AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1425680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1425680 MtrunA17_Chr8g0383401 99.528 212 1 0 1 212 1 212 4.11e-161 443
Msa1425680 MtrunA17_Chr6g0467101 50.711 211 81 5 1 204 1 195 2.32e-62 192
Msa1425680 MtrunA17_Chr1g0201541 49.519 208 100 5 1 204 1 207 1.42e-58 183
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1425680 AT4G09830.1 51.402 214 71 6 1 204 1 191 1.37e-55 175
Msa1425680 AT5G64780.1 46.591 176 86 4 1 174 1 170 1.55e-43 144

Find 92 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTTCGGTGCGGTGCTTGC+CGG 0.312526 tig0025883:-36473 None:intergenic
TGATATAGTCTCTCACTTGC+AGG 0.320186 tig0025883:+37138 Msa1425680:CDS
ACGAATTTGAATCTCTGTTC+TGG 0.321939 tig0025883:-39384 None:intergenic
CTGCCCTGTGCTAAATGATA+TGG 0.346225 tig0025883:-39292 None:intergenic
CTTCAGATTTCTCAAGGCTT+AGG 0.349977 tig0025883:+39700 Msa1425680:three_prime_UTR
GGCTGAAGGCTGCGACGAAT+GGG 0.351898 tig0025883:-39271 None:intergenic
AGTTAGTTTGAATTGCTGTT+TGG 0.364182 tig0025883:-39135 None:intergenic
TAACTATGATCAAATCAGTA+TGG 0.368060 tig0025883:-39460 None:intergenic
GTTAGTTTGAATTGCTGTTT+GGG 0.374257 tig0025883:-39134 None:intergenic
GCATTTGGAAGTACTGTTGC+AGG 0.382751 tig0025883:+39181 Msa1425680:CDS
ATTGACTGCCTTGGAGAAAC+TGG 0.385558 tig0025883:-39100 None:intergenic
CGCCATATCTCCACCGATCA+AGG 0.389326 tig0025883:-36537 None:intergenic
ATACACCATTTCATTGTTAC+GGG 0.393219 tig0025883:-39353 None:intergenic
GGTTCGGTGCGGTGCTTGCC+GGG 0.397100 tig0025883:-36472 None:intergenic
TCCATACCGTCCAATGCATT+TGG 0.397895 tig0025883:+39166 Msa1425680:CDS
GCAGGACAGGGGATACGAAC+TGG 0.401130 tig0025883:+39199 Msa1425680:CDS
TTCCTTGATCGGTGGAGATA+TGG 0.407978 tig0025883:+36535 Msa1425680:exon
TCATGTTGGAAGCTTCAAAC+TGG 0.412934 tig0025883:-39597 None:intergenic
GATACACCATTTCATTGTTA+CGG 0.418071 tig0025883:-39354 None:intergenic
TGGCTGAAGGCTGCGACGAA+TGG 0.418206 tig0025883:-39272 None:intergenic
AATTAATATAATTGAAAGAT+GGG 0.418807 tig0025883:-36493 None:intergenic
TAATTAATATAATTGAAAGA+TGG 0.422990 tig0025883:-36494 None:intergenic
TCGAGTAATGTCATGTCATC+GGG 0.423559 tig0025883:+39322 Msa1425680:CDS
TGGAAGTACTGTTGCAGGAC+AGG 0.432969 tig0025883:+39186 Msa1425680:CDS
ATGTAGAATGAGCTTGAATA+TGG 0.441794 tig0025883:+39064 Msa1425680:intron
GAAGAGAAGACTGGAACAAT+GGG 0.443494 tig0025883:-36436 None:intergenic
ATTGGGTATTGTATATATAG+AGG 0.448947 tig0025883:+36402 Msa1425680:five_prime_UTR
CGGAACCCGTAACAATGAAA+TGG 0.453370 tig0025883:+39348 Msa1425680:CDS
TTCTGTTGAATTGACTGCCT+TGG 0.458544 tig0025883:-39109 None:intergenic
AGAAGAGAAGACTGGAACAA+TGG 0.461600 tig0025883:-36437 None:intergenic
AATCTATGTTCATAAATGTT+TGG 0.466154 tig0025883:+39517 Msa1425680:three_prime_UTR
AAGACTTTATGCTTATGTAA+TGG 0.479258 tig0025883:-39659 None:intergenic
TACTGAGTTCTTTACTTGAT+CGG 0.479541 tig0025883:-39227 None:intergenic
TACAAGAGCGTGGCGTTCAC+CGG 0.488524 tig0025883:-36693 None:intergenic
TCCAAATGCATTGGACGGTA+TGG 0.490213 tig0025883:-39167 None:intergenic
TCTTCTCTTACTTCTCTGCC+CGG 0.492447 tig0025883:+36454 Msa1425680:five_prime_UTR
ACCAATCCTCCTTCCTTGAT+CGG 0.496313 tig0025883:+36524 Msa1425680:five_prime_UTR
GTGGCGTTCACCGGCTTGAA+AGG 0.497863 tig0025883:-36684 None:intergenic
GAGATGAGAGAGGGAATTAG+CGG 0.501181 tig0025883:-36630 None:intergenic
ATATAATTGAAAGATGGGTT+CGG 0.502181 tig0025883:-36488 None:intergenic
GGAAGTACTGTTGCAGGACA+GGG 0.507937 tig0025883:+39187 Msa1425680:CDS
GTCCATGCAATCACTGGAGT+TGG 0.512023 tig0025883:-39407 None:intergenic
ACCGATCAAGGAAGGAGGAT+TGG 0.519506 tig0025883:-36525 None:intergenic
GAAGTAAGAGAAGAGAAGAC+TGG 0.523441 tig0025883:-36445 None:intergenic
GAATCTCTGTTCTGGCCACT+AGG 0.523458 tig0025883:-39376 None:intergenic
TTCGAGTAATGTCATGTCAT+CGG 0.525561 tig0025883:+39321 Msa1425680:CDS
GGAATTAGCGGTGGTGCAGA+AGG 0.533371 tig0025883:-36618 None:intergenic
TGCATTGGACGGTATGGATG+CGG 0.534195 tig0025883:-39161 None:intergenic
AACAAAGTATACTCTCATGT+TGG 0.542600 tig0025883:-39611 None:intergenic
CGTGGGTATAGAGATGAGAG+AGG 0.545674 tig0025883:-36640 None:intergenic
GGAGTCTCGTCTCGATGAAG+TGG 0.547540 tig0025883:+36577 Msa1425680:CDS
TATTTGCTTCAGATTTCTCA+AGG 0.553276 tig0025883:+39694 Msa1425680:three_prime_UTR
GTCCAACTCCAGTGATTGCA+TGG 0.554317 tig0025883:+39405 Msa1425680:CDS
TGATTTGATCATAGTTAGAG+TGG 0.557330 tig0025883:+39466 Msa1425680:exon
CAGCCATATCATTTAGCACA+GGG 0.558095 tig0025883:+39289 Msa1425680:CDS
ATGCATGTCCATGCAATCAC+TGG 0.561386 tig0025883:-39413 None:intergenic
CAGTATGGAAAATCATGACC+AGG 0.574411 tig0025883:-39445 None:intergenic
AACAGTACTTCCAAATGCAT+TGG 0.577353 tig0025883:-39176 None:intergenic
ATTAGCGGTGGTGCAGAAGG+TGG 0.579586 tig0025883:-36615 None:intergenic
AATAAGAGTGTGTTAATGTG+AGG 0.579886 tig0025883:-39845 None:intergenic
TCAGCCATATCATTTAGCAC+AGG 0.580324 tig0025883:+39288 Msa1425680:CDS
GGTGCAGAAGGTGGAGTACA+TGG 0.586968 tig0025883:-36606 None:intergenic
ATAAGAGTGTGTTAATGTGA+GGG 0.588113 tig0025883:-39844 None:intergenic
GACATGCATGCAGACAGTCC+TGG 0.588311 tig0025883:+39427 Msa1425680:CDS
CAGGACAGGGGATACGAACT+GGG 0.588579 tig0025883:+39200 Msa1425680:CDS
AGTGGGTAACAGTAAAGAGA+AGG 0.590826 tig0025883:+39484 Msa1425680:three_prime_UTR
AATGTCATGTCATCGGGGAA+CGG 0.595236 tig0025883:+39328 Msa1425680:CDS
GTGGGTATAGAGATGAGAGA+GGG 0.599340 tig0025883:-36639 None:intergenic
ATTCTTAGACAACAACAAGA+AGG 0.606741 tig0025883:+37097 Msa1425680:CDS
AATCCTCCTTCCTTGATCGG+TGG 0.614077 tig0025883:+36527 Msa1425680:five_prime_UTR
ATATCTCCACCGATCAAGGA+AGG 0.614630 tig0025883:-36533 None:intergenic
TGCAGGAGAAAATGTATCAG+TGG 0.615078 tig0025883:+37074 Msa1425680:intron
GATTTGATCATAGTTAGAGT+GGG 0.618081 tig0025883:+39467 Msa1425680:exon
AAGCATAAAGTCTTACTAGT+GGG 0.618970 tig0025883:+39668 Msa1425680:three_prime_UTR
ATGAGAGAGGGAATTAGCGG+TGG 0.626062 tig0025883:-36627 None:intergenic
TAAGCATAAAGTCTTACTAG+TGG 0.627894 tig0025883:+39667 Msa1425680:three_prime_UTR
TGAGCTTGAATATGGAGCAG+AGG 0.628224 tig0025883:+39072 Msa1425680:CDS
GCTGAAGGCTGCGACGAATG+GGG 0.632700 tig0025883:-39270 None:intergenic
AGGAATCACCAGTTTCTCCA+AGG 0.640475 tig0025883:+39092 Msa1425680:CDS
GAAGTACTGTTGCAGGACAG+GGG 0.643448 tig0025883:+39188 Msa1425680:CDS
AGCTTCAAACTGGAAAACAA+TGG 0.643885 tig0025883:-39587 None:intergenic
GTGCTAAATGATATGGCTGA+AGG 0.646201 tig0025883:-39285 None:intergenic
GGGTATTGTATATATAGAGG+AGG 0.658495 tig0025883:+36405 Msa1425680:five_prime_UTR
AATGAAATGGTGTATCCTAG+TGG 0.663062 tig0025883:+39361 Msa1425680:CDS
GTACTTCCAAATGCATTGGA+CGG 0.665522 tig0025883:-39172 None:intergenic
AGAGAAGACTGGAACAATGG+GGG 0.670119 tig0025883:-36434 None:intergenic
AAGAGAAGACTGGAACAATG+GGG 0.686005 tig0025883:-36435 None:intergenic
GAAGGAAGCATACAAGAGCG+TGG 0.686735 tig0025883:-36703 None:intergenic
TCTCCACCGATCAAGGAAGG+AGG 0.686793 tig0025883:-36530 None:intergenic
GATGACATGACATTACTCGA+AGG 0.723139 tig0025883:-39319 None:intergenic
CGAGTAATGTCATGTCATCG+GGG 0.731834 tig0025883:+39323 Msa1425680:CDS
ATTGAAAGATGGGTTCGGTG+CGG 0.732396 tig0025883:-36483 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATATAATTGAAAGAT+GGG - tig0025883:36496-36515 None:intergenic 10.0%
!! TAATTAATATAATTGAAAGA+TGG - tig0025883:36497-36516 None:intergenic 10.0%
!! AATAATTTGAAAATAAGAAA+GGG - tig0025883:36748-36767 None:intergenic 10.0%
!! TAATAATTTGAAAATAAGAA+AGG - tig0025883:36749-36768 None:intergenic 10.0%
!! AAATAATTAAAAGAAAAGAA+TGG - tig0025883:36810-36829 None:intergenic 10.0%
!! AATATTATAAACAAAATTAG+GGG - tig0025883:36980-36999 None:intergenic 10.0%
!! CAAATATTATAAACAAAATT+AGG - tig0025883:36982-37001 None:intergenic 10.0%
!!! ATTACAATATTTTTTTTTGT+CGG - tig0025883:39793-39812 None:intergenic 10.0%
!! ATAATTTGAAAATAAGAAAG+GGG - tig0025883:36747-36766 None:intergenic 15.0%
!! TGATTTAATATAAAATGCTT+TGG + tig0025883:37037-37056 Msa1425680:intron 15.0%
!!! AATAAGATATTTTATAACAC+AGG + tig0025883:37567-37586 Msa1425680:intron 15.0%
!! GTATATTAAAAGAATAATCT+TGG + tig0025883:38207-38226 Msa1425680:intron 15.0%
!!! TAAGCTTATATAATTTTATG+AGG - tig0025883:38323-38342 None:intergenic 15.0%
!!! ATTTTCATTATGAGTTTTAT+AGG + tig0025883:38546-38565 Msa1425680:intron 15.0%
!! TAATATTTGATGTTACTCAA+TGG + tig0025883:36993-37012 Msa1425680:intron 20.0%
!! CTAAATTGTTTATTCAAACA+GGG - tig0025883:37250-37269 None:intergenic 20.0%
!! ACTAAATTGTTTATTCAAAC+AGG - tig0025883:37251-37270 None:intergenic 20.0%
!!! CACTCAAAATCATTTTTTTA+CGG - tig0025883:37289-37308 None:intergenic 20.0%
!!! AGTTAAATGGATAAACTTTT+CGG - tig0025883:38721-38740 None:intergenic 20.0%
!! AATCTATGTTCATAAATGTT+TGG + tig0025883:39517-39536 Msa1425680:three_prime_UTR 20.0%
!! GAAAAATTGTAAGATTATTG+TGG + tig0025883:39541-39560 Msa1425680:three_prime_UTR 20.0%
!! AAGTTATTGAAAAAAAATGC+TGG + tig0025883:39636-39655 Msa1425680:three_prime_UTR 20.0%
!! ATTGGGTATTGTATATATAG+AGG + tig0025883:36402-36421 Msa1425680:five_prime_UTR 25.0%
!! ATATAATTGAAAGATGGGTT+CGG - tig0025883:36491-36510 None:intergenic 25.0%
! TAGTAAAGTCACAGATTAAT+GGG - tig0025883:36917-36936 None:intergenic 25.0%
! TTAGTAAAGTCACAGATTAA+TGG - tig0025883:36918-36937 None:intergenic 25.0%
!! TTTTATTCTCTATGCATTGT+TGG + tig0025883:37166-37185 Msa1425680:intron 25.0%
! TTTATTCTCTATGCATTGTT+GGG + tig0025883:37167-37186 Msa1425680:intron 25.0%
!! TTTTTCCATTGAATGAGTAA+TGG - tig0025883:37217-37236 None:intergenic 25.0%
! ATTCAATGGAAAAACTACTT+AGG + tig0025883:37223-37242 Msa1425680:intron 25.0%
! TTCAATGGAAAAACTACTTA+GGG + tig0025883:37224-37243 Msa1425680:intron 25.0%
! TATTTGATATCTTGATGTGA+AGG + tig0025883:37319-37338 Msa1425680:intron 25.0%
!! CTTCAAGTAGAAACAATTTT+GGG + tig0025883:37404-37423 Msa1425680:intron 25.0%
!! TTCAAGTAGAAACAATTTTG+GGG + tig0025883:37405-37424 Msa1425680:intron 25.0%
! CAAAATCAATTATACTCTAG+AGG + tig0025883:37429-37448 Msa1425680:intron 25.0%
! CATAAGTGCTTATACTAAAA+GGG - tig0025883:37547-37566 None:intergenic 25.0%
! TCATAAGTGCTTATACTAAA+AGG - tig0025883:37548-37567 None:intergenic 25.0%
!!! ATCTTTAAGCTTTTGGTTTT+TGG + tig0025883:37856-37875 Msa1425680:intron 25.0%
! AAATAATACAGACAGGAAAA+TGG - tig0025883:38046-38065 None:intergenic 25.0%
! TTATTTATTGCAGAAGAGTT+AGG + tig0025883:38060-38079 Msa1425680:intron 25.0%
!! TTTAGAAGCTAATTCAAGTT+GGG + tig0025883:38418-38437 Msa1425680:intron 25.0%
! TCCTTACGATTTATAAGTTA+AGG - tig0025883:38444-38463 None:intergenic 25.0%
! GTTGTTAGTTAAAATACTCA+TGG + tig0025883:38649-38668 Msa1425680:intron 25.0%
! TTTATCCATTTAACTCTAAG+AGG + tig0025883:38726-38745 Msa1425680:intron 25.0%
! TTATACAATGACTAACATTG+TGG - tig0025883:38762-38781 None:intergenic 25.0%
! TAATAGATTCACAATAAGTC+CGG + tig0025883:38779-38798 Msa1425680:intron 25.0%
! TAACTATGATCAAATCAGTA+TGG - tig0025883:39463-39482 None:intergenic 25.0%
! AAGACTTTATGCTTATGTAA+TGG - tig0025883:39662-39681 None:intergenic 25.0%
!!! ACAATATTTTTTTTTGTCGG+AGG - tig0025883:39790-39809 None:intergenic 25.0%
TATGGCGAAAAAGAGAAAAT+CGG + tig0025883:36553-36572 Msa1425680:exon 30.0%
TAAAAGAAGAAGAAGAAGGA+AGG - tig0025883:36724-36743 None:intergenic 30.0%
TAAAATGCAAGAACGAAATC+TGG - tig0025883:36880-36899 None:intergenic 30.0%
! AGATTTCGTTCTTGCATTTT+AGG + tig0025883:36879-36898 Msa1425680:intron 30.0%
! GATTTCGTTCTTGCATTTTA+GGG + tig0025883:36880-36899 Msa1425680:intron 30.0%
TTATAAACAAAATTAGGGGC+TGG - tig0025883:36976-36995 None:intergenic 30.0%
ATTCTTAGACAACAACAAGA+AGG + tig0025883:37097-37116 Msa1425680:CDS 30.0%
! GCTTCAAGTAGAAACAATTT+TGG + tig0025883:37403-37422 Msa1425680:intron 30.0%
! TCAAGTAGAAACAATTTTGG+GGG + tig0025883:37406-37425 Msa1425680:intron 30.0%
AGAGTTGAATTTGACATGTT+TGG - tig0025883:37457-37476 None:intergenic 30.0%
GCTACAAATAAGTGTATGTT+TGG - tig0025883:37519-37538 None:intergenic 30.0%
AAACTATCCTGAAAAACTCA+TGG + tig0025883:37638-37657 Msa1425680:intron 30.0%
! CTTATTTCCATGAGTTTTTC+AGG - tig0025883:37648-37667 None:intergenic 30.0%
TAAGCATTTGTGACATTGTT+CGG - tig0025883:37718-37737 None:intergenic 30.0%
TATCATTGCCTATAGCTTAA+AGG + tig0025883:37785-37804 Msa1425680:intron 30.0%
! AGTGTGAAACTGTTATTTAG+TGG + tig0025883:37828-37847 Msa1425680:intron 30.0%
! GGACATTATCTTTAAGCTTT+TGG + tig0025883:37849-37868 Msa1425680:intron 30.0%
! GGTTTATCAACCTTTTACTT+GGG - tig0025883:37940-37959 None:intergenic 30.0%
!!! TTTTGGTGTTAGATTAGTCA+TGG + tig0025883:37999-38018 Msa1425680:intron 30.0%
CTGCAATAAATAATACAGAC+AGG - tig0025883:38053-38072 None:intergenic 30.0%
! CTTTAGAAGCTAATTCAAGT+TGG + tig0025883:38417-38436 Msa1425680:intron 30.0%
GCCTTAACTTATAAATCGTA+AGG + tig0025883:38440-38459 Msa1425680:intron 30.0%
!! GAAGTCCTTATGAGTTTTAT+AGG + tig0025883:38462-38481 Msa1425680:intron 30.0%
ACACACCTATAAAACTCATA+AGG - tig0025883:38470-38489 None:intergenic 30.0%
ACACATACTGATAATAAGCA+AGG - tig0025883:38695-38714 None:intergenic 30.0%
ATCAACCTCTTAGAGTTAAA+TGG - tig0025883:38734-38753 None:intergenic 30.0%
! CCTTTTATTGAATTTTGCCT+GGG + tig0025883:38802-38821 Msa1425680:intron 30.0%
AGCATTAGAGCAAAATCTTA+TGG - tig0025883:38844-38863 None:intergenic 30.0%
TATAGTTCAACCATCAAGAT+CGG - tig0025883:38877-38896 None:intergenic 30.0%
ACATAATATGTGGGAAACTA+CGG - tig0025883:38943-38962 None:intergenic 30.0%
AACTGTAGGACATAATATGT+GGG - tig0025883:38952-38971 None:intergenic 30.0%
TAACTGTAGGACATAATATG+TGG - tig0025883:38953-38972 None:intergenic 30.0%
TGTGCAATAATGTTAACTGT+AGG - tig0025883:38966-38985 None:intergenic 30.0%
ATGTAGAATGAGCTTGAATA+TGG + tig0025883:39064-39083 Msa1425680:intron 30.0%
!! GTTAGTTTGAATTGCTGTTT+GGG - tig0025883:39137-39156 None:intergenic 30.0%
! AGTTAGTTTGAATTGCTGTT+TGG - tig0025883:39138-39157 None:intergenic 30.0%
TACTGAGTTCTTTACTTGAT+CGG - tig0025883:39230-39249 None:intergenic 30.0%
ATACACCATTTCATTGTTAC+GGG - tig0025883:39356-39375 None:intergenic 30.0%
GATACACCATTTCATTGTTA+CGG - tig0025883:39357-39376 None:intergenic 30.0%
TGATTTGATCATAGTTAGAG+TGG + tig0025883:39466-39485 Msa1425680:exon 30.0%
GATTTGATCATAGTTAGAGT+GGG + tig0025883:39467-39486 Msa1425680:exon 30.0%
AACAAAGTATACTCTCATGT+TGG - tig0025883:39614-39633 None:intergenic 30.0%
TAAGCATAAAGTCTTACTAG+TGG + tig0025883:39667-39686 Msa1425680:three_prime_UTR 30.0%
AAGCATAAAGTCTTACTAGT+GGG + tig0025883:39668-39687 Msa1425680:three_prime_UTR 30.0%
TATTTGCTTCAGATTTCTCA+AGG + tig0025883:39694-39713 Msa1425680:three_prime_UTR 30.0%
! ATAAGAGTGTGTTAATGTGA+GGG - tig0025883:39847-39866 None:intergenic 30.0%
! AATAAGAGTGTGTTAATGTG+AGG - tig0025883:39848-39867 None:intergenic 30.0%
! GGGTATTGTATATATAGAGG+AGG + tig0025883:36405-36424 Msa1425680:five_prime_UTR 35.0%
!!! TTTTTGTTGATTCATGGCGT+GGG - tig0025883:36660-36679 None:intergenic 35.0%
!!! GGAGAGTTTTTGTTGATTCA+TGG - tig0025883:36666-36685 None:intergenic 35.0%
!! TGGTGTTTTTAATCCATTGC+AGG + tig0025883:37057-37076 Msa1425680:intron 35.0%
! TGATACATTTTCTCCTGCAA+TGG - tig0025883:37073-37092 None:intergenic 35.0%
! ATGCATTGTTGGGTTCTTTT+TGG + tig0025883:37177-37196 Msa1425680:intron 35.0%
! TGCATTGTTGGGTTCTTTTT+GGG + tig0025883:37178-37197 Msa1425680:intron 35.0%
CTGAACCATTACTCATTCAA+TGG + tig0025883:37209-37228 Msa1425680:intron 35.0%
! GGAGGCAAAATTGATTCAAT+AGG - tig0025883:37483-37502 None:intergenic 35.0%
!! TGTATGTTTGGTTCCACTTT+TGG - tig0025883:37507-37526 None:intergenic 35.0%
!! ACATGCTCATGAGCTATTTT+CGG - tig0025883:37671-37690 None:intergenic 35.0%
AAGCATTTGTGACATTGTTC+GGG - tig0025883:37717-37736 None:intergenic 35.0%
! ACTCGTTTGAGTTTATCTAC+TGG - tig0025883:37743-37762 None:intergenic 35.0%
CAAAGTGACCTTTAAGCTAT+AGG - tig0025883:37796-37815 None:intergenic 35.0%
AGTTTCACACTCAAAGCAAT+CGG - tig0025883:37819-37838 None:intergenic 35.0%
!!! TTTTGGTTTTTGGTAAGCCA+AGG + tig0025883:37866-37885 Msa1425680:intron 35.0%
! GGGTTTATCAACCTTTTACT+TGG - tig0025883:37941-37960 None:intergenic 35.0%
GGCACAAGTGATAGATATTT+GGG - tig0025883:37961-37980 None:intergenic 35.0%
TGGCACAAGTGATAGATATT+TGG - tig0025883:37962-37981 None:intergenic 35.0%
! TGGTCTAGCTTCTAACTAAT+AGG + tig0025883:38138-38157 Msa1425680:intron 35.0%
! GTTCTAACGTAAGTTGAAAC+TGG + tig0025883:38255-38274 Msa1425680:intron 35.0%
!! CTTCTAAAGCTCAAACACAT+GGG - tig0025883:38406-38425 None:intergenic 35.0%
GGCAAAATTCAATAAAAGGC+CGG - tig0025883:38801-38820 None:intergenic 35.0%
! GCCTTTTATTGAATTTTGCC+TGG + tig0025883:38801-38820 Msa1425680:intron 35.0%
CCCAGGCAAAATTCAATAAA+AGG - tig0025883:38805-38824 None:intergenic 35.0%
! TTTATTGAATTTTGCCTGGG+TGG + tig0025883:38805-38824 Msa1425680:intron 35.0%
! TTATTGAATTTTGCCTGGGT+GGG + tig0025883:38806-38825 Msa1425680:intron 35.0%
TATATCTACATCCGGATATC+AGG - tig0025883:39024-39043 None:intergenic 35.0%
CCAAAGGTTATATCTACATC+CGG - tig0025883:39032-39051 None:intergenic 35.0%
AACAGTACTTCCAAATGCAT+TGG - tig0025883:39179-39198 None:intergenic 35.0%
TTCGAGTAATGTCATGTCAT+CGG + tig0025883:39321-39340 Msa1425680:CDS 35.0%
AATGAAATGGTGTATCCTAG+TGG + tig0025883:39361-39380 Msa1425680:CDS 35.0%
ACGAATTTGAATCTCTGTTC+TGG - tig0025883:39387-39406 None:intergenic 35.0%
AGCTTCAAACTGGAAAACAA+TGG - tig0025883:39590-39609 None:intergenic 35.0%
AAGAGAAGACTGGAACAATG+GGG - tig0025883:36438-36457 None:intergenic 40.0%
GAAGAGAAGACTGGAACAAT+GGG - tig0025883:36439-36458 None:intergenic 40.0%
AGAAGAGAAGACTGGAACAA+TGG - tig0025883:36440-36459 None:intergenic 40.0%
GAAGTAAGAGAAGAGAAGAC+TGG - tig0025883:36448-36467 None:intergenic 40.0%
!!! GTTTTTGTTGATTCATGGCG+TGG - tig0025883:36661-36680 None:intergenic 40.0%
CAAAAACTCTCCTTTCAAGC+CGG + tig0025883:36674-36693 Msa1425680:CDS 40.0%
GGGGTAAAAGAAGAAGAAGA+AGG - tig0025883:36728-36747 None:intergenic 40.0%
TGCAGGAGAAAATGTATCAG+TGG + tig0025883:37074-37093 Msa1425680:intron 40.0%
TGATATAGTCTCTCACTTGC+AGG + tig0025883:37138-37157 Msa1425680:CDS 40.0%
! AATTTTGCCTCCTCCAAAAG+TGG + tig0025883:37491-37510 Msa1425680:intron 40.0%
!! ATGTTTGGTTCCACTTTTGG+AGG - tig0025883:37504-37523 None:intergenic 40.0%
TATAGGCAATGATAGACCTG+GGG - tig0025883:37779-37798 None:intergenic 40.0%
CTATAGGCAATGATAGACCT+GGG - tig0025883:37780-37799 None:intergenic 40.0%
GGATCAAACTCCCAAGTAAA+AGG + tig0025883:37927-37946 Msa1425680:intron 40.0%
! CACCAAAAGTATAACCGACA+CGG - tig0025883:37987-38006 None:intergenic 40.0%
AGAGTTAGGTCCTATGTGAA+AGG + tig0025883:38074-38093 Msa1425680:intron 40.0%
GAGTTAGGTCCTATGTGAAA+GGG + tig0025883:38075-38094 Msa1425680:intron 40.0%
AGTTAGAAGCTAGACCAAAC+TGG - tig0025883:38135-38154 None:intergenic 40.0%
!! GCTTCTAAAGCTCAAACACA+TGG - tig0025883:38407-38426 None:intergenic 40.0%
ATGTATCAGTCCGATCTTGA+TGG + tig0025883:38864-38883 Msa1425680:intron 40.0%
! ACAACTTTTCTCGCTTCACT+TGG + tig0025883:38916-38935 Msa1425680:intron 40.0%
TTCGAATGTCTCCTGATATC+CGG + tig0025883:39010-39029 Msa1425680:intron 40.0%
CCGGATGTAGATATAACCTT+TGG + tig0025883:39029-39048 Msa1425680:intron 40.0%
ACATGCAAACACATAGCCAA+AGG - tig0025883:39048-39067 None:intergenic 40.0%
TTCTGTTGAATTGACTGCCT+TGG - tig0025883:39112-39131 None:intergenic 40.0%
GTACTTCCAAATGCATTGGA+CGG - tig0025883:39175-39194 None:intergenic 40.0%
GTGCTAAATGATATGGCTGA+AGG - tig0025883:39288-39307 None:intergenic 40.0%
TCAGCCATATCATTTAGCAC+AGG + tig0025883:39288-39307 Msa1425680:CDS 40.0%
CAGCCATATCATTTAGCACA+GGG + tig0025883:39289-39308 Msa1425680:CDS 40.0%
GATGACATGACATTACTCGA+AGG - tig0025883:39322-39341 None:intergenic 40.0%
TCGAGTAATGTCATGTCATC+GGG + tig0025883:39322-39341 Msa1425680:CDS 40.0%
CAGTATGGAAAATCATGACC+AGG - tig0025883:39448-39467 None:intergenic 40.0%
AGTGGGTAACAGTAAAGAGA+AGG + tig0025883:39484-39503 Msa1425680:three_prime_UTR 40.0%
TCATGTTGGAAGCTTCAAAC+TGG - tig0025883:39600-39619 None:intergenic 40.0%
CTTCAGATTTCTCAAGGCTT+AGG + tig0025883:39700-39719 Msa1425680:three_prime_UTR 40.0%
AGAGAAGACTGGAACAATGG+GGG - tig0025883:36437-36456 None:intergenic 45.0%
TCTTCTCTTACTTCTCTGCC+CGG + tig0025883:36454-36473 Msa1425680:five_prime_UTR 45.0%
!! ATTGAAAGATGGGTTCGGTG+CGG - tig0025883:36486-36505 None:intergenic 45.0%
ACCAATCCTCCTTCCTTGAT+CGG + tig0025883:36524-36543 Msa1425680:five_prime_UTR 45.0%
ATATCTCCACCGATCAAGGA+AGG - tig0025883:36536-36555 None:intergenic 45.0%
TTCCTTGATCGGTGGAGATA+TGG + tig0025883:36535-36554 Msa1425680:exon 45.0%
GGCGAAAAAGAGAAAATCGG+TGG + tig0025883:36556-36575 Msa1425680:CDS 45.0%
GAGATGAGAGAGGGAATTAG+CGG - tig0025883:36633-36652 None:intergenic 45.0%
GTGGGTATAGAGATGAGAGA+GGG - tig0025883:36642-36661 None:intergenic 45.0%
CAAAATTAGGGGCTGGAATG+TGG - tig0025883:36969-36988 None:intergenic 45.0%
!! TTTGGTTCCACTTTTGGAGG+AGG - tig0025883:37501-37520 None:intergenic 45.0%
TCAAACGAGTCAGTCCAAAC+AGG + tig0025883:37753-37772 Msa1425680:intron 45.0%
GCTATAGGCAATGATAGACC+TGG - tig0025883:37781-37800 None:intergenic 45.0%
AAAGTATAACCGACACGGTG+TGG - tig0025883:37982-38001 None:intergenic 45.0%
!!! CACCGTGTCGGTTATACTTT+TGG + tig0025883:37982-38001 Msa1425680:intron 45.0%
TTAGGTCCTATGTGAAAGGG+AGG + tig0025883:38078-38097 Msa1425680:intron 45.0%
TAGGTCCTATGTGAAAGGGA+GGG + tig0025883:38079-38098 Msa1425680:intron 45.0%
AGTGCAGCTTAAGTCCAGTT+TGG + tig0025883:38118-38137 Msa1425680:intron 45.0%
! TTGCCTGGGTGGGATTTTTA+TGG + tig0025883:38816-38835 Msa1425680:intron 45.0%
TGAGCTTGAATATGGAGCAG+AGG + tig0025883:39072-39091 Msa1425680:CDS 45.0%
AGGAATCACCAGTTTCTCCA+AGG + tig0025883:39092-39111 Msa1425680:CDS 45.0%
ATTGACTGCCTTGGAGAAAC+TGG - tig0025883:39103-39122 None:intergenic 45.0%
TCCATACCGTCCAATGCATT+TGG + tig0025883:39166-39185 Msa1425680:CDS 45.0%
TCCAAATGCATTGGACGGTA+TGG - tig0025883:39170-39189 None:intergenic 45.0%
!! GCATTTGGAAGTACTGTTGC+AGG + tig0025883:39181-39200 Msa1425680:CDS 45.0%
CTGCCCTGTGCTAAATGATA+TGG - tig0025883:39295-39314 None:intergenic 45.0%
CGAGTAATGTCATGTCATCG+GGG + tig0025883:39323-39342 Msa1425680:CDS 45.0%
AATGTCATGTCATCGGGGAA+CGG + tig0025883:39328-39347 Msa1425680:CDS 45.0%
CGGAACCCGTAACAATGAAA+TGG + tig0025883:39348-39367 Msa1425680:CDS 45.0%
ATGCATGTCCATGCAATCAC+TGG - tig0025883:39416-39435 None:intergenic 45.0%
!! AAATATTATAAACAAAATTA+GGG - tig0025883:36981-37000 None:intergenic 5.0%
ACCGATCAAGGAAGGAGGAT+TGG - tig0025883:36528-36547 None:intergenic 50.0%
AATCCTCCTTCCTTGATCGG+TGG + tig0025883:36527-36546 Msa1425680:five_prime_UTR 50.0%
ATGAGAGAGGGAATTAGCGG+TGG - tig0025883:36630-36649 None:intergenic 50.0%
CGTGGGTATAGAGATGAGAG+AGG - tig0025883:36643-36662 None:intergenic 50.0%
! GAAGGAAGCATACAAGAGCG+TGG - tig0025883:36706-36725 None:intergenic 50.0%
ACTTGGGAGTTTGATCCCGA+GGG - tig0025883:37924-37943 None:intergenic 50.0%
TACTTGGGAGTTTGATCCCG+AGG - tig0025883:37925-37944 None:intergenic 50.0%
AGGTCCTATGTGAAAGGGAG+GGG + tig0025883:38080-38099 Msa1425680:intron 50.0%
GTGCCATAAAAATCCCACCC+AGG - tig0025883:38822-38841 None:intergenic 50.0%
TGCATTGGACGGTATGGATG+CGG - tig0025883:39164-39183 None:intergenic 50.0%
!! TGGAAGTACTGTTGCAGGAC+AGG + tig0025883:39186-39205 Msa1425680:CDS 50.0%
!! GGAAGTACTGTTGCAGGACA+GGG + tig0025883:39187-39206 Msa1425680:CDS 50.0%
!! GAAGTACTGTTGCAGGACAG+GGG + tig0025883:39188-39207 Msa1425680:CDS 50.0%
GAATCTCTGTTCTGGCCACT+AGG - tig0025883:39379-39398 None:intergenic 50.0%
GTCCAACTCCAGTGATTGCA+TGG + tig0025883:39405-39424 Msa1425680:CDS 50.0%
GTCCATGCAATCACTGGAGT+TGG - tig0025883:39410-39429 None:intergenic 50.0%
TCTCCACCGATCAAGGAAGG+AGG - tig0025883:36533-36552 None:intergenic 55.0%
CGCCATATCTCCACCGATCA+AGG - tig0025883:36540-36559 None:intergenic 55.0%
GGAGTCTCGTCTCGATGAAG+TGG + tig0025883:36577-36596 Msa1425680:CDS 55.0%
GGTGCAGAAGGTGGAGTACA+TGG - tig0025883:36609-36628 None:intergenic 55.0%
ATTAGCGGTGGTGCAGAAGG+TGG - tig0025883:36618-36637 None:intergenic 55.0%
GGAATTAGCGGTGGTGCAGA+AGG - tig0025883:36621-36640 None:intergenic 55.0%
! TACAAGAGCGTGGCGTTCAC+CGG - tig0025883:36696-36715 None:intergenic 55.0%
TGATAGACCTGGGGCCTGTT+TGG - tig0025883:37770-37789 None:intergenic 55.0%
! CAAGGTATCATCCCCTCGCA+CGG + tig0025883:37884-37903 Msa1425680:intron 55.0%
GGCTGGGGAGATTAATCCCT+CGG + tig0025883:37905-37924 Msa1425680:intron 55.0%
GCTGGGGAGATTAATCCCTC+GGG + tig0025883:37906-37925 Msa1425680:intron 55.0%
TCACTTGTGCCACACCGTGT+CGG + tig0025883:37970-37989 Msa1425680:intron 55.0%
GGTCCTATGTGAAAGGGAGG+GGG + tig0025883:38081-38100 Msa1425680:intron 55.0%
AAGCCCCCTCCCTTTCACAT+AGG - tig0025883:38087-38106 None:intergenic 55.0%
TATGTGAAAGGGAGGGGGCT+TGG + tig0025883:38086-38105 Msa1425680:intron 55.0%
CAGGACAGGGGATACGAACT+GGG + tig0025883:39200-39219 Msa1425680:CDS 55.0%
GACATGCATGCAGACAGTCC+TGG + tig0025883:39427-39446 Msa1425680:CDS 55.0%
!! GTGGCGTTCACCGGCTTGAA+AGG - tig0025883:36687-36706 None:intergenic 60.0%
AGTCAGTCCAAACAGGCCCC+AGG + tig0025883:37760-37779 Msa1425680:intron 60.0%
CGTGCGAGGGGATGATACCT+TGG - tig0025883:37886-37905 None:intergenic 60.0%
TATCATCCCCTCGCACGGCT+GGG + tig0025883:37889-37908 Msa1425680:intron 60.0%
TAATCTCCCCAGCCGTGCGA+GGG - tig0025883:37899-37918 None:intergenic 60.0%
TTAATCTCCCCAGCCGTGCG+AGG - tig0025883:37900-37919 None:intergenic 60.0%
GCAGGACAGGGGATACGAAC+TGG + tig0025883:39199-39218 Msa1425680:CDS 60.0%
GCTGAAGGCTGCGACGAATG+GGG - tig0025883:39273-39292 None:intergenic 60.0%
GGCTGAAGGCTGCGACGAAT+GGG - tig0025883:39274-39293 None:intergenic 60.0%
TGGCTGAAGGCTGCGACGAA+TGG - tig0025883:39275-39294 None:intergenic 60.0%
GTATCATCCCCTCGCACGGC+TGG + tig0025883:37888-37907 Msa1425680:intron 65.0%
ATCATCCCCTCGCACGGCTG+GGG + tig0025883:37890-37909 Msa1425680:intron 65.0%
AATCTCCCCAGCCGTGCGAG+GGG - tig0025883:37898-37917 None:intergenic 65.0%
!! GGTTCGGTGCGGTGCTTGCC+GGG - tig0025883:36475-36494 None:intergenic 70.0%
!! GGGTTCGGTGCGGTGCTTGC+CGG - tig0025883:36476-36495 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0025883 gene 36402 39870 36402 ID=Msa1425680;Name=Msa1425680
tig0025883 mRNA 36402 39870 36402 ID=Msa1425680-mRNA-1;Parent=Msa1425680;Name=Msa1425680-mRNA-1;_AED=0.00;_eAED=0.00;_QI=152|1|1|1|1|1|3|402|212
tig0025883 exon 36402 36712 36402 ID=Msa1425680-mRNA-1:exon:4395;Parent=Msa1425680-mRNA-1
tig0025883 exon 37079 37159 37079 ID=Msa1425680-mRNA-1:exon:4396;Parent=Msa1425680-mRNA-1
tig0025883 exon 39070 39870 39070 ID=Msa1425680-mRNA-1:exon:4397;Parent=Msa1425680-mRNA-1
tig0025883 five_prime_UTR 36402 36553 36402 ID=Msa1425680-mRNA-1:five_prime_utr;Parent=Msa1425680-mRNA-1
tig0025883 CDS 36554 36712 36554 ID=Msa1425680-mRNA-1:cds;Parent=Msa1425680-mRNA-1
tig0025883 CDS 37079 37159 37079 ID=Msa1425680-mRNA-1:cds;Parent=Msa1425680-mRNA-1
tig0025883 CDS 39070 39468 39070 ID=Msa1425680-mRNA-1:cds;Parent=Msa1425680-mRNA-1
tig0025883 three_prime_UTR 39469 39870 39469 ID=Msa1425680-mRNA-1:three_prime_utr;Parent=Msa1425680-mRNA-1
Gene Sequence

>Msa1425680

ATGGCGAAAAAGAGAAAATCGGTGGAGTCTCGTCTCGATGAAGTGGATCGCACCATGTACTCCACCTTCTGCACCACCGCTAATTCCCTCTCTCATCTCTATACCCACGCCATGAATCAACAAAAACTCTCCTTTCAAGCCGGTGAACGCCACGCTCTTGAGAAAATGTATCAGTGGATTCTTAGACAACAACAAGAAGGAATGAGAGTCACTACTATTGATATAGTCTCTCACTTGCAGAATGAGCTTGAATATGGAGCAGAGGAATCACCAGTTTCTCCAAGGCAGTCAATTCAACAGAACTCCCAAACAGCAATTCAAACTAACTTTGCCGCATCCATACCGTCCAATGCATTTGGAAGTACTGTTGCAGGACAGGGGATACGAACTGGGCAAACCGATCAAGTAAAGAACTCAGTATTCTCAAATGCACTCTCTAGCCCCATTCGTCGCAGCCTTCAGCCATATCATTTAGCACAGGGCAGCAATCCTTCGAGTAATGTCATGTCATCGGGGAACGGAACCCGTAACAATGAAATGGTGTATCCTAGTGGCCAGAACAGAGATTCAAATTCGTCCAACTCCAGTGATTGCATGGACATGCATGCAGACAGTCCTGGTCATGATTTTCCATACTGA
Protein sequence

>Msa1425680

MAKKRKSVESRLDEVDRTMYSTFCTTANSLSHLYTHAMNQQKLSFQAGERHALEKMYQWILRQQQEGMRVTTIDIVSHLQNELEYGAEESPVSPRQSIQQNSQTAIQTNFAASIPSNAFGSTVAGQGIRTGQTDQVKNSVFSNALSSPIRRSLQPYHLAQGSNPSSNVMSSGNGTRNNEMVYPSGQNRDSNSSNSSDCMDMHADSPGHDFPY*