AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1420640


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1420640 MtrunA17_Chr4g0006251 89.744 390 14 5 1 369 1 385 0.0 653
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTTAACTGATTTACTTCT+TGG 0.222003 tig0024833:-57529 None:intergenic
TTCTTTGGTGACACATTCTT+TGG 0.227592 tig0024833:-58036 None:intergenic
AGGTGTAATTGTAGTTATTA+TGG 0.236281 tig0024833:+57898 Msa1420640:CDS
TGTTCTGTCTGAATCTTCTT+TGG 0.263057 tig0024833:-58051 None:intergenic
GATGAAAATGGAACCCTTCT+TGG 0.264237 tig0024833:-57808 None:intergenic
CTTTGACAGAAGAAAGTATA+AGG 0.276142 tig0024833:+59866 Msa1420640:CDS
GTTAAGAAGGAGAAGAATAA+AGG 0.294047 tig0024833:+57751 Msa1420640:CDS
CAACTTCTTCATTGCCTACT+AGG 0.319844 tig0024833:+57956 Msa1420640:CDS
ACTTCTTGGAACAGAATCTA+AGG 0.322435 tig0024833:-57515 None:intergenic
TGTTCCTTTAACAACCCTTT+TGG 0.323978 tig0024833:-57491 None:intergenic
AGCACCAAAAGGGTTGTTAA+AGG 0.333890 tig0024833:+57487 Msa1420640:CDS
TGGTGTAGTAGCAAGGTTAA+TGG 0.335546 tig0024833:+57447 Msa1420640:CDS
AATTGTAGTTATTATGGATA+TGG 0.346675 tig0024833:+57904 Msa1420640:CDS
ATCAAAGACTGAAAGCTTAG+AGG 0.361130 tig0024833:+59903 Msa1420640:CDS
GGTGTAGTAGCAAGGTTAAT+GGG 0.365165 tig0024833:+57448 Msa1420640:CDS
TTAAGAAGGAGAAGAATAAA+GGG 0.377122 tig0024833:+57752 Msa1420640:CDS
AAACAAGAAAATTCAAGAAA+TGG 0.379420 tig0024833:+57292 Msa1420640:CDS
TACTTGGAAGAAGATGAAAA+TGG 0.380603 tig0024833:-57820 None:intergenic
GAGAAAGTAAACAATAATAA+AGG 0.383541 tig0024833:+59775 Msa1420640:CDS
AAGCTTAGAGGAAATTTGTT+TGG 0.383976 tig0024833:+59915 Msa1420640:CDS
ATCTTTATTGCCAGTGTCCT+TGG 0.392267 tig0024833:+59738 Msa1420640:intron
TTCTCTGTTGATGTCAGTGT+GGG 0.417009 tig0024833:-59806 None:intergenic
GGAAATTGTGAGAGTTAAGA+AGG 0.438622 tig0024833:+57738 Msa1420640:CDS
CTTCTTCCAAGTATAAATCA+AGG 0.448989 tig0024833:+57830 Msa1420640:CDS
TTGACATCTAGAACTGAAAC+AGG 0.458423 tig0024833:-58093 None:intergenic
CTCTTCAACATCTTCTTCCA+AGG 0.463668 tig0024833:-59755 None:intergenic
GATGATTCTATTACTACTCC+TGG 0.467242 tig0024833:+57427 Msa1420640:CDS
TCTAACCCTTGATTTATACT+TGG 0.472162 tig0024833:-57836 None:intergenic
TATTATGGATATGGTAATGC+TGG 0.473019 tig0024833:+57913 Msa1420640:CDS
TTGCCTACTAGGCAAAAGAA+AGG 0.483545 tig0024833:+57967 Msa1420640:CDS
ATTCACTTCCAAGCACCAAA+AGG 0.486866 tig0024833:+57476 Msa1420640:CDS
TTCACTTCCAAGCACCAAAA+GGG 0.495119 tig0024833:+57477 Msa1420640:CDS
GCTTGAGTTTGATCAAAACA+TGG 0.523774 tig0024833:+57564 Msa1420640:CDS
TTTGACAGAAGAAAGTATAA+GGG 0.526153 tig0024833:+59867 Msa1420640:CDS
TGCCTACTAGGCAAAAGAAA+GGG 0.548532 tig0024833:+57968 Msa1420640:CDS
AGTTAAAAGATGAACCAAGA+AGG 0.554790 tig0024833:+57794 Msa1420640:CDS
CTTGAGTTTGATCAAAACAT+GGG 0.565956 tig0024833:+57565 Msa1420640:CDS
CTTCTCTGTTGATGTCAGTG+TGG 0.574065 tig0024833:-59807 None:intergenic
GTTAAAAGATGAACCAAGAA+GGG 0.583184 tig0024833:+57795 Msa1420640:CDS
GATTCAGACAGAACATAGCA+TGG 0.587606 tig0024833:+58059 Msa1420640:CDS
TTCTTCCAAGTATAAATCAA+GGG 0.598400 tig0024833:+57831 Msa1420640:CDS
CTACTCCTGGTGTAGTAGCA+AGG 0.604802 tig0024833:+57440 Msa1420640:CDS
AAACTTGAGAATAAAGAGCA+AGG 0.615672 tig0024833:+57717 Msa1420640:CDS
ATTAACCTTGCTACTACACC+AGG 0.623143 tig0024833:-57445 None:intergenic
GTGTAGTAGCAAGGTTAATG+GGG 0.625800 tig0024833:+57449 Msa1420640:CDS
TCTCTGTTGATGTCAGTGTG+GGG 0.670330 tig0024833:-59805 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTGATTTAATATTTTTAA+TGG + tig0024833:58324-58343 Msa1420640:intron 10.0%
!! AAATATTAAATGATCAAAAA+TGG - tig0024833:59953-59972 None:intergenic 10.0%
!! AAAATAAAAATTAGCAACAA+GGG - tig0024833:58169-58188 None:intergenic 15.0%
!! AAAAATAAAAATTAGCAACA+AGG - tig0024833:58170-58189 None:intergenic 15.0%
!!! AATTCATTCCAAAATATTTT+AGG - tig0024833:58274-58293 None:intergenic 15.0%
!!! TTTTTTTTAAAAACATTGAG+AGG - tig0024833:58531-58550 None:intergenic 15.0%
!!! TAAAAATAATTTTTAGAGTG+AGG + tig0024833:58653-58672 Msa1420640:intron 15.0%
!!! TTAACTTTAAAAGAAAAAAG+AGG + tig0024833:58795-58814 Msa1420640:intron 15.0%
!!! AATATTAGATTTTTTAGGTA+GGG - tig0024833:59302-59321 None:intergenic 15.0%
!!! AAATATTAGATTTTTTAGGT+AGG - tig0024833:59303-59322 None:intergenic 15.0%
!!! CTACAAATATTAGATTTTTT+AGG - tig0024833:59307-59326 None:intergenic 15.0%
!! AAACAAGAAAATTCAAGAAA+TGG + tig0024833:57292-57311 Msa1420640:CDS 20.0%
!!! CTTGATATTGATGATAAAAA+AGG + tig0024833:57670-57689 Msa1420640:CDS 20.0%
!!! TATTGATGATAAAAAAGGTA+AGG + tig0024833:57675-57694 Msa1420640:CDS 20.0%
!! AATTGTAGTTATTATGGATA+TGG + tig0024833:57904-57923 Msa1420640:CDS 20.0%
!! TTGTGAATTACTTATTTACA+AGG + tig0024833:58210-58229 Msa1420640:intron 20.0%
!!! ATTCCAAAATATTTTAGGTT+CGG - tig0024833:58269-58288 None:intergenic 20.0%
!!! TAAATGCATTTTTAATCAAC+AGG - tig0024833:59082-59101 None:intergenic 20.0%
!! TAACTGTAATCAAATATTGA+GGG - tig0024833:59151-59170 None:intergenic 20.0%
!! TTCACAATTTAAACACATAA+AGG - tig0024833:59185-59204 None:intergenic 20.0%
!! GTAAAAAAGTGAACATATTA+TGG + tig0024833:59559-59578 Msa1420640:intron 20.0%
!! GAGAAAGTAAACAATAATAA+AGG + tig0024833:59775-59794 Msa1420640:CDS 20.0%
!! ACATTTTCAGATGGATATAT+TGG - tig0024833:57373-57392 None:intergenic 25.0%
!! ATTGTTTCAACATTTTCAGA+TGG - tig0024833:57382-57401 None:intergenic 25.0%
!! AAGTTAACTGATTTACTTCT+TGG - tig0024833:57532-57551 None:intergenic 25.0%
! TTAAGAAGGAGAAGAATAAA+GGG + tig0024833:57752-57771 Msa1420640:CDS 25.0%
! TTCTTCCAAGTATAAATCAA+GGG + tig0024833:57831-57850 Msa1420640:CDS 25.0%
!!! ATTTTTCAAGTGTTTCTACA+AGG + tig0024833:57878-57897 Msa1420640:CDS 25.0%
! AGGTGTAATTGTAGTTATTA+TGG + tig0024833:57898-57917 Msa1420640:CDS 25.0%
!! AATGATTATGCTTTTCTCTA+TGG + tig0024833:58114-58133 Msa1420640:CDS 25.0%
! AAGTAATTCACAAACTAGAA+TGG - tig0024833:58203-58222 None:intergenic 25.0%
! GAATTACTTATTTACAAGGT+TGG + tig0024833:58214-58233 Msa1420640:intron 25.0%
!! TTTCCGAACCTAAAATATTT+TGG + tig0024833:58263-58282 Msa1420640:intron 25.0%
!! TTCTAACAATTTAGAGTCAT+TGG + tig0024833:58379-58398 Msa1420640:intron 25.0%
!! TCTAACAATTTAGAGTCATT+GGG + tig0024833:58380-58399 Msa1420640:intron 25.0%
!! ATGAATTGTGACTTTTGTAT+TGG + tig0024833:58499-58518 Msa1420640:intron 25.0%
! GTTGCGTATATAGTAAATTA+AGG - tig0024833:58621-58640 None:intergenic 25.0%
!! TTTAGAGTGAGGAAATATTT+AGG + tig0024833:58664-58683 Msa1420640:intron 25.0%
! TTAGAGTGAGGAAATATTTA+GGG + tig0024833:58665-58684 Msa1420640:intron 25.0%
! TTTAAAAATATGAAGGACCT+TGG - tig0024833:58745-58764 None:intergenic 25.0%
! AAAGAGGAAACAAACATATA+CGG + tig0024833:58811-58830 Msa1420640:intron 25.0%
! GTAACTGTAATCAAATATTG+AGG - tig0024833:59152-59171 None:intergenic 25.0%
! AATTTAAACACATAAAGGCA+AGG - tig0024833:59180-59199 None:intergenic 25.0%
! TTGTGACAATATTCTTTGAA+AGG + tig0024833:59397-59416 Msa1420640:intron 25.0%
!!! CGTAGTCTTTTTTTTATTAC+AGG - tig0024833:59456-59475 None:intergenic 25.0%
!!! GGCAATTTTGTTTAATTCTT+TGG + tig0024833:59489-59508 Msa1420640:intron 25.0%
!!! CTTTTTGACTTTAACTTTGT+TGG - tig0024833:59709-59728 None:intergenic 25.0%
! CAACAAAGTTAAAGTCAAAA+AGG + tig0024833:59707-59726 Msa1420640:intron 25.0%
!! TTTGACAGAAGAAAGTATAA+GGG + tig0024833:59867-59886 Msa1420640:CDS 25.0%
! TTTTCAGATGGATATATTGG+TGG - tig0024833:57370-57389 None:intergenic 30.0%
CTTGAGTTTGATCAAAACAT+GGG + tig0024833:57565-57584 Msa1420640:CDS 30.0%
!! TTTTAGAGAAGTTCCTTCAA+TGG + tig0024833:57618-57637 Msa1420640:CDS 30.0%
! TTTTGCTTATCAACCATTGA+AGG - tig0024833:57634-57653 None:intergenic 30.0%
AAACTTGAGAATAAAGAGCA+AGG + tig0024833:57717-57736 Msa1420640:CDS 30.0%
GTTAAGAAGGAGAAGAATAA+AGG + tig0024833:57751-57770 Msa1420640:CDS 30.0%
AGTTAAAAGATGAACCAAGA+AGG + tig0024833:57794-57813 Msa1420640:CDS 30.0%
GTTAAAAGATGAACCAAGAA+GGG + tig0024833:57795-57814 Msa1420640:CDS 30.0%
TACTTGGAAGAAGATGAAAA+TGG - tig0024833:57823-57842 None:intergenic 30.0%
CTTCTTCCAAGTATAAATCA+AGG + tig0024833:57830-57849 Msa1420640:CDS 30.0%
TCTAACCCTTGATTTATACT+TGG - tig0024833:57839-57858 None:intergenic 30.0%
! TATTATGGATATGGTAATGC+TGG + tig0024833:57913-57932 Msa1420640:CDS 30.0%
! TTAGCATCTAGAACTTTCAA+TGG + tig0024833:58297-58316 Msa1420640:intron 30.0%
TAGAGTGAGGAAATATTTAG+GGG + tig0024833:58666-58685 Msa1420640:intron 30.0%
ACAAACACGTAGTAATCAAA+TGG - tig0024833:59381-59400 None:intergenic 30.0%
TTAACTTTGTTGGAGAGAAT+AGG - tig0024833:59699-59718 None:intergenic 30.0%
! CTTTGACAGAAGAAAGTATA+AGG + tig0024833:59866-59885 Msa1420640:CDS 30.0%
! AAGCTTAGAGGAAATTTGTT+TGG + tig0024833:59915-59934 Msa1420640:CDS 30.0%
GATGATTCTATTACTACTCC+TGG + tig0024833:57427-57446 Msa1420640:CDS 35.0%
! TGTTCCTTTAACAACCCTTT+TGG - tig0024833:57494-57513 None:intergenic 35.0%
ACTTCTTGGAACAGAATCTA+AGG - tig0024833:57518-57537 None:intergenic 35.0%
GCTTGAGTTTGATCAAAACA+TGG + tig0024833:57564-57583 Msa1420640:CDS 35.0%
GGAAATTGTGAGAGTTAAGA+AGG + tig0024833:57738-57757 Msa1420640:CDS 35.0%
!! GGTTTGTTGAACCAAAAATG+AGG + tig0024833:57989-58008 Msa1420640:CDS 35.0%
TGAACCAAAAATGAGGAACA+AGG + tig0024833:57996-58015 Msa1420640:CDS 35.0%
! TTCACCTTGTTCCTCATTTT+TGG - tig0024833:58003-58022 None:intergenic 35.0%
! TTCTTTGGTGACACATTCTT+TGG - tig0024833:58039-58058 None:intergenic 35.0%
TGTTCTGTCTGAATCTTCTT+TGG - tig0024833:58054-58073 None:intergenic 35.0%
! TTGACATCTAGAACTGAAAC+AGG - tig0024833:58096-58115 None:intergenic 35.0%
! TCCTTACACAACTTGTTTAC+TGG + tig0024833:58418-58437 Msa1420640:intron 35.0%
ACCAGTAAACAAGTTGTGTA+AGG - tig0024833:58422-58441 None:intergenic 35.0%
! TTACACAACTTGTTTACTGG+TGG + tig0024833:58421-58440 Msa1420640:intron 35.0%
AGAGTGAGGAAATATTTAGG+GGG + tig0024833:58667-58686 Msa1420640:intron 35.0%
CAATGTTGCTCTTTGTAAGA+AGG - tig0024833:58937-58956 None:intergenic 35.0%
AGACTACGAGTATTATTCTG+TGG + tig0024833:59468-59487 Msa1420640:intron 35.0%
ATCAAAGACTGAAAGCTTAG+AGG + tig0024833:59903-59922 Msa1420640:CDS 35.0%
ATTAACCTTGCTACTACACC+AGG - tig0024833:57448-57467 None:intergenic 40.0%
TGGTGTAGTAGCAAGGTTAA+TGG + tig0024833:57447-57466 Msa1420640:CDS 40.0%
GGTGTAGTAGCAAGGTTAAT+GGG + tig0024833:57448-57467 Msa1420640:CDS 40.0%
GTGTAGTAGCAAGGTTAATG+GGG + tig0024833:57449-57468 Msa1420640:CDS 40.0%
ATTCACTTCCAAGCACCAAA+AGG + tig0024833:57476-57495 Msa1420640:CDS 40.0%
TTCACTTCCAAGCACCAAAA+GGG + tig0024833:57477-57496 Msa1420640:CDS 40.0%
!!! TTAACAACCCTTTTGGTGCT+TGG - tig0024833:57487-57506 None:intergenic 40.0%
AGCACCAAAAGGGTTGTTAA+AGG + tig0024833:57487-57506 Msa1420640:CDS 40.0%
! ATGCTGAAGTTTTCACACGT+CGG - tig0024833:57597-57616 None:intergenic 40.0%
GATGAAAATGGAACCCTTCT+TGG - tig0024833:57811-57830 None:intergenic 40.0%
CAACTTCTTCATTGCCTACT+AGG + tig0024833:57956-57975 Msa1420640:CDS 40.0%
TTGCCTACTAGGCAAAAGAA+AGG + tig0024833:57967-57986 Msa1420640:CDS 40.0%
TGCCTACTAGGCAAAAGAAA+GGG + tig0024833:57968-57987 Msa1420640:CDS 40.0%
! AACCCTTTCTTTTGCCTAGT+AGG - tig0024833:57973-57992 None:intergenic 40.0%
GATTCAGACAGAACATAGCA+TGG + tig0024833:58059-58078 Msa1420640:CDS 40.0%
!! CTCTATGGAGCTGATTTTTC+AGG + tig0024833:58129-58148 Msa1420640:CDS 40.0%
ATCTTTATTGCCAGTGTCCT+TGG + tig0024833:59738-59757 Msa1420640:intron 40.0%
CTCTTCAACATCTTCTTCCA+AGG - tig0024833:59758-59777 None:intergenic 40.0%
TTCTCTGTTGATGTCAGTGT+GGG - tig0024833:59809-59828 None:intergenic 40.0%
GTGAGGAAATATTTAGGGGG+CGG + tig0024833:58670-58689 Msa1420640:intron 45.0%
AAGGACCTTGGACTTCTACT+TGG - tig0024833:58733-58752 None:intergenic 45.0%
ACATCTTCTTCCAAGGACAC+TGG - tig0024833:59751-59770 None:intergenic 45.0%
TCTCTGTTGATGTCAGTGTG+GGG - tig0024833:59808-59827 None:intergenic 45.0%
CTTCTCTGTTGATGTCAGTG+TGG - tig0024833:59810-59829 None:intergenic 45.0%
!!! TTTTAAATTTAAAAATATGA+AGG - tig0024833:58752-58771 None:intergenic 5.0%
CTACTCCTGGTGTAGTAGCA+AGG + tig0024833:57440-57459 Msa1420640:CDS 50.0%
CGTCACCAAGTAGAAGTCCA+AGG + tig0024833:58725-58744 Msa1420640:intron 50.0%
GACAGTGACTCGTGCGATGT+CGG - tig0024833:58867-58886 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0024833 gene 57286 59996 57286 ID=Msa1420640;Name=Msa1420640
tig0024833 mRNA 57286 59996 57286 ID=Msa1420640-mRNA-1;Parent=Msa1420640;Name=Msa1420640-mRNA-1;_AED=0.04;_eAED=0.05;_QI=0|0|0|1|1|1|2|0|369
tig0024833 exon 57286 58150 57286 ID=Msa1420640-mRNA-1:exon:1603;Parent=Msa1420640-mRNA-1
tig0024833 exon 59752 59996 59752 ID=Msa1420640-mRNA-1:exon:1604;Parent=Msa1420640-mRNA-1
tig0024833 CDS 57286 58150 57286 ID=Msa1420640-mRNA-1:cds;Parent=Msa1420640-mRNA-1
tig0024833 CDS 59752 59996 59752 ID=Msa1420640-mRNA-1:cds;Parent=Msa1420640-mRNA-1
Gene Sequence

>Msa1420640

ATGGAAAAACAAGAAAATTCAAGAAATGGATGTTTCTCTAGTTTCATAAAAGTGTTACTTTGTGCTAGAAATGAAACTAGTCCACCAATATATCCATCTGAAAATGTTGAAACAATTCATCACAAAAAAGATAAACTTTTTGATGATTCTATTACTACTCCTGGTGTAGTAGCAAGGTTAATGGGGTTAGATTCACTTCCAAGCACCAAAAGGGTTGTTAAAGGAACAACCTTAGATTCTGTTCCAAGAAGTAAATCAGTTAACTTTGTTGATTACTTGCTTGAGTTTGATCAAAACATGGGAAGTCACCGACGTGTGAAAACTTCAGCATCTTTTAGAGAAGTTCCTTCAATGGTTGATAAGCAAAAAAGTTTCGTTTTTGTTCTTGATATTGATGATAAAAAAGGTAAGGTACAAGAAGAAAATGATGCAAACTTGAGAATAAAGAGCAAGGAAATTGTGAGAGTTAAGAAGGAGAAGAATAAAGGGAAGAACAAGAAAATTTCTAAGTTAAAAGATGAACCAAGAAGGGTTCCATTTTCATCTTCTTCCAAGTATAAATCAAGGGTTAGAGATTGTAGTAAAGATAAAGATTTTTCAAGTGTTTCTACAAGGTGTAATTGTAGTTATTATGGATATGGTAATGCTGGTTCAAGTTCAAGTTCATGTTCAACTTCTTCATTGCCTACTAGGCAAAAGAAAGGGTTTGTTGAACCAAAAATGAGGAACAAGGTGAAAAAACATGTGTCACCAAAGAATGTGTCACCAAAGAAGATTCAGACAGAACATAGCATGGAAAATCTTAGTCCTGTTTCAGTTCTAGATGTCAATGATTATGCTTTTCTCTATGGAGCTGATTTTTCAGTGTCCTTGGAAGAAGATGTTGAAGAGAAAGTAAACAATAATAAAGGATATGCTCCCCACACTGACATCAACAGAGAAGCAGAGTATTACTCAGACTTGATGTTGAAGCTTCGTTCTTTGACAGAAGAAAGTATAAGGGAGTCAGATTGCACATCAAAGACTGAAAGCTTAGAGGAAATTTGTTTGGTTTTTGAACAAACCATTTTTGATCATTTAATATTTGAGTTCTTAAATGAAGTTGTATAA

Protein sequence

>Msa1420640

MEKQENSRNGCFSSFIKVLLCARNETSPPIYPSENVETIHHKKDKLFDDSITTPGVVARLMGLDSLPSTKRVVKGTTLDSVPRSKSVNFVDYLLEFDQNMGSHRRVKTSASFREVPSMVDKQKSFVFVLDIDDKKGKVQEENDANLRIKSKEIVRVKKEKNKGKNKKISKLKDEPRRVPFSSSSKYKSRVRDCSKDKDFSSVSTRCNCSYYGYGNAGSSSSSCSTSSLPTRQKKGFVEPKMRNKVKKHVSPKNVSPKKIQTEHSMENLSPVSVLDVNDYAFLYGADFSVSLEEDVEEKVNNNKGYAPHTDINREAEYYSDLMLKLRSLTEESIRESDCTSKTESLEEICLVFEQTIFDHLIFEFLNEVV*