AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1416420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1416420 MtrunA17_Chr1g0193671 95.434 219 10 0 1 219 1 219 6.60e-153 423
Msa1416420 MtrunA17_Chr1g0193421 71.000 200 58 0 19 218 20 219 2.96e-100 290
Msa1416420 MtrunA17_Chr7g0271211 69.388 196 60 0 22 217 19 214 1.18e-94 275
Msa1416420 MtrunA17_Chr1g0189841 46.479 213 108 3 6 213 5 216 3.77e-60 188
Msa1416420 MtrunA17_Chr2g0285871 47.596 208 102 3 11 213 10 215 5.23e-60 187
Msa1416420 MtrunA17_Chr4g0007041 47.115 208 103 3 11 213 10 215 7.17e-60 187
Msa1416420 MtrunA17_Chr4g0007051 47.115 208 103 3 11 213 10 215 7.99e-60 187
Msa1416420 MtrunA17_Chr4g0007031 47.115 208 103 3 11 213 10 215 9.01e-60 187
Msa1416420 MtrunA17_Chr5g0419971 47.115 208 103 3 11 213 10 215 9.01e-60 187
Msa1416420 MtrunA17_Chr5g0419981 47.115 208 103 3 11 213 10 215 9.01e-60 187
Msa1416420 MtrunA17_Chr6g0449751 47.393 211 104 4 8 213 6 214 5.05e-58 182
Msa1416420 MtrunA17_Chr6g0449771 48.259 201 98 3 17 213 16 214 1.34e-57 181
Msa1416420 MtrunA17_Chr6g0449761 48.259 201 98 3 17 213 16 214 2.35e-57 181
Msa1416420 MtrunA17_Chr6g0449731 48.259 201 98 3 17 213 16 214 3.61e-57 180
Msa1416420 MtrunA17_Chr6g0449791 49.010 202 95 4 8 203 6 205 3.39e-56 177
Msa1416420 MtrunA17_Chr1g0156131 43.415 205 108 2 11 213 10 208 4.13e-56 177
Msa1416420 MtrunA17_Chr2g0292831 45.408 196 102 2 20 213 19 211 1.33e-55 176
Msa1416420 MtrunA17_Chr1g0210761 42.381 210 114 3 7 216 7 209 1.83e-54 173
Msa1416420 MtrunA17_Chr2g0292901 43.719 199 106 3 18 213 17 212 4.82e-52 167
Msa1416420 MtrunA17_Chr6g0449781 43.128 211 112 5 8 213 6 213 3.24e-51 165
Msa1416420 MtrunA17_Chr5g0414971 43.590 195 109 1 22 215 26 220 1.03e-49 161
Msa1416420 MtrunA17_Chr1g0178711 38.009 221 126 5 6 218 8 225 1.39e-48 159
Msa1416420 MtrunA17_Chr2g0292861 44.444 171 92 2 46 213 7 177 5.81e-46 150
Msa1416420 MtrunA17_Chr2g0292841 44.444 171 92 2 46 213 7 177 3.05e-45 148
Msa1416420 MtrunA17_Chr8g0335851 43.243 185 101 3 6 188 10 192 8.10e-41 138
Msa1416420 MtrunA17_Chr8g0343911 38.378 185 110 1 8 192 4 184 2.16e-38 132
Msa1416420 MtrunA17_Chr2g0321601 39.899 198 95 6 19 211 22 200 3.41e-38 131
Msa1416420 MtrunA17_Chr2g0321581 39.899 198 95 6 19 211 23 201 3.78e-38 131
Msa1416420 MtrunA17_Chr2g0321591 39.899 198 95 6 19 211 23 201 5.28e-38 130
Msa1416420 MtrunA17_Chr3g0130241 38.350 206 121 4 9 210 73 276 1.62e-37 132
Msa1416420 MtrunA17_Chr8g0343954 35.417 192 120 3 26 217 21 208 2.13e-36 127
Msa1416420 MtrunA17_Chr8g0343934 34.673 199 126 3 19 217 14 208 2.50e-36 127
Msa1416420 MtrunA17_Chr2g0292911 52.137 117 54 1 46 160 7 123 5.75e-36 124
Msa1416420 MtrunA17_Chr2g0313961 37.255 204 124 3 11 214 6 205 2.42e-35 124
Msa1416420 MtrunA17_Chr2g0297241 37.895 190 114 3 26 215 21 206 3.30e-35 124
Msa1416420 MtrunA17_Chr3g0084511 36.842 190 116 3 26 215 20 205 6.68e-35 123
Msa1416420 MtrunA17_Chr4g0026191 43.284 134 73 2 79 211 71 202 1.03e-34 122
Msa1416420 MtrunA17_Chr2g0302171 37.019 208 127 3 8 215 3 206 1.16e-34 122
Msa1416420 MtrunA17_Chr4g0003361 39.423 208 108 8 9 213 8 200 1.25e-34 122
Msa1416420 MtrunA17_Chr4g0026201 43.284 134 73 2 79 211 71 202 1.32e-34 122
Msa1416420 MtrunA17_Chr2g0297231 36.058 208 129 3 8 215 3 206 1.65e-34 122
Msa1416420 MtrunA17_Chr8g0343961 32.381 210 134 4 8 217 7 208 4.39e-34 120
Msa1416420 MtrunA17_Chr3g0084521 36.842 190 116 3 26 215 20 205 6.78e-34 120
Msa1416420 MtrunA17_Chr5g0411961 33.175 211 131 4 8 215 3 206 8.27e-34 120
Msa1416420 MtrunA17_Chr3g0084531 36.000 200 118 4 19 215 14 206 1.02e-33 120
Msa1416420 MtrunA17_Chr2g0300561 37.097 186 109 3 29 211 23 203 2.11e-33 119
Msa1416420 MtrunA17_Chr2g0300581 34.783 207 128 3 6 212 5 204 6.36e-33 118
Msa1416420 MtrunA17_Chr2g0300591 34.951 206 127 3 6 211 5 203 9.66e-33 117
Msa1416420 MtrunA17_Chr2g0301611 34.615 208 128 4 8 215 7 206 2.08e-32 116
Msa1416420 MtrunA17_Chr8g0344041 35.437 206 122 6 11 213 11 208 3.92e-31 113
Msa1416420 MtrunA17_Chr8g0344051 41.420 169 90 4 8 173 7 169 1.50e-30 112
Msa1416420 MtrunA17_Chr2g0302541 35.789 190 118 3 26 215 21 206 2.13e-30 111
Msa1416420 MtrunA17_Chr4g0012651 35.979 189 117 4 27 215 22 206 4.46e-30 110
Msa1416420 MtrunA17_Chr7g0217811 35.979 189 117 4 27 215 22 206 5.76e-30 110
Msa1416420 MtrunA17_Chr8g0344021 36.275 204 121 4 9 211 10 205 1.10e-29 109
Msa1416420 MtrunA17_Chr8g0343921 32.039 206 132 3 6 211 3 200 5.48e-28 105
Msa1416420 MtrunA17_Chr2g0286171 32.227 211 134 6 5 213 4 207 1.72e-27 103
Msa1416420 MtrunA17_Chr8g0344031 34.343 198 120 6 19 213 16 206 2.38e-26 100
Msa1416420 MtrunA17_Chr3g0084551 33.171 205 125 6 8 211 3 196 2.25e-25 99.0
Msa1416420 MtrunA17_Chr3g0115141 33.155 187 117 5 28 213 26 205 6.61e-25 97.4
Msa1416420 MtrunA17_Chr3g0084501 40.187 107 57 3 8 111 3 105 2.59e-15 69.3
Msa1416420 MtrunA17_Chr2g0292881 52.632 57 25 1 94 148 1 57 9.35e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1416420 AT3G62020.1 69.545 220 64 2 1 218 1 219 4.24e-108 310
Msa1416420 AT1G02335.1 64.929 211 74 0 8 218 9 219 2.01e-101 293
Msa1416420 AT1G09560.1 70.707 198 58 0 21 218 22 219 1.01e-99 288
Msa1416420 AT3G62020.2 74.317 183 44 2 38 218 9 190 5.18e-98 283
Msa1416420 AT5G26700.1 60.309 194 74 1 25 218 23 213 1.56e-86 255
Msa1416420 AT3G05930.1 53.881 219 100 1 1 218 1 219 9.79e-79 235
Msa1416420 AT1G18980.1 46.411 209 110 1 7 215 13 219 2.19e-64 199
Msa1416420 AT1G18970.1 46.890 209 109 1 7 215 13 219 1.10e-63 197
Msa1416420 AT5G38910.1 49.038 208 102 2 11 214 12 219 2.43e-62 194
Msa1416420 AT4G14630.1 50.505 198 93 2 21 213 24 221 8.41e-62 192
Msa1416420 AT5G39160.1 45.972 211 110 2 9 215 10 220 1.22e-61 192
Msa1416420 AT5G39190.1 45.972 211 110 2 9 215 10 220 2.01e-61 191
Msa1416420 AT5G39130.1 45.972 211 110 2 9 215 10 220 2.05e-61 191
Msa1416420 AT5G39150.1 46.445 211 108 2 8 213 8 218 3.15e-61 191
Msa1416420 AT5G38930.1 49.749 199 96 2 19 213 22 220 7.09e-61 190
Msa1416420 AT5G39180.1 46.445 211 108 2 8 213 8 218 9.75e-61 190
Msa1416420 AT5G39120.1 48.485 198 97 2 21 213 21 218 1.09e-60 189
Msa1416420 AT5G38940.1 49.490 196 95 2 22 213 25 220 1.38e-60 189
Msa1416420 AT3G05950.1 48.571 210 102 3 12 215 13 222 1.52e-60 189
Msa1416420 AT5G38940.2 49.490 196 95 2 22 213 49 244 2.04e-60 190
Msa1416420 AT5G39110.1 47.087 206 104 2 13 213 14 219 2.25e-60 189
Msa1416420 AT5G38960.1 48.500 200 98 3 21 215 24 223 7.43e-60 187
Msa1416420 AT3G04200.1 45.755 212 109 3 11 216 13 224 1.92e-59 187
Msa1416420 AT5G39160.3 44.286 210 111 2 9 215 10 216 3.60e-58 183
Msa1416420 AT3G04170.1 44.131 213 110 4 11 217 12 221 5.68e-52 167
Msa1416420 AT3G04180.1 43.564 202 104 4 17 211 20 218 1.24e-50 164
Msa1416420 AT5G39160.2 40.476 210 101 2 9 215 10 198 1.60e-50 163
Msa1416420 AT5G39190.2 40.476 210 101 2 9 215 10 198 2.61e-50 162
Msa1416420 AT3G04150.1 41.546 207 115 3 11 211 12 218 2.70e-50 163
Msa1416420 AT3G04190.1 42.714 199 110 2 17 211 20 218 1.42e-49 161
Msa1416420 AT3G04150.2 39.545 220 114 3 11 211 12 231 6.77e-49 160
Msa1416420 AT3G10080.1 41.379 203 111 4 19 216 22 221 1.42e-47 156
Msa1416420 AT3G10080.2 41.379 203 111 4 19 216 93 292 7.80e-47 157
Msa1416420 AT5G38910.2 41.463 205 83 2 11 213 12 181 9.21e-43 142
Msa1416420 AT1G74820.1 36.967 211 126 2 8 213 16 224 2.11e-42 143
Msa1416420 AT1G72610.1 37.097 186 115 1 26 211 18 201 2.04e-39 135
Msa1416420 AT1G10460.1 38.725 204 108 5 22 214 18 215 9.31e-39 133
Msa1416420 AT5G61750.1 40.116 172 101 1 11 180 7 178 5.64e-38 131
Msa1416420 AT5G20630.1 36.275 204 124 2 8 211 7 204 9.11e-37 128
Msa1416420 AT5G38950.1 39.744 78 45 1 143 218 2 79 1.20e-12 62.4

Find 61 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATAAATTAAGTCTTCTT+AGG 0.231546 tig0024351:+25754 None:intergenic
TGTACTTGTAACATTTATTA+TGG 0.237046 tig0024351:-25632 Msa1416420:three_prime_UTR
TGATAATGCACCAACCCTTT+TGG 0.273835 tig0024351:+25955 None:intergenic
CAACAACACTGTAGGATCTT+TGG 0.279663 tig0024351:-26196 Msa1416420:CDS
TAATCATGTCACCAACAAAT+TGG 0.290827 tig0024351:-26993 Msa1416420:exon
CAAAGGTCTTGAAGTGTATC+AGG 0.335291 tig0024351:+26919 None:intergenic
GTGCATTATCAGAAGAATAA+TGG 0.349181 tig0024351:-25943 Msa1416420:CDS
TGCATTATCAGAAGAATAAT+GGG 0.349638 tig0024351:-25942 Msa1416420:CDS
TTGCTTTGAATTTAAGTTGA+AGG 0.354144 tig0024351:-27223 Msa1416420:five_prime_UTR
CTAATACTTACCATCACATT+TGG 0.356231 tig0024351:-27192 Msa1416420:five_prime_UTR
CTTTCAGATTGATACTAAAC+AGG 0.392793 tig0024351:-25803 Msa1416420:CDS
TATGTAGGTGTTAAGGTGAA+TGG 0.396371 tig0024351:-26294 Msa1416420:intron
ACAGTGTTGTTGACAACCTT+AGG 0.398287 tig0024351:+26207 None:intergenic
CACGAGGGTGTGTGTGTGGC+GGG 0.405545 tig0024351:+26085 None:intergenic
GAAATCTTTGTGTTCCCAAA+AGG 0.405554 tig0024351:-25970 Msa1416420:CDS
TCCTGATGATGTTCTTGCTC+AGG 0.416572 tig0024351:-25827 Msa1416420:CDS
TGTGTAGCTCTTCCATCATC+AGG 0.427639 tig0024351:-26898 Msa1416420:intron
AAGAGAGAAGAAAGTTGAGT+TGG 0.430179 tig0024351:+27093 None:intergenic
TTAGTTAGTTCGTTGGTTGC+TGG 0.433772 tig0024351:+27316 None:intergenic
TGTGGCGGGTTAAGACCATT+AGG 0.441756 tig0024351:+26099 None:intergenic
TAGTTAGTTCGTTGGTTGCT+GGG 0.450959 tig0024351:+27317 None:intergenic
TAATTTATAATATTCAAGAA+AGG 0.460213 tig0024351:-25742 Msa1416420:three_prime_UTR
TTTGAAATAATAATTCTTGA+AGG 0.461623 tig0024351:-25669 Msa1416420:three_prime_UTR
TGCGTTAGATCAATAATGAA+TGG 0.462747 tig0024351:+27055 None:intergenic
AGAATAGACTACAAACCTAA+TGG 0.465489 tig0024351:-26114 Msa1416420:CDS
GCCTGAGCAAGAACATCATC+AGG 0.472294 tig0024351:+25826 None:intergenic
CAGTGTTGTTGACAACCTTA+GGG 0.477171 tig0024351:+26208 None:intergenic
CAGTCCAATGAAAAGAGGTT+TGG 0.477909 tig0024351:+27356 None:intergenic
AATTTATAATATTCAAGAAA+GGG 0.481410 tig0024351:-25741 Msa1416420:three_prime_UTR
GAGAAGAAAGTTGAGTTGGA+TGG 0.485875 tig0024351:+27097 None:intergenic
TTGAAATAATAATTCTTGAA+GGG 0.488406 tig0024351:-25668 Msa1416420:three_prime_UTR
ATTGACAGTCCAATGAAAAG+AGG 0.492580 tig0024351:+27351 None:intergenic
AAATCTTTGTGTTCCCAAAA+GGG 0.493371 tig0024351:-25969 Msa1416420:CDS
CTTTGTGTTCCCAAAAGGGT+TGG 0.498869 tig0024351:-25965 Msa1416420:CDS
CATTATACATTACCTGATGA+TGG 0.506437 tig0024351:+26886 None:intergenic
ATTCCTGGACTCAACACATT+GGG 0.515180 tig0024351:-26150 Msa1416420:CDS
GCACGAGGGTGTGTGTGTGG+CGG 0.527458 tig0024351:+26084 None:intergenic
TTATATATGGCATAAGGCAA+TGG 0.532581 tig0024351:+27146 None:intergenic
ACTCCCAATGTGTTGAGTCC+AGG 0.538199 tig0024351:+26147 None:intergenic
ATACATATTAGTTAGTTCGT+TGG 0.547017 tig0024351:+27309 None:intergenic
GTTGTTATTGATAGAGTGGA+TGG 0.548602 tig0024351:+27168 None:intergenic
TGATGGTTTAGCAAACCCTA+AGG 0.553318 tig0024351:-26223 Msa1416420:CDS
TATGTCTTATGTAGGTGTTA+AGG 0.563003 tig0024351:-26301 Msa1416420:intron
TTGGAAGGTGAATTAGATGT+AGG 0.564991 tig0024351:-26042 Msa1416420:CDS
AATTCCTGGACTCAACACAT+TGG 0.570200 tig0024351:-26151 Msa1416420:CDS
TGTATCAGGATCAGAAGCGA+AGG 0.579047 tig0024351:+26933 None:intergenic
AGAAGCGAAGGTAGATGAGA+TGG 0.579500 tig0024351:+26945 None:intergenic
GAAACAATTGACAAAGTACC+AGG 0.582389 tig0024351:+25877 None:intergenic
AATGGTTGTTATTGATAGAG+TGG 0.584311 tig0024351:+27164 None:intergenic
GGTGAATGGATTTGCATGCA+AGG 0.593738 tig0024351:-26280 Msa1416420:CDS
CTGTTTAGTATCAATCTGAA+AGG 0.609012 tig0024351:+25804 None:intergenic
TCTGTTTGACTCTACACCAA+CGG 0.619003 tig0024351:-25851 Msa1416420:CDS
GTGGCACGAGGGTGTGTGTG+TGG 0.621879 tig0024351:+26081 None:intergenic
AGAACATCATCAGGAACCGT+TGG 0.626429 tig0024351:+25835 None:intergenic
AAGGTTGTCAACAACACTGT+AGG 0.630939 tig0024351:-26204 Msa1416420:CDS
AAAACAACCTCAGTGGCACG+AGG 0.637231 tig0024351:+26069 None:intergenic
CACACACCCTCGTGCCACTG+AGG 0.678341 tig0024351:-26076 Msa1416420:CDS
AGTGGATGGACCAAATGTGA+TGG 0.697546 tig0024351:+27182 None:intergenic
AAACAACCTCAGTGGCACGA+GGG 0.703640 tig0024351:+26070 None:intergenic
GCATTATCAGAAGAATAATG+GGG 0.712368 tig0024351:-25941 Msa1416420:CDS
ATGATGGAAGAGCTACACAA+AGG 0.722041 tig0024351:+26902 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATACGATTTTATTTATATA+TGG + tig0024351:25642-25661 None:intergenic 10.0%
!! TAATTTATAATATTCAAGAA+AGG - tig0024351:27030-27049 Msa1416420:five_prime_UTR 10.0%
!! AATTTATAATATTCAAGAAA+GGG - tig0024351:27031-27050 Msa1416420:five_prime_UTR 10.0%
!!! AATACGATTTTATTTATATA+TGG + tig0024351:25642-25661 None:intergenic 10.0%
!! TAATTTATAATATTCAAGAA+AGG - tig0024351:27030-27049 Msa1416420:five_prime_UTR 10.0%
!! AATTTATAATATTCAAGAAA+GGG - tig0024351:27031-27050 Msa1416420:five_prime_UTR 10.0%
!!! TTTTATTTATATATGGCATA+AGG + tig0024351:25635-25654 None:intergenic 15.0%
!!! GTTATTTTGTTTCAAAAAAA+TGG + tig0024351:25757-25776 None:intergenic 15.0%
!!! TACTACTATTATCATTTTTT+TGG - tig0024351:25955-25974 Msa1416420:CDS 15.0%
!! TTATATAAAAATAGTTGAAC+TGG - tig0024351:26361-26380 Msa1416420:intron 15.0%
!! ATTATAAATTAAGTCTTCTT+AGG + tig0024351:27021-27040 None:intergenic 15.0%
!!! TTTGAAATAATAATTCTTGA+AGG - tig0024351:27103-27122 Msa1416420:five_prime_UTR 15.0%
!! TTGAAATAATAATTCTTGAA+GGG - tig0024351:27104-27123 Msa1416420:five_prime_UTR 15.0%
!!! TTTTATTTATATATGGCATA+AGG + tig0024351:25635-25654 None:intergenic 15.0%
!!! GTTATTTTGTTTCAAAAAAA+TGG + tig0024351:25757-25776 None:intergenic 15.0%
!!! TACTACTATTATCATTTTTT+TGG - tig0024351:25955-25974 Msa1416420:CDS 15.0%
!! TTATATAAAAATAGTTGAAC+TGG - tig0024351:26361-26380 Msa1416420:intron 15.0%
!! ATTATAAATTAAGTCTTCTT+AGG + tig0024351:27021-27040 None:intergenic 15.0%
!!! TTTGAAATAATAATTCTTGA+AGG - tig0024351:27103-27122 Msa1416420:five_prime_UTR 15.0%
!! TTGAAATAATAATTCTTGAA+GGG - tig0024351:27104-27123 Msa1416420:five_prime_UTR 15.0%
!! AAAAAAATTGTCCAATTTGT+TGG + tig0024351:25793-25812 None:intergenic 20.0%
!! CAAAGTTATGTCTAATATTA+TGG - tig0024351:26448-26467 Msa1416420:intron 20.0%
!! ATTTCTAAGTCCATAAAAAA+AGG - tig0024351:26778-26797 Msa1416420:intron 20.0%
!!! AAAGATTTCACCTTTTTTTA+TGG + tig0024351:26791-26810 None:intergenic 20.0%
!! TGTACTTGTAACATTTATTA+TGG - tig0024351:27140-27159 Msa1416420:five_prime_UTR 20.0%
!! AAAAAAATTGTCCAATTTGT+TGG + tig0024351:25793-25812 None:intergenic 20.0%
!! CAAAGTTATGTCTAATATTA+TGG - tig0024351:26448-26467 Msa1416420:intron 20.0%
!! ATTTCTAAGTCCATAAAAAA+AGG - tig0024351:26778-26797 Msa1416420:intron 20.0%
!!! AAAGATTTCACCTTTTTTTA+TGG + tig0024351:26791-26810 None:intergenic 20.0%
!! TGTACTTGTAACATTTATTA+TGG - tig0024351:27140-27159 Msa1416420:five_prime_UTR 20.0%
! ATACATATTAGTTAGTTCGT+TGG + tig0024351:25466-25485 None:intergenic 25.0%
!!! TTGCTTTGAATTTAAGTTGA+AGG - tig0024351:25549-25568 Msa1416420:three_prime_UTR 25.0%
! TGTATAATGCTTCATGTATA+TGG - tig0024351:25900-25919 Msa1416420:CDS 25.0%
! ATTTGAAGGAGTTAAAGAAT+CGG + tig0024351:26253-26272 None:intergenic 25.0%
!!! AAGTTGTTTTCATAATCACA+CGG + tig0024351:26343-26362 None:intergenic 25.0%
! GATTAAATTGTTTATGCAAC+TGG - tig0024351:26404-26423 Msa1416420:intron 25.0%
! TATTATGGTATGTCTTATGT+AGG - tig0024351:26463-26482 Msa1416420:intron 25.0%
! TGCATTATCAGAAGAATAAT+GGG - tig0024351:26830-26849 Msa1416420:intron 25.0%
!!! TTGCATTTTGTTACTTGATA+TGG - tig0024351:27165-27184 Msa1416420:five_prime_UTR 25.0%
! ATACATATTAGTTAGTTCGT+TGG + tig0024351:25466-25485 None:intergenic 25.0%
!!! TTGCTTTGAATTTAAGTTGA+AGG - tig0024351:25549-25568 Msa1416420:three_prime_UTR 25.0%
! TGTATAATGCTTCATGTATA+TGG - tig0024351:25900-25919 Msa1416420:CDS 25.0%
! ATTTGAAGGAGTTAAAGAAT+CGG + tig0024351:26253-26272 None:intergenic 25.0%
!!! AAGTTGTTTTCATAATCACA+CGG + tig0024351:26343-26362 None:intergenic 25.0%
! GATTAAATTGTTTATGCAAC+TGG - tig0024351:26404-26423 Msa1416420:intron 25.0%
! TATTATGGTATGTCTTATGT+AGG - tig0024351:26463-26482 Msa1416420:intron 25.0%
! TGCATTATCAGAAGAATAAT+GGG - tig0024351:26830-26849 Msa1416420:intron 25.0%
!!! TTGCATTTTGTTACTTGATA+TGG - tig0024351:27165-27184 Msa1416420:five_prime_UTR 25.0%
CTAATACTTACCATCACATT+TGG - tig0024351:25580-25599 Msa1416420:three_prime_UTR 30.0%
! AATGGTTGTTATTGATAGAG+TGG + tig0024351:25611-25630 None:intergenic 30.0%
TTATATATGGCATAAGGCAA+TGG + tig0024351:25629-25648 None:intergenic 30.0%
TGCGTTAGATCAATAATGAA+TGG + tig0024351:25720-25739 None:intergenic 30.0%
TAATCATGTCACCAACAAAT+TGG - tig0024351:25779-25798 Msa1416420:CDS 30.0%
CATTATACATTACCTGATGA+TGG + tig0024351:25889-25908 None:intergenic 30.0%
CTCTAACTTAGTGTTATGTT+TGG - tig0024351:25981-26000 Msa1416420:CDS 30.0%
! CAATGAGTTTTGCAAAATCA+AGG - tig0024351:26009-26028 Msa1416420:CDS 30.0%
TGTAGCTATTGCTAAAATTG+TGG - tig0024351:26062-26081 Msa1416420:CDS 30.0%
!! AGTTGTTTTCATAATCACAC+GGG + tig0024351:26342-26361 None:intergenic 30.0%
TATGTCTTATGTAGGTGTTA+AGG - tig0024351:26471-26490 Msa1416420:intron 30.0%
!!! TCCAGGAATTTTTTCAACAT+TGG + tig0024351:26611-26630 None:intergenic 30.0%
AGAATAGACTACAAACCTAA+TGG - tig0024351:26658-26677 Msa1416420:intron 30.0%
AAATCTTTGTGTTCCCAAAA+GGG - tig0024351:26803-26822 Msa1416420:intron 30.0%
GTGCATTATCAGAAGAATAA+TGG - tig0024351:26829-26848 Msa1416420:intron 30.0%
GCATTATCAGAAGAATAATG+GGG - tig0024351:26831-26850 Msa1416420:intron 30.0%
CTGTTTAGTATCAATCTGAA+AGG + tig0024351:26971-26990 None:intergenic 30.0%
CTTTCAGATTGATACTAAAC+AGG - tig0024351:26969-26988 Msa1416420:CDS 30.0%
! AGAATGTGTCACATTTTATG+TGG - tig0024351:27273-27292 Msa1416420:five_prime_UTR 30.0%
! GAATGTGTCACATTTTATGT+GGG - tig0024351:27274-27293 Msa1416420:five_prime_UTR 30.0%
! CACATTTTATGTGGGATATA+TGG - tig0024351:27282-27301 Msa1416420:five_prime_UTR 30.0%
CTAATACTTACCATCACATT+TGG - tig0024351:25580-25599 Msa1416420:three_prime_UTR 30.0%
! AATGGTTGTTATTGATAGAG+TGG + tig0024351:25611-25630 None:intergenic 30.0%
TTATATATGGCATAAGGCAA+TGG + tig0024351:25629-25648 None:intergenic 30.0%
TGCGTTAGATCAATAATGAA+TGG + tig0024351:25720-25739 None:intergenic 30.0%
TAATCATGTCACCAACAAAT+TGG - tig0024351:25779-25798 Msa1416420:CDS 30.0%
CATTATACATTACCTGATGA+TGG + tig0024351:25889-25908 None:intergenic 30.0%
CTCTAACTTAGTGTTATGTT+TGG - tig0024351:25981-26000 Msa1416420:CDS 30.0%
! CAATGAGTTTTGCAAAATCA+AGG - tig0024351:26009-26028 Msa1416420:CDS 30.0%
TGTAGCTATTGCTAAAATTG+TGG - tig0024351:26062-26081 Msa1416420:CDS 30.0%
!! AGTTGTTTTCATAATCACAC+GGG + tig0024351:26342-26361 None:intergenic 30.0%
TATGTCTTATGTAGGTGTTA+AGG - tig0024351:26471-26490 Msa1416420:intron 30.0%
!!! TCCAGGAATTTTTTCAACAT+TGG + tig0024351:26611-26630 None:intergenic 30.0%
AGAATAGACTACAAACCTAA+TGG - tig0024351:26658-26677 Msa1416420:intron 30.0%
AAATCTTTGTGTTCCCAAAA+GGG - tig0024351:26803-26822 Msa1416420:intron 30.0%
GTGCATTATCAGAAGAATAA+TGG - tig0024351:26829-26848 Msa1416420:intron 30.0%
GCATTATCAGAAGAATAATG+GGG - tig0024351:26831-26850 Msa1416420:intron 30.0%
CTGTTTAGTATCAATCTGAA+AGG + tig0024351:26971-26990 None:intergenic 30.0%
CTTTCAGATTGATACTAAAC+AGG - tig0024351:26969-26988 Msa1416420:CDS 30.0%
! AGAATGTGTCACATTTTATG+TGG - tig0024351:27273-27292 Msa1416420:five_prime_UTR 30.0%
! GAATGTGTCACATTTTATGT+GGG - tig0024351:27274-27293 Msa1416420:five_prime_UTR 30.0%
! CACATTTTATGTGGGATATA+TGG - tig0024351:27282-27301 Msa1416420:five_prime_UTR 30.0%
ATTGACAGTCCAATGAAAAG+AGG + tig0024351:25424-25443 None:intergenic 35.0%
! GTTGTTATTGATAGAGTGGA+TGG + tig0024351:25607-25626 None:intergenic 35.0%
AAGAGAGAAGAAAGTTGAGT+TGG + tig0024351:25682-25701 None:intergenic 35.0%
AGCTATTGCTAAAATTGTGG+AGG - tig0024351:26065-26084 Msa1416420:CDS 35.0%
ATTCCCTTATGTACTCTTGA+GGG - tig0024351:26142-26161 Msa1416420:CDS 35.0%
TATAGAGTGCATAGTTCACT+CGG + tig0024351:26289-26308 None:intergenic 35.0%
TATGTAGGTGTTAAGGTGAA+TGG - tig0024351:26478-26497 Msa1416420:intron 35.0%
!!! GCAGCTGATTTTTTCTTTGA+TGG - tig0024351:26532-26551 Msa1416420:intron 35.0%
GCCAATGTTGAAAAAATTCC+TGG - tig0024351:26607-26626 Msa1416420:intron 35.0%
TTGGAAGGTGAATTAGATGT+AGG - tig0024351:26730-26749 Msa1416420:intron 35.0%
GAAATCTTTGTGTTCCCAAA+AGG - tig0024351:26802-26821 Msa1416420:intron 35.0%
! GAAACAATTGACAAAGTACC+AGG + tig0024351:26898-26917 None:intergenic 35.0%
ATTGACAGTCCAATGAAAAG+AGG + tig0024351:25424-25443 None:intergenic 35.0%
! GTTGTTATTGATAGAGTGGA+TGG + tig0024351:25607-25626 None:intergenic 35.0%
AAGAGAGAAGAAAGTTGAGT+TGG + tig0024351:25682-25701 None:intergenic 35.0%
AGCTATTGCTAAAATTGTGG+AGG - tig0024351:26065-26084 Msa1416420:CDS 35.0%
ATTCCCTTATGTACTCTTGA+GGG - tig0024351:26142-26161 Msa1416420:CDS 35.0%
TATAGAGTGCATAGTTCACT+CGG + tig0024351:26289-26308 None:intergenic 35.0%
TATGTAGGTGTTAAGGTGAA+TGG - tig0024351:26478-26497 Msa1416420:intron 35.0%
!!! GCAGCTGATTTTTTCTTTGA+TGG - tig0024351:26532-26551 Msa1416420:intron 35.0%
GCCAATGTTGAAAAAATTCC+TGG - tig0024351:26607-26626 Msa1416420:intron 35.0%
TTGGAAGGTGAATTAGATGT+AGG - tig0024351:26730-26749 Msa1416420:intron 35.0%
GAAATCTTTGTGTTCCCAAA+AGG - tig0024351:26802-26821 Msa1416420:intron 35.0%
! GAAACAATTGACAAAGTACC+AGG + tig0024351:26898-26917 None:intergenic 35.0%
CAGTCCAATGAAAAGAGGTT+TGG + tig0024351:25419-25438 None:intergenic 40.0%
! TAGTTAGTTCGTTGGTTGCT+GGG + tig0024351:25458-25477 None:intergenic 40.0%
! TTAGTTAGTTCGTTGGTTGC+TGG + tig0024351:25459-25478 None:intergenic 40.0%
!! GAGAAGAAAGTTGAGTTGGA+TGG + tig0024351:25678-25697 None:intergenic 40.0%
CAAAGGTCTTGAAGTGTATC+AGG + tig0024351:25856-25875 None:intergenic 40.0%
! ATGATGGAAGAGCTACACAA+AGG + tig0024351:25873-25892 None:intergenic 40.0%
GATTCCCTTATGTACTCTTG+AGG - tig0024351:26141-26160 Msa1416420:CDS 40.0%
! AGACCCTCAAGAGTACATAA+GGG + tig0024351:26148-26167 None:intergenic 40.0%
GTTGAGCTAAGGGAATTTGA+AGG + tig0024351:26267-26286 None:intergenic 40.0%
TGATGGTTTAGCAAACCCTA+AGG - tig0024351:26549-26568 Msa1416420:intron 40.0%
! CAGTGTTGTTGACAACCTTA+GGG + tig0024351:26567-26586 None:intergenic 40.0%
! ACAGTGTTGTTGACAACCTT+AGG + tig0024351:26568-26587 None:intergenic 40.0%
AAGGTTGTCAACAACACTGT+AGG - tig0024351:26568-26587 Msa1416420:intron 40.0%
CAACAACACTGTAGGATCTT+TGG - tig0024351:26576-26595 Msa1416420:intron 40.0%
AATTCCTGGACTCAACACAT+TGG - tig0024351:26621-26640 Msa1416420:intron 40.0%
ATTCCTGGACTCAACACATT+GGG - tig0024351:26622-26641 Msa1416420:intron 40.0%
CAACACAAAAACAACCTCAG+TGG + tig0024351:26713-26732 None:intergenic 40.0%
!!! CACTGAGGTTGTTTTTGTGT+TGG - tig0024351:26711-26730 Msa1416420:intron 40.0%
!!! GAGGTTGTTTTTGTGTTGGA+AGG - tig0024351:26715-26734 Msa1416420:intron 40.0%
! GATAATGCACCAACCCTTTT+GGG + tig0024351:26819-26838 None:intergenic 40.0%
!! TGATAATGCACCAACCCTTT+TGG + tig0024351:26820-26839 None:intergenic 40.0%
! GCTTTTAACAGTCAACTTCC+TGG - tig0024351:26877-26896 Msa1416420:intron 40.0%
TCTGTTTGACTCTACACCAA+CGG - tig0024351:26921-26940 Msa1416420:CDS 40.0%
! GGTTTCTTTTCTTCTGTCTG+TGG - tig0024351:27303-27322 Msa1416420:five_prime_UTR 40.0%
!!! TGTGGTTATAGCTGCTTTTG+TGG - tig0024351:27321-27340 Msa1416420:five_prime_UTR 40.0%
!!! GTGGTTATAGCTGCTTTTGT+GGG - tig0024351:27322-27341 Msa1416420:five_prime_UTR 40.0%
TCCAATGAAAAGAGGTTTGG+TGG + tig0024351:25416-25435 None:intergenic 40.0%
CAGTCCAATGAAAAGAGGTT+TGG + tig0024351:25419-25438 None:intergenic 40.0%
! TAGTTAGTTCGTTGGTTGCT+GGG + tig0024351:25458-25477 None:intergenic 40.0%
! TTAGTTAGTTCGTTGGTTGC+TGG + tig0024351:25459-25478 None:intergenic 40.0%
!! GAGAAGAAAGTTGAGTTGGA+TGG + tig0024351:25678-25697 None:intergenic 40.0%
CAAAGGTCTTGAAGTGTATC+AGG + tig0024351:25856-25875 None:intergenic 40.0%
! ATGATGGAAGAGCTACACAA+AGG + tig0024351:25873-25892 None:intergenic 40.0%
GATTCCCTTATGTACTCTTG+AGG - tig0024351:26141-26160 Msa1416420:CDS 40.0%
! AGACCCTCAAGAGTACATAA+GGG + tig0024351:26148-26167 None:intergenic 40.0%
GTTGAGCTAAGGGAATTTGA+AGG + tig0024351:26267-26286 None:intergenic 40.0%
TGATGGTTTAGCAAACCCTA+AGG - tig0024351:26549-26568 Msa1416420:intron 40.0%
! CAGTGTTGTTGACAACCTTA+GGG + tig0024351:26567-26586 None:intergenic 40.0%
! ACAGTGTTGTTGACAACCTT+AGG + tig0024351:26568-26587 None:intergenic 40.0%
AAGGTTGTCAACAACACTGT+AGG - tig0024351:26568-26587 Msa1416420:intron 40.0%
CAACAACACTGTAGGATCTT+TGG - tig0024351:26576-26595 Msa1416420:intron 40.0%
AATTCCTGGACTCAACACAT+TGG - tig0024351:26621-26640 Msa1416420:intron 40.0%
ATTCCTGGACTCAACACATT+GGG - tig0024351:26622-26641 Msa1416420:intron 40.0%
CAACACAAAAACAACCTCAG+TGG + tig0024351:26713-26732 None:intergenic 40.0%
!!! CACTGAGGTTGTTTTTGTGT+TGG - tig0024351:26711-26730 Msa1416420:intron 40.0%
!!! GAGGTTGTTTTTGTGTTGGA+AGG - tig0024351:26715-26734 Msa1416420:intron 40.0%
! GATAATGCACCAACCCTTTT+GGG + tig0024351:26819-26838 None:intergenic 40.0%
!! TGATAATGCACCAACCCTTT+TGG + tig0024351:26820-26839 None:intergenic 40.0%
! GCTTTTAACAGTCAACTTCC+TGG - tig0024351:26877-26896 Msa1416420:intron 40.0%
TCTGTTTGACTCTACACCAA+CGG - tig0024351:26921-26940 Msa1416420:CDS 40.0%
! GGTTTCTTTTCTTCTGTCTG+TGG - tig0024351:27303-27322 Msa1416420:five_prime_UTR 40.0%
!!! TGTGGTTATAGCTGCTTTTG+TGG - tig0024351:27321-27340 Msa1416420:five_prime_UTR 40.0%
!!! GTGGTTATAGCTGCTTTTGT+GGG - tig0024351:27322-27341 Msa1416420:five_prime_UTR 40.0%
AGTGGATGGACCAAATGTGA+TGG + tig0024351:25593-25612 None:intergenic 45.0%
AGAAGCGAAGGTAGATGAGA+TGG + tig0024351:25830-25849 None:intergenic 45.0%
TGTATCAGGATCAGAAGCGA+AGG + tig0024351:25842-25861 None:intergenic 45.0%
TGTGTAGCTCTTCCATCATC+AGG - tig0024351:25874-25893 Msa1416420:CDS 45.0%
! CAGACCCTCAAGAGTACATA+AGG + tig0024351:26149-26168 None:intergenic 45.0%
TGTACTCTTGAGGGTCTGTT+TGG - tig0024351:26151-26170 Msa1416420:CDS 45.0%
TCTTGAGGGTCTGTTTGGAT+TGG - tig0024351:26156-26175 Msa1416420:CDS 45.0%
AGTTCACTCGGTTGAGCTAA+GGG + tig0024351:26277-26296 None:intergenic 45.0%
TAGTTCACTCGGTTGAGCTA+AGG + tig0024351:26278-26297 None:intergenic 45.0%
! GGTGAATGGATTTGCATGCA+AGG - tig0024351:26492-26511 Msa1416420:intron 45.0%
CTTTGTGTTCCCAAAAGGGT+TGG - tig0024351:26807-26826 Msa1416420:intron 45.0%
AGAACATCATCAGGAACCGT+TGG + tig0024351:26940-26959 None:intergenic 45.0%
! TCCTGATGATGTTCTTGCTC+AGG - tig0024351:26945-26964 Msa1416420:CDS 45.0%
!!! GCTTTTGTGGGCATTGAACA+AGG - tig0024351:27334-27353 Msa1416420:five_prime_UTR 45.0%
AGTGGATGGACCAAATGTGA+TGG + tig0024351:25593-25612 None:intergenic 45.0%
AGAAGCGAAGGTAGATGAGA+TGG + tig0024351:25830-25849 None:intergenic 45.0%
TGTATCAGGATCAGAAGCGA+AGG + tig0024351:25842-25861 None:intergenic 45.0%
TGTGTAGCTCTTCCATCATC+AGG - tig0024351:25874-25893 Msa1416420:CDS 45.0%
! CAGACCCTCAAGAGTACATA+AGG + tig0024351:26149-26168 None:intergenic 45.0%
TGTACTCTTGAGGGTCTGTT+TGG - tig0024351:26151-26170 Msa1416420:CDS 45.0%
TCTTGAGGGTCTGTTTGGAT+TGG - tig0024351:26156-26175 Msa1416420:CDS 45.0%
AGTTCACTCGGTTGAGCTAA+GGG + tig0024351:26277-26296 None:intergenic 45.0%
TAGTTCACTCGGTTGAGCTA+AGG + tig0024351:26278-26297 None:intergenic 45.0%
! GGTGAATGGATTTGCATGCA+AGG - tig0024351:26492-26511 Msa1416420:intron 45.0%
CTTTGTGTTCCCAAAAGGGT+TGG - tig0024351:26807-26826 Msa1416420:intron 45.0%
AGAACATCATCAGGAACCGT+TGG + tig0024351:26940-26959 None:intergenic 45.0%
! TCCTGATGATGTTCTTGCTC+AGG - tig0024351:26945-26964 Msa1416420:CDS 45.0%
!!! GCTTTTGTGGGCATTGAACA+AGG - tig0024351:27334-27353 Msa1416420:five_prime_UTR 45.0%
ACTCCCAATGTGTTGAGTCC+AGG + tig0024351:26628-26647 None:intergenic 50.0%
!! TGTGGCGGGTTAAGACCATT+AGG + tig0024351:26676-26695 None:intergenic 50.0%
AAACAACCTCAGTGGCACGA+GGG + tig0024351:26705-26724 None:intergenic 50.0%
AAAACAACCTCAGTGGCACG+AGG + tig0024351:26706-26725 None:intergenic 50.0%
GCCTGAGCAAGAACATCATC+AGG + tig0024351:26949-26968 None:intergenic 50.0%
ACTCCCAATGTGTTGAGTCC+AGG + tig0024351:26628-26647 None:intergenic 50.0%
!! TGTGGCGGGTTAAGACCATT+AGG + tig0024351:26676-26695 None:intergenic 50.0%
AAACAACCTCAGTGGCACGA+GGG + tig0024351:26705-26724 None:intergenic 50.0%
AAAACAACCTCAGTGGCACG+AGG + tig0024351:26706-26725 None:intergenic 50.0%
GCCTGAGCAAGAACATCATC+AGG + tig0024351:26949-26968 None:intergenic 50.0%
! CACGAGGGTGTGTGTGTGGC+GGG + tig0024351:26690-26709 None:intergenic 65.0%
! GCACGAGGGTGTGTGTGTGG+CGG + tig0024351:26691-26710 None:intergenic 65.0%
! GTGGCACGAGGGTGTGTGTG+TGG + tig0024351:26694-26713 None:intergenic 65.0%
! CACACACCCTCGTGCCACTG+AGG - tig0024351:26696-26715 Msa1416420:intron 65.0%
! CACGAGGGTGTGTGTGTGGC+GGG + tig0024351:26690-26709 None:intergenic 65.0%
! GCACGAGGGTGTGTGTGTGG+CGG + tig0024351:26691-26710 None:intergenic 65.0%
! GTGGCACGAGGGTGTGTGTG+TGG + tig0024351:26694-26713 None:intergenic 65.0%
! CACACACCCTCGTGCCACTG+AGG - tig0024351:26696-26715 Msa1416420:intron 65.0%
Chromosome Type Strat End Strand Name
tig0024351 gene 25415 27379 25415 ID=Msa1416420;Name=Msa1416420
tig0024351 mRNA 25415 27379 25415 ID=Msa1416420-mRNA-1;Parent=Msa1416420;Name=Msa1416420-mRNA-1;_AED=0.00;_eAED=0.00;_QI=369|1|1|1|1|1|2|347|219
tig0024351 exon 25415 26309 25415 ID=Msa1416420-mRNA-1:exon:22931;Parent=Msa1416420-mRNA-1
tig0024351 exon 26899 27379 26899 ID=Msa1416420-mRNA-1:exon:22930;Parent=Msa1416420-mRNA-1
tig0024351 five_prime_UTR 27011 27379 27011 ID=Msa1416420-mRNA-1:five_prime_utr;Parent=Msa1416420-mRNA-1
tig0024351 CDS 26899 27010 26899 ID=Msa1416420-mRNA-1:cds;Parent=Msa1416420-mRNA-1
tig0024351 CDS 25762 26309 25762 ID=Msa1416420-mRNA-1:cds;Parent=Msa1416420-mRNA-1
tig0024351 three_prime_UTR 25415 25761 25415 ID=Msa1416420-mRNA-1:three_prime_utr;Parent=Msa1416420-mRNA-1
Gene Sequence

>Msa1416420

ATGTCACCAACAAATTGGACAATTTTTTTCATTTTGTGCTGTGCCATCTCATCTACCTTCGCTTCTGATCCTGATACACTTCAAGACCTTTGTGTAGCTCTTCCATCATCAGGTGTTAAGGTGAATGGATTTGCATGCAAGGCAGAGTCAAATGTAACAGCAGCTGATTTTTTCTTTGATGGTTTAGCAAACCCTAAGGTTGTCAACAACACTGTAGGATCTTTGGTTACTGCAGCCAATGTTGAAAAAATTCCTGGACTCAACACATTGGGAGTATCTTTATCAAGAATAGACTACAAACCTAATGGTCTTAACCCGCCACACACACACCCTCGTGCCACTGAGGTTGTTTTTGTGTTGGAAGGTGAATTAGATGTAGGTTTTATCACTACATCAAACAAACTCATTTCTAAGTCCATAAAAAAAGGTGAAATCTTTGTGTTCCCAAAAGGGTTGGTGCATTATCAGAAGAATAATGGGGATAAAGCTGCTTCTGTGATTTCTGCTTTTAACAGTCAACTTCCTGGTACTTTGTCAATTGTTTCATCTCTGTTTGACTCTACACCAACGGTTCCTGATGATGTTCTTGCTCAGGCCTTTCAGATTGATACTAAACAGGTTGATGAAATAAAGACAAAGATTGCTCCTAAGAAGACTTAA
Protein sequence

>Msa1416420

MSPTNWTIFFILCCAISSTFASDPDTLQDLCVALPSSGVKVNGFACKAESNVTAADFFFDGLANPKVVNNTVGSLVTAANVEKIPGLNTLGVSLSRIDYKPNGLNPPHTHPRATEVVFVLEGELDVGFITTSNKLISKSIKKGEIFVFPKGLVHYQKNNGDKAASVISAFNSQLPGTLSIVSSLFDSTPTVPDDVLAQAFQIDTKQVDEIKTKIAPKKT*