None:intergenic
AGAAAACTGAGATGAGATGA+AGG 0.556919 3_1:+2228722 Msa0343770:exon
TAAATGTTCCTCTCACAAAG+AGG 0.559209 3_1:-2227263 None:intergenic
GGGTAATTTATCCCCTGATG+AGG 0.559462 3_1:+2228854 Msa0343770:three_prime_UTR
CACAATGTAAAGAAATACAT+TGG 0.560595 3_1:-2228819 None:intergenic
CATGTTGAAGCGTGCGAGGG+TGG 0.561704 3_1:+2227749 Msa0343770:CDS
TTAGGTTAACAATCAGGGTG+AGG 0.567911 3_1:+2227230 Msa0343770:five_prime_UTR
AGAGTCTAACAAGTTGTTGA+CGG 0.570293 3_1:+2227572 Msa0343770:CDS
GAAAAGTGTAGAGAATAGTC+CGG 0.572405 3_1:+2227608 Msa0343770:CDS
GTGAAGTTGATATTGAAAGT+GGG 0.572872 3_1:+2227385 Msa0343770:CDS
GGTAATTTATCCCCTGATGA+GGG 0.577082 3_1:+2228855 Msa0343770:three_prime_UTR
TCTAAACTTCACCCTCATCA+GGG 0.581373 3_1:-2228866 None:intergenic
AGAAGAGAACCACCGACAGT+TGG 0.582996 3_1:-2226476 None:intergenic
AGTTCAGTGACAAGTTTCCA+GGG 0.583334 3_1:+2228430 Msa0343770:CDS
GCATCCAACGACGGCCCTCG+TGG 0.589028 3_1:-2227687 None:intergenic
CTGCAGATACAGGAGACCCC+TGG 0.589034 3_1:-2228447 None:intergenic
TACTTGTGAGACTCTGAAGA+AGG 0.591671 3_1:-2228544 None:intergenic
GGGTCTCCTGTATCTGCAGG+AGG 0.593983 3_1:+2228451 Msa0343770:CDS
AGCTGGAGAGAGAAGATGCT+AGG 0.600356 3_1:-2226505 None:intergenic
TTGCCATGTTGAAGCGTGCG+AGG 0.607905 3_1:+2227745 Msa0343770:CDS
AGAGACGGACACTCACTCAC+AGG 0.610518 3_1:-2226640 None:intergenic
AGAAACCCAAGATCCGATGA+GGG 0.614811 3_1:-2226676 None:intergenic
AGTTGATATTGAAAGTGGGT+TGG 0.627313 3_1:+2227389 Msa0343770:CDS
AAGAAACCCAAGATCCGATG+AGG 0.644513 3_1:-2226677 None:intergenic
CTACCTTACAGAATTGTACG+TGG 0.646628 3_1:+2228686 Msa0343770:intron
GAGTGGAGATTCTGTGAATG+TGG 0.653592 3_1:+2227545 Msa0343770:CDS
TGATCAGCAGCATCCAACGA+CGG 0.653843 3_1:-2227696 None:intergenic
TGCCATGTTGAAGCGTGCGA+GGG 0.655794 3_1:+2227746 Msa0343770:CDS
CTAAACTTCACCCTCATCAG+GGG 0.659411 3_1:-2228865 None:intergenic
GGCCCTCGTGGAGCTCTAGG+AGG 0.662072 3_1:-2227675 None:intergenic
GGGCTTGTGTGAGATGCACA+AGG 0.666533 3_1:-2228783 None:intergenic
GCAGCAGTGTTGCCAACTGT+CGG 0.673083 3_1:+2226464 Msa0343770:five_prime_UTR
GAAACCCAAGATCCGATGAG+GGG 0.673655 3_1:-2226675 None:intergenic
GGGACAAAGAGCATAAGAGA+CGG 0.674570 3_1:-2226655 None:intergenic
GTTCAGTGACAAGTTTCCAG+GGG 0.676920 3_1:+2228431 Msa0343770:CDS
GAAGACAACAGTGCAAACCA+TGG 0.690412 3_1:-2227841 None:intergenic
AGTCTAACAAGTTGTTGACG+GGG 0.706378 3_1:+2227574 Msa0343770:CDS
GCAGTGTTGCCAACTGTCGG+TGG 0.713536 3_1:+2226467 Msa0343770:five_prime_UTR
AACCTCCTAGAGCTCCACGA+GGG 0.737863 3_1:+2227673 Msa0343770:CDS
AAACCTCCTAGAGCTCCACG+AGG 0.781040 3_1:+2227672 Msa0343770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAACTAATAATTAAACTT+TGG - chr3_1:2227324-2227343 None:intergenic 15.0%
!! GACATTGATATAAAATAATT+AGG - chr3_1:2228940-2228959 None:intergenic 15.0%
!! GACATTGATATAAAATAATT+AGG - chr3_1:2228940-2228959 None:intergenic 15.0%
!!! GTTTTATTGTAAATGAAAGA+AGG - chr3_1:2226721-2226740 None:intergenic 20.0%
!! AAAAAATGAATGACATTGAA+GGG - chr3_1:2226776-2226795 None:intergenic 20.0%
!! TATTGCTATGTTTAATTCAT+TGG + chr3_1:2226847-2226866 Msa0343770:intron 20.0%
!!! TGTCTGATATCATTTAAAAT+TGG - chr3_1:2226932-2226951 None:intergenic 20.0%
!!! TCTTTTTTTTCTACAATTTC+AGG + chr3_1:2227056-2227075 Msa0343770:intron 20.0%
!!! CTTTTTTTTCTACAATTTCA+GGG + chr3_1:2227057-2227076 Msa0343770:intron 20.0%
!!! TTTTTTTTCTACAATTTCAG+GGG + chr3_1:2227058-2227077 Msa0343770:intron 20.0%
!! AATTGCAAATGACAAAAAAA+TGG - chr3_1:2227143-2227162 None:intergenic 20.0%
!! TTAACCTAAACTACAAAATT+AGG - chr3_1:2227219-2227238 None:intergenic 20.0%
!!! AATTATTAGTTTTGTGAGTT+TGG + chr3_1:2227330-2227349 Msa0343770:five_prime_UTR 20.0%
!!! ATTATTAGTTTTGTGAGTTT+GGG + chr3_1:2227331-2227350 Msa0343770:five_prime_UTR 20.0%
!! ATGGATGATAAAAATATGAA+AGG + chr3_1:2227354-2227373 Msa0343770:CDS 20.0%
!! TGGATGATAAAAATATGAAA+GGG + chr3_1:2227355-2227374 Msa0343770:CDS 20.0%
!!! TTATTTGCTAAGATTTATTG+TGG + chr3_1:2227456-2227475 Msa0343770:CDS 20.0%
!! ACAATTGACAAATGATATAA+TGG + chr3_1:2227980-2227999 Msa0343770:intron 20.0%
!! TACTCAATCAATATGAAATT+CGG - chr3_1:2228225-2228244 None:intergenic 20.0%
!!! GACTTGAAATTTTATTCAAT+TGG - chr3_1:2228513-2228532 None:intergenic 20.0%
!! AACAATAAGAATATTACTAC+CGG - chr3_1:2228658-2228677 None:intergenic 20.0%
!! TTTCTTTACATTGTGATTTA+AGG + chr3_1:2228827-2228846 Msa0343770:three_prime_UTR 20.0%
! AAAAATGGGATTTATATAGC+TGG - chr3_1:2226525-2226544 None:intergenic 25.0%
! TCTGTGATTCTTCAAAAAAT+GGG - chr3_1:2226539-2226558 None:intergenic 25.0%
! ATCTGTGATTCTTCAAAAAA+TGG - chr3_1:2226540-2226559 None:intergenic 25.0%
! ATGAATGAAAGAAAGAAAGA+GGG - chr3_1:2226744-2226763 None:intergenic 25.0%
! AATGAATGAAAGAAAGAAAG+AGG - chr3_1:2226745-2226764 None:intergenic 25.0%
! GAAAAAATGAATGACATTGA+AGG - chr3_1:2226777-2226796 None:intergenic 25.0%
! TTAAATGATATCAGACAACT+CGG + chr3_1:2226935-2226954 Msa0343770:intron 25.0%
!!! TTTTTTTCTACAATTTCAGG+GGG + chr3_1:2227059-2227078 Msa0343770:intron 25.0%
!! AATGGTTTTCTACAAATTGA+GGG - chr3_1:2227125-2227144 None:intergenic 25.0%
!! AAATGGTTTTCTACAAATTG+AGG - chr3_1:2227126-2227145 None:intergenic 25.0%
! CTAAACTACAAAATTAGGTT+AGG - chr3_1:2227214-2227233 None:intergenic 25.0%
!! CTAACCTAATTTTGTAGTTT+AGG + chr3_1:2227212-2227231 Msa0343770:five_prime_UTR 25.0%
! GGATGATAAAAATATGAAAG+GGG + chr3_1:2227356-2227375 Msa0343770:CDS 25.0%
! AAGATTTATTGTGGTATTGT+TGG + chr3_1:2227465-2227484 Msa0343770:CDS 25.0%
! TTGAAAGATTCAAATTTGAG+TGG + chr3_1:2227528-2227547 Msa0343770:CDS 25.0%
! GAAAAAATCTGGTAACAAAA+AGG + chr3_1:2227644-2227663 Msa0343770:CDS 25.0%
!! TGTATTGTGATCTTATTTCA+AGG + chr3_1:2227864-2227883 Msa0343770:CDS 25.0%
! AATTAAAGTAGTATCTGTCA+TGG + chr3_1:2227897-2227916 Msa0343770:intron 25.0%
!! ATTGATTGAGTATTTTCAGA+AGG + chr3_1:2228233-2228252 Msa0343770:intron 25.0%
! CATAAACTTAGAGATAGTTA+TGG + chr3_1:2228269-2228288 Msa0343770:intron 25.0%
!! TTATAAGGTGTTAAATTTGC+AGG + chr3_1:2228388-2228407 Msa0343770:intron 25.0%
! GATTGTTCTTTATGAAATTG+AGG - chr3_1:2228623-2228642 None:intergenic 25.0%
! AGATTTACTGACAATAAAGA+TGG + chr3_1:2228752-2228771 Msa0343770:three_prime_UTR 25.0%
!!! TGACAATAAAGATGGTTTTA+CGG + chr3_1:2228760-2228779 Msa0343770:three_prime_UTR 25.0%
! TACATTGGAAGACAAAAAAA+GGG - chr3_1:2228807-2228826 None:intergenic 25.0%
! ATACATTGGAAGACAAAAAA+AGG - chr3_1:2228808-2228827 None:intergenic 25.0%
! CACAATGTAAAGAAATACAT+TGG - chr3_1:2228822-2228841 None:intergenic 25.0%
! AATAATTAGGTGATTACAGA+TGG - chr3_1:2228927-2228946 None:intergenic 25.0%
TGAATGAAAGAAAGAAAGAG+GGG - chr3_1:2226743-2226762 None:intergenic 30.0%
!! TTCTGTTAACTTGTAAGAAC+TGG - chr3_1:2227002-2227021 None:intergenic 30.0%
AGTTCTTACAAGTTAACAGA+AGG + chr3_1:2227001-2227020 Msa0343770:intron 30.0%
! ATGGTTTTCTACAAATTGAG+GGG - chr3_1:2227124-2227143 None:intergenic 30.0%
! TGTAGTTTAGGTTAACAATC+AGG + chr3_1:2227224-2227243 Msa0343770:five_prime_UTR 30.0%
! GTAGTTTAGGTTAACAATCA+GGG + chr3_1:2227225-2227244 Msa0343770:five_prime_UTR 30.0%
TGATAAAAATATGAAAGGGG+AGG + chr3_1:2227359-2227378 Msa0343770:CDS 30.0%
!! GTGAAGTTGATATTGAAAGT+GGG + chr3_1:2227385-2227404 Msa0343770:CDS 30.0%
TGCTGATCAAAAGCTAATTA+GGG + chr3_1:2227710-2227729 Msa0343770:CDS 30.0%
TCTGTATTGTCACATGATAT+AGG + chr3_1:2227942-2227961 Msa0343770:intron 30.0%
TTGTCACATGATATAGGTTT+AGG + chr3_1:2227948-2227967 Msa0343770:intron 30.0%
!! GGTATTGTTTTGTTACCTAT+TGG + chr3_1:2228040-2228059 Msa0343770:intron 30.0%
AAAACAGAACCAATAGGAAT+AGG - chr3_1:2228076-2228095 None:intergenic 30.0%
! ACTTTGAAAACAGAACCAAT+AGG - chr3_1:2228082-2228101 None:intergenic 30.0%
!!! TTGGTTCTGTTTTCAAAGTT+TGG + chr3_1:2228083-2228102 Msa0343770:intron 30.0%
AACAATCCTGAAATTTGGAA+TGG - chr3_1:2228109-2228128 None:intergenic 30.0%
GAAGTAACAATCCTGAAATT+TGG - chr3_1:2228114-2228133 None:intergenic 30.0%
GGCAAAAATTGTATGATTGA+TGG - chr3_1:2228305-2228324 None:intergenic 30.0%
! CAATTTTGCGTACTTTTACT+GGG - chr3_1:2228326-2228345 None:intergenic 30.0%
CCTTATAAATGAATACCACT+AGG - chr3_1:2228376-2228395 None:intergenic 30.0%
CCTAGTGGTATTCATTTATA+AGG + chr3_1:2228373-2228392 Msa0343770:intron 30.0%
GAAAATATGCTTGCAATGAA+AGG - chr3_1:2228588-2228607 None:intergenic 30.0%
ACATTGGAAGACAAAAAAAG+GGG - chr3_1:2228806-2228825 None:intergenic 30.0%
! TACATTGTGATTTAAGGTGT+TGG + chr3_1:2228833-2228852 Msa0343770:three_prime_UTR 30.0%
! ACATTGTGATTTAAGGTGTT+GGG + chr3_1:2228834-2228853 Msa0343770:three_prime_UTR 30.0%
! GTTTCTTCTCTTTTCGTCAA+AGG + chr3_1:2226693-2226712 Msa0343770:five_prime_UTR 35.0%
AACTTGTAAGAACTGGTTAC+TGG - chr3_1:2226995-2227014 None:intergenic 35.0%
CTCCAATTATGAATGACGTT+GGG - chr3_1:2227036-2227055 None:intergenic 35.0%
ACTCCAATTATGAATGACGT+TGG - chr3_1:2227037-2227056 None:intergenic 35.0%
! TGGTTTTCTACAAATTGAGG+GGG - chr3_1:2227123-2227142 None:intergenic 35.0%
!!! TCTGTCTTTTGTTTTGATGC+AGG + chr3_1:2227177-2227196 Msa0343770:intron 35.0%
!!! CTGTCTTTTGTTTTGATGCA+GGG + chr3_1:2227178-2227197 Msa0343770:intron 35.0%
AAAAATTGCCTCTTTGTGAG+AGG + chr3_1:2227255-2227274 Msa0343770:five_prime_UTR 35.0%
TAAATGTTCCTCTCACAAAG+AGG - chr3_1:2227266-2227285 None:intergenic 35.0%
!!! TTAGTTTTGTGAGTTTGGGA+TGG + chr3_1:2227335-2227354 Msa0343770:exon 35.0%
AAAAATATGAAAGGGGAGGA+TGG + chr3_1:2227363-2227382 Msa0343770:CDS 35.0%
!! GGTGAAGTTGATATTGAAAG+TGG + chr3_1:2227384-2227403 Msa0343770:CDS 35.0%
! AGTTGATATTGAAAGTGGGT+TGG + chr3_1:2227389-2227408 Msa0343770:CDS 35.0%
GGCAGTAACTAAAGATGATT+CGG + chr3_1:2227410-2227429 Msa0343770:CDS 35.0%
GCAGTAACTAAAGATGATTC+GGG + chr3_1:2227411-2227430 Msa0343770:CDS 35.0%
CGGGTAATACTAAACAAGAA+AGG + chr3_1:2227430-2227449 Msa0343770:CDS 35.0%
AGAGTCTAACAAGTTGTTGA+CGG + chr3_1:2227572-2227591 Msa0343770:CDS 35.0%
! GAAAAGTGTAGAGAATAGTC+CGG + chr3_1:2227608-2227627 Msa0343770:CDS 35.0%
! TTTTTCCGTTTCTCCTTAAC+CGG - chr3_1:2227630-2227649 None:intergenic 35.0%
AAGGAGAAACGGAAAAAATC+TGG + chr3_1:2227633-2227652 Msa0343770:CDS 35.0%
CTGCTGATCAAAAGCTAATT+AGG + chr3_1:2227709-2227728 Msa0343770:CDS 35.0%
! TGTTACTTCTGTCCTTTGTT+TGG + chr3_1:2228125-2228144 Msa0343770:intron 35.0%
! CCAATTTTGCGTACTTTTAC+TGG - chr3_1:2228327-2228346 None:intergenic 35.0%
! CCAGTAAAAGTACGCAAAAT+TGG + chr3_1:2228324-2228343 Msa0343770:intron 35.0%
AACAATCTCGATGTGATAAC+CGG + chr3_1:2228636-2228655 Msa0343770:intron 35.0%
AGAAAACTGAGATGAGATGA+AGG + chr3_1:2228722-2228741 Msa0343770:exon 35.0%
CAGAGAGATTTGAATGCAAC+AGG + chr3_1:2226585-2226604 Msa0343770:five_prime_UTR 40.0%
CGTCTATTCGCAAATTTCAG+GGG - chr3_1:2226909-2226928 None:intergenic 40.0%
GCGTCTATTCGCAAATTTCA+GGG - chr3_1:2226910-2226929 None:intergenic 40.0%
TGCCCAACGTCATTCATAAT+TGG + chr3_1:2227031-2227050 Msa0343770:intron 40.0%
TCTACAATTTCAGGGGGAAT+TGG + chr3_1:2227065-2227084 Msa0343770:intron 40.0%
ACAAATTGAGGGGGTAACTA+GGG - chr3_1:2227114-2227133 None:intergenic 40.0%
TACAAATTGAGGGGGTAACT+AGG - chr3_1:2227115-2227134 None:intergenic 40.0%
TTAGGTTAACAATCAGGGTG+AGG + chr3_1:2227230-2227249 Msa0343770:five_prime_UTR 40.0%
GAGTCTAACAAGTTGTTGAC+GGG + chr3_1:2227573-2227592 Msa0343770:CDS 40.0%
AGTCTAACAAGTTGTTGACG+GGG + chr3_1:2227574-2227593 Msa0343770:CDS 40.0%
TGTAGAGAATAGTCCGGTTA+AGG + chr3_1:2227614-2227633 Msa0343770:CDS 40.0%
ATAGTCCGGTTAAGGAGAAA+CGG + chr3_1:2227622-2227641 Msa0343770:CDS 40.0%
ACTGTTGCAAGTTGTTGAGT+TGG + chr3_1:2228018-2228037 Msa0343770:intron 40.0%
CTGTTGCAAGTTGTTGAGTT+GGG + chr3_1:2228019-2228038 Msa0343770:intron 40.0%
ATAGGTGGAAATGGACCAAT+AGG - chr3_1:2228058-2228077 None:intergenic 40.0%
CCAATAGGAATAGGTGGAAA+TGG - chr3_1:2228067-2228086 None:intergenic 40.0%
CCATTTCCACCTATTCCTAT+TGG + chr3_1:2228064-2228083 Msa0343770:intron 40.0%
ACAGAACCAATAGGAATAGG+TGG - chr3_1:2228073-2228092 None:intergenic 40.0%
GTTTGGCCATTCCAAATTTC+AGG + chr3_1:2228100-2228119 Msa0343770:intron 40.0%
! TACTTCTGTCCTTTGTTTGG+TGG + chr3_1:2228128-2228147 Msa0343770:intron 40.0%
TTGATGGAACCACCAAACAA+AGG - chr3_1:2228140-2228159 None:intergenic 40.0%
TAGAACTCACTCACATGTCA+TGG + chr3_1:2228178-2228197 Msa0343770:intron 40.0%
TGAGAATAATGCACTCCTAG+TGG + chr3_1:2228358-2228377 Msa0343770:intron 40.0%
AGTTCAGTGACAAGTTTCCA+GGG + chr3_1:2228430-2228449 Msa0343770:CDS 40.0%
! TACTTGTGAGACTCTGAAGA+AGG - chr3_1:2228547-2228566 None:intergenic 40.0%
CACAAGTAAGTAGAGATGCA+AGG + chr3_1:2228559-2228578 Msa0343770:intron 40.0%
CTACCTTACAGAATTGTACG+TGG + chr3_1:2228686-2228705 Msa0343770:intron 40.0%
GAACCACGTACAATTCTGTA+AGG - chr3_1:2228692-2228711 None:intergenic 40.0%
GGTAATTTATCCCCTGATGA+GGG + chr3_1:2228855-2228874 Msa0343770:three_prime_UTR 40.0%
TCTAAACTTCACCCTCATCA+GGG - chr3_1:2228869-2228888 None:intergenic 40.0%
AGGGTGAAGTTTAGACTTGT+AGG + chr3_1:2228874-2228893 Msa0343770:three_prime_UTR 40.0%
! GGGACAAAGAGCATAAGAGA+CGG - chr3_1:2226658-2226677 None:intergenic 45.0%
TATGCTCTTTGTCCCCTCAT+CGG + chr3_1:2226663-2226682 Msa0343770:five_prime_UTR 45.0%
AGAAACCCAAGATCCGATGA+GGG - chr3_1:2226679-2226698 None:intergenic 45.0%
AAGAAACCCAAGATCCGATG+AGG - chr3_1:2226680-2226699 None:intergenic 45.0%
!! TTTAGCTCGCCAATTTGCTG+TGG + chr3_1:2226870-2226889 Msa0343770:intron 45.0%
AATGTCCGACCACAGCAAAT+TGG - chr3_1:2226882-2226901 None:intergenic 45.0%
GGCGTCTATTCGCAAATTTC+AGG - chr3_1:2226911-2226930 None:intergenic 45.0%
ACTCGGTCCTTCTGTTAACT+TGG + chr3_1:2226952-2226971 Msa0343770:intron 45.0%
CTCGGTCCTTCTGTTAACTT+GGG + chr3_1:2226953-2226972 Msa0343770:intron 45.0%
! TCCTTCTGTTAACTTGGGCA+GGG + chr3_1:2226958-2226977 Msa0343770:intron 45.0%
GAGTGGAGATTCTGTGAATG+TGG + chr3_1:2227545-2227564 Msa0343770:CDS 45.0%
GAAGACAACAGTGCAAACCA+TGG - chr3_1:2227844-2227863 None:intergenic 45.0%
TTTGCAGGCATGTCATCATC+TGG + chr3_1:2228403-2228422 Msa0343770:intron 45.0%
GAGTTCAGTGACAAGTTTCC+AGG + chr3_1:2228429-2228448 Msa0343770:CDS 45.0%
GTTCAGTGACAAGTTTCCAG+GGG + chr3_1:2228431-2228450 Msa0343770:CDS 45.0%
GGGTAATTTATCCCCTGATG+AGG + chr3_1:2228854-2228873 Msa0343770:three_prime_UTR 45.0%
CTAAACTTCACCCTCATCAG+GGG - chr3_1:2228868-2228887 None:intergenic 45.0%
GTCTAAACTTCACCCTCATC+AGG - chr3_1:2228870-2228889 None:intergenic 45.0%
!!! AAAATAATAATAAATTTTGA+TGG - chr3_1:2228156-2228175 None:intergenic 5.0%
!!! ATCAAAATTTATTATTATTT+TGG + chr3_1:2228155-2228174 Msa0343770:intron 5.0%
AGAAGAGAACCACCGACAGT+TGG - chr3_1:2226479-2226498 None:intergenic 50.0%
GGAGAGAGAAGATGCTAGGA+AGG - chr3_1:2226504-2226523 None:intergenic 50.0%
AGCTGGAGAGAGAAGATGCT+AGG - chr3_1:2226508-2226527 None:intergenic 50.0%
TTTGTCCCCTCATCGGATCT+TGG + chr3_1:2226670-2226689 Msa0343770:five_prime_UTR 50.0%
TTGTCCCCTCATCGGATCTT+GGG + chr3_1:2226671-2226690 Msa0343770:five_prime_UTR 50.0%
GAAACCCAAGATCCGATGAG+GGG - chr3_1:2226678-2226697 None:intergenic 50.0%
! GTCCTTCTGTTAACTTGGGC+AGG + chr3_1:2226957-2226976 Msa0343770:intron 50.0%
TAACAAGTTGTTGACGGGGC+AGG + chr3_1:2227578-2227597 Msa0343770:CDS 50.0%
TGATCAGCAGCATCCAACGA+CGG - chr3_1:2227699-2227718 None:intergenic 50.0%
ATGGCAAACATGCTGCTGCT+TGG - chr3_1:2227825-2227844 None:intergenic 50.0%
AAGCAGCAGCATGTTTGCCA+TGG + chr3_1:2227824-2227843 Msa0343770:CDS 50.0%
GCAGCAGTGTTGCCAACTGT+CGG + chr3_1:2226464-2226483 Msa0343770:five_prime_UTR 55.0%
AGAGACGGACACTCACTCAC+AGG - chr3_1:2226643-2226662 None:intergenic 55.0%
!! GCTCGCCAATTTGCTGTGGT+CGG + chr3_1:2226874-2226893 Msa0343770:intron 55.0%
GCCCTGCCCAAGTTAACAGA+AGG - chr3_1:2226962-2226981 None:intergenic 55.0%
AAACCTCCTAGAGCTCCACG+AGG + chr3_1:2227672-2227691 Msa0343770:CDS 55.0%
AACCTCCTAGAGCTCCACGA+GGG + chr3_1:2227673-2227692 Msa0343770:CDS 55.0%
TTGCCATGTTGAAGCGTGCG+AGG + chr3_1:2227745-2227764 Msa0343770:CDS 55.0%
TGCCATGTTGAAGCGTGCGA+GGG + chr3_1:2227746-2227765 Msa0343770:CDS 55.0%
TCAGCTCCTCCTGCAGATAC+AGG - chr3_1:2228460-2228479 None:intergenic 55.0%
GGGCTTGTGTGAGATGCACA+AGG - chr3_1:2228786-2228805 None:intergenic 55.0%
GCAGTGTTGCCAACTGTCGG+TGG + chr3_1:2226467-2226486 Msa0343770:five_prime_UTR 60.0%
GTTGTTGACGGGGCAGGACT+TGG + chr3_1:2227584-2227603 Msa0343770:CDS 60.0%
CACCCTCGCACGCTTCAACA+TGG - chr3_1:2227751-2227770 None:intergenic 60.0%
CATGTTGAAGCGTGCGAGGG+TGG + chr3_1:2227749-2227768 Msa0343770:CDS 60.0%
CTGCAGATACAGGAGACCCC+TGG - chr3_1:2228450-2228469 None:intergenic 60.0%
CAGGGGTCTCCTGTATCTGC+AGG + chr3_1:2228448-2228467 Msa0343770:CDS 60.0%
GGGTCTCCTGTATCTGCAGG+AGG + chr3_1:2228451-2228470 Msa0343770:CDS 60.0%
!! GGCCCTCGTGGAGCTCTAGG+AGG - chr3_1:2227678-2227697 None:intergenic 70.0%
GACGGCCCTCGTGGAGCTCT+AGG - chr3_1:2227681-2227700 None:intergenic 70.0%
AGCTCCACGAGGGCCGTCGT+TGG + chr3_1:2227683-2227702 Msa0343770:CDS 70.0%
GCATCCAACGACGGCCCTCG+TGG - chr3_1:2227690-2227709 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 2226426 2229032 2226426 ID=Msa0343770;Name=Msa0343770
chr3_1 mRNA 2226426 2229032 2226426 ID=Msa0343770-mRNA-1;Parent=Msa0343770;Name=Msa0343770-mRNA-1;_AED=0.01;_eAED=0.01;_QI=444|1|1|1|0.66|0.5|4|296|241
chr3_1 exon 2226426 2226714 2226426 ID=Msa0343770-mRNA-1:exon:673;Parent=Msa0343770-mRNA-1
chr3_1 exon 2227199 2227885 2227199 ID=Msa0343770-mRNA-1:exon:674;Parent=Msa0343770-mRNA-1
chr3_1 exon 2228410 2228563 2228410 ID=Msa0343770-mRNA-1:exon:675;Parent=Msa0343770-mRNA-1
chr3_1 exon 2228697 2229032 2228697 ID=Msa0343770-mRNA-1:exon:676;Parent=Msa0343770-mRNA-1
chr3_1 five_prime_UTR 2226426 2226714 2226426 ID=Msa0343770-mRNA-1:five_prime_utr;Parent=Msa0343770-mRNA-1
chr3_1 five_prime_UTR 2227199 2227353 2227199 ID=Msa0343770-mRNA-1:five_prime_utr;Parent=Msa0343770-mRNA-1
chr3_1 CDS 2227354 2227885 2227354 ID=Msa0343770-mRNA-1:cds;Parent=Msa0343770-mRNA-1
chr3_1 CDS 2228410 2228563 2228410 ID=Msa0343770-mRNA-1:cds;Parent=Msa0343770-mRNA-1
chr3_1 CDS 2228697 2228736 2228697 ID=Msa0343770-mRNA-1:cds;Parent=Msa0343770-mRNA-1
chr3_1 three_prime_UTR 2228737 2229032 2228737 ID=Msa0343770-mRNA-1:three_prime_utr;Parent=Msa0343770-mRNA-1
Gene Sequence

>Msa0343770

ATGGATGATAAAAATATGAAAGGGGAGGATGGTGAAGTTGATATTGAAAGTGGGTTGGCAGTAACTAAAGATGATTCGGGTAATACTAAACAAGAAAGGACATTATTTGCTAAGATTTATTGTGGTATTGTTGGAGATTCTATAAAAGTTGAAGAAAGAAATAGTATATTATACTTGAAAGATTCAAATTTGAGTGGAGATTCTGTGAATGTGGCGAAAGAGTCTAACAAGTTGTTGACGGGGCAGGACTTGGTGAAAAGTGTAGAGAATAGTCCGGTTAAGGAGAAACGGAAAAAATCTGGTAACAAAAAGGCTGCTAAACCTCCTAGAGCTCCACGAGGGCCGTCGTTGGATGCTGCTGATCAAAAGCTAATTAGGGAGATTACTCAACTTGCCATGTTGAAGCGTGCGAGGGTGGAGCGTATGAAAGCTTTGAAAAAGATGAAAGCTGCTAAAGCATCTTCGTCTCCAAGCAGCAGCATGTTTGCCATGGTTTGCACTGTTGTCTTCTGTATTGTGATCTTATTTCAAGGCATGTCATCATCTGGAAAGAGTTCAGTGACAAGTTTCCAGGGGTCTCCTGTATCTGCAGGAGGAGCTGAAAGTGATCTGATTGCAGTTCAACTTCAACACCAATTGAATAAAATTTCAAGTCAACAACATGCACCTTCTTCAGAGTCTCACAAAATTGTACGTGGTTCATATTTGTCGAGAAAACTGAGATGA
Protein sequence

>Msa0343770

MDDKNMKGEDGEVDIESGLAVTKDDSGNTKQERTLFAKIYCGIVGDSIKVEERNSILYLKDSNLSGDSVNVAKESNKLLTGQDLVKSVENSPVKEKRKKSGNKKAAKPPRAPRGPSLDAADQKLIREITQLAMLKRARVERMKALKKMKAAKASSSPSSSMFAMVCTVVFCIVILFQGMSSSGKSSVTSFQGSPVSAGGAESDLIAVQLQHQLNKISSQQHAPSSESHKIVRGSYLSRKLR*