AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1371590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1371590 MtrunA17_Chr1g0157401 95.536 560 18 1 1 553 1 560 0.0 1098
Msa1371590 MtrunA17_Chr8g0353341 80.189 530 87 5 1 516 1 526 0.0 858
Msa1371590 MtrunA17_Chr1g0157431 94.000 350 14 1 1 343 1 350 0.0 675
Msa1371590 MtrunA17_Chr4g0028411 45.672 543 286 5 17 553 3 542 4.24e-162 472
Msa1371590 MtrunA17_Chr4g0039791 44.624 558 297 5 4 553 8 561 4.21e-161 470
Msa1371590 MtrunA17_Chr3g0116271 45.307 554 270 11 14 549 16 554 6.77e-160 467
Msa1371590 MtrunA17_Chr5g0409771 43.750 560 303 6 1 553 1 555 8.97e-159 464
Msa1371590 MtrunA17_Chr1g0197391 46.875 544 271 8 15 546 22 559 3.36e-158 463
Msa1371590 MtrunA17_Chr3g0116251 44.464 560 277 12 7 549 10 552 2.68e-157 461
Msa1371590 MtrunA17_Chr2g0327341 43.693 547 288 6 17 553 3 539 3.37e-153 449
Msa1371590 MtrunA17_Chr5g0409791 44.973 547 277 9 26 553 27 568 2.62e-152 448
Msa1371590 MtrunA17_Chr1g0180541 43.808 541 288 8 27 553 13 551 5.48e-145 429
Msa1371590 MtrunA17_Chr1g0180551 43.486 545 288 8 27 553 82 624 1.17e-142 425
Msa1371590 MtrunA17_Chr1g0186071 43.796 548 283 10 8 547 20 550 2.32e-139 415
Msa1371590 MtrunA17_Chr4g0072561 38.593 526 315 4 26 545 17 540 5.58e-118 360
Msa1371590 MtrunA17_Chr3g0102411 35.261 536 323 7 20 543 8 531 1.58e-116 356
Msa1371590 MtrunA17_Chr5g0395431 39.212 533 303 8 26 545 55 579 2.71e-116 356
Msa1371590 MtrunA17_Chr4g0000461 40.341 528 303 8 26 545 11 534 5.28e-114 349
Msa1371590 MtrunA17_Chr2g0334131 50.357 280 129 4 272 545 1 276 2.34e-86 269
Msa1371590 MtrunA17_Chr6g0471921 28.880 509 335 13 44 543 15 505 5.33e-52 185
Msa1371590 MtrunA17_Chr8g0370701 27.843 510 317 15 67 542 81 573 6.65e-47 173
Msa1371590 MtrunA17_Chr3g0109731 27.308 520 329 13 65 545 37 546 7.54e-46 169
Msa1371590 MtrunA17_Chr5g0396311 27.621 496 326 12 68 539 40 526 7.80e-44 163
Msa1371590 MtrunA17_Chr5g0433241 26.705 528 318 14 68 549 40 544 2.19e-41 157
Msa1371590 MtrunA17_Chr5g0396331 25.605 496 334 13 68 539 40 524 5.61e-39 150
Msa1371590 MtrunA17_Chr8g0370731 27.101 476 302 15 98 542 23 484 2.54e-37 144
Msa1371590 MtrunA17_Chr2g0301671 24.723 542 359 17 49 553 29 558 1.02e-36 144
Msa1371590 MtrunA17_Chr5g0433231 25.528 521 334 11 68 549 40 545 1.54e-36 143
Msa1371590 MtrunA17_Chr5g0433221 25.434 519 337 11 68 549 40 545 1.77e-36 143
Msa1371590 MtrunA17_Chr4g0068151 24.670 531 347 14 67 553 45 566 4.29e-35 139
Msa1371590 MtrunA17_Chr6g0471311 26.232 568 355 16 24 549 2 547 1.11e-34 137
Msa1371590 MtrunA17_Chr7g0252691 23.092 524 363 11 68 553 206 727 1.00e-33 136
Msa1371590 MtrunA17_Chr5g0406681 24.803 508 334 16 67 542 67 558 7.00e-32 129
Msa1371590 MtrunA17_Chr8g0377991 25.919 571 301 18 69 528 110 669 1.02e-29 124
Msa1371590 MtrunA17_Chr3g0121951 26.177 531 274 18 69 491 106 626 1.80e-28 120
Msa1371590 MtrunA17_Chr4g0028451 52.336 107 42 1 411 517 10 107 6.93e-28 107
Msa1371590 MtrunA17_Chr2g0292471 24.612 451 302 12 69 485 29 475 1.43e-25 110
Msa1371590 MtrunA17_Chr4g0004841 29.839 248 157 7 317 552 97 339 1.96e-25 107
Msa1371590 MtrunA17_Chr4g0004101 24.390 328 215 8 251 552 10 330 2.30e-24 104
Msa1371590 MtrunA17_Chr1g0205711 25.109 458 256 16 62 466 141 564 1.32e-21 99.0
Msa1371590 MtrunA17_Chr1g0160041 28.235 340 176 13 198 475 250 583 1.82e-19 92.4
Msa1371590 MtrunA17_Chr4g0033141 25.734 443 268 17 158 552 643 1072 3.71e-19 92.0
Msa1371590 MtrunA17_Chr2g0329161 24.943 441 266 17 158 547 638 1064 8.04e-19 90.9
Msa1371590 MtrunA17_Chr8g0388081 24.850 334 187 12 196 475 160 483 2.37e-18 88.6
Msa1371590 MtrunA17_Chr1g0194371 23.340 527 309 20 35 480 36 548 2.92e-18 88.6
Msa1371590 MtrunA17_Chr3g0121931 33.503 197 114 5 322 505 454 646 2.46e-16 82.4
Msa1371590 MtrunA17_Chr2g0334121 29.442 197 133 3 26 216 12 208 1.84e-15 75.5
Msa1371590 MtrunA17_Chr7g0270221 34.756 164 99 6 321 480 349 508 4.62e-15 78.2
Msa1371590 MtrunA17_Chr4g0034181 22.626 495 295 19 62 485 72 549 1.94e-14 76.3
Msa1371590 MtrunA17_Chr3g0124631 33.735 166 93 7 321 475 426 585 8.11e-14 74.3
Msa1371590 MtrunA17_Chr5g0396691 23.030 330 198 10 196 475 221 544 9.04e-14 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1371590 AT1G20510.1 74.630 540 134 2 14 551 4 542 0.0 828
Msa1371590 AT1G20500.1 66.302 549 170 5 13 553 9 550 0.0 738
Msa1371590 AT1G20510.2 73.673 471 121 2 14 482 4 473 0.0 711
Msa1371590 AT1G20480.1 61.429 560 202 4 4 553 10 565 0.0 708
Msa1371590 AT5G38120.1 61.580 557 193 7 9 553 3 550 0.0 693
Msa1371590 AT1G20510.3 73.051 449 118 2 14 460 4 451 0.0 675
Msa1371590 AT5G38120.3 60.269 521 186 7 9 517 3 514 0.0 635
Msa1371590 AT5G38120.4 60.494 486 171 7 9 482 3 479 0.0 590
Msa1371590 AT5G38120.2 60.494 486 171 7 9 482 3 479 0.0 590
Msa1371590 AT1G20490.2 60.047 428 154 5 13 430 9 429 1.92e-179 513
Msa1371590 AT1G20490.1 60.047 428 154 5 13 430 9 429 1.92e-179 513
Msa1371590 AT5G63380.1 43.162 563 286 8 8 549 5 554 2.85e-165 481
Msa1371590 AT4G19010.1 41.230 553 301 6 15 553 20 562 6.06e-156 457
Msa1371590 AT4G05160.1 44.059 547 291 7 17 553 3 544 1.33e-155 456
Msa1371590 AT3G21240.1 39.662 532 312 6 21 545 17 546 3.63e-121 368
Msa1371590 AT1G65060.1 40.832 529 298 6 26 545 34 556 1.98e-120 367
Msa1371590 AT1G51680.1 39.560 546 316 6 12 545 10 553 8.54e-113 347
Msa1371590 AT3G21230.1 38.817 541 310 9 22 545 24 560 4.39e-111 343
Msa1371590 AT1G62940.1 35.810 525 328 4 26 545 12 532 7.17e-103 320
Msa1371590 AT3G21230.3 37.308 520 301 10 22 524 24 535 6.87e-98 308
Msa1371590 AT1G51680.3 37.645 518 309 6 12 517 10 525 8.02e-98 307
Msa1371590 AT3G21240.2 37.712 472 285 6 21 485 17 486 1.60e-96 302
Msa1371590 AT3G21240.3 37.712 472 285 6 21 485 17 486 1.60e-96 302
Msa1371590 AT3G21230.2 37.317 477 278 9 22 481 24 496 8.15e-89 283
Msa1371590 AT1G51680.2 37.344 482 288 6 12 481 10 489 3.85e-88 281
Msa1371590 AT1G65060.2 37.444 446 264 6 26 462 34 473 1.00e-81 264
Msa1371590 AT3G21230.4 32.924 407 252 9 22 411 24 426 3.13e-52 184
Msa1371590 AT3G48990.1 28.008 482 335 10 67 543 30 504 1.44e-47 174
Msa1371590 AT1G65890.1 28.060 531 317 18 67 549 39 552 1.23e-46 172
Msa1371590 AT1G21530.1 27.514 527 323 15 62 549 37 543 3.07e-46 171
Msa1371590 AT1G20560.1 27.188 537 348 14 34 542 14 535 5.41e-46 170
Msa1371590 AT1G21530.2 27.238 525 329 14 62 549 37 545 1.02e-45 169
Msa1371590 AT1G77240.1 26.515 528 338 15 58 551 32 543 3.14e-45 167
Msa1371590 AT1G65880.1 28.839 534 310 20 67 549 39 553 3.90e-45 168
Msa1371590 AT1G21540.1 27.746 519 323 14 69 551 46 548 1.89e-44 166
Msa1371590 AT1G76290.1 28.101 516 318 18 67 549 39 534 2.45e-44 165
Msa1371590 AT1G66120.1 27.810 525 323 15 67 551 39 547 6.03e-44 164
Msa1371590 AT5G16370.1 26.628 522 326 14 74 553 43 549 2.01e-41 157
Msa1371590 AT2G17650.1 27.321 560 343 20 37 549 55 597 3.21e-40 154
Msa1371590 AT5G16340.1 26.796 515 321 14 74 547 43 542 1.64e-39 152
Msa1371590 AT1G20560.2 27.692 455 292 12 116 542 12 457 3.46e-39 149
Msa1371590 AT1G30520.2 27.155 464 292 11 64 496 34 482 8.14e-38 147
Msa1371590 AT1G30520.1 27.155 464 292 11 64 496 34 482 8.14e-38 147
Msa1371590 AT1G75960.1 26.488 521 324 14 68 549 41 541 8.88e-37 144
Msa1371590 AT1G68270.1 24.952 521 308 16 67 545 39 518 2.44e-33 134
Msa1371590 AT3G16170.1 24.672 381 245 9 197 547 234 602 3.27e-32 131
Msa1371590 AT5G36880.6 22.761 536 362 13 60 553 144 669 3.33e-32 132
Msa1371590 AT5G36880.3 22.761 536 362 13 60 553 144 669 3.33e-32 132
Msa1371590 AT5G36880.5 22.761 536 362 13 60 553 144 669 3.33e-32 132
Msa1371590 AT5G36880.1 22.761 536 362 13 60 553 144 669 3.33e-32 132
Msa1371590 AT5G36880.2 22.948 536 361 14 60 553 194 719 4.57e-32 131
Msa1371590 AT3G16910.1 23.654 520 344 16 67 545 47 554 1.02e-31 129
Msa1371590 AT1G30520.3 25.843 445 281 12 64 477 34 460 1.51e-30 125
Msa1371590 AT1G30520.4 25.843 445 281 12 64 477 34 460 1.51e-30 125
Msa1371590 AT5G36880.4 21.957 460 312 11 60 481 144 594 3.63e-25 110
Msa1371590 AT5G27600.1 27.760 317 172 9 197 466 261 567 2.11e-22 102
Msa1371590 AT3G05970.1 28.707 317 169 9 197 466 261 567 5.36e-20 94.4
Msa1371590 AT1G64400.1 22.515 493 303 19 55 478 65 547 1.59e-19 92.8
Msa1371590 AT1G77590.2 27.874 348 167 14 198 475 100 433 2.91e-19 91.7
Msa1371590 AT1G77590.1 27.874 348 167 14 198 475 246 579 5.46e-19 91.3
Msa1371590 AT2G47240.4 26.571 350 200 12 180 480 147 488 6.43e-19 90.9
Msa1371590 AT2G47240.1 26.571 350 200 12 180 480 206 547 7.21e-19 90.9
Msa1371590 AT2G47240.2 26.571 350 200 12 180 480 206 547 7.21e-19 90.9
Msa1371590 AT2G47240.3 26.571 350 200 12 180 480 206 547 7.21e-19 90.9
Msa1371590 AT1G49430.1 25.000 344 199 13 193 485 219 554 1.02e-16 84.0
Msa1371590 AT4G11030.1 23.548 310 174 10 198 454 224 523 7.10e-15 78.2
Msa1371590 AT3G23790.1 32.632 190 103 6 322 490 455 640 4.15e-13 72.8
Msa1371590 AT3G23790.1 24.797 246 152 9 68 282 111 354 2.55e-12 70.1
Msa1371590 AT4G14070.1 29.600 250 145 8 322 546 471 714 5.64e-12 68.9

Find 179 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTGCCCAGCCTCCTTATT+TGG 0.136723 tig0019233:-179749 None:intergenic
TCGCTTCATACGGTGGTTTC+TGG 0.138183 tig0019233:+177941 Msa1371590:CDS
TGATAGGTTTCTATCGAATT+TGG 0.177748 tig0019233:+180091 Msa1371590:three_prime_UTR
CTGCTGCAAAACCCACTTCT+TGG 0.251921 tig0019233:-177038 None:intergenic
GTTGGAGTCAAAAGAGAAAA+AGG 0.268019 tig0019233:+176820 None:intergenic
ACCACTTGTGCCGCCGATAC+TGG 0.271545 tig0019233:+177868 Msa1371590:CDS
GTAAGTATCTTTCAAGGTTA+TGG 0.275288 tig0019233:+178019 Msa1371590:CDS
TGGAGCTTCTACTGAGTCTT+TGG 0.282200 tig0019233:+178063 Msa1371590:CDS
TTCTATCGAATTTGGTTAGA+TGG 0.317747 tig0019233:+180099 Msa1371590:three_prime_UTR
TTGTGTAGTATCTATCAAAC+TGG 0.323309 tig0019233:-176938 None:intergenic
TGTTGTTGATGATGAGTTGC+TGG 0.335506 tig0019233:-177495 None:intergenic
GTTCCGATGTTTCATATATA+CGG 0.342157 tig0019233:+177731 Msa1371590:CDS
TGCTATGGGGCTTCTTGCTA+TGG 0.342915 tig0019233:+177766 Msa1371590:CDS
TTAACTGAATCCTCTGGAAT+TGG 0.351281 tig0019233:+178043 Msa1371590:CDS
AGAATTGTCACATCACAAAT+TGG 0.354196 tig0019233:-180478 None:intergenic
AGCTCACCAGTCCGATTAAC+TGG 0.354985 tig0019233:-178172 None:intergenic
CTCTACTCATCAGGAACAAC+TGG 0.359656 tig0019233:+177599 Msa1371590:CDS
GCAGACAACAGGGAAGTAAA+TGG 0.362942 tig0019233:-177297 None:intergenic
TAGTTAATTAACTAAAGTTT+AGG 0.372221 tig0019233:+180448 Msa1371590:three_prime_UTR
CAAGTTGTAATGGCTAGATT+TGG 0.372468 tig0019233:+177671 Msa1371590:CDS
GGAGGATGACGTCACCTTTA+CGG 0.372564 tig0019233:-177259 None:intergenic
TTATATCAGGTATCCAAATA+AGG 0.373402 tig0019233:+179736 Msa1371590:intron
CATTAAGTACAAGGGATATC+AGG 0.379179 tig0019233:+179318 Msa1371590:CDS
AAGGGAAGCAGGAAGCATTC+AGG 0.399635 tig0019233:-180187 None:intergenic
GGTGAATTGGTGGCCGGTGG+AGG 0.402061 tig0019233:-177177 None:intergenic
ATGACGGAGAGGCAGACAAC+AGG 0.402334 tig0019233:-177308 None:intergenic
AAGGCTGGAAGTAGCTTGTC+AGG 0.410549 tig0019233:+179794 Msa1371590:CDS
TTAATCGGACTGGTGAGCTT+TGG 0.414553 tig0019233:+178176 Msa1371590:CDS
TGGAGTTGGGAGAGAGGAGG+AGG 0.417129 tig0019233:-177277 None:intergenic
GAATTTAGAGAGAACAACTA+TGG 0.419281 tig0019233:-177795 None:intergenic
AAGAAACGAACCTTTCATAA+TGG 0.419398 tig0019233:-178212 None:intergenic
AGTAAATGGAGTTGGGAGAG+AGG 0.420482 tig0019233:-177283 None:intergenic
TTTGTGACAAAATAAAGAAA+TGG 0.421519 tig0019233:-180135 None:intergenic
TGGTTTGGTGGAAGTGGAAG+GGG 0.421746 tig0019233:-177089 None:intergenic
ACTGGTGAGCTTTGGCTTCG+TGG 0.421933 tig0019233:+178184 Msa1371590:CDS
AGTGAAAGCAAGAACAGGTT+TGG 0.423538 tig0019233:-177405 None:intergenic
ATCCATCTGGTAAAATTCTT+CGG 0.423771 tig0019233:+180013 Msa1371590:CDS
AAAGAGTGGTTTGGTGGAAG+TGG 0.424298 tig0019233:-177095 None:intergenic
CCTTGAAAGATACTTACATT+AGG 0.428705 tig0019233:-178013 None:intergenic
AGAGATTAAGATTAAGAAGA+TGG 0.429051 tig0019233:+176974 Msa1371590:exon
TCCAAATAAGGAGGCTGGGC+AGG 0.431867 tig0019233:+179748 Msa1371590:CDS
CCTAATGTAAGTATCTTTCA+AGG 0.432845 tig0019233:+178013 Msa1371590:CDS
CTCAGCCGTTACCAGTTAAT+CGG 0.436385 tig0019233:+178161 Msa1371590:CDS
GGTCTTGCAGTGTTTGCTAT+GGG 0.437424 tig0019233:+177752 Msa1371590:CDS
CGGTCTTGCAGTGTTTGCTA+TGG 0.438320 tig0019233:+177751 Msa1371590:CDS
GCTATGGGGCTTCTTGCTAT+GGG 0.440856 tig0019233:+177767 Msa1371590:CDS
CTACAGCACGCCAGAGTTGT+TGG 0.441513 tig0019233:-177202 None:intergenic
CTTGTCAGGAAGCCAAGTTA+TGG 0.458124 tig0019233:+179808 Msa1371590:CDS
TTGGAAGAGAGTCGTAAGTA+TGG 0.463115 tig0019233:+178082 Msa1371590:CDS
AGGCGTCAATGAAGGCGATA+TGG 0.463838 tig0019233:-177157 None:intergenic
GGGGCTTGTGTCTGCTTCCA+CGG 0.471317 tig0019233:+178111 Msa1371590:CDS
TATGGTTTAACTGAATCCTC+TGG 0.471540 tig0019233:+178037 Msa1371590:CDS
GTGGTTTGGTGGAAGTGGAA+GGG 0.473586 tig0019233:-177090 None:intergenic
AGAGTTGTTGGTAGGTGAAT+TGG 0.474858 tig0019233:-177190 None:intergenic
AGGTATCCAAATAAGGAGGC+TGG 0.481674 tig0019233:+179743 Msa1371590:intron
TTCACTTCCAATCATTCTCA+TGG 0.482486 tig0019233:+177466 Msa1371590:CDS
TAACCAACCCTCTGGAGTAA+TGG 0.484888 tig0019233:-179227 None:intergenic
CGATTTCGCGAGATGTATTG+AGG 0.485277 tig0019233:-177364 None:intergenic
GCGAGATGTATTGAGGGGAT+TGG 0.486320 tig0019233:-177357 None:intergenic
AACAAAGCCTCTAGTTCTGC+TGG 0.486762 tig0019233:-179529 None:intergenic
CACTTCCTCACTCTCCAACA+TGG 0.488973 tig0019233:+177232 Msa1371590:CDS
GCCGGTGGAGGCGTCAATGA+AGG 0.490250 tig0019233:-177165 None:intergenic
AGTGGTTTGGTGGAAGTGGA+AGG 0.490859 tig0019233:-177091 None:intergenic
GCATATGTGGTAAGGAAGGC+TGG 0.491572 tig0019233:+179779 Msa1371590:CDS
TTATTCTCTATAAGGGAAGC+AGG 0.492146 tig0019233:-180198 None:intergenic
ATTAAGAAGATGGAGGTAAA+CGG 0.492454 tig0019233:+176984 Msa1371590:exon
TGTATTGAGGGGATTGGTGG+TGG 0.493354 tig0019233:-177351 None:intergenic
CGATGGATTCTTATTTGTCG+TGG 0.494381 tig0019233:+179279 Msa1371590:CDS
GTGGTGTCCATGAGAATGAT+TGG 0.496058 tig0019233:-177473 None:intergenic
AACAGGGAAGTAAATGGAGT+TGG 0.497843 tig0019233:-177291 None:intergenic
AAGGTAGTAAATAAATAATG+AGG 0.498509 tig0019233:+176839 Msa1371590:five_prime_UTR
CTGCAACAAAATCCATAACT+TGG 0.502133 tig0019233:-179820 None:intergenic
TGATTTGAGTTCGCTTCATA+CGG 0.505643 tig0019233:+177931 Msa1371590:CDS
TTGGTAGGTGAATTGGTGGC+CGG 0.506146 tig0019233:-177183 None:intergenic
GCCACACTACTCTACTCATC+AGG 0.506955 tig0019233:+177590 Msa1371590:CDS
AGTCGTAAGTATGGTACGGC+GGG 0.509532 tig0019233:+178091 Msa1371590:CDS
CGGCGGCACAAGTGGTAAGA+AGG 0.513016 tig0019233:-177861 None:intergenic
CATCGCTATGAGATTCTTGT+GGG 0.513470 tig0019233:-177645 None:intergenic
GCCTTCATTGACGCCTCCAC+CGG 0.515250 tig0019233:+177164 Msa1371590:CDS
GAGTGAATGAATGATGATAT+TGG 0.515321 tig0019233:+176880 Msa1371590:five_prime_UTR
TGGAAGTGAAGGTGATGCTT+GGG 0.520153 tig0019233:-177453 None:intergenic
GGAGTAGTGAAAGCAAGAAC+AGG 0.520555 tig0019233:-177410 None:intergenic
GAAGATACTACTCCTTTGCT+TGG 0.520592 tig0019233:-177623 None:intergenic
ACTTCCTCACTCTCCAACAT+GGG 0.520727 tig0019233:+177233 Msa1371590:CDS
TGCCTCTCCGTCATGTCCCT+CGG 0.521898 tig0019233:+177317 Msa1371590:CDS
TTCCTTACCACATATGCCAT+TGG 0.522529 tig0019233:-179773 None:intergenic
AGCGATGGTTCAAGTTGTAA+TGG 0.525827 tig0019233:+177661 Msa1371590:CDS
ACAGGGAAGTAAATGGAGTT+GGG 0.526332 tig0019233:-177290 None:intergenic
AGGTAGTAAATAAATAATGA+GGG 0.527522 tig0019233:+176840 Msa1371590:five_prime_UTR
TCCGTTACTTCTTTACTCAA+AGG 0.528090 tig0019233:-177971 None:intergenic
TTAACTAAAGTTTAGGCATG+AGG 0.532817 tig0019233:+180455 Msa1371590:three_prime_UTR
GTTTGCTATATTGATAGCGA+TGG 0.538596 tig0019233:+179262 Msa1371590:CDS
TTGGAAGTGAAGGTGATGCT+TGG 0.539417 tig0019233:-177454 None:intergenic
ATACAAAAGAATTCGAAAAG+TGG 0.543360 tig0019233:+179966 Msa1371590:CDS
TTCCAATGGCATATGTGGTA+AGG 0.544112 tig0019233:+179771 Msa1371590:CDS
CTTCATACGGTGGTTTCTGG+TGG 0.547015 tig0019233:+177944 Msa1371590:CDS
ATCTGGTAAAATTCTTCGGA+AGG 0.547613 tig0019233:+180017 Msa1371590:CDS
GGAGGCTGGGCAGGTTCCAA+TGG 0.547827 tig0019233:+179757 Msa1371590:CDS
ACAGCAGCGTCTACAATAGC+AGG 0.550475 tig0019233:-179562 None:intergenic
GTAGGTGAATTGGTGGCCGG+TGG 0.552038 tig0019233:-177180 None:intergenic
ACCTTTACGGATCCCCATGT+TGG 0.552224 tig0019233:-177246 None:intergenic
GAAAGAGCTCATTAAGTACA+AGG 0.552506 tig0019233:+179309 Msa1371590:CDS
GCATCTAAAGAGTGGTTTGG+TGG 0.553390 tig0019233:-177101 None:intergenic
CGTGGTCCCACCATTATGAA+AGG 0.554145 tig0019233:+178202 Msa1371590:CDS
TCCAACATGGGGATCCGTAA+AGG 0.554642 tig0019233:+177245 Msa1371590:CDS
GCAGGTTCCAATGGCATATG+TGG 0.556198 tig0019233:+179766 Msa1371590:CDS
ACCAGTATCGGCGGCACAAG+TGG 0.558595 tig0019233:-177869 None:intergenic
AAACGAACCTTTCATAATGG+TGG 0.559397 tig0019233:-178209 None:intergenic
TTCAGCATAGCAACCAGTAT+CGG 0.561145 tig0019233:-177881 None:intergenic
CCAGTCCGATTAACTGGTAA+CGG 0.565679 tig0019233:-178166 None:intergenic
GGTATCCAAATAAGGAGGCT+GGG 0.569081 tig0019233:+179744 Msa1371590:intron
CCATCGCTATGAGATTCTTG+TGG 0.569419 tig0019233:-177646 None:intergenic
GTGGCATCTAAAGAGTGGTT+TGG 0.570177 tig0019233:-177104 None:intergenic
CCGTTACCAGTTAATCGGAC+TGG 0.573347 tig0019233:+178166 Msa1371590:CDS
TTGAGTAAAGAAGTAACGGA+AGG 0.574145 tig0019233:+177974 Msa1371590:CDS
AGATGTATTGAGGGGATTGG+TGG 0.576476 tig0019233:-177354 None:intergenic
ATCTCCTCCCGCGCTCATCA+CGG 0.577531 tig0019233:+177134 Msa1371590:CDS
GATTAAGATTAAGAAGATGG+AGG 0.581479 tig0019233:+176977 Msa1371590:exon
GAAGCATTCAGGAATGCAGC+AGG 0.582823 tig0019233:-180176 None:intergenic
TCCTTTGAGTAAAGAAGTAA+CGG 0.584099 tig0019233:+177970 Msa1371590:CDS
GATTTCGCGAGATGTATTGA+GGG 0.584385 tig0019233:-177363 None:intergenic
AATTCACCTACCAACAACTC+TGG 0.585675 tig0019233:+177192 Msa1371590:CDS
AACGAACCTTTCATAATGGT+GGG 0.587290 tig0019233:-178208 None:intergenic
GATATGGCCGTGATGAGCGC+GGG 0.587698 tig0019233:-177141 None:intergenic
AAATGGAGTTGGGAGAGAGG+AGG 0.592217 tig0019233:-177280 None:intergenic
TGAGTAAAGAAGTAACGGAA+GGG 0.592240 tig0019233:+177975 Msa1371590:CDS
GTAGTAAATAAATAATGAGG+GGG 0.595227 tig0019233:+176842 Msa1371590:five_prime_UTR
GTTGTTGGTAGGTGAATTGG+TGG 0.596032 tig0019233:-177187 None:intergenic
AATGGCATATGTGGTAAGGA+AGG 0.598196 tig0019233:+179775 Msa1371590:CDS
GTCTTGCAGTGTTTGCTATG+GGG 0.598502 tig0019233:+177753 Msa1371590:CDS
CGATATGGCCGTGATGAGCG+CGG 0.598855 tig0019233:-177142 None:intergenic
TGACGGAGAGGCAGACAACA+GGG 0.598859 tig0019233:-177307 None:intergenic
GTTGGAGAGTGAGGAAGTGA+CGG 0.598886 tig0019233:-177228 None:intergenic
CGACCATTACTCCAGAGGGT+TGG 0.603546 tig0019233:+179224 Msa1371590:CDS
GATAGCGCCGAGGGACATGA+CGG 0.604148 tig0019233:-177324 None:intergenic
AGAGTGATCGATCCAAGAAG+TGG 0.604701 tig0019233:+177026 Msa1371590:CDS
AGTGACTGAGCGCATCAACC+AGG 0.608319 tig0019233:+177559 Msa1371590:CDS
TGTTGATGATGAGTTGCTGG+TGG 0.609037 tig0019233:-177492 None:intergenic
ATGGCCGTGATGAGCGCGGG+AGG 0.610511 tig0019233:-177138 None:intergenic
CCACAAGAATCTCATAGCGA+TGG 0.611191 tig0019233:+177646 Msa1371590:CDS
GGTCCCTCCAGCAGAACTAG+AGG 0.614140 tig0019233:+179522 Msa1371590:intron
TTTGAGTTCGCTTCATACGG+TGG 0.616174 tig0019233:+177934 Msa1371590:CDS
GATCCCCATGTTGGAGAGTG+AGG 0.617341 tig0019233:-177237 None:intergenic
GGTGGTGATGATAGCGCCGA+GGG 0.619275 tig0019233:-177333 None:intergenic
AGAGAGTCGTAAGTATGGTA+CGG 0.623595 tig0019233:+178087 Msa1371590:CDS
AGCGCGGGAGGAGATGAAAG+TGG 0.628660 tig0019233:-177126 None:intergenic
GGTAGTAAATAAATAATGAG+GGG 0.629942 tig0019233:+176841 Msa1371590:five_prime_UTR
TGTGTAGTATCTATCAAACT+GGG 0.632023 tig0019233:-176937 None:intergenic
GGAACAACTGGACCAAGCAA+AGG 0.634924 tig0019233:+177611 Msa1371590:CDS
TCAGTCACTCTACTCAACTC+AGG 0.638010 tig0019233:-177545 None:intergenic
GTAGTGTGGCTGTATCATCC+TGG 0.639019 tig0019233:-177577 None:intergenic
ATGAGAATGATTGGAAGTGA+AGG 0.639263 tig0019233:-177464 None:intergenic
AAAGCCTCTAGTTCTGCTGG+AGG 0.643862 tig0019233:-179526 None:intergenic
AGCATAGCAACCAGTATCGG+CGG 0.644393 tig0019233:-177878 None:intergenic
GGCAGGTTATTTCAGTAACG+AGG 0.647450 tig0019233:+179189 Msa1371590:intron
ACATCGACCATTACTCCAGA+GGG 0.647619 tig0019233:+179220 Msa1371590:CDS
AAAGAGCTCATTAAGTACAA+GGG 0.651964 tig0019233:+179310 Msa1371590:CDS
GAGTGATCGATCCAAGAAGT+GGG 0.652473 tig0019233:+177027 Msa1371590:CDS
AGACCGTATATATGAAACAT+CGG 0.653796 tig0019233:-177734 None:intergenic
GCTTGTGTCTGCTTCCACGG+AGG 0.655096 tig0019233:+178114 Msa1371590:CDS
CTCTGGAGTAATGGTCGATG+TGG 0.656546 tig0019233:-179218 None:intergenic
AAGCCTCTAGTTCTGCTGGA+GGG 0.656701 tig0019233:-179525 None:intergenic
TAGTAAATAAATAATGAGGG+GGG 0.661298 tig0019233:+176843 Msa1371590:five_prime_UTR
ATTTCGCGAGATGTATTGAG+GGG 0.663874 tig0019233:-177362 None:intergenic
CTTCCTCACTCTCCAACATG+GGG 0.667378 tig0019233:+177234 Msa1371590:CDS
GCGGGAGGAGATGAAAGTGG+TGG 0.667975 tig0019233:-177123 None:intergenic
AGGTTATTTCAGTAACGAGG+AGG 0.670840 tig0019233:+179192 Msa1371590:intron
CGCCGAGGGACATGACGGAG+AGG 0.671164 tig0019233:-177319 None:intergenic
AAGTGGTGGCATCTAAAGAG+TGG 0.673146 tig0019233:-177109 None:intergenic
TATCAGGTATCCAAATAAGG+AGG 0.676684 tig0019233:+179739 Msa1371590:intron
CACATCGACCATTACTCCAG+AGG 0.683319 tig0019233:+179219 Msa1371590:CDS
TCCTGATGAGTAGAGTAGTG+TGG 0.691172 tig0019233:-177591 None:intergenic
GTCGTAAGTATGGTACGGCG+GGG 0.691371 tig0019233:+178092 Msa1371590:CDS
AGCACGCCAGAGTTGTTGGT+AGG 0.696936 tig0019233:-177198 None:intergenic
AGTAGAAGCTCCAATTCCAG+AGG 0.697004 tig0019233:-178053 None:intergenic
TGGTGGTGATGATAGCGCCG+AGG 0.700534 tig0019233:-177334 None:intergenic
GTCAACAATCATAGCCTCCG+TGG 0.700669 tig0019233:-178128 None:intergenic
GAGTCGTAAGTATGGTACGG+CGG 0.704214 tig0019233:+178090 Msa1371590:CDS
TGAATGATGATATTGGACGA+CGG 0.752090 tig0019233:+176887 Msa1371590:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTAATTAATTAAAAA+AGG - tig0019233:178370-178389 None:intergenic 0.0%
!!! AAGCAAATATTATTTTTTTT+AGG + tig0019233:180295-180314 Msa1371590:three_prime_UTR 10.0%
!!! AATATTATTTTTTTTAGGTT+TGG + tig0019233:180300-180319 Msa1371590:three_prime_UTR 10.0%
!! GAAAAAAATTAAAGTTGTAT+TGG - tig0019233:178572-178591 None:intergenic 15.0%
!! ATTAATTTATATTCTTTGGT+AGG - tig0019233:179599-179618 None:intergenic 15.0%
!!! AATTTTTTTCAATATATAGG+TGG + tig0019233:179939-179958 Msa1371590:intron 15.0%
!!! AACATTTTTATTCTCTATAA+GGG - tig0019233:180208-180227 None:intergenic 15.0%
!!! TCAATTTTAGCAATATTATA+CGG - tig0019233:180363-180382 None:intergenic 15.0%
!! TAGTTAATTAACTAAAGTTT+AGG + tig0019233:180448-180467 Msa1371590:three_prime_UTR 15.0%
!! AAGGTAGTAAATAAATAATG+AGG + tig0019233:176839-176858 Msa1371590:five_prime_UTR 20.0%
!! AGGTAGTAAATAAATAATGA+GGG + tig0019233:176840-176859 Msa1371590:five_prime_UTR 20.0%
!! ATAAACAATTCAGATTAGAT+TGG + tig0019233:178297-178316 Msa1371590:intron 20.0%
!! TAATTAAAATATTGAACACG+AGG + tig0019233:178378-178397 Msa1371590:intron 20.0%
!!! TGTCTTTTTCTAATAAAGTA+AGG + tig0019233:178648-178667 Msa1371590:intron 20.0%
!! AAATGGAATGAAATAATCAT+TGG - tig0019233:178697-178716 None:intergenic 20.0%
!!! ATCGATATTCTAAAAAATGT+GGG - tig0019233:178807-178826 None:intergenic 20.0%
!!! TATCGATATTCTAAAAAATG+TGG - tig0019233:178808-178827 None:intergenic 20.0%
!! AGTAAAATGTCAAAATTGTT+AGG - tig0019233:178902-178921 None:intergenic 20.0%
!!! GTTACTTTTTTTATGTCTAA+TGG - tig0019233:179001-179020 None:intergenic 20.0%
!!! TTTTGTGTGTATATTATATC+AGG + tig0019233:179723-179742 Msa1371590:intron 20.0%
!! TTTGTGACAAAATAAAGAAA+TGG - tig0019233:180138-180157 None:intergenic 20.0%
!!! CAACATTTTTATTCTCTATA+AGG - tig0019233:180209-180228 None:intergenic 20.0%
!! TATAGAGAATAAAAATGTTG+TGG + tig0019233:180209-180228 Msa1371590:three_prime_UTR 20.0%
! GGTAGTAAATAAATAATGAG+GGG + tig0019233:176841-176860 Msa1371590:five_prime_UTR 25.0%
! GTAGTAAATAAATAATGAGG+GGG + tig0019233:176842-176861 Msa1371590:five_prime_UTR 25.0%
! TAGTAAATAAATAATGAGGG+GGG + tig0019233:176843-176862 Msa1371590:five_prime_UTR 25.0%
! AGAGATTAAGATTAAGAAGA+TGG + tig0019233:176974-176993 Msa1371590:exon 25.0%
! AATTATAGCATATAAACTCG+TGG - tig0019233:178346-178365 None:intergenic 25.0%
! TTATTACAAACTCACACAAA+TGG + tig0019233:178846-178865 Msa1371590:intron 25.0%
!! TTTTAGTGTTATAAACACCT+TGG - tig0019233:179048-179067 None:intergenic 25.0%
! CACTAAAATAATAGATGCAA+AGG + tig0019233:179060-179079 Msa1371590:intron 25.0%
! ATATAAGCTCACATAATTGT+TGG + tig0019233:179122-179141 Msa1371590:intron 25.0%
!!! CATAATTGTTGGTTCTATTA+TGG + tig0019233:179133-179152 Msa1371590:intron 25.0%
! GTAAACAGTAATTTATCTCT+GGG - tig0019233:179414-179433 None:intergenic 25.0%
! TCTTTATTACTATTGTGTGT+CGG - tig0019233:179436-179455 None:intergenic 25.0%
!! TGTTTTATGGTTATTGTTAC+AGG + tig0019233:179501-179520 Msa1371590:intron 25.0%
! TTATATCAGGTATCCAAATA+AGG + tig0019233:179736-179755 Msa1371590:intron 25.0%
!!! ACTCTTTTTTATTAGATGCT+TGG + tig0019233:179881-179900 Msa1371590:intron 25.0%
!!! CTCTTTTTTATTAGATGCTT+GGG + tig0019233:179882-179901 Msa1371590:intron 25.0%
!! ACTTTTCGAATTCTTTTGTA+TGG - tig0019233:179967-179986 None:intergenic 25.0%
! ATACAAAAGAATTCGAAAAG+TGG + tig0019233:179966-179985 Msa1371590:CDS 25.0%
!!! ATTTTACCAGATGGATTTTT+GGG - tig0019233:180009-180028 None:intergenic 25.0%
!!! AATTTTACCAGATGGATTTT+TGG - tig0019233:180010-180029 None:intergenic 25.0%
!! CAGTTGAACTTTTAAATGAT+AGG + tig0019233:180075-180094 Msa1371590:three_prime_UTR 25.0%
GAGTGAATGAATGATGATAT+TGG + tig0019233:176880-176899 Msa1371590:five_prime_UTR 30.0%
TGTGTAGTATCTATCAAACT+GGG - tig0019233:176940-176959 None:intergenic 30.0%
TTGTGTAGTATCTATCAAAC+TGG - tig0019233:176941-176960 None:intergenic 30.0%
GATTAAGATTAAGAAGATGG+AGG + tig0019233:176977-176996 Msa1371590:exon 30.0%
ATTAAGAAGATGGAGGTAAA+CGG + tig0019233:176984-177003 Msa1371590:exon 30.0%
GTTCCGATGTTTCATATATA+CGG + tig0019233:177731-177750 Msa1371590:CDS 30.0%
AGACCGTATATATGAAACAT+CGG - tig0019233:177737-177756 None:intergenic 30.0%
GAATTTAGAGAGAACAACTA+TGG - tig0019233:177798-177817 None:intergenic 30.0%
TCCTTTGAGTAAAGAAGTAA+CGG + tig0019233:177970-177989 Msa1371590:CDS 30.0%
CCTTGAAAGATACTTACATT+AGG - tig0019233:178016-178035 None:intergenic 30.0%
CCTAATGTAAGTATCTTTCA+AGG + tig0019233:178013-178032 Msa1371590:CDS 30.0%
GTAAGTATCTTTCAAGGTTA+TGG + tig0019233:178019-178038 Msa1371590:CDS 30.0%
AAGAAACGAACCTTTCATAA+TGG - tig0019233:178215-178234 None:intergenic 30.0%
TGAATTGCTAAACAAGTTTC+CGG - tig0019233:178409-178428 None:intergenic 30.0%
!! AAGTGACACTCATTTTGAAA+CGG - tig0019233:178506-178525 None:intergenic 30.0%
AAACTCACACAAATGGATAT+TGG + tig0019233:178853-178872 Msa1371590:intron 30.0%
AACTCACACAAATGGATATT+GGG + tig0019233:178854-178873 Msa1371590:intron 30.0%
GCTAATTAAAATGCAAGACA+TGG + tig0019233:179158-179177 Msa1371590:intron 30.0%
ATTAAAATGCAAGACATGGT+TGG + tig0019233:179162-179181 Msa1371590:intron 30.0%
AAAGAGCTCATTAAGTACAA+GGG + tig0019233:179310-179329 Msa1371590:CDS 30.0%
GGTAAACAGTAATTTATCTC+TGG - tig0019233:179415-179434 None:intergenic 30.0%
CGTAAATATTGTCAAACTCA+TGG + tig0019233:179463-179482 Msa1371590:intron 30.0%
!!! GTTCTCTTGCTTTTGTTTTA+TGG + tig0019233:179488-179507 Msa1371590:intron 30.0%
TATATTCTTTGGTAGGCTTA+CGG - tig0019233:179592-179611 None:intergenic 30.0%
TGATTGATAAGAAGGAATAC+TGG - tig0019233:179690-179709 None:intergenic 30.0%
TATACACACAAAACATTCAG+CGG - tig0019233:179716-179735 None:intergenic 30.0%
ATCCATCTGGTAAAATTCTT+CGG + tig0019233:180013-180032 Msa1371590:CDS 30.0%
TGATAGGTTTCTATCGAATT+TGG + tig0019233:180091-180110 Msa1371590:three_prime_UTR 30.0%
TTCTATCGAATTTGGTTAGA+TGG + tig0019233:180099-180118 Msa1371590:three_prime_UTR 30.0%
GAATAAAAATGTTGTGGTCT+TGG + tig0019233:180215-180234 Msa1371590:three_prime_UTR 30.0%
TTAACTAAAGTTTAGGCATG+AGG + tig0019233:180455-180474 Msa1371590:three_prime_UTR 30.0%
AGAATTGTCACATCACAAAT+TGG - tig0019233:180481-180500 None:intergenic 30.0%
TGAATGATGATATTGGACGA+CGG + tig0019233:176887-176906 Msa1371590:five_prime_UTR 35.0%
!! GTGATTTTAGAAAGGAGTTG+TGG - tig0019233:177434-177453 None:intergenic 35.0%
!! ATGCTTGGGTGATTTTAGAA+AGG - tig0019233:177442-177461 None:intergenic 35.0%
ATGAGAATGATTGGAAGTGA+AGG - tig0019233:177467-177486 None:intergenic 35.0%
TTCACTTCCAATCATTCTCA+TGG + tig0019233:177466-177485 Msa1371590:CDS 35.0%
CAAGTTGTAATGGCTAGATT+TGG + tig0019233:177671-177690 Msa1371590:CDS 35.0%
! TGATTTGAGTTCGCTTCATA+CGG + tig0019233:177931-177950 Msa1371590:CDS 35.0%
TCCGTTACTTCTTTACTCAA+AGG - tig0019233:177974-177993 None:intergenic 35.0%
! TTGAGTAAAGAAGTAACGGA+AGG + tig0019233:177974-177993 Msa1371590:CDS 35.0%
! TGAGTAAAGAAGTAACGGAA+GGG + tig0019233:177975-177994 Msa1371590:CDS 35.0%
TATGGTTTAACTGAATCCTC+TGG + tig0019233:178037-178056 Msa1371590:CDS 35.0%
!! TTAACTGAATCCTCTGGAAT+TGG + tig0019233:178043-178062 Msa1371590:CDS 35.0%
AACGAACCTTTCATAATGGT+GGG - tig0019233:178211-178230 None:intergenic 35.0%
AAACGAACCTTTCATAATGG+TGG - tig0019233:178212-178231 None:intergenic 35.0%
GAATTGCTAAACAAGTTTCC+GGG - tig0019233:178408-178427 None:intergenic 35.0%
TTGATGAGAATGAGAACATG+TGG + tig0019233:178457-178476 Msa1371590:intron 35.0%
AATGAGAACATGTGGTCTAT+TGG + tig0019233:178465-178484 Msa1371590:intron 35.0%
AAAGTTTGAGAGGGTAAAGT+TGG - tig0019233:178597-178616 None:intergenic 35.0%
TGTGTGAGAAAAGTTTGAGA+GGG - tig0019233:178606-178625 None:intergenic 35.0%
TTGTGTGAGAAAAGTTTGAG+AGG - tig0019233:178607-178626 None:intergenic 35.0%
TTGGCACACAGATTAAGAAA+TGG - tig0019233:178714-178733 None:intergenic 35.0%
!! TTCAAAATGAGTGTCGCTTT+AGG - tig0019233:178739-178758 None:intergenic 35.0%
!!! GACACTCATTTTGAAACAGA+GGG + tig0019233:178744-178763 Msa1371590:intron 35.0%
ACTCACACAAATGGATATTG+GGG + tig0019233:178855-178874 Msa1371590:intron 35.0%
TAGATGCAAAGGAAAAAGCT+TGG + tig0019233:179071-179090 Msa1371590:intron 35.0%
TAATACCAGCAACTAACCAA+AGG - tig0019233:179101-179120 None:intergenic 35.0%
!! GTTTGCTATATTGATAGCGA+TGG + tig0019233:179262-179281 Msa1371590:CDS 35.0%
GAAAGAGCTCATTAAGTACA+AGG + tig0019233:179309-179328 Msa1371590:CDS 35.0%
CATTAAGTACAAGGGATATC+AGG + tig0019233:179318-179337 Msa1371590:CDS 35.0%
TATCAGGTATCCAAATAAGG+AGG + tig0019233:179739-179758 Msa1371590:intron 35.0%
CTGCAACAAAATCCATAACT+TGG - tig0019233:179823-179842 None:intergenic 35.0%
TCTATACCCAAAAATCCATC+TGG + tig0019233:180000-180019 Msa1371590:CDS 35.0%
! TTCCGAAGAATTTTACCAGA+TGG - tig0019233:180018-180037 None:intergenic 35.0%
ATCTGGTAAAATTCTTCGGA+AGG + tig0019233:180017-180036 Msa1371590:CDS 35.0%
TTATTCTCTATAAGGGAAGC+AGG - tig0019233:180201-180220 None:intergenic 35.0%
! AGAGTTGTTGGTAGGTGAAT+TGG - tig0019233:177193-177212 None:intergenic 40.0%
AATTCACCTACCAACAACTC+TGG + tig0019233:177192-177211 Msa1371590:CDS 40.0%
ACAGGGAAGTAAATGGAGTT+GGG - tig0019233:177293-177312 None:intergenic 40.0%
AACAGGGAAGTAAATGGAGT+TGG - tig0019233:177294-177313 None:intergenic 40.0%
ATTTCGCGAGATGTATTGAG+GGG - tig0019233:177365-177384 None:intergenic 40.0%
GATTTCGCGAGATGTATTGA+GGG - tig0019233:177366-177385 None:intergenic 40.0%
AGTGAAAGCAAGAACAGGTT+TGG - tig0019233:177408-177427 None:intergenic 40.0%
!! TGTTGTTGATGATGAGTTGC+TGG - tig0019233:177498-177517 None:intergenic 40.0%
GAAGATACTACTCCTTTGCT+TGG - tig0019233:177626-177645 None:intergenic 40.0%
CATCGCTATGAGATTCTTGT+GGG - tig0019233:177648-177667 None:intergenic 40.0%
!! AGCGATGGTTCAAGTTGTAA+TGG + tig0019233:177661-177680 Msa1371590:CDS 40.0%
TTCAGCATAGCAACCAGTAT+CGG - tig0019233:177884-177903 None:intergenic 40.0%
TTGGAAGAGAGTCGTAAGTA+TGG + tig0019233:178082-178101 Msa1371590:CDS 40.0%
AGAGAGTCGTAAGTATGGTA+CGG + tig0019233:178087-178106 Msa1371590:CDS 40.0%
GGAAAGACAAAACATCCAGA+TGG + tig0019233:178318-178337 Msa1371590:intron 40.0%
TATAAACTCGTGGATCCATC+TGG - tig0019233:178336-178355 None:intergenic 40.0%
!!! GACACTCATTTTGAAACGGA+TGG - tig0019233:178502-178521 None:intergenic 40.0%
!!! CGACACTCATTTTGAAACAG+AGG + tig0019233:178743-178762 Msa1371590:intron 40.0%
TGAAACAGAGGGAGTAATGT+TGG + tig0019233:178755-178774 Msa1371590:intron 40.0%
GTTGGTCATATGCTTGACTT+AGG + tig0019233:178773-178792 Msa1371590:intron 40.0%
! ACTGTTTGAGTTCGAACTTG+TGG + tig0019233:178919-178938 Msa1371590:intron 40.0%
! CTGTTTGAGTTCGAACTTGT+GGG + tig0019233:178920-178939 Msa1371590:intron 40.0%
AGTCATGCACGTCTAATGAA+TGG - tig0019233:179023-179042 None:intergenic 40.0%
! AGGTTATTTCAGTAACGAGG+AGG + tig0019233:179192-179211 Msa1371590:intron 40.0%
!! CAGAGGGTTGGTTAAAAACA+GGG + tig0019233:179236-179255 Msa1371590:CDS 40.0%
!! AGAGGGTTGGTTAAAAACAG+GGG + tig0019233:179237-179256 Msa1371590:CDS 40.0%
! CGATGGATTCTTATTTGTCG+TGG + tig0019233:179279-179298 Msa1371590:CDS 40.0%
TAGTTGAAGTGATCGAAACG+TGG - tig0019233:179386-179405 None:intergenic 40.0%
AGCGGTCTTGATTGATAAGA+AGG - tig0019233:179698-179717 None:intergenic 40.0%
TTCCAATGGCATATGTGGTA+AGG + tig0019233:179771-179790 Msa1371590:CDS 40.0%
TTCCTTACCACATATGCCAT+TGG - tig0019233:179776-179795 None:intergenic 40.0%
AATGGCATATGTGGTAAGGA+AGG + tig0019233:179775-179794 Msa1371590:CDS 40.0%
! TATGGATTTTGTTGCAGAGC+AGG + tig0019233:179826-179845 Msa1371590:CDS 40.0%
AGAGTGATCGATCCAAGAAG+TGG + tig0019233:177026-177045 Msa1371590:CDS 45.0%
GAGTGATCGATCCAAGAAGT+GGG + tig0019233:177027-177046 Msa1371590:CDS 45.0%
!! AAAGAGTGGTTTGGTGGAAG+TGG - tig0019233:177098-177117 None:intergenic 45.0%
! GCATCTAAAGAGTGGTTTGG+TGG - tig0019233:177104-177123 None:intergenic 45.0%
GTGGCATCTAAAGAGTGGTT+TGG - tig0019233:177107-177126 None:intergenic 45.0%
AAGTGGTGGCATCTAAAGAG+TGG - tig0019233:177112-177131 None:intergenic 45.0%
! GTTGTTGGTAGGTGAATTGG+TGG - tig0019233:177190-177209 None:intergenic 45.0%
ACTTCCTCACTCTCCAACAT+GGG + tig0019233:177233-177252 Msa1371590:CDS 45.0%
AGTAAATGGAGTTGGGAGAG+AGG - tig0019233:177286-177305 None:intergenic 45.0%
GCAGACAACAGGGAAGTAAA+TGG - tig0019233:177300-177319 None:intergenic 45.0%
AGATGTATTGAGGGGATTGG+TGG - tig0019233:177357-177376 None:intergenic 45.0%
CGATTTCGCGAGATGTATTG+AGG - tig0019233:177367-177386 None:intergenic 45.0%
GGAGTAGTGAAAGCAAGAAC+AGG - tig0019233:177413-177432 None:intergenic 45.0%
TGGAAGTGAAGGTGATGCTT+GGG - tig0019233:177456-177475 None:intergenic 45.0%
TTGGAAGTGAAGGTGATGCT+TGG - tig0019233:177457-177476 None:intergenic 45.0%
GTGGTGTCCATGAGAATGAT+TGG - tig0019233:177476-177495 None:intergenic 45.0%
!! TGTTGATGATGAGTTGCTGG+TGG - tig0019233:177495-177514 None:intergenic 45.0%
TCAGTCACTCTACTCAACTC+AGG - tig0019233:177548-177567 None:intergenic 45.0%
TCCTGATGAGTAGAGTAGTG+TGG - tig0019233:177594-177613 None:intergenic 45.0%
CTCTACTCATCAGGAACAAC+TGG + tig0019233:177599-177618 Msa1371590:CDS 45.0%
CCATCGCTATGAGATTCTTG+TGG - tig0019233:177649-177668 None:intergenic 45.0%
CCACAAGAATCTCATAGCGA+TGG + tig0019233:177646-177665 Msa1371590:CDS 45.0%
!! GGTCTTGCAGTGTTTGCTAT+GGG + tig0019233:177752-177771 Msa1371590:CDS 45.0%
!! GTCTTGCAGTGTTTGCTATG+GGG + tig0019233:177753-177772 Msa1371590:CDS 45.0%
! TTTGAGTTCGCTTCATACGG+TGG + tig0019233:177934-177953 Msa1371590:CDS 45.0%
AGTAGAAGCTCCAATTCCAG+AGG - tig0019233:178056-178075 None:intergenic 45.0%
TGGAGCTTCTACTGAGTCTT+TGG + tig0019233:178063-178082 Msa1371590:CDS 45.0%
CTCAGCCGTTACCAGTTAAT+CGG + tig0019233:178161-178180 Msa1371590:CDS 45.0%
CCAGTCCGATTAACTGGTAA+CGG - tig0019233:178169-178188 None:intergenic 45.0%
!! TTAATCGGACTGGTGAGCTT+TGG + tig0019233:178176-178195 Msa1371590:CDS 45.0%
TATTGAACACGAGGTAGACC+CGG + tig0019233:178387-178406 Msa1371590:intron 45.0%
!! ATGAGTGTCGCTTTAGGCTT+TGG - tig0019233:178733-178752 None:intergenic 45.0%
! GAAAAAGCTTGGTGAGCCTT+TGG + tig0019233:179082-179101 Msa1371590:intron 45.0%
!! ATGCAAGACATGGTTGGTGA+TGG + tig0019233:179168-179187 Msa1371590:intron 45.0%
! GGCAGGTTATTTCAGTAACG+AGG + tig0019233:179189-179208 Msa1371590:intron 45.0%
ACATCGACCATTACTCCAGA+GGG + tig0019233:179220-179239 Msa1371590:CDS 45.0%
!! TAACCAACCCTCTGGAGTAA+TGG - tig0019233:179230-179249 None:intergenic 45.0%
!!! CCTGTTTTTAACCAACCCTC+TGG - tig0019233:179238-179257 None:intergenic 45.0%
!! CCAGAGGGTTGGTTAAAAAC+AGG + tig0019233:179235-179254 Msa1371590:CDS 45.0%
!! AACAAAGCCTCTAGTTCTGC+TGG - tig0019233:179532-179551 None:intergenic 45.0%
AGGTATCCAAATAAGGAGGC+TGG + tig0019233:179743-179762 Msa1371590:intron 45.0%
GGTATCCAAATAAGGAGGCT+GGG + tig0019233:179744-179763 Msa1371590:intron 45.0%
CTTGTCAGGAAGCCAAGTTA+TGG + tig0019233:179808-179827 Msa1371590:CDS 45.0%
! GCAGAGCAGGTTTGTTTGTT+TGG + tig0019233:179839-179858 Msa1371590:intron 45.0%
!! TATAATTAATTTATATTCTT+TGG - tig0019233:179603-179622 None:intergenic 5.0%
!!! ATTTATTTTTTATAATATGA+TGG + tig0019233:179655-179674 Msa1371590:intron 5.0%
!!! ATTAATTTTTTTCAATATAT+AGG + tig0019233:179936-179955 Msa1371590:intron 5.0%
CTGCTGCAAAACCCACTTCT+TGG - tig0019233:177041-177060 None:intergenic 50.0%
!! TGGTTTGGTGGAAGTGGAAG+GGG - tig0019233:177092-177111 None:intergenic 50.0%
!! GTGGTTTGGTGGAAGTGGAA+GGG - tig0019233:177093-177112 None:intergenic 50.0%
!! AGTGGTTTGGTGGAAGTGGA+AGG - tig0019233:177094-177113 None:intergenic 50.0%
AGGCGTCAATGAAGGCGATA+TGG - tig0019233:177160-177179 None:intergenic 50.0%
! TTGGTAGGTGAATTGGTGGC+CGG - tig0019233:177186-177205 None:intergenic 50.0%
GTTGGAGAGTGAGGAAGTGA+CGG - tig0019233:177231-177250 None:intergenic 50.0%
CACTTCCTCACTCTCCAACA+TGG + tig0019233:177232-177251 Msa1371590:CDS 50.0%
CTTCCTCACTCTCCAACATG+GGG + tig0019233:177234-177253 Msa1371590:CDS 50.0%
TCCAACATGGGGATCCGTAA+AGG + tig0019233:177245-177264 Msa1371590:CDS 50.0%
ACCTTTACGGATCCCCATGT+TGG - tig0019233:177249-177268 None:intergenic 50.0%
GGAGGATGACGTCACCTTTA+CGG - tig0019233:177262-177281 None:intergenic 50.0%
AAATGGAGTTGGGAGAGAGG+AGG - tig0019233:177283-177302 None:intergenic 50.0%
! TGTATTGAGGGGATTGGTGG+TGG - tig0019233:177354-177373 None:intergenic 50.0%
GCGAGATGTATTGAGGGGAT+TGG - tig0019233:177360-177379 None:intergenic 50.0%
GTAGTGTGGCTGTATCATCC+TGG - tig0019233:177580-177599 None:intergenic 50.0%
GCCACACTACTCTACTCATC+AGG + tig0019233:177590-177609 Msa1371590:CDS 50.0%
GGAACAACTGGACCAAGCAA+AGG + tig0019233:177611-177630 Msa1371590:CDS 50.0%
! CGGTCTTGCAGTGTTTGCTA+TGG + tig0019233:177751-177770 Msa1371590:CDS 50.0%
TGCTATGGGGCTTCTTGCTA+TGG + tig0019233:177766-177785 Msa1371590:CDS 50.0%
! GCTATGGGGCTTCTTGCTAT+GGG + tig0019233:177767-177786 Msa1371590:CDS 50.0%
! AGCATAGCAACCAGTATCGG+CGG - tig0019233:177881-177900 None:intergenic 50.0%
TCGCTTCATACGGTGGTTTC+TGG + tig0019233:177941-177960 Msa1371590:CDS 50.0%
! CTTCATACGGTGGTTTCTGG+TGG + tig0019233:177944-177963 Msa1371590:CDS 50.0%
GAGTCGTAAGTATGGTACGG+CGG + tig0019233:178090-178109 Msa1371590:CDS 50.0%
AGTCGTAAGTATGGTACGGC+GGG + tig0019233:178091-178110 Msa1371590:CDS 50.0%
GTCAACAATCATAGCCTCCG+TGG - tig0019233:178131-178150 None:intergenic 50.0%
CCGTTACCAGTTAATCGGAC+TGG + tig0019233:178166-178185 Msa1371590:CDS 50.0%
AGCTCACCAGTCCGATTAAC+TGG - tig0019233:178175-178194 None:intergenic 50.0%
CGTGGTCCCACCATTATGAA+AGG + tig0019233:178202-178221 Msa1371590:CDS 50.0%
GGATATTGGGGATCGAATCC+TGG + tig0019233:178867-178886 Msa1371590:intron 50.0%
ATTGTTAGGCAGAGCACACC+AGG - tig0019233:178888-178907 None:intergenic 50.0%
TAGACGTGCATGACTCTCCA+AGG + tig0019233:179028-179047 Msa1371590:intron 50.0%
! GTGAGCCTTTGGTTAGTTGC+TGG + tig0019233:179093-179112 Msa1371590:intron 50.0%
!! AAGACATGGTTGGTGATGGC+AGG + tig0019233:179172-179191 Msa1371590:intron 50.0%
!! CTCTGGAGTAATGGTCGATG+TGG - tig0019233:179221-179240 None:intergenic 50.0%
CACATCGACCATTACTCCAG+AGG + tig0019233:179219-179238 Msa1371590:CDS 50.0%
!! AAGCCTCTAGTTCTGCTGGA+GGG - tig0019233:179528-179547 None:intergenic 50.0%
!! AAAGCCTCTAGTTCTGCTGG+AGG - tig0019233:179529-179548 None:intergenic 50.0%
ACAGCAGCGTCTACAATAGC+AGG - tig0019233:179565-179584 None:intergenic 50.0%
GCAGGTTCCAATGGCATATG+TGG + tig0019233:179766-179785 Msa1371590:CDS 50.0%
GCATATGTGGTAAGGAAGGC+TGG + tig0019233:179779-179798 Msa1371590:CDS 50.0%
! AAGGCTGGAAGTAGCTTGTC+AGG + tig0019233:179794-179813 Msa1371590:CDS 50.0%
! GAAGCATTCAGGAATGCAGC+AGG - tig0019233:180179-180198 None:intergenic 50.0%
!! AAGGGAAGCAGGAAGCATTC+AGG - tig0019233:180190-180209 None:intergenic 50.0%
AGCACGCCAGAGTTGTTGGT+AGG - tig0019233:177201-177220 None:intergenic 55.0%
CTACAGCACGCCAGAGTTGT+TGG - tig0019233:177205-177224 None:intergenic 55.0%
GATCCCCATGTTGGAGAGTG+AGG - tig0019233:177240-177259 None:intergenic 55.0%
TGACGGAGAGGCAGACAACA+GGG - tig0019233:177310-177329 None:intergenic 55.0%
! ATGACGGAGAGGCAGACAAC+AGG - tig0019233:177311-177330 None:intergenic 55.0%
AGTGACTGAGCGCATCAACC+AGG + tig0019233:177559-177578 Msa1371590:CDS 55.0%
GTCGTAAGTATGGTACGGCG+GGG + tig0019233:178092-178111 Msa1371590:CDS 55.0%
! ACTGGTGAGCTTTGGCTTCG+TGG + tig0019233:178184-178203 Msa1371590:CDS 55.0%
CGACCATTACTCCAGAGGGT+TGG + tig0019233:179224-179243 Msa1371590:CDS 55.0%
TCCAAATAAGGAGGCTGGGC+AGG + tig0019233:179748-179767 Msa1371590:CDS 55.0%
ACCTGCCCAGCCTCCTTATT+TGG - tig0019233:179752-179771 None:intergenic 55.0%
GCGGGAGGAGATGAAAGTGG+TGG - tig0019233:177126-177145 None:intergenic 60.0%
AGCGCGGGAGGAGATGAAAG+TGG - tig0019233:177129-177148 None:intergenic 60.0%
ATCTCCTCCCGCGCTCATCA+CGG + tig0019233:177134-177153 Msa1371590:CDS 60.0%
GATATGGCCGTGATGAGCGC+GGG - tig0019233:177144-177163 None:intergenic 60.0%
CGATATGGCCGTGATGAGCG+CGG - tig0019233:177145-177164 None:intergenic 60.0%
GCCTTCATTGACGCCTCCAC+CGG + tig0019233:177164-177183 Msa1371590:CDS 60.0%
! GTAGGTGAATTGGTGGCCGG+TGG - tig0019233:177183-177202 None:intergenic 60.0%
TGGAGTTGGGAGAGAGGAGG+AGG - tig0019233:177280-177299 None:intergenic 60.0%
TGCCTCTCCGTCATGTCCCT+CGG + tig0019233:177317-177336 Msa1371590:CDS 60.0%
! GATAGCGCCGAGGGACATGA+CGG - tig0019233:177327-177346 None:intergenic 60.0%
! GGTGGTGATGATAGCGCCGA+GGG - tig0019233:177336-177355 None:intergenic 60.0%
! TGGTGGTGATGATAGCGCCG+AGG - tig0019233:177337-177356 None:intergenic 60.0%
CGGCGGCACAAGTGGTAAGA+AGG - tig0019233:177864-177883 None:intergenic 60.0%
ACCACTTGTGCCGCCGATAC+TGG + tig0019233:177868-177887 Msa1371590:CDS 60.0%
!! ACCAGTATCGGCGGCACAAG+TGG - tig0019233:177872-177891 None:intergenic 60.0%
GGGGCTTGTGTCTGCTTCCA+CGG + tig0019233:178111-178130 Msa1371590:CDS 60.0%
GCTTGTGTCTGCTTCCACGG+AGG + tig0019233:178114-178133 Msa1371590:CDS 60.0%
GGTCCCTCCAGCAGAACTAG+AGG + tig0019233:179522-179541 Msa1371590:intron 60.0%
!! GCCGGTGGAGGCGTCAATGA+AGG - tig0019233:177168-177187 None:intergenic 65.0%
! GGTGAATTGGTGGCCGGTGG+AGG - tig0019233:177180-177199 None:intergenic 65.0%
GGAGGCTGGGCAGGTTCCAA+TGG + tig0019233:179757-179776 Msa1371590:CDS 65.0%
ATGGCCGTGATGAGCGCGGG+AGG - tig0019233:177141-177160 None:intergenic 70.0%
! CGCCGAGGGACATGACGGAG+AGG - tig0019233:177322-177341 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0019233 gene 176830 180512 176830 ID=Msa1371590;Name=Msa1371590
tig0019233 mRNA 176830 180512 176830 ID=Msa1371590-mRNA-1;Parent=Msa1371590;Name=Msa1371590-mRNA-1;_AED=0.05;_eAED=0.05;_QI=163|1|1|1|1|1|5|441|553
tig0019233 exon 176830 178223 176830 ID=Msa1371590-mRNA-1:exon:2291;Parent=Msa1371590-mRNA-1
tig0019233 exon 179194 179339 179194 ID=Msa1371590-mRNA-1:exon:2292;Parent=Msa1371590-mRNA-1
tig0019233 exon 179523 179590 179523 ID=Msa1371590-mRNA-1:exon:2293;Parent=Msa1371590-mRNA-1
tig0019233 exon 179745 179847 179745 ID=Msa1371590-mRNA-1:exon:2294;Parent=Msa1371590-mRNA-1
tig0019233 exon 179958 180512 179958 ID=Msa1371590-mRNA-1:exon:2295;Parent=Msa1371590-mRNA-1
tig0019233 five_prime_UTR 176830 176992 176830 ID=Msa1371590-mRNA-1:five_prime_utr;Parent=Msa1371590-mRNA-1
tig0019233 CDS 176993 178223 176993 ID=Msa1371590-mRNA-1:cds;Parent=Msa1371590-mRNA-1
tig0019233 CDS 179194 179339 179194 ID=Msa1371590-mRNA-1:cds;Parent=Msa1371590-mRNA-1
tig0019233 CDS 179523 179590 179523 ID=Msa1371590-mRNA-1:cds;Parent=Msa1371590-mRNA-1
tig0019233 CDS 179745 179847 179745 ID=Msa1371590-mRNA-1:cds;Parent=Msa1371590-mRNA-1
tig0019233 CDS 179958 180071 179958 ID=Msa1371590-mRNA-1:cds;Parent=Msa1371590-mRNA-1
tig0019233 three_prime_UTR 180072 180512 180072 ID=Msa1371590-mRNA-1:three_prime_utr;Parent=Msa1371590-mRNA-1
Gene Sequence

>Msa1371590

ATGGAGGTAAACGGTGAGAATACGAATAGCAGAAGAGTGATCGATCCAAGAAGTGGGTTTTGCAGCAGCAATTCAATCTTCTACAGCAAGAGAAAACCCCTTCCACTTCCACCAAACCACTCTTTAGATGCCACCACTTTCATCTCCTCCCGCGCTCATCACGGCCATATCGCCTTCATTGACGCCTCCACCGGCCACCAATTCACCTACCAACAACTCTGGCGTGCTGTAGACTCCGTCACTTCCTCACTCTCCAACATGGGGATCCGTAAAGGTGACGTCATCCTCCTCCTCTCTCCCAACTCCATTTACTTCCCTGTTGTCTGCCTCTCCGTCATGTCCCTCGGCGCTATCATCACCACCACCAATCCCCTCAATACATCTCGCGAAATCGCTAAACAGATCGCTGATTCCAAACCTGTTCTTGCTTTCACTACTCCACAACTCCTTTCTAAAATCACCCAAGCATCACCTTCACTTCCAATCATTCTCATGGACACCACCAGCAACTCATCATCAACAACAACACTTGATGAAATGATGAAGAAAGAACCTGAGTTGAGTAGAGTGACTGAGCGCATCAACCAGGATGATACAGCCACACTACTCTACTCATCAGGAACAACTGGACCAAGCAAAGGAGTAGTATCTTCCCACAAGAATCTCATAGCGATGGTTCAAGTTGTAATGGCTAGATTTGGTAAAGAACAAGAGACGAGAGAAACTTTCATATGCACTGTTCCGATGTTTCATATATACGGTCTTGCAGTGTTTGCTATGGGGCTTCTTGCTATGGGTTCAACCATAGTTGTTCTCTCTAAATTCGAGATGCATGACATGCTTTCATCTATTGAGAAATTCAGAGCAACCTTCTTACCACTTGTGCCGCCGATACTGGTTGCTATGCTGAACAACGCAGATGCGATTAAGAGAAAGTATGATTTGAGTTCGCTTCATACGGTGGTTTCTGGTGGTGCTCCTTTGAGTAAAGAAGTAACGGAAGGGTTTATAGAAAAGTATCCTAATGTAAGTATCTTTCAAGGTTATGGTTTAACTGAATCCTCTGGAATTGGAGCTTCTACTGAGTCTTTGGAAGAGAGTCGTAAGTATGGTACGGCGGGGCTTGTGTCTGCTTCCACGGAGGCTATGATTGTTGACACTGAGACTGCTCAGCCGTTACCAGTTAATCGGACTGGTGAGCTTTGGCTTCGTGGTCCCACCATTATGAAAGGTTATTTCAGTAACGAGGAGGCAACCACATCGACCATTACTCCAGAGGGTTGGTTAAAAACAGGGGATGTTTGCTATATTGATAGCGATGGATTCTTATTTGTCGTGGATCGCTTGAAAGAGCTCATTAAGTACAAGGGATATCAGGTCCCTCCAGCAGAACTAGAGGCTTTGTTGCTGACTCATCCTGCTATTGTAGACGCTGCTGTCATACCGTATCCAAATAAGGAGGCTGGGCAGGTTCCAATGGCATATGTGGTAAGGAAGGCTGGAAGTAGCTTGTCAGGAAGCCAAGTTATGGATTTTGTTGCAGAGCAGGTGGCTCCATACAAAAGAATTCGAAAAGTGGCATTTATATCTTCTATACCCAAAAATCCATCTGGTAAAATTCTTCGGAAGGATCTTATCAAGCTAGCTACGTCTAAACTTTGA
Protein sequence

>Msa1371590

MEVNGENTNSRRVIDPRSGFCSSNSIFYSKRKPLPLPPNHSLDATTFISSRAHHGHIAFIDASTGHQFTYQQLWRAVDSVTSSLSNMGIRKGDVILLLSPNSIYFPVVCLSVMSLGAIITTTNPLNTSREIAKQIADSKPVLAFTTPQLLSKITQASPSLPIILMDTTSNSSSTTTLDEMMKKEPELSRVTERINQDDTATLLYSSGTTGPSKGVVSSHKNLIAMVQVVMARFGKEQETRETFICTVPMFHIYGLAVFAMGLLAMGSTIVVLSKFEMHDMLSSIEKFRATFLPLVPPILVAMLNNADAIKRKYDLSSLHTVVSGGAPLSKEVTEGFIEKYPNVSIFQGYGLTESSGIGASTESLEESRKYGTAGLVSASTEAMIVDTETAQPLPVNRTGELWLRGPTIMKGYFSNEEATTSTITPEGWLKTGDVCYIDSDGFLFVVDRLKELIKYKGYQVPPAELEALLLTHPAIVDAAVIPYPNKEAGQVPMAYVVRKAGSSLSGSQVMDFVAEQVAPYKRIRKVAFISSIPKNPSGKILRKDLIKLATSKL*