Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1358650 | XP_003603835.1 | 95.951 | 247 | 8 | 2 | 1 | 247 | 1 | 245 | 3.02e-172 | 484 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1358650 | sp|Q9SGS5|Y1607_ARATH | 30.996 | 271 | 130 | 9 | 14 | 246 | 21 | 272 | 6.84e-29 | 113 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1358650 | G7J5W8 | 95.951 | 247 | 8 | 2 | 1 | 247 | 1 | 245 | 1.44e-172 | 484 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0051480 | Msa1358650 | 0.822926 | 1.823563e-53 | -8.615850e-47 |
| Msa0052270 | Msa1358650 | 0.818174 | 2.249034e-52 | -8.615850e-47 |
| Msa0083890 | Msa1358650 | 0.813880 | 2.045763e-51 | -8.615850e-47 |
| Msa1358650 | Msa1377050 | 0.838038 | 3.645664e-57 | -8.615850e-47 |
| Msa1358650 | Msa1453600 | 0.845923 | 2.999727e-59 | -8.615850e-47 |
| Msa0660650 | Msa1358650 | 0.802345 | 5.862552e-49 | -8.615850e-47 |
| Msa0146200 | Msa1358650 | 0.829952 | 3.858888e-55 | -8.615850e-47 |
| Msa1262540 | Msa1358650 | 0.801009 | 1.102143e-48 | -8.615850e-47 |
| Msa0402800 | Msa1358650 | 0.822553 | 2.227741e-53 | -8.615850e-47 |
| Msa0438070 | Msa1358650 | 0.820854 | 5.502935e-53 | -8.615850e-47 |
| Msa0481770 | Msa1358650 | 0.839835 | 1.249027e-57 | -8.615850e-47 |
| Msa1026080 | Msa1358650 | 0.806174 | 9.350945e-50 | -8.615850e-47 |
| Msa0497860 | Msa1358650 | 0.808244 | 3.406852e-50 | -8.615850e-47 |
| Msa0523080 | Msa1358650 | 0.801289 | 9.661304e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1358650 | MtrunA17_Chr3g0143431 | 95.951 | 247 | 8 | 2 | 1 | 247 | 1 | 245 | 2.78e-176 | 484 |
| Msa1358650 | MtrunA17_Chr1g0157191 | 50.775 | 258 | 96 | 10 | 2 | 246 | 4 | 243 | 3.61e-67 | 207 |
| Msa1358650 | MtrunA17_Chr5g0406131 | 32.749 | 171 | 87 | 4 | 1 | 167 | 1 | 147 | 5.49e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1358650 | AT1G76070.1 | 30.996 | 271 | 130 | 9 | 14 | 246 | 21 | 272 | 6.95e-30 | 113 |
| Msa1358650 | AT1G20310.1 | 29.104 | 268 | 135 | 11 | 1 | 243 | 1 | 238 | 2.02e-18 | 82.0 |
Find 48 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCTTCTTCCCTCAGATTT+TGG | 0.122137 | tig0016355:+37621 | None:intergenic |
| ATCAATTCCATTAATATCTT+TGG | 0.179497 | tig0016355:+37782 | None:intergenic |
| TCTAGCTTCATGTGGAATTA+TGG | 0.187247 | tig0016355:+37812 | None:intergenic |
| CTTGGTGGTGGTGGTGTTGC+TGG | 0.256841 | tig0016355:-37369 | Msa1358650:CDS |
| CCAAGTTTGATCTAATTTAC+TGG | 0.265351 | tig0016355:+37255 | None:intergenic |
| AGAAAGTCCAAAGATATTAA+TGG | 0.282367 | tig0016355:-37789 | Msa1358650:CDS |
| AGATCTTGTTCCTTAGCTTA+AGG | 0.361689 | tig0016355:+37976 | None:intergenic |
| GGTGTTGCTGGTTGTAGTCT+TGG | 0.383396 | tig0016355:-37357 | Msa1358650:CDS |
| AAAAGATGTTATTTCATGTC+GGG | 0.387650 | tig0016355:-37647 | Msa1358650:CDS |
| ATCCCTTTCTCTGCACCTCT+TGG | 0.403077 | tig0016355:-37387 | Msa1358650:CDS |
| TCCACCTAGGCCTCTACAAC+TGG | 0.412969 | tig0016355:-37280 | Msa1358650:CDS |
| CTTGATTTGTGATCCCTACC+TGG | 0.418438 | tig0016355:+37900 | None:intergenic |
| CCTTTCTCTGCACCTCTTGG+TGG | 0.430613 | tig0016355:-37384 | Msa1358650:CDS |
| TCTGCACCTCTTGGTGGTGG+TGG | 0.444151 | tig0016355:-37378 | Msa1358650:CDS |
| TGTGGAATTATGGATGCAAT+TGG | 0.444954 | tig0016355:+37822 | None:intergenic |
| GACTTTCTTCTAGCTTCATG+TGG | 0.470996 | tig0016355:+37804 | None:intergenic |
| GGATCCAGTTGTAGAGGCCT+AGG | 0.476355 | tig0016355:+37276 | None:intergenic |
| TTCTCTGCACCTCTTGGTGG+TGG | 0.477822 | tig0016355:-37381 | Msa1358650:CDS |
| TAGTAATTATCTATTTCCTC+TGG | 0.477991 | tig0016355:+37468 | None:intergenic |
| CACGTAAAGAAATTAATCTA+TGG | 0.479566 | tig0016355:-37320 | Msa1358650:CDS |
| CCACCAAGAGGTGCAGAGAA+AGG | 0.489526 | tig0016355:+37384 | None:intergenic |
| AACCAAAATCTGAGGGAAGA+AGG | 0.495894 | tig0016355:-37623 | Msa1358650:CDS |
| GACAAGATAACGCAACAAAG+TGG | 0.496463 | tig0016355:-37878 | Msa1358650:CDS |
| TGATCCCTACCTGGACTGAA+TGG | 0.498481 | tig0016355:+37909 | None:intergenic |
| CACCAAGAGGTGCAGAGAAA+GGG | 0.499821 | tig0016355:+37385 | None:intergenic |
| CAAAAGATGTTATTTCATGT+CGG | 0.505412 | tig0016355:-37648 | Msa1358650:CDS |
| AGATAAGAAGTATGCAATTG+AGG | 0.507400 | tig0016355:-37577 | Msa1358650:CDS |
| CATAGATTAATTTCTTTACG+TGG | 0.529657 | tig0016355:+37321 | None:intergenic |
| GGCAAGACCTCATGGTGTCA+AGG | 0.540397 | tig0016355:-37856 | Msa1358650:CDS |
| CAAAACCATCCATTCAGTCC+AGG | 0.540895 | tig0016355:-37918 | Msa1358650:CDS |
| TGATGATGATGAAGAAGAAG+AGG | 0.542344 | tig0016355:-37418 | Msa1358650:CDS |
| CCCTACCTGGACTGAATGGA+TGG | 0.542832 | tig0016355:+37913 | None:intergenic |
| AAATTAATCTATGGAAGAGA+AGG | 0.544809 | tig0016355:-37311 | Msa1358650:CDS |
| CCATCCATTCAGTCCAGGTA+GGG | 0.567822 | tig0016355:-37913 | Msa1358650:CDS |
| TAGAGGCCTAGGTGGAGCCA+TGG | 0.570574 | tig0016355:+37287 | None:intergenic |
| TTTACTGGATCCAGTTGTAG+AGG | 0.574544 | tig0016355:+37270 | None:intergenic |
| ACAAAGTGGGCAAGACCTCA+TGG | 0.574674 | tig0016355:-37864 | Msa1358650:CDS |
| GCAACACCACCACCACCAAG+AGG | 0.576743 | tig0016355:+37372 | None:intergenic |
| GCAAGACCTCATGGTGTCAA+GGG | 0.587142 | tig0016355:-37855 | Msa1358650:CDS |
| TGTCGGGAAACCAAAATCTG+AGG | 0.596722 | tig0016355:-37631 | Msa1358650:CDS |
| ACAAGATAACGCAACAAAGT+GGG | 0.602580 | tig0016355:-37877 | Msa1358650:CDS |
| GTCGGGAAACCAAAATCTGA+GGG | 0.603177 | tig0016355:-37630 | Msa1358650:CDS |
| TCCAGTTGTAGAGGCCTAGG+TGG | 0.615336 | tig0016355:+37279 | None:intergenic |
| GAAGGACCATGGCTCCACCT+AGG | 0.627085 | tig0016355:-37293 | Msa1358650:CDS |
| GAAGGCTCAGGTAGCACCAG+AGG | 0.628886 | tig0016355:-37484 | Msa1358650:CDS |
| GAAAATCCCTTGACACCATG+AGG | 0.634828 | tig0016355:+37849 | None:intergenic |
| ACCATCCATTCAGTCCAGGT+AGG | 0.635976 | tig0016355:-37914 | Msa1358650:CDS |
| TCTATGGAAGAGAAGGACCA+TGG | 0.674583 | tig0016355:-37304 | Msa1358650:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGTTTATAGTGATAGTTTT+TGG | + | tig0016355:37314-37333 | None:intergenic | 20.0% |
| !! | ATCAATTCCATTAATATCTT+TGG | + | tig0016355:37474-37493 | None:intergenic | 20.0% |
| ! | AGAAAGTCCAAAGATATTAA+TGG | - | tig0016355:37464-37483 | Msa1358650:CDS | 25.0% |
| !!! | CAAAAGATGTTATTTCATGT+CGG | - | tig0016355:37605-37624 | Msa1358650:CDS | 25.0% |
| !!! | AAAAGATGTTATTTCATGTC+GGG | - | tig0016355:37606-37625 | Msa1358650:CDS | 25.0% |
| !!! | ACACTTTTGCTAATTTTGAT+TGG | - | tig0016355:37747-37766 | Msa1358650:CDS | 25.0% |
| !!! | TTTTGCTAATTTTGATTGGA+AGG | - | tig0016355:37751-37770 | Msa1358650:CDS | 25.0% |
| ! | TAGTAATTATCTATTTCCTC+TGG | + | tig0016355:37788-37807 | None:intergenic | 25.0% |
| ! | CATAGATTAATTTCTTTACG+TGG | + | tig0016355:37935-37954 | None:intergenic | 25.0% |
| ! | CACGTAAAGAAATTAATCTA+TGG | - | tig0016355:37933-37952 | Msa1358650:CDS | 25.0% |
| ! | AAATTAATCTATGGAAGAGA+AGG | - | tig0016355:37942-37961 | Msa1358650:CDS | 25.0% |
| ! | AGATAAGAAGTATGCAATTG+AGG | - | tig0016355:37676-37695 | Msa1358650:CDS | 30.0% |
| TGGAGAAAAACCTTAAGCTA+AGG | - | tig0016355:37267-37286 | Msa1358650:CDS | 35.0% | |
| AGATCTTGTTCCTTAGCTTA+AGG | + | tig0016355:37280-37299 | None:intergenic | 35.0% | |
| ACAAGATAACGCAACAAAGT+GGG | - | tig0016355:37376-37395 | Msa1358650:CDS | 35.0% | |
| TGTGGAATTATGGATGCAAT+TGG | + | tig0016355:37434-37453 | None:intergenic | 35.0% | |
| ! | TCTAGCTTCATGTGGAATTA+TGG | + | tig0016355:37444-37463 | None:intergenic | 35.0% |
| !!! | CACGATATTTTTGGTGAAGT+GGG | + | tig0016355:37506-37525 | None:intergenic | 35.0% |
| TTCACCAAAAATATCGTGCA+TGG | - | tig0016355:37508-37527 | Msa1358650:CDS | 35.0% | |
| TCACCAAAAATATCGTGCAT+GGG | - | tig0016355:37509-37528 | Msa1358650:CDS | 35.0% | |
| ! | CAAATGAAACGTTTTGCAAG+TGG | - | tig0016355:37719-37738 | Msa1358650:CDS | 35.0% |
| !!! | TAATTTTGATTGGAAGGCTC+AGG | - | tig0016355:37757-37776 | Msa1358650:CDS | 35.0% |
| ! | TGATGATGATGAAGAAGAAG+AGG | - | tig0016355:37835-37854 | Msa1358650:CDS | 35.0% |
| GACAAGATAACGCAACAAAG+TGG | - | tig0016355:37375-37394 | Msa1358650:CDS | 40.0% | |
| !! | GACTTTCTTCTAGCTTCATG+TGG | + | tig0016355:37452-37471 | None:intergenic | 40.0% |
| !!! | GCACGATATTTTTGGTGAAG+TGG | + | tig0016355:37507-37526 | None:intergenic | 40.0% |
| ! | TGTCCCATGCACGATATTTT+TGG | + | tig0016355:37515-37534 | None:intergenic | 40.0% |
| AACCAAAATCTGAGGGAAGA+AGG | - | tig0016355:37630-37649 | Msa1358650:CDS | 40.0% | |
| ! | TTCCTTCTTCCCTCAGATTT+TGG | + | tig0016355:37635-37654 | None:intergenic | 40.0% |
| TTTACTGGATCCAGTTGTAG+AGG | + | tig0016355:37986-38005 | None:intergenic | 40.0% | |
| CAAAACCATCCATTCAGTCC+AGG | - | tig0016355:37335-37354 | Msa1358650:CDS | 45.0% | |
| !! | CTTGATTTGTGATCCCTACC+TGG | + | tig0016355:37356-37375 | None:intergenic | 45.0% |
| GAAAATCCCTTGACACCATG+AGG | + | tig0016355:37407-37426 | None:intergenic | 45.0% | |
| !! | CATGGTGTCAAGGGATTTTC+AGG | - | tig0016355:37407-37426 | Msa1358650:CDS | 45.0% |
| TGTCGGGAAACCAAAATCTG+AGG | - | tig0016355:37622-37641 | Msa1358650:CDS | 45.0% | |
| GTCGGGAAACCAAAATCTGA+GGG | - | tig0016355:37623-37642 | Msa1358650:CDS | 45.0% | |
| TCTATGGAAGAGAAGGACCA+TGG | - | tig0016355:37949-37968 | Msa1358650:CDS | 45.0% | |
| ACCATCCATTCAGTCCAGGT+AGG | - | tig0016355:37339-37358 | Msa1358650:CDS | 50.0% | |
| CCATCCATTCAGTCCAGGTA+GGG | - | tig0016355:37340-37359 | Msa1358650:CDS | 50.0% | |
| TGATCCCTACCTGGACTGAA+TGG | + | tig0016355:37347-37366 | None:intergenic | 50.0% | |
| ACAAAGTGGGCAAGACCTCA+TGG | - | tig0016355:37389-37408 | Msa1358650:CDS | 50.0% | |
| ! | GCAAGACCTCATGGTGTCAA+GGG | - | tig0016355:37398-37417 | Msa1358650:CDS | 50.0% |
| ATCCCTTTCTCTGCACCTCT+TGG | - | tig0016355:37866-37885 | Msa1358650:CDS | 50.0% | |
| ! | CACCAAGAGGTGCAGAGAAA+GGG | + | tig0016355:37871-37890 | None:intergenic | 50.0% |
| !! | GGTGTTGCTGGTTGTAGTCT+TGG | - | tig0016355:37896-37915 | Msa1358650:CDS | 50.0% |
| CCCTACCTGGACTGAATGGA+TGG | + | tig0016355:37343-37362 | None:intergenic | 55.0% | |
| ! | GGCAAGACCTCATGGTGTCA+AGG | - | tig0016355:37397-37416 | Msa1358650:CDS | 55.0% |
| ! | CCACCAAGAGGTGCAGAGAA+AGG | + | tig0016355:37872-37891 | None:intergenic | 55.0% |
| CCTTTCTCTGCACCTCTTGG+TGG | - | tig0016355:37869-37888 | Msa1358650:CDS | 55.0% | |
| TTCTCTGCACCTCTTGGTGG+TGG | - | tig0016355:37872-37891 | Msa1358650:CDS | 55.0% | |
| TCCACCTAGGCCTCTACAAC+TGG | - | tig0016355:37973-37992 | Msa1358650:CDS | 55.0% | |
| TCCAGTTGTAGAGGCCTAGG+TGG | + | tig0016355:37977-37996 | None:intergenic | 55.0% | |
| GGATCCAGTTGTAGAGGCCT+AGG | + | tig0016355:37980-37999 | None:intergenic | 55.0% | |
| GAAGGCTCAGGTAGCACCAG+AGG | - | tig0016355:37769-37788 | Msa1358650:CDS | 60.0% | |
| !! | TCTGCACCTCTTGGTGGTGG+TGG | - | tig0016355:37875-37894 | Msa1358650:CDS | 60.0% |
| GCAACACCACCACCACCAAG+AGG | + | tig0016355:37884-37903 | None:intergenic | 60.0% | |
| !! | CTTGGTGGTGGTGGTGTTGC+TGG | - | tig0016355:37884-37903 | Msa1358650:CDS | 60.0% |
| GAAGGACCATGGCTCCACCT+AGG | - | tig0016355:37960-37979 | Msa1358650:CDS | 60.0% | |
| TAGAGGCCTAGGTGGAGCCA+TGG | + | tig0016355:37969-37988 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0016355 | gene | 37266 | 38009 | 37266 | ID=Msa1358650;Name=Msa1358650 |
| tig0016355 | mRNA | 37266 | 38009 | 37266 | ID=Msa1358650-mRNA-1;Parent=Msa1358650;Name=Msa1358650-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|247 |
| tig0016355 | exon | 37266 | 38009 | 37266 | ID=Msa1358650-mRNA-1:exon:10947;Parent=Msa1358650-mRNA-1 |
| tig0016355 | CDS | 37266 | 38009 | 37266 | ID=Msa1358650-mRNA-1:cds;Parent=Msa1358650-mRNA-1 |
| Gene Sequence |
| Protein sequence |
