Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1372700 | XP_013453298.1 | 98.765 | 81 | 1 | 0 | 1 | 81 | 1 | 81 | 1.37e-51 | 166 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1372700 | sp|Q1G3M2|LYRM9_ARATH | 72.500 | 80 | 21 | 1 | 1 | 80 | 1 | 79 | 4.78e-35 | 117 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1372700 | A0A072UCK1 | 98.765 | 81 | 1 | 0 | 1 | 81 | 1 | 81 | 6.54e-52 | 166 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0610390 | Msa1372700 | 0.807112 | 5.926808e-50 | -8.615850e-47 |
| Msa0650920 | Msa1372700 | 0.820004 | 8.621843e-53 | -8.615850e-47 |
| Msa0661300 | Msa1372700 | 0.807712 | 4.420452e-50 | -8.615850e-47 |
| Msa0718740 | Msa1372700 | 0.801447 | 8.965225e-49 | -8.615850e-47 |
| Msa0763020 | Msa1372700 | 0.811335 | 7.372076e-51 | -8.615850e-47 |
| Msa0763070 | Msa1372700 | 0.819057 | 1.418576e-52 | -8.615850e-47 |
| Msa0832240 | Msa1372700 | 0.970804 | 3.544278e-132 | -8.615850e-47 |
| Msa0194480 | Msa1372700 | 0.804392 | 2.208113e-49 | -8.615850e-47 |
| Msa1138870 | Msa1372700 | 0.801664 | 8.093297e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1372700 | MtrunA17_Chr5g0393631 | 98.765 | 81 | 1 | 0 | 1 | 81 | 1 | 81 | 1.26e-55 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1372700 | AT3G19508.1 | 72.500 | 80 | 21 | 1 | 1 | 80 | 1 | 79 | 4.85e-36 | 117 |
Find 33 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGAGAAGAGTTAGATTAGA+TGG | 0.284074 | tig0019314:+33614 | Msa1372700:exon |
| GATATGGAAACTCATTCTTT+AGG | 0.300017 | tig0019314:-34080 | None:intergenic |
| TTCTCTATGGGTTCTTCATA+AGG | 0.314427 | tig0019314:+33806 | Msa1372700:CDS |
| GTACCTACACTCATTCTCTA+TGG | 0.327232 | tig0019314:+33793 | Msa1372700:CDS |
| CTCTTATTGAATCAAGGATA+TGG | 0.337434 | tig0019314:-34096 | None:intergenic |
| TAGTTGGAAGGAAGGAAGTT+TGG | 0.347652 | tig0019314:+33584 | None:intergenic |
| TAATAACCTCTTGAATCCTT+TGG | 0.385434 | tig0019314:-33687 | None:intergenic |
| ACTCATTCTTTAGGAAATGT+TGG | 0.406919 | tig0019314:-34071 | None:intergenic |
| AGCTCAAGGTGATATGTTGC+GGG | 0.429923 | tig0019314:+33940 | Msa1372700:CDS |
| AGTTAAGTTAGTTGGAAGGA+AGG | 0.438224 | tig0019314:+33576 | None:intergenic |
| CGGTAACTCTTATTGAATCA+AGG | 0.492106 | tig0019314:-34102 | None:intergenic |
| TGGAAGGAAGGAAGTTTGGA+AGG | 0.495000 | tig0019314:+33588 | None:intergenic |
| AGGTACGTTGAAAGAGATCA+TGG | 0.523995 | tig0019314:-33776 | None:intergenic |
| CAGTTGAGGTACACAGATGT+TGG | 0.528997 | tig0019314:-33968 | None:intergenic |
| AGTTGAGGTACACAGATGTT+GGG | 0.540166 | tig0019314:-33967 | None:intergenic |
| GAAGGAAGTTTGGAAGGGAA+AGG | 0.544418 | tig0019314:+33594 | Msa1372700:five_prime_UTR |
| AAAGAGATCATGGAGAGTAG+TGG | 0.552251 | tig0019314:-33766 | None:intergenic |
| TACCTACACTCATTCTCTAT+GGG | 0.556578 | tig0019314:+33794 | Msa1372700:CDS |
| GTTAGATTAGATGGAGAAAG+CGG | 0.560882 | tig0019314:+33623 | Msa1372700:exon |
| GGAAGGAAGGAAGTTTGGAA+GGG | 0.562383 | tig0019314:+33589 | Msa1372700:five_prime_UTR |
| AAGCTCAAGGTGATATGTTG+CGG | 0.562731 | tig0019314:+33939 | Msa1372700:CDS |
| ACTTGTGCGGCGTTTACCAA+AGG | 0.567162 | tig0019314:+33671 | Msa1372700:CDS |
| CGTTTACCAAAGGATTCAAG+AGG | 0.591068 | tig0019314:+33681 | Msa1372700:CDS |
| ATCTGCCGCTGATAAGCTCA+AGG | 0.615005 | tig0019314:+33926 | Msa1372700:CDS |
| TATCTGTATATATATACTCA+CGG | 0.619326 | tig0019314:-34125 | None:intergenic |
| ACAAGCAGTATTGTTATGTG+AGG | 0.635803 | tig0019314:-34003 | None:intergenic |
| TGGAGAGTAGTGGAATCAGA+AGG | 0.642946 | tig0019314:-33756 | None:intergenic |
| AATCAGAAGGATCAACCTCA+CGG | 0.688438 | tig0019314:-33743 | None:intergenic |
| CAGAAGTTCTGAGACTTGTG+CGG | 0.691962 | tig0019314:+33658 | Msa1372700:CDS |
| GAACTTCGTTAATTACCGTG+AGG | 0.698092 | tig0019314:+33728 | Msa1372700:CDS |
| TATCACCTTGAGCTTATCAG+CGG | 0.704627 | tig0019314:-33931 | None:intergenic |
| AACCCATAGAGAATGAGTGT+AGG | 0.739379 | tig0019314:-33796 | None:intergenic |
| CTGTATATATATACTCACGG+CGG | 0.821168 | tig0019314:-34122 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATCTGTATATATATACTCA+CGG | - | tig0019314:34128-34147 | None:intergenic | 20.0% |
| TAATAACCTCTTGAATCCTT+TGG | - | tig0019314:33690-33709 | None:intergenic | 30.0% | |
| CACTCATTCATTTCTGTTTA+TGG | + | tig0019314:33836-33855 | Msa1372700:intron | 30.0% | |
| ! | ACTCATTCTTTAGGAAATGT+TGG | - | tig0019314:34074-34093 | None:intergenic | 30.0% |
| GATATGGAAACTCATTCTTT+AGG | - | tig0019314:34083-34102 | None:intergenic | 30.0% | |
| CTCTTATTGAATCAAGGATA+TGG | - | tig0019314:34099-34118 | None:intergenic | 30.0% | |
| AGGAGAAGAGTTAGATTAGA+TGG | + | tig0019314:33614-33633 | Msa1372700:exon | 35.0% | |
| GTTAGATTAGATGGAGAAAG+CGG | + | tig0019314:33623-33642 | Msa1372700:exon | 35.0% | |
| TACCTACACTCATTCTCTAT+GGG | + | tig0019314:33794-33813 | Msa1372700:CDS | 35.0% | |
| TTCTCTATGGGTTCTTCATA+AGG | + | tig0019314:33806-33825 | Msa1372700:CDS | 35.0% | |
| GGAAAAACAACAAACAGTTG+AGG | - | tig0019314:33985-34004 | None:intergenic | 35.0% | |
| ACAAGCAGTATTGTTATGTG+AGG | - | tig0019314:34006-34025 | None:intergenic | 35.0% | |
| CGGTAACTCTTATTGAATCA+AGG | - | tig0019314:34105-34124 | None:intergenic | 35.0% | |
| CTGTATATATATACTCACGG+CGG | - | tig0019314:34125-34144 | None:intergenic | 35.0% | |
| CGTTTACCAAAGGATTCAAG+AGG | + | tig0019314:33681-33700 | Msa1372700:CDS | 40.0% | |
| GAACTTCGTTAATTACCGTG+AGG | + | tig0019314:33728-33747 | Msa1372700:CDS | 40.0% | |
| AATCAGAAGGATCAACCTCA+CGG | - | tig0019314:33746-33765 | None:intergenic | 40.0% | |
| AAAGAGATCATGGAGAGTAG+TGG | - | tig0019314:33769-33788 | None:intergenic | 40.0% | |
| AGGTACGTTGAAAGAGATCA+TGG | - | tig0019314:33779-33798 | None:intergenic | 40.0% | |
| GTACCTACACTCATTCTCTA+TGG | + | tig0019314:33793-33812 | Msa1372700:CDS | 40.0% | |
| AACCCATAGAGAATGAGTGT+AGG | - | tig0019314:33799-33818 | None:intergenic | 40.0% | |
| TGCAATGCTACTGCAGTATT+CGG | + | tig0019314:33896-33915 | Msa1372700:intron | 40.0% | |
| TATCACCTTGAGCTTATCAG+CGG | - | tig0019314:33934-33953 | None:intergenic | 40.0% | |
| AAGCTCAAGGTGATATGTTG+CGG | + | tig0019314:33939-33958 | Msa1372700:CDS | 40.0% | |
| AGTTGAGGTACACAGATGTT+GGG | - | tig0019314:33970-33989 | None:intergenic | 40.0% | |
| GGAAGGAAGGAAGTTTGGAA+GGG | + | tig0019314:33589-33608 | Msa1372700:five_prime_UTR | 45.0% | |
| GAAGGAAGTTTGGAAGGGAA+AGG | + | tig0019314:33594-33613 | Msa1372700:five_prime_UTR | 45.0% | |
| !! | CAGAAGTTCTGAGACTTGTG+CGG | + | tig0019314:33658-33677 | Msa1372700:CDS | 45.0% |
| ! | TGGAGAGTAGTGGAATCAGA+AGG | - | tig0019314:33759-33778 | None:intergenic | 45.0% |
| AGCTCAAGGTGATATGTTGC+GGG | + | tig0019314:33940-33959 | Msa1372700:CDS | 45.0% | |
| CAGTTGAGGTACACAGATGT+TGG | - | tig0019314:33971-33990 | None:intergenic | 45.0% | |
| ACTTGTGCGGCGTTTACCAA+AGG | + | tig0019314:33671-33690 | Msa1372700:CDS | 50.0% | |
| ATCTGCCGCTGATAAGCTCA+AGG | + | tig0019314:33926-33945 | Msa1372700:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0019314 | gene | 33589 | 34176 | 33589 | ID=Msa1372700;Name=Msa1372700 |
| tig0019314 | mRNA | 33589 | 34176 | 33589 | ID=Msa1372700-mRNA-1;Parent=Msa1372700;Name=Msa1372700-mRNA-1;_AED=0.07;_eAED=0.07;_QI=44|1|1|1|1|1|2|211|82 |
| tig0019314 | exon | 33589 | 33827 | 33589 | ID=Msa1372700-mRNA-1:exon:2869;Parent=Msa1372700-mRNA-1 |
| tig0019314 | exon | 33912 | 34176 | 33912 | ID=Msa1372700-mRNA-1:exon:2870;Parent=Msa1372700-mRNA-1 |
| tig0019314 | five_prime_UTR | 33589 | 33632 | 33589 | ID=Msa1372700-mRNA-1:five_prime_utr;Parent=Msa1372700-mRNA-1 |
| tig0019314 | CDS | 33633 | 33827 | 33633 | ID=Msa1372700-mRNA-1:cds;Parent=Msa1372700-mRNA-1 |
| tig0019314 | CDS | 33912 | 33965 | 33912 | ID=Msa1372700-mRNA-1:cds;Parent=Msa1372700-mRNA-1 |
| tig0019314 | three_prime_UTR | 33966 | 34176 | 33966 | ID=Msa1372700-mRNA-1:three_prime_utr;Parent=Msa1372700-mRNA-1 |
| Gene Sequence |
| Protein sequence |
