Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1363450 | XP_003592837.1 | 86.719 | 128 | 3 | 5 | 1 | 126 | 1 | 116 | 7.38e-66 | 205 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1363450 | G7IB02 | 86.719 | 128 | 3 | 5 | 1 | 126 | 1 | 116 | 3.52e-66 | 205 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0057300 | Msa1363450 | 0.806019 | 1.008103e-49 | -8.615850e-47 |
| Msa1361770 | Msa1363450 | 0.822250 | 2.618807e-53 | -8.615850e-47 |
| Msa1363450 | Msa1363590 | 0.896749 | 2.734726e-76 | -8.615850e-47 |
| Msa1363450 | Msa1447150 | 0.831457 | 1.651419e-55 | -8.615850e-47 |
| Msa1363450 | Msa1459180 | 0.807019 | 6.200835e-50 | -8.615850e-47 |
| Msa0829190 | Msa1363450 | 0.804797 | 1.818140e-49 | -8.615850e-47 |
| Msa0222920 | Msa1363450 | 0.807658 | 4.538771e-50 | -8.615850e-47 |
| Msa1265070 | Msa1363450 | 0.812259 | 4.638675e-51 | -8.615850e-47 |
| Msa1337730 | Msa1363450 | 0.816735 | 4.744022e-52 | -8.615850e-47 |
| Msa0432480 | Msa1363450 | 0.810729 | 9.973742e-51 | -8.615850e-47 |
| Msa0982240 | Msa1363450 | 0.830716 | 2.510285e-55 | -8.615850e-47 |
| Msa0348180 | Msa1363450 | 0.806438 | 8.227443e-50 | -8.615850e-47 |
| Msa1170800 | Msa1363450 | 0.818098 | 2.340324e-52 | -8.615850e-47 |
| Msa1221900 | Msa1363450 | 0.812265 | 4.624018e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1363450 | MtrunA17_Chr1g0213571 | 86.719 | 128 | 3 | 5 | 1 | 126 | 1 | 116 | 6.78e-70 | 205 |
| Msa1363450 | MtrunA17_Chr1g0190591 | 43.519 | 108 | 36 | 2 | 40 | 122 | 75 | 182 | 1.26e-22 | 87.8 |
| Msa1363450 | MtrunA17_Chr7g0274251 | 40.385 | 104 | 34 | 2 | 47 | 122 | 79 | 182 | 6.32e-20 | 80.5 |
| Msa1363450 | MtrunA17_Chr5g0402271 | 39.655 | 116 | 35 | 5 | 41 | 123 | 57 | 170 | 3.63e-15 | 67.8 |
| Msa1363450 | MtrunA17_Chr7g0270921 | 50.704 | 71 | 31 | 2 | 49 | 119 | 214 | 280 | 9.58e-15 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1363450 | AT2G31560.2 | 61.594 | 138 | 30 | 1 | 12 | 126 | 65 | 202 | 3.02e-53 | 166 |
| Msa1363450 | AT2G31560.1 | 61.594 | 138 | 30 | 1 | 12 | 126 | 65 | 202 | 3.02e-53 | 166 |
| Msa1363450 | AT2G31560.3 | 61.594 | 138 | 30 | 1 | 12 | 126 | 65 | 202 | 3.02e-53 | 166 |
| Msa1363450 | AT1G05870.4 | 60.403 | 149 | 26 | 4 | 10 | 126 | 42 | 189 | 2.06e-52 | 164 |
| Msa1363450 | AT1G05870.12 | 60.403 | 149 | 26 | 4 | 10 | 126 | 42 | 189 | 2.06e-52 | 164 |
| Msa1363450 | AT1G05870.8 | 60.403 | 149 | 26 | 4 | 10 | 126 | 42 | 189 | 2.06e-52 | 164 |
| Msa1363450 | AT1G05870.1 | 60.403 | 149 | 26 | 4 | 10 | 126 | 42 | 189 | 2.06e-52 | 164 |
| Msa1363450 | AT1G05870.7 | 60.403 | 149 | 26 | 4 | 10 | 126 | 42 | 189 | 2.06e-52 | 164 |
| Msa1363450 | AT1G05870.2 | 60.403 | 149 | 26 | 4 | 10 | 126 | 42 | 189 | 2.06e-52 | 164 |
| Msa1363450 | AT1G05870.10 | 60.403 | 149 | 26 | 4 | 10 | 126 | 42 | 189 | 2.06e-52 | 164 |
| Msa1363450 | AT1G05870.5 | 60.403 | 149 | 26 | 4 | 10 | 126 | 42 | 189 | 2.06e-52 | 164 |
| Msa1363450 | AT1G05870.9 | 60.403 | 149 | 26 | 4 | 10 | 126 | 42 | 189 | 2.06e-52 | 164 |
| Msa1363450 | AT1G05870.3 | 60.403 | 149 | 26 | 4 | 10 | 126 | 42 | 189 | 2.06e-52 | 164 |
| Msa1363450 | AT1G05870.6 | 60.403 | 149 | 26 | 4 | 10 | 126 | 42 | 189 | 2.06e-52 | 164 |
| Msa1363450 | AT1G05870.11 | 60.403 | 149 | 26 | 4 | 10 | 126 | 42 | 189 | 2.06e-52 | 164 |
| Msa1363450 | AT2G43340.1 | 57.718 | 149 | 35 | 3 | 3 | 126 | 44 | 189 | 2.69e-47 | 150 |
| Msa1363450 | AT3G22690.1 | 61.728 | 81 | 23 | 1 | 45 | 117 | 843 | 923 | 1.86e-24 | 97.4 |
| Msa1363450 | AT3G04700.1 | 39.316 | 117 | 37 | 2 | 37 | 122 | 74 | 187 | 3.64e-19 | 79.0 |
| Msa1363450 | AT5G28690.1 | 37.903 | 124 | 44 | 3 | 29 | 122 | 68 | 188 | 8.52e-19 | 78.2 |
| Msa1363450 | AT5G28690.3 | 37.903 | 124 | 44 | 3 | 29 | 122 | 68 | 188 | 8.52e-19 | 78.2 |
| Msa1363450 | AT5G28690.2 | 37.903 | 124 | 44 | 3 | 29 | 122 | 68 | 188 | 8.52e-19 | 78.2 |
| Msa1363450 | AT1G08790.1 | 40.741 | 108 | 34 | 3 | 45 | 122 | 78 | 185 | 1.10e-18 | 77.8 |
| Msa1363450 | AT3G50350.2 | 42.056 | 107 | 43 | 2 | 35 | 122 | 59 | 165 | 1.71e-18 | 77.0 |
| Msa1363450 | AT3G04710.3 | 38.462 | 117 | 38 | 2 | 37 | 122 | 74 | 187 | 2.60e-18 | 80.1 |
| Msa1363450 | AT4G33985.1 | 45.370 | 108 | 39 | 2 | 39 | 126 | 43 | 150 | 3.17e-18 | 75.9 |
| Msa1363450 | AT2G15590.2 | 38.318 | 107 | 47 | 3 | 39 | 126 | 40 | 146 | 7.75e-13 | 61.6 |
| Msa1363450 | AT3G50350.1 | 51.613 | 62 | 28 | 1 | 35 | 94 | 63 | 124 | 1.61e-12 | 61.6 |
| Msa1363450 | AT3G62070.1 | 46.667 | 75 | 30 | 3 | 50 | 119 | 150 | 219 | 3.07e-12 | 61.6 |
| Msa1363450 | AT2G46940.1 | 47.143 | 70 | 29 | 3 | 50 | 119 | 185 | 246 | 1.28e-11 | 60.1 |
| Msa1363450 | AT4G01670.1 | 49.296 | 71 | 30 | 2 | 49 | 119 | 172 | 236 | 8.55e-11 | 57.8 |
Find 32 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATGTTTGGATCTTGGTTT+TGG | 0.184629 | tig0018043:+6589 | Msa1363450:CDS |
| TTAAAGCAAGATTGAAATAT+TGG | 0.250359 | tig0018043:+6746 | Msa1363450:CDS |
| AATCTTGCTTTAACATCTTC+AGG | 0.331635 | tig0018043:-6736 | None:intergenic |
| TTCCAATTGGCAAATGAATC+AGG | 0.369053 | tig0018043:-6691 | None:intergenic |
| CTCCTGATTCATTTGCCAAT+TGG | 0.369305 | tig0018043:+6689 | Msa1363450:CDS |
| TGATGAGTTGAAGGGATGTT+TGG | 0.374718 | tig0018043:+6576 | Msa1363450:CDS |
| TAAAGCAAGATTGAAATATT+GGG | 0.375422 | tig0018043:+6747 | Msa1363450:CDS |
| TTGAAGGGATGTTTGGATCT+TGG | 0.389102 | tig0018043:+6583 | Msa1363450:CDS |
| TAGTTAGCTGCAGAGTTTAA+CGG | 0.390779 | tig0018043:-6788 | None:intergenic |
| GGGAGGGTGTTACATAGTTC+AGG | 0.412792 | tig0018043:-6631 | None:intergenic |
| ATGGAAGGTGGAGGTGTTGT+TGG | 0.413737 | tig0018043:+6427 | Msa1363450:CDS |
| CATCAAGATCCTCATCTGTT+AGG | 0.424926 | tig0018043:-6558 | None:intergenic |
| TGGACTTGAGATCTTCCAAT+TGG | 0.470698 | tig0018043:-6704 | None:intergenic |
| GGAGGTGTTGTTGGAAGAAG+TGG | 0.473169 | tig0018043:+6436 | Msa1363450:CDS |
| TGAGGATCTTGATGAGTTGA+AGG | 0.481670 | tig0018043:+6567 | Msa1363450:CDS |
| TAACAAAGGAGTAGTGTTGT+TGG | 0.483852 | tig0018043:+6489 | Msa1363450:CDS |
| GAATAACACAACTCTAGAGC+TGG | 0.494397 | tig0018043:-6652 | None:intergenic |
| ATAATGAGGAGGATCTTTGT+AGG | 0.495986 | tig0018043:+6527 | Msa1363450:CDS |
| AAGGAGTAGTGTTGTTGGAA+GGG | 0.511274 | tig0018043:+6494 | Msa1363450:CDS |
| AAAGGAGTAGTGTTGTTGGA+AGG | 0.535317 | tig0018043:+6493 | Msa1363450:CDS |
| ACATCTTCAGGATGATCACC+TGG | 0.572260 | tig0018043:-6724 | None:intergenic |
| AGGGTATGTTGATGATAATG+AGG | 0.584846 | tig0018043:+6513 | Msa1363450:CDS |
| AATAACACAACTCTAGAGCT+GGG | 0.590951 | tig0018043:-6651 | None:intergenic |
| AATTGGAAGATCTCAAGTCC+AGG | 0.596552 | tig0018043:+6706 | Msa1363450:CDS |
| GTTGTTGGAAGAAGTGGAAG+TGG | 0.596897 | tig0018043:+6442 | Msa1363450:CDS |
| ACACAACTCTAGAGCTGGGA+GGG | 0.600482 | tig0018043:-6647 | None:intergenic |
| GACGAAAAGCCTAACAGATG+AGG | 0.616832 | tig0018043:+6549 | Msa1363450:CDS |
| TTGTTGGAAGAAGTGGAAGT+GGG | 0.619030 | tig0018043:+6443 | Msa1363450:CDS |
| AGTAAGAATAACAATAACAA+AGG | 0.625105 | tig0018043:+6475 | Msa1363450:CDS |
| GAGGATCTTGATGAGTTGAA+GGG | 0.630888 | tig0018043:+6568 | Msa1363450:CDS |
| GTATGTTGATGATAATGAGG+AGG | 0.675262 | tig0018043:+6516 | Msa1363450:CDS |
| AACACAACTCTAGAGCTGGG+AGG | 0.715725 | tig0018043:-6648 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTAAGAATAACAATAACAA+AGG | + | tig0018043:6475-6494 | Msa1363450:CDS | 20.0% |
| !! | TTAAAGCAAGATTGAAATAT+TGG | + | tig0018043:6746-6765 | Msa1363450:CDS | 20.0% |
| !! | TAAAGCAAGATTGAAATATT+GGG | + | tig0018043:6747-6766 | Msa1363450:CDS | 20.0% |
| AATCTTGCTTTAACATCTTC+AGG | - | tig0018043:6739-6758 | None:intergenic | 30.0% | |
| TAACAAAGGAGTAGTGTTGT+TGG | + | tig0018043:6489-6508 | Msa1363450:CDS | 35.0% | |
| !! | AGGGTATGTTGATGATAATG+AGG | + | tig0018043:6513-6532 | Msa1363450:CDS | 35.0% |
| !! | GTATGTTGATGATAATGAGG+AGG | + | tig0018043:6516-6535 | Msa1363450:CDS | 35.0% |
| ATAATGAGGAGGATCTTTGT+AGG | + | tig0018043:6527-6546 | Msa1363450:CDS | 35.0% | |
| !! | GATGTTTGGATCTTGGTTTT+GGG | + | tig0018043:6590-6609 | Msa1363450:CDS | 35.0% |
| !! | AATAACACAACTCTAGAGCT+GGG | - | tig0018043:6654-6673 | None:intergenic | 35.0% |
| TTCCAATTGGCAAATGAATC+AGG | - | tig0018043:6694-6713 | None:intergenic | 35.0% | |
| TTGTTGGAAGAAGTGGAAGT+GGG | + | tig0018043:6443-6462 | Msa1363450:CDS | 40.0% | |
| ! | AAAGGAGTAGTGTTGTTGGA+AGG | + | tig0018043:6493-6512 | Msa1363450:CDS | 40.0% |
| ! | AAGGAGTAGTGTTGTTGGAA+GGG | + | tig0018043:6494-6513 | Msa1363450:CDS | 40.0% |
| CATCAAGATCCTCATCTGTT+AGG | - | tig0018043:6561-6580 | None:intergenic | 40.0% | |
| ! | TGAGGATCTTGATGAGTTGA+AGG | + | tig0018043:6567-6586 | Msa1363450:CDS | 40.0% |
| ! | GAGGATCTTGATGAGTTGAA+GGG | + | tig0018043:6568-6587 | Msa1363450:CDS | 40.0% |
| ! | TGATGAGTTGAAGGGATGTT+TGG | + | tig0018043:6576-6595 | Msa1363450:CDS | 40.0% |
| TTGAAGGGATGTTTGGATCT+TGG | + | tig0018043:6583-6602 | Msa1363450:CDS | 40.0% | |
| ! | GGATGTTTGGATCTTGGTTT+TGG | + | tig0018043:6589-6608 | Msa1363450:CDS | 40.0% |
| !! | GAATAACACAACTCTAGAGC+TGG | - | tig0018043:6655-6674 | None:intergenic | 40.0% |
| CTCCTGATTCATTTGCCAAT+TGG | + | tig0018043:6689-6708 | Msa1363450:CDS | 40.0% | |
| TGGACTTGAGATCTTCCAAT+TGG | - | tig0018043:6707-6726 | None:intergenic | 40.0% | |
| AATTGGAAGATCTCAAGTCC+AGG | + | tig0018043:6706-6725 | Msa1363450:CDS | 40.0% | |
| GTTGTTGGAAGAAGTGGAAG+TGG | + | tig0018043:6442-6461 | Msa1363450:CDS | 45.0% | |
| GACGAAAAGCCTAACAGATG+AGG | + | tig0018043:6549-6568 | Msa1363450:CDS | 45.0% | |
| ACATCTTCAGGATGATCACC+TGG | - | tig0018043:6727-6746 | None:intergenic | 45.0% | |
| !! | ATGGAAGGTGGAGGTGTTGT+TGG | + | tig0018043:6427-6446 | Msa1363450:CDS | 50.0% |
| !! | GGAGGTGTTGTTGGAAGAAG+TGG | + | tig0018043:6436-6455 | Msa1363450:CDS | 50.0% |
| !! | GGGAGGGTGTTACATAGTTC+AGG | - | tig0018043:6634-6653 | None:intergenic | 50.0% |
| !! | ACACAACTCTAGAGCTGGGA+GGG | - | tig0018043:6650-6669 | None:intergenic | 50.0% |
| !! | AACACAACTCTAGAGCTGGG+AGG | - | tig0018043:6651-6670 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0018043 | gene | 6427 | 6807 | 6427 | ID=Msa1363450;Name=Msa1363450 |
| tig0018043 | mRNA | 6427 | 6807 | 6427 | ID=Msa1363450-mRNA-1;Parent=Msa1363450;Name=Msa1363450-mRNA-1;_AED=0.00;_eAED=0.06;_QI=0|-1|0|1|-1|1|1|0|126 |
| tig0018043 | exon | 6427 | 6807 | 6427 | ID=Msa1363450-mRNA-1:exon:13558;Parent=Msa1363450-mRNA-1 |
| tig0018043 | CDS | 6427 | 6807 | 6427 | ID=Msa1363450-mRNA-1:cds;Parent=Msa1363450-mRNA-1 |
| Gene Sequence |
| Protein sequence |
