AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1413270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1413270 MtrunA17_Chr8g0383401 99.528 212 1 0 1 212 1 212 4.11e-161 443
Msa1413270 MtrunA17_Chr6g0467101 50.711 211 81 5 1 204 1 195 2.32e-62 192
Msa1413270 MtrunA17_Chr1g0201541 49.519 208 100 5 1 204 1 207 1.42e-58 183
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1413270 AT4G09830.1 51.402 214 71 6 1 204 1 191 1.37e-55 175
Msa1413270 AT5G64780.1 46.591 176 86 4 1 174 1 170 1.55e-43 144

Find 92 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTTCGGTGCGGTGCTTGC+CGG 0.312526 tig0024019:-48349 None:intergenic
TGATATAGTCTCTCACTTGC+AGG 0.320186 tig0024019:+49014 Msa1413270:CDS
ACGAATTTGAATCTCTGTTC+TGG 0.321939 tig0024019:-51253 None:intergenic
CTGCCCTGTGCTAAATGATA+TGG 0.346225 tig0024019:-51161 None:intergenic
CTTCAGATTTCTCAAGGCTT+AGG 0.349977 tig0024019:+51569 Msa1413270:three_prime_UTR
GGCTGAAGGCTGCGACGAAT+GGG 0.351898 tig0024019:-51140 None:intergenic
AGTTAGTTTGAATTGCTGTT+TGG 0.364182 tig0024019:-51004 None:intergenic
TAACTATGATCAAATCAGTA+TGG 0.368060 tig0024019:-51329 None:intergenic
GTTAGTTTGAATTGCTGTTT+GGG 0.374257 tig0024019:-51003 None:intergenic
GCATTTGGAAGTACTGTTGC+AGG 0.382751 tig0024019:+51050 Msa1413270:CDS
ATTGACTGCCTTGGAGAAAC+TGG 0.385558 tig0024019:-50969 None:intergenic
CGCCATATCTCCACCGATCA+AGG 0.389326 tig0024019:-48413 None:intergenic
ATACACCATTTCATTGTTAC+GGG 0.393219 tig0024019:-51222 None:intergenic
GGTTCGGTGCGGTGCTTGCC+GGG 0.397100 tig0024019:-48348 None:intergenic
TCCATACCGTCCAATGCATT+TGG 0.397895 tig0024019:+51035 Msa1413270:CDS
GCAGGACAGGGGATACGAAC+TGG 0.401130 tig0024019:+51068 Msa1413270:CDS
TTCCTTGATCGGTGGAGATA+TGG 0.407978 tig0024019:+48411 Msa1413270:exon
TCATGTTGGAAGCTTCAAAC+TGG 0.412934 tig0024019:-51466 None:intergenic
GATACACCATTTCATTGTTA+CGG 0.418071 tig0024019:-51223 None:intergenic
TGGCTGAAGGCTGCGACGAA+TGG 0.418206 tig0024019:-51141 None:intergenic
AATTAATATAATTGAAAGAT+GGG 0.418807 tig0024019:-48369 None:intergenic
TAATTAATATAATTGAAAGA+TGG 0.422990 tig0024019:-48370 None:intergenic
TCGAGTAATGTCATGTCATC+GGG 0.423559 tig0024019:+51191 Msa1413270:CDS
TGGAAGTACTGTTGCAGGAC+AGG 0.432969 tig0024019:+51055 Msa1413270:CDS
ATGTAGAATGAGCTTGAATA+TGG 0.441794 tig0024019:+50933 Msa1413270:intron
GAAGAGAAGACTGGAACAAT+GGG 0.443494 tig0024019:-48312 None:intergenic
ATTGGGTATTGTATATATAG+AGG 0.448947 tig0024019:+48278 Msa1413270:five_prime_UTR
CGGAACCCGTAACAATGAAA+TGG 0.453370 tig0024019:+51217 Msa1413270:CDS
TTCTGTTGAATTGACTGCCT+TGG 0.458544 tig0024019:-50978 None:intergenic
AGAAGAGAAGACTGGAACAA+TGG 0.461600 tig0024019:-48313 None:intergenic
AATCTATGTTCATAAATGTT+TGG 0.466154 tig0024019:+51386 Msa1413270:three_prime_UTR
AAGACTTTATGCTTATGTAA+TGG 0.479258 tig0024019:-51528 None:intergenic
TACTGAGTTCTTTACTTGAT+CGG 0.479541 tig0024019:-51096 None:intergenic
TACAAGAGCGTGGCGTTCAC+CGG 0.488524 tig0024019:-48569 None:intergenic
TCCAAATGCATTGGACGGTA+TGG 0.490213 tig0024019:-51036 None:intergenic
TCTTCTCTTACTTCTCTGCC+CGG 0.492447 tig0024019:+48330 Msa1413270:five_prime_UTR
ACCAATCCTCCTTCCTTGAT+CGG 0.496313 tig0024019:+48400 Msa1413270:five_prime_UTR
GTGGCGTTCACCGGCTTGAA+AGG 0.497863 tig0024019:-48560 None:intergenic
GAGATGAGAGAGGGAATTAG+CGG 0.501181 tig0024019:-48506 None:intergenic
ATATAATTGAAAGATGGGTT+CGG 0.502181 tig0024019:-48364 None:intergenic
GGAAGTACTGTTGCAGGACA+GGG 0.507937 tig0024019:+51056 Msa1413270:CDS
GTCCATGCAATCACTGGAGT+TGG 0.512023 tig0024019:-51276 None:intergenic
ACCGATCAAGGAAGGAGGAT+TGG 0.519506 tig0024019:-48401 None:intergenic
GAAGTAAGAGAAGAGAAGAC+TGG 0.523441 tig0024019:-48321 None:intergenic
GAATCTCTGTTCTGGCCACT+AGG 0.523458 tig0024019:-51245 None:intergenic
TTCGAGTAATGTCATGTCAT+CGG 0.525561 tig0024019:+51190 Msa1413270:CDS
GGAATTAGCGGTGGTGCAGA+AGG 0.533371 tig0024019:-48494 None:intergenic
TGCATTGGACGGTATGGATG+CGG 0.534195 tig0024019:-51030 None:intergenic
AACAAAGTATACTCTCATGT+TGG 0.542600 tig0024019:-51480 None:intergenic
CGTGGGTATAGAGATGAGAG+AGG 0.545674 tig0024019:-48516 None:intergenic
GGAGTCTCGTCTCGATGAAG+TGG 0.547540 tig0024019:+48453 Msa1413270:CDS
TATTTGCTTCAGATTTCTCA+AGG 0.553276 tig0024019:+51563 Msa1413270:three_prime_UTR
GTCCAACTCCAGTGATTGCA+TGG 0.554317 tig0024019:+51274 Msa1413270:CDS
TGATTTGATCATAGTTAGAG+TGG 0.557330 tig0024019:+51335 Msa1413270:exon
CAGCCATATCATTTAGCACA+GGG 0.558095 tig0024019:+51158 Msa1413270:CDS
ATGCATGTCCATGCAATCAC+TGG 0.561386 tig0024019:-51282 None:intergenic
CAGTATGGAAAATCATGACC+AGG 0.574411 tig0024019:-51314 None:intergenic
AACAGTACTTCCAAATGCAT+TGG 0.577353 tig0024019:-51045 None:intergenic
ATTAGCGGTGGTGCAGAAGG+TGG 0.579586 tig0024019:-48491 None:intergenic
AATAAGAGTGTGTTAATGTG+AGG 0.579886 tig0024019:-51714 None:intergenic
TCAGCCATATCATTTAGCAC+AGG 0.580324 tig0024019:+51157 Msa1413270:CDS
GGTGCAGAAGGTGGAGTACA+TGG 0.586968 tig0024019:-48482 None:intergenic
ATAAGAGTGTGTTAATGTGA+GGG 0.588113 tig0024019:-51713 None:intergenic
GACATGCATGCAGACAGTCC+TGG 0.588311 tig0024019:+51296 Msa1413270:CDS
CAGGACAGGGGATACGAACT+GGG 0.588579 tig0024019:+51069 Msa1413270:CDS
AGTGGGTAACAGTAAAGAGA+AGG 0.590826 tig0024019:+51353 Msa1413270:three_prime_UTR
AATGTCATGTCATCGGGGAA+CGG 0.595236 tig0024019:+51197 Msa1413270:CDS
GTGGGTATAGAGATGAGAGA+GGG 0.599340 tig0024019:-48515 None:intergenic
ATTCTTAGACAACAACAAGA+AGG 0.606741 tig0024019:+48973 Msa1413270:CDS
AATCCTCCTTCCTTGATCGG+TGG 0.614077 tig0024019:+48403 Msa1413270:five_prime_UTR
ATATCTCCACCGATCAAGGA+AGG 0.614630 tig0024019:-48409 None:intergenic
TGCAGGAGAAAATGTATCAG+TGG 0.615078 tig0024019:+48950 Msa1413270:intron
GATTTGATCATAGTTAGAGT+GGG 0.618081 tig0024019:+51336 Msa1413270:exon
AAGCATAAAGTCTTACTAGT+GGG 0.618970 tig0024019:+51537 Msa1413270:three_prime_UTR
ATGAGAGAGGGAATTAGCGG+TGG 0.626062 tig0024019:-48503 None:intergenic
TAAGCATAAAGTCTTACTAG+TGG 0.627894 tig0024019:+51536 Msa1413270:three_prime_UTR
TGAGCTTGAATATGGAGCAG+AGG 0.628224 tig0024019:+50941 Msa1413270:CDS
GCTGAAGGCTGCGACGAATG+GGG 0.632700 tig0024019:-51139 None:intergenic
AGGAATCACCAGTTTCTCCA+AGG 0.640475 tig0024019:+50961 Msa1413270:CDS
GAAGTACTGTTGCAGGACAG+GGG 0.643448 tig0024019:+51057 Msa1413270:CDS
AGCTTCAAACTGGAAAACAA+TGG 0.643885 tig0024019:-51456 None:intergenic
GTGCTAAATGATATGGCTGA+AGG 0.646201 tig0024019:-51154 None:intergenic
GGGTATTGTATATATAGAGG+AGG 0.658495 tig0024019:+48281 Msa1413270:five_prime_UTR
AATGAAATGGTGTATCCTAG+TGG 0.663062 tig0024019:+51230 Msa1413270:CDS
GTACTTCCAAATGCATTGGA+CGG 0.665522 tig0024019:-51041 None:intergenic
AGAGAAGACTGGAACAATGG+GGG 0.670119 tig0024019:-48310 None:intergenic
AAGAGAAGACTGGAACAATG+GGG 0.686005 tig0024019:-48311 None:intergenic
GAAGGAAGCATACAAGAGCG+TGG 0.686735 tig0024019:-48579 None:intergenic
TCTCCACCGATCAAGGAAGG+AGG 0.686793 tig0024019:-48406 None:intergenic
GATGACATGACATTACTCGA+AGG 0.723139 tig0024019:-51188 None:intergenic
CGAGTAATGTCATGTCATCG+GGG 0.731834 tig0024019:+51192 Msa1413270:CDS
ATTGAAAGATGGGTTCGGTG+CGG 0.732396 tig0024019:-48359 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATATAATTGAAAGAT+GGG - tig0024019:48372-48391 None:intergenic 10.0%
!! TAATTAATATAATTGAAAGA+TGG - tig0024019:48373-48392 None:intergenic 10.0%
!! AATAATTTGAAAATAAGAAA+GGG - tig0024019:48624-48643 None:intergenic 10.0%
!! TAATAATTTGAAAATAAGAA+AGG - tig0024019:48625-48644 None:intergenic 10.0%
!! AAATAATTAAAAGAAAAGAA+TGG - tig0024019:48686-48705 None:intergenic 10.0%
!! AATATTATAAACAAAATTAG+GGG - tig0024019:48856-48875 None:intergenic 10.0%
!! CAAATATTATAAACAAAATT+AGG - tig0024019:48858-48877 None:intergenic 10.0%
!!! ATTACAATATTTTTTTTTGT+CGG - tig0024019:51662-51681 None:intergenic 10.0%
!! ATAATTTGAAAATAAGAAAG+GGG - tig0024019:48623-48642 None:intergenic 15.0%
!! TGATTTAATATAAAATGCTT+TGG + tig0024019:48913-48932 Msa1413270:intron 15.0%
!!! AATAAGATATTTTATAACAC+AGG + tig0024019:49443-49462 Msa1413270:intron 15.0%
!! GTATATTAAAAGAATAATCT+TGG + tig0024019:50083-50102 Msa1413270:intron 15.0%
!!! TAAGCTTATATAATTTTATG+AGG - tig0024019:50199-50218 None:intergenic 15.0%
!!! ATTTTCATTATGAGTTTTAT+AGG + tig0024019:50422-50441 Msa1413270:intron 15.0%
!! TAATATTTGATGTTACTCAA+TGG + tig0024019:48869-48888 Msa1413270:intron 20.0%
!! CTAAATTGTTTATTCAAACA+GGG - tig0024019:49126-49145 None:intergenic 20.0%
!! ACTAAATTGTTTATTCAAAC+AGG - tig0024019:49127-49146 None:intergenic 20.0%
!!! CACTCAAAATCATTTTTTTA+CGG - tig0024019:49165-49184 None:intergenic 20.0%
!!! AGTTAAATGGATAAACTTTT+CGG - tig0024019:50597-50616 None:intergenic 20.0%
!! AATCTATGTTCATAAATGTT+TGG + tig0024019:51386-51405 Msa1413270:three_prime_UTR 20.0%
!! GAAAAATTGTAAGATTATTG+TGG + tig0024019:51410-51429 Msa1413270:three_prime_UTR 20.0%
!! AAGTTATTGAAAAAAAATGC+TGG + tig0024019:51505-51524 Msa1413270:three_prime_UTR 20.0%
!! ATTGGGTATTGTATATATAG+AGG + tig0024019:48278-48297 Msa1413270:five_prime_UTR 25.0%
!! ATATAATTGAAAGATGGGTT+CGG - tig0024019:48367-48386 None:intergenic 25.0%
! TAGTAAAGTCACAGATTAAT+GGG - tig0024019:48793-48812 None:intergenic 25.0%
! TTAGTAAAGTCACAGATTAA+TGG - tig0024019:48794-48813 None:intergenic 25.0%
!! TTTTATTCTCTATGCATTGT+TGG + tig0024019:49042-49061 Msa1413270:intron 25.0%
! TTTATTCTCTATGCATTGTT+GGG + tig0024019:49043-49062 Msa1413270:intron 25.0%
!! TTTTTCCATTGAATGAGTAA+TGG - tig0024019:49093-49112 None:intergenic 25.0%
! ATTCAATGGAAAAACTACTT+AGG + tig0024019:49099-49118 Msa1413270:intron 25.0%
! TTCAATGGAAAAACTACTTA+GGG + tig0024019:49100-49119 Msa1413270:intron 25.0%
! TATTTGATATCTTGATGTGA+AGG + tig0024019:49195-49214 Msa1413270:intron 25.0%
!! CTTCAAGTAGAAACAATTTT+GGG + tig0024019:49280-49299 Msa1413270:intron 25.0%
!! TTCAAGTAGAAACAATTTTG+GGG + tig0024019:49281-49300 Msa1413270:intron 25.0%
! CAAAATCAATTATACTCTAG+AGG + tig0024019:49305-49324 Msa1413270:intron 25.0%
! CATAAGTGCTTATACTAAAA+GGG - tig0024019:49423-49442 None:intergenic 25.0%
! TCATAAGTGCTTATACTAAA+AGG - tig0024019:49424-49443 None:intergenic 25.0%
!!! ATCTTTAAGCTTTTGGTTTT+TGG + tig0024019:49732-49751 Msa1413270:intron 25.0%
! AAATAATACAGACAGGAAAA+TGG - tig0024019:49922-49941 None:intergenic 25.0%
! TTATTTATTGCAGAAGAGTT+AGG + tig0024019:49936-49955 Msa1413270:intron 25.0%
!! TTTAGAAGCTAATTCAAGTT+GGG + tig0024019:50294-50313 Msa1413270:intron 25.0%
! TCCTTACGATTTATAAGTTA+AGG - tig0024019:50320-50339 None:intergenic 25.0%
! GTTGTTAGTTAAAATACTCA+TGG + tig0024019:50525-50544 Msa1413270:intron 25.0%
! TTATACAATGACTAACATTG+TGG - tig0024019:50631-50650 None:intergenic 25.0%
! TAATAGATTCACAATAAGTC+CGG + tig0024019:50648-50667 Msa1413270:intron 25.0%
! TAACTATGATCAAATCAGTA+TGG - tig0024019:51332-51351 None:intergenic 25.0%
! AAGACTTTATGCTTATGTAA+TGG - tig0024019:51531-51550 None:intergenic 25.0%
!!! ACAATATTTTTTTTTGTCGG+AGG - tig0024019:51659-51678 None:intergenic 25.0%
TATGGCGAAAAAGAGAAAAT+CGG + tig0024019:48429-48448 Msa1413270:exon 30.0%
TAAAAGAAGAAGAAGAAGGA+AGG - tig0024019:48600-48619 None:intergenic 30.0%
TAAAATGCAAGAACGAAATC+TGG - tig0024019:48756-48775 None:intergenic 30.0%
! AGATTTCGTTCTTGCATTTT+AGG + tig0024019:48755-48774 Msa1413270:intron 30.0%
! GATTTCGTTCTTGCATTTTA+GGG + tig0024019:48756-48775 Msa1413270:intron 30.0%
TTATAAACAAAATTAGGGGC+TGG - tig0024019:48852-48871 None:intergenic 30.0%
ATTCTTAGACAACAACAAGA+AGG + tig0024019:48973-48992 Msa1413270:CDS 30.0%
! GCTTCAAGTAGAAACAATTT+TGG + tig0024019:49279-49298 Msa1413270:intron 30.0%
! TCAAGTAGAAACAATTTTGG+GGG + tig0024019:49282-49301 Msa1413270:intron 30.0%
AGAGTTGAATTTGACATGTT+TGG - tig0024019:49333-49352 None:intergenic 30.0%
GCTACAAATAAGTGTATGTT+TGG - tig0024019:49395-49414 None:intergenic 30.0%
AAACTATCCTGAAAAACTCA+TGG + tig0024019:49514-49533 Msa1413270:intron 30.0%
! CTTATTTCCATGAGTTTTTC+AGG - tig0024019:49524-49543 None:intergenic 30.0%
TAAGCATTTGTGACATTGTT+CGG - tig0024019:49594-49613 None:intergenic 30.0%
TATCATTGCCTATAGCTTAA+AGG + tig0024019:49661-49680 Msa1413270:intron 30.0%
! AGTGTGAAACTGTTATTTAG+TGG + tig0024019:49704-49723 Msa1413270:intron 30.0%
! GGACATTATCTTTAAGCTTT+TGG + tig0024019:49725-49744 Msa1413270:intron 30.0%
! GGTTTATCAACCTTTTACTT+GGG - tig0024019:49816-49835 None:intergenic 30.0%
!!! TTTTGGTGTTAGATTAGTCA+TGG + tig0024019:49875-49894 Msa1413270:intron 30.0%
CTGCAATAAATAATACAGAC+AGG - tig0024019:49929-49948 None:intergenic 30.0%
! CTTTAGAAGCTAATTCAAGT+TGG + tig0024019:50293-50312 Msa1413270:intron 30.0%
GCCTTAACTTATAAATCGTA+AGG + tig0024019:50316-50335 Msa1413270:intron 30.0%
!! GAAGTCCTTATGAGTTTTAT+AGG + tig0024019:50338-50357 Msa1413270:intron 30.0%
ACACACCTATAAAACTCATA+AGG - tig0024019:50346-50365 None:intergenic 30.0%
ACACATACTGATAATAAGCA+AGG - tig0024019:50571-50590 None:intergenic 30.0%
! CCTTTTATTGAATTTTGCCT+GGG + tig0024019:50671-50690 Msa1413270:intron 30.0%
AGCATTAGAGCAAAATCTTA+TGG - tig0024019:50713-50732 None:intergenic 30.0%
TATAGTTCAACCATCAAGAT+CGG - tig0024019:50746-50765 None:intergenic 30.0%
ACATAATATGTGGGAAACTA+CGG - tig0024019:50812-50831 None:intergenic 30.0%
AACTGTAGGACATAATATGT+GGG - tig0024019:50821-50840 None:intergenic 30.0%
TAACTGTAGGACATAATATG+TGG - tig0024019:50822-50841 None:intergenic 30.0%
TGTGCAATAATGTTAACTGT+AGG - tig0024019:50835-50854 None:intergenic 30.0%
ATGTAGAATGAGCTTGAATA+TGG + tig0024019:50933-50952 Msa1413270:intron 30.0%
!! GTTAGTTTGAATTGCTGTTT+GGG - tig0024019:51006-51025 None:intergenic 30.0%
! AGTTAGTTTGAATTGCTGTT+TGG - tig0024019:51007-51026 None:intergenic 30.0%
TACTGAGTTCTTTACTTGAT+CGG - tig0024019:51099-51118 None:intergenic 30.0%
ATACACCATTTCATTGTTAC+GGG - tig0024019:51225-51244 None:intergenic 30.0%
GATACACCATTTCATTGTTA+CGG - tig0024019:51226-51245 None:intergenic 30.0%
TGATTTGATCATAGTTAGAG+TGG + tig0024019:51335-51354 Msa1413270:exon 30.0%
GATTTGATCATAGTTAGAGT+GGG + tig0024019:51336-51355 Msa1413270:exon 30.0%
AACAAAGTATACTCTCATGT+TGG - tig0024019:51483-51502 None:intergenic 30.0%
TAAGCATAAAGTCTTACTAG+TGG + tig0024019:51536-51555 Msa1413270:three_prime_UTR 30.0%
AAGCATAAAGTCTTACTAGT+GGG + tig0024019:51537-51556 Msa1413270:three_prime_UTR 30.0%
TATTTGCTTCAGATTTCTCA+AGG + tig0024019:51563-51582 Msa1413270:three_prime_UTR 30.0%
! ATAAGAGTGTGTTAATGTGA+GGG - tig0024019:51716-51735 None:intergenic 30.0%
! AATAAGAGTGTGTTAATGTG+AGG - tig0024019:51717-51736 None:intergenic 30.0%
! GGGTATTGTATATATAGAGG+AGG + tig0024019:48281-48300 Msa1413270:five_prime_UTR 35.0%
!!! TTTTTGTTGATTCATGGCGT+GGG - tig0024019:48536-48555 None:intergenic 35.0%
!!! GGAGAGTTTTTGTTGATTCA+TGG - tig0024019:48542-48561 None:intergenic 35.0%
!! TGGTGTTTTTAATCCATTGC+AGG + tig0024019:48933-48952 Msa1413270:intron 35.0%
! TGATACATTTTCTCCTGCAA+TGG - tig0024019:48949-48968 None:intergenic 35.0%
! ATGCATTGTTGGGTTCTTTT+TGG + tig0024019:49053-49072 Msa1413270:intron 35.0%
! TGCATTGTTGGGTTCTTTTT+GGG + tig0024019:49054-49073 Msa1413270:intron 35.0%
CTGAACCATTACTCATTCAA+TGG + tig0024019:49085-49104 Msa1413270:intron 35.0%
! GGAGGCAAAATTGATTCAAT+AGG - tig0024019:49359-49378 None:intergenic 35.0%
!! TGTATGTTTGGTTCCACTTT+TGG - tig0024019:49383-49402 None:intergenic 35.0%
!! ACATGCTCATGAGCTATTTT+CGG - tig0024019:49547-49566 None:intergenic 35.0%
AAGCATTTGTGACATTGTTC+GGG - tig0024019:49593-49612 None:intergenic 35.0%
! ACTCGTTTGAGTTTATCTAC+TGG - tig0024019:49619-49638 None:intergenic 35.0%
CAAAGTGACCTTTAAGCTAT+AGG - tig0024019:49672-49691 None:intergenic 35.0%
AGTTTCACACTCAAAGCAAT+CGG - tig0024019:49695-49714 None:intergenic 35.0%
!!! TTTTGGTTTTTGGTAAGCCA+AGG + tig0024019:49742-49761 Msa1413270:intron 35.0%
! GGGTTTATCAACCTTTTACT+TGG - tig0024019:49817-49836 None:intergenic 35.0%
GGCACAAGTGATAGATATTT+GGG - tig0024019:49837-49856 None:intergenic 35.0%
TGGCACAAGTGATAGATATT+TGG - tig0024019:49838-49857 None:intergenic 35.0%
! TGGTCTAGCTTCTAACTAAT+AGG + tig0024019:50014-50033 Msa1413270:intron 35.0%
! GTTCTAACGTAAGTTGAAAC+TGG + tig0024019:50131-50150 Msa1413270:intron 35.0%
!! CTTCTAAAGCTCAAACACAT+GGG - tig0024019:50282-50301 None:intergenic 35.0%
GGTTGCAATCAAGAGTTAAA+TGG - tig0024019:50610-50629 None:intergenic 35.0%
GGCAAAATTCAATAAAAGGC+CGG - tig0024019:50670-50689 None:intergenic 35.0%
! GCCTTTTATTGAATTTTGCC+TGG + tig0024019:50670-50689 Msa1413270:intron 35.0%
CCCAGGCAAAATTCAATAAA+AGG - tig0024019:50674-50693 None:intergenic 35.0%
! TTTATTGAATTTTGCCTGGG+TGG + tig0024019:50674-50693 Msa1413270:intron 35.0%
! TTATTGAATTTTGCCTGGGT+GGG + tig0024019:50675-50694 Msa1413270:intron 35.0%
TATATCTACATCCGGATATC+AGG - tig0024019:50893-50912 None:intergenic 35.0%
CCAAAGGTTATATCTACATC+CGG - tig0024019:50901-50920 None:intergenic 35.0%
AACAGTACTTCCAAATGCAT+TGG - tig0024019:51048-51067 None:intergenic 35.0%
TTCGAGTAATGTCATGTCAT+CGG + tig0024019:51190-51209 Msa1413270:CDS 35.0%
AATGAAATGGTGTATCCTAG+TGG + tig0024019:51230-51249 Msa1413270:CDS 35.0%
ACGAATTTGAATCTCTGTTC+TGG - tig0024019:51256-51275 None:intergenic 35.0%
AGCTTCAAACTGGAAAACAA+TGG - tig0024019:51459-51478 None:intergenic 35.0%
AAGAGAAGACTGGAACAATG+GGG - tig0024019:48314-48333 None:intergenic 40.0%
GAAGAGAAGACTGGAACAAT+GGG - tig0024019:48315-48334 None:intergenic 40.0%
AGAAGAGAAGACTGGAACAA+TGG - tig0024019:48316-48335 None:intergenic 40.0%
GAAGTAAGAGAAGAGAAGAC+TGG - tig0024019:48324-48343 None:intergenic 40.0%
!!! GTTTTTGTTGATTCATGGCG+TGG - tig0024019:48537-48556 None:intergenic 40.0%
CAAAAACTCTCCTTTCAAGC+CGG + tig0024019:48550-48569 Msa1413270:CDS 40.0%
GGGGTAAAAGAAGAAGAAGA+AGG - tig0024019:48604-48623 None:intergenic 40.0%
TGCAGGAGAAAATGTATCAG+TGG + tig0024019:48950-48969 Msa1413270:intron 40.0%
TGATATAGTCTCTCACTTGC+AGG + tig0024019:49014-49033 Msa1413270:CDS 40.0%
! AATTTTGCCTCCTCCAAAAG+TGG + tig0024019:49367-49386 Msa1413270:intron 40.0%
!! ATGTTTGGTTCCACTTTTGG+AGG - tig0024019:49380-49399 None:intergenic 40.0%
TATAGGCAATGATAGACCTG+GGG - tig0024019:49655-49674 None:intergenic 40.0%
CTATAGGCAATGATAGACCT+GGG - tig0024019:49656-49675 None:intergenic 40.0%
GGATCAAACTCCCAAGTAAA+AGG + tig0024019:49803-49822 Msa1413270:intron 40.0%
! CACCAAAAGTATAACCGACA+CGG - tig0024019:49863-49882 None:intergenic 40.0%
AGAGTTAGGTCCTATGTGAA+AGG + tig0024019:49950-49969 Msa1413270:intron 40.0%
GAGTTAGGTCCTATGTGAAA+GGG + tig0024019:49951-49970 Msa1413270:intron 40.0%
AGTTAGAAGCTAGACCAAAC+TGG - tig0024019:50011-50030 None:intergenic 40.0%
!! GCTTCTAAAGCTCAAACACA+TGG - tig0024019:50283-50302 None:intergenic 40.0%
ATGTATCAGTCCGATCTTGA+TGG + tig0024019:50733-50752 Msa1413270:intron 40.0%
! ACAACTTTTCTCGCTTCACT+TGG + tig0024019:50785-50804 Msa1413270:intron 40.0%
TTCGAATGTCTCCTGATATC+CGG + tig0024019:50879-50898 Msa1413270:intron 40.0%
CCGGATGTAGATATAACCTT+TGG + tig0024019:50898-50917 Msa1413270:intron 40.0%
ACATGCAAACACATAGCCAA+AGG - tig0024019:50917-50936 None:intergenic 40.0%
TTCTGTTGAATTGACTGCCT+TGG - tig0024019:50981-51000 None:intergenic 40.0%
GTACTTCCAAATGCATTGGA+CGG - tig0024019:51044-51063 None:intergenic 40.0%
GTGCTAAATGATATGGCTGA+AGG - tig0024019:51157-51176 None:intergenic 40.0%
TCAGCCATATCATTTAGCAC+AGG + tig0024019:51157-51176 Msa1413270:CDS 40.0%
CAGCCATATCATTTAGCACA+GGG + tig0024019:51158-51177 Msa1413270:CDS 40.0%
GATGACATGACATTACTCGA+AGG - tig0024019:51191-51210 None:intergenic 40.0%
TCGAGTAATGTCATGTCATC+GGG + tig0024019:51191-51210 Msa1413270:CDS 40.0%
CAGTATGGAAAATCATGACC+AGG - tig0024019:51317-51336 None:intergenic 40.0%
AGTGGGTAACAGTAAAGAGA+AGG + tig0024019:51353-51372 Msa1413270:three_prime_UTR 40.0%
TCATGTTGGAAGCTTCAAAC+TGG - tig0024019:51469-51488 None:intergenic 40.0%
CTTCAGATTTCTCAAGGCTT+AGG + tig0024019:51569-51588 Msa1413270:three_prime_UTR 40.0%
AGAGAAGACTGGAACAATGG+GGG - tig0024019:48313-48332 None:intergenic 45.0%
TCTTCTCTTACTTCTCTGCC+CGG + tig0024019:48330-48349 Msa1413270:five_prime_UTR 45.0%
!! ATTGAAAGATGGGTTCGGTG+CGG - tig0024019:48362-48381 None:intergenic 45.0%
ACCAATCCTCCTTCCTTGAT+CGG + tig0024019:48400-48419 Msa1413270:five_prime_UTR 45.0%
ATATCTCCACCGATCAAGGA+AGG - tig0024019:48412-48431 None:intergenic 45.0%
TTCCTTGATCGGTGGAGATA+TGG + tig0024019:48411-48430 Msa1413270:exon 45.0%
GGCGAAAAAGAGAAAATCGG+TGG + tig0024019:48432-48451 Msa1413270:CDS 45.0%
GAGATGAGAGAGGGAATTAG+CGG - tig0024019:48509-48528 None:intergenic 45.0%
GTGGGTATAGAGATGAGAGA+GGG - tig0024019:48518-48537 None:intergenic 45.0%
CAAAATTAGGGGCTGGAATG+TGG - tig0024019:48845-48864 None:intergenic 45.0%
!! TTTGGTTCCACTTTTGGAGG+AGG - tig0024019:49377-49396 None:intergenic 45.0%
TCAAACGAGTCAGTCCAAAC+AGG + tig0024019:49629-49648 Msa1413270:intron 45.0%
GCTATAGGCAATGATAGACC+TGG - tig0024019:49657-49676 None:intergenic 45.0%
AAAGTATAACCGACACGGTG+TGG - tig0024019:49858-49877 None:intergenic 45.0%
!!! CACCGTGTCGGTTATACTTT+TGG + tig0024019:49858-49877 Msa1413270:intron 45.0%
TTAGGTCCTATGTGAAAGGG+AGG + tig0024019:49954-49973 Msa1413270:intron 45.0%
TAGGTCCTATGTGAAAGGGA+GGG + tig0024019:49955-49974 Msa1413270:intron 45.0%
AGTGCAGCTTAAGTCCAGTT+TGG + tig0024019:49994-50013 Msa1413270:intron 45.0%
! TTGCCTGGGTGGGATTTTTA+TGG + tig0024019:50685-50704 Msa1413270:intron 45.0%
TGAGCTTGAATATGGAGCAG+AGG + tig0024019:50941-50960 Msa1413270:CDS 45.0%
AGGAATCACCAGTTTCTCCA+AGG + tig0024019:50961-50980 Msa1413270:CDS 45.0%
ATTGACTGCCTTGGAGAAAC+TGG - tig0024019:50972-50991 None:intergenic 45.0%
TCCATACCGTCCAATGCATT+TGG + tig0024019:51035-51054 Msa1413270:CDS 45.0%
TCCAAATGCATTGGACGGTA+TGG - tig0024019:51039-51058 None:intergenic 45.0%
!! GCATTTGGAAGTACTGTTGC+AGG + tig0024019:51050-51069 Msa1413270:CDS 45.0%
CTGCCCTGTGCTAAATGATA+TGG - tig0024019:51164-51183 None:intergenic 45.0%
CGAGTAATGTCATGTCATCG+GGG + tig0024019:51192-51211 Msa1413270:CDS 45.0%
AATGTCATGTCATCGGGGAA+CGG + tig0024019:51197-51216 Msa1413270:CDS 45.0%
CGGAACCCGTAACAATGAAA+TGG + tig0024019:51217-51236 Msa1413270:CDS 45.0%
ATGCATGTCCATGCAATCAC+TGG - tig0024019:51285-51304 None:intergenic 45.0%
!! AAATATTATAAACAAAATTA+GGG - tig0024019:48857-48876 None:intergenic 5.0%
ACCGATCAAGGAAGGAGGAT+TGG - tig0024019:48404-48423 None:intergenic 50.0%
AATCCTCCTTCCTTGATCGG+TGG + tig0024019:48403-48422 Msa1413270:five_prime_UTR 50.0%
ATGAGAGAGGGAATTAGCGG+TGG - tig0024019:48506-48525 None:intergenic 50.0%
CGTGGGTATAGAGATGAGAG+AGG - tig0024019:48519-48538 None:intergenic 50.0%
! GAAGGAAGCATACAAGAGCG+TGG - tig0024019:48582-48601 None:intergenic 50.0%
ACTTGGGAGTTTGATCCCGA+GGG - tig0024019:49800-49819 None:intergenic 50.0%
TACTTGGGAGTTTGATCCCG+AGG - tig0024019:49801-49820 None:intergenic 50.0%
AGGTCCTATGTGAAAGGGAG+GGG + tig0024019:49956-49975 Msa1413270:intron 50.0%
GTGCCATAAAAATCCCACCC+AGG - tig0024019:50691-50710 None:intergenic 50.0%
TGCATTGGACGGTATGGATG+CGG - tig0024019:51033-51052 None:intergenic 50.0%
!! TGGAAGTACTGTTGCAGGAC+AGG + tig0024019:51055-51074 Msa1413270:CDS 50.0%
!! GGAAGTACTGTTGCAGGACA+GGG + tig0024019:51056-51075 Msa1413270:CDS 50.0%
!! GAAGTACTGTTGCAGGACAG+GGG + tig0024019:51057-51076 Msa1413270:CDS 50.0%
GAATCTCTGTTCTGGCCACT+AGG - tig0024019:51248-51267 None:intergenic 50.0%
GTCCAACTCCAGTGATTGCA+TGG + tig0024019:51274-51293 Msa1413270:CDS 50.0%
GTCCATGCAATCACTGGAGT+TGG - tig0024019:51279-51298 None:intergenic 50.0%
TCTCCACCGATCAAGGAAGG+AGG - tig0024019:48409-48428 None:intergenic 55.0%
CGCCATATCTCCACCGATCA+AGG - tig0024019:48416-48435 None:intergenic 55.0%
GGAGTCTCGTCTCGATGAAG+TGG + tig0024019:48453-48472 Msa1413270:CDS 55.0%
GGTGCAGAAGGTGGAGTACA+TGG - tig0024019:48485-48504 None:intergenic 55.0%
ATTAGCGGTGGTGCAGAAGG+TGG - tig0024019:48494-48513 None:intergenic 55.0%
GGAATTAGCGGTGGTGCAGA+AGG - tig0024019:48497-48516 None:intergenic 55.0%
! TACAAGAGCGTGGCGTTCAC+CGG - tig0024019:48572-48591 None:intergenic 55.0%
TGATAGACCTGGGGCCTGTT+TGG - tig0024019:49646-49665 None:intergenic 55.0%
! CAAGGTATCATCCCCTCGCA+CGG + tig0024019:49760-49779 Msa1413270:intron 55.0%
GGCTGGGGAGATTAATCCCT+CGG + tig0024019:49781-49800 Msa1413270:intron 55.0%
GCTGGGGAGATTAATCCCTC+GGG + tig0024019:49782-49801 Msa1413270:intron 55.0%
TCACTTGTGCCACACCGTGT+CGG + tig0024019:49846-49865 Msa1413270:intron 55.0%
GGTCCTATGTGAAAGGGAGG+GGG + tig0024019:49957-49976 Msa1413270:intron 55.0%
AAGCCCCCTCCCTTTCACAT+AGG - tig0024019:49963-49982 None:intergenic 55.0%
TATGTGAAAGGGAGGGGGCT+TGG + tig0024019:49962-49981 Msa1413270:intron 55.0%
CAGGACAGGGGATACGAACT+GGG + tig0024019:51069-51088 Msa1413270:CDS 55.0%
GACATGCATGCAGACAGTCC+TGG + tig0024019:51296-51315 Msa1413270:CDS 55.0%
!! GTGGCGTTCACCGGCTTGAA+AGG - tig0024019:48563-48582 None:intergenic 60.0%
AGTCAGTCCAAACAGGCCCC+AGG + tig0024019:49636-49655 Msa1413270:intron 60.0%
CGTGCGAGGGGATGATACCT+TGG - tig0024019:49762-49781 None:intergenic 60.0%
TATCATCCCCTCGCACGGCT+GGG + tig0024019:49765-49784 Msa1413270:intron 60.0%
TAATCTCCCCAGCCGTGCGA+GGG - tig0024019:49775-49794 None:intergenic 60.0%
TTAATCTCCCCAGCCGTGCG+AGG - tig0024019:49776-49795 None:intergenic 60.0%
GCAGGACAGGGGATACGAAC+TGG + tig0024019:51068-51087 Msa1413270:CDS 60.0%
GCTGAAGGCTGCGACGAATG+GGG - tig0024019:51142-51161 None:intergenic 60.0%
GGCTGAAGGCTGCGACGAAT+GGG - tig0024019:51143-51162 None:intergenic 60.0%
TGGCTGAAGGCTGCGACGAA+TGG - tig0024019:51144-51163 None:intergenic 60.0%
GTATCATCCCCTCGCACGGC+TGG + tig0024019:49764-49783 Msa1413270:intron 65.0%
ATCATCCCCTCGCACGGCTG+GGG + tig0024019:49766-49785 Msa1413270:intron 65.0%
AATCTCCCCAGCCGTGCGAG+GGG - tig0024019:49774-49793 None:intergenic 65.0%
!! GGTTCGGTGCGGTGCTTGCC+GGG - tig0024019:48351-48370 None:intergenic 70.0%
!! GGGTTCGGTGCGGTGCTTGC+CGG - tig0024019:48352-48371 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0024019 gene 48278 51739 48278 ID=Msa1413270;Name=Msa1413270
tig0024019 mRNA 48278 51739 48278 ID=Msa1413270-mRNA-1;Parent=Msa1413270;Name=Msa1413270-mRNA-1;_AED=0.00;_eAED=0.00;_QI=152|1|1|1|1|1|3|402|212
tig0024019 exon 48278 48588 48278 ID=Msa1413270-mRNA-1:exon:21017;Parent=Msa1413270-mRNA-1
tig0024019 exon 48955 49035 48955 ID=Msa1413270-mRNA-1:exon:21018;Parent=Msa1413270-mRNA-1
tig0024019 exon 50939 51739 50939 ID=Msa1413270-mRNA-1:exon:21019;Parent=Msa1413270-mRNA-1
tig0024019 five_prime_UTR 48278 48429 48278 ID=Msa1413270-mRNA-1:five_prime_utr;Parent=Msa1413270-mRNA-1
tig0024019 CDS 48430 48588 48430 ID=Msa1413270-mRNA-1:cds;Parent=Msa1413270-mRNA-1
tig0024019 CDS 48955 49035 48955 ID=Msa1413270-mRNA-1:cds;Parent=Msa1413270-mRNA-1
tig0024019 CDS 50939 51337 50939 ID=Msa1413270-mRNA-1:cds;Parent=Msa1413270-mRNA-1
tig0024019 three_prime_UTR 51338 51739 51338 ID=Msa1413270-mRNA-1:three_prime_utr;Parent=Msa1413270-mRNA-1
Gene Sequence

>Msa1413270

ATGGCGAAAAAGAGAAAATCGGTGGAGTCTCGTCTCGATGAAGTGGATCGCACCATGTACTCCACCTTCTGCACCACCGCTAATTCCCTCTCTCATCTCTATACCCACGCCATGAATCAACAAAAACTCTCCTTTCAAGCCGGTGAACGCCACGCTCTTGAGAAAATGTATCAGTGGATTCTTAGACAACAACAAGAAGGAATGAGAGTCACTACTATTGATATAGTCTCTCACTTGCAGAATGAGCTTGAATATGGAGCAGAGGAATCACCAGTTTCTCCAAGGCAGTCAATTCAACAGAACTCCCAAACAGCAATTCAAACTAACTTTGCCGCATCCATACCGTCCAATGCATTTGGAAGTACTGTTGCAGGACAGGGGATACGAACTGGGCAAACCGATCAAGTAAAGAACTCAGTATTCTCAAATGCACTCTCTAGCCCCATTCGTCGCAGCCTTCAGCCATATCATTTAGCACAGGGCAGCAATCCTTCGAGTAATGTCATGTCATCGGGGAACGGAACCCGTAACAATGAAATGGTGTATCCTAGTGGCCAGAACAGAGATTCAAATTCGTCCAACTCCAGTGATTGCATGGACATGCATGCAGACAGTCCTGGTCATGATTTTCCATACTGA
Protein sequence

>Msa1413270

MAKKRKSVESRLDEVDRTMYSTFCTTANSLSHLYTHAMNQQKLSFQAGERHALEKMYQWILRQQQEGMRVTTIDIVSHLQNELEYGAEESPVSPRQSIQQNSQTAIQTNFAASIPSNAFGSTVAGQGIRTGQTDQVKNSVFSNALSSPIRRSLQPYHLAQGSNPSSNVMSSGNGTRNNEMVYPSGQNRDSNSSNSSDCMDMHADSPGHDFPY*