Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1364470 | XP_003630138.1 | 99.528 | 212 | 1 | 0 | 1 | 212 | 1 | 212 | 4.46e-157 | 443 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000550 | Msa1364470 | 0.862646 | 4.372770e-64 | -8.615850e-47 |
| Msa0001590 | Msa1364470 | -0.813938 | 1.986636e-51 | -8.615850e-47 |
| Msa0014920 | Msa1364470 | -0.808775 | 2.623992e-50 | -8.615850e-47 |
| Msa0016670 | Msa1364470 | -0.836796 | 7.585601e-57 | -8.615850e-47 |
| Msa0017510 | Msa1364470 | -0.845481 | 3.953722e-59 | -8.615850e-47 |
| Msa0018250 | Msa1364470 | -0.811641 | 6.325772e-51 | -8.615850e-47 |
| Msa0028060 | Msa1364470 | -0.824337 | 8.524203e-54 | -8.615850e-47 |
| Msa0029540 | Msa1364470 | 0.823341 | 1.459310e-53 | -8.615850e-47 |
| Msa0032370 | Msa1364470 | -0.838936 | 2.138453e-57 | -8.615850e-47 |
| Msa0032840 | Msa1364470 | 0.855890 | 4.654740e-62 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1364470 | MtrunA17_Chr8g0383401 | 99.528 | 212 | 1 | 0 | 1 | 212 | 1 | 212 | 4.11e-161 | 443 |
| Msa1364470 | MtrunA17_Chr6g0467101 | 50.711 | 211 | 81 | 5 | 1 | 204 | 1 | 195 | 2.32e-62 | 192 |
| Msa1364470 | MtrunA17_Chr1g0201541 | 49.519 | 208 | 100 | 5 | 1 | 204 | 1 | 207 | 1.42e-58 | 183 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1364470 | AT4G09830.1 | 51.402 | 214 | 71 | 6 | 1 | 204 | 1 | 191 | 1.37e-55 | 175 |
| Msa1364470 | AT5G64780.1 | 46.591 | 176 | 86 | 4 | 1 | 174 | 1 | 170 | 1.55e-43 | 144 |
Find 92 sgRNAs with CRISPR-Local
Find 244 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAAAGTATACTCTCATGT+TGG | 0.542600 | tig0018152:+15700 | None:intergenic |
| AACAGTACTTCCAAATGCAT+TGG | 0.577353 | tig0018152:+16135 | None:intergenic |
| AAGACTTTATGCTTATGTAA+TGG | 0.479258 | tig0018152:+15652 | None:intergenic |
| AAGAGAAGACTGGAACAATG+GGG | 0.698423 | tig0018152:+18876 | None:intergenic |
| AAGCATAAAGTCTTACTAGT+GGG | 0.618970 | tig0018152:-15643 | Msa1364470:three_prime_UTR |
| AATAAGAGTGTGTTAATGTG+AGG | 0.579886 | tig0018152:+15466 | None:intergenic |
| AATCCTCCTTCCTTGATCGG+TGG | 0.614077 | tig0018152:-18784 | Msa1364470:five_prime_UTR |
| AATCTATGTTCATAAATGTT+TGG | 0.466154 | tig0018152:-15794 | Msa1364470:three_prime_UTR |
| AATGAAATGGTGTATCCTAG+TGG | 0.663062 | tig0018152:-15950 | Msa1364470:CDS |
| AATGTCATGTCATCGGGGAA+CGG | 0.595236 | tig0018152:-15983 | Msa1364470:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATGGCGAAAAAGAGAAAAT+CGG | - | tig0018152:15614-15633 | Msa1364470:three_prime_UTR | 30.0% | |
| TAAAAGAAGAAGAAGAAGGA+AGG | + | tig0018152:15785-15804 | None:intergenic | 30.0% | |
| TAAAATGCAAGAACGAAATC+TGG | + | tig0018152:15941-15960 | None:intergenic | 30.0% | |
| TTATAAACAAAATTAGGGGC+TGG | + | tig0018152:16037-16056 | None:intergenic | 30.0% | |
| ATTCTTAGACAACAACAAGA+AGG | - | tig0018152:16158-16177 | Msa1364470:CDS | 30.0% | |
| AGAGTTGAATTTGACATGTT+TGG | + | tig0018152:16518-16537 | None:intergenic | 30.0% | |
| GCTACAAATAAGTGTATGTT+TGG | + | tig0018152:16580-16599 | None:intergenic | 30.0% | |
| AAACTATCCTGAAAAACTCA+TGG | - | tig0018152:16699-16718 | Msa1364470:intron | 30.0% | |
| TAAGCATTTGTGACATTGTT+CGG | + | tig0018152:16779-16798 | None:intergenic | 30.0% | |
| TATCATTGCCTATAGCTTAA+AGG | - | tig0018152:16846-16865 | Msa1364470:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0018152 | gene | 15463 | 18931 | 15463 | ID=Msa1364470;Name=Msa1364470 |
| tig0018152 | mRNA | 15463 | 18931 | 15463 | ID=Msa1364470-mRNA-1;Parent=Msa1364470;Name=Msa1364470-mRNA-1;_AED=0.00;_eAED=0.00;_QI=152|1|1|1|1|1|3|402|212 |
| tig0018152 | exon | 15463 | 16263 | 15463 | ID=Msa1364470-mRNA-1:exon:14055;Parent=Msa1364470-mRNA-1 |
| tig0018152 | exon | 18174 | 18254 | 18174 | ID=Msa1364470-mRNA-1:exon:14054;Parent=Msa1364470-mRNA-1 |
| tig0018152 | exon | 18621 | 18931 | 18621 | ID=Msa1364470-mRNA-1:exon:14053;Parent=Msa1364470-mRNA-1 |
| tig0018152 | five_prime_UTR | 18780 | 18931 | 18780 | ID=Msa1364470-mRNA-1:five_prime_utr;Parent=Msa1364470-mRNA-1 |
| tig0018152 | CDS | 18621 | 18779 | 18621 | ID=Msa1364470-mRNA-1:cds;Parent=Msa1364470-mRNA-1 |
| tig0018152 | CDS | 18174 | 18254 | 18174 | ID=Msa1364470-mRNA-1:cds;Parent=Msa1364470-mRNA-1 |
| tig0018152 | CDS | 15865 | 16263 | 15865 | ID=Msa1364470-mRNA-1:cds;Parent=Msa1364470-mRNA-1 |
| tig0018152 | three_prime_UTR | 15463 | 15864 | 15463 | ID=Msa1364470-mRNA-1:three_prime_utr;Parent=Msa1364470-mRNA-1 |
| Gene Sequence |
>Msa1364470 ATGGCGAAAAAGAGAAAATCGGTGGAGTCTCGTCTCGATGAAGTGGATCGCACCATGTACTCCACCTTCTGCACCACCGCTAATTCCCTCTCTCATCTCTATACCCACGCCATGAATCAACAAAAACTCTCCTTTCAAGCCGGTGAACGCCACGCTCTTGAGAAAATGTATCAGTGGATTCTTAGACAACAACAAGAAGGAATGAGAGTCACTACTATTGATATAGTCTCTCACTTGCAGAATGAGCTTGAATATGGAGCAGAGGAATCACCAGTTTCTCCAAGGCAGTCAATTCAACAGAACTCCCAAACAGCAATTCAAACTAACTTTGCCGCATCCATACCGTCCAATGCATTTGGAAGTACTGTTGCAGGACAGGGGATACGAACTGGGCAAACCGATCAAGTAAAGAACTCAGTATTCTCAAATGCACTCTCTAGCCCCATTCGTCGCAGCCTTCAGCCATATCATTTAGCACAGGGCAGCAATCCTTCGAGTAATGTCATGTCATCGGGGAACGGAACCCGTAACAATGAAATGGTGTATCCTAGTGGCCAGAACAGAGATTCAAATTCGTCCAACTCCAGTGATTGCATGGACATGCATGCAGACAGTCCTGGTCATGATTTTCCATACTGA |
| Protein sequence |
>Msa1364470 MAKKRKSVESRLDEVDRTMYSTFCTTANSLSHLYTHAMNQQKLSFQAGERHALEKMYQWILRQQQEGMRVTTIDIVSHLQNELEYGAEESPVSPRQSIQQNSQTAIQTNFAASIPSNAFGSTVAGQGIRTGQTDQVKNSVFSNALSSPIRRSLQPYHLAQGSNPSSNVMSSGNGTRNNEMVYPSGQNRDSNSSNSSDCMDMHADSPGHDFPY* |
