AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0084330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0084330 MtrunA17_Chr1g0199991 97.168 459 12 1 1 458 1 459 0.0 927
Msa0084330 MtrunA17_Chr1g0199971 84.279 458 71 1 1 457 1 458 0.0 812
Msa0084330 MtrunA17_Chr2g0309521 54.248 459 204 4 3 456 6 463 0.0 537
Msa0084330 MtrunA17_Chr2g0309541 54.505 455 197 5 8 456 14 464 0.0 527
Msa0084330 MtrunA17_Chr2g0309511 53.680 462 202 6 3 456 6 463 0.0 524
Msa0084330 MtrunA17_Chr7g0219051 54.881 461 192 8 7 456 10 465 0.0 517
Msa0084330 MtrunA17_Chr2g0318911 53.540 452 206 4 8 456 14 464 0.0 516
Msa0084330 MtrunA17_Chr3g0090331 49.302 430 207 6 28 455 70 490 1.03e-154 448
Msa0084330 MtrunA17_Chr3g0118841 51.765 425 192 7 26 449 72 484 6.11e-153 443
Msa0084330 MtrunA17_Chr4g0038311 48.498 466 224 10 1 455 3 463 1.13e-151 439
Msa0084330 MtrunA17_Chr3g0118881 49.886 437 197 8 31 457 76 500 2.08e-150 437
Msa0084330 MtrunA17_Chr3g0118851 50.588 425 198 7 26 449 71 484 3.42e-147 429
Msa0084330 MtrunA17_Chr4g0020241 48.956 431 207 8 28 456 69 488 8.74e-146 425
Msa0084330 MtrunA17_Chr1g0153881 49.199 437 199 8 19 449 72 491 3.22e-145 424
Msa0084330 MtrunA17_Chr4g0047861 46.847 444 219 7 27 457 48 487 8.44e-140 410
Msa0084330 MtrunA17_Chr4g0068841 44.328 476 240 8 5 458 8 480 9.51e-140 409
Msa0084330 MtrunA17_Chr3g0118831 48.492 431 177 8 29 457 74 461 1.68e-136 400
Msa0084330 MtrunA17_Chr2g0281621 44.444 468 242 9 3 457 5 467 2.35e-135 397
Msa0084330 MtrunA17_Chr1g0153941 45.077 457 215 7 17 449 62 506 3.38e-134 396
Msa0084330 MtrunA17_Chr3g0118861 48.157 434 171 8 28 449 71 462 2.32e-132 390
Msa0084330 MtrunA17_Chr3g0118891 47.294 425 179 8 26 449 71 451 4.18e-131 387
Msa0084330 MtrunA17_Chr4g0068861 44.393 428 211 8 8 411 52 476 3.21e-121 362
Msa0084330 MtrunA17_Chr2g0281641 45.312 384 194 5 87 456 7 388 2.77e-116 346
Msa0084330 MtrunA17_Chr8g0385871 39.871 464 253 12 12 457 9 464 1.08e-114 345
Msa0084330 MtrunA17_Chr3g0126031 42.333 463 248 11 6 458 6 459 1.53e-113 342
Msa0084330 MtrunA17_Chr2g0304931 42.212 443 231 10 28 457 34 464 1.92e-113 342
Msa0084330 MtrunA17_Chr5g0431301 41.164 464 252 10 8 458 9 464 2.50e-112 339
Msa0084330 MtrunA17_Chr3g0131731 38.266 473 260 13 1 455 3 461 2.75e-111 336
Msa0084330 MtrunA17_Chr3g0094811 41.075 465 241 13 5 456 11 455 8.58e-110 332
Msa0084330 MtrunA17_Chr7g0221551 40.547 439 237 12 31 455 29 457 5.12e-108 327
Msa0084330 MtrunA17_Chr3g0126051 40.319 439 249 9 30 458 31 466 2.93e-104 318
Msa0084330 MtrunA17_Chr8g0338461 35.197 483 252 13 19 449 17 490 1.24e-80 258
Msa0084330 MtrunA17_Chr1g0153931 56.000 200 84 3 17 214 60 257 9.55e-75 235
Msa0084330 MtrunA17_Chr7g0249431 34.517 507 255 19 5 454 12 498 1.30e-73 239
Msa0084330 MtrunA17_Chr7g0234051 32.929 495 273 14 5 449 5 490 1.10e-71 234
Msa0084330 MtrunA17_Chr1g0204191 33.556 450 235 14 26 455 85 490 2.75e-71 233
Msa0084330 MtrunA17_Chr1g0165811 46.847 222 108 4 29 250 27 238 1.84e-68 219
Msa0084330 MtrunA17_Chr7g0234041 31.291 457 250 15 6 414 4 444 9.33e-56 192
Msa0084330 MtrunA17_Chr1g0153921 45.495 222 107 6 229 449 2 210 1.84e-54 181
Msa0084330 MtrunA17_Chr2g0285351 30.417 480 288 18 2 454 9 469 9.22e-53 184
Msa0084330 MtrunA17_Chr4g0009701 31.222 442 269 14 32 451 39 467 3.98e-52 182
Msa0084330 MtrunA17_Chr2g0285371 29.828 466 282 17 15 454 23 469 4.02e-52 182
Msa0084330 MtrunA17_Chr4g0009661 31.236 445 265 15 32 451 39 467 4.90e-52 182
Msa0084330 MtrunA17_Chr4g0009681 30.786 471 289 17 4 451 8 464 2.60e-51 180
Msa0084330 MtrunA17_Chr3g0133011 30.480 479 292 15 8 454 27 496 5.37e-51 180
Msa0084330 MtrunA17_Chr4g0009651 30.337 445 269 15 32 451 39 467 7.08e-51 179
Msa0084330 MtrunA17_Chr4g0009711 28.970 466 293 15 8 451 18 467 2.06e-48 172
Msa0084330 MtrunA17_Chr3g0093971 28.017 464 297 13 1 446 2 446 2.91e-48 171
Msa0084330 MtrunA17_Chr4g0009641 29.195 435 263 12 32 451 39 443 2.89e-47 169
Msa0084330 MtrunA17_Chr4g0009671 29.655 435 259 14 45 451 17 432 7.42e-47 167
Msa0084330 MtrunA17_Chr2g0285381 28.633 461 291 15 13 454 24 465 9.16e-46 165
Msa0084330 MtrunA17_Chr4g0028271 28.738 428 249 14 47 450 97 492 3.05e-41 153
Msa0084330 MtrunA17_Chr2g0327401 27.635 427 260 14 47 452 100 498 3.30e-41 153
Msa0084330 MtrunA17_Chr4g0044051 29.314 423 254 16 49 454 47 441 1.12e-40 151
Msa0084330 MtrunA17_Chr1g0171411 28.169 426 251 15 45 446 81 475 2.51e-40 150
Msa0084330 MtrunA17_Chr4g0028261 28.306 431 264 13 38 449 109 513 6.88e-40 150
Msa0084330 MtrunA17_Chr4g0028281 27.700 426 260 15 47 452 95 492 2.34e-38 145
Msa0084330 MtrunA17_Chr4g0028241 26.906 446 272 13 32 452 74 490 4.37e-38 144
Msa0084330 MtrunA17_Chr1g0199171 27.208 419 255 14 49 446 92 481 8.12e-38 144
Msa0084330 MtrunA17_Chr2g0327381 36.937 222 132 5 47 266 94 309 5.22e-36 139
Msa0084330 MtrunA17_Chr5g0447441 27.316 421 265 14 49 454 43 437 1.89e-35 136
Msa0084330 MtrunA17_Chr2g0327371 26.709 468 238 17 50 452 86 513 5.78e-34 133
Msa0084330 MtrunA17_Chr4g0009721 26.752 471 261 18 4 451 7 416 1.83e-32 127
Msa0084330 MtrunA17_Chr1g0165821 37.908 153 87 3 308 455 29 178 3.17e-28 110
Msa0084330 MtrunA17_Chr7g0229631 45.528 123 51 6 8 124 7 119 3.92e-25 100
Msa0084330 MtrunA17_Chr7g0229611 46.078 102 41 5 10 105 1 94 3.77e-20 85.1
Msa0084330 MtrunA17_Chr7g0229621 48.515 101 43 5 5 104 1 93 7.11e-20 84.7
Msa0084330 MtrunA17_Chr7g0229651 27.551 196 133 4 214 401 1 195 8.95e-20 87.4
Msa0084330 MtrunA17_Chr2g0279371 32.105 190 104 7 19 202 14 184 9.76e-18 81.3
Msa0084330 MtrunA17_Chr4g0027731 30.128 156 96 5 41 192 27 173 2.79e-14 74.7
Msa0084330 MtrunA17_Chr2g0281631 44.792 96 35 5 3 87 9 97 6.13e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0084330 AT3G07990.1 75.862 435 104 1 22 455 23 457 0.0 708
Msa0084330 AT2G35780.1 70.252 437 123 4 22 455 19 451 0.0 648
Msa0084330 AT4G30610.1 55.556 468 193 7 1 457 1 464 0.0 538
Msa0084330 AT4G30810.1 58.005 431 175 3 23 449 28 456 0.0 533
Msa0084330 AT2G24010.1 55.632 435 187 5 25 457 23 453 1.26e-179 510
Msa0084330 AT2G24000.1 55.481 447 185 7 22 457 20 463 2.79e-179 509
Msa0084330 AT3G02110.1 55.134 448 180 8 26 457 31 473 3.20e-178 507
Msa0084330 AT2G24010.2 53.556 450 188 6 25 457 23 468 1.92e-174 498
Msa0084330 AT2G35770.1 54.031 459 201 6 3 457 8 460 3.03e-173 494
Msa0084330 AT2G24010.3 51.178 467 182 7 33 457 2 464 1.91e-166 477
Msa0084330 AT5G23210.1 50.110 455 196 9 28 456 47 496 8.79e-151 438
Msa0084330 AT3G63470.1 48.858 438 203 10 28 458 77 500 2.70e-149 435
Msa0084330 AT3G17180.1 48.889 450 210 9 25 457 31 477 5.47e-144 420
Msa0084330 AT1G61130.1 46.996 466 233 9 1 457 2 462 6.23e-142 414
Msa0084330 AT5G08260.1 43.816 477 236 11 5 456 8 477 4.30e-136 400
Msa0084330 AT1G11080.2 46.799 453 219 7 24 457 42 491 2.22e-133 394
Msa0084330 AT1G11080.1 46.799 453 219 7 24 457 42 491 2.40e-133 394
Msa0084330 AT2G24010.4 53.203 359 147 6 33 374 2 356 3.97e-132 385
Msa0084330 AT3G52020.1 46.560 436 217 12 26 455 73 498 1.88e-131 389
Msa0084330 AT5G23210.3 49.268 410 177 9 28 411 47 451 5.83e-130 384
Msa0084330 AT4G15100.1 43.633 479 243 9 3 457 8 483 1.28e-127 379
Msa0084330 AT5G23210.5 48.139 403 181 7 80 456 1 401 7.86e-125 369
Msa0084330 AT3G17180.2 46.699 409 199 8 25 417 31 436 1.10e-123 367
Msa0084330 AT5G23210.2 48.354 395 176 7 88 456 8 400 1.56e-123 365
Msa0084330 AT2G05850.1 40.940 447 246 10 19 457 50 486 4.08e-119 357
Msa0084330 AT3G52000.1 43.349 436 230 12 28 458 59 482 5.07e-117 352
Msa0084330 AT2G33530.1 43.304 448 232 12 22 455 23 462 3.78e-115 346
Msa0084330 AT3G52010.1 40.940 447 243 12 21 458 53 487 5.04e-115 347
Msa0084330 AT2G12480.3 40.460 435 248 7 29 456 28 458 2.02e-111 337
Msa0084330 AT1G43780.1 42.022 445 236 11 30 458 35 473 5.45e-111 336
Msa0084330 AT2G24000.2 42.377 446 182 9 22 457 20 400 3.00e-109 329
Msa0084330 AT5G42240.1 40.940 447 239 10 29 458 29 467 2.29e-108 329
Msa0084330 AT1G28110.1 40.639 438 238 11 31 455 30 458 1.22e-107 327
Msa0084330 AT1G28110.2 40.639 438 238 11 31 455 30 458 1.22e-107 327
Msa0084330 AT2G12480.4 38.940 434 232 8 29 456 28 434 7.55e-104 317
Msa0084330 AT5G23210.4 47.009 351 158 7 88 412 8 356 7.83e-103 311
Msa0084330 AT5G42230.1 38.926 447 248 10 29 458 25 463 1.06e-102 314
Msa0084330 AT5G42230.2 38.926 447 248 10 29 458 25 463 1.06e-102 314
Msa0084330 AT2G12480.1 38.940 434 230 8 29 456 28 432 2.13e-102 313
Msa0084330 AT2G12480.2 38.851 435 230 9 29 456 28 433 1.06e-100 308
Msa0084330 AT4G12910.1 35.152 495 266 12 9 454 9 497 4.61e-81 259
Msa0084330 AT3G25420.1 34.756 492 264 14 14 454 9 494 8.52e-81 259
Msa0084330 AT3G56540.1 61.111 180 65 4 10 188 57 232 3.19e-68 219
Msa0084330 AT4G12910.2 33.775 453 246 11 9 413 9 455 2.19e-67 223
Msa0084330 AT2G22990.1 31.871 433 262 14 33 454 23 433 2.53e-57 196
Msa0084330 AT2G22970.1 31.871 433 260 15 33 454 25 433 9.09e-57 194
Msa0084330 AT2G22920.2 31.178 433 265 14 33 454 25 435 1.75e-56 193
Msa0084330 AT1G73270.2 31.797 434 259 13 33 454 33 441 6.04e-54 187
Msa0084330 AT1G73270.1 31.797 434 259 13 33 454 33 441 7.08e-54 187
Msa0084330 AT1G73290.1 30.734 436 259 13 33 454 32 438 1.06e-51 181
Msa0084330 AT2G22990.5 30.556 432 260 14 33 454 23 424 1.37e-51 180
Msa0084330 AT1G73280.1 31.435 439 254 15 33 454 33 441 1.55e-51 180
Msa0084330 AT2G22990.4 31.941 407 245 13 33 428 23 408 2.62e-51 179
Msa0084330 AT2G22990.3 31.863 408 246 13 33 429 23 409 2.63e-51 180
Msa0084330 AT2G23000.1 28.406 433 279 13 33 454 25 437 1.51e-50 177
Msa0084330 AT2G23000.2 28.406 433 279 13 33 454 25 437 1.51e-50 177
Msa0084330 AT2G22970.3 31.100 418 254 14 33 439 25 419 3.47e-50 177
Msa0084330 AT3G12203.1 30.193 467 283 16 1 454 1 437 5.87e-50 176
Msa0084330 AT3G10450.1 30.162 431 270 11 33 454 29 437 8.43e-50 176
Msa0084330 AT2G23010.1 28.540 452 290 14 15 454 7 437 1.19e-49 175
Msa0084330 AT3G12203.3 30.716 433 264 14 33 454 9 416 1.64e-49 174
Msa0084330 AT2G22970.4 31.373 408 246 14 33 429 25 409 1.67e-49 174
Msa0084330 AT1G73300.1 30.365 438 260 15 33 454 33 441 4.16e-49 174
Msa0084330 AT1G73300.2 30.365 438 260 15 33 454 33 441 4.16e-49 174
Msa0084330 AT2G23010.2 28.319 452 291 14 15 454 7 437 4.91e-49 174
Msa0084330 AT2G22980.1 30.093 432 255 16 33 449 26 425 5.63e-48 171
Msa0084330 AT5G36180.1 30.365 438 260 15 33 454 33 441 2.18e-47 169
Msa0084330 AT2G22920.1 30.867 392 240 11 33 414 25 395 3.54e-47 168
Msa0084330 AT3G12220.1 30.184 434 262 13 33 452 27 433 4.13e-47 168
Msa0084330 AT5G22980.1 29.078 423 261 15 45 452 96 494 4.09e-46 167
Msa0084330 AT3G10450.2 29.782 413 259 11 51 454 4 394 4.99e-46 164
Msa0084330 AT1G33540.1 30.201 447 276 15 33 454 29 464 5.58e-46 166
Msa0084330 AT5G09640.1 27.479 484 292 14 6 454 6 465 4.93e-45 163
Msa0084330 AT3G12240.1 28.833 437 268 16 33 454 28 436 1.33e-44 162
Msa0084330 AT3G12203.2 30.446 404 245 14 62 454 1 379 1.55e-44 160
Msa0084330 AT3G12230.1 28.966 435 270 14 33 454 27 435 2.40e-44 161
Msa0084330 AT3G45010.1 28.140 430 272 12 38 452 92 499 5.26e-44 161
Msa0084330 AT1G33540.2 30.070 429 264 15 51 454 4 421 5.64e-44 159
Msa0084330 AT1G73290.3 30.886 395 232 11 33 414 32 398 6.40e-44 159
Msa0084330 AT1G73310.1 28.935 432 274 13 33 454 33 441 1.19e-43 159
Msa0084330 AT3G12220.2 29.567 416 252 13 51 452 15 403 2.79e-43 157
Msa0084330 AT3G10450.4 29.803 406 255 10 33 429 29 413 6.11e-43 157
Msa0084330 AT2G22980.4 29.147 422 253 15 33 439 26 416 9.70e-43 156
Msa0084330 AT3G10410.1 28.571 434 261 15 38 451 90 494 1.16e-42 158
Msa0084330 AT2G22980.2 29.612 412 244 15 33 429 26 406 2.74e-42 155
Msa0084330 AT2G22990.6 30.861 337 209 10 123 454 2 319 7.47e-41 149
Msa0084330 AT2G22990.2 30.564 337 210 10 123 454 2 319 2.42e-40 147
Msa0084330 AT3G10450.3 29.381 388 244 10 51 429 4 370 3.75e-39 145
Msa0084330 AT1G73310.2 29.280 403 254 11 33 426 33 413 2.25e-38 144
Msa0084330 AT2G22920.4 32.039 309 180 10 33 331 25 313 5.32e-38 141
Msa0084330 AT1G73280.2 34.201 269 158 8 33 286 33 297 1.62e-37 141
Msa0084330 AT2G22970.2 32.362 309 178 11 33 331 25 312 4.62e-37 138
Msa0084330 AT1G73290.2 33.333 267 161 7 33 286 32 294 1.79e-36 136
Msa0084330 AT3G12220.3 28.972 428 223 15 33 452 27 381 1.12e-35 136
Msa0084330 AT1G73280.4 29.014 355 213 12 112 454 12 339 9.24e-34 130
Msa0084330 AT2G22980.3 31.716 268 164 7 33 290 26 284 1.87e-32 127
Msa0084330 AT1G73280.3 28.739 341 205 11 125 454 1 314 3.11e-32 125
Msa0084330 AT1G15000.1 27.869 427 264 14 49 458 37 436 3.17e-30 122
Msa0084330 AT5G36180.5 28.249 354 217 12 112 454 12 339 1.17e-29 119
Msa0084330 AT5G36180.3 28.249 354 217 12 112 454 12 339 1.17e-29 119
Msa0084330 AT2G22980.5 27.532 316 192 11 123 429 2 289 5.83e-26 107
Msa0084330 AT5G22960.1 32.000 200 101 5 11 210 10 174 1.51e-24 100
Msa0084330 AT5G36180.2 27.660 329 202 11 112 429 12 315 3.48e-24 103
Msa0084330 AT5G36180.4 27.378 347 205 12 112 436 12 333 3.54e-23 100
Msa0084330 AT2G22920.3 26.838 272 176 8 188 454 3 256 9.83e-23 97.4
Msa0084330 AT1G73310.3 26.959 319 210 8 112 426 12 311 4.50e-22 97.1
Msa0084330 AT2G27920.1 23.718 468 300 22 9 442 5 449 1.83e-15 79.0
Msa0084330 AT2G22960.1 30.147 136 90 4 320 454 53 184 1.94e-12 66.2

Find 131 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATTTAGTTATCTGTTTC+TGG 0.114317 1_2:+63677959 None:intergenic
TAAAACTAATGGCCTTAAAT+TGG 0.173728 1_2:+63677392 None:intergenic
TAACAGGGATTCCATATATT+TGG 0.181948 1_2:-63675936 Msa0084330:CDS
GTGATATTGTTGGGAATTAT+TGG 0.185000 1_2:-63675808 Msa0084330:CDS
ACTTGTTTACCAGAGAAATA+AGG 0.220874 1_2:-63676549 Msa0084330:CDS
TGAAATTCCAACACAGAAAA+TGG 0.225573 1_2:+63677578 None:intergenic
GGTTGTTCTTCAATTGCTTA+TGG 0.253357 1_2:-63677351 Msa0084330:CDS
TGTTCAAATCCAACATTCTT+TGG 0.267824 1_2:+63677498 None:intergenic
CTGCATGTCTTCCAAATATA+TGG 0.277840 1_2:+63675925 None:intergenic
TGAAGCGCCTTCTGCACTTC+TGG 0.279971 1_2:+63675967 None:intergenic
GTTGGATTTGAACAGTATTC+AGG 0.287775 1_2:-63677489 Msa0084330:CDS
AAACAAAACAATATAGAATA+TGG 0.298366 1_2:+63677605 None:intergenic
TGAACCGTGGACCCAATTTA+AGG 0.299323 1_2:-63677404 Msa0084330:CDS
CTAAGAATTGCTTGTGACTT+TGG 0.299740 1_2:-63676288 Msa0084330:CDS
GCGCTTCATGCCAATGTAAC+AGG 0.306670 1_2:-63675952 Msa0084330:CDS
TATGGTGCTTCTGAAGAAAT+TGG 0.325012 1_2:-63677333 Msa0084330:CDS
TTCAATTGAAGAACTACTAT+TGG 0.325026 1_2:+63674465 None:intergenic
GTCTCCGGGTATGGGTATTC+AGG 0.330012 1_2:-63675733 Msa0084330:intron
TTTCTGTGTTGGAATTTCAT+TGG 0.334125 1_2:-63677574 Msa0084330:CDS
AATCCCTTAAAATTGATAAC+TGG 0.337875 1_2:+63676515 None:intergenic
GGGATGTGTATTCCTAGATT+TGG 0.339355 1_2:-63677715 Msa0084330:five_prime_UTR
TGTCAATTCCCTTATTTCTC+TGG 0.354954 1_2:+63676540 None:intergenic
CATAGAGAATTCTACATTGC+TGG 0.368294 1_2:-63676728 Msa0084330:CDS
AACTTATTAACGCTGGTCTC+CGG 0.369243 1_2:-63675748 Msa0084330:CDS
ATTTATCAAATTTAATTTCC+AGG 0.369979 1_2:+63674491 None:intergenic
TTAACGCTGGTCTCCGGGTA+TGG 0.373121 1_2:-63675742 Msa0084330:CDS
TATCAAACTTTCCATCTTTG+AGG 0.379502 1_2:+63678060 None:intergenic
CTTGTTTACCAGAGAAATAA+GGG 0.385746 1_2:-63676548 Msa0084330:CDS
GGATGGAGTCAAGTTTATAA+AGG 0.388461 1_2:-63674779 Msa0084330:CDS
GAACAGGCTGAGACAGAATA+AGG 0.395226 1_2:-63674549 Msa0084330:three_prime_UTR
GGCGGAGTATAAATGCTATA+TGG 0.395551 1_2:+63676192 None:intergenic
CTGTTCCTTGATTCTCCTGC+TGG 0.401847 1_2:-63677132 Msa0084330:CDS
CTTGATTCTCCTGCTGGTGT+TGG 0.404698 1_2:-63677126 Msa0084330:CDS
AAATTCAAAGCATTCATGAT+TGG 0.405270 1_2:-63677943 Msa0084330:five_prime_UTR
TCTCAGCCTGTTCCATGTAT+AGG 0.406780 1_2:+63674559 None:intergenic
TGATTTCAGATTCTACATAT+AGG 0.409759 1_2:-63676314 Msa0084330:CDS
TTCTTGACAGTGATATTGTT+GGG 0.410531 1_2:-63675817 Msa0084330:intron
TACTCTTCTGTGAAGGTTGG+TGG 0.412687 1_2:-63674800 Msa0084330:intron
AAAACTAATGGCCTTAAATT+GGG 0.416290 1_2:+63677393 None:intergenic
TTAATAAGTTCATGATATAT+AGG 0.416930 1_2:+63675761 None:intergenic
AAGTTATTCTTAAGAATGTA+AGG 0.417353 1_2:+63674587 None:intergenic
TAAACTTTGCATTTAGCTCC+TGG 0.418833 1_2:-63674340 Msa0084330:three_prime_UTR
CCATCATCAATTGGTATCCT+TGG 0.423024 1_2:-63674924 Msa0084330:CDS
AAGAAAAGTGATTTGCCATC+AGG 0.425397 1_2:+63677294 None:intergenic
CTATCAACTATGTATGCATT+TGG 0.430352 1_2:+63677911 None:intergenic
TAATAACATCGTTATTATTG+AGG 0.448081 1_2:+63678112 None:intergenic
TTATGGCTTAATGGTGGTCC+TGG 0.448568 1_2:-63677372 Msa0084330:CDS
TCAATTCCTATACATGGAAC+AGG 0.448805 1_2:-63674565 Msa0084330:three_prime_UTR
GATGATGGTTGGTAGCTTCA+AGG 0.449314 1_2:+63674939 None:intergenic
ACAACAGATCTGTACACATT+TGG 0.450890 1_2:-63677084 Msa0084330:CDS
TTTCTTGACAGTGATATTGT+TGG 0.454235 1_2:-63675818 Msa0084330:intron
AAGAATCAAAGACCAAATCT+AGG 0.457192 1_2:+63677703 None:intergenic
ACGATTATCATGACTATGTT+GGG 0.461685 1_2:-63676365 Msa0084330:CDS
CCAACATTCTTTGGTTGCCC+TGG 0.461852 1_2:+63677507 None:intergenic
CCATTAAGCCATAAAACTAA+TGG 0.465147 1_2:+63677381 None:intergenic
ACATTTGGTGACCAGAAAAC+AGG 0.468417 1_2:-63677069 Msa0084330:intron
TTCCATCTTGCATCTCTGCC+TGG 0.468808 1_2:-63674509 Msa0084330:three_prime_UTR
GACGATTATCATGACTATGT+TGG 0.470565 1_2:-63676366 Msa0084330:CDS
ATATAGGAAGCATAGACAAT+GGG 0.471053 1_2:+63675777 None:intergenic
CATATATTTGGAAGACATGC+AGG 0.472543 1_2:-63675924 Msa0084330:intron
CTCACATTGGTAACAATAAG+AGG 0.473526 1_2:-63674755 Msa0084330:CDS
ATAGAGAATTCTACATTGCT+GGG 0.479532 1_2:-63676727 Msa0084330:CDS
AGCACTAATGATTGACATCC+AGG 0.482811 1_2:+63674322 None:intergenic
GCTTCTCATTAGCTTGATGA+TGG 0.483315 1_2:+63674653 None:intergenic
AAGCGAGAGACATAAAACTA+TGG 0.483404 1_2:+63677816 None:intergenic
AGCTACCAACCATCATCAAT+TGG 0.485261 1_2:-63674933 Msa0084330:CDS
ATATGGAATCCCTGTTACAT+TGG 0.488606 1_2:+63675942 None:intergenic
GCCTGAAAACATTGCACTGA+TGG 0.488616 1_2:+63676252 None:intergenic
AGCACTTCAATAAATTCCAT+TGG 0.490179 1_2:-63677677 Msa0084330:five_prime_UTR
ACTTATTAACGCTGGTCTCC+GGG 0.492860 1_2:-63675747 Msa0084330:CDS
TAACGCTGGTCTCCGGGTAT+GGG 0.495844 1_2:-63675741 Msa0084330:CDS
CAACACCAGCAGGAGAATCA+AGG 0.496627 1_2:+63677127 None:intergenic
GATGGAGTCAAGTTTATAAA+GGG 0.496669 1_2:-63674778 Msa0084330:CDS
AGGTCATTATGTTCCTCAGT+TGG 0.498140 1_2:-63676576 Msa0084330:CDS
GATAAGATTAAGCAGTTACC+AGG 0.504649 1_2:-63677525 Msa0084330:CDS
TGGTATCCTTGGTATGATAG+TGG 0.505825 1_2:-63674913 Msa0084330:CDS
ATCTATCTTAGCCTCAAAGA+TGG 0.518994 1_2:-63678071 Msa0084330:five_prime_UTR
TTGGTAACAATAAGAGGTGC+TGG 0.519871 1_2:-63674749 Msa0084330:CDS
GAATATCGAGTTGCGGTCAG+TGG 0.520379 1_2:+63674970 None:intergenic
ATGTTGGGACATTTGAGTAC+TGG 0.520555 1_2:-63676350 Msa0084330:CDS
TAGATCATTCTTGGAGAACA+AGG 0.525636 1_2:-63674681 Msa0084330:CDS
CCAGGGCAACCAAAGAATGT+TGG 0.526279 1_2:-63677507 Msa0084330:CDS
CTTCTGTGAAGGTTGGTGGA+TGG 0.542653 1_2:-63674796 Msa0084330:intron
CCATCAGGCCTAATACGAAA+AGG 0.545705 1_2:+63677309 None:intergenic
ATACACGTCAGAATGCCTTT+CGG 0.549353 1_2:+63675999 None:intergenic
TTGGTGCTACAGGTTGGAAA+TGG 0.551086 1_2:-63676396 Msa0084330:intron
TCACTCAGAAGCGGCTTGAA+CGG 0.556715 1_2:-63676153 Msa0084330:CDS
ATTGCTGGGGAAAGTTATGC+AGG 0.557240 1_2:-63676713 Msa0084330:intron
GGATACCAATTGATGATGGT+TGG 0.557745 1_2:+63674928 None:intergenic
ATAACTTCAATTCCTATACA+TGG 0.558875 1_2:-63674571 Msa0084330:three_prime_UTR
TCAGAATGCCTTTCGGTACA+CGG 0.563005 1_2:+63676006 None:intergenic
CCAAGGATACCAATTGATGA+TGG 0.563122 1_2:+63674924 None:intergenic
CTAATGAATGAGAGAACCAA+TGG 0.563685 1_2:+63677661 None:intergenic
AGAGTTGCAGTTGCTGAGCA+AGG 0.568738 1_2:-63676225 Msa0084330:CDS
CGCTTCATGCCAATGTAACA+GGG 0.570208 1_2:-63675951 Msa0084330:CDS
TTCCAGGCAGAGATGCAAGA+TGG 0.576330 1_2:+63674507 None:intergenic
TTTGAGTACTGGTGGACACA+TGG 0.597648 1_2:-63676339 Msa0084330:CDS
AGTGATGCGGTATTGTTACA+AGG 0.603572 1_2:+63676171 None:intergenic
AAATTGGGTCCACGGTTCAG+TGG 0.604072 1_2:+63677408 None:intergenic
CTGTGAATGAACAGAATGGA+AGG 0.605445 1_2:-63677458 Msa0084330:CDS
GAGTTGTTGAGTGGTGCACA+TGG 0.606168 1_2:+63678033 None:intergenic
TCATATGCTCTAGACATCCA+TGG 0.607409 1_2:+63676030 None:intergenic
CAATCGTCCAGAAGTGCAGA+AGG 0.609778 1_2:-63675974 Msa0084330:CDS
TATATAGGAAGCATAGACAA+TGG 0.610027 1_2:+63675776 None:intergenic
ATGGCCTTAAATTGGGTCCA+CGG 0.610231 1_2:+63677400 None:intergenic
GCTTCGCGGGGCTTATGAAG+AGG 0.611657 1_2:+63674716 None:intergenic
TATCATGAACTTATTAACGC+TGG 0.615510 1_2:-63675755 Msa0084330:CDS
GGAACAAGGCTAAAACTATG+AGG 0.616268 1_2:+63677793 None:intergenic
GTGACTGTGAATGAACAGAA+TGG 0.619017 1_2:-63677462 Msa0084330:CDS
CATATGATCCGTGTACCGAA+AGG 0.636629 1_2:-63676014 Msa0084330:CDS
AAAAGAATAAACGCTTCGCG+GGG 0.640750 1_2:+63674704 None:intergenic
CGTACCTGAATACCCATACC+CGG 0.640864 1_2:+63675729 None:intergenic
ATAAGATTAAGCAGTTACCA+GGG 0.641370 1_2:-63677524 Msa0084330:CDS
AATTCTCTATGCTTATACTG+AGG 0.645681 1_2:+63676740 None:intergenic
TTGGGACATTTGAGTACTGG+TGG 0.645881 1_2:-63676347 Msa0084330:CDS
ATCGATAGAATATCGAGTTG+CGG 0.654875 1_2:+63674963 None:intergenic
AGTTTATAAAGGGCTCACAT+TGG 0.657748 1_2:-63674768 Msa0084330:CDS
AATAAGAGGTGCTGGACACG+AGG 0.657878 1_2:-63674741 Msa0084330:CDS
TGACTGAGTGAGTTGTTGAG+TGG 0.657980 1_2:+63678024 None:intergenic
TCCTTGGTATGATAGTGGCA+AGG 0.660717 1_2:-63674908 Msa0084330:intron
ACCTTGCCACTATCATACCA+AGG 0.669971 1_2:+63674907 None:intergenic
TAGAGAATTCTACATTGCTG+GGG 0.672609 1_2:-63676726 Msa0084330:CDS
TAAGCAATTGAAGAACAACC+AGG 0.684683 1_2:+63677354 None:intergenic
AATACCGCATCACTCAGAAG+CGG 0.685301 1_2:-63676162 Msa0084330:CDS
ATTGAAGCACCACTGAACCG+TGG 0.692357 1_2:-63677417 Msa0084330:CDS
TATAGGAAGCATAGACAATG+GGG 0.694959 1_2:+63675778 None:intergenic
AGTTGTTGAGTGGTGCACAT+GGG 0.696031 1_2:+63678034 None:intergenic
CAAGCCGCTTCTGAGTGATG+CGG 0.701434 1_2:+63676158 None:intergenic
TAAACAAGTTGAGCCAACTG+AGG 0.719538 1_2:+63676563 None:intergenic
TGTGAATGAACAGAATGGAA+GGG 0.721865 1_2:-63677457 Msa0084330:CDS
GATGCGGTATTGTTACAAGG+CGG 0.780235 1_2:+63676174 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTGTTTTATAGAATAA+TGG - chr1_2:63674729-63674748 Msa0084330:CDS 10.0%
!!! TTTTTGTTTTATAGAATAAT+GGG - chr1_2:63674730-63674749 Msa0084330:CDS 10.0%
!! AAACAAAACAATATAGAATA+TGG + chr1_2:63674761-63674780 None:intergenic 15.0%
!!! TTTATGCCTATTTATTATTT+TGG - chr1_2:63675191-63675210 Msa0084330:intron 15.0%
!!! TATCAGAATTTTGATTTAAA+AGG - chr1_2:63676509-63676528 Msa0084330:intron 15.0%
!! TTAATAAGTTCATGATATAT+AGG + chr1_2:63676605-63676624 None:intergenic 15.0%
!!! ACTTTTTTGCTTATATTTAT+CGG - chr1_2:63676684-63676703 Msa0084330:intron 15.0%
!!! ATTTATCAAATTTAATTTCC+AGG + chr1_2:63677875-63677894 None:intergenic 15.0%
!! TAATAACATCGTTATTATTG+AGG + chr1_2:63674254-63674273 None:intergenic 20.0%
!!! AGTTTTAGATCTTTTAGTAT+TGG - chr1_2:63674627-63674646 Msa0084330:three_prime_UTR 20.0%
!!! GTTTTAGATCTTTTAGTATT+GGG - chr1_2:63674628-63674647 Msa0084330:three_prime_UTR 20.0%
!! CAACATCCAAAATAATAAAT+AGG + chr1_2:63675200-63675219 None:intergenic 20.0%
!!! CATATATTTTTCTTGTAAAG+TGG - chr1_2:63675588-63675607 Msa0084330:intron 20.0%
!!! TATATATCAGTTTTCTACTT+TGG - chr1_2:63675688-63675707 Msa0084330:intron 20.0%
!! ATTCATTCAATTGATAAGTA+TGG + chr1_2:63675716-63675735 None:intergenic 20.0%
!!! TGAAAAATTGAATGATTTTC+AGG - chr1_2:63675767-63675786 Msa0084330:CDS 20.0%
!!! AAACCAGTTATCAATTTTAA+GGG - chr1_2:63675845-63675864 Msa0084330:intron 20.0%
!!! TATCAATTTTAAGGGATTTA+TGG - chr1_2:63675853-63675872 Msa0084330:intron 20.0%
!! ATGTATTTGGTATATAGTTA+AGG + chr1_2:63676662-63676681 None:intergenic 20.0%
!! ATAAACACAACAAAAGTAAT+GGG + chr1_2:63676888-63676907 None:intergenic 20.0%
!! AATAAACACAACAAAAGTAA+TGG + chr1_2:63676889-63676908 None:intergenic 20.0%
!!! AAGGAACAAAGATATTTTTT+TGG + chr1_2:63676961-63676980 None:intergenic 20.0%
!! ATATTATGATGTGATTTGAT+TGG + chr1_2:63677197-63677216 None:intergenic 20.0%
!!! TCTTCTTAACTTTGATATTT+TGG - chr1_2:63677342-63677361 Msa0084330:CDS 20.0%
!!! TGTTCAAGTTTATACTAATT+TGG - chr1_2:63677509-63677528 Msa0084330:CDS 20.0%
!!! TATTCTTTTTAGATCATTCT+TGG - chr1_2:63677673-63677692 Msa0084330:five_prime_UTR 20.0%
!! AAGTTATTCTTAAGAATGTA+AGG + chr1_2:63677779-63677798 None:intergenic 20.0%
! TGAATTTAGTTATCTGTTTC+TGG + chr1_2:63674407-63674426 None:intergenic 25.0%
!! AAATTCAAAGCATTCATGAT+TGG - chr1_2:63674420-63674439 Msa0084330:three_prime_UTR 25.0%
!! AAAACTAATGGCCTTAAATT+GGG + chr1_2:63674973-63674992 None:intergenic 25.0%
!! TAAAACTAATGGCCTTAAAT+TGG + chr1_2:63674974-63674993 None:intergenic 25.0%
!!! ATTTAAGGCCATTAGTTTTA+TGG - chr1_2:63674974-63674993 Msa0084330:CDS 25.0%
!! CAAACCAGTTATCAATTTTA+AGG - chr1_2:63675844-63675863 Msa0084330:intron 25.0%
!! AATCCCTTAAAATTGATAAC+TGG + chr1_2:63675851-63675870 None:intergenic 25.0%
! GTTATATGATTATCATAAGG+TGG + chr1_2:63675928-63675947 None:intergenic 25.0%
! CTCGTTATATGATTATCATA+AGG + chr1_2:63675931-63675950 None:intergenic 25.0%
! TGATTTCAGATTCTACATAT+AGG - chr1_2:63676049-63676068 Msa0084330:intron 25.0%
! TCAATAAATCGATAGCATAT+AGG + chr1_2:63676248-63676267 None:intergenic 25.0%
!!! TATGCTATCGATTTATTGAA+TGG - chr1_2:63676249-63676268 Msa0084330:CDS 25.0%
! AACAAATTAAACACAGTTGA+TGG + chr1_2:63676292-63676311 None:intergenic 25.0%
!! AGCAAAAAAGTACATGTATT+TGG + chr1_2:63676675-63676694 None:intergenic 25.0%
!! TAGTATGTGATATCAACTTT+TGG - chr1_2:63676802-63676821 Msa0084330:intron 25.0%
! TAGAGAAATATGAAATGGAT+GGG + chr1_2:63677019-63677038 None:intergenic 25.0%
! TTAGAGAAATATGAAATGGA+TGG + chr1_2:63677020-63677039 None:intergenic 25.0%
! GCATTTAGAGAAATATGAAA+TGG + chr1_2:63677024-63677043 None:intergenic 25.0%
! CAATTTGTACTAGTAACATT+CGG - chr1_2:63677088-63677107 Msa0084330:CDS 25.0%
! AATTTGTACTAGTAACATTC+GGG - chr1_2:63677089-63677108 Msa0084330:CDS 25.0%
! TCATTGATCTATACAGAATA+TGG + chr1_2:63677137-63677156 None:intergenic 25.0%
! TCTGTATAGATCAATGATTA+TGG - chr1_2:63677140-63677159 Msa0084330:CDS 25.0%
! TATCTCAATAAATATCCACA+TGG - chr1_2:63677242-63677261 Msa0084330:intron 25.0%
! TCTTATCTGTAATCAAGAAT+TGG - chr1_2:63677316-63677335 Msa0084330:CDS 25.0%
!! TAAATTTTACTCTTCTGTGA+AGG - chr1_2:63677556-63677575 Msa0084330:CDS 25.0%
!!! AAGAATGTAAGGTACTTTTT+TGG + chr1_2:63677768-63677787 None:intergenic 25.0%
! ATAACTTCAATTCCTATACA+TGG - chr1_2:63677792-63677811 Msa0084330:five_prime_UTR 25.0%
! TTCAATTGAAGAACTACTAT+TGG + chr1_2:63677901-63677920 None:intergenic 25.0%
TATCAAACTTTCCATCTTTG+AGG + chr1_2:63674306-63674325 None:intergenic 30.0%
CTATCAACTATGTATGCATT+TGG + chr1_2:63674455-63674474 None:intergenic 30.0%
ATAGAGAAAAACAAGGAACA+AGG + chr1_2:63674587-63674606 None:intergenic 30.0%
AAGAATCAAAGACCAAATCT+AGG + chr1_2:63674663-63674682 None:intergenic 30.0%
AGCACTTCAATAAATTCCAT+TGG - chr1_2:63674686-63674705 Msa0084330:CDS 30.0%
TGAAATTCCAACACAGAAAA+TGG + chr1_2:63674788-63674807 None:intergenic 30.0%
TTTCTGTGTTGGAATTTCAT+TGG - chr1_2:63674789-63674808 Msa0084330:intron 30.0%
ATAAGATTAAGCAGTTACCA+GGG - chr1_2:63674839-63674858 Msa0084330:intron 30.0%
TGTTCAAATCCAACATTCTT+TGG + chr1_2:63674868-63674887 None:intergenic 30.0%
CCATTAAGCCATAAAACTAA+TGG + chr1_2:63674985-63675004 None:intergenic 30.0%
!!! CCATTAGTTTTATGGCTTAA+TGG - chr1_2:63674982-63675001 Msa0084330:CDS 30.0%
!!! TTAGTTTTATGGCTTAATGG+TGG - chr1_2:63674985-63675004 Msa0084330:CDS 30.0%
! AAATTGGTCCTTTTCGTATT+AGG - chr1_2:63675046-63675065 Msa0084330:intron 30.0%
! TTTTCTTGAATCGTTATGCT+TGG - chr1_2:63675085-63675104 Msa0084330:intron 30.0%
AATTCTCTATGCTTATACTG+AGG + chr1_2:63675626-63675645 None:intergenic 30.0%
ATAGAGAATTCTACATTGCT+GGG - chr1_2:63675636-63675655 Msa0084330:intron 30.0%
ACTTGTTTACCAGAGAAATA+AGG - chr1_2:63675814-63675833 Msa0084330:intron 30.0%
CTTGTTTACCAGAGAAATAA+GGG - chr1_2:63675815-63675834 Msa0084330:intron 30.0%
GGATTTATGGTAAGATTGTT+AGG - chr1_2:63675866-63675885 Msa0084330:intron 30.0%
GAGTTTGATTATTCTGTGAT+TGG - chr1_2:63675948-63675967 Msa0084330:CDS 30.0%
ACGATTATCATGACTATGTT+GGG - chr1_2:63675998-63676017 Msa0084330:CDS 30.0%
TCTTATAAAACAAACAGCCA+TGG - chr1_2:63676316-63676335 Msa0084330:CDS 30.0%
TAACAGGGATTCCATATATT+TGG - chr1_2:63676427-63676446 Msa0084330:intron 30.0%
TTTCTTGACAGTGATATTGT+TGG - chr1_2:63676545-63676564 Msa0084330:CDS 30.0%
TTCTTGACAGTGATATTGTT+GGG - chr1_2:63676546-63676565 Msa0084330:CDS 30.0%
GTGATATTGTTGGGAATTAT+TGG - chr1_2:63676555-63676574 Msa0084330:CDS 30.0%
! ATATAGGAAGCATAGACAAT+GGG + chr1_2:63676589-63676608 None:intergenic 30.0%
! TATATAGGAAGCATAGACAA+TGG + chr1_2:63676590-63676609 None:intergenic 30.0%
TATCATGAACTTATTAACGC+TGG - chr1_2:63676608-63676627 Msa0084330:intron 30.0%
TTGATATCACATACTATCCA+TGG + chr1_2:63676798-63676817 None:intergenic 30.0%
AAGTGACACTCAAATGATTA+AGG + chr1_2:63676980-63676999 None:intergenic 30.0%
AATGCTCTTTGACATTATTG+AGG - chr1_2:63677039-63677058 Msa0084330:intron 30.0%
ACAGAATATGGAGTAATGAA+AGG + chr1_2:63677125-63677144 None:intergenic 30.0%
GATGGAGTCAAGTTTATAAA+GGG - chr1_2:63677585-63677604 Msa0084330:CDS 30.0%
TAAAAAGAATAAACGCTTCG+CGG + chr1_2:63677664-63677683 None:intergenic 30.0%
ATCTATCTTAGCCTCAAAGA+TGG - chr1_2:63674292-63674311 Msa0084330:three_prime_UTR 35.0%
AAGCGAGAGACATAAAACTA+TGG + chr1_2:63674550-63674569 None:intergenic 35.0%
!!! TTCCTTGTTTTTCTCTATGC+AGG - chr1_2:63674589-63674608 Msa0084330:three_prime_UTR 35.0%
CTAATGAATGAGAGAACCAA+TGG + chr1_2:63674705-63674724 None:intergenic 35.0%
! TTTGTGTCCATTTTCTGTGT+TGG - chr1_2:63674778-63674797 Msa0084330:CDS 35.0%
GATAAGATTAAGCAGTTACC+AGG - chr1_2:63674838-63674857 Msa0084330:intron 35.0%
GTTGGATTTGAACAGTATTC+AGG - chr1_2:63674874-63674893 Msa0084330:intron 35.0%
TGTGAATGAACAGAATGGAA+GGG - chr1_2:63674906-63674925 Msa0084330:intron 35.0%
TAAGCAATTGAAGAACAACC+AGG + chr1_2:63675012-63675031 None:intergenic 35.0%
GGTTGTTCTTCAATTGCTTA+TGG - chr1_2:63675012-63675031 Msa0084330:intron 35.0%
! TATGGTGCTTCTGAAGAAAT+TGG - chr1_2:63675030-63675049 Msa0084330:intron 35.0%
! AAGAAAAGTGATTTGCCATC+AGG + chr1_2:63675072-63675091 None:intergenic 35.0%
CAAAGACTGAATCTCAGAAT+CGG + chr1_2:63675124-63675143 None:intergenic 35.0%
ACAACAGATCTGTACACATT+TGG - chr1_2:63675279-63675298 Msa0084330:intron 35.0%
! TACATTACCTACCTGTTTTC+TGG + chr1_2:63675308-63675327 None:intergenic 35.0%
TGTAATGATCATGCTCATGA+AGG - chr1_2:63675459-63675478 Msa0084330:intron 35.0%
TTGCTAAAGCAAAACAAAGC+TGG + chr1_2:63675499-63675518 None:intergenic 35.0%
CATAGAGAATTCTACATTGC+TGG - chr1_2:63675635-63675654 Msa0084330:intron 35.0%
TAGAGAATTCTACATTGCTG+GGG - chr1_2:63675637-63675656 Msa0084330:intron 35.0%
! TGTCAATTCCCTTATTTCTC+TGG + chr1_2:63675826-63675845 None:intergenic 35.0%
GACGATTATCATGACTATGT+TGG - chr1_2:63675997-63676016 Msa0084330:CDS 35.0%
CTAAGAATTGCTTGTGACTT+TGG - chr1_2:63676075-63676094 Msa0084330:intron 35.0%
ATATGGAATCCCTGTTACAT+TGG + chr1_2:63676424-63676443 None:intergenic 35.0%
CTGCATGTCTTCCAAATATA+TGG + chr1_2:63676441-63676460 None:intergenic 35.0%
CATATATTTGGAAGACATGC+AGG - chr1_2:63676439-63676458 Msa0084330:intron 35.0%
ATGTTGAACACGTTCTATTG+AGG - chr1_2:63676479-63676498 Msa0084330:intron 35.0%
! TATAGGAAGCATAGACAATG+GGG + chr1_2:63676588-63676607 None:intergenic 35.0%
TTGACATCTGAATACATCCA+TGG - chr1_2:63676778-63676797 Msa0084330:CDS 35.0%
! ATCGATAGAATATCGAGTTG+CGG + chr1_2:63677403-63677422 None:intergenic 35.0%
! TTTTACTCTTCTGTGAAGGT+TGG - chr1_2:63677560-63677579 Msa0084330:CDS 35.0%
GGATGGAGTCAAGTTTATAA+AGG - chr1_2:63677584-63677603 Msa0084330:CDS 35.0%
AGTTTATAAAGGGCTCACAT+TGG - chr1_2:63677595-63677614 Msa0084330:CDS 35.0%
! CTCACATTGGTAACAATAAG+AGG - chr1_2:63677608-63677627 Msa0084330:CDS 35.0%
AAAAAGAATAAACGCTTCGC+GGG + chr1_2:63677663-63677682 None:intergenic 35.0%
TAGATCATTCTTGGAGAACA+AGG - chr1_2:63677682-63677701 Msa0084330:five_prime_UTR 35.0%
TCAATTCCTATACATGGAAC+AGG - chr1_2:63677798-63677817 Msa0084330:five_prime_UTR 35.0%
! TAAACTTTGCATTTAGCTCC+TGG - chr1_2:63678023-63678042 Msa0084330:five_prime_UTR 35.0%
GGAACAAGGCTAAAACTATG+AGG + chr1_2:63674573-63674592 None:intergenic 40.0%
TGCCTGCATAGAGAAAAACA+AGG + chr1_2:63674594-63674613 None:intergenic 40.0%
GGGATGTGTATTCCTAGATT+TGG - chr1_2:63674648-63674667 Msa0084330:exon 40.0%
GTGACTGTGAATGAACAGAA+TGG - chr1_2:63674901-63674920 Msa0084330:intron 40.0%
CTGTGAATGAACAGAATGGA+AGG - chr1_2:63674905-63674924 Msa0084330:intron 40.0%
TAAGAAAAACCAACACCAGC+AGG + chr1_2:63675249-63675268 None:intergenic 40.0%
! ACATTTGGTGACCAGAAAAC+AGG - chr1_2:63675294-63675313 Msa0084330:intron 40.0%
CGTTAGTGACACATTGACAT+TGG - chr1_2:63675365-63675384 Msa0084330:intron 40.0%
! TTGAGTTTTACCACGTGTGT+CGG - chr1_2:63675412-63675431 Msa0084330:intron 40.0%
GCTCATGAAGGATTTACTCA+TGG - chr1_2:63675471-63675490 Msa0084330:intron 40.0%
AGGTCATTATGTTCCTCAGT+TGG - chr1_2:63675787-63675806 Msa0084330:CDS 40.0%
! TAAACAAGTTGAGCCAACTG+AGG + chr1_2:63675803-63675822 None:intergenic 40.0%
!! TATTCTGTGATTGGTGCTAC+AGG - chr1_2:63675957-63675976 Msa0084330:CDS 40.0%
ATGTTGGGACATTTGAGTAC+TGG - chr1_2:63676013-63676032 Msa0084330:CDS 40.0%
! TCCATCAGTGCAATGTTTTC+AGG - chr1_2:63676110-63676129 Msa0084330:intron 40.0%
GGCGGAGTATAAATGCTATA+TGG + chr1_2:63676174-63676193 None:intergenic 40.0%
AGTGATGCGGTATTGTTACA+AGG + chr1_2:63676195-63676214 None:intergenic 40.0%
! TCATATGCTCTAGACATCCA+TGG + chr1_2:63676336-63676355 None:intergenic 40.0%
ATACACGTCAGAATGCCTTT+CGG + chr1_2:63676367-63676386 None:intergenic 40.0%
! AACTTATTAACGCTGGTCTC+CGG - chr1_2:63676615-63676634 Msa0084330:intron 40.0%
TTGACATTATTGAGGCTCAC+TGG - chr1_2:63677047-63677066 Msa0084330:intron 40.0%
!!! CTTTGATATTTTGGCTGCAG+TGG - chr1_2:63677351-63677370 Msa0084330:CDS 40.0%
AGCTACCAACCATCATCAAT+TGG - chr1_2:63677430-63677449 Msa0084330:CDS 40.0%
!! GGATACCAATTGATGATGGT+TGG + chr1_2:63677438-63677457 None:intergenic 40.0%
CCAAGGATACCAATTGATGA+TGG + chr1_2:63677442-63677461 None:intergenic 40.0%
CCATCATCAATTGGTATCCT+TGG - chr1_2:63677439-63677458 Msa0084330:CDS 40.0%
TGGTATCCTTGGTATGATAG+TGG - chr1_2:63677450-63677469 Msa0084330:CDS 40.0%
! TTGGTAACAATAAGAGGTGC+TGG - chr1_2:63677614-63677633 Msa0084330:CDS 40.0%
AAAAGAATAAACGCTTCGCG+GGG + chr1_2:63677662-63677681 None:intergenic 40.0%
! GCTTCTCATTAGCTTGATGA+TGG + chr1_2:63677713-63677732 None:intergenic 40.0%
AGCACTAATGATTGACATCC+AGG + chr1_2:63678044-63678063 None:intergenic 40.0%
AGTTGTTGAGTGGTGCACAT+GGG + chr1_2:63674332-63674351 None:intergenic 45.0%
TGACTGAGTGAGTTGTTGAG+TGG + chr1_2:63674342-63674361 None:intergenic 45.0%
!!! ATGGAAGGGCACTGTTTTAC+TGG - chr1_2:63674920-63674939 Msa0084330:CDS 45.0%
TGAACCGTGGACCCAATTTA+AGG - chr1_2:63674959-63674978 Msa0084330:CDS 45.0%
! ATGGCCTTAAATTGGGTCCA+CGG + chr1_2:63674966-63674985 None:intergenic 45.0%
TTATGGCTTAATGGTGGTCC+TGG - chr1_2:63674991-63675010 Msa0084330:CDS 45.0%
CCATCAGGCCTAATACGAAA+AGG + chr1_2:63675057-63675076 None:intergenic 45.0%
! CCTTTTCGTATTAGGCCTGA+TGG - chr1_2:63675054-63675073 Msa0084330:intron 45.0%
! TTGGTGACCAGAAAACAGGT+AGG - chr1_2:63675298-63675317 Msa0084330:intron 45.0%
! CAATGTGTCACTAACGTGTC+CGG + chr1_2:63675361-63675380 None:intergenic 45.0%
GTTTCTTGACTTTGCAGCTG+AGG - chr1_2:63675562-63675581 Msa0084330:intron 45.0%
ATTGCTGGGGAAAGTTATGC+AGG - chr1_2:63675650-63675669 Msa0084330:intron 45.0%
!! TTGGTGCTACAGGTTGGAAA+TGG - chr1_2:63675967-63675986 Msa0084330:CDS 45.0%
!! TTGGGACATTTGAGTACTGG+TGG - chr1_2:63676016-63676035 Msa0084330:CDS 45.0%
!! TTTGAGTACTGGTGGACACA+TGG - chr1_2:63676024-63676043 Msa0084330:CDS 45.0%
GCCTGAAAACATTGCACTGA+TGG + chr1_2:63676114-63676133 None:intergenic 45.0%
GATGCGGTATTGTTACAAGG+CGG + chr1_2:63676192-63676211 None:intergenic 45.0%
AATACCGCATCACTCAGAAG+CGG - chr1_2:63676201-63676220 Msa0084330:CDS 45.0%
CATATGATCCGTGTACCGAA+AGG - chr1_2:63676349-63676368 Msa0084330:CDS 45.0%
TCAGAATGCCTTTCGGTACA+CGG + chr1_2:63676360-63676379 None:intergenic 45.0%
CGCTTCATGCCAATGTAACA+GGG - chr1_2:63676412-63676431 Msa0084330:intron 45.0%
! ACTTATTAACGCTGGTCTCC+GGG - chr1_2:63676616-63676635 Msa0084330:intron 45.0%
!! GATGATGGTTGGTAGCTTCA+AGG + chr1_2:63677427-63677446 None:intergenic 45.0%
!! TCCTTGGTATGATAGTGGCA+AGG - chr1_2:63677455-63677474 Msa0084330:CDS 45.0%
ACCTTGCCACTATCATACCA+AGG + chr1_2:63677459-63677478 None:intergenic 45.0%
TACTCTTCTGTGAAGGTTGG+TGG - chr1_2:63677563-63677582 Msa0084330:CDS 45.0%
TCTCAGCCTGTTCCATGTAT+AGG + chr1_2:63677807-63677826 None:intergenic 45.0%
GAACAGGCTGAGACAGAATA+AGG - chr1_2:63677814-63677833 Msa0084330:five_prime_UTR 45.0%
GAGTTGTTGAGTGGTGCACA+TGG + chr1_2:63674333-63674352 None:intergenic 50.0%
CCAACATTCTTTGGTTGCCC+TGG + chr1_2:63674859-63674878 None:intergenic 50.0%
CCAGGGCAACCAAAGAATGT+TGG - chr1_2:63674856-63674875 Msa0084330:intron 50.0%
! ATTGAAGCACCACTGAACCG+TGG - chr1_2:63674946-63674965 Msa0084330:CDS 50.0%
AAATTGGGTCCACGGTTCAG+TGG + chr1_2:63674958-63674977 None:intergenic 50.0%
CTGTTCCTTGATTCTCCTGC+TGG - chr1_2:63675231-63675250 Msa0084330:intron 50.0%
CAACACCAGCAGGAGAATCA+AGG + chr1_2:63675239-63675258 None:intergenic 50.0%
CTTGATTCTCCTGCTGGTGT+TGG - chr1_2:63675237-63675256 Msa0084330:intron 50.0%
!! CTGTGATTGGTGCTACAGGT+TGG - chr1_2:63675961-63675980 Msa0084330:CDS 50.0%
AGAGTTGCAGTTGCTGAGCA+AGG - chr1_2:63676138-63676157 Msa0084330:intron 50.0%
TCACTCAGAAGCGGCTTGAA+CGG - chr1_2:63676210-63676229 Msa0084330:CDS 50.0%
! CAATCGTCCAGAAGTGCAGA+AGG - chr1_2:63676389-63676408 Msa0084330:intron 50.0%
GCGCTTCATGCCAATGTAAC+AGG - chr1_2:63676411-63676430 Msa0084330:intron 50.0%
CGTACCTGAATACCCATACC+CGG + chr1_2:63676637-63676656 None:intergenic 50.0%
TCCACATGGCGAACTGTGAT+TGG - chr1_2:63677256-63677275 Msa0084330:intron 50.0%
TCCAATCACAGTTCGCCATG+TGG + chr1_2:63677260-63677279 None:intergenic 50.0%
! GAATATCGAGTTGCGGTCAG+TGG + chr1_2:63677396-63677415 None:intergenic 50.0%
!! CTTCTGTGAAGGTTGGTGGA+TGG - chr1_2:63677567-63677586 Msa0084330:CDS 50.0%
!! AATAAGAGGTGCTGGACACG+AGG - chr1_2:63677622-63677641 Msa0084330:exon 50.0%
TTCCATCTTGCATCTCTGCC+TGG - chr1_2:63677854-63677873 Msa0084330:five_prime_UTR 50.0%
TTCCAGGCAGAGATGCAAGA+TGG + chr1_2:63677859-63677878 None:intergenic 50.0%
TGAAACACCGCCACCAACAC+CGG - chr1_2:63675339-63675358 Msa0084330:intron 55.0%
! ACTAACGTGTCCGGTGTTGG+TGG + chr1_2:63675352-63675371 None:intergenic 55.0%
GTCACTAACGTGTCCGGTGT+TGG + chr1_2:63675355-63675374 None:intergenic 55.0%
!! CACGTGTGTCGGTTTCGTGT+CGG - chr1_2:63675423-63675442 Msa0084330:intron 55.0%
! GTTTCGTGTCGGTGGTGCAT+AGG - chr1_2:63675434-63675453 Msa0084330:intron 55.0%
CAAGCCGCTTCTGAGTGATG+CGG + chr1_2:63676208-63676227 None:intergenic 55.0%
! TGAAGCGCCTTCTGCACTTC+TGG + chr1_2:63676399-63676418 None:intergenic 55.0%
! TTAACGCTGGTCTCCGGGTA+TGG - chr1_2:63676621-63676640 Msa0084330:intron 55.0%
! TAACGCTGGTCTCCGGGTAT+GGG - chr1_2:63676622-63676641 Msa0084330:intron 55.0%
GTCTCCGGGTATGGGTATTC+AGG - chr1_2:63676630-63676649 Msa0084330:intron 55.0%
!! AACGTGTCCGGTGTTGGTGG+CGG + chr1_2:63675349-63675368 None:intergenic 60.0%
CGACACGAAACCGACACACG+TGG + chr1_2:63675425-63675444 None:intergenic 60.0%
!! GTGTGTCGGTTTCGTGTCGG+TGG - chr1_2:63675426-63675445 Msa0084330:intron 60.0%
GCTTCGCGGGGCTTATGAAG+AGG + chr1_2:63677650-63677669 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 63674244 63678141 63674244 ID=Msa0084330;Name=Msa0084330
chr1_2 mRNA 63674244 63678141 63674244 ID=Msa0084330-mRNA-1;Parent=Msa0084330;Name=Msa0084330-mRNA-1;_AED=0.30;_eAED=0.30;_QI=504|1|1|1|1|1|9|417|458
chr1_2 exon 63674244 63674807 63674244 ID=Msa0084330-mRNA-1:exon:21366;Parent=Msa0084330-mRNA-1
chr1_2 exon 63674909 63675014 63674909 ID=Msa0084330-mRNA-1:exon:21365;Parent=Msa0084330-mRNA-1
chr1_2 exon 63675734 63675829 63675734 ID=Msa0084330-mRNA-1:exon:21364;Parent=Msa0084330-mRNA-1
chr1_2 exon 63675925 63676052 63675925 ID=Msa0084330-mRNA-1:exon:21363;Parent=Msa0084330-mRNA-1
chr1_2 exon 63676146 63676406 63676146 ID=Msa0084330-mRNA-1:exon:21362;Parent=Msa0084330-mRNA-1
chr1_2 exon 63676511 63676596 63676511 ID=Msa0084330-mRNA-1:exon:21361;Parent=Msa0084330-mRNA-1
chr1_2 exon 63676714 63676806 63676714 ID=Msa0084330-mRNA-1:exon:21360;Parent=Msa0084330-mRNA-1
chr1_2 exon 63677070 63677162 63677070 ID=Msa0084330-mRNA-1:exon:21359;Parent=Msa0084330-mRNA-1
chr1_2 exon 63677271 63678141 63677271 ID=Msa0084330-mRNA-1:exon:21358;Parent=Msa0084330-mRNA-1
chr1_2 five_prime_UTR 63677638 63678141 63677638 ID=Msa0084330-mRNA-1:five_prime_utr;Parent=Msa0084330-mRNA-1
chr1_2 CDS 63677271 63677637 63677271 ID=Msa0084330-mRNA-1:cds;Parent=Msa0084330-mRNA-1
chr1_2 CDS 63677070 63677162 63677070 ID=Msa0084330-mRNA-1:cds;Parent=Msa0084330-mRNA-1
chr1_2 CDS 63676714 63676806 63676714 ID=Msa0084330-mRNA-1:cds;Parent=Msa0084330-mRNA-1
chr1_2 CDS 63676511 63676596 63676511 ID=Msa0084330-mRNA-1:cds;Parent=Msa0084330-mRNA-1
chr1_2 CDS 63676146 63676406 63676146 ID=Msa0084330-mRNA-1:cds;Parent=Msa0084330-mRNA-1
chr1_2 CDS 63675925 63676052 63675925 ID=Msa0084330-mRNA-1:cds;Parent=Msa0084330-mRNA-1
chr1_2 CDS 63675734 63675829 63675734 ID=Msa0084330-mRNA-1:cds;Parent=Msa0084330-mRNA-1
chr1_2 CDS 63674909 63675014 63674909 ID=Msa0084330-mRNA-1:cds;Parent=Msa0084330-mRNA-1
chr1_2 CDS 63674661 63674807 63674661 ID=Msa0084330-mRNA-1:cds;Parent=Msa0084330-mRNA-1
chr1_2 three_prime_UTR 63674244 63674660 63674244 ID=Msa0084330-mRNA-1:three_prime_utr;Parent=Msa0084330-mRNA-1
Gene Sequence

>Msa0084330

ATGGGTTATTCCATATTCTATATTGTTTTGTTTGTGTCCATTTTCTGTGTTGGAATTTCATTGGCTTCTTCTATAGAAGATCAAAAGAGAGATAAGATTAAGCAGTTACCAGGGCAACCAAAGAATGTTGGATTTGAACAGTATTCAGGCTATGTGACTGTGAATGAACAGAATGGAAGGGCACTGTTTTACTGGTTAATTGAAGCACCACTGAACCGTGGACCCAATTTAAGGCCATTAGTTTTATGGCTTAATGGTGGTCCTGGTTGTTCTTCAATTGCTTATGGTGCTTCTGAAGAAATTGGTCCTTTTCGTATTAGGCCTGATGGCAAATCACTTTTCTTGAATCGTTATGCTTGGAACAATTCAAATATACTGTTCCTTGATTCTCCTGCTGGTGTTGGTTTTTCTTATTGTAATAAAACAACAGATCTGTACACATTTGGTGACCAGAAAACAGCTGAGGATGCATATATTTTTCTTGTAAAGTGGTTTGAAAGATTTCCTCAGTATAAGCATAGAGAATTCTACATTGCTGGGGAAAGTTATGCAGGTCATTATGTTCCTCAGTTGGCTCAACTTGTTTACCAGAGAAATAAGGGAATTGACAAACCAGTTATCAATTTTAAGGGATTTATGGTTGGAAATGGTGTGACTGACGATTATCATGACTATGTTGGGACATTTGAGTACTGGTGGACACATGGTTTGATTTCAGATTCTACATATAGGATTCTAAGAATTGCTTGTGACTTTGGATCATCTCAGCATCCATCAGTGCAATGTTTTCAGGCTCTTAGAGTTGCAGTTGCTGAGCAAGGAAACATTGATCCATATAGCATTTATACTCCGCCTTGTAACAATACCGCATCACTCAGAAGCGGCTTGAACGGTCGTTATCCATGGATGTCTAGAGCATATGATCCGTGTACCGAAAGGCATTCTGACGTGTATTTCAATCGTCCAGAAGTGCAGAAGGCGCTTCATGCCAATGTAACAGGGATTCCATATATTTGGAAGACATGCAGTGATATTGTTGGGAATTATTGGACTGATTCCCCATTGTCTATGCTTCCTATATATCATGAACTTATTAACGCTGGTCTCCGGGTATGGGTATTCAGTGGAGACACTGATTCTGTAGTTCCACTGACCGCAACTCGATATTCTATCGATGCCTTGAAGCTACCAACCATCATCAATTGGTATCCTTGGTATGATAGTGGCAAGGTTGGTGGATGGAGTCAAGTTTATAAAGGGCTCACATTGGTAACAATAAGAGGTGCTGGACACGAGGTTCCTCTTCATAAGCCCCGCGAAGCGTTTATTCTTTTTAGATCATTCTTGGAGAACAAGGACATGCCATCATCAAGCTAA
Protein sequence

>Msa0084330

MGYSIFYIVLFVSIFCVGISLASSIEDQKRDKIKQLPGQPKNVGFEQYSGYVTVNEQNGRALFYWLIEAPLNRGPNLRPLVLWLNGGPGCSSIAYGASEEIGPFRIRPDGKSLFLNRYAWNNSNILFLDSPAGVGFSYCNKTTDLYTFGDQKTAEDAYIFLVKWFERFPQYKHREFYIAGESYAGHYVPQLAQLVYQRNKGIDKPVINFKGFMVGNGVTDDYHDYVGTFEYWWTHGLISDSTYRILRIACDFGSSQHPSVQCFQALRVAVAEQGNIDPYSIYTPPCNNTASLRSGLNGRYPWMSRAYDPCTERHSDVYFNRPEVQKALHANVTGIPYIWKTCSDIVGNYWTDSPLSMLPIYHELINAGLRVWVFSGDTDSVVPLTATRYSIDALKLPTIINWYPWYDSGKVGGWSQVYKGLTLVTIRGAGHEVPLHKPREAFILFRSFLENKDMPSSS*