AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1218920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1218920 MtrunA17_Chr4g0068671 95.021 241 12 0 1 241 1 241 2.40e-139 390
Msa1218920 MtrunA17_Chr2g0294131 55.285 246 81 7 1 241 1 222 1.63e-53 172
Msa1218920 MtrunA17_Chr1g0194401 40.120 167 95 4 67 232 25 187 2.78e-28 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1218920 AT1G02380.1 34.649 228 124 10 3 225 2 209 5.59e-28 107
Msa1218920 AT3G17120.4 43.478 161 57 6 71 208 30 179 2.05e-26 102
Msa1218920 AT3G17120.2 43.478 161 57 6 71 208 30 179 2.05e-26 102
Msa1218920 AT3G17120.1 43.478 161 57 6 71 208 30 179 2.05e-26 102
Msa1218920 AT3G17120.5 43.478 161 57 6 71 208 30 179 2.05e-26 102
Msa1218920 AT3G17120.3 43.478 161 57 6 71 208 30 179 2.05e-26 102
Msa1218920 AT4G01960.1 50.495 101 47 3 113 213 106 203 2.12e-23 95.1
Msa1218920 AT4G01960.2 50.495 101 47 3 113 213 106 203 2.12e-23 95.1
Msa1218920 AT4G01960.3 65.152 66 22 1 113 178 106 170 2.40e-20 85.9

Find 82 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTTTACATTGTGATTTA+AGG 0.098041 8_1:+81626909 Msa1218920:three_prime_UTR
CTGCTGATCAAAAGCTAATT+AGG 0.140099 8_1:+81625791 Msa1218920:CDS
TTAACCTAAACTACAAAATT+AGG 0.172537 8_1:-81625298 None:intergenic
TTTGTCCCCTCATCGGATCT+TGG 0.258976 8_1:+81624752 Msa1218920:five_prime_UTR
TGTAGTTTAGGTTAACAATC+AGG 0.318334 8_1:+81625306 Msa1218920:five_prime_UTR
GACATTGATATAAAATAATT+AGG 0.333160 8_1:-81627019 None:intergenic
GAGTTCAGTGACAAGTTTCC+AGG 0.338873 8_1:+81626511 Msa1218920:CDS
GACATTGATATAAAATAATT+AGG 0.339980 8_1:-81627058 None:intergenic
TTGTCCCCTCATCGGATCTT+GGG 0.368020 8_1:+81624753 Msa1218920:five_prime_UTR
GGCAGTAACTAAAGATGATT+CGG 0.374127 8_1:+81625492 Msa1218920:CDS
GTCTAAACTTCACCCTCATC+AGG 0.389569 8_1:-81626949 None:intergenic
TGTATTGTGATCTTATTTCA+AGG 0.410724 8_1:+81625946 Msa1218920:CDS
AGGGTGAAGTTTAGACTTGT+AGG 0.415952 8_1:+81626956 Msa1218920:three_prime_UTR
TGTAGAGAATAGTCCGGTTA+AGG 0.419669 8_1:+81625696 Msa1218920:CDS
TGCTGATCAAAAGCTAATTA+GGG 0.424444 8_1:+81625792 Msa1218920:CDS
CAAAACTAATAATTAAACTT+TGG 0.429207 8_1:-81625403 None:intergenic
AAGATTTATTGTGGTATTGT+TGG 0.429970 8_1:+81625547 Msa1218920:CDS
TACATTGTGATTTAAGGTGT+TGG 0.433731 8_1:+81626915 Msa1218920:three_prime_UTR
CAGAGAGATTTGAATGCAAC+AGG 0.435389 8_1:+81624670 Msa1218920:five_prime_UTR
TTTGCAGGCATGTCATCATC+TGG 0.437634 8_1:+81626485 Msa1218920:intron
ACATTGTGATTTAAGGTGTT+GGG 0.440893 8_1:+81626916 Msa1218920:three_prime_UTR
TCAGCTCCTCCTGCAGATAC+AGG 0.451996 8_1:-81626539 None:intergenic
CTAAACTACAAAATTAGGTT+AGG 0.452713 8_1:-81625293 None:intergenic
CAGGGGTCTCCTGTATCTGC+AGG 0.453931 8_1:+81626530 Msa1218920:CDS
GCAGTAACTAAAGATGATTC+GGG 0.461926 8_1:+81625493 Msa1218920:CDS
GGTGAAGTTGATATTGAAAG+TGG 0.477042 8_1:+81625466 Msa1218920:CDS
ATGGCAAACATGCTGCTGCT+TGG 0.493125 8_1:-81625904 None:intergenic
AGATTTACTGACAATAAAGA+TGG 0.495380 8_1:+81626834 Msa1218920:three_prime_UTR
TATGCTCTTTGTCCCCTCAT+CGG 0.500303 8_1:+81624745 Msa1218920:five_prime_UTR
GTTGTTGACGGGGCAGGACT+TGG 0.513107 8_1:+81625666 Msa1218920:CDS
GAGTCTAACAAGTTGTTGAC+GGG 0.513280 8_1:+81625655 Msa1218920:CDS
AGCTCCACGAGGGCCGTCGT+TGG 0.517134 8_1:+81625765 Msa1218920:CDS
GTAGTTTAGGTTAACAATCA+GGG 0.520089 8_1:+81625307 Msa1218920:five_prime_UTR
CACCCTCGCACGCTTCAACA+TGG 0.521089 8_1:-81625830 None:intergenic
ATAGTCCGGTTAAGGAGAAA+CGG 0.534537 8_1:+81625704 Msa1218920:CDS
TTGAAAGATTCAAATTTGAG+TGG 0.539571 8_1:+81625610 Msa1218920:CDS
AAGCAGCAGCATGTTTGCCA+TGG 0.544835 8_1:+81625906 Msa1218920:CDS
TAACAAGTTGTTGACGGGGC+AGG 0.545014 8_1:+81625660 Msa1218920:CDS
AATAATTAGGTGATTACAGA+TGG 0.545580 8_1:-81627006 None:intergenic
TTATTTGCTAAGATTTATTG+TGG 0.547930 8_1:+81625538 Msa1218920:CDS
CGGGTAATACTAAACAAGAA+AGG 0.549432 8_1:+81625512 Msa1218920:CDS
GACGGCCCTCGTGGAGCTCT+AGG 0.551220 8_1:-81625760 None:intergenic
GGAGAGAGAAGATGCTAGGA+AGG 0.551589 8_1:-81624578 None:intergenic
AGAAAACTGAGATGAGATGA+AGG 0.556919 8_1:+81626804 Msa1218920:exon
TAAATGTTCCTCTCACAAAG+AGG 0.559209 8_1:-81625345 None:intergenic
GGGTAATTTATCCCCTGATG+AGG 0.559462 8_1:+81626936 Msa1218920:three_prime_UTR
CACAATGTAAAGAAATACAT+TGG 0.560595 8_1:-81626901 None:intergenic
CATGTTGAAGCGTGCGAGGG+TGG 0.561704 8_1:+81625831 Msa1218920:CDS
TTAGGTTAACAATCAGGGTG+AGG 0.567911 8_1:+81625312 Msa1218920:five_prime_UTR
AGAGTCTAACAAGTTGTTGA+CGG 0.570293 8_1:+81625654 Msa1218920:CDS
GAAAAGTGTAGAGAATAGTC+CGG 0.572405 8_1:+81625690 Msa1218920:CDS
GTGAAGTTGATATTGAAAGT+GGG 0.572872 8_1:+81625467 Msa1218920:CDS
GGTAATTTATCCCCTGATGA+GGG 0.577082 8_1:+81626937 Msa1218920:three_prime_UTR
TCTAAACTTCACCCTCATCA+GGG 0.581373 8_1:-81626948 None:intergenic
AGAAGAGAACCACCGACAGT+TGG 0.582996 8_1:-81624553 None:intergenic
AGTTCAGTGACAAGTTTCCA+GGG 0.583334 8_1:+81626512 Msa1218920:CDS
GCATCCAACGACGGCCCTCG+TGG 0.589028 8_1:-81625769 None:intergenic
CTGCAGATACAGGAGACCCC+TGG 0.589034 8_1:-81626529 None:intergenic
TACTTGTGAGACTCTGAAGA+AGG 0.591671 8_1:-81626626 None:intergenic
GGGTCTCCTGTATCTGCAGG+AGG 0.593983 8_1:+81626533 Msa1218920:CDS
AGCTGGAGAGAGAAGATGCT+AGG 0.600356 8_1:-81624582 None:intergenic
TTGCCATGTTGAAGCGTGCG+AGG 0.607905 8_1:+81625827 Msa1218920:CDS
AGAGACGGACACTCACTCAC+AGG 0.610518 8_1:-81624722 None:intergenic
AGAAACCCAAGATCCGATGA+GGG 0.614811 8_1:-81624758 None:intergenic
AGTTGATATTGAAAGTGGGT+TGG 0.627313 8_1:+81625471 Msa1218920:CDS
AAGAAACCCAAGATCCGATG+AGG 0.644513 8_1:-81624759 None:intergenic
CTACCTTACAGAATTGTACG+TGG 0.646628 8_1:+81626768 Msa1218920:intron
GAGTGGAGATTCTGTGAATG+TGG 0.653592 8_1:+81625627 Msa1218920:CDS
TGATCAGCAGCATCCAACGA+CGG 0.653843 8_1:-81625778 None:intergenic
TGCCATGTTGAAGCGTGCGA+GGG 0.655794 8_1:+81625828 Msa1218920:CDS
CTAAACTTCACCCTCATCAG+GGG 0.659411 8_1:-81626947 None:intergenic
GGCCCTCGTGGAGCTCTAGG+AGG 0.662072 8_1:-81625757 None:intergenic
GGGCTTGTGTGAGATGCACA+AGG 0.666533 8_1:-81626865 None:intergenic
GCAGCAGTGTTGCCAACTGT+CGG 0.673083 8_1:+81624541 Msa1218920:five_prime_UTR
GAAACCCAAGATCCGATGAG+GGG 0.673655 8_1:-81624757 None:intergenic
GGGACAAAGAGCATAAGAGA+CGG 0.674570 8_1:-81624737 None:intergenic
GTTCAGTGACAAGTTTCCAG+GGG 0.676920 8_1:+81626513 Msa1218920:CDS
GAAGACAACAGTGCAAACCA+TGG 0.690412 8_1:-81625923 None:intergenic
AGTCTAACAAGTTGTTGACG+GGG 0.706378 8_1:+81625656 Msa1218920:CDS
GCAGTGTTGCCAACTGTCGG+TGG 0.713536 8_1:+81624544 Msa1218920:five_prime_UTR
AACCTCCTAGAGCTCCACGA+GGG 0.737863 8_1:+81625755 Msa1218920:CDS
AAACCTCCTAGAGCTCCACG+AGG 0.781040 8_1:+81625754 Msa1218920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAACTAATAATTAAACTT+TGG - chr8_1:81625406-81625425 None:intergenic 15.0%
!! GACATTGATATAAAATAATT+AGG - chr8_1:81627022-81627041 None:intergenic 15.0%
!! GACATTGATATAAAATAATT+AGG - chr8_1:81627022-81627041 None:intergenic 15.0%
!!! GTTTTATTGTAAATGAAAGA+AGG - chr8_1:81624803-81624822 None:intergenic 20.0%
!! AAAAAATGAATGACATTGAA+GGG - chr8_1:81624858-81624877 None:intergenic 20.0%
!! TATTGCTATGTTTAATTCAT+TGG + chr8_1:81624929-81624948 Msa1218920:intron 20.0%
!!! TGTCTGATATCATTTAAAAT+TGG - chr8_1:81625014-81625033 None:intergenic 20.0%
!!! TCTTTTTTTTCTACAATTTC+AGG + chr8_1:81625138-81625157 Msa1218920:intron 20.0%
!!! CTTTTTTTTCTACAATTTCA+GGG + chr8_1:81625139-81625158 Msa1218920:intron 20.0%
!!! TTTTTTTTCTACAATTTCAG+GGG + chr8_1:81625140-81625159 Msa1218920:intron 20.0%
!! AATTGCAAATGACAAAAAAA+TGG - chr8_1:81625225-81625244 None:intergenic 20.0%
!! TTAACCTAAACTACAAAATT+AGG - chr8_1:81625301-81625320 None:intergenic 20.0%
!!! AATTATTAGTTTTGTGAGTT+TGG + chr8_1:81625412-81625431 Msa1218920:five_prime_UTR 20.0%
!!! ATTATTAGTTTTGTGAGTTT+GGG + chr8_1:81625413-81625432 Msa1218920:five_prime_UTR 20.0%
!! ATGGATGATAAAAATATGAA+AGG + chr8_1:81625436-81625455 Msa1218920:CDS 20.0%
!! TGGATGATAAAAATATGAAA+GGG + chr8_1:81625437-81625456 Msa1218920:CDS 20.0%
!!! TTATTTGCTAAGATTTATTG+TGG + chr8_1:81625538-81625557 Msa1218920:CDS 20.0%
!! ACAATTGACAAATGATATAA+TGG + chr8_1:81626062-81626081 Msa1218920:intron 20.0%
!! TACTCAATCAATATGAAATT+CGG - chr8_1:81626307-81626326 None:intergenic 20.0%
!!! GACTTGAAATTTTATTCAAT+TGG - chr8_1:81626595-81626614 None:intergenic 20.0%
!! AACAATAAGAATATTACTAC+CGG - chr8_1:81626740-81626759 None:intergenic 20.0%
!! TTTCTTTACATTGTGATTTA+AGG + chr8_1:81626909-81626928 Msa1218920:three_prime_UTR 20.0%
! AAAAATGGGATTTATATAGC+TGG - chr8_1:81624602-81624621 None:intergenic 25.0%
! TCTGTGATTCTTCAAAAAAT+GGG - chr8_1:81624616-81624635 None:intergenic 25.0%
! ATCTGTGATTCTTCAAAAAA+TGG - chr8_1:81624617-81624636 None:intergenic 25.0%
! ATGAATGAAAGAAAGAAAGA+GGG - chr8_1:81624826-81624845 None:intergenic 25.0%
! AATGAATGAAAGAAAGAAAG+AGG - chr8_1:81624827-81624846 None:intergenic 25.0%
! GAAAAAATGAATGACATTGA+AGG - chr8_1:81624859-81624878 None:intergenic 25.0%
! TTAAATGATATCAGACAACT+CGG + chr8_1:81625017-81625036 Msa1218920:intron 25.0%
!!! TTTTTTTCTACAATTTCAGG+GGG + chr8_1:81625141-81625160 Msa1218920:intron 25.0%
!! AATGGTTTTCTACAAATTGA+GGG - chr8_1:81625207-81625226 None:intergenic 25.0%
!! AAATGGTTTTCTACAAATTG+AGG - chr8_1:81625208-81625227 None:intergenic 25.0%
! CTAAACTACAAAATTAGGTT+AGG - chr8_1:81625296-81625315 None:intergenic 25.0%
!! CTAACCTAATTTTGTAGTTT+AGG + chr8_1:81625294-81625313 Msa1218920:five_prime_UTR 25.0%
! GGATGATAAAAATATGAAAG+GGG + chr8_1:81625438-81625457 Msa1218920:CDS 25.0%
! AAGATTTATTGTGGTATTGT+TGG + chr8_1:81625547-81625566 Msa1218920:CDS 25.0%
! TTGAAAGATTCAAATTTGAG+TGG + chr8_1:81625610-81625629 Msa1218920:CDS 25.0%
! GAAAAAATCTGGTAACAAAA+AGG + chr8_1:81625726-81625745 Msa1218920:CDS 25.0%
!! TGTATTGTGATCTTATTTCA+AGG + chr8_1:81625946-81625965 Msa1218920:CDS 25.0%
! AATTAAAGTAGTATCTGTCA+TGG + chr8_1:81625979-81625998 Msa1218920:intron 25.0%
!! ATTGATTGAGTATTTTCAGA+AGG + chr8_1:81626315-81626334 Msa1218920:intron 25.0%
! CATAAACTTAGAGATAGTTA+TGG + chr8_1:81626351-81626370 Msa1218920:intron 25.0%
!! TTATAAGGTGTTAAATTTGC+AGG + chr8_1:81626470-81626489 Msa1218920:intron 25.0%
! GATTGTTCTTTATGAAATTG+AGG - chr8_1:81626705-81626724 None:intergenic 25.0%
! AGATTTACTGACAATAAAGA+TGG + chr8_1:81626834-81626853 Msa1218920:three_prime_UTR 25.0%
!!! TGACAATAAAGATGGTTTTA+CGG + chr8_1:81626842-81626861 Msa1218920:three_prime_UTR 25.0%
! TACATTGGAAGACAAAAAAA+GGG - chr8_1:81626889-81626908 None:intergenic 25.0%
! ATACATTGGAAGACAAAAAA+AGG - chr8_1:81626890-81626909 None:intergenic 25.0%
! CACAATGTAAAGAAATACAT+TGG - chr8_1:81626904-81626923 None:intergenic 25.0%
! AATAATTAGGTGATTACAGA+TGG - chr8_1:81627009-81627028 None:intergenic 25.0%
TGAATGAAAGAAAGAAAGAG+GGG - chr8_1:81624825-81624844 None:intergenic 30.0%
!! TTCTGTTAACTTGTAAGAAC+TGG - chr8_1:81625084-81625103 None:intergenic 30.0%
AGTTCTTACAAGTTAACAGA+AGG + chr8_1:81625083-81625102 Msa1218920:intron 30.0%
! ATGGTTTTCTACAAATTGAG+GGG - chr8_1:81625206-81625225 None:intergenic 30.0%
! TGTAGTTTAGGTTAACAATC+AGG + chr8_1:81625306-81625325 Msa1218920:five_prime_UTR 30.0%
! GTAGTTTAGGTTAACAATCA+GGG + chr8_1:81625307-81625326 Msa1218920:five_prime_UTR 30.0%
TGATAAAAATATGAAAGGGG+AGG + chr8_1:81625441-81625460 Msa1218920:CDS 30.0%
!! GTGAAGTTGATATTGAAAGT+GGG + chr8_1:81625467-81625486 Msa1218920:CDS 30.0%
TGCTGATCAAAAGCTAATTA+GGG + chr8_1:81625792-81625811 Msa1218920:CDS 30.0%
TCTGTATTGTCACATGATAT+AGG + chr8_1:81626024-81626043 Msa1218920:intron 30.0%
TTGTCACATGATATAGGTTT+AGG + chr8_1:81626030-81626049 Msa1218920:intron 30.0%
!! GGTATTGTTTTGTTACCTAT+TGG + chr8_1:81626122-81626141 Msa1218920:intron 30.0%
AAAACAGAACCAATAGGAAT+AGG - chr8_1:81626158-81626177 None:intergenic 30.0%
! ACTTTGAAAACAGAACCAAT+AGG - chr8_1:81626164-81626183 None:intergenic 30.0%
!!! TTGGTTCTGTTTTCAAAGTT+TGG + chr8_1:81626165-81626184 Msa1218920:intron 30.0%
AACAATCCTGAAATTTGGAA+TGG - chr8_1:81626191-81626210 None:intergenic 30.0%
GAAGTAACAATCCTGAAATT+TGG - chr8_1:81626196-81626215 None:intergenic 30.0%
GGCAAAAATTGTATGATTGA+TGG - chr8_1:81626387-81626406 None:intergenic 30.0%
! CAATTTTGCGTACTTTTACT+GGG - chr8_1:81626408-81626427 None:intergenic 30.0%
CCTTATAAATGAATACCACT+AGG - chr8_1:81626458-81626477 None:intergenic 30.0%
CCTAGTGGTATTCATTTATA+AGG + chr8_1:81626455-81626474 Msa1218920:intron 30.0%
GAAAATATGCTTGCAATGAA+AGG - chr8_1:81626670-81626689 None:intergenic 30.0%
ACATTGGAAGACAAAAAAAG+GGG - chr8_1:81626888-81626907 None:intergenic 30.0%
! TACATTGTGATTTAAGGTGT+TGG + chr8_1:81626915-81626934 Msa1218920:three_prime_UTR 30.0%
! ACATTGTGATTTAAGGTGTT+GGG + chr8_1:81626916-81626935 Msa1218920:three_prime_UTR 30.0%
! GTTTCTTCTCTTTTCGTCAA+AGG + chr8_1:81624775-81624794 Msa1218920:five_prime_UTR 35.0%
AACTTGTAAGAACTGGTTAC+TGG - chr8_1:81625077-81625096 None:intergenic 35.0%
CTCCAATTATGAATGACGTT+GGG - chr8_1:81625118-81625137 None:intergenic 35.0%
ACTCCAATTATGAATGACGT+TGG - chr8_1:81625119-81625138 None:intergenic 35.0%
! TGGTTTTCTACAAATTGAGG+GGG - chr8_1:81625205-81625224 None:intergenic 35.0%
!!! TCTGTCTTTTGTTTTGATGC+AGG + chr8_1:81625259-81625278 Msa1218920:intron 35.0%
!!! CTGTCTTTTGTTTTGATGCA+GGG + chr8_1:81625260-81625279 Msa1218920:intron 35.0%
AAAAATTGCCTCTTTGTGAG+AGG + chr8_1:81625337-81625356 Msa1218920:five_prime_UTR 35.0%
TAAATGTTCCTCTCACAAAG+AGG - chr8_1:81625348-81625367 None:intergenic 35.0%
!!! TTAGTTTTGTGAGTTTGGGA+TGG + chr8_1:81625417-81625436 Msa1218920:exon 35.0%
AAAAATATGAAAGGGGAGGA+TGG + chr8_1:81625445-81625464 Msa1218920:CDS 35.0%
!! GGTGAAGTTGATATTGAAAG+TGG + chr8_1:81625466-81625485 Msa1218920:CDS 35.0%
! AGTTGATATTGAAAGTGGGT+TGG + chr8_1:81625471-81625490 Msa1218920:CDS 35.0%
GGCAGTAACTAAAGATGATT+CGG + chr8_1:81625492-81625511 Msa1218920:CDS 35.0%
GCAGTAACTAAAGATGATTC+GGG + chr8_1:81625493-81625512 Msa1218920:CDS 35.0%
CGGGTAATACTAAACAAGAA+AGG + chr8_1:81625512-81625531 Msa1218920:CDS 35.0%
AGAGTCTAACAAGTTGTTGA+CGG + chr8_1:81625654-81625673 Msa1218920:CDS 35.0%
! GAAAAGTGTAGAGAATAGTC+CGG + chr8_1:81625690-81625709 Msa1218920:CDS 35.0%
! TTTTTCCGTTTCTCCTTAAC+CGG - chr8_1:81625712-81625731 None:intergenic 35.0%
AAGGAGAAACGGAAAAAATC+TGG + chr8_1:81625715-81625734 Msa1218920:CDS 35.0%
CTGCTGATCAAAAGCTAATT+AGG + chr8_1:81625791-81625810 Msa1218920:CDS 35.0%
! TGTTACTTCTGTCCTTTGTT+TGG + chr8_1:81626207-81626226 Msa1218920:intron 35.0%
! CCAATTTTGCGTACTTTTAC+TGG - chr8_1:81626409-81626428 None:intergenic 35.0%
! CCAGTAAAAGTACGCAAAAT+TGG + chr8_1:81626406-81626425 Msa1218920:intron 35.0%
AACAATCTCGATGTGATAAC+CGG + chr8_1:81626718-81626737 Msa1218920:intron 35.0%
AGAAAACTGAGATGAGATGA+AGG + chr8_1:81626804-81626823 Msa1218920:exon 35.0%
CAGAGAGATTTGAATGCAAC+AGG + chr8_1:81624670-81624689 Msa1218920:five_prime_UTR 40.0%
CGTCTATTCGCAAATTTCAG+GGG - chr8_1:81624991-81625010 None:intergenic 40.0%
GCGTCTATTCGCAAATTTCA+GGG - chr8_1:81624992-81625011 None:intergenic 40.0%
TGCCCAACGTCATTCATAAT+TGG + chr8_1:81625113-81625132 Msa1218920:intron 40.0%
TCTACAATTTCAGGGGGAAT+TGG + chr8_1:81625147-81625166 Msa1218920:intron 40.0%
ACAAATTGAGGGGGTAACTA+GGG - chr8_1:81625196-81625215 None:intergenic 40.0%
TACAAATTGAGGGGGTAACT+AGG - chr8_1:81625197-81625216 None:intergenic 40.0%
TTAGGTTAACAATCAGGGTG+AGG + chr8_1:81625312-81625331 Msa1218920:five_prime_UTR 40.0%
GAGTCTAACAAGTTGTTGAC+GGG + chr8_1:81625655-81625674 Msa1218920:CDS 40.0%
AGTCTAACAAGTTGTTGACG+GGG + chr8_1:81625656-81625675 Msa1218920:CDS 40.0%
TGTAGAGAATAGTCCGGTTA+AGG + chr8_1:81625696-81625715 Msa1218920:CDS 40.0%
ATAGTCCGGTTAAGGAGAAA+CGG + chr8_1:81625704-81625723 Msa1218920:CDS 40.0%
ACTGTTGCAAGTTGTTGAGT+TGG + chr8_1:81626100-81626119 Msa1218920:intron 40.0%
CTGTTGCAAGTTGTTGAGTT+GGG + chr8_1:81626101-81626120 Msa1218920:intron 40.0%
ATAGGTGGAAATGGACCAAT+AGG - chr8_1:81626140-81626159 None:intergenic 40.0%
CCAATAGGAATAGGTGGAAA+TGG - chr8_1:81626149-81626168 None:intergenic 40.0%
CCATTTCCACCTATTCCTAT+TGG + chr8_1:81626146-81626165 Msa1218920:intron 40.0%
ACAGAACCAATAGGAATAGG+TGG - chr8_1:81626155-81626174 None:intergenic 40.0%
GTTTGGCCATTCCAAATTTC+AGG + chr8_1:81626182-81626201 Msa1218920:intron 40.0%
! TACTTCTGTCCTTTGTTTGG+TGG + chr8_1:81626210-81626229 Msa1218920:intron 40.0%
TTGATGGAACCACCAAACAA+AGG - chr8_1:81626222-81626241 None:intergenic 40.0%
TAGAACTCACTCACATGTCA+TGG + chr8_1:81626260-81626279 Msa1218920:intron 40.0%
TGAGAATAATGCACTCCTAG+TGG + chr8_1:81626440-81626459 Msa1218920:intron 40.0%
AGTTCAGTGACAAGTTTCCA+GGG + chr8_1:81626512-81626531 Msa1218920:CDS 40.0%
! TACTTGTGAGACTCTGAAGA+AGG - chr8_1:81626629-81626648 None:intergenic 40.0%
CACAAGTAAGTAGAGATGCA+AGG + chr8_1:81626641-81626660 Msa1218920:intron 40.0%
CTACCTTACAGAATTGTACG+TGG + chr8_1:81626768-81626787 Msa1218920:intron 40.0%
GAACCACGTACAATTCTGTA+AGG - chr8_1:81626774-81626793 None:intergenic 40.0%
GGTAATTTATCCCCTGATGA+GGG + chr8_1:81626937-81626956 Msa1218920:three_prime_UTR 40.0%
TCTAAACTTCACCCTCATCA+GGG - chr8_1:81626951-81626970 None:intergenic 40.0%
AGGGTGAAGTTTAGACTTGT+AGG + chr8_1:81626956-81626975 Msa1218920:three_prime_UTR 40.0%
! GGGACAAAGAGCATAAGAGA+CGG - chr8_1:81624740-81624759 None:intergenic 45.0%
TATGCTCTTTGTCCCCTCAT+CGG + chr8_1:81624745-81624764 Msa1218920:five_prime_UTR 45.0%
AGAAACCCAAGATCCGATGA+GGG - chr8_1:81624761-81624780 None:intergenic 45.0%
AAGAAACCCAAGATCCGATG+AGG - chr8_1:81624762-81624781 None:intergenic 45.0%
!! TTTAGCTCGCCAATTTGCTG+TGG + chr8_1:81624952-81624971 Msa1218920:intron 45.0%
AATGTCCGACCACAGCAAAT+TGG - chr8_1:81624964-81624983 None:intergenic 45.0%
GGCGTCTATTCGCAAATTTC+AGG - chr8_1:81624993-81625012 None:intergenic 45.0%
ACTCGGTCCTTCTGTTAACT+TGG + chr8_1:81625034-81625053 Msa1218920:intron 45.0%
CTCGGTCCTTCTGTTAACTT+GGG + chr8_1:81625035-81625054 Msa1218920:intron 45.0%
! TCCTTCTGTTAACTTGGGCA+GGG + chr8_1:81625040-81625059 Msa1218920:intron 45.0%
GAGTGGAGATTCTGTGAATG+TGG + chr8_1:81625627-81625646 Msa1218920:CDS 45.0%
GAAGACAACAGTGCAAACCA+TGG - chr8_1:81625926-81625945 None:intergenic 45.0%
TTTGCAGGCATGTCATCATC+TGG + chr8_1:81626485-81626504 Msa1218920:intron 45.0%
GAGTTCAGTGACAAGTTTCC+AGG + chr8_1:81626511-81626530 Msa1218920:CDS 45.0%
GTTCAGTGACAAGTTTCCAG+GGG + chr8_1:81626513-81626532 Msa1218920:CDS 45.0%
GGGTAATTTATCCCCTGATG+AGG + chr8_1:81626936-81626955 Msa1218920:three_prime_UTR 45.0%
CTAAACTTCACCCTCATCAG+GGG - chr8_1:81626950-81626969 None:intergenic 45.0%
GTCTAAACTTCACCCTCATC+AGG - chr8_1:81626952-81626971 None:intergenic 45.0%
!!! AAAATAATAATAAATTTTGA+TGG - chr8_1:81626238-81626257 None:intergenic 5.0%
!!! ATCAAAATTTATTATTATTT+TGG + chr8_1:81626237-81626256 Msa1218920:intron 5.0%
AGAAGAGAACCACCGACAGT+TGG - chr8_1:81624556-81624575 None:intergenic 50.0%
GGAGAGAGAAGATGCTAGGA+AGG - chr8_1:81624581-81624600 None:intergenic 50.0%
AGCTGGAGAGAGAAGATGCT+AGG - chr8_1:81624585-81624604 None:intergenic 50.0%
TTTGTCCCCTCATCGGATCT+TGG + chr8_1:81624752-81624771 Msa1218920:five_prime_UTR 50.0%
TTGTCCCCTCATCGGATCTT+GGG + chr8_1:81624753-81624772 Msa1218920:five_prime_UTR 50.0%
GAAACCCAAGATCCGATGAG+GGG - chr8_1:81624760-81624779 None:intergenic 50.0%
! GTCCTTCTGTTAACTTGGGC+AGG + chr8_1:81625039-81625058 Msa1218920:intron 50.0%
TAACAAGTTGTTGACGGGGC+AGG + chr8_1:81625660-81625679 Msa1218920:CDS 50.0%
TGATCAGCAGCATCCAACGA+CGG - chr8_1:81625781-81625800 None:intergenic 50.0%
ATGGCAAACATGCTGCTGCT+TGG - chr8_1:81625907-81625926 None:intergenic 50.0%
AAGCAGCAGCATGTTTGCCA+TGG + chr8_1:81625906-81625925 Msa1218920:CDS 50.0%
GCAGCAGTGTTGCCAACTGT+CGG + chr8_1:81624541-81624560 Msa1218920:five_prime_UTR 55.0%
AGAGACGGACACTCACTCAC+AGG - chr8_1:81624725-81624744 None:intergenic 55.0%
!! GCTCGCCAATTTGCTGTGGT+CGG + chr8_1:81624956-81624975 Msa1218920:intron 55.0%
GCCCTGCCCAAGTTAACAGA+AGG - chr8_1:81625044-81625063 None:intergenic 55.0%
AAACCTCCTAGAGCTCCACG+AGG + chr8_1:81625754-81625773 Msa1218920:CDS 55.0%
AACCTCCTAGAGCTCCACGA+GGG + chr8_1:81625755-81625774 Msa1218920:CDS 55.0%
TTGCCATGTTGAAGCGTGCG+AGG + chr8_1:81625827-81625846 Msa1218920:CDS 55.0%
TGCCATGTTGAAGCGTGCGA+GGG + chr8_1:81625828-81625847 Msa1218920:CDS 55.0%
TCAGCTCCTCCTGCAGATAC+AGG - chr8_1:81626542-81626561 None:intergenic 55.0%
GGGCTTGTGTGAGATGCACA+AGG - chr8_1:81626868-81626887 None:intergenic 55.0%
GCAGTGTTGCCAACTGTCGG+TGG + chr8_1:81624544-81624563 Msa1218920:five_prime_UTR 60.0%
GTTGTTGACGGGGCAGGACT+TGG + chr8_1:81625666-81625685 Msa1218920:CDS 60.0%
CACCCTCGCACGCTTCAACA+TGG - chr8_1:81625833-81625852 None:intergenic 60.0%
CATGTTGAAGCGTGCGAGGG+TGG + chr8_1:81625831-81625850 Msa1218920:CDS 60.0%
CTGCAGATACAGGAGACCCC+TGG - chr8_1:81626532-81626551 None:intergenic 60.0%
CAGGGGTCTCCTGTATCTGC+AGG + chr8_1:81626530-81626549 Msa1218920:CDS 60.0%
GGGTCTCCTGTATCTGCAGG+AGG + chr8_1:81626533-81626552 Msa1218920:CDS 60.0%
!! GGCCCTCGTGGAGCTCTAGG+AGG - chr8_1:81625760-81625779 None:intergenic 70.0%
GACGGCCCTCGTGGAGCTCT+AGG - chr8_1:81625763-81625782 None:intergenic 70.0%
AGCTCCACGAGGGCCGTCGT+TGG + chr8_1:81625765-81625784 Msa1218920:CDS 70.0%
GCATCCAACGACGGCCCTCG+TGG - chr8_1:81625772-81625791 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 81624503 81627114 81624503 ID=Msa1218920;Name=Msa1218920
chr8_1 mRNA 81624503 81627114 81624503 ID=Msa1218920-mRNA-1;Parent=Msa1218920;Name=Msa1218920-mRNA-1;_AED=0.01;_eAED=0.01;_QI=449|1|1|1|0.66|0.5|4|296|241
chr8_1 exon 81624503 81624796 81624503 ID=Msa1218920-mRNA-1:exon:28905;Parent=Msa1218920-mRNA-1
chr8_1 exon 81625281 81625967 81625281 ID=Msa1218920-mRNA-1:exon:28906;Parent=Msa1218920-mRNA-1
chr8_1 exon 81626492 81626645 81626492 ID=Msa1218920-mRNA-1:exon:28907;Parent=Msa1218920-mRNA-1
chr8_1 exon 81626779 81627114 81626779 ID=Msa1218920-mRNA-1:exon:28908;Parent=Msa1218920-mRNA-1
chr8_1 five_prime_UTR 81624503 81624796 81624503 ID=Msa1218920-mRNA-1:five_prime_utr;Parent=Msa1218920-mRNA-1
chr8_1 five_prime_UTR 81625281 81625435 81625281 ID=Msa1218920-mRNA-1:five_prime_utr;Parent=Msa1218920-mRNA-1
chr8_1 CDS 81625436 81625967 81625436 ID=Msa1218920-mRNA-1:cds;Parent=Msa1218920-mRNA-1
chr8_1 CDS 81626492 81626645 81626492 ID=Msa1218920-mRNA-1:cds;Parent=Msa1218920-mRNA-1
chr8_1 CDS 81626779 81626818 81626779 ID=Msa1218920-mRNA-1:cds;Parent=Msa1218920-mRNA-1
chr8_1 three_prime_UTR 81626819 81627114 81626819 ID=Msa1218920-mRNA-1:three_prime_utr;Parent=Msa1218920-mRNA-1
Gene Sequence

>Msa1218920

ATGGATGATAAAAATATGAAAGGGGAGGATGGTGAAGTTGATATTGAAAGTGGGTTGGCAGTAACTAAAGATGATTCGGGTAATACTAAACAAGAAAGGACATTATTTGCTAAGATTTATTGTGGTATTGTTGGAGATTCTATAAAAGTTGAAGAAAGAAATAGTATATTATACTTGAAAGATTCAAATTTGAGTGGAGATTCTGTGAATGTGGCGAAAGAGTCTAACAAGTTGTTGACGGGGCAGGACTTGGTGAAAAGTGTAGAGAATAGTCCGGTTAAGGAGAAACGGAAAAAATCTGGTAACAAAAAGGCTGCTAAACCTCCTAGAGCTCCACGAGGGCCGTCGTTGGATGCTGCTGATCAAAAGCTAATTAGGGAGATTACTCAACTTGCCATGTTGAAGCGTGCGAGGGTGGAGCGTATGAAAGCTTTGAAAAAGATGAAAGCTGCTAAAGCATCTTCGTCTCCAAGCAGCAGCATGTTTGCCATGGTTTGCACTGTTGTCTTCTGTATTGTGATCTTATTTCAAGGCATGTCATCATCTGGAAAGAGTTCAGTGACAAGTTTCCAGGGGTCTCCTGTATCTGCAGGAGGAGCTGAAAGTGATCTGATTGCAGTTCAACTTCAACACCAATTGAATAAAATTTCAAGTCAACAACATGCACCTTCTTCAGAGTCTCACAAAATTGTACGTGGTTCATATTTGTCGAGAAAACTGAGATGA
Protein sequence

>Msa1218920

MDDKNMKGEDGEVDIESGLAVTKDDSGNTKQERTLFAKIYCGIVGDSIKVEERNSILYLKDSNLSGDSVNVAKESNKLLTGQDLVKSVENSPVKEKRKKSGNKKAAKPPRAPRGPSLDAADQKLIREITQLAMLKRARVERMKALKKMKAAKASSSPSSSMFAMVCTVVFCIVILFQGMSSSGKSSVTSFQGSPVSAGGAESDLIAVQLQHQLNKISSQQHAPSSESHKIVRGSYLSRKLR*