AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1400810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1400810 MtrunA17_Chr4g0043351 87.097 155 17 1 16 167 8 162 2.40e-96 275
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 73 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATTACTTTAGAATTAATT+TGG 0.220352 tig0022477:+18822 Msa1400810:three_prime_UTR
TAGATCAAGCTAACCTTTCT+TGG 0.224166 tig0022477:+18638 Msa1400810:three_prime_UTR
GATGGAATTGAACCGCTTTC+AGG 0.230161 tig0022477:+18095 Msa1400810:CDS
TTCAAAGTTCAAATTCTTAG+AGG 0.303256 tig0022477:+18500 Msa1400810:CDS
AGTTGGGCCACGACTTATAA+TGG 0.308961 tig0022477:+18356 Msa1400810:CDS
ACGGGTACTAAGATGTGTTT+TGG 0.320299 tig0022477:-18144 None:intergenic
TTCCGACTATTCTCAAATAT+CGG 0.320685 tig0022477:+18327 Msa1400810:CDS
TTTCTTGGCTTGACCTTTGT+GGG 0.323305 tig0022477:+18653 Msa1400810:three_prime_UTR
AAGTAATATGTTTCTCTGAT+TGG 0.351267 tig0022477:-18808 None:intergenic
GCATCCCTCTCACCGGTTCC+AGG 0.356774 tig0022477:-18554 None:intergenic
CTTTCTTGGCTTGACCTTTG+TGG 0.363705 tig0022477:+18652 Msa1400810:three_prime_UTR
AGAGCATAGACTTTGGTGTT+AGG 0.364464 tig0022477:+18718 Msa1400810:three_prime_UTR
GACCTTTGTGGGATTGTTCA+AGG 0.364760 tig0022477:+18664 Msa1400810:three_prime_UTR
GAGAATAGTCGGAACCGATT+TGG 0.387243 tig0022477:-18318 None:intergenic
ACACATCTTAGTACCCGTTA+TGG 0.396282 tig0022477:+18149 Msa1400810:CDS
GAAGATAATTATTTGAAGCT+TGG 0.405476 tig0022477:+18428 Msa1400810:CDS
TCCGACTATTCTCAAATATC+GGG 0.408506 tig0022477:+18328 Msa1400810:CDS
GCCAACGCTATCAAACAGTT+TGG 0.414501 tig0022477:-18390 None:intergenic
AACTGTTTGATAGCGTTGGC+TGG 0.415821 tig0022477:+18393 Msa1400810:CDS
GAAAGGACCACATGAAAGTA+TGG 0.437117 tig0022477:-18122 None:intergenic
TCGGTGATGGTGGTTGGTGG+TGG 0.442258 tig0022477:-17736 None:intergenic
ATACAGAGGGAGAGTGATGA+TGG 0.448288 tig0022477:-17699 None:intergenic
CTTTACGAATTTCCTGGAAC+CGG 0.450094 tig0022477:+18542 Msa1400810:CDS
TGAAATTACCAATCCATAAC+GGG 0.462093 tig0022477:-18162 None:intergenic
CAAATATCGGGGTAAGAGTT+GGG 0.464634 tig0022477:+18340 Msa1400810:CDS
GATTACTAATCTATGACAAA+TGG 0.467072 tig0022477:-18578 None:intergenic
TCAAATATCGGGGTAAGAGT+TGG 0.468409 tig0022477:+18339 Msa1400810:CDS
TGAAGAACTTCCTTCCAAAT+CGG 0.469161 tig0022477:+18304 Msa1400810:CDS
GTTGGGCCACGACTTATAAT+GGG 0.482324 tig0022477:+18357 Msa1400810:CDS
TAAAGGTCGCCGGAAGGATG+TGG 0.497755 tig0022477:-17775 None:intergenic
TTGTCGGTGATGGTGGTTGG+TGG 0.500922 tig0022477:-17739 None:intergenic
ATGTCGTCTTCAAAATCAGC+TGG 0.502794 tig0022477:+17910 Msa1400810:five_prime_UTR
TCTTAGTACCCGTTATGGAT+TGG 0.505736 tig0022477:+18154 Msa1400810:CDS
ACCAAACTGTTTGATAGCGT+TGG 0.508522 tig0022477:+18389 Msa1400810:CDS
AATAGTGAAAAGTTATGTAC+TGG 0.509208 tig0022477:+18741 Msa1400810:three_prime_UTR
ATGAAATTACCAATCCATAA+CGG 0.511215 tig0022477:-18163 None:intergenic
CACAAAGGTCAAGCCAAGAA+AGG 0.514465 tig0022477:-18651 None:intergenic
CCCCGATATTTGAGAATAGT+CGG 0.515602 tig0022477:-18329 None:intergenic
CGAACAGATCCACATCCTTC+CGG 0.519644 tig0022477:+17766 Msa1400810:five_prime_UTR
CGGAAGGATGTGGATCTGTT+CGG 0.521075 tig0022477:-17765 None:intergenic
ACCTTCGACAAATATGCAAA+CGG 0.530039 tig0022477:+18073 Msa1400810:CDS
GTGTACATGAAAGTTAATCT+TGG 0.531460 tig0022477:-17969 None:intergenic
TAATGTGATATGAAATACTA+AGG 0.533146 tig0022477:-17946 None:intergenic
TGTTCGGAAGTTGTCGGTGA+TGG 0.534909 tig0022477:-17749 None:intergenic
ACTGTTTGATAGCGTTGGCT+GGG 0.539366 tig0022477:+18394 Msa1400810:CDS
GAAGGTGATGATAACGAGGC+TGG 0.539673 tig0022477:-18056 None:intergenic
TTACTTTAGAATTAATTTGG+TGG 0.543443 tig0022477:+18825 Msa1400810:three_prime_UTR
TCCGTTTGCATATTTGTCGA+AGG 0.549182 tig0022477:-18074 None:intergenic
GTTCTGCCCATTATAAGTCG+TGG 0.557426 tig0022477:-18363 None:intergenic
GGTTGGTGGTGGAAAAGAAA+AGG 0.558273 tig0022477:-17725 None:intergenic
AAGTTGTCGGTGATGGTGGT+TGG 0.562351 tig0022477:-17742 None:intergenic
AAAGAAAAGGAAAATACAGA+GGG 0.564283 tig0022477:-17712 None:intergenic
TCTTCAAAATCAGCTGGAAG+AGG 0.564849 tig0022477:+17916 Msa1400810:five_prime_UTR
AAATGGTGCATCCCTCTCAC+CGG 0.578086 tig0022477:-18561 None:intergenic
AGGAGTGCCATACTTTCATG+TGG 0.578309 tig0022477:+18115 Msa1400810:CDS
TCGACAAATATGCAAACGGA+TGG 0.583245 tig0022477:+18077 Msa1400810:CDS
GAGTAGCAGTCAAGTTGCTG+TGG 0.583739 tig0022477:+18028 Msa1400810:CDS
TCTTCACATATGCTGCTGCT+CGG 0.585483 tig0022477:-18287 None:intergenic
ATTTCCTGGAACCGGTGAGA+GGG 0.587927 tig0022477:+18550 Msa1400810:CDS
AGTAGCAGTCAAGTTGCTGT+GGG 0.603556 tig0022477:+18029 Msa1400810:CDS
GTAGGTTAGAGCATAGACTT+TGG 0.607501 tig0022477:+18711 Msa1400810:three_prime_UTR
TGGATCTGTTCGGAAGTTGT+CGG 0.611775 tig0022477:-17755 None:intergenic
TTAACTTTCATGTACACGGT+CGG 0.616082 tig0022477:+17975 Msa1400810:exon
TCGGAAGTTGTCGGTGATGG+TGG 0.619536 tig0022477:-17746 None:intergenic
TTTAAGTAAAGGTCGCCGGA+AGG 0.626587 tig0022477:-17781 None:intergenic
AAAAGAAAAGGAAAATACAG+AGG 0.630802 tig0022477:-17713 None:intergenic
AATTTCCTGGAACCGGTGAG+AGG 0.633829 tig0022477:+18549 Msa1400810:CDS
ATAGTCGGAACCGATTTGGA+AGG 0.643344 tig0022477:-18314 None:intergenic
AAGATTAACTTTCATGTACA+CGG 0.643809 tig0022477:+17971 Msa1400810:exon
AAGTATGGCACTCCTGAAAG+CGG 0.678549 tig0022477:-18107 None:intergenic
CCGACTATTCTCAAATATCG+GGG 0.681942 tig0022477:+18329 Msa1400810:CDS
AACCTTGAACAATCCCACAA+AGG 0.707964 tig0022477:-18666 None:intergenic
TGTCGAAGGTGATGATAACG+AGG 0.728967 tig0022477:-18060 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTAATTTTTTTTAAGTAA+AGG - tig0022477:17795-17814 None:intergenic 10.0%
!!! ATATTACTTTAGAATTAATT+TGG + tig0022477:18822-18841 Msa1400810:three_prime_UTR 10.0%
!!! ATGTAATTTTTTTTAAGTAA+AGG - tig0022477:17795-17814 None:intergenic 10.0%
!!! ATATTACTTTAGAATTAATT+TGG + tig0022477:18822-18841 Msa1400810:three_prime_UTR 10.0%
!!! TATCAAATTAGATTTTCAAA+AGG + tig0022477:17847-17866 Msa1400810:five_prime_UTR 15.0%
!!! TATCAAATTAGATTTTCAAA+AGG + tig0022477:17847-17866 Msa1400810:five_prime_UTR 15.0%
!! TAATGTGATATGAAATACTA+AGG - tig0022477:17949-17968 None:intergenic 20.0%
!! TTATAGATCAAACTAAATGA+AGG + tig0022477:18203-18222 Msa1400810:intron 20.0%
!! TAAATCTTGTATTGTATTGT+TGG + tig0022477:18254-18273 Msa1400810:intron 20.0%
!!! TTACTTTAGAATTAATTTGG+TGG + tig0022477:18825-18844 Msa1400810:three_prime_UTR 20.0%
!! TAATGTGATATGAAATACTA+AGG - tig0022477:17949-17968 None:intergenic 20.0%
!! TTATAGATCAAACTAAATGA+AGG + tig0022477:18203-18222 Msa1400810:intron 20.0%
!! TAAATCTTGTATTGTATTGT+TGG + tig0022477:18254-18273 Msa1400810:intron 20.0%
!!! TTACTTTAGAATTAATTTGG+TGG + tig0022477:18825-18844 Msa1400810:three_prime_UTR 20.0%
! AAAGAAAAGGAAAATACAGA+GGG - tig0022477:17715-17734 None:intergenic 25.0%
! AAAAGAAAAGGAAAATACAG+AGG - tig0022477:17716-17735 None:intergenic 25.0%
! AAGATTAACTTTCATGTACA+CGG + tig0022477:17971-17990 Msa1400810:exon 25.0%
! ATGAAATTACCAATCCATAA+CGG - tig0022477:18166-18185 None:intergenic 25.0%
!! ATCAAACTAAATGAAGGTTT+TGG + tig0022477:18209-18228 Msa1400810:intron 25.0%
! TATTGTATTGTTGGCATATA+GGG + tig0022477:18263-18282 Msa1400810:intron 25.0%
!! GAAGATAATTATTTGAAGCT+TGG + tig0022477:18428-18447 Msa1400810:CDS 25.0%
! TTCAAAGTTCAAATTCTTAG+AGG + tig0022477:18500-18519 Msa1400810:CDS 25.0%
! GATTACTAATCTATGACAAA+TGG - tig0022477:18581-18600 None:intergenic 25.0%
!! GATCTAGTTTCAAAAAGAAA+AGG - tig0022477:18624-18643 None:intergenic 25.0%
! AATAGTGAAAAGTTATGTAC+TGG + tig0022477:18741-18760 Msa1400810:three_prime_UTR 25.0%
! AAGTAATATGTTTCTCTGAT+TGG - tig0022477:18811-18830 None:intergenic 25.0%
! AAAGAAAAGGAAAATACAGA+GGG - tig0022477:17715-17734 None:intergenic 25.0%
! AAAAGAAAAGGAAAATACAG+AGG - tig0022477:17716-17735 None:intergenic 25.0%
! AAGATTAACTTTCATGTACA+CGG + tig0022477:17971-17990 Msa1400810:exon 25.0%
! ATGAAATTACCAATCCATAA+CGG - tig0022477:18166-18185 None:intergenic 25.0%
!! ATCAAACTAAATGAAGGTTT+TGG + tig0022477:18209-18228 Msa1400810:intron 25.0%
! TATTGTATTGTTGGCATATA+GGG + tig0022477:18263-18282 Msa1400810:intron 25.0%
!! GAAGATAATTATTTGAAGCT+TGG + tig0022477:18428-18447 Msa1400810:CDS 25.0%
! TTCAAAGTTCAAATTCTTAG+AGG + tig0022477:18500-18519 Msa1400810:CDS 25.0%
! GATTACTAATCTATGACAAA+TGG - tig0022477:18581-18600 None:intergenic 25.0%
!! GATCTAGTTTCAAAAAGAAA+AGG - tig0022477:18624-18643 None:intergenic 25.0%
! AATAGTGAAAAGTTATGTAC+TGG + tig0022477:18741-18760 Msa1400810:three_prime_UTR 25.0%
! AAGTAATATGTTTCTCTGAT+TGG - tig0022477:18811-18830 None:intergenic 25.0%
!!! TTTTTTTAAGTAAAGGTCGC+CGG - tig0022477:17788-17807 None:intergenic 30.0%
GTGTACATGAAAGTTAATCT+TGG - tig0022477:17972-17991 None:intergenic 30.0%
!!! TACTAAGATGTGTTTTGGAA+AGG - tig0022477:18142-18161 None:intergenic 30.0%
TGAAATTACCAATCCATAAC+GGG - tig0022477:18165-18184 None:intergenic 30.0%
!!! ACTAAATGAAGGTTTTGGAA+AGG + tig0022477:18214-18233 Msa1400810:intron 30.0%
GTATTGTATTGTTGGCATAT+AGG + tig0022477:18262-18281 Msa1400810:intron 30.0%
ATTGTATTGTTGGCATATAG+GGG + tig0022477:18264-18283 Msa1400810:intron 30.0%
TTCCGACTATTCTCAAATAT+CGG + tig0022477:18327-18346 Msa1400810:CDS 30.0%
GGAAATTCGTAAAGCAAAAA+TGG - tig0022477:18536-18555 None:intergenic 30.0%
! TTTTTGCTTTACGAATTTCC+TGG + tig0022477:18536-18555 Msa1400810:CDS 30.0%
!!! GATACTTTTGTTTTGTGAGT+AGG + tig0022477:18693-18712 Msa1400810:three_prime_UTR 30.0%
!!! TTTTTTTAAGTAAAGGTCGC+CGG - tig0022477:17788-17807 None:intergenic 30.0%
GTGTACATGAAAGTTAATCT+TGG - tig0022477:17972-17991 None:intergenic 30.0%
!!! TACTAAGATGTGTTTTGGAA+AGG - tig0022477:18142-18161 None:intergenic 30.0%
TGAAATTACCAATCCATAAC+GGG - tig0022477:18165-18184 None:intergenic 30.0%
!!! ACTAAATGAAGGTTTTGGAA+AGG + tig0022477:18214-18233 Msa1400810:intron 30.0%
GTATTGTATTGTTGGCATAT+AGG + tig0022477:18262-18281 Msa1400810:intron 30.0%
ATTGTATTGTTGGCATATAG+GGG + tig0022477:18264-18283 Msa1400810:intron 30.0%
TTCCGACTATTCTCAAATAT+CGG + tig0022477:18327-18346 Msa1400810:CDS 30.0%
GGAAATTCGTAAAGCAAAAA+TGG - tig0022477:18536-18555 None:intergenic 30.0%
! TTTTTGCTTTACGAATTTCC+TGG + tig0022477:18536-18555 Msa1400810:CDS 30.0%
!!! GATACTTTTGTTTTGTGAGT+AGG + tig0022477:18693-18712 Msa1400810:three_prime_UTR 30.0%
TTAACTTTCATGTACACGGT+CGG + tig0022477:17975-17994 Msa1400810:exon 35.0%
ACCTTCGACAAATATGCAAA+CGG + tig0022477:18073-18092 Msa1400810:CDS 35.0%
TGAAGAACTTCCTTCCAAAT+CGG + tig0022477:18304-18323 Msa1400810:CDS 35.0%
TCCGACTATTCTCAAATATC+GGG + tig0022477:18328-18347 Msa1400810:CDS 35.0%
TAGATCAAGCTAACCTTTCT+TGG + tig0022477:18638-18657 Msa1400810:three_prime_UTR 35.0%
TTAACTTTCATGTACACGGT+CGG + tig0022477:17975-17994 Msa1400810:exon 35.0%
ACCTTCGACAAATATGCAAA+CGG + tig0022477:18073-18092 Msa1400810:CDS 35.0%
TGAAGAACTTCCTTCCAAAT+CGG + tig0022477:18304-18323 Msa1400810:CDS 35.0%
TCCGACTATTCTCAAATATC+GGG + tig0022477:18328-18347 Msa1400810:CDS 35.0%
TAGATCAAGCTAACCTTTCT+TGG + tig0022477:18638-18657 Msa1400810:three_prime_UTR 35.0%
ATGTCGTCTTCAAAATCAGC+TGG + tig0022477:17910-17929 Msa1400810:five_prime_UTR 40.0%
TCTTCAAAATCAGCTGGAAG+AGG + tig0022477:17916-17935 Msa1400810:five_prime_UTR 40.0%
TCCGTTTGCATATTTGTCGA+AGG - tig0022477:18077-18096 None:intergenic 40.0%
TCGACAAATATGCAAACGGA+TGG + tig0022477:18077-18096 Msa1400810:CDS 40.0%
GAAAGGACCACATGAAAGTA+TGG - tig0022477:18125-18144 None:intergenic 40.0%
! ACGGGTACTAAGATGTGTTT+TGG - tig0022477:18147-18166 None:intergenic 40.0%
ACACATCTTAGTACCCGTTA+TGG + tig0022477:18149-18168 Msa1400810:CDS 40.0%
TCTTAGTACCCGTTATGGAT+TGG + tig0022477:18154-18173 Msa1400810:CDS 40.0%
CCCCGATATTTGAGAATAGT+CGG - tig0022477:18332-18351 None:intergenic 40.0%
CCGACTATTCTCAAATATCG+GGG + tig0022477:18329-18348 Msa1400810:CDS 40.0%
TCAAATATCGGGGTAAGAGT+TGG + tig0022477:18339-18358 Msa1400810:CDS 40.0%
CAAATATCGGGGTAAGAGTT+GGG + tig0022477:18340-18359 Msa1400810:CDS 40.0%
!! ACCAAACTGTTTGATAGCGT+TGG + tig0022477:18389-18408 Msa1400810:CDS 40.0%
GCTCATGAAAAACAGTGAAG+AGG + tig0022477:18469-18488 Msa1400810:CDS 40.0%
CTTTACGAATTTCCTGGAAC+CGG + tig0022477:18542-18561 Msa1400810:CDS 40.0%
! TTTCTTGGCTTGACCTTTGT+GGG + tig0022477:18653-18672 Msa1400810:three_prime_UTR 40.0%
AACCTTGAACAATCCCACAA+AGG - tig0022477:18669-18688 None:intergenic 40.0%
GTAGGTTAGAGCATAGACTT+TGG + tig0022477:18711-18730 Msa1400810:three_prime_UTR 40.0%
!! AGAGCATAGACTTTGGTGTT+AGG + tig0022477:18718-18737 Msa1400810:three_prime_UTR 40.0%
ATGTCGTCTTCAAAATCAGC+TGG + tig0022477:17910-17929 Msa1400810:five_prime_UTR 40.0%
TCTTCAAAATCAGCTGGAAG+AGG + tig0022477:17916-17935 Msa1400810:five_prime_UTR 40.0%
TCCGTTTGCATATTTGTCGA+AGG - tig0022477:18077-18096 None:intergenic 40.0%
TCGACAAATATGCAAACGGA+TGG + tig0022477:18077-18096 Msa1400810:CDS 40.0%
GAAAGGACCACATGAAAGTA+TGG - tig0022477:18125-18144 None:intergenic 40.0%
! ACGGGTACTAAGATGTGTTT+TGG - tig0022477:18147-18166 None:intergenic 40.0%
ACACATCTTAGTACCCGTTA+TGG + tig0022477:18149-18168 Msa1400810:CDS 40.0%
TCTTAGTACCCGTTATGGAT+TGG + tig0022477:18154-18173 Msa1400810:CDS 40.0%
CCCCGATATTTGAGAATAGT+CGG - tig0022477:18332-18351 None:intergenic 40.0%
CCGACTATTCTCAAATATCG+GGG + tig0022477:18329-18348 Msa1400810:CDS 40.0%
TCAAATATCGGGGTAAGAGT+TGG + tig0022477:18339-18358 Msa1400810:CDS 40.0%
CAAATATCGGGGTAAGAGTT+GGG + tig0022477:18340-18359 Msa1400810:CDS 40.0%
!! ACCAAACTGTTTGATAGCGT+TGG + tig0022477:18389-18408 Msa1400810:CDS 40.0%
GCTCATGAAAAACAGTGAAG+AGG + tig0022477:18469-18488 Msa1400810:CDS 40.0%
CTTTACGAATTTCCTGGAAC+CGG + tig0022477:18542-18561 Msa1400810:CDS 40.0%
! TTTCTTGGCTTGACCTTTGT+GGG + tig0022477:18653-18672 Msa1400810:three_prime_UTR 40.0%
AACCTTGAACAATCCCACAA+AGG - tig0022477:18669-18688 None:intergenic 40.0%
GTAGGTTAGAGCATAGACTT+TGG + tig0022477:18711-18730 Msa1400810:three_prime_UTR 40.0%
!! AGAGCATAGACTTTGGTGTT+AGG + tig0022477:18718-18737 Msa1400810:three_prime_UTR 40.0%
ATACAGAGGGAGAGTGATGA+TGG - tig0022477:17702-17721 None:intergenic 45.0%
!! GGTTGGTGGTGGAAAAGAAA+AGG - tig0022477:17728-17747 None:intergenic 45.0%
TGGATCTGTTCGGAAGTTGT+CGG - tig0022477:17758-17777 None:intergenic 45.0%
TTTAAGTAAAGGTCGCCGGA+AGG - tig0022477:17784-17803 None:intergenic 45.0%
! AGTAGCAGTCAAGTTGCTGT+GGG + tig0022477:18029-18048 Msa1400810:CDS 45.0%
! TGTCGAAGGTGATGATAACG+AGG - tig0022477:18063-18082 None:intergenic 45.0%
GATGGAATTGAACCGCTTTC+AGG + tig0022477:18095-18114 Msa1400810:CDS 45.0%
AAGTATGGCACTCCTGAAAG+CGG - tig0022477:18110-18129 None:intergenic 45.0%
!! AGGAGTGCCATACTTTCATG+TGG + tig0022477:18115-18134 Msa1400810:CDS 45.0%
TCTTCACATATGCTGCTGCT+CGG - tig0022477:18290-18309 None:intergenic 45.0%
!! ATAGTCGGAACCGATTTGGA+AGG - tig0022477:18317-18336 None:intergenic 45.0%
GAGAATAGTCGGAACCGATT+TGG - tig0022477:18321-18340 None:intergenic 45.0%
AGTTGGGCCACGACTTATAA+TGG + tig0022477:18356-18375 Msa1400810:CDS 45.0%
GTTGGGCCACGACTTATAAT+GGG + tig0022477:18357-18376 Msa1400810:CDS 45.0%
GTTCTGCCCATTATAAGTCG+TGG - tig0022477:18366-18385 None:intergenic 45.0%
GCCAACGCTATCAAACAGTT+TGG - tig0022477:18393-18412 None:intergenic 45.0%
!! AACTGTTTGATAGCGTTGGC+TGG + tig0022477:18393-18412 Msa1400810:CDS 45.0%
!! ACTGTTTGATAGCGTTGGCT+GGG + tig0022477:18394-18413 Msa1400810:CDS 45.0%
CACAAAGGTCAAGCCAAGAA+AGG - tig0022477:18654-18673 None:intergenic 45.0%
! CTTTCTTGGCTTGACCTTTG+TGG + tig0022477:18652-18671 Msa1400810:three_prime_UTR 45.0%
GACCTTTGTGGGATTGTTCA+AGG + tig0022477:18664-18683 Msa1400810:three_prime_UTR 45.0%
ATACAGAGGGAGAGTGATGA+TGG - tig0022477:17702-17721 None:intergenic 45.0%
!! GGTTGGTGGTGGAAAAGAAA+AGG - tig0022477:17728-17747 None:intergenic 45.0%
TGGATCTGTTCGGAAGTTGT+CGG - tig0022477:17758-17777 None:intergenic 45.0%
TTTAAGTAAAGGTCGCCGGA+AGG - tig0022477:17784-17803 None:intergenic 45.0%
! AGTAGCAGTCAAGTTGCTGT+GGG + tig0022477:18029-18048 Msa1400810:CDS 45.0%
! TGTCGAAGGTGATGATAACG+AGG - tig0022477:18063-18082 None:intergenic 45.0%
GATGGAATTGAACCGCTTTC+AGG + tig0022477:18095-18114 Msa1400810:CDS 45.0%
AAGTATGGCACTCCTGAAAG+CGG - tig0022477:18110-18129 None:intergenic 45.0%
!! AGGAGTGCCATACTTTCATG+TGG + tig0022477:18115-18134 Msa1400810:CDS 45.0%
TCTTCACATATGCTGCTGCT+CGG - tig0022477:18290-18309 None:intergenic 45.0%
!! ATAGTCGGAACCGATTTGGA+AGG - tig0022477:18317-18336 None:intergenic 45.0%
GAGAATAGTCGGAACCGATT+TGG - tig0022477:18321-18340 None:intergenic 45.0%
AGTTGGGCCACGACTTATAA+TGG + tig0022477:18356-18375 Msa1400810:CDS 45.0%
GTTGGGCCACGACTTATAAT+GGG + tig0022477:18357-18376 Msa1400810:CDS 45.0%
GTTCTGCCCATTATAAGTCG+TGG - tig0022477:18366-18385 None:intergenic 45.0%
GCCAACGCTATCAAACAGTT+TGG - tig0022477:18393-18412 None:intergenic 45.0%
!! AACTGTTTGATAGCGTTGGC+TGG + tig0022477:18393-18412 Msa1400810:CDS 45.0%
!! ACTGTTTGATAGCGTTGGCT+GGG + tig0022477:18394-18413 Msa1400810:CDS 45.0%
CACAAAGGTCAAGCCAAGAA+AGG - tig0022477:18654-18673 None:intergenic 45.0%
! CTTTCTTGGCTTGACCTTTG+TGG + tig0022477:18652-18671 Msa1400810:three_prime_UTR 45.0%
GACCTTTGTGGGATTGTTCA+AGG + tig0022477:18664-18683 Msa1400810:three_prime_UTR 45.0%
!! AAGTTGTCGGTGATGGTGGT+TGG - tig0022477:17745-17764 None:intergenic 50.0%
TGTTCGGAAGTTGTCGGTGA+TGG - tig0022477:17752-17771 None:intergenic 50.0%
CGGAAGGATGTGGATCTGTT+CGG - tig0022477:17768-17787 None:intergenic 50.0%
CGAACAGATCCACATCCTTC+CGG + tig0022477:17766-17785 Msa1400810:five_prime_UTR 50.0%
! GAGTAGCAGTCAAGTTGCTG+TGG + tig0022477:18028-18047 Msa1400810:CDS 50.0%
! GAAGGTGATGATAACGAGGC+TGG - tig0022477:18059-18078 None:intergenic 50.0%
AATTTCCTGGAACCGGTGAG+AGG + tig0022477:18549-18568 Msa1400810:CDS 50.0%
ATTTCCTGGAACCGGTGAGA+GGG + tig0022477:18550-18569 Msa1400810:CDS 50.0%
AAATGGTGCATCCCTCTCAC+CGG - tig0022477:18564-18583 None:intergenic 50.0%
!! AAGTTGTCGGTGATGGTGGT+TGG - tig0022477:17745-17764 None:intergenic 50.0%
TGTTCGGAAGTTGTCGGTGA+TGG - tig0022477:17752-17771 None:intergenic 50.0%
CGGAAGGATGTGGATCTGTT+CGG - tig0022477:17768-17787 None:intergenic 50.0%
CGAACAGATCCACATCCTTC+CGG + tig0022477:17766-17785 Msa1400810:five_prime_UTR 50.0%
! GAGTAGCAGTCAAGTTGCTG+TGG + tig0022477:18028-18047 Msa1400810:CDS 50.0%
! GAAGGTGATGATAACGAGGC+TGG - tig0022477:18059-18078 None:intergenic 50.0%
AATTTCCTGGAACCGGTGAG+AGG + tig0022477:18549-18568 Msa1400810:CDS 50.0%
ATTTCCTGGAACCGGTGAGA+GGG + tig0022477:18550-18569 Msa1400810:CDS 50.0%
AAATGGTGCATCCCTCTCAC+CGG - tig0022477:18564-18583 None:intergenic 50.0%
!! TTGTCGGTGATGGTGGTTGG+TGG - tig0022477:17742-17761 None:intergenic 55.0%
! TCGGAAGTTGTCGGTGATGG+TGG - tig0022477:17749-17768 None:intergenic 55.0%
TAAAGGTCGCCGGAAGGATG+TGG - tig0022477:17778-17797 None:intergenic 55.0%
!! TTGTCGGTGATGGTGGTTGG+TGG - tig0022477:17742-17761 None:intergenic 55.0%
! TCGGAAGTTGTCGGTGATGG+TGG - tig0022477:17749-17768 None:intergenic 55.0%
TAAAGGTCGCCGGAAGGATG+TGG - tig0022477:17778-17797 None:intergenic 55.0%
!! TCGGTGATGGTGGTTGGTGG+TGG - tig0022477:17739-17758 None:intergenic 60.0%
!! TCGGTGATGGTGGTTGGTGG+TGG - tig0022477:17739-17758 None:intergenic 60.0%
!! GCATCCCTCTCACCGGTTCC+AGG - tig0022477:18557-18576 None:intergenic 65.0%
!! GCATCCCTCTCACCGGTTCC+AGG - tig0022477:18557-18576 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0022477 gene 17687 18858 17687 ID=Msa1400810;Name=Msa1400810
tig0022477 mRNA 17687 18858 17687 ID=Msa1400810-mRNA-1;Parent=Msa1400810;Name=Msa1400810-mRNA-1;_AED=0.05;_eAED=0.05;_QI=297|1|1|1|1|1|2|263|167
tig0022477 exon 17687 18175 17687 ID=Msa1400810-mRNA-1:exon:17934;Parent=Msa1400810-mRNA-1
tig0022477 exon 18284 18858 18284 ID=Msa1400810-mRNA-1:exon:17935;Parent=Msa1400810-mRNA-1
tig0022477 five_prime_UTR 17687 17983 17687 ID=Msa1400810-mRNA-1:five_prime_utr;Parent=Msa1400810-mRNA-1
tig0022477 CDS 17984 18175 17984 ID=Msa1400810-mRNA-1:cds;Parent=Msa1400810-mRNA-1
tig0022477 CDS 18284 18595 18284 ID=Msa1400810-mRNA-1:cds;Parent=Msa1400810-mRNA-1
tig0022477 three_prime_UTR 18596 18858 18596 ID=Msa1400810-mRNA-1:three_prime_utr;Parent=Msa1400810-mRNA-1
Gene Sequence

>Msa1400810

ATGTACACGGTCGGTCTATCACTAATTTCACATTTATTTTCAGTGAGTAGCAGTCAAGTTGCTGTGGGAAATCCAGCCTCGTTATCATCACCTTCGACAAATATGCAAACGGATGGAATTGAACCGCTTTCAGGAGTGCCATACTTTCATGTGGTCCTTTCCAAAACACATCTTAGTACCCGTTATGGATTGGGGCCGAGCAGCAGCATATGTGAAGAACTTCCTTCCAAATCGGTTCCGACTATTCTCAAATATCGGGGTAAGAGTTGGGCCACGACTTATAATGGGCAGAACAAAACCAAACTGTTTGATAGCGTTGGCTGGGAAAAATTTGCTGAAGATAATTATTTGAAGCTTGGAGACGCTTGTGTTTTTGAGCTCATGAAAAACAGTGAAGAGGAAATCGTCTTCAAAGTTCAAATTCTTAGAGGTGAAGAAGAGCCATTTTTGCTTTACGAATTTCCTGGAACCGGTGAGAGGGATGCACCATTTGTCATAGATTAG
Protein sequence

>Msa1400810

MYTVGLSLISHLFSVSSSQVAVGNPASLSSPSTNMQTDGIEPLSGVPYFHVVLSKTHLSTRYGLGPSSSICEELPSKSVPTILKYRGKSWATTYNGQNKTKLFDSVGWEKFAEDNYLKLGDACVFELMKNSEEEIVFKVQILRGEEEPFLLYEFPGTGERDAPFVID*