AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1334050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 76 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACTTGGGTGGTGTGGGAAT+AGG 0.179418 8_4:-54357407 Msa1334050:CDS
GCCGGTGCAGGGTCTGGTTC+AGG 0.215595 8_4:-54357329 Msa1334050:CDS
TCTGGTTCGGGTTCTGGTTC+TGG 0.216048 8_4:-54357305 Msa1334050:intron
TCAGGGTCTGGTTCGGGTTC+TGG 0.223414 8_4:-54357311 Msa1334050:CDS
TCTATTTCTTGCATGTAATA+AGG 0.237929 8_4:-54356853 Msa1334050:three_prime_UTR
ACTTGGGTGGTGTGGGAATA+GGG 0.241823 8_4:-54357406 Msa1334050:CDS
GAACTTTATTATAATATATA+TGG 0.256935 8_4:+54356999 None:intergenic
TCTCGTGCAGGGTCAGGGGC+TGG 0.277683 8_4:-54357218 Msa1334050:CDS
GGTGGTGGTGGTGCTGGCTC+TGG 0.298903 8_4:-54357353 Msa1334050:CDS
GCTGGCTCTGGTGCCGGTGC+AGG 0.301841 8_4:-54357341 Msa1334050:CDS
ACGTTACCCTCTCTTGTTTA+GGG 0.303893 8_4:-54356822 Msa1334050:three_prime_UTR
GTCTGGTTCAGGGTCTGGTT+CGG 0.312395 8_4:-54357318 Msa1334050:CDS
TCTGGTTCAGGGTCTGGTTC+GGG 0.325239 8_4:-54357317 Msa1334050:CDS
TACGTTACCCTCTCTTGTTT+AGG 0.327543 8_4:-54356823 Msa1334050:three_prime_UTR
GCAGGATCTCGTGCAGGGTC+AGG 0.327834 8_4:-54357224 Msa1334050:CDS
GCAGGGTCTCATGCTGGGTC+AGG 0.339790 8_4:-54357095 Msa1334050:intron
ATAGGGTTGGGTGCTGGTCT+AGG 0.348841 8_4:-54357389 Msa1334050:CDS
GCAGGGTCTGGTTCAGGGTC+TGG 0.350841 8_4:-54357323 Msa1334050:CDS
TCTCATGCTGGGTCAGGGTC+TGG 0.360214 8_4:-54357089 Msa1334050:CDS
GGATCGGGATCAGGGTCTTC+TGG 0.361785 8_4:-54357272 Msa1334050:CDS
CTCGTGCAGGGTCAGGGGCT+GGG 0.364749 8_4:-54357217 Msa1334050:CDS
GCAGGGTCAGGGGCTGGGTC+AGG 0.366905 8_4:-54357212 Msa1334050:CDS
TCAGGGTCTGGTTCTGAAGC+TGG 0.377186 8_4:-54357077 Msa1334050:CDS
TCATATGCAGGGTCTCATGC+TGG 0.384968 8_4:-54357101 Msa1334050:intron
TCTGGTGCCGGTGCAGGGTC+TGG 0.387411 8_4:-54357335 Msa1334050:CDS
AAAGGACCTTGGGTTGGACT+TGG 0.389761 8_4:-54357423 Msa1334050:CDS
TTGGGTGCTGGTCTAGGACT+TGG 0.390919 8_4:-54357383 Msa1334050:CDS
TCTGCTTCAGGATCGGGATC+AGG 0.391270 8_4:-54357281 Msa1334050:intron
TGGGTTGGACTTGGGTGGTG+TGG 0.397876 8_4:-54357414 Msa1334050:CDS
CTTGGACTTGGTGGTGGTGG+TGG 0.399016 8_4:-54357365 Msa1334050:CDS
GGGTTGGACTTGGGTGGTGT+GGG 0.411811 8_4:-54357413 Msa1334050:CDS
CCGGTGCAGGGTCTGGTTCA+GGG 0.417735 8_4:-54357328 Msa1334050:CDS
CAGGATCTCGTGCAGGGTCA+GGG 0.422958 8_4:-54357223 Msa1334050:CDS
GGGTGGTGTGGGAATAGGGT+TGG 0.424884 8_4:-54357402 Msa1334050:CDS
GTGGGAATAGGGTTGGGTGC+TGG 0.428348 8_4:-54357395 Msa1334050:CDS
TCTGAAGCTGGTTCATATGC+AGG 0.430821 8_4:-54357065 Msa1334050:CDS
GGTGGTGCTGGCTCTGGTGC+CGG 0.433528 8_4:-54357347 Msa1334050:CDS
GCTGGTCTAGGACTTGGACT+TGG 0.437253 8_4:-54357377 Msa1334050:CDS
GGACTTGGACTTGGTGGTGG+TGG 0.442166 8_4:-54357368 Msa1334050:CDS
CTGGCTCTGGTGCCGGTGCA+GGG 0.443610 8_4:-54357340 Msa1334050:CDS
GCAGGGTCCCATGCTGGATC+AGG 0.455555 8_4:-54357047 Msa1334050:CDS
TCATATGCAGGGTCCCATGC+TGG 0.457998 8_4:-54357053 Msa1334050:CDS
TCTCATGCAGGATCTCGTGC+AGG 0.458538 8_4:-54357230 Msa1334050:CDS
CTAGGACTTGGACTTGGTGG+TGG 0.462464 8_4:-54357371 Msa1334050:CDS
GGATCAGGGTCTTCTGGAGC+TGG 0.463140 8_4:-54357266 Msa1334050:CDS
TCTGAAGCCGGTTCATATGC+AGG 0.467588 8_4:-54357182 Msa1334050:intron
TGAAAGTAGAGCAGCGAGAA+AGG 0.468232 8_4:-54357441 Msa1334050:CDS
GGTGGTGTGGGAATAGGGTT+GGG 0.480105 8_4:-54357401 Msa1334050:CDS
GAGCAGCGAGAAAGGACCTT+GGG 0.484360 8_4:-54357433 Msa1334050:CDS
CAGGGTCTCATGCTGGGTCA+GGG 0.484638 8_4:-54357094 Msa1334050:CDS
GGTCTAGGACTTGGACTTGG+TGG 0.486657 8_4:-54357374 Msa1334050:CDS
TCTGGAGCTGGTTCTGAAGC+CGG 0.491049 8_4:-54357254 Msa1334050:CDS
AAGGACCTTGGGTTGGACTT+GGG 0.494177 8_4:-54357422 Msa1334050:CDS
ATGAGAACCTGCATATGAAC+CGG 0.496137 8_4:+54357175 None:intergenic
TCTGAAGCCGGCTCTCATGC+AGG 0.510342 8_4:-54357242 Msa1334050:CDS
CATATGCAGGGTCTCATGCT+GGG 0.511139 8_4:-54357100 Msa1334050:intron
ATTACATGCAAGAAATAGAA+AGG 0.511275 8_4:+54356857 None:intergenic
CTGCTTCAGGATCGGGATCA+GGG 0.517642 8_4:-54357280 Msa1334050:intron
TGGACACATGCATATATAAG+AGG 0.528813 8_4:+54356899 None:intergenic
GACCTTGGGTTGGACTTGGG+TGG 0.531909 8_4:-54357419 Msa1334050:CDS
TCCCATGCTGGATCAGGAAG+TGG 0.533422 8_4:-54357041 Msa1334050:CDS
CGTTACCCTCTCTTGTTTAG+GGG 0.542020 8_4:-54356821 Msa1334050:three_prime_UTR
CCCTGAACCAGACCCTGCAC+CGG 0.544836 8_4:+54357328 None:intergenic
AGGATCTCGTGCAGGGTCAG+GGG 0.549900 8_4:-54357222 Msa1334050:CDS
TTCCACTTCCTGATCCAGCA+TGG 0.553357 8_4:+54357039 None:intergenic
TCAGGAGCAGATTCTGAAGC+CGG 0.558363 8_4:-54357194 Msa1334050:CDS
TCCACTTCCTGATCCAGCAT+GGG 0.559462 8_4:+54357040 None:intergenic
ACGAGATCCTGCATGAGAGC+CGG 0.567620 8_4:+54357235 None:intergenic
CTGAAGCTGGTTCATATGCA+GGG 0.570910 8_4:-54357064 Msa1334050:CDS
AGAGCAGCGAGAAAGGACCT+TGG 0.580493 8_4:-54357434 Msa1334050:CDS
GTTACCCTCTCTTGTTTAGG+GGG 0.598875 8_4:-54356820 Msa1334050:three_prime_UTR
AGCGAGAAAGGACCTTGGGT+TGG 0.600015 8_4:-54357429 Msa1334050:CDS
CTCATGCAGGATCTCGTGCA+GGG 0.622416 8_4:-54357229 Msa1334050:CDS
CACCACCCAAGTCCAACCCA+AGG 0.633824 8_4:+54357417 None:intergenic
TAATCATCATCAGATAGTGA+AGG 0.660205 8_4:+54356975 None:intergenic
GAAGAAACAATATTACACGA+TGG 0.686822 8_4:+54356879 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAACTTTATTATAATATATA+TGG + chr8_4:54357251-54357270 None:intergenic 10.0%
!! GAACTTTATTATAATATATA+TGG + chr8_4:54357251-54357270 None:intergenic 10.0%
!!! TATGCATATTACATTTTGAT+TGG + chr8_4:54357476-54357495 None:intergenic 20.0%
!!! TATGCATATTACATTTTGAT+TGG + chr8_4:54357476-54357495 None:intergenic 20.0%
! ATTACATGCAAGAAATAGAA+AGG + chr8_4:54357393-54357412 None:intergenic 25.0%
!! TCTATTTCTTGCATGTAATA+AGG - chr8_4:54357394-54357413 Msa1334050:CDS 25.0%
! ATTACATGCAAGAAATAGAA+AGG + chr8_4:54357393-54357412 None:intergenic 25.0%
!! TCTATTTCTTGCATGTAATA+AGG - chr8_4:54357394-54357413 Msa1334050:CDS 25.0%
TAATCATCATCAGATAGTGA+AGG + chr8_4:54357275-54357294 None:intergenic 30.0%
GAAGAAACAATATTACACGA+TGG + chr8_4:54357371-54357390 None:intergenic 30.0%
TAATCATCATCAGATAGTGA+AGG + chr8_4:54357275-54357294 None:intergenic 30.0%
GAAGAAACAATATTACACGA+TGG + chr8_4:54357371-54357390 None:intergenic 30.0%
TGGACACATGCATATATAAG+AGG + chr8_4:54357351-54357370 None:intergenic 35.0%
TGGACACATGCATATATAAG+AGG + chr8_4:54357351-54357370 None:intergenic 35.0%
ATGAGAACCTGCATATGAAC+CGG + chr8_4:54357075-54357094 None:intergenic 40.0%
! TACGTTACCCTCTCTTGTTT+AGG - chr8_4:54357424-54357443 Msa1334050:CDS 40.0%
! ACGTTACCCTCTCTTGTTTA+GGG - chr8_4:54357425-54357444 Msa1334050:CDS 40.0%
ATTACCCCCTAAACAAGAGA+GGG + chr8_4:54357434-54357453 None:intergenic 40.0%
AATTACCCCCTAAACAAGAG+AGG + chr8_4:54357435-54357454 None:intergenic 40.0%
ATGAGAACCTGCATATGAAC+CGG + chr8_4:54357075-54357094 None:intergenic 40.0%
! TACGTTACCCTCTCTTGTTT+AGG - chr8_4:54357424-54357443 Msa1334050:CDS 40.0%
! ACGTTACCCTCTCTTGTTTA+GGG - chr8_4:54357425-54357444 Msa1334050:CDS 40.0%
ATTACCCCCTAAACAAGAGA+GGG + chr8_4:54357434-54357453 None:intergenic 40.0%
AATTACCCCCTAAACAAGAG+AGG + chr8_4:54357435-54357454 None:intergenic 40.0%
TGAAAGTAGAGCAGCGAGAA+AGG - chr8_4:54356806-54356825 Msa1334050:three_prime_UTR 45.0%
!! TTCTGGTTCTGCTTCAGGAT+CGG - chr8_4:54356959-54356978 Msa1334050:three_prime_UTR 45.0%
TCATATGCAGGTTCTCATGC+TGG - chr8_4:54357077-54357096 Msa1334050:CDS 45.0%
CATATGCAGGTTCTCATGCT+GGG - chr8_4:54357078-54357097 Msa1334050:CDS 45.0%
! ATATGAACCGGCTTCAGAAC+CGG + chr8_4:54357132-54357151 None:intergenic 45.0%
ATGAGACCCTGCATATGAAC+CGG + chr8_4:54357144-54357163 None:intergenic 45.0%
! TCTGAAGCTGGTTCATATGC+AGG - chr8_4:54357182-54357201 Msa1334050:intron 45.0%
! CTGAAGCTGGTTCATATGCA+GGG - chr8_4:54357183-54357202 Msa1334050:CDS 45.0%
! CGTTACCCTCTCTTGTTTAG+GGG - chr8_4:54357426-54357445 Msa1334050:CDS 45.0%
! GTTACCCTCTCTTGTTTAGG+GGG - chr8_4:54357427-54357446 Msa1334050:CDS 45.0%
TGAAAGTAGAGCAGCGAGAA+AGG - chr8_4:54356806-54356825 Msa1334050:three_prime_UTR 45.0%
!! TTCTGGTTCTGCTTCAGGAT+CGG - chr8_4:54356959-54356978 Msa1334050:three_prime_UTR 45.0%
TCATATGCAGGTTCTCATGC+TGG - chr8_4:54357077-54357096 Msa1334050:CDS 45.0%
CATATGCAGGTTCTCATGCT+GGG - chr8_4:54357078-54357097 Msa1334050:CDS 45.0%
! ATATGAACCGGCTTCAGAAC+CGG + chr8_4:54357132-54357151 None:intergenic 45.0%
ATGAGACCCTGCATATGAAC+CGG + chr8_4:54357144-54357163 None:intergenic 45.0%
! TCTGAAGCTGGTTCATATGC+AGG - chr8_4:54357182-54357201 Msa1334050:intron 45.0%
! CTGAAGCTGGTTCATATGCA+GGG - chr8_4:54357183-54357202 Msa1334050:CDS 45.0%
! CGTTACCCTCTCTTGTTTAG+GGG - chr8_4:54357426-54357445 Msa1334050:CDS 45.0%
! GTTACCCTCTCTTGTTTAGG+GGG - chr8_4:54357427-54357446 Msa1334050:CDS 45.0%
!! AAAGGACCTTGGGTTGGACT+TGG - chr8_4:54356824-54356843 Msa1334050:three_prime_UTR 50.0%
!! AAGGACCTTGGGTTGGACTT+GGG - chr8_4:54356825-54356844 Msa1334050:three_prime_UTR 50.0%
ACTTGGGTGGTGTGGGAATA+GGG - chr8_4:54356841-54356860 Msa1334050:three_prime_UTR 50.0%
!! TCTGGTTCTGGTTCTGCTTC+AGG - chr8_4:54356954-54356973 Msa1334050:three_prime_UTR 50.0%
!! TCTGGTTCTGCTTCAGGATC+GGG - chr8_4:54356960-54356979 Msa1334050:three_prime_UTR 50.0%
!! TCAGGAGCAGATTCTGAAGC+CGG - chr8_4:54357053-54357072 Msa1334050:CDS 50.0%
! TCTGAAGCCGGTTCATATGC+AGG - chr8_4:54357065-54357084 Msa1334050:CDS 50.0%
! TCTGAAGCCGGTTCATATGC+AGG - chr8_4:54357065-54357084 Msa1334050:CDS 50.0%
! CTGAAGCCGGTTCATATGCA+GGG - chr8_4:54357066-54357085 Msa1334050:CDS 50.0%
TCATATGCAGGGTCTCATGC+TGG - chr8_4:54357146-54357165 Msa1334050:intron 50.0%
CATATGCAGGGTCTCATGCT+GGG - chr8_4:54357147-54357166 Msa1334050:intron 50.0%
TCCACTTCCTGATCCAGCAT+GGG + chr8_4:54357210-54357229 None:intergenic 50.0%
TTCCACTTCCTGATCCAGCA+TGG + chr8_4:54357211-54357230 None:intergenic 50.0%
!! AAAGGACCTTGGGTTGGACT+TGG - chr8_4:54356824-54356843 Msa1334050:three_prime_UTR 50.0%
!! AAGGACCTTGGGTTGGACTT+GGG - chr8_4:54356825-54356844 Msa1334050:three_prime_UTR 50.0%
ACTTGGGTGGTGTGGGAATA+GGG - chr8_4:54356841-54356860 Msa1334050:three_prime_UTR 50.0%
!! TCTGGTTCTGGTTCTGCTTC+AGG - chr8_4:54356954-54356973 Msa1334050:three_prime_UTR 50.0%
!! TCTGGTTCTGCTTCAGGATC+GGG - chr8_4:54356960-54356979 Msa1334050:three_prime_UTR 50.0%
!! TCAGGAGCAGATTCTGAAGC+CGG - chr8_4:54357053-54357072 Msa1334050:CDS 50.0%
! TCTGAAGCCGGTTCATATGC+AGG - chr8_4:54357065-54357084 Msa1334050:CDS 50.0%
! TCTGAAGCCGGTTCATATGC+AGG - chr8_4:54357065-54357084 Msa1334050:CDS 50.0%
! CTGAAGCCGGTTCATATGCA+GGG - chr8_4:54357066-54357085 Msa1334050:CDS 50.0%
TCATATGCAGGGTCTCATGC+TGG - chr8_4:54357146-54357165 Msa1334050:intron 50.0%
CATATGCAGGGTCTCATGCT+GGG - chr8_4:54357147-54357166 Msa1334050:intron 50.0%
TCCACTTCCTGATCCAGCAT+GGG + chr8_4:54357210-54357229 None:intergenic 50.0%
TTCCACTTCCTGATCCAGCA+TGG + chr8_4:54357211-54357230 None:intergenic 50.0%
AGAGCAGCGAGAAAGGACCT+TGG - chr8_4:54356813-54356832 Msa1334050:three_prime_UTR 55.0%
GAGCAGCGAGAAAGGACCTT+GGG - chr8_4:54356814-54356833 Msa1334050:three_prime_UTR 55.0%
AGCGAGAAAGGACCTTGGGT+TGG - chr8_4:54356818-54356837 Msa1334050:three_prime_UTR 55.0%
GACTTGGGTGGTGTGGGAAT+AGG - chr8_4:54356840-54356859 Msa1334050:three_prime_UTR 55.0%
GGTGGTGTGGGAATAGGGTT+GGG - chr8_4:54356846-54356865 Msa1334050:three_prime_UTR 55.0%
!! ATAGGGTTGGGTGCTGGTCT+AGG - chr8_4:54356858-54356877 Msa1334050:three_prime_UTR 55.0%
!! TTGGGTGCTGGTCTAGGACT+TGG - chr8_4:54356864-54356883 Msa1334050:three_prime_UTR 55.0%
! GCTGGTCTAGGACTTGGACT+TGG - chr8_4:54356870-54356889 Msa1334050:three_prime_UTR 55.0%
! GGTCTAGGACTTGGACTTGG+TGG - chr8_4:54356873-54356892 Msa1334050:three_prime_UTR 55.0%
!! CTAGGACTTGGACTTGGTGG+TGG - chr8_4:54356876-54356895 Msa1334050:three_prime_UTR 55.0%
! GTCTGGTTCAGGGTCTGGTT+CGG - chr8_4:54356929-54356948 Msa1334050:three_prime_UTR 55.0%
! TCTGGTTCAGGGTCTGGTTC+GGG - chr8_4:54356930-54356949 Msa1334050:three_prime_UTR 55.0%
!! TCTGGTTCGGGTTCTGGTTC+TGG - chr8_4:54356942-54356961 Msa1334050:three_prime_UTR 55.0%
TCTGCTTCAGGATCGGGATC+AGG - chr8_4:54356966-54356985 Msa1334050:three_prime_UTR 55.0%
CTGCTTCAGGATCGGGATCA+GGG - chr8_4:54356967-54356986 Msa1334050:three_prime_UTR 55.0%
!! TCTGGAGCTGGTTCTGAAGC+CGG - chr8_4:54356993-54357012 Msa1334050:three_prime_UTR 55.0%
ACGAGATCCTGCATGAGAGC+CGG + chr8_4:54357015-54357034 None:intergenic 55.0%
TCTCATGCAGGATCTCGTGC+AGG - chr8_4:54357017-54357036 Msa1334050:exon 55.0%
CTCATGCAGGATCTCGTGCA+GGG - chr8_4:54357018-54357037 Msa1334050:exon 55.0%
CAGGTTCTCATGCTGGGTCA+GGG - chr8_4:54357084-54357103 Msa1334050:CDS 55.0%
! TCTGGAGCAGGTTCAGAAGC+CGG - chr8_4:54357110-54357129 Msa1334050:intron 55.0%
!! TCAGAAGCCGGTTCTGAAGC+CGG - chr8_4:54357122-54357141 Msa1334050:intron 55.0%
!! TCAGGGTCTGGTTCTGAAGC+TGG - chr8_4:54357170-54357189 Msa1334050:intron 55.0%
TCATATGCAGGGTCCCATGC+TGG - chr8_4:54357194-54357213 Msa1334050:CDS 55.0%
TCCCATGCTGGATCAGGAAG+TGG - chr8_4:54357206-54357225 Msa1334050:CDS 55.0%
AGAGCAGCGAGAAAGGACCT+TGG - chr8_4:54356813-54356832 Msa1334050:three_prime_UTR 55.0%
GAGCAGCGAGAAAGGACCTT+GGG - chr8_4:54356814-54356833 Msa1334050:three_prime_UTR 55.0%
AGCGAGAAAGGACCTTGGGT+TGG - chr8_4:54356818-54356837 Msa1334050:three_prime_UTR 55.0%
GACTTGGGTGGTGTGGGAAT+AGG - chr8_4:54356840-54356859 Msa1334050:three_prime_UTR 55.0%
GGTGGTGTGGGAATAGGGTT+GGG - chr8_4:54356846-54356865 Msa1334050:three_prime_UTR 55.0%
!! ATAGGGTTGGGTGCTGGTCT+AGG - chr8_4:54356858-54356877 Msa1334050:three_prime_UTR 55.0%
!! TTGGGTGCTGGTCTAGGACT+TGG - chr8_4:54356864-54356883 Msa1334050:three_prime_UTR 55.0%
! GCTGGTCTAGGACTTGGACT+TGG - chr8_4:54356870-54356889 Msa1334050:three_prime_UTR 55.0%
! GGTCTAGGACTTGGACTTGG+TGG - chr8_4:54356873-54356892 Msa1334050:three_prime_UTR 55.0%
!! CTAGGACTTGGACTTGGTGG+TGG - chr8_4:54356876-54356895 Msa1334050:three_prime_UTR 55.0%
! GTCTGGTTCAGGGTCTGGTT+CGG - chr8_4:54356929-54356948 Msa1334050:three_prime_UTR 55.0%
! TCTGGTTCAGGGTCTGGTTC+GGG - chr8_4:54356930-54356949 Msa1334050:three_prime_UTR 55.0%
!! TCTGGTTCGGGTTCTGGTTC+TGG - chr8_4:54356942-54356961 Msa1334050:three_prime_UTR 55.0%
TCTGCTTCAGGATCGGGATC+AGG - chr8_4:54356966-54356985 Msa1334050:three_prime_UTR 55.0%
CTGCTTCAGGATCGGGATCA+GGG - chr8_4:54356967-54356986 Msa1334050:three_prime_UTR 55.0%
!! TCTGGAGCTGGTTCTGAAGC+CGG - chr8_4:54356993-54357012 Msa1334050:three_prime_UTR 55.0%
ACGAGATCCTGCATGAGAGC+CGG + chr8_4:54357015-54357034 None:intergenic 55.0%
TCTCATGCAGGATCTCGTGC+AGG - chr8_4:54357017-54357036 Msa1334050:exon 55.0%
CTCATGCAGGATCTCGTGCA+GGG - chr8_4:54357018-54357037 Msa1334050:exon 55.0%
CAGGTTCTCATGCTGGGTCA+GGG - chr8_4:54357084-54357103 Msa1334050:CDS 55.0%
! TCTGGAGCAGGTTCAGAAGC+CGG - chr8_4:54357110-54357129 Msa1334050:intron 55.0%
!! TCAGAAGCCGGTTCTGAAGC+CGG - chr8_4:54357122-54357141 Msa1334050:intron 55.0%
!! TCAGGGTCTGGTTCTGAAGC+TGG - chr8_4:54357170-54357189 Msa1334050:intron 55.0%
TCATATGCAGGGTCCCATGC+TGG - chr8_4:54357194-54357213 Msa1334050:CDS 55.0%
TCCCATGCTGGATCAGGAAG+TGG - chr8_4:54357206-54357225 Msa1334050:CDS 55.0%
!! GACCTTGGGTTGGACTTGGG+TGG - chr8_4:54356828-54356847 Msa1334050:three_prime_UTR 60.0%
CACCACCCAAGTCCAACCCA+AGG + chr8_4:54356833-54356852 None:intergenic 60.0%
! TGGGTTGGACTTGGGTGGTG+TGG - chr8_4:54356833-54356852 Msa1334050:three_prime_UTR 60.0%
GGGTTGGACTTGGGTGGTGT+GGG - chr8_4:54356834-54356853 Msa1334050:three_prime_UTR 60.0%
GGGTGGTGTGGGAATAGGGT+TGG - chr8_4:54356845-54356864 Msa1334050:three_prime_UTR 60.0%
GTGGGAATAGGGTTGGGTGC+TGG - chr8_4:54356852-54356871 Msa1334050:three_prime_UTR 60.0%
!! GGACTTGGACTTGGTGGTGG+TGG - chr8_4:54356879-54356898 Msa1334050:three_prime_UTR 60.0%
!! CTTGGACTTGGTGGTGGTGG+TGG - chr8_4:54356882-54356901 Msa1334050:three_prime_UTR 60.0%
!! TCAGGGTCTGGTTCGGGTTC+TGG - chr8_4:54356936-54356955 Msa1334050:three_prime_UTR 60.0%
GGATCGGGATCAGGGTCTTC+TGG - chr8_4:54356975-54356994 Msa1334050:three_prime_UTR 60.0%
!! GGATCAGGGTCTTCTGGAGC+TGG - chr8_4:54356981-54357000 Msa1334050:three_prime_UTR 60.0%
! TCTGAAGCCGGCTCTCATGC+AGG - chr8_4:54357005-54357024 Msa1334050:three_prime_UTR 60.0%
CAGGATCTCGTGCAGGGTCA+GGG - chr8_4:54357024-54357043 Msa1334050:exon 60.0%
AGGATCTCGTGCAGGGTCAG+GGG - chr8_4:54357025-54357044 Msa1334050:exon 60.0%
GCAGGTTCTCATGCTGGGTC+AGG - chr8_4:54357083-54357102 Msa1334050:CDS 60.0%
CATGCTGGGTCAGGGTCTTC+TGG - chr8_4:54357092-54357111 Msa1334050:CDS 60.0%
CAGGGTCTCATGCTGGGTCA+GGG - chr8_4:54357153-54357172 Msa1334050:intron 60.0%
TCTCATGCTGGGTCAGGGTC+TGG - chr8_4:54357089-54357108 Msa1334050:CDS 60.0%
!! GACCTTGGGTTGGACTTGGG+TGG - chr8_4:54356828-54356847 Msa1334050:three_prime_UTR 60.0%
CACCACCCAAGTCCAACCCA+AGG + chr8_4:54356833-54356852 None:intergenic 60.0%
! TGGGTTGGACTTGGGTGGTG+TGG - chr8_4:54356833-54356852 Msa1334050:three_prime_UTR 60.0%
GGGTTGGACTTGGGTGGTGT+GGG - chr8_4:54356834-54356853 Msa1334050:three_prime_UTR 60.0%
GGGTGGTGTGGGAATAGGGT+TGG - chr8_4:54356845-54356864 Msa1334050:three_prime_UTR 60.0%
GTGGGAATAGGGTTGGGTGC+TGG - chr8_4:54356852-54356871 Msa1334050:three_prime_UTR 60.0%
!! GGACTTGGACTTGGTGGTGG+TGG - chr8_4:54356879-54356898 Msa1334050:three_prime_UTR 60.0%
!! CTTGGACTTGGTGGTGGTGG+TGG - chr8_4:54356882-54356901 Msa1334050:three_prime_UTR 60.0%
!! TCAGGGTCTGGTTCGGGTTC+TGG - chr8_4:54356936-54356955 Msa1334050:three_prime_UTR 60.0%
GGATCGGGATCAGGGTCTTC+TGG - chr8_4:54356975-54356994 Msa1334050:three_prime_UTR 60.0%
!! GGATCAGGGTCTTCTGGAGC+TGG - chr8_4:54356981-54357000 Msa1334050:three_prime_UTR 60.0%
! TCTGAAGCCGGCTCTCATGC+AGG - chr8_4:54357005-54357024 Msa1334050:three_prime_UTR 60.0%
CAGGATCTCGTGCAGGGTCA+GGG - chr8_4:54357024-54357043 Msa1334050:exon 60.0%
AGGATCTCGTGCAGGGTCAG+GGG - chr8_4:54357025-54357044 Msa1334050:exon 60.0%
GCAGGTTCTCATGCTGGGTC+AGG - chr8_4:54357083-54357102 Msa1334050:CDS 60.0%
CATGCTGGGTCAGGGTCTTC+TGG - chr8_4:54357092-54357111 Msa1334050:CDS 60.0%
CAGGGTCTCATGCTGGGTCA+GGG - chr8_4:54357153-54357172 Msa1334050:intron 60.0%
TCTCATGCTGGGTCAGGGTC+TGG - chr8_4:54357089-54357108 Msa1334050:CDS 60.0%
!! CTTGGTGGTGGTGGTGGTGC+TGG - chr8_4:54356888-54356907 Msa1334050:three_prime_UTR 65.0%
CCCTGAACCAGACCCTGCAC+CGG + chr8_4:54356922-54356941 None:intergenic 65.0%
! CCGGTGCAGGGTCTGGTTCA+GGG - chr8_4:54356919-54356938 Msa1334050:three_prime_UTR 65.0%
! GCAGGGTCTGGTTCAGGGTC+TGG - chr8_4:54356924-54356943 Msa1334050:three_prime_UTR 65.0%
GCAGGATCTCGTGCAGGGTC+AGG - chr8_4:54357023-54357042 Msa1334050:exon 65.0%
!! GGGTCAGGGTCTTCTGGAGC+AGG - chr8_4:54357098-54357117 Msa1334050:intron 65.0%
GCAGGGTCTCATGCTGGGTC+AGG - chr8_4:54357152-54357171 Msa1334050:intron 65.0%
GCAGGGTCCCATGCTGGATC+AGG - chr8_4:54357200-54357219 Msa1334050:CDS 65.0%
!! CTTGGTGGTGGTGGTGGTGC+TGG - chr8_4:54356888-54356907 Msa1334050:three_prime_UTR 65.0%
CCCTGAACCAGACCCTGCAC+CGG + chr8_4:54356922-54356941 None:intergenic 65.0%
! CCGGTGCAGGGTCTGGTTCA+GGG - chr8_4:54356919-54356938 Msa1334050:three_prime_UTR 65.0%
! GCAGGGTCTGGTTCAGGGTC+TGG - chr8_4:54356924-54356943 Msa1334050:three_prime_UTR 65.0%
GCAGGATCTCGTGCAGGGTC+AGG - chr8_4:54357023-54357042 Msa1334050:exon 65.0%
!! GGGTCAGGGTCTTCTGGAGC+AGG - chr8_4:54357098-54357117 Msa1334050:intron 65.0%
GCAGGGTCTCATGCTGGGTC+AGG - chr8_4:54357152-54357171 Msa1334050:intron 65.0%
GCAGGGTCCCATGCTGGATC+AGG - chr8_4:54357200-54357219 Msa1334050:CDS 65.0%
!! GGTGGTGGTGGTGCTGGCTC+TGG - chr8_4:54356894-54356913 Msa1334050:three_prime_UTR 70.0%
!! GGTGGTGCTGGCTCTGGTGC+CGG - chr8_4:54356900-54356919 Msa1334050:three_prime_UTR 70.0%
!! CTGGCTCTGGTGCCGGTGCA+GGG - chr8_4:54356907-54356926 Msa1334050:three_prime_UTR 70.0%
!! TCTGGTGCCGGTGCAGGGTC+TGG - chr8_4:54356912-54356931 Msa1334050:three_prime_UTR 70.0%
! GCCGGTGCAGGGTCTGGTTC+AGG - chr8_4:54356918-54356937 Msa1334050:three_prime_UTR 70.0%
TCTCGTGCAGGGTCAGGGGC+TGG - chr8_4:54357029-54357048 Msa1334050:exon 70.0%
CTCGTGCAGGGTCAGGGGCT+GGG - chr8_4:54357030-54357049 Msa1334050:exon 70.0%
!! GGTGGTGGTGGTGCTGGCTC+TGG - chr8_4:54356894-54356913 Msa1334050:three_prime_UTR 70.0%
!! GGTGGTGCTGGCTCTGGTGC+CGG - chr8_4:54356900-54356919 Msa1334050:three_prime_UTR 70.0%
!! CTGGCTCTGGTGCCGGTGCA+GGG - chr8_4:54356907-54356926 Msa1334050:three_prime_UTR 70.0%
!! TCTGGTGCCGGTGCAGGGTC+TGG - chr8_4:54356912-54356931 Msa1334050:three_prime_UTR 70.0%
! GCCGGTGCAGGGTCTGGTTC+AGG - chr8_4:54356918-54356937 Msa1334050:three_prime_UTR 70.0%
TCTCGTGCAGGGTCAGGGGC+TGG - chr8_4:54357029-54357048 Msa1334050:exon 70.0%
CTCGTGCAGGGTCAGGGGCT+GGG - chr8_4:54357030-54357049 Msa1334050:exon 70.0%
!! GCTGGCTCTGGTGCCGGTGC+AGG - chr8_4:54356906-54356925 Msa1334050:three_prime_UTR 75.0%
GCAGGGTCAGGGGCTGGGTC+AGG - chr8_4:54357035-54357054 Msa1334050:CDS 75.0%
!! GCTGGCTCTGGTGCCGGTGC+AGG - chr8_4:54356906-54356925 Msa1334050:three_prime_UTR 75.0%
GCAGGGTCAGGGGCTGGGTC+AGG - chr8_4:54357035-54357054 Msa1334050:CDS 75.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 54356744 54357525 54356744 ID=Msa1334050;Name=Msa1334050
chr8_4 mRNA 54356744 54357525 54356744 ID=Msa1334050-mRNA-1;Parent=Msa1334050;Name=Msa1334050-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0|0.5|0.33|1|0|0|3|290|136
chr8_4 exon 54356744 54357113 54356744 ID=Msa1334050-mRNA-1:exon:16904;Parent=Msa1334050-mRNA-1
chr8_4 exon 54357183 54357293 54357183 ID=Msa1334050-mRNA-1:exon:16903;Parent=Msa1334050-mRNA-1
chr8_4 exon 54357306 54357525 54357306 ID=Msa1334050-mRNA-1:exon:16902;Parent=Msa1334050-mRNA-1
chr8_4 CDS 54357306 54357525 54357306 ID=Msa1334050-mRNA-1:cds;Parent=Msa1334050-mRNA-1
chr8_4 CDS 54357183 54357293 54357183 ID=Msa1334050-mRNA-1:cds;Parent=Msa1334050-mRNA-1
chr8_4 CDS 54357034 54357113 54357034 ID=Msa1334050-mRNA-1:cds;Parent=Msa1334050-mRNA-1
chr8_4 three_prime_UTR 54356744 54357033 54356744 ID=Msa1334050-mRNA-1:three_prime_utr;Parent=Msa1334050-mRNA-1
Gene Sequence

>Msa1334050

ATGGCTTCAATGAAGATTTTAGTTGTTGTTTCTTTGCTTGTTGCAATTAATGCTATTGCATGTGAAAGTAGAGCAGCGAGAAAGGACCTTGGGTTGGACTTGGGTGGTGTGGGAATAGGGTTGGGTGCTGGTCTAGGACTTGGACTTGGTGGTGGTGGTGGTGCTGGCTCTGGTGCCGGTGCAGGGTCTGGTTCAGGGTCTGGTTCGGGTTCTGGTTCTGGATCGGGATCAGGGTCTTCTGGAGCTGGTTCTGAAGCCGGCTCTCATGCAGGATCTCGTGCAGGGTCAGGGGCTGGGTCAGGAGCAGATTCTGAAGCCGGTTCATATGCAGGGTCTCATGCTGGGTCAGGGTCTGGTTCTGAAGCTGGTTCATATGCAGGGTCCCATGCTGGATCAGGAAGTGGAAATTAA
Protein sequence

>Msa1334050

MASMKILVVVSLLVAINAIACESRAARKDLGLDLGGVGIGLGAGLGLGLGGGGGAGSGAGAGSGSGSGSGSGSGSGSGSSGAGSEAGSHAGSRAGSGAGSGADSEAGSYAGSHAGSGSGSEAGSYAGSHAGSGSGN*