Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1363590 | XP_003592837.1 | 85.385 | 130 | 3 | 5 | 1 | 128 | 1 | 116 | 1.53e-65 | 204 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1363590 | G7IB02 | 85.385 | 130 | 3 | 5 | 1 | 128 | 1 | 116 | 7.30e-66 | 204 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0008290 | Msa1363590 | 0.818513 | 1.885095e-52 | -8.615850e-47 |
| Msa0008340 | Msa1363590 | 0.804556 | 2.041298e-49 | -8.615850e-47 |
| Msa0022750 | Msa1363590 | -0.820222 | 7.687393e-53 | -8.615850e-47 |
| Msa0046100 | Msa1363590 | 0.825746 | 3.962206e-54 | -8.615850e-47 |
| Msa0048000 | Msa1363590 | 0.800263 | 1.564640e-48 | -8.615850e-47 |
| Msa0057300 | Msa1363590 | 0.814645 | 1.386151e-51 | -8.615850e-47 |
| Msa0094010 | Msa1363590 | 0.808359 | 3.220336e-50 | -8.615850e-47 |
| Msa0097840 | Msa1363590 | 0.802163 | 6.390548e-49 | -8.615850e-47 |
| Msa0105420 | Msa1363590 | 0.835368 | 1.747858e-56 | -8.615850e-47 |
| Msa1346370 | Msa1363590 | 0.802331 | 5.903315e-49 | -8.615850e-47 |
| Msa1361770 | Msa1363590 | 0.830313 | 3.149953e-55 | -8.615850e-47 |
| Msa1363450 | Msa1363590 | 0.896749 | 2.734726e-76 | -8.615850e-47 |
| Msa1363590 | Msa1370650 | 0.800990 | 1.111956e-48 | -8.615850e-47 |
| Msa1363590 | Msa1379850 | 0.817980 | 2.488185e-52 | -8.615850e-47 |
| Msa1363590 | Msa1390770 | 0.808507 | 2.993336e-50 | -8.615850e-47 |
| Msa1363590 | Msa1404540 | 0.806352 | 8.575605e-50 | -8.615850e-47 |
| Msa1363590 | Msa1431060 | 0.801387 | 9.221334e-49 | -8.615850e-47 |
| Msa1363590 | Msa1447150 | 0.850201 | 1.978962e-60 | -8.615850e-47 |
| Msa0691050 | Msa1363590 | 0.802920 | 4.461458e-49 | -8.615850e-47 |
| Msa0697400 | Msa1363590 | 0.809495 | 1.839990e-50 | -8.615850e-47 |
| Msa0724000 | Msa1363590 | 0.800165 | 1.637846e-48 | -8.615850e-47 |
| Msa0762210 | Msa1363590 | 0.804814 | 1.803324e-49 | -8.615850e-47 |
| Msa0798070 | Msa1363590 | 0.802033 | 6.796342e-49 | -8.615850e-47 |
| Msa0829190 | Msa1363590 | 0.807508 | 4.885223e-50 | -8.615850e-47 |
| Msa0222920 | Msa1363590 | 0.821510 | 3.884949e-53 | -8.615850e-47 |
| Msa1236100 | Msa1363590 | 0.812075 | 5.089020e-51 | -8.615850e-47 |
| Msa1239530 | Msa1363590 | 0.800869 | 1.177378e-48 | -8.615850e-47 |
| Msa1243010 | Msa1363590 | 0.810698 | 1.012984e-50 | -8.615850e-47 |
| Msa1255190 | Msa1363590 | 0.804671 | 1.931618e-49 | -8.615850e-47 |
| Msa1265070 | Msa1363590 | 0.831049 | 2.080436e-55 | -8.615850e-47 |
| Msa1295630 | Msa1363590 | 0.817703 | 2.873695e-52 | -8.615850e-47 |
| Msa1318120 | Msa1363590 | 0.804147 | 2.483773e-49 | -8.615850e-47 |
| Msa1337730 | Msa1363590 | 0.840319 | 9.339488e-58 | -8.615850e-47 |
| Msa0432480 | Msa1363590 | 0.813194 | 2.895401e-51 | -8.615850e-47 |
| Msa0436370 | Msa1363590 | 0.816937 | 4.274819e-52 | -8.615850e-47 |
| Msa0467930 | Msa1363590 | 0.807289 | 5.436972e-50 | -8.615850e-47 |
| Msa0982240 | Msa1363590 | 0.850045 | 2.187798e-60 | -8.615850e-47 |
| Msa0993530 | Msa1363590 | 0.814511 | 1.484388e-51 | -8.615850e-47 |
| Msa0264350 | Msa1363590 | 0.831659 | 1.472964e-55 | -8.615850e-47 |
| Msa0312990 | Msa1363590 | 0.824850 | 6.455326e-54 | -8.615850e-47 |
| Msa0343420 | Msa1363590 | 0.800530 | 1.380417e-48 | -8.615850e-47 |
| Msa0348180 | Msa1363590 | 0.814424 | 1.551247e-51 | -8.615850e-47 |
| Msa1127770 | Msa1363590 | 0.808319 | 3.283038e-50 | -8.615850e-47 |
| Msa1170800 | Msa1363590 | 0.840742 | 7.237391e-58 | -8.615850e-47 |
| Msa1179970 | Msa1363590 | 0.800190 | 1.618636e-48 | -8.615850e-47 |
| Msa1188910 | Msa1363590 | 0.814797 | 1.283177e-51 | -8.615850e-47 |
| Msa1221900 | Msa1363590 | 0.800493 | 1.404775e-48 | -8.615850e-47 |
| Msa0489030 | Msa1363590 | 0.809534 | 1.804585e-50 | -8.615850e-47 |
| Msa0489100 | Msa1363590 | 0.835342 | 1.774364e-56 | -8.615850e-47 |
| Msa0516370 | Msa1363590 | 0.802221 | 6.218187e-49 | -8.615850e-47 |
| Msa0544830 | Msa1363590 | 0.802492 | 5.468241e-49 | -8.615850e-47 |
| Msa0572420 | Msa1363590 | 0.808052 | 3.742063e-50 | -8.615850e-47 |
| Msa0893810 | Msa1363590 | 0.808287 | 3.335444e-50 | -8.615850e-47 |
| Msa0896220 | Msa1363590 | 0.828948 | 6.768570e-55 | -8.615850e-47 |
| Msa0908050 | Msa1363590 | 0.803805 | 2.925909e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1363590 | MtrunA17_Chr1g0213571 | 85.385 | 130 | 3 | 5 | 1 | 128 | 1 | 116 | 1.41e-69 | 204 |
| Msa1363590 | MtrunA17_Chr1g0190591 | 43.519 | 108 | 36 | 2 | 42 | 124 | 75 | 182 | 1.13e-22 | 87.8 |
| Msa1363590 | MtrunA17_Chr7g0274251 | 40.385 | 104 | 34 | 2 | 49 | 124 | 79 | 182 | 6.13e-20 | 80.9 |
| Msa1363590 | MtrunA17_Chr5g0402271 | 39.655 | 116 | 35 | 5 | 43 | 125 | 57 | 170 | 3.40e-15 | 68.2 |
| Msa1363590 | MtrunA17_Chr7g0270921 | 50.704 | 71 | 31 | 2 | 51 | 121 | 214 | 280 | 1.03e-14 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1363590 | AT2G31560.2 | 62.500 | 136 | 28 | 1 | 16 | 128 | 67 | 202 | 4.20e-53 | 166 |
| Msa1363590 | AT2G31560.1 | 62.500 | 136 | 28 | 1 | 16 | 128 | 67 | 202 | 4.20e-53 | 166 |
| Msa1363590 | AT2G31560.3 | 62.500 | 136 | 28 | 1 | 16 | 128 | 67 | 202 | 4.20e-53 | 166 |
| Msa1363590 | AT1G05870.4 | 61.111 | 144 | 24 | 3 | 17 | 128 | 46 | 189 | 9.01e-53 | 165 |
| Msa1363590 | AT1G05870.12 | 61.111 | 144 | 24 | 3 | 17 | 128 | 46 | 189 | 9.01e-53 | 165 |
| Msa1363590 | AT1G05870.8 | 61.111 | 144 | 24 | 3 | 17 | 128 | 46 | 189 | 9.01e-53 | 165 |
| Msa1363590 | AT1G05870.1 | 61.111 | 144 | 24 | 3 | 17 | 128 | 46 | 189 | 9.01e-53 | 165 |
| Msa1363590 | AT1G05870.7 | 61.111 | 144 | 24 | 3 | 17 | 128 | 46 | 189 | 9.01e-53 | 165 |
| Msa1363590 | AT1G05870.2 | 61.111 | 144 | 24 | 3 | 17 | 128 | 46 | 189 | 9.01e-53 | 165 |
| Msa1363590 | AT1G05870.10 | 61.111 | 144 | 24 | 3 | 17 | 128 | 46 | 189 | 9.01e-53 | 165 |
| Msa1363590 | AT1G05870.5 | 61.111 | 144 | 24 | 3 | 17 | 128 | 46 | 189 | 9.01e-53 | 165 |
| Msa1363590 | AT1G05870.9 | 61.111 | 144 | 24 | 3 | 17 | 128 | 46 | 189 | 9.01e-53 | 165 |
| Msa1363590 | AT1G05870.3 | 61.111 | 144 | 24 | 3 | 17 | 128 | 46 | 189 | 9.01e-53 | 165 |
| Msa1363590 | AT1G05870.6 | 61.111 | 144 | 24 | 3 | 17 | 128 | 46 | 189 | 9.01e-53 | 165 |
| Msa1363590 | AT1G05870.11 | 61.111 | 144 | 24 | 3 | 17 | 128 | 46 | 189 | 9.01e-53 | 165 |
| Msa1363590 | AT2G43340.1 | 62.698 | 126 | 22 | 2 | 28 | 128 | 64 | 189 | 3.61e-47 | 150 |
| Msa1363590 | AT3G22690.1 | 61.728 | 81 | 23 | 1 | 47 | 119 | 843 | 923 | 1.82e-24 | 97.8 |
| Msa1363590 | AT3G04700.1 | 39.316 | 117 | 37 | 2 | 39 | 124 | 74 | 187 | 4.28e-19 | 79.0 |
| Msa1363590 | AT5G28690.1 | 38.843 | 121 | 41 | 3 | 34 | 124 | 71 | 188 | 8.18e-19 | 78.2 |
| Msa1363590 | AT5G28690.3 | 38.843 | 121 | 41 | 3 | 34 | 124 | 71 | 188 | 8.18e-19 | 78.2 |
| Msa1363590 | AT5G28690.2 | 38.843 | 121 | 41 | 3 | 34 | 124 | 71 | 188 | 8.18e-19 | 78.2 |
| Msa1363590 | AT1G08790.1 | 40.741 | 108 | 34 | 3 | 47 | 124 | 78 | 185 | 1.09e-18 | 77.8 |
| Msa1363590 | AT3G50350.2 | 42.056 | 107 | 43 | 2 | 37 | 124 | 59 | 165 | 1.99e-18 | 76.6 |
| Msa1363590 | AT4G33985.1 | 45.370 | 108 | 39 | 2 | 41 | 128 | 43 | 150 | 3.21e-18 | 75.9 |
| Msa1363590 | AT3G04710.3 | 38.462 | 117 | 38 | 2 | 39 | 124 | 74 | 187 | 3.22e-18 | 79.7 |
| Msa1363590 | AT2G15590.2 | 38.318 | 107 | 47 | 3 | 41 | 128 | 40 | 146 | 7.97e-13 | 62.0 |
| Msa1363590 | AT3G50350.1 | 51.613 | 62 | 28 | 1 | 37 | 96 | 63 | 124 | 1.86e-12 | 61.2 |
| Msa1363590 | AT3G62070.1 | 46.667 | 75 | 30 | 3 | 52 | 121 | 150 | 219 | 3.06e-12 | 61.6 |
| Msa1363590 | AT2G46940.1 | 47.143 | 70 | 29 | 3 | 52 | 121 | 185 | 246 | 1.38e-11 | 60.1 |
| Msa1363590 | AT4G01670.1 | 49.296 | 71 | 30 | 2 | 51 | 121 | 172 | 236 | 9.63e-11 | 57.8 |
Find 32 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATGTTTGGATCTTGGTTT+TGG | 0.184629 | tig0018045:-32186 | Msa1363590:CDS |
| TTAAAGCAAGATTGAAATAT+TGG | 0.250359 | tig0018045:-32029 | Msa1363590:CDS |
| AATCTTGCTTTAACATCTTC+AGG | 0.331635 | tig0018045:+32039 | None:intergenic |
| TTCCAATTGGCAAATGAATC+AGG | 0.369053 | tig0018045:+32084 | None:intergenic |
| CTCCTGATTCATTTGCCAAT+TGG | 0.369305 | tig0018045:-32086 | Msa1363590:CDS |
| TGATGAGTTGAAGGGATGTT+TGG | 0.374718 | tig0018045:-32199 | Msa1363590:CDS |
| TAAAGCAAGATTGAAATATT+GGG | 0.375422 | tig0018045:-32028 | Msa1363590:CDS |
| TTGAAGGGATGTTTGGATCT+TGG | 0.389102 | tig0018045:-32192 | Msa1363590:CDS |
| TAGTTAGCTGCAGAGTTTAA+CGG | 0.390779 | tig0018045:+31987 | None:intergenic |
| GGGAGGGTGTTACATAGTTC+AGG | 0.412792 | tig0018045:+32144 | None:intergenic |
| ATGGAAGGTGGAGGTGTTGT+TGG | 0.413737 | tig0018045:-32354 | Msa1363590:CDS |
| CATCAAGATCCTCATCTGTT+AGG | 0.424926 | tig0018045:+32217 | None:intergenic |
| TGGACTTGAGATCTTCCAAT+TGG | 0.470698 | tig0018045:+32071 | None:intergenic |
| GGAGGTGTTGTTGGAAGAAG+TGG | 0.473169 | tig0018045:-32345 | Msa1363590:CDS |
| TGAGGATCTTGATGAGTTGA+AGG | 0.481670 | tig0018045:-32208 | Msa1363590:CDS |
| TAACAAAGGAGTAGTGTTGT+TGG | 0.483852 | tig0018045:-32286 | Msa1363590:CDS |
| GAATAACACAACTCTAGAGC+TGG | 0.494397 | tig0018045:+32123 | None:intergenic |
| ATAATGAGGAGGATCTTTGT+AGG | 0.495986 | tig0018045:-32248 | Msa1363590:CDS |
| AAGGAGTAGTGTTGTTGGAA+GGG | 0.511274 | tig0018045:-32281 | Msa1363590:CDS |
| AAAGGAGTAGTGTTGTTGGA+AGG | 0.535317 | tig0018045:-32282 | Msa1363590:CDS |
| ACATCTTCAGGATGATCACC+TGG | 0.572260 | tig0018045:+32051 | None:intergenic |
| AGGGTATGTTGATGATAATG+AGG | 0.584846 | tig0018045:-32262 | Msa1363590:CDS |
| AATAACACAACTCTAGAGCT+GGG | 0.590951 | tig0018045:+32124 | None:intergenic |
| AATTGGAAGATCTCAAGTCC+AGG | 0.596552 | tig0018045:-32069 | Msa1363590:CDS |
| GTTGTTGGAAGAAGTGGAAG+TGG | 0.596897 | tig0018045:-32339 | Msa1363590:CDS |
| ACACAACTCTAGAGCTGGGA+GGG | 0.600482 | tig0018045:+32128 | None:intergenic |
| GACGAAAAGCCTAACAGATG+AGG | 0.616832 | tig0018045:-32226 | Msa1363590:CDS |
| TTGTTGGAAGAAGTGGAAGT+GGG | 0.619030 | tig0018045:-32338 | Msa1363590:CDS |
| AATAAGAATAACAATAACAA+AGG | 0.625105 | tig0018045:-32300 | Msa1363590:CDS |
| GAGGATCTTGATGAGTTGAA+GGG | 0.630888 | tig0018045:-32207 | Msa1363590:CDS |
| GTATGTTGATGATAATGAGG+AGG | 0.675262 | tig0018045:-32259 | Msa1363590:CDS |
| AACACAACTCTAGAGCTGGG+AGG | 0.715725 | tig0018045:+32127 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAGAATAACAATAACAA+AGG | - | tig0018045:32044-32063 | Msa1363590:CDS | 15.0% |
| !! | TTAAAGCAAGATTGAAATAT+TGG | - | tig0018045:32315-32334 | Msa1363590:CDS | 20.0% |
| !! | TAAAGCAAGATTGAAATATT+GGG | - | tig0018045:32316-32335 | Msa1363590:CDS | 20.0% |
| AATCTTGCTTTAACATCTTC+AGG | + | tig0018045:32308-32327 | None:intergenic | 30.0% | |
| TAACAAAGGAGTAGTGTTGT+TGG | - | tig0018045:32058-32077 | Msa1363590:CDS | 35.0% | |
| !! | AGGGTATGTTGATGATAATG+AGG | - | tig0018045:32082-32101 | Msa1363590:CDS | 35.0% |
| !! | GTATGTTGATGATAATGAGG+AGG | - | tig0018045:32085-32104 | Msa1363590:CDS | 35.0% |
| ATAATGAGGAGGATCTTTGT+AGG | - | tig0018045:32096-32115 | Msa1363590:CDS | 35.0% | |
| !! | GATGTTTGGATCTTGGTTTT+GGG | - | tig0018045:32159-32178 | Msa1363590:CDS | 35.0% |
| !! | AATAACACAACTCTAGAGCT+GGG | + | tig0018045:32223-32242 | None:intergenic | 35.0% |
| TTCCAATTGGCAAATGAATC+AGG | + | tig0018045:32263-32282 | None:intergenic | 35.0% | |
| TTGTTGGAAGAAGTGGAAGT+GGG | - | tig0018045:32006-32025 | Msa1363590:CDS | 40.0% | |
| ! | AAAGGAGTAGTGTTGTTGGA+AGG | - | tig0018045:32062-32081 | Msa1363590:CDS | 40.0% |
| ! | AAGGAGTAGTGTTGTTGGAA+GGG | - | tig0018045:32063-32082 | Msa1363590:CDS | 40.0% |
| CATCAAGATCCTCATCTGTT+AGG | + | tig0018045:32130-32149 | None:intergenic | 40.0% | |
| ! | TGAGGATCTTGATGAGTTGA+AGG | - | tig0018045:32136-32155 | Msa1363590:CDS | 40.0% |
| ! | GAGGATCTTGATGAGTTGAA+GGG | - | tig0018045:32137-32156 | Msa1363590:CDS | 40.0% |
| ! | TGATGAGTTGAAGGGATGTT+TGG | - | tig0018045:32145-32164 | Msa1363590:CDS | 40.0% |
| TTGAAGGGATGTTTGGATCT+TGG | - | tig0018045:32152-32171 | Msa1363590:CDS | 40.0% | |
| ! | GGATGTTTGGATCTTGGTTT+TGG | - | tig0018045:32158-32177 | Msa1363590:CDS | 40.0% |
| !! | GAATAACACAACTCTAGAGC+TGG | + | tig0018045:32224-32243 | None:intergenic | 40.0% |
| CTCCTGATTCATTTGCCAAT+TGG | - | tig0018045:32258-32277 | Msa1363590:CDS | 40.0% | |
| TGGACTTGAGATCTTCCAAT+TGG | + | tig0018045:32276-32295 | None:intergenic | 40.0% | |
| AATTGGAAGATCTCAAGTCC+AGG | - | tig0018045:32275-32294 | Msa1363590:CDS | 40.0% | |
| GTTGTTGGAAGAAGTGGAAG+TGG | - | tig0018045:32005-32024 | Msa1363590:CDS | 45.0% | |
| GACGAAAAGCCTAACAGATG+AGG | - | tig0018045:32118-32137 | Msa1363590:CDS | 45.0% | |
| ACATCTTCAGGATGATCACC+TGG | + | tig0018045:32296-32315 | None:intergenic | 45.0% | |
| !! | ATGGAAGGTGGAGGTGTTGT+TGG | - | tig0018045:31990-32009 | Msa1363590:CDS | 50.0% |
| !! | GGAGGTGTTGTTGGAAGAAG+TGG | - | tig0018045:31999-32018 | Msa1363590:CDS | 50.0% |
| !! | GGGAGGGTGTTACATAGTTC+AGG | + | tig0018045:32203-32222 | None:intergenic | 50.0% |
| !! | ACACAACTCTAGAGCTGGGA+GGG | + | tig0018045:32219-32238 | None:intergenic | 50.0% |
| !! | AACACAACTCTAGAGCTGGG+AGG | + | tig0018045:32220-32239 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0018045 | gene | 31990 | 32376 | 31990 | ID=Msa1363590;Name=Msa1363590 |
| tig0018045 | mRNA | 31990 | 32376 | 31990 | ID=Msa1363590-mRNA-1;Parent=Msa1363590;Name=Msa1363590-mRNA-1;_AED=0.00;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|128 |
| tig0018045 | exon | 31990 | 32376 | 31990 | ID=Msa1363590-mRNA-1:exon:13632;Parent=Msa1363590-mRNA-1 |
| tig0018045 | CDS | 31990 | 32376 | 31990 | ID=Msa1363590-mRNA-1:cds;Parent=Msa1363590-mRNA-1 |
| Gene Sequence |
| Protein sequence |
