AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1379700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1379700 MtrunA17_Chr1g0157401 95.536 560 18 1 1 553 1 560 0.0 1098
Msa1379700 MtrunA17_Chr8g0353341 80.189 530 87 5 1 516 1 526 0.0 858
Msa1379700 MtrunA17_Chr1g0157431 94.000 350 14 1 1 343 1 350 0.0 675
Msa1379700 MtrunA17_Chr4g0028411 45.672 543 286 5 17 553 3 542 4.24e-162 472
Msa1379700 MtrunA17_Chr4g0039791 44.624 558 297 5 4 553 8 561 4.21e-161 470
Msa1379700 MtrunA17_Chr3g0116271 45.307 554 270 11 14 549 16 554 6.77e-160 467
Msa1379700 MtrunA17_Chr5g0409771 43.750 560 303 6 1 553 1 555 8.97e-159 464
Msa1379700 MtrunA17_Chr1g0197391 46.875 544 271 8 15 546 22 559 3.36e-158 463
Msa1379700 MtrunA17_Chr3g0116251 44.464 560 277 12 7 549 10 552 2.68e-157 461
Msa1379700 MtrunA17_Chr2g0327341 43.693 547 288 6 17 553 3 539 3.37e-153 449
Msa1379700 MtrunA17_Chr5g0409791 44.973 547 277 9 26 553 27 568 2.62e-152 448
Msa1379700 MtrunA17_Chr1g0180541 43.808 541 288 8 27 553 13 551 5.48e-145 429
Msa1379700 MtrunA17_Chr1g0180551 43.486 545 288 8 27 553 82 624 1.17e-142 425
Msa1379700 MtrunA17_Chr1g0186071 43.796 548 283 10 8 547 20 550 2.32e-139 415
Msa1379700 MtrunA17_Chr4g0072561 38.593 526 315 4 26 545 17 540 5.58e-118 360
Msa1379700 MtrunA17_Chr3g0102411 35.261 536 323 7 20 543 8 531 1.58e-116 356
Msa1379700 MtrunA17_Chr5g0395431 39.212 533 303 8 26 545 55 579 2.71e-116 356
Msa1379700 MtrunA17_Chr4g0000461 40.341 528 303 8 26 545 11 534 5.28e-114 349
Msa1379700 MtrunA17_Chr2g0334131 50.357 280 129 4 272 545 1 276 2.34e-86 269
Msa1379700 MtrunA17_Chr6g0471921 28.880 509 335 13 44 543 15 505 5.33e-52 185
Msa1379700 MtrunA17_Chr8g0370701 27.843 510 317 15 67 542 81 573 6.65e-47 173
Msa1379700 MtrunA17_Chr3g0109731 27.308 520 329 13 65 545 37 546 7.54e-46 169
Msa1379700 MtrunA17_Chr5g0396311 27.621 496 326 12 68 539 40 526 7.80e-44 163
Msa1379700 MtrunA17_Chr5g0433241 26.705 528 318 14 68 549 40 544 2.19e-41 157
Msa1379700 MtrunA17_Chr5g0396331 25.605 496 334 13 68 539 40 524 5.61e-39 150
Msa1379700 MtrunA17_Chr8g0370731 27.101 476 302 15 98 542 23 484 2.54e-37 144
Msa1379700 MtrunA17_Chr2g0301671 24.723 542 359 17 49 553 29 558 1.02e-36 144
Msa1379700 MtrunA17_Chr5g0433231 25.528 521 334 11 68 549 40 545 1.54e-36 143
Msa1379700 MtrunA17_Chr5g0433221 25.434 519 337 11 68 549 40 545 1.77e-36 143
Msa1379700 MtrunA17_Chr4g0068151 24.670 531 347 14 67 553 45 566 4.29e-35 139
Msa1379700 MtrunA17_Chr6g0471311 26.232 568 355 16 24 549 2 547 1.11e-34 137
Msa1379700 MtrunA17_Chr7g0252691 23.092 524 363 11 68 553 206 727 1.00e-33 136
Msa1379700 MtrunA17_Chr5g0406681 24.803 508 334 16 67 542 67 558 7.00e-32 129
Msa1379700 MtrunA17_Chr8g0377991 25.919 571 301 18 69 528 110 669 1.02e-29 124
Msa1379700 MtrunA17_Chr3g0121951 26.177 531 274 18 69 491 106 626 1.80e-28 120
Msa1379700 MtrunA17_Chr4g0028451 52.336 107 42 1 411 517 10 107 6.93e-28 107
Msa1379700 MtrunA17_Chr2g0292471 24.612 451 302 12 69 485 29 475 1.43e-25 110
Msa1379700 MtrunA17_Chr4g0004841 29.839 248 157 7 317 552 97 339 1.96e-25 107
Msa1379700 MtrunA17_Chr4g0004101 24.390 328 215 8 251 552 10 330 2.30e-24 104
Msa1379700 MtrunA17_Chr1g0205711 25.109 458 256 16 62 466 141 564 1.32e-21 99.0
Msa1379700 MtrunA17_Chr1g0160041 28.235 340 176 13 198 475 250 583 1.82e-19 92.4
Msa1379700 MtrunA17_Chr4g0033141 25.734 443 268 17 158 552 643 1072 3.71e-19 92.0
Msa1379700 MtrunA17_Chr2g0329161 24.943 441 266 17 158 547 638 1064 8.04e-19 90.9
Msa1379700 MtrunA17_Chr8g0388081 24.850 334 187 12 196 475 160 483 2.37e-18 88.6
Msa1379700 MtrunA17_Chr1g0194371 23.340 527 309 20 35 480 36 548 2.92e-18 88.6
Msa1379700 MtrunA17_Chr3g0121931 33.503 197 114 5 322 505 454 646 2.46e-16 82.4
Msa1379700 MtrunA17_Chr2g0334121 29.442 197 133 3 26 216 12 208 1.84e-15 75.5
Msa1379700 MtrunA17_Chr7g0270221 34.756 164 99 6 321 480 349 508 4.62e-15 78.2
Msa1379700 MtrunA17_Chr4g0034181 22.626 495 295 19 62 485 72 549 1.94e-14 76.3
Msa1379700 MtrunA17_Chr3g0124631 33.735 166 93 7 321 475 426 585 8.11e-14 74.3
Msa1379700 MtrunA17_Chr5g0396691 23.030 330 198 10 196 475 221 544 9.04e-14 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1379700 AT1G20510.1 74.630 540 134 2 14 551 4 542 0.0 828
Msa1379700 AT1G20500.1 66.302 549 170 5 13 553 9 550 0.0 738
Msa1379700 AT1G20510.2 73.673 471 121 2 14 482 4 473 0.0 711
Msa1379700 AT1G20480.1 61.429 560 202 4 4 553 10 565 0.0 708
Msa1379700 AT5G38120.1 61.580 557 193 7 9 553 3 550 0.0 693
Msa1379700 AT1G20510.3 73.051 449 118 2 14 460 4 451 0.0 675
Msa1379700 AT5G38120.3 60.269 521 186 7 9 517 3 514 0.0 635
Msa1379700 AT5G38120.4 60.494 486 171 7 9 482 3 479 0.0 590
Msa1379700 AT5G38120.2 60.494 486 171 7 9 482 3 479 0.0 590
Msa1379700 AT1G20490.2 60.047 428 154 5 13 430 9 429 1.92e-179 513
Msa1379700 AT1G20490.1 60.047 428 154 5 13 430 9 429 1.92e-179 513
Msa1379700 AT5G63380.1 43.162 563 286 8 8 549 5 554 2.85e-165 481
Msa1379700 AT4G19010.1 41.230 553 301 6 15 553 20 562 6.06e-156 457
Msa1379700 AT4G05160.1 44.059 547 291 7 17 553 3 544 1.33e-155 456
Msa1379700 AT3G21240.1 39.662 532 312 6 21 545 17 546 3.63e-121 368
Msa1379700 AT1G65060.1 40.832 529 298 6 26 545 34 556 1.98e-120 367
Msa1379700 AT1G51680.1 39.560 546 316 6 12 545 10 553 8.54e-113 347
Msa1379700 AT3G21230.1 38.817 541 310 9 22 545 24 560 4.39e-111 343
Msa1379700 AT1G62940.1 35.810 525 328 4 26 545 12 532 7.17e-103 320
Msa1379700 AT3G21230.3 37.308 520 301 10 22 524 24 535 6.87e-98 308
Msa1379700 AT1G51680.3 37.645 518 309 6 12 517 10 525 8.02e-98 307
Msa1379700 AT3G21240.2 37.712 472 285 6 21 485 17 486 1.60e-96 302
Msa1379700 AT3G21240.3 37.712 472 285 6 21 485 17 486 1.60e-96 302
Msa1379700 AT3G21230.2 37.317 477 278 9 22 481 24 496 8.15e-89 283
Msa1379700 AT1G51680.2 37.344 482 288 6 12 481 10 489 3.85e-88 281
Msa1379700 AT1G65060.2 37.444 446 264 6 26 462 34 473 1.00e-81 264
Msa1379700 AT3G21230.4 32.924 407 252 9 22 411 24 426 3.13e-52 184
Msa1379700 AT3G48990.1 28.008 482 335 10 67 543 30 504 1.44e-47 174
Msa1379700 AT1G65890.1 28.060 531 317 18 67 549 39 552 1.23e-46 172
Msa1379700 AT1G21530.1 27.514 527 323 15 62 549 37 543 3.07e-46 171
Msa1379700 AT1G20560.1 27.188 537 348 14 34 542 14 535 5.41e-46 170
Msa1379700 AT1G21530.2 27.238 525 329 14 62 549 37 545 1.02e-45 169
Msa1379700 AT1G77240.1 26.515 528 338 15 58 551 32 543 3.14e-45 167
Msa1379700 AT1G65880.1 28.839 534 310 20 67 549 39 553 3.90e-45 168
Msa1379700 AT1G21540.1 27.746 519 323 14 69 551 46 548 1.89e-44 166
Msa1379700 AT1G76290.1 28.101 516 318 18 67 549 39 534 2.45e-44 165
Msa1379700 AT1G66120.1 27.810 525 323 15 67 551 39 547 6.03e-44 164
Msa1379700 AT5G16370.1 26.628 522 326 14 74 553 43 549 2.01e-41 157
Msa1379700 AT2G17650.1 27.321 560 343 20 37 549 55 597 3.21e-40 154
Msa1379700 AT5G16340.1 26.796 515 321 14 74 547 43 542 1.64e-39 152
Msa1379700 AT1G20560.2 27.692 455 292 12 116 542 12 457 3.46e-39 149
Msa1379700 AT1G30520.2 27.155 464 292 11 64 496 34 482 8.14e-38 147
Msa1379700 AT1G30520.1 27.155 464 292 11 64 496 34 482 8.14e-38 147
Msa1379700 AT1G75960.1 26.488 521 324 14 68 549 41 541 8.88e-37 144
Msa1379700 AT1G68270.1 24.952 521 308 16 67 545 39 518 2.44e-33 134
Msa1379700 AT3G16170.1 24.672 381 245 9 197 547 234 602 3.27e-32 131
Msa1379700 AT5G36880.6 22.761 536 362 13 60 553 144 669 3.33e-32 132
Msa1379700 AT5G36880.3 22.761 536 362 13 60 553 144 669 3.33e-32 132
Msa1379700 AT5G36880.5 22.761 536 362 13 60 553 144 669 3.33e-32 132
Msa1379700 AT5G36880.1 22.761 536 362 13 60 553 144 669 3.33e-32 132
Msa1379700 AT5G36880.2 22.948 536 361 14 60 553 194 719 4.57e-32 131
Msa1379700 AT3G16910.1 23.654 520 344 16 67 545 47 554 1.02e-31 129
Msa1379700 AT1G30520.3 25.843 445 281 12 64 477 34 460 1.51e-30 125
Msa1379700 AT1G30520.4 25.843 445 281 12 64 477 34 460 1.51e-30 125
Msa1379700 AT5G36880.4 21.957 460 312 11 60 481 144 594 3.63e-25 110
Msa1379700 AT5G27600.1 27.760 317 172 9 197 466 261 567 2.11e-22 102
Msa1379700 AT3G05970.1 28.707 317 169 9 197 466 261 567 5.36e-20 94.4
Msa1379700 AT1G64400.1 22.515 493 303 19 55 478 65 547 1.59e-19 92.8
Msa1379700 AT1G77590.2 27.874 348 167 14 198 475 100 433 2.91e-19 91.7
Msa1379700 AT1G77590.1 27.874 348 167 14 198 475 246 579 5.46e-19 91.3
Msa1379700 AT2G47240.4 26.571 350 200 12 180 480 147 488 6.43e-19 90.9
Msa1379700 AT2G47240.1 26.571 350 200 12 180 480 206 547 7.21e-19 90.9
Msa1379700 AT2G47240.2 26.571 350 200 12 180 480 206 547 7.21e-19 90.9
Msa1379700 AT2G47240.3 26.571 350 200 12 180 480 206 547 7.21e-19 90.9
Msa1379700 AT1G49430.1 25.000 344 199 13 193 485 219 554 1.02e-16 84.0
Msa1379700 AT4G11030.1 23.548 310 174 10 198 454 224 523 7.10e-15 78.2
Msa1379700 AT3G23790.1 32.632 190 103 6 322 490 455 640 4.15e-13 72.8
Msa1379700 AT3G23790.1 24.797 246 152 9 68 282 111 354 2.55e-12 70.1
Msa1379700 AT4G14070.1 29.600 250 145 8 322 546 471 714 5.64e-12 68.9

Find 179 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTGCCCAGCCTCCTTATT+TGG 0.136723 tig0020281:+95856 None:intergenic
TCGCTTCATACGGTGGTTTC+TGG 0.138183 tig0020281:-97664 Msa1379700:CDS
TGATAGGTTTCTATCGAATT+TGG 0.177748 tig0020281:-95514 Msa1379700:three_prime_UTR
CTGCTGCAAAACCCACTTCT+TGG 0.251921 tig0020281:+98567 None:intergenic
GTTGGAGTCAAAAGAGAAAA+AGG 0.268019 tig0020281:-98785 None:intergenic
ACCACTTGTGCCGCCGATAC+TGG 0.271545 tig0020281:-97737 Msa1379700:CDS
GTAAGTATCTTTCAAGGTTA+TGG 0.275288 tig0020281:-97586 Msa1379700:CDS
TGGAGCTTCTACTGAGTCTT+TGG 0.282200 tig0020281:-97542 Msa1379700:CDS
TTCTATCGAATTTGGTTAGA+TGG 0.317747 tig0020281:-95506 Msa1379700:three_prime_UTR
TTGTGTAGTATCTATCAAAC+TGG 0.323309 tig0020281:+98667 None:intergenic
TGTTGTTGATGATGAGTTGC+TGG 0.335506 tig0020281:+98110 None:intergenic
GTTCCGATGTTTCATATATA+CGG 0.342157 tig0020281:-97874 Msa1379700:CDS
TGCTATGGGGCTTCTTGCTA+TGG 0.342915 tig0020281:-97839 Msa1379700:CDS
TTAACTGAATCCTCTGGAAT+TGG 0.351281 tig0020281:-97562 Msa1379700:CDS
AGAATTGTCACATCACAAAT+TGG 0.354196 tig0020281:+95127 None:intergenic
AGCTCACCAGTCCGATTAAC+TGG 0.354985 tig0020281:+97433 None:intergenic
CTCTACTCATCAGGAACAAC+TGG 0.359656 tig0020281:-98006 Msa1379700:CDS
GCAGACAACAGGGAAGTAAA+TGG 0.362942 tig0020281:+98308 None:intergenic
TAGTTAATTAACTAAAGTTT+AGG 0.372221 tig0020281:-95157 Msa1379700:three_prime_UTR
CAAGTTGTAATGGCTAGATT+TGG 0.372468 tig0020281:-97934 Msa1379700:CDS
GGAGGATGACGTCACCTTTA+CGG 0.372564 tig0020281:+98346 None:intergenic
TTATATCAGGTATCCAAATA+AGG 0.373402 tig0020281:-95869 Msa1379700:intron
CATTAAGTACAAGGGATATC+AGG 0.379179 tig0020281:-96287 Msa1379700:intron
AAGGGAAGCAGGAAGCATTC+AGG 0.399635 tig0020281:+95418 None:intergenic
GGTGAATTGGTGGCCGGTGG+AGG 0.402061 tig0020281:+98428 None:intergenic
ATGACGGAGAGGCAGACAAC+AGG 0.402334 tig0020281:+98297 None:intergenic
AAGGCTGGAAGTAGCTTGTC+AGG 0.410549 tig0020281:-95811 Msa1379700:CDS
TTAATCGGACTGGTGAGCTT+TGG 0.414553 tig0020281:-97429 Msa1379700:CDS
TGGAGTTGGGAGAGAGGAGG+AGG 0.417129 tig0020281:+98328 None:intergenic
GAATTTAGAGAGAACAACTA+TGG 0.419281 tig0020281:+97810 None:intergenic
AAGAAACGAACCTTTCATAA+TGG 0.419398 tig0020281:+97393 None:intergenic
AGTAAATGGAGTTGGGAGAG+AGG 0.420482 tig0020281:+98322 None:intergenic
TTTGTGACAAAATAAAGAAA+TGG 0.421519 tig0020281:+95470 None:intergenic
TGGTTTGGTGGAAGTGGAAG+GGG 0.421746 tig0020281:+98516 None:intergenic
ACTGGTGAGCTTTGGCTTCG+TGG 0.421933 tig0020281:-97421 Msa1379700:CDS
AGTGAAAGCAAGAACAGGTT+TGG 0.423538 tig0020281:+98200 None:intergenic
ATCCATCTGGTAAAATTCTT+CGG 0.423771 tig0020281:-95592 Msa1379700:CDS
AAAGAGTGGTTTGGTGGAAG+TGG 0.424298 tig0020281:+98510 None:intergenic
CCTTGAAAGATACTTACATT+AGG 0.428705 tig0020281:+97592 None:intergenic
AGAGATTAAGATTAAGAAGA+TGG 0.429051 tig0020281:-98631 Msa1379700:exon
TCCAAATAAGGAGGCTGGGC+AGG 0.431867 tig0020281:-95857 Msa1379700:CDS
CCTAATGTAAGTATCTTTCA+AGG 0.432845 tig0020281:-97592 Msa1379700:CDS
CTCAGCCGTTACCAGTTAAT+CGG 0.436385 tig0020281:-97444 Msa1379700:CDS
GGTCTTGCAGTGTTTGCTAT+GGG 0.437424 tig0020281:-97853 Msa1379700:CDS
CGGTCTTGCAGTGTTTGCTA+TGG 0.438320 tig0020281:-97854 Msa1379700:CDS
GCTATGGGGCTTCTTGCTAT+GGG 0.440856 tig0020281:-97838 Msa1379700:CDS
CTACAGCACGCCAGAGTTGT+TGG 0.441513 tig0020281:+98403 None:intergenic
CTTGTCAGGAAGCCAAGTTA+TGG 0.458124 tig0020281:-95797 Msa1379700:CDS
TTGGAAGAGAGTCGTAAGTA+TGG 0.463115 tig0020281:-97523 Msa1379700:CDS
AGGCGTCAATGAAGGCGATA+TGG 0.463838 tig0020281:+98448 None:intergenic
GGGGCTTGTGTCTGCTTCCA+CGG 0.471317 tig0020281:-97494 Msa1379700:CDS
TATGGTTTAACTGAATCCTC+TGG 0.471540 tig0020281:-97568 Msa1379700:CDS
GTGGTTTGGTGGAAGTGGAA+GGG 0.473586 tig0020281:+98515 None:intergenic
AGAGTTGTTGGTAGGTGAAT+TGG 0.474858 tig0020281:+98415 None:intergenic
AGGTATCCAAATAAGGAGGC+TGG 0.481674 tig0020281:-95862 Msa1379700:CDS
TTCACTTCCAATCATTCTCA+TGG 0.482486 tig0020281:-98139 Msa1379700:CDS
TAACCAACCCTCTGGAGTAA+TGG 0.484888 tig0020281:+96378 None:intergenic
CGATTTCGCGAGATGTATTG+AGG 0.485277 tig0020281:+98241 None:intergenic
GCGAGATGTATTGAGGGGAT+TGG 0.486320 tig0020281:+98248 None:intergenic
AACAAAGCCTCTAGTTCTGC+TGG 0.486762 tig0020281:+96076 None:intergenic
CACTTCCTCACTCTCCAACA+TGG 0.488973 tig0020281:-98373 Msa1379700:CDS
GCCGGTGGAGGCGTCAATGA+AGG 0.490250 tig0020281:+98440 None:intergenic
AGTGGTTTGGTGGAAGTGGA+AGG 0.490859 tig0020281:+98514 None:intergenic
GCATATGTGGTAAGGAAGGC+TGG 0.491572 tig0020281:-95826 Msa1379700:CDS
TTATTCTCTATAAGGGAAGC+AGG 0.492146 tig0020281:+95407 None:intergenic
ATTAAGAAGATGGAGGTAAA+CGG 0.492454 tig0020281:-98621 Msa1379700:exon
TGTATTGAGGGGATTGGTGG+TGG 0.493354 tig0020281:+98254 None:intergenic
CGATGGATTCTTATTTGTCG+TGG 0.494381 tig0020281:-96326 Msa1379700:CDS
GTGGTGTCCATGAGAATGAT+TGG 0.496058 tig0020281:+98132 None:intergenic
AACAGGGAAGTAAATGGAGT+TGG 0.497843 tig0020281:+98314 None:intergenic
AAGGTAGTAAATAAATAATG+AGG 0.498509 tig0020281:-98766 Msa1379700:five_prime_UTR
CTGCAACAAAATCCATAACT+TGG 0.502133 tig0020281:+95785 None:intergenic
TGATTTGAGTTCGCTTCATA+CGG 0.505643 tig0020281:-97674 Msa1379700:CDS
TTGGTAGGTGAATTGGTGGC+CGG 0.506146 tig0020281:+98422 None:intergenic
GCCACACTACTCTACTCATC+AGG 0.506955 tig0020281:-98015 Msa1379700:CDS
AGTCGTAAGTATGGTACGGC+GGG 0.509532 tig0020281:-97514 Msa1379700:CDS
CGGCGGCACAAGTGGTAAGA+AGG 0.513016 tig0020281:+97744 None:intergenic
CATCGCTATGAGATTCTTGT+GGG 0.513470 tig0020281:+97960 None:intergenic
GCCTTCATTGACGCCTCCAC+CGG 0.515250 tig0020281:-98441 Msa1379700:CDS
GAGTGAATGAATGATGATAT+TGG 0.515321 tig0020281:-98725 Msa1379700:five_prime_UTR
TGGAAGTGAAGGTGATGCTT+GGG 0.520153 tig0020281:+98152 None:intergenic
GGAGTAGTGAAAGCAAGAAC+AGG 0.520555 tig0020281:+98195 None:intergenic
GAAGATACTACTCCTTTGCT+TGG 0.520592 tig0020281:+97982 None:intergenic
ACTTCCTCACTCTCCAACAT+GGG 0.520727 tig0020281:-98372 Msa1379700:CDS
TGCCTCTCCGTCATGTCCCT+CGG 0.521898 tig0020281:-98288 Msa1379700:CDS
TTCCTTACCACATATGCCAT+TGG 0.522529 tig0020281:+95832 None:intergenic
AGCGATGGTTCAAGTTGTAA+TGG 0.525827 tig0020281:-97944 Msa1379700:CDS
ACAGGGAAGTAAATGGAGTT+GGG 0.526332 tig0020281:+98315 None:intergenic
AGGTAGTAAATAAATAATGA+GGG 0.527522 tig0020281:-98765 Msa1379700:five_prime_UTR
TCCGTTACTTCTTTACTCAA+AGG 0.528090 tig0020281:+97634 None:intergenic
TTAACTAAAGTTTAGGCATG+AGG 0.532817 tig0020281:-95150 Msa1379700:three_prime_UTR
GTTTGCTATATTGATAGCGA+TGG 0.538596 tig0020281:-96343 Msa1379700:CDS
TTGGAAGTGAAGGTGATGCT+TGG 0.539417 tig0020281:+98151 None:intergenic
ATACAAAAGAATTCGAAAAG+TGG 0.543360 tig0020281:-95639 Msa1379700:CDS
TTCCAATGGCATATGTGGTA+AGG 0.544112 tig0020281:-95834 Msa1379700:CDS
CTTCATACGGTGGTTTCTGG+TGG 0.547015 tig0020281:-97661 Msa1379700:CDS
ATCTGGTAAAATTCTTCGGA+AGG 0.547613 tig0020281:-95588 Msa1379700:CDS
GGAGGCTGGGCAGGTTCCAA+TGG 0.547827 tig0020281:-95848 Msa1379700:CDS
ACAGCAGCGTCTACAATAGC+AGG 0.550475 tig0020281:+96043 None:intergenic
GTAGGTGAATTGGTGGCCGG+TGG 0.552038 tig0020281:+98425 None:intergenic
ACCTTTACGGATCCCCATGT+TGG 0.552224 tig0020281:+98359 None:intergenic
GAAAGAGCTCATTAAGTACA+AGG 0.552506 tig0020281:-96296 Msa1379700:CDS
GCATCTAAAGAGTGGTTTGG+TGG 0.553390 tig0020281:+98504 None:intergenic
CGTGGTCCCACCATTATGAA+AGG 0.554145 tig0020281:-97403 Msa1379700:intron
TCCAACATGGGGATCCGTAA+AGG 0.554642 tig0020281:-98360 Msa1379700:CDS
GCAGGTTCCAATGGCATATG+TGG 0.556198 tig0020281:-95839 Msa1379700:CDS
ACCAGTATCGGCGGCACAAG+TGG 0.558595 tig0020281:+97736 None:intergenic
AAACGAACCTTTCATAATGG+TGG 0.559397 tig0020281:+97396 None:intergenic
TTCAGCATAGCAACCAGTAT+CGG 0.561145 tig0020281:+97724 None:intergenic
CCAGTCCGATTAACTGGTAA+CGG 0.565679 tig0020281:+97439 None:intergenic
GGTATCCAAATAAGGAGGCT+GGG 0.569081 tig0020281:-95861 Msa1379700:CDS
CCATCGCTATGAGATTCTTG+TGG 0.569419 tig0020281:+97959 None:intergenic
GTGGCATCTAAAGAGTGGTT+TGG 0.570177 tig0020281:+98501 None:intergenic
CCGTTACCAGTTAATCGGAC+TGG 0.573347 tig0020281:-97439 Msa1379700:CDS
TTGAGTAAAGAAGTAACGGA+AGG 0.574145 tig0020281:-97631 Msa1379700:CDS
AGATGTATTGAGGGGATTGG+TGG 0.576476 tig0020281:+98251 None:intergenic
ATCTCCTCCCGCGCTCATCA+CGG 0.577531 tig0020281:-98471 Msa1379700:CDS
GATTAAGATTAAGAAGATGG+AGG 0.581479 tig0020281:-98628 Msa1379700:exon
GAAGCATTCAGGAATGCAGC+AGG 0.582823 tig0020281:+95429 None:intergenic
TCCTTTGAGTAAAGAAGTAA+CGG 0.584099 tig0020281:-97635 Msa1379700:CDS
GATTTCGCGAGATGTATTGA+GGG 0.584385 tig0020281:+98242 None:intergenic
AATTCACCTACCAACAACTC+TGG 0.585675 tig0020281:-98413 Msa1379700:CDS
AACGAACCTTTCATAATGGT+GGG 0.587290 tig0020281:+97397 None:intergenic
GATATGGCCGTGATGAGCGC+GGG 0.587698 tig0020281:+98464 None:intergenic
AAATGGAGTTGGGAGAGAGG+AGG 0.592217 tig0020281:+98325 None:intergenic
TGAGTAAAGAAGTAACGGAA+GGG 0.592240 tig0020281:-97630 Msa1379700:CDS
GTAGTAAATAAATAATGAGG+GGG 0.595227 tig0020281:-98763 Msa1379700:five_prime_UTR
GTTGTTGGTAGGTGAATTGG+TGG 0.596032 tig0020281:+98418 None:intergenic
AATGGCATATGTGGTAAGGA+AGG 0.598196 tig0020281:-95830 Msa1379700:CDS
GTCTTGCAGTGTTTGCTATG+GGG 0.598502 tig0020281:-97852 Msa1379700:CDS
CGATATGGCCGTGATGAGCG+CGG 0.598855 tig0020281:+98463 None:intergenic
TGACGGAGAGGCAGACAACA+GGG 0.598859 tig0020281:+98298 None:intergenic
GTTGGAGAGTGAGGAAGTGA+CGG 0.598886 tig0020281:+98377 None:intergenic
CGACCATTACTCCAGAGGGT+TGG 0.603546 tig0020281:-96381 Msa1379700:CDS
GATAGCGCCGAGGGACATGA+CGG 0.604148 tig0020281:+98281 None:intergenic
AGAGTGATCGATCCAAGAAG+TGG 0.604701 tig0020281:-98579 Msa1379700:CDS
AGTGACTGAGCGCATCAACC+AGG 0.608319 tig0020281:-98046 Msa1379700:CDS
TGTTGATGATGAGTTGCTGG+TGG 0.609037 tig0020281:+98113 None:intergenic
ATGGCCGTGATGAGCGCGGG+AGG 0.610511 tig0020281:+98467 None:intergenic
CCACAAGAATCTCATAGCGA+TGG 0.611191 tig0020281:-97959 Msa1379700:CDS
GGTCCCTCCAGCAGAACTAG+AGG 0.614140 tig0020281:-96083 Msa1379700:CDS
TTTGAGTTCGCTTCATACGG+TGG 0.616174 tig0020281:-97671 Msa1379700:CDS
GATCCCCATGTTGGAGAGTG+AGG 0.617341 tig0020281:+98368 None:intergenic
GGTGGTGATGATAGCGCCGA+GGG 0.619275 tig0020281:+98272 None:intergenic
AGAGAGTCGTAAGTATGGTA+CGG 0.623595 tig0020281:-97518 Msa1379700:CDS
AGCGCGGGAGGAGATGAAAG+TGG 0.628660 tig0020281:+98479 None:intergenic
GGTAGTAAATAAATAATGAG+GGG 0.629942 tig0020281:-98764 Msa1379700:five_prime_UTR
TGTGTAGTATCTATCAAACT+GGG 0.632023 tig0020281:+98668 None:intergenic
GGAACAACTGGACCAAGCAA+AGG 0.634924 tig0020281:-97994 Msa1379700:CDS
TCAGTCACTCTACTCAACTC+AGG 0.638010 tig0020281:+98060 None:intergenic
GTAGTGTGGCTGTATCATCC+TGG 0.639019 tig0020281:+98028 None:intergenic
ATGAGAATGATTGGAAGTGA+AGG 0.639263 tig0020281:+98141 None:intergenic
AAAGCCTCTAGTTCTGCTGG+AGG 0.643862 tig0020281:+96079 None:intergenic
AGCATAGCAACCAGTATCGG+CGG 0.644393 tig0020281:+97727 None:intergenic
GGCAGGTTATTTCAGTAACG+AGG 0.647450 tig0020281:-96416 Msa1379700:intron
ACATCGACCATTACTCCAGA+GGG 0.647619 tig0020281:-96385 Msa1379700:CDS
AAAGAGCTCATTAAGTACAA+GGG 0.651964 tig0020281:-96295 Msa1379700:CDS
GAGTGATCGATCCAAGAAGT+GGG 0.652473 tig0020281:-98578 Msa1379700:CDS
AGACCGTATATATGAAACAT+CGG 0.653796 tig0020281:+97871 None:intergenic
GCTTGTGTCTGCTTCCACGG+AGG 0.655096 tig0020281:-97491 Msa1379700:CDS
CTCTGGAGTAATGGTCGATG+TGG 0.656546 tig0020281:+96387 None:intergenic
AAGCCTCTAGTTCTGCTGGA+GGG 0.656701 tig0020281:+96080 None:intergenic
TAGTAAATAAATAATGAGGG+GGG 0.661298 tig0020281:-98762 Msa1379700:five_prime_UTR
ATTTCGCGAGATGTATTGAG+GGG 0.663874 tig0020281:+98243 None:intergenic
CTTCCTCACTCTCCAACATG+GGG 0.667378 tig0020281:-98371 Msa1379700:CDS
GCGGGAGGAGATGAAAGTGG+TGG 0.667975 tig0020281:+98482 None:intergenic
AGGTTATTTCAGTAACGAGG+AGG 0.670840 tig0020281:-96413 Msa1379700:CDS
CGCCGAGGGACATGACGGAG+AGG 0.671164 tig0020281:+98286 None:intergenic
AAGTGGTGGCATCTAAAGAG+TGG 0.673146 tig0020281:+98496 None:intergenic
TATCAGGTATCCAAATAAGG+AGG 0.676684 tig0020281:-95866 Msa1379700:intron
CACATCGACCATTACTCCAG+AGG 0.683319 tig0020281:-96386 Msa1379700:CDS
TCCTGATGAGTAGAGTAGTG+TGG 0.691172 tig0020281:+98014 None:intergenic
GTCGTAAGTATGGTACGGCG+GGG 0.691371 tig0020281:-97513 Msa1379700:CDS
AGCACGCCAGAGTTGTTGGT+AGG 0.696936 tig0020281:+98407 None:intergenic
AGTAGAAGCTCCAATTCCAG+AGG 0.697004 tig0020281:+97552 None:intergenic
TGGTGGTGATGATAGCGCCG+AGG 0.700534 tig0020281:+98271 None:intergenic
GTCAACAATCATAGCCTCCG+TGG 0.700669 tig0020281:+97477 None:intergenic
GAGTCGTAAGTATGGTACGG+CGG 0.704214 tig0020281:-97515 Msa1379700:CDS
TGAATGATGATATTGGACGA+CGG 0.752090 tig0020281:-98718 Msa1379700:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTAATTAATTAAAAA+AGG + tig0020281:96655-96674 None:intergenic 0.0%
!!! AAGCAAATATTATTTTTTTT+AGG - tig0020281:98580-98599 Msa1379700:CDS 10.0%
!!! AATATTATTTTTTTTAGGTT+TGG - tig0020281:98585-98604 Msa1379700:CDS 10.0%
!! GAAAAAAATTAAAGTTGTAT+TGG + tig0020281:96857-96876 None:intergenic 15.0%
!! ATTAATTTATATTCTTTGGT+AGG + tig0020281:97884-97903 None:intergenic 15.0%
!!! AATTTTTTTCAATATATAGG+TGG - tig0020281:98224-98243 Msa1379700:CDS 15.0%
!!! AACATTTTTATTCTCTATAA+GGG + tig0020281:98493-98512 None:intergenic 15.0%
!!! TCAATTTTAGCAATATTATA+CGG + tig0020281:98648-98667 None:intergenic 15.0%
!! TAGTTAATTAACTAAAGTTT+AGG - tig0020281:98733-98752 Msa1379700:five_prime_UTR 15.0%
!! AAGGTAGTAAATAAATAATG+AGG - tig0020281:95124-95143 Msa1379700:three_prime_UTR 20.0%
!! AGGTAGTAAATAAATAATGA+GGG - tig0020281:95125-95144 Msa1379700:three_prime_UTR 20.0%
!! ATAAACAATTCAGATTAGAT+TGG - tig0020281:96582-96601 Msa1379700:intron 20.0%
!! TAATTAAAATATTGAACACG+AGG - tig0020281:96663-96682 Msa1379700:intron 20.0%
!!! TGTCTTTTTCTAATAAAGTA+AGG - tig0020281:96933-96952 Msa1379700:intron 20.0%
!! AAATGGAATGAAATAATCAT+TGG + tig0020281:96982-97001 None:intergenic 20.0%
!!! ATCGATATTCTAAAAAATGT+GGG + tig0020281:97092-97111 None:intergenic 20.0%
!!! TATCGATATTCTAAAAAATG+TGG + tig0020281:97093-97112 None:intergenic 20.0%
!! AGTAAAATGTCAAAATTGTT+AGG + tig0020281:97187-97206 None:intergenic 20.0%
!!! GTTACTTTTTTTATGTCTAA+TGG + tig0020281:97286-97305 None:intergenic 20.0%
!!! TTTTGTGTGTATATTATATC+AGG - tig0020281:98008-98027 Msa1379700:CDS 20.0%
!! TTTGTGACAAAATAAAGAAA+TGG + tig0020281:98423-98442 None:intergenic 20.0%
!!! CAACATTTTTATTCTCTATA+AGG + tig0020281:98494-98513 None:intergenic 20.0%
!! TATAGAGAATAAAAATGTTG+TGG - tig0020281:98494-98513 Msa1379700:CDS 20.0%
! GGTAGTAAATAAATAATGAG+GGG - tig0020281:95126-95145 Msa1379700:three_prime_UTR 25.0%
! GTAGTAAATAAATAATGAGG+GGG - tig0020281:95127-95146 Msa1379700:three_prime_UTR 25.0%
! TAGTAAATAAATAATGAGGG+GGG - tig0020281:95128-95147 Msa1379700:three_prime_UTR 25.0%
! AGAGATTAAGATTAAGAAGA+TGG - tig0020281:95259-95278 Msa1379700:three_prime_UTR 25.0%
! AATTATAGCATATAAACTCG+TGG + tig0020281:96631-96650 None:intergenic 25.0%
! TTATTACAAACTCACACAAA+TGG - tig0020281:97131-97150 Msa1379700:intron 25.0%
!! TTTTAGTGTTATAAACACCT+TGG + tig0020281:97333-97352 None:intergenic 25.0%
! CACTAAAATAATAGATGCAA+AGG - tig0020281:97345-97364 Msa1379700:intron 25.0%
! ATATAAGCTCACATAATTGT+TGG - tig0020281:97407-97426 Msa1379700:CDS 25.0%
!!! CATAATTGTTGGTTCTATTA+TGG - tig0020281:97418-97437 Msa1379700:CDS 25.0%
! GTAAACAGTAATTTATCTCT+GGG + tig0020281:97699-97718 None:intergenic 25.0%
! TCTTTATTACTATTGTGTGT+CGG + tig0020281:97721-97740 None:intergenic 25.0%
!! TGTTTTATGGTTATTGTTAC+AGG - tig0020281:97786-97805 Msa1379700:CDS 25.0%
! TTATATCAGGTATCCAAATA+AGG - tig0020281:98021-98040 Msa1379700:CDS 25.0%
!!! ACTCTTTTTTATTAGATGCT+TGG - tig0020281:98166-98185 Msa1379700:CDS 25.0%
!!! CTCTTTTTTATTAGATGCTT+GGG - tig0020281:98167-98186 Msa1379700:CDS 25.0%
!! ACTTTTCGAATTCTTTTGTA+TGG + tig0020281:98252-98271 None:intergenic 25.0%
! ATACAAAAGAATTCGAAAAG+TGG - tig0020281:98251-98270 Msa1379700:CDS 25.0%
!!! ATTTTACCAGATGGATTTTT+GGG + tig0020281:98294-98313 None:intergenic 25.0%
!!! AATTTTACCAGATGGATTTT+TGG + tig0020281:98295-98314 None:intergenic 25.0%
!! CAGTTGAACTTTTAAATGAT+AGG - tig0020281:98360-98379 Msa1379700:CDS 25.0%
GAGTGAATGAATGATGATAT+TGG - tig0020281:95165-95184 Msa1379700:three_prime_UTR 30.0%
TGTGTAGTATCTATCAAACT+GGG + tig0020281:95225-95244 None:intergenic 30.0%
TTGTGTAGTATCTATCAAAC+TGG + tig0020281:95226-95245 None:intergenic 30.0%
GATTAAGATTAAGAAGATGG+AGG - tig0020281:95262-95281 Msa1379700:three_prime_UTR 30.0%
ATTAAGAAGATGGAGGTAAA+CGG - tig0020281:95269-95288 Msa1379700:three_prime_UTR 30.0%
GTTCCGATGTTTCATATATA+CGG - tig0020281:96016-96035 Msa1379700:intron 30.0%
AGACCGTATATATGAAACAT+CGG + tig0020281:96022-96041 None:intergenic 30.0%
GAATTTAGAGAGAACAACTA+TGG + tig0020281:96083-96102 None:intergenic 30.0%
TCCTTTGAGTAAAGAAGTAA+CGG - tig0020281:96255-96274 Msa1379700:intron 30.0%
CCTTGAAAGATACTTACATT+AGG + tig0020281:96301-96320 None:intergenic 30.0%
CCTAATGTAAGTATCTTTCA+AGG - tig0020281:96298-96317 Msa1379700:CDS 30.0%
GTAAGTATCTTTCAAGGTTA+TGG - tig0020281:96304-96323 Msa1379700:CDS 30.0%
AAGAAACGAACCTTTCATAA+TGG + tig0020281:96500-96519 None:intergenic 30.0%
TGAATTGCTAAACAAGTTTC+CGG + tig0020281:96694-96713 None:intergenic 30.0%
!! AAGTGACACTCATTTTGAAA+CGG + tig0020281:96791-96810 None:intergenic 30.0%
AAACTCACACAAATGGATAT+TGG - tig0020281:97138-97157 Msa1379700:intron 30.0%
AACTCACACAAATGGATATT+GGG - tig0020281:97139-97158 Msa1379700:intron 30.0%
GCTAATTAAAATGCAAGACA+TGG - tig0020281:97443-97462 Msa1379700:CDS 30.0%
ATTAAAATGCAAGACATGGT+TGG - tig0020281:97447-97466 Msa1379700:CDS 30.0%
AAAGAGCTCATTAAGTACAA+GGG - tig0020281:97595-97614 Msa1379700:CDS 30.0%
GGTAAACAGTAATTTATCTC+TGG + tig0020281:97700-97719 None:intergenic 30.0%
CGTAAATATTGTCAAACTCA+TGG - tig0020281:97748-97767 Msa1379700:CDS 30.0%
!!! GTTCTCTTGCTTTTGTTTTA+TGG - tig0020281:97773-97792 Msa1379700:CDS 30.0%
TATATTCTTTGGTAGGCTTA+CGG + tig0020281:97877-97896 None:intergenic 30.0%
TGATTGATAAGAAGGAATAC+TGG + tig0020281:97975-97994 None:intergenic 30.0%
TATACACACAAAACATTCAG+CGG + tig0020281:98001-98020 None:intergenic 30.0%
ATCCATCTGGTAAAATTCTT+CGG - tig0020281:98298-98317 Msa1379700:CDS 30.0%
TGATAGGTTTCTATCGAATT+TGG - tig0020281:98376-98395 Msa1379700:CDS 30.0%
TTCTATCGAATTTGGTTAGA+TGG - tig0020281:98384-98403 Msa1379700:CDS 30.0%
GAATAAAAATGTTGTGGTCT+TGG - tig0020281:98500-98519 Msa1379700:CDS 30.0%
TTAACTAAAGTTTAGGCATG+AGG - tig0020281:98740-98759 Msa1379700:five_prime_UTR 30.0%
AGAATTGTCACATCACAAAT+TGG + tig0020281:98766-98785 None:intergenic 30.0%
TGAATGATGATATTGGACGA+CGG - tig0020281:95172-95191 Msa1379700:three_prime_UTR 35.0%
!! GTGATTTTAGAAAGGAGTTG+TGG + tig0020281:95719-95738 None:intergenic 35.0%
!! ATGCTTGGGTGATTTTAGAA+AGG + tig0020281:95727-95746 None:intergenic 35.0%
ATGAGAATGATTGGAAGTGA+AGG + tig0020281:95752-95771 None:intergenic 35.0%
TTCACTTCCAATCATTCTCA+TGG - tig0020281:95751-95770 Msa1379700:intron 35.0%
CAAGTTGTAATGGCTAGATT+TGG - tig0020281:95956-95975 Msa1379700:intron 35.0%
! TGATTTGAGTTCGCTTCATA+CGG - tig0020281:96216-96235 Msa1379700:intron 35.0%
TCCGTTACTTCTTTACTCAA+AGG + tig0020281:96259-96278 None:intergenic 35.0%
! TTGAGTAAAGAAGTAACGGA+AGG - tig0020281:96259-96278 Msa1379700:intron 35.0%
! TGAGTAAAGAAGTAACGGAA+GGG - tig0020281:96260-96279 Msa1379700:intron 35.0%
TATGGTTTAACTGAATCCTC+TGG - tig0020281:96322-96341 Msa1379700:CDS 35.0%
!! TTAACTGAATCCTCTGGAAT+TGG - tig0020281:96328-96347 Msa1379700:CDS 35.0%
AACGAACCTTTCATAATGGT+GGG + tig0020281:96496-96515 None:intergenic 35.0%
AAACGAACCTTTCATAATGG+TGG + tig0020281:96497-96516 None:intergenic 35.0%
GAATTGCTAAACAAGTTTCC+GGG + tig0020281:96693-96712 None:intergenic 35.0%
TTGATGAGAATGAGAACATG+TGG - tig0020281:96742-96761 Msa1379700:intron 35.0%
AATGAGAACATGTGGTCTAT+TGG - tig0020281:96750-96769 Msa1379700:intron 35.0%
AAAGTTTGAGAGGGTAAAGT+TGG + tig0020281:96882-96901 None:intergenic 35.0%
TGTGTGAGAAAAGTTTGAGA+GGG + tig0020281:96891-96910 None:intergenic 35.0%
TTGTGTGAGAAAAGTTTGAG+AGG + tig0020281:96892-96911 None:intergenic 35.0%
TTGGCACACAGATTAAGAAA+TGG + tig0020281:96999-97018 None:intergenic 35.0%
!! TTCAAAATGAGTGTCGCTTT+AGG + tig0020281:97024-97043 None:intergenic 35.0%
!!! GACACTCATTTTGAAACAGA+GGG - tig0020281:97029-97048 Msa1379700:intron 35.0%
ACTCACACAAATGGATATTG+GGG - tig0020281:97140-97159 Msa1379700:intron 35.0%
TAGATGCAAAGGAAAAAGCT+TGG - tig0020281:97356-97375 Msa1379700:intron 35.0%
TAATACCAGCAACTAACCAA+AGG + tig0020281:97386-97405 None:intergenic 35.0%
!! GTTTGCTATATTGATAGCGA+TGG - tig0020281:97547-97566 Msa1379700:CDS 35.0%
GAAAGAGCTCATTAAGTACA+AGG - tig0020281:97594-97613 Msa1379700:CDS 35.0%
CATTAAGTACAAGGGATATC+AGG - tig0020281:97603-97622 Msa1379700:CDS 35.0%
TATCAGGTATCCAAATAAGG+AGG - tig0020281:98024-98043 Msa1379700:CDS 35.0%
CTGCAACAAAATCCATAACT+TGG + tig0020281:98108-98127 None:intergenic 35.0%
TCTATACCCAAAAATCCATC+TGG - tig0020281:98285-98304 Msa1379700:CDS 35.0%
! TTCCGAAGAATTTTACCAGA+TGG + tig0020281:98303-98322 None:intergenic 35.0%
ATCTGGTAAAATTCTTCGGA+AGG - tig0020281:98302-98321 Msa1379700:CDS 35.0%
TTATTCTCTATAAGGGAAGC+AGG + tig0020281:98486-98505 None:intergenic 35.0%
! AGAGTTGTTGGTAGGTGAAT+TGG + tig0020281:95478-95497 None:intergenic 40.0%
AATTCACCTACCAACAACTC+TGG - tig0020281:95477-95496 Msa1379700:three_prime_UTR 40.0%
ACAGGGAAGTAAATGGAGTT+GGG + tig0020281:95578-95597 None:intergenic 40.0%
AACAGGGAAGTAAATGGAGT+TGG + tig0020281:95579-95598 None:intergenic 40.0%
ATTTCGCGAGATGTATTGAG+GGG + tig0020281:95650-95669 None:intergenic 40.0%
GATTTCGCGAGATGTATTGA+GGG + tig0020281:95651-95670 None:intergenic 40.0%
AGTGAAAGCAAGAACAGGTT+TGG + tig0020281:95693-95712 None:intergenic 40.0%
!! TGTTGTTGATGATGAGTTGC+TGG + tig0020281:95783-95802 None:intergenic 40.0%
GAAGATACTACTCCTTTGCT+TGG + tig0020281:95911-95930 None:intergenic 40.0%
CATCGCTATGAGATTCTTGT+GGG + tig0020281:95933-95952 None:intergenic 40.0%
!! AGCGATGGTTCAAGTTGTAA+TGG - tig0020281:95946-95965 Msa1379700:intron 40.0%
TTCAGCATAGCAACCAGTAT+CGG + tig0020281:96169-96188 None:intergenic 40.0%
TTGGAAGAGAGTCGTAAGTA+TGG - tig0020281:96367-96386 Msa1379700:CDS 40.0%
AGAGAGTCGTAAGTATGGTA+CGG - tig0020281:96372-96391 Msa1379700:CDS 40.0%
GGAAAGACAAAACATCCAGA+TGG - tig0020281:96603-96622 Msa1379700:intron 40.0%
TATAAACTCGTGGATCCATC+TGG + tig0020281:96621-96640 None:intergenic 40.0%
!!! GACACTCATTTTGAAACGGA+TGG + tig0020281:96787-96806 None:intergenic 40.0%
!!! CGACACTCATTTTGAAACAG+AGG - tig0020281:97028-97047 Msa1379700:intron 40.0%
TGAAACAGAGGGAGTAATGT+TGG - tig0020281:97040-97059 Msa1379700:intron 40.0%
GTTGGTCATATGCTTGACTT+AGG - tig0020281:97058-97077 Msa1379700:intron 40.0%
! ACTGTTTGAGTTCGAACTTG+TGG - tig0020281:97204-97223 Msa1379700:intron 40.0%
! CTGTTTGAGTTCGAACTTGT+GGG - tig0020281:97205-97224 Msa1379700:intron 40.0%
AGTCATGCACGTCTAATGAA+TGG + tig0020281:97308-97327 None:intergenic 40.0%
! AGGTTATTTCAGTAACGAGG+AGG - tig0020281:97477-97496 Msa1379700:CDS 40.0%
!! CAGAGGGTTGGTTAAAAACA+GGG - tig0020281:97521-97540 Msa1379700:CDS 40.0%
!! AGAGGGTTGGTTAAAAACAG+GGG - tig0020281:97522-97541 Msa1379700:CDS 40.0%
! CGATGGATTCTTATTTGTCG+TGG - tig0020281:97564-97583 Msa1379700:CDS 40.0%
TAGTTGAAGTGATCGAAACG+TGG + tig0020281:97671-97690 None:intergenic 40.0%
AGCGGTCTTGATTGATAAGA+AGG + tig0020281:97983-98002 None:intergenic 40.0%
TTCCAATGGCATATGTGGTA+AGG - tig0020281:98056-98075 Msa1379700:CDS 40.0%
TTCCTTACCACATATGCCAT+TGG + tig0020281:98061-98080 None:intergenic 40.0%
AATGGCATATGTGGTAAGGA+AGG - tig0020281:98060-98079 Msa1379700:CDS 40.0%
! TATGGATTTTGTTGCAGAGC+AGG - tig0020281:98111-98130 Msa1379700:CDS 40.0%
AGAGTGATCGATCCAAGAAG+TGG - tig0020281:95311-95330 Msa1379700:three_prime_UTR 45.0%
GAGTGATCGATCCAAGAAGT+GGG - tig0020281:95312-95331 Msa1379700:three_prime_UTR 45.0%
!! AAAGAGTGGTTTGGTGGAAG+TGG + tig0020281:95383-95402 None:intergenic 45.0%
! GCATCTAAAGAGTGGTTTGG+TGG + tig0020281:95389-95408 None:intergenic 45.0%
GTGGCATCTAAAGAGTGGTT+TGG + tig0020281:95392-95411 None:intergenic 45.0%
AAGTGGTGGCATCTAAAGAG+TGG + tig0020281:95397-95416 None:intergenic 45.0%
! GTTGTTGGTAGGTGAATTGG+TGG + tig0020281:95475-95494 None:intergenic 45.0%
ACTTCCTCACTCTCCAACAT+GGG - tig0020281:95518-95537 Msa1379700:three_prime_UTR 45.0%
AGTAAATGGAGTTGGGAGAG+AGG + tig0020281:95571-95590 None:intergenic 45.0%
GCAGACAACAGGGAAGTAAA+TGG + tig0020281:95585-95604 None:intergenic 45.0%
AGATGTATTGAGGGGATTGG+TGG + tig0020281:95642-95661 None:intergenic 45.0%
CGATTTCGCGAGATGTATTG+AGG + tig0020281:95652-95671 None:intergenic 45.0%
GGAGTAGTGAAAGCAAGAAC+AGG + tig0020281:95698-95717 None:intergenic 45.0%
TGGAAGTGAAGGTGATGCTT+GGG + tig0020281:95741-95760 None:intergenic 45.0%
TTGGAAGTGAAGGTGATGCT+TGG + tig0020281:95742-95761 None:intergenic 45.0%
GTGGTGTCCATGAGAATGAT+TGG + tig0020281:95761-95780 None:intergenic 45.0%
!! TGTTGATGATGAGTTGCTGG+TGG + tig0020281:95780-95799 None:intergenic 45.0%
TCAGTCACTCTACTCAACTC+AGG + tig0020281:95833-95852 None:intergenic 45.0%
TCCTGATGAGTAGAGTAGTG+TGG + tig0020281:95879-95898 None:intergenic 45.0%
CTCTACTCATCAGGAACAAC+TGG - tig0020281:95884-95903 Msa1379700:intron 45.0%
CCATCGCTATGAGATTCTTG+TGG + tig0020281:95934-95953 None:intergenic 45.0%
CCACAAGAATCTCATAGCGA+TGG - tig0020281:95931-95950 Msa1379700:intron 45.0%
!! GGTCTTGCAGTGTTTGCTAT+GGG - tig0020281:96037-96056 Msa1379700:CDS 45.0%
!! GTCTTGCAGTGTTTGCTATG+GGG - tig0020281:96038-96057 Msa1379700:CDS 45.0%
! TTTGAGTTCGCTTCATACGG+TGG - tig0020281:96219-96238 Msa1379700:intron 45.0%
AGTAGAAGCTCCAATTCCAG+AGG + tig0020281:96341-96360 None:intergenic 45.0%
TGGAGCTTCTACTGAGTCTT+TGG - tig0020281:96348-96367 Msa1379700:CDS 45.0%
CTCAGCCGTTACCAGTTAAT+CGG - tig0020281:96446-96465 Msa1379700:intron 45.0%
CCAGTCCGATTAACTGGTAA+CGG + tig0020281:96454-96473 None:intergenic 45.0%
!! TTAATCGGACTGGTGAGCTT+TGG - tig0020281:96461-96480 Msa1379700:intron 45.0%
TATTGAACACGAGGTAGACC+CGG - tig0020281:96672-96691 Msa1379700:intron 45.0%
!! ATGAGTGTCGCTTTAGGCTT+TGG + tig0020281:97018-97037 None:intergenic 45.0%
! GAAAAAGCTTGGTGAGCCTT+TGG - tig0020281:97367-97386 Msa1379700:intron 45.0%
!! ATGCAAGACATGGTTGGTGA+TGG - tig0020281:97453-97472 Msa1379700:CDS 45.0%
! GGCAGGTTATTTCAGTAACG+AGG - tig0020281:97474-97493 Msa1379700:CDS 45.0%
ACATCGACCATTACTCCAGA+GGG - tig0020281:97505-97524 Msa1379700:CDS 45.0%
!! TAACCAACCCTCTGGAGTAA+TGG + tig0020281:97515-97534 None:intergenic 45.0%
!!! CCTGTTTTTAACCAACCCTC+TGG + tig0020281:97523-97542 None:intergenic 45.0%
!! CCAGAGGGTTGGTTAAAAAC+AGG - tig0020281:97520-97539 Msa1379700:CDS 45.0%
!! AACAAAGCCTCTAGTTCTGC+TGG + tig0020281:97817-97836 None:intergenic 45.0%
AGGTATCCAAATAAGGAGGC+TGG - tig0020281:98028-98047 Msa1379700:CDS 45.0%
GGTATCCAAATAAGGAGGCT+GGG - tig0020281:98029-98048 Msa1379700:CDS 45.0%
CTTGTCAGGAAGCCAAGTTA+TGG - tig0020281:98093-98112 Msa1379700:CDS 45.0%
! GCAGAGCAGGTTTGTTTGTT+TGG - tig0020281:98124-98143 Msa1379700:CDS 45.0%
!! TATAATTAATTTATATTCTT+TGG + tig0020281:97888-97907 None:intergenic 5.0%
!!! ATTTATTTTTTATAATATGA+TGG - tig0020281:97940-97959 Msa1379700:CDS 5.0%
!!! ATTAATTTTTTTCAATATAT+AGG - tig0020281:98221-98240 Msa1379700:CDS 5.0%
CTGCTGCAAAACCCACTTCT+TGG + tig0020281:95326-95345 None:intergenic 50.0%
!! TGGTTTGGTGGAAGTGGAAG+GGG + tig0020281:95377-95396 None:intergenic 50.0%
!! GTGGTTTGGTGGAAGTGGAA+GGG + tig0020281:95378-95397 None:intergenic 50.0%
!! AGTGGTTTGGTGGAAGTGGA+AGG + tig0020281:95379-95398 None:intergenic 50.0%
AGGCGTCAATGAAGGCGATA+TGG + tig0020281:95445-95464 None:intergenic 50.0%
! TTGGTAGGTGAATTGGTGGC+CGG + tig0020281:95471-95490 None:intergenic 50.0%
GTTGGAGAGTGAGGAAGTGA+CGG + tig0020281:95516-95535 None:intergenic 50.0%
CACTTCCTCACTCTCCAACA+TGG - tig0020281:95517-95536 Msa1379700:three_prime_UTR 50.0%
CTTCCTCACTCTCCAACATG+GGG - tig0020281:95519-95538 Msa1379700:three_prime_UTR 50.0%
TCCAACATGGGGATCCGTAA+AGG - tig0020281:95530-95549 Msa1379700:three_prime_UTR 50.0%
ACCTTTACGGATCCCCATGT+TGG + tig0020281:95534-95553 None:intergenic 50.0%
GGAGGATGACGTCACCTTTA+CGG + tig0020281:95547-95566 None:intergenic 50.0%
AAATGGAGTTGGGAGAGAGG+AGG + tig0020281:95568-95587 None:intergenic 50.0%
! TGTATTGAGGGGATTGGTGG+TGG + tig0020281:95639-95658 None:intergenic 50.0%
GCGAGATGTATTGAGGGGAT+TGG + tig0020281:95645-95664 None:intergenic 50.0%
GTAGTGTGGCTGTATCATCC+TGG + tig0020281:95865-95884 None:intergenic 50.0%
GCCACACTACTCTACTCATC+AGG - tig0020281:95875-95894 Msa1379700:intron 50.0%
GGAACAACTGGACCAAGCAA+AGG - tig0020281:95896-95915 Msa1379700:intron 50.0%
! CGGTCTTGCAGTGTTTGCTA+TGG - tig0020281:96036-96055 Msa1379700:intron 50.0%
TGCTATGGGGCTTCTTGCTA+TGG - tig0020281:96051-96070 Msa1379700:CDS 50.0%
! GCTATGGGGCTTCTTGCTAT+GGG - tig0020281:96052-96071 Msa1379700:CDS 50.0%
! AGCATAGCAACCAGTATCGG+CGG + tig0020281:96166-96185 None:intergenic 50.0%
TCGCTTCATACGGTGGTTTC+TGG - tig0020281:96226-96245 Msa1379700:intron 50.0%
! CTTCATACGGTGGTTTCTGG+TGG - tig0020281:96229-96248 Msa1379700:intron 50.0%
GAGTCGTAAGTATGGTACGG+CGG - tig0020281:96375-96394 Msa1379700:CDS 50.0%
AGTCGTAAGTATGGTACGGC+GGG - tig0020281:96376-96395 Msa1379700:CDS 50.0%
GTCAACAATCATAGCCTCCG+TGG + tig0020281:96416-96435 None:intergenic 50.0%
CCGTTACCAGTTAATCGGAC+TGG - tig0020281:96451-96470 Msa1379700:intron 50.0%
AGCTCACCAGTCCGATTAAC+TGG + tig0020281:96460-96479 None:intergenic 50.0%
CGTGGTCCCACCATTATGAA+AGG - tig0020281:96487-96506 Msa1379700:intron 50.0%
GGATATTGGGGATCGAATCC+TGG - tig0020281:97152-97171 Msa1379700:intron 50.0%
ATTGTTAGGCAGAGCACACC+AGG + tig0020281:97173-97192 None:intergenic 50.0%
TAGACGTGCATGACTCTCCA+AGG - tig0020281:97313-97332 Msa1379700:intron 50.0%
! GTGAGCCTTTGGTTAGTTGC+TGG - tig0020281:97378-97397 Msa1379700:intron 50.0%
!! AAGACATGGTTGGTGATGGC+AGG - tig0020281:97457-97476 Msa1379700:CDS 50.0%
!! CTCTGGAGTAATGGTCGATG+TGG + tig0020281:97506-97525 None:intergenic 50.0%
CACATCGACCATTACTCCAG+AGG - tig0020281:97504-97523 Msa1379700:CDS 50.0%
!! AAGCCTCTAGTTCTGCTGGA+GGG + tig0020281:97813-97832 None:intergenic 50.0%
!! AAAGCCTCTAGTTCTGCTGG+AGG + tig0020281:97814-97833 None:intergenic 50.0%
ACAGCAGCGTCTACAATAGC+AGG + tig0020281:97850-97869 None:intergenic 50.0%
GCAGGTTCCAATGGCATATG+TGG - tig0020281:98051-98070 Msa1379700:CDS 50.0%
GCATATGTGGTAAGGAAGGC+TGG - tig0020281:98064-98083 Msa1379700:CDS 50.0%
! AAGGCTGGAAGTAGCTTGTC+AGG - tig0020281:98079-98098 Msa1379700:CDS 50.0%
! GAAGCATTCAGGAATGCAGC+AGG + tig0020281:98464-98483 None:intergenic 50.0%
!! AAGGGAAGCAGGAAGCATTC+AGG + tig0020281:98475-98494 None:intergenic 50.0%
AGCACGCCAGAGTTGTTGGT+AGG + tig0020281:95486-95505 None:intergenic 55.0%
CTACAGCACGCCAGAGTTGT+TGG + tig0020281:95490-95509 None:intergenic 55.0%
GATCCCCATGTTGGAGAGTG+AGG + tig0020281:95525-95544 None:intergenic 55.0%
TGACGGAGAGGCAGACAACA+GGG + tig0020281:95595-95614 None:intergenic 55.0%
! ATGACGGAGAGGCAGACAAC+AGG + tig0020281:95596-95615 None:intergenic 55.0%
AGTGACTGAGCGCATCAACC+AGG - tig0020281:95844-95863 Msa1379700:CDS 55.0%
GTCGTAAGTATGGTACGGCG+GGG - tig0020281:96377-96396 Msa1379700:CDS 55.0%
! ACTGGTGAGCTTTGGCTTCG+TGG - tig0020281:96469-96488 Msa1379700:intron 55.0%
CGACCATTACTCCAGAGGGT+TGG - tig0020281:97509-97528 Msa1379700:CDS 55.0%
TCCAAATAAGGAGGCTGGGC+AGG - tig0020281:98033-98052 Msa1379700:CDS 55.0%
ACCTGCCCAGCCTCCTTATT+TGG + tig0020281:98037-98056 None:intergenic 55.0%
GCGGGAGGAGATGAAAGTGG+TGG + tig0020281:95411-95430 None:intergenic 60.0%
AGCGCGGGAGGAGATGAAAG+TGG + tig0020281:95414-95433 None:intergenic 60.0%
ATCTCCTCCCGCGCTCATCA+CGG - tig0020281:95419-95438 Msa1379700:three_prime_UTR 60.0%
GATATGGCCGTGATGAGCGC+GGG + tig0020281:95429-95448 None:intergenic 60.0%
CGATATGGCCGTGATGAGCG+CGG + tig0020281:95430-95449 None:intergenic 60.0%
GCCTTCATTGACGCCTCCAC+CGG - tig0020281:95449-95468 Msa1379700:three_prime_UTR 60.0%
! GTAGGTGAATTGGTGGCCGG+TGG + tig0020281:95468-95487 None:intergenic 60.0%
TGGAGTTGGGAGAGAGGAGG+AGG + tig0020281:95565-95584 None:intergenic 60.0%
TGCCTCTCCGTCATGTCCCT+CGG - tig0020281:95602-95621 Msa1379700:CDS 60.0%
! GATAGCGCCGAGGGACATGA+CGG + tig0020281:95612-95631 None:intergenic 60.0%
! GGTGGTGATGATAGCGCCGA+GGG + tig0020281:95621-95640 None:intergenic 60.0%
! TGGTGGTGATGATAGCGCCG+AGG + tig0020281:95622-95641 None:intergenic 60.0%
CGGCGGCACAAGTGGTAAGA+AGG + tig0020281:96149-96168 None:intergenic 60.0%
ACCACTTGTGCCGCCGATAC+TGG - tig0020281:96153-96172 Msa1379700:intron 60.0%
!! ACCAGTATCGGCGGCACAAG+TGG + tig0020281:96157-96176 None:intergenic 60.0%
GGGGCTTGTGTCTGCTTCCA+CGG - tig0020281:96396-96415 Msa1379700:CDS 60.0%
GCTTGTGTCTGCTTCCACGG+AGG - tig0020281:96399-96418 Msa1379700:CDS 60.0%
GGTCCCTCCAGCAGAACTAG+AGG - tig0020281:97807-97826 Msa1379700:CDS 60.0%
!! GCCGGTGGAGGCGTCAATGA+AGG + tig0020281:95453-95472 None:intergenic 65.0%
! GGTGAATTGGTGGCCGGTGG+AGG + tig0020281:95465-95484 None:intergenic 65.0%
GGAGGCTGGGCAGGTTCCAA+TGG - tig0020281:98042-98061 Msa1379700:CDS 65.0%
ATGGCCGTGATGAGCGCGGG+AGG + tig0020281:95426-95445 None:intergenic 70.0%
! CGCCGAGGGACATGACGGAG+AGG + tig0020281:95607-95626 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0020281 gene 95115 98797 95115 ID=Msa1379700;Name=Msa1379700
tig0020281 mRNA 95115 98797 95115 ID=Msa1379700-mRNA-1;Parent=Msa1379700;Name=Msa1379700-mRNA-1;_AED=0.05;_eAED=0.05;_QI=163|1|1|1|1|1|5|441|553
tig0020281 exon 95115 95669 95115 ID=Msa1379700-mRNA-1:exon:6722;Parent=Msa1379700-mRNA-1
tig0020281 exon 95780 95882 95780 ID=Msa1379700-mRNA-1:exon:6721;Parent=Msa1379700-mRNA-1
tig0020281 exon 96037 96104 96037 ID=Msa1379700-mRNA-1:exon:6720;Parent=Msa1379700-mRNA-1
tig0020281 exon 96288 96433 96288 ID=Msa1379700-mRNA-1:exon:6719;Parent=Msa1379700-mRNA-1
tig0020281 exon 97404 98797 97404 ID=Msa1379700-mRNA-1:exon:6718;Parent=Msa1379700-mRNA-1
tig0020281 five_prime_UTR 98635 98797 98635 ID=Msa1379700-mRNA-1:five_prime_utr;Parent=Msa1379700-mRNA-1
tig0020281 CDS 97404 98634 97404 ID=Msa1379700-mRNA-1:cds;Parent=Msa1379700-mRNA-1
tig0020281 CDS 96288 96433 96288 ID=Msa1379700-mRNA-1:cds;Parent=Msa1379700-mRNA-1
tig0020281 CDS 96037 96104 96037 ID=Msa1379700-mRNA-1:cds;Parent=Msa1379700-mRNA-1
tig0020281 CDS 95780 95882 95780 ID=Msa1379700-mRNA-1:cds;Parent=Msa1379700-mRNA-1
tig0020281 CDS 95556 95669 95556 ID=Msa1379700-mRNA-1:cds;Parent=Msa1379700-mRNA-1
tig0020281 three_prime_UTR 95115 95555 95115 ID=Msa1379700-mRNA-1:three_prime_utr;Parent=Msa1379700-mRNA-1
Gene Sequence

>Msa1379700

ATGGAGGTAAACGGTGAGAATACGAATAGCAGAAGAGTGATCGATCCAAGAAGTGGGTTTTGCAGCAGCAATTCAATCTTCTACAGCAAGAGAAAACCCCTTCCACTTCCACCAAACCACTCTTTAGATGCCACCACTTTCATCTCCTCCCGCGCTCATCACGGCCATATCGCCTTCATTGACGCCTCCACCGGCCACCAATTCACCTACCAACAACTCTGGCGTGCTGTAGACTCCGTCACTTCCTCACTCTCCAACATGGGGATCCGTAAAGGTGACGTCATCCTCCTCCTCTCTCCCAACTCCATTTACTTCCCTGTTGTCTGCCTCTCCGTCATGTCCCTCGGCGCTATCATCACCACCACCAATCCCCTCAATACATCTCGCGAAATCGCTAAACAGATCGCTGATTCCAAACCTGTTCTTGCTTTCACTACTCCACAACTCCTTTCTAAAATCACCCAAGCATCACCTTCACTTCCAATCATTCTCATGGACACCACCAGCAACTCATCATCAACAACAACACTTGATGAAATGATGAAGAAAGAACCTGAGTTGAGTAGAGTGACTGAGCGCATCAACCAGGATGATACAGCCACACTACTCTACTCATCAGGAACAACTGGACCAAGCAAAGGAGTAGTATCTTCCCACAAGAATCTCATAGCGATGGTTCAAGTTGTAATGGCTAGATTTGGTAAAGAACAAGAGACGAGAGAAACTTTCATATGCACTGTTCCGATGTTTCATATATACGGTCTTGCAGTGTTTGCTATGGGGCTTCTTGCTATGGGTTCAACCATAGTTGTTCTCTCTAAATTCGAGATGCATGACATGCTTTCATCTATTGAGAAATTCAGAGCAACCTTCTTACCACTTGTGCCGCCGATACTGGTTGCTATGCTGAACAACGCAGATGCGATTAAGAGAAAGTATGATTTGAGTTCGCTTCATACGGTGGTTTCTGGTGGTGCTCCTTTGAGTAAAGAAGTAACGGAAGGGTTTATAGAAAAGTATCCTAATGTAAGTATCTTTCAAGGTTATGGTTTAACTGAATCCTCTGGAATTGGAGCTTCTACTGAGTCTTTGGAAGAGAGTCGTAAGTATGGTACGGCGGGGCTTGTGTCTGCTTCCACGGAGGCTATGATTGTTGACACTGAGACTGCTCAGCCGTTACCAGTTAATCGGACTGGTGAGCTTTGGCTTCGTGGTCCCACCATTATGAAAGGTTATTTCAGTAACGAGGAGGCAACCACATCGACCATTACTCCAGAGGGTTGGTTAAAAACAGGGGATGTTTGCTATATTGATAGCGATGGATTCTTATTTGTCGTGGATCGCTTGAAAGAGCTCATTAAGTACAAGGGATATCAGGTCCCTCCAGCAGAACTAGAGGCTTTGTTGCTGACTCATCCTGCTATTGTAGACGCTGCTGTCATACCGTATCCAAATAAGGAGGCTGGGCAGGTTCCAATGGCATATGTGGTAAGGAAGGCTGGAAGTAGCTTGTCAGGAAGCCAAGTTATGGATTTTGTTGCAGAGCAGGTGGCTCCATACAAAAGAATTCGAAAAGTGGCATTTATATCTTCTATACCCAAAAATCCATCTGGTAAAATTCTTCGGAAGGATCTTATCAAGCTAGCTACGTCTAAACTTTGA
Protein sequence

>Msa1379700

MEVNGENTNSRRVIDPRSGFCSSNSIFYSKRKPLPLPPNHSLDATTFISSRAHHGHIAFIDASTGHQFTYQQLWRAVDSVTSSLSNMGIRKGDVILLLSPNSIYFPVVCLSVMSLGAIITTTNPLNTSREIAKQIADSKPVLAFTTPQLLSKITQASPSLPIILMDTTSNSSSTTTLDEMMKKEPELSRVTERINQDDTATLLYSSGTTGPSKGVVSSHKNLIAMVQVVMARFGKEQETRETFICTVPMFHIYGLAVFAMGLLAMGSTIVVLSKFEMHDMLSSIEKFRATFLPLVPPILVAMLNNADAIKRKYDLSSLHTVVSGGAPLSKEVTEGFIEKYPNVSIFQGYGLTESSGIGASTESLEESRKYGTAGLVSASTEAMIVDTETAQPLPVNRTGELWLRGPTIMKGYFSNEEATTSTITPEGWLKTGDVCYIDSDGFLFVVDRLKELIKYKGYQVPPAELEALLLTHPAIVDAAVIPYPNKEAGQVPMAYVVRKAGSSLSGSQVMDFVAEQVAPYKRIRKVAFISSIPKNPSGKILRKDLIKLATSKL*