AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0160620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 96 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGCAGGGTTGACGATTC+AGG 0.139753 1_4:+33688034 Msa0160620:five_prime_UTR
CGCCACTTCAATGCATGAAT+TGG 0.208421 1_4:-33686629 None:intergenic
TAAGATTACATGTATCATTT+TGG 0.226865 1_4:-33686488 None:intergenic
TCATATCATATAAAGAAAAT+AGG 0.261721 1_4:-33688995 None:intergenic
TGTTTCAATTTCTCTACTTT+GGG 0.267804 1_4:+33686666 Msa0160620:five_prime_UTR
GGTGCTTAAAATTTATTATC+TGG 0.297194 1_4:+33688709 Msa0160620:three_prime_UTR
ATTGGGATAAGAAAGAAAAT+TGG 0.309564 1_4:-33686611 None:intergenic
TTGTTTCAATTTCTCTACTT+TGG 0.314070 1_4:+33686665 Msa0160620:five_prime_UTR
TTGGGATAAGAAAGAAAATT+GGG 0.319969 1_4:-33686610 None:intergenic
ATTAATGGTGCTGCAAAGTT+TGG 0.320369 1_4:+33688125 Msa0160620:five_prime_UTR
CTACCTTATATGGCAAAATA+TGG 0.320601 1_4:+33688925 Msa0160620:three_prime_UTR
GGAAGCATACGGTCCTTTCT+TGG 0.323111 1_4:+33688372 Msa0160620:CDS
AGGGAGTATGTTCTGATCTT+AGG 0.324742 1_4:-33686395 None:intergenic
GGAAGTCTTGTTGATTTAGT+TGG 0.335009 1_4:+33688340 Msa0160620:CDS
GGTTATTTACATGTAAAATA+TGG 0.347890 1_4:+33688946 Msa0160620:three_prime_UTR
GGTTTCGTGCGGTTGTTTAG+AGG 0.356770 1_4:+33688739 Msa0160620:three_prime_UTR
CAGTTAGGACCGAGGCTTCT+TGG 0.357951 1_4:+33688016 Msa0160620:five_prime_UTR
AATTTCTGCGTAGGAATATT+CGG 0.369204 1_4:+33687770 Msa0160620:intron
TCAAAGGGCTATGGTGGCTT+TGG 0.369380 1_4:-33686449 None:intergenic
AAAATACCTTGAAATCCTTT+GGG 0.373281 1_4:-33688676 None:intergenic
GCAAGAACAAGAAGAATAGA+AGG 0.386139 1_4:+33686862 Msa0160620:five_prime_UTR
ATAAATTACCTAGTCAGAAT+AGG 0.388571 1_4:-33688482 None:intergenic
TTGAATTGTGCTTCTCGCCT+TGG 0.392592 1_4:-33688232 None:intergenic
ATTTGTGTTCCAGATTGTTT+AGG 0.397267 1_4:+33687989 Msa0160620:intron
TGGATGAGGTTACCTTTGAA+TGG 0.410546 1_4:+33688392 Msa0160620:CDS
TAGGACCGAGGCTTCTTGGC+AGG 0.415660 1_4:+33688020 Msa0160620:five_prime_UTR
AATTGCTGCGTAGGAATATT+CGG 0.425916 1_4:+33687892 Msa0160620:five_prime_UTR
GATTGTTTAGGCAGGCAGTT+AGG 0.435522 1_4:+33688001 Msa0160620:intron
TTGGCAGGGTTGACGATTCA+GGG 0.436084 1_4:+33688035 Msa0160620:five_prime_UTR
CTGTAGGTTCGGTTGGGACT+AGG 0.439250 1_4:-33686571 None:intergenic
CGTGTTCGTGCATTTGAAAC+GGG 0.440856 1_4:+33687848 Msa0160620:five_prime_UTR
GAGAGATAGAAAGGAGTAAG+AGG 0.441164 1_4:-33686415 None:intergenic
TGGTTCATCCCCACTTGATA+TGG 0.445421 1_4:+33686922 Msa0160620:five_prime_UTR
TGGTATGGAACTTCTTTCTC+CGG 0.445848 1_4:-33688273 None:intergenic
TCTTACTCCTTTCTATCTCT+CGG 0.448553 1_4:+33686417 Msa0160620:five_prime_UTR
GGATGAGGTTACCTTTGAAT+GGG 0.455125 1_4:+33688393 Msa0160620:CDS
GCCACTTCAATGCATGAATT+GGG 0.455426 1_4:-33686628 None:intergenic
TTCTTATGTGAATTAATATG+TGG 0.456754 1_4:+33686520 Msa0160620:five_prime_UTR
ATTGCAAATGACAATCTTGA+AGG 0.456999 1_4:+33688319 Msa0160620:CDS
AGAGGTAGAGGCAGCGGAAG+AGG 0.457632 1_4:+33688605 Msa0160620:three_prime_UTR
AGAATAGAAGGAGAAATTGA+AGG 0.460014 1_4:+33686874 Msa0160620:five_prime_UTR
GAAAGAAAATTGGGGAAAGA+AGG 0.470391 1_4:-33686601 None:intergenic
TTTGGGTTAAGATGAAGCTT+AGG 0.472756 1_4:+33686683 Msa0160620:five_prime_UTR
AACAAACCCAAAGGATTTCA+AGG 0.473412 1_4:+33688670 Msa0160620:three_prime_UTR
GCGTGTTCGTGCATTTGAAA+CGG 0.477104 1_4:+33687847 Msa0160620:five_prime_UTR
CACGTGTGATTGATTCATCT+TGG 0.478020 1_4:-33688293 None:intergenic
GTGCGGTTGTTTAGAGGCCG+TGG 0.478603 1_4:+33688745 Msa0160620:three_prime_UTR
AATATCTTTATCCCATTCAA+AGG 0.483583 1_4:-33688404 None:intergenic
ATACGGTCCTTTCTTGGATG+AGG 0.503815 1_4:+33688378 Msa0160620:CDS
AAAGATTGATGAAGTTCAAA+GGG 0.506733 1_4:-33686464 None:intergenic
GTAGGACTGTAGGTTCGGTT+GGG 0.513758 1_4:-33686577 None:intergenic
AATTTATTATCTGGTAGCAT+AGG 0.515942 1_4:+33688718 Msa0160620:three_prime_UTR
GAATAGAAGGAGAAATTGAA+GGG 0.517096 1_4:+33686875 Msa0160620:five_prime_UTR
TGTAGGACTGTAGGTTCGGT+TGG 0.518008 1_4:-33686578 None:intergenic
CAAAGATTGATGAAGTTCAA+AGG 0.524329 1_4:-33686465 None:intergenic
TCAAAGCTCTTGTTCATCAA+TGG 0.524483 1_4:+33686899 Msa0160620:five_prime_UTR
ACATTCTGTAATTGCTGCGT+AGG 0.525668 1_4:+33687883 Msa0160620:five_prime_UTR
TGATTTAGTTGGAGAAGTTG+AGG 0.526516 1_4:+33688351 Msa0160620:CDS
TTAGGTAGAGGTAGAGGCAG+CGG 0.532621 1_4:+33688599 Msa0160620:intron
GTGATTGATTCATCTTGGTA+TGG 0.533037 1_4:-33688288 None:intergenic
AGGACCGAGGCTTCTTGGCA+GGG 0.533687 1_4:+33688021 Msa0160620:five_prime_UTR
GGGCTATGGTGGCTTTGGAA+TGG 0.534025 1_4:-33686444 None:intergenic
TGAATGTTAGGTAGAGGTAG+AGG 0.536547 1_4:+33688593 Msa0160620:intron
GTGTTCCAGATTGTTTAGGC+AGG 0.537026 1_4:+33687993 Msa0160620:intron
ATACTTGAATTATTGAGCGT+TGG 0.537283 1_4:+33686835 Msa0160620:five_prime_UTR
AAGGTGTAGGACTGTAGGTT+CGG 0.544407 1_4:-33686582 None:intergenic
TGGATGGCCGAGAGATAGAA+AGG 0.550376 1_4:-33686424 None:intergenic
GAAAGAAGGTGTAGGACTGT+AGG 0.562934 1_4:-33686587 None:intergenic
AAGGTAACCTCATCCAAGAA+AGG 0.563434 1_4:-33688385 None:intergenic
TGATGAAGTTCAAAGGGCTA+TGG 0.564897 1_4:-33686458 None:intergenic
TATGGTGGCTTTGGAATGGA+TGG 0.568458 1_4:-33686440 None:intergenic
TCCCAATTCATGCATTGAAG+TGG 0.569900 1_4:+33686627 Msa0160620:five_prime_UTR
TGAAGTTCAAAGGGCTATGG+TGG 0.575476 1_4:-33686455 None:intergenic
TCAACCCTGCCAAGAAGCCT+CGG 0.575981 1_4:-33688025 None:intergenic
ATTAATTCACATAAGAAATG+AGG 0.577524 1_4:-33686514 None:intergenic
AATGGTTGTTTGTTATGTTG+CGG 0.583109 1_4:+33688880 Msa0160620:three_prime_UTR
CAATTCATGCATTGAAGTGG+CGG 0.588813 1_4:+33686630 Msa0160620:five_prime_UTR
AACTTTAGAAGATAGCAAGC+CGG 0.591008 1_4:+33688254 Msa0160620:five_prime_UTR
AGTAGAGAAATTGAAACAAG+AGG 0.594700 1_4:-33686661 None:intergenic
AAGCTCTTGTTCATCAATGG+TGG 0.595522 1_4:+33686902 Msa0160620:five_prime_UTR
TGATGAAACCTATTCTGACT+AGG 0.601466 1_4:+33688474 Msa0160620:CDS
AGAGAACTCACCATATCAAG+TGG 0.605853 1_4:-33686932 None:intergenic
TACTTCACAAAGTATCCTGA+AGG 0.606436 1_4:+33688180 Msa0160620:five_prime_UTR
TCATGCATTGAAGTGGCGGA+AGG 0.613551 1_4:+33686634 Msa0160620:five_prime_UTR
GTTGGCTGTGATTAAGACCA+AGG 0.613774 1_4:+33688215 Msa0160620:five_prime_UTR
GGAGAAGTTGAGGAAGCATA+CGG 0.617796 1_4:+33688361 Msa0160620:CDS
GAGAACTCACCATATCAAGT+GGG 0.622028 1_4:-33686931 None:intergenic
AGAGATAGAAAGGAGTAAGA+GGG 0.624355 1_4:-33686414 None:intergenic
TGGCAGGGTTGACGATTCAG+GGG 0.627660 1_4:+33688036 Msa0160620:five_prime_UTR
TGGGATAAGAAAGAAAATTG+GGG 0.635478 1_4:-33686609 None:intergenic
CTGGTAGCATAGGTTTCGTG+CGG 0.636336 1_4:+33688728 Msa0160620:three_prime_UTR
GGTTCGGTTGGGACTAGGAG+AGG 0.638556 1_4:-33686566 None:intergenic
ATCTTCTCAAACAAACCCAA+AGG 0.644202 1_4:+33688661 Msa0160620:three_prime_UTR
TAGGCAGGCAGTTAGGACCG+AGG 0.670686 1_4:+33688008 Msa0160620:five_prime_UTR
AGAACTCACCATATCAAGTG+GGG 0.690846 1_4:-33686930 None:intergenic
AAATTGGGGAAAGAAGGTGT+AGG 0.709182 1_4:-33686595 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATATCATATAAAGAAAAT+AGG - chr1_4:33688998-33689017 None:intergenic 15.0%
!!! TAAGATTACATGTATCATTT+TGG - chr1_4:33686491-33686510 None:intergenic 20.0%
!! ATTAATTCACATAAGAAATG+AGG - chr1_4:33686517-33686536 None:intergenic 20.0%
!! TTCTTATGTGAATTAATATG+TGG + chr1_4:33686520-33686539 Msa0160620:five_prime_UTR 20.0%
!! TCAACTCCTTAATTAATTAA+TGG + chr1_4:33686782-33686801 Msa0160620:intron 20.0%
!! ATGCAACCATTAATTAATTA+AGG - chr1_4:33686791-33686810 None:intergenic 20.0%
!! AGATTAATTCATGAAACAAA+TGG + chr1_4:33687065-33687084 Msa0160620:intron 20.0%
!!! AAATTTTGTCTATTTTCCAA+TGG - chr1_4:33687259-33687278 None:intergenic 20.0%
!! TCAAAATAACTTAAAAGTCT+TGG - chr1_4:33687572-33687591 None:intergenic 20.0%
!!! CAAGGTATTTTTATGATATA+AGG + chr1_4:33688688-33688707 Msa0160620:three_prime_UTR 20.0%
!! GGTTATTTACATGTAAAATA+TGG + chr1_4:33688946-33688965 Msa0160620:three_prime_UTR 20.0%
! AAAGATTGATGAAGTTCAAA+GGG - chr1_4:33686467-33686486 None:intergenic 25.0%
! TTGGGATAAGAAAGAAAATT+GGG - chr1_4:33686613-33686632 None:intergenic 25.0%
! ATTGGGATAAGAAAGAAAAT+TGG - chr1_4:33686614-33686633 None:intergenic 25.0%
! TTGTTTCAATTTCTCTACTT+TGG + chr1_4:33686665-33686684 Msa0160620:five_prime_UTR 25.0%
! TGTTTCAATTTCTCTACTTT+GGG + chr1_4:33686666-33686685 Msa0160620:five_prime_UTR 25.0%
! TTAATTAAGGAGTTGAAAGT+GGG - chr1_4:33686778-33686797 None:intergenic 25.0%
! ATTAATTAAGGAGTTGAAAG+TGG - chr1_4:33686779-33686798 None:intergenic 25.0%
! AATTAATTAATGGTTGCATG+TGG + chr1_4:33686792-33686811 Msa0160620:intron 25.0%
!!! TTAGAGTTGAATTTGTTGTT+TGG + chr1_4:33686976-33686995 Msa0160620:intron 25.0%
! ATTAATCTTGCACATAACAT+GGG - chr1_4:33687053-33687072 None:intergenic 25.0%
! AATTAATCTTGCACATAACA+TGG - chr1_4:33687054-33687073 None:intergenic 25.0%
! TTTAACGATGATAATCCATT+TGG - chr1_4:33687122-33687141 None:intergenic 25.0%
! ATCATCGTTAAAAGTGATTA+TGG + chr1_4:33687130-33687149 Msa0160620:intron 25.0%
! AATGTCAGTTAAAATTCGTA+TGG + chr1_4:33687170-33687189 Msa0160620:intron 25.0%
!! GACAGAAATTAACTTGAAAT+AGG + chr1_4:33687450-33687469 Msa0160620:intron 25.0%
! CTACTTGTAAATGTAACTTT+CGG + chr1_4:33687519-33687538 Msa0160620:intron 25.0%
! TTTATTACAAATTTCTGCGT+AGG + chr1_4:33687761-33687780 Msa0160620:intron 25.0%
! TTGAACATCAAAGGAAAAAA+AGG - chr1_4:33687933-33687952 None:intergenic 25.0%
! ACCATTAATGTAAAAATCCA+GGG - chr1_4:33688114-33688133 None:intergenic 25.0%
!!! TTGTGAAGTATACTTGTTTT+TGG - chr1_4:33688170-33688189 None:intergenic 25.0%
! AATATCTTTATCCCATTCAA+AGG - chr1_4:33688407-33688426 None:intergenic 25.0%
! ATAAATTACCTAGTCAGAAT+AGG - chr1_4:33688485-33688504 None:intergenic 25.0%
! TTGTATTGTTCATGAATGTT+AGG + chr1_4:33688581-33688600 Msa0160620:intron 25.0%
! AAAATACCTTGAAATCCTTT+GGG - chr1_4:33688679-33688698 None:intergenic 25.0%
! AAAAATACCTTGAAATCCTT+TGG - chr1_4:33688680-33688699 None:intergenic 25.0%
! GGTGCTTAAAATTTATTATC+TGG + chr1_4:33688709-33688728 Msa0160620:three_prime_UTR 25.0%
! AATTTATTATCTGGTAGCAT+AGG + chr1_4:33688718-33688737 Msa0160620:three_prime_UTR 25.0%
!!! AACACTTTTTGTTTCAGTTT+TGG + chr1_4:33688769-33688788 Msa0160620:three_prime_UTR 25.0%
!!! GTGTTTTTGACTTTGTTTAA+TGG + chr1_4:33688862-33688881 Msa0160620:three_prime_UTR 25.0%
!! TAACCATATTTTGCCATATA+AGG - chr1_4:33688931-33688950 None:intergenic 25.0%
CAAAGATTGATGAAGTTCAA+AGG - chr1_4:33686468-33686487 None:intergenic 30.0%
TGGGATAAGAAAGAAAATTG+GGG - chr1_4:33686612-33686631 None:intergenic 30.0%
AGTAGAGAAATTGAAACAAG+AGG - chr1_4:33686664-33686683 None:intergenic 30.0%
ATACTTGAATTATTGAGCGT+TGG + chr1_4:33686835-33686854 Msa0160620:five_prime_UTR 30.0%
AGAATAGAAGGAGAAATTGA+AGG + chr1_4:33686874-33686893 Msa0160620:five_prime_UTR 30.0%
GAATAGAAGGAGAAATTGAA+GGG + chr1_4:33686875-33686894 Msa0160620:five_prime_UTR 30.0%
!! AATTTGTTGTTTGGAGGTAA+TGG + chr1_4:33686985-33687004 Msa0160620:intron 30.0%
!! ATTTGTTGTTTGGAGGTAAT+GGG + chr1_4:33686986-33687005 Msa0160620:intron 30.0%
!! TCATGAAACAAATGGTTTTG+AGG + chr1_4:33687073-33687092 Msa0160620:intron 30.0%
TCGTTAAAAGTGATTATGGA+TGG + chr1_4:33687134-33687153 Msa0160620:intron 30.0%
! TACGTGATTCATTTTGTAGT+GGG + chr1_4:33687226-33687245 Msa0160620:intron 30.0%
ATTTAACACGAAATTGCCTT+TGG + chr1_4:33687275-33687294 Msa0160620:intron 30.0%
ACGAAATTAAGTTCTTCCAA+AGG - chr1_4:33687294-33687313 None:intergenic 30.0%
TCTATGTTCATACATCACTT+TGG + chr1_4:33687401-33687420 Msa0160620:intron 30.0%
AATTTCTGTCTTCTTTCTCA+TGG - chr1_4:33687440-33687459 None:intergenic 30.0%
TGAGTAGTCTAAGAATACAA+AGG + chr1_4:33687598-33687617 Msa0160620:intron 30.0%
AATTTCTGCGTAGGAATATT+CGG + chr1_4:33687770-33687789 Msa0160620:intron 30.0%
! CAATCTGGAACACAAATAAA+AGG - chr1_4:33687986-33688005 None:intergenic 30.0%
ATTTGTGTTCCAGATTGTTT+AGG + chr1_4:33687989-33688008 Msa0160620:intron 30.0%
!!! GTTTTATTTTATACCAACCC+TGG + chr1_4:33688094-33688113 Msa0160620:five_prime_UTR 30.0%
TTAATGTAAAAATCCAGGGT+TGG - chr1_4:33688110-33688129 None:intergenic 30.0%
! ACCCTGGATTTTTACATTAA+TGG + chr1_4:33688110-33688129 Msa0160620:five_prime_UTR 30.0%
CACCATTAATGTAAAAATCC+AGG - chr1_4:33688115-33688134 None:intergenic 30.0%
ATCCTGAAGGAAAAAAAGAT+TGG + chr1_4:33688193-33688212 Msa0160620:five_prime_UTR 30.0%
!!! AACCAATCTTTTTTTCCTTC+AGG - chr1_4:33688198-33688217 None:intergenic 30.0%
TGAAGGAAAAAAAGATTGGT+TGG + chr1_4:33688197-33688216 Msa0160620:five_prime_UTR 30.0%
ATTGCAAATGACAATCTTGA+AGG + chr1_4:33688319-33688338 Msa0160620:CDS 30.0%
ATCATCATCATCATCTTCTT+CGG - chr1_4:33688452-33688471 None:intergenic 30.0%
TAAGTCATGCAAGAATAAGA+GGG - chr1_4:33688519-33688538 None:intergenic 30.0%
ATAAGTCATGCAAGAATAAG+AGG - chr1_4:33688520-33688539 None:intergenic 30.0%
!! CTTTTTGTTTCAGTTTTGGT+TGG + chr1_4:33688773-33688792 Msa0160620:three_prime_UTR 30.0%
!! AATGGTTGTTTGTTATGTTG+CGG + chr1_4:33688880-33688899 Msa0160620:three_prime_UTR 30.0%
! CATATTTTGCCATATAAGGT+AGG - chr1_4:33688927-33688946 None:intergenic 30.0%
CTACCTTATATGGCAAAATA+TGG + chr1_4:33688925-33688944 Msa0160620:three_prime_UTR 30.0%
TAGAGAAAAACAATTGCAAC+TGG - chr1_4:33688973-33688992 None:intergenic 30.0%
AGAGATAGAAAGGAGTAAGA+GGG - chr1_4:33686417-33686436 None:intergenic 35.0%
TCTTACTCCTTTCTATCTCT+CGG + chr1_4:33686417-33686436 Msa0160620:five_prime_UTR 35.0%
GAAAGAAAATTGGGGAAAGA+AGG - chr1_4:33686604-33686623 None:intergenic 35.0%
! TTTGGGTTAAGATGAAGCTT+AGG + chr1_4:33686683-33686702 Msa0160620:five_prime_UTR 35.0%
GCAAGAACAAGAAGAATAGA+AGG + chr1_4:33686862-33686881 Msa0160620:five_prime_UTR 35.0%
TCAAAGCTCTTGTTCATCAA+TGG + chr1_4:33686899-33686918 Msa0160620:five_prime_UTR 35.0%
!! GAGTTGAATTTGTTGTTTGG+AGG + chr1_4:33686979-33686998 Msa0160620:intron 35.0%
CTACAAAATGAATCACGTAC+AGG - chr1_4:33687225-33687244 None:intergenic 35.0%
! GTACGTGATTCATTTTGTAG+TGG + chr1_4:33687225-33687244 Msa0160620:intron 35.0%
!! ACTTGAAATAGGCTTTGAGA+TGG + chr1_4:33687461-33687480 Msa0160620:intron 35.0%
AATTGCTGCGTAGGAATATT+CGG + chr1_4:33687892-33687911 Msa0160620:five_prime_UTR 35.0%
! AAGCACAAGTTGAACATCAA+AGG - chr1_4:33687942-33687961 None:intergenic 35.0%
TGGAACACAAATAAAAGGAG+TGG - chr1_4:33687981-33688000 None:intergenic 35.0%
!! ATTAATGGTGCTGCAAAGTT+TGG + chr1_4:33688125-33688144 Msa0160620:five_prime_UTR 35.0%
! TACTTCACAAAGTATCCTGA+AGG + chr1_4:33688180-33688199 Msa0160620:five_prime_UTR 35.0%
! AACTTTAGAAGATAGCAAGC+CGG + chr1_4:33688254-33688273 Msa0160620:five_prime_UTR 35.0%
! GTGATTGATTCATCTTGGTA+TGG - chr1_4:33688291-33688310 None:intergenic 35.0%
!! GGAAGTCTTGTTGATTTAGT+TGG + chr1_4:33688340-33688359 Msa0160620:CDS 35.0%
TGATTTAGTTGGAGAAGTTG+AGG + chr1_4:33688351-33688370 Msa0160620:CDS 35.0%
TGATGAAACCTATTCTGACT+AGG + chr1_4:33688474-33688493 Msa0160620:CDS 35.0%
TGTTCATGAATGTTAGGTAG+AGG + chr1_4:33688587-33688606 Msa0160620:intron 35.0%
ATCTTCTCAAACAAACCCAA+AGG + chr1_4:33688661-33688680 Msa0160620:three_prime_UTR 35.0%
AACAAACCCAAAGGATTTCA+AGG + chr1_4:33688670-33688689 Msa0160620:three_prime_UTR 35.0%
!! TGAAACAAAAAGTGTTGCCA+CGG - chr1_4:33688765-33688784 None:intergenic 35.0%
! ATTTTGCCATATAAGGTAGG+CGG - chr1_4:33688924-33688943 None:intergenic 35.0%
GAGAGATAGAAAGGAGTAAG+AGG - chr1_4:33686418-33686437 None:intergenic 40.0%
TGATGAAGTTCAAAGGGCTA+TGG - chr1_4:33686461-33686480 None:intergenic 40.0%
! AAATTGGGGAAAGAAGGTGT+AGG - chr1_4:33686598-33686617 None:intergenic 40.0%
TCCCAATTCATGCATTGAAG+TGG + chr1_4:33686627-33686646 Msa0160620:five_prime_UTR 40.0%
GCCACTTCAATGCATGAATT+GGG - chr1_4:33686631-33686650 None:intergenic 40.0%
CAATTCATGCATTGAAGTGG+CGG + chr1_4:33686630-33686649 Msa0160620:five_prime_UTR 40.0%
AAGCTCTTGTTCATCAATGG+TGG + chr1_4:33686902-33686921 Msa0160620:five_prime_UTR 40.0%
AGAACTCACCATATCAAGTG+GGG - chr1_4:33686933-33686952 None:intergenic 40.0%
GAGAACTCACCATATCAAGT+GGG - chr1_4:33686934-33686953 None:intergenic 40.0%
AGAGAACTCACCATATCAAG+TGG - chr1_4:33686935-33686954 None:intergenic 40.0%
!! TTGTTTGGAGGTAATGGGTT+TGG + chr1_4:33686991-33687010 Msa0160620:intron 40.0%
!! TGTTTGGAGGTAATGGGTTT+GGG + chr1_4:33686992-33687011 Msa0160620:intron 40.0%
GGGACATGTTTGAGAAAAGT+AGG + chr1_4:33687012-33687031 Msa0160620:intron 40.0%
!!! AAATGGTTTTGAGGCTGAGA+CGG + chr1_4:33687082-33687101 Msa0160620:intron 40.0%
GTGAAGCAATGACATCCAAA+TGG + chr1_4:33687104-33687123 Msa0160620:intron 40.0%
AATTCGTATGGACCTGTTGT+CGG + chr1_4:33687182-33687201 Msa0160620:intron 40.0%
! ATGACAGTTTTACCGACAAC+AGG - chr1_4:33687197-33687216 None:intergenic 40.0%
! TGTAGTGGGAATTCAACCAT+TGG + chr1_4:33687240-33687259 Msa0160620:intron 40.0%
TCGGCAAGCAAATTCGAATT+TGG + chr1_4:33687538-33687557 Msa0160620:intron 40.0%
CGGCAAGCAAATTCGAATTT+GGG + chr1_4:33687539-33687558 Msa0160620:intron 40.0%
! TGTTCTTGCGTTTTCGATCT+TGG + chr1_4:33687820-33687839 Msa0160620:five_prime_UTR 40.0%
ACATTCTGTAATTGCTGCGT+AGG + chr1_4:33687883-33687902 Msa0160620:five_prime_UTR 40.0%
TGGTATGGAACTTCTTTCTC+CGG - chr1_4:33688276-33688295 None:intergenic 40.0%
! CACGTGTGATTGATTCATCT+TGG - chr1_4:33688296-33688315 None:intergenic 40.0%
AAGGTAACCTCATCCAAGAA+AGG - chr1_4:33688388-33688407 None:intergenic 40.0%
TGGATGAGGTTACCTTTGAA+TGG + chr1_4:33688392-33688411 Msa0160620:CDS 40.0%
GGATGAGGTTACCTTTGAAT+GGG + chr1_4:33688393-33688412 Msa0160620:CDS 40.0%
TGAATGTTAGGTAGAGGTAG+AGG + chr1_4:33688593-33688612 Msa0160620:intron 40.0%
!! TATGGTGGCTTTGGAATGGA+TGG - chr1_4:33686443-33686462 None:intergenic 45.0%
TGAAGTTCAAAGGGCTATGG+TGG - chr1_4:33686458-33686477 None:intergenic 45.0%
AAGGTGTAGGACTGTAGGTT+CGG - chr1_4:33686585-33686604 None:intergenic 45.0%
! GAAAGAAGGTGTAGGACTGT+AGG - chr1_4:33686590-33686609 None:intergenic 45.0%
CGCCACTTCAATGCATGAAT+TGG - chr1_4:33686632-33686651 None:intergenic 45.0%
! AGGTTTCGGTGCAAGAAGAA+TGG - chr1_4:33686742-33686761 None:intergenic 45.0%
!! AGTTGAAAGTGGGCTGAACA+GGG - chr1_4:33686768-33686787 None:intergenic 45.0%
! GCATGTGGATTTTCTTGCTC+AGG + chr1_4:33686807-33686826 Msa0160620:intron 45.0%
TGGTTCATCCCCACTTGATA+TGG + chr1_4:33686922-33686941 Msa0160620:five_prime_UTR 45.0%
GCGTGTTCGTGCATTTGAAA+CGG + chr1_4:33687847-33687866 Msa0160620:five_prime_UTR 45.0%
! CGTGTTCGTGCATTTGAAAC+GGG + chr1_4:33687848-33687867 Msa0160620:five_prime_UTR 45.0%
GTGTTCCAGATTGTTTAGGC+AGG + chr1_4:33687993-33688012 Msa0160620:intron 45.0%
AACTGCCTGCCTAAACAATC+TGG - chr1_4:33688001-33688020 None:intergenic 45.0%
GATTGTTTAGGCAGGCAGTT+AGG + chr1_4:33688001-33688020 Msa0160620:intron 45.0%
GTTGGCTGTGATTAAGACCA+AGG + chr1_4:33688215-33688234 Msa0160620:five_prime_UTR 45.0%
TTGAATTGTGCTTCTCGCCT+TGG - chr1_4:33688235-33688254 None:intergenic 45.0%
! GGAGAAGTTGAGGAAGCATA+CGG + chr1_4:33688361-33688380 Msa0160620:CDS 45.0%
ATACGGTCCTTTCTTGGATG+AGG + chr1_4:33688378-33688397 Msa0160620:CDS 45.0%
!!! TGCGGCCAGTTTGTTGTTTT+TGG + chr1_4:33688898-33688917 Msa0160620:three_prime_UTR 45.0%
! TTTTGGCCGCCTACCTTATA+TGG + chr1_4:33688915-33688934 Msa0160620:three_prime_UTR 45.0%
TGGATGGCCGAGAGATAGAA+AGG - chr1_4:33686427-33686446 None:intergenic 50.0%
TCAAAGGGCTATGGTGGCTT+TGG - chr1_4:33686452-33686471 None:intergenic 50.0%
! GTAGGACTGTAGGTTCGGTT+GGG - chr1_4:33686580-33686599 None:intergenic 50.0%
! TGTAGGACTGTAGGTTCGGT+TGG - chr1_4:33686581-33686600 None:intergenic 50.0%
! TCATGCATTGAAGTGGCGGA+AGG + chr1_4:33686634-33686653 Msa0160620:five_prime_UTR 50.0%
GCTGAACAGGGGTTAGGTTT+CGG - chr1_4:33686756-33686775 None:intergenic 50.0%
!! GTTGAAAGTGGGCTGAACAG+GGG - chr1_4:33686767-33686786 None:intergenic 50.0%
!! GAGTTGAAAGTGGGCTGAAC+AGG - chr1_4:33686769-33686788 None:intergenic 50.0%
TTGGCAGGGTTGACGATTCA+GGG + chr1_4:33688035-33688054 Msa0160620:five_prime_UTR 50.0%
! GGAAGCATACGGTCCTTTCT+TGG + chr1_4:33688372-33688391 Msa0160620:CDS 50.0%
TTAGGTAGAGGTAGAGGCAG+CGG + chr1_4:33688599-33688618 Msa0160620:intron 50.0%
CTGGTAGCATAGGTTTCGTG+CGG + chr1_4:33688728-33688747 Msa0160620:three_prime_UTR 50.0%
GGTTTCGTGCGGTTGTTTAG+AGG + chr1_4:33688739-33688758 Msa0160620:three_prime_UTR 50.0%
GGCGGCCAAAAACAACAAAC+TGG - chr1_4:33688906-33688925 None:intergenic 50.0%
!! GGGCTATGGTGGCTTTGGAA+TGG - chr1_4:33686447-33686466 None:intergenic 55.0%
! CTGTAGGTTCGGTTGGGACT+AGG - chr1_4:33686574-33686593 None:intergenic 55.0%
!! AAGTGGGCTGAACAGGGGTT+AGG - chr1_4:33686762-33686781 None:intergenic 55.0%
CAGTTAGGACCGAGGCTTCT+TGG + chr1_4:33688016-33688035 Msa0160620:five_prime_UTR 55.0%
TCAACCCTGCCAAGAAGCCT+CGG - chr1_4:33688028-33688047 None:intergenic 55.0%
CTTGGCAGGGTTGACGATTC+AGG + chr1_4:33688034-33688053 Msa0160620:five_prime_UTR 55.0%
TGGCAGGGTTGACGATTCAG+GGG + chr1_4:33688036-33688055 Msa0160620:five_prime_UTR 55.0%
! GGTTCGGTTGGGACTAGGAG+AGG - chr1_4:33686569-33686588 None:intergenic 60.0%
TAGGCAGGCAGTTAGGACCG+AGG + chr1_4:33688008-33688027 Msa0160620:five_prime_UTR 60.0%
TAGGACCGAGGCTTCTTGGC+AGG + chr1_4:33688020-33688039 Msa0160620:five_prime_UTR 60.0%
AGGACCGAGGCTTCTTGGCA+GGG + chr1_4:33688021-33688040 Msa0160620:five_prime_UTR 60.0%
AGAGGTAGAGGCAGCGGAAG+AGG + chr1_4:33688605-33688624 Msa0160620:three_prime_UTR 60.0%
GTGCGGTTGTTTAGAGGCCG+TGG + chr1_4:33688745-33688764 Msa0160620:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 33686396 33689024 33686396 ID=Msa0160620;Name=Msa0160620
chr1_4 mRNA 33686396 33689024 33686396 ID=Msa0160620-mRNA-1;Parent=Msa0160620;Name=Msa0160620-mRNA-1;_AED=0.01;_eAED=0.01;_QI=851|1|1|1|0|0|5|422|65
chr1_4 exon 33686396 33686704 33686396 ID=Msa0160620-mRNA-1:exon:10439;Parent=Msa0160620-mRNA-1
chr1_4 exon 33686829 33686943 33686829 ID=Msa0160620-mRNA-1:exon:10440;Parent=Msa0160620-mRNA-1
chr1_4 exon 33687783 33687913 33687783 ID=Msa0160620-mRNA-1:exon:10441;Parent=Msa0160620-mRNA-1
chr1_4 exon 33688002 33688495 33688002 ID=Msa0160620-mRNA-1:exon:10442;Parent=Msa0160620-mRNA-1
chr1_4 exon 33688603 33689024 33688603 ID=Msa0160620-mRNA-1:exon:10443;Parent=Msa0160620-mRNA-1
chr1_4 five_prime_UTR 33686396 33686704 33686396 ID=Msa0160620-mRNA-1:five_prime_utr;Parent=Msa0160620-mRNA-1
chr1_4 five_prime_UTR 33686829 33686943 33686829 ID=Msa0160620-mRNA-1:five_prime_utr;Parent=Msa0160620-mRNA-1
chr1_4 five_prime_UTR 33687783 33687913 33687783 ID=Msa0160620-mRNA-1:five_prime_utr;Parent=Msa0160620-mRNA-1
chr1_4 five_prime_UTR 33688002 33688297 33688002 ID=Msa0160620-mRNA-1:five_prime_utr;Parent=Msa0160620-mRNA-1
chr1_4 CDS 33688298 33688495 33688298 ID=Msa0160620-mRNA-1:cds;Parent=Msa0160620-mRNA-1
chr1_4 three_prime_UTR 33688603 33689024 33688603 ID=Msa0160620-mRNA-1:three_prime_utr;Parent=Msa0160620-mRNA-1
Gene Sequence

>Msa0160620

ATGAATCAATCACACGTGACAATTGCAAATGACAATCTTGAAGGAAGTCTTGTTGATTTAGTTGGAGAAGTTGAGGAAGCATACGGTCCTTTCTTGGATGAGGTTACCTTTGAATGGGATAAAGATATTGAAGTAGAAGTAGAAGACAGTGCCGAAGAAGATGATGATGATGATGATGATGAAACCTATTCTGACTAG
Protein sequence

>Msa0160620

MNQSHVTIANDNLEGSLVDLVGEVEEAYGPFLDEVTFEWDKDIEVEVEDSAEEDDDDDDDETYSD*