AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0662370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0662370 MtrunA17_Chr1g0199991 97.386 459 12 0 1 459 1 459 0.0 934
Msa0662370 MtrunA17_Chr1g0199971 84.498 458 71 0 1 458 1 458 0.0 818
Msa0662370 MtrunA17_Chr2g0309521 54.466 459 204 3 3 457 6 463 0.0 544
Msa0662370 MtrunA17_Chr2g0309541 54.945 455 196 4 8 457 14 464 0.0 535
Msa0662370 MtrunA17_Chr2g0309511 53.896 462 202 5 3 457 6 463 0.0 531
Msa0662370 MtrunA17_Chr7g0219051 54.881 461 193 7 7 457 10 465 0.0 521
Msa0662370 MtrunA17_Chr2g0318911 53.540 452 207 3 8 457 14 464 0.0 520
Msa0662370 MtrunA17_Chr3g0090331 49.302 430 208 5 28 456 70 490 8.30e-156 451
Msa0662370 MtrunA17_Chr3g0118841 51.765 425 193 6 26 450 72 484 1.21e-154 448
Msa0662370 MtrunA17_Chr4g0038311 48.498 466 225 9 1 456 3 463 3.45e-153 443
Msa0662370 MtrunA17_Chr3g0118881 49.886 437 198 7 31 458 76 500 4.55e-152 441
Msa0662370 MtrunA17_Chr3g0118851 50.952 420 195 6 31 450 76 484 1.11e-148 432
Msa0662370 MtrunA17_Chr1g0153881 49.428 437 199 7 19 450 72 491 1.29e-147 430
Msa0662370 MtrunA17_Chr4g0020241 48.956 431 208 7 28 457 69 488 3.75e-147 429
Msa0662370 MtrunA17_Chr4g0068841 44.328 476 241 7 5 459 8 480 6.24e-141 412
Msa0662370 MtrunA17_Chr4g0047861 46.847 444 220 6 27 458 48 487 8.01e-141 412
Msa0662370 MtrunA17_Chr3g0118831 48.492 431 178 7 29 458 74 461 3.37e-138 405
Msa0662370 MtrunA17_Chr1g0153941 45.295 457 215 6 17 450 62 506 1.28e-136 402
Msa0662370 MtrunA17_Chr2g0281621 44.444 468 243 8 3 458 5 467 2.00e-136 400
Msa0662370 MtrunA17_Chr3g0118861 48.157 434 172 7 28 450 71 462 5.20e-134 394
Msa0662370 MtrunA17_Chr3g0118891 47.619 420 176 7 31 450 76 451 1.16e-132 390
Msa0662370 MtrunA17_Chr4g0068861 44.289 429 211 9 8 412 52 476 3.77e-119 357
Msa0662370 MtrunA17_Chr2g0281641 45.312 384 195 4 87 457 7 388 2.36e-117 349
Msa0662370 MtrunA17_Chr8g0385871 40.086 464 253 11 12 458 9 464 1.64e-116 349
Msa0662370 MtrunA17_Chr2g0304931 42.438 443 231 9 28 458 34 464 3.29e-116 348
Msa0662370 MtrunA17_Chr3g0126031 41.901 463 251 10 6 459 6 459 2.09e-114 344
Msa0662370 MtrunA17_Chr3g0131731 38.478 473 260 12 1 456 3 461 4.53e-113 340
Msa0662370 MtrunA17_Chr5g0431301 40.512 469 259 9 3 459 4 464 1.14e-112 340
Msa0662370 MtrunA17_Chr3g0094811 41.505 465 240 12 5 457 11 455 2.15e-112 338
Msa0662370 MtrunA17_Chr7g0221551 41.002 439 236 11 31 456 29 457 1.25e-110 334
Msa0662370 MtrunA17_Chr3g0126051 39.863 439 252 8 30 459 31 466 1.15e-104 319
Msa0662370 MtrunA17_Chr8g0338461 34.990 483 254 12 19 450 17 490 3.51e-82 262
Msa0662370 MtrunA17_Chr1g0153931 56.000 200 85 2 17 215 60 257 1.48e-76 240
Msa0662370 MtrunA17_Chr7g0249431 34.122 507 258 18 5 455 12 498 1.58e-74 242
Msa0662370 MtrunA17_Chr1g0204191 33.556 450 236 13 26 456 85 490 3.01e-73 239
Msa0662370 MtrunA17_Chr7g0234051 32.727 495 275 13 5 450 5 490 3.65e-73 238
Msa0662370 MtrunA17_Chr1g0165811 47.085 223 107 5 29 251 27 238 2.12e-67 216
Msa0662370 MtrunA17_Chr7g0234041 31.072 457 252 14 6 415 4 444 2.90e-57 196
Msa0662370 MtrunA17_Chr2g0285351 30.417 480 289 17 2 455 9 469 5.06e-55 190
Msa0662370 MtrunA17_Chr4g0009661 31.461 445 265 14 32 452 39 467 1.45e-54 189
Msa0662370 MtrunA17_Chr1g0153921 45.495 222 107 6 230 450 2 210 1.67e-54 181
Msa0662370 MtrunA17_Chr2g0285371 29.828 466 283 16 15 455 23 469 2.65e-54 188
Msa0662370 MtrunA17_Chr4g0009681 30.786 471 290 16 4 452 8 464 2.42e-53 185
Msa0662370 MtrunA17_Chr4g0009651 30.562 445 269 14 32 452 39 467 2.55e-53 186
Msa0662370 MtrunA17_Chr4g0009701 30.995 442 271 13 32 452 39 467 3.38e-53 185
Msa0662370 MtrunA17_Chr3g0133011 30.063 479 295 14 8 455 27 496 1.02e-52 184
Msa0662370 MtrunA17_Chr4g0009711 29.614 466 291 16 8 452 18 467 1.08e-50 178
Msa0662370 MtrunA17_Chr3g0093971 28.017 464 298 12 1 447 2 446 1.31e-50 178
Msa0662370 MtrunA17_Chr4g0009671 29.655 435 260 13 45 452 17 432 8.84e-49 172
Msa0662370 MtrunA17_Chr2g0285381 28.633 461 292 14 13 455 24 465 6.19e-48 171
Msa0662370 MtrunA17_Chr4g0009641 29.358 436 262 11 32 452 39 443 6.90e-48 170
Msa0662370 MtrunA17_Chr2g0327401 27.635 427 261 13 47 453 100 498 4.95e-43 159
Msa0662370 MtrunA17_Chr4g0044051 29.314 423 255 15 49 455 47 441 1.01e-42 157
Msa0662370 MtrunA17_Chr4g0028271 28.505 428 251 13 47 451 97 492 1.44e-42 157
Msa0662370 MtrunA17_Chr1g0171411 28.169 426 252 14 45 447 81 475 2.14e-42 156
Msa0662370 MtrunA17_Chr4g0028261 28.074 431 266 12 38 450 109 513 3.59e-41 154
Msa0662370 MtrunA17_Chr1g0199171 27.208 419 256 13 49 447 92 481 5.80e-40 150
Msa0662370 MtrunA17_Chr4g0028281 27.465 426 262 14 47 453 95 492 1.07e-39 149
Msa0662370 MtrunA17_Chr4g0028241 26.027 438 287 12 32 453 74 490 2.02e-38 145
Msa0662370 MtrunA17_Chr2g0327381 36.937 222 133 4 47 267 94 309 7.51e-38 145
Msa0662370 MtrunA17_Chr5g0447441 27.316 421 266 13 49 455 43 437 1.57e-37 142
Msa0662370 MtrunA17_Chr2g0327371 26.068 468 242 15 50 453 86 513 1.35e-35 138
Msa0662370 MtrunA17_Chr4g0009721 26.752 471 262 17 4 452 7 416 2.30e-34 133
Msa0662370 MtrunA17_Chr1g0165821 37.908 153 87 3 309 456 29 178 3.08e-28 110
Msa0662370 MtrunA17_Chr7g0229631 43.750 128 56 6 8 129 7 124 1.01e-24 99.8
Msa0662370 MtrunA17_Chr7g0229611 46.078 102 41 5 10 105 1 94 3.79e-20 85.1
Msa0662370 MtrunA17_Chr7g0229621 48.515 101 43 5 5 104 1 93 6.67e-20 84.7
Msa0662370 MtrunA17_Chr7g0229651 27.551 196 133 4 215 402 1 195 8.01e-20 87.4
Msa0662370 MtrunA17_Chr2g0279371 32.632 190 104 6 19 203 14 184 1.47e-19 86.3
Msa0662370 MtrunA17_Chr4g0027731 30.968 155 97 4 41 193 27 173 1.68e-14 75.5
Msa0662370 MtrunA17_Chr2g0281631 44.792 96 35 5 3 87 9 97 6.03e-14 68.6
Msa0662370 MtrunA17_Chr7g0229641 56.410 39 17 0 165 203 45 83 9.00e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0662370 AT3G07990.1 76.092 435 104 0 22 456 23 457 0.0 714
Msa0662370 AT2G35780.1 70.481 437 123 3 22 456 19 451 0.0 654
Msa0662370 AT4G30610.1 55.556 468 194 6 1 458 1 464 0.0 540
Msa0662370 AT4G30810.1 58.005 431 176 2 23 450 28 456 0.0 537
Msa0662370 AT2G24010.1 55.632 435 188 4 25 458 23 453 1.32e-180 513
Msa0662370 AT2G24000.1 55.481 447 186 6 22 458 20 463 2.48e-180 512
Msa0662370 AT3G02110.1 54.688 448 183 7 26 458 31 473 8.49e-179 509
Msa0662370 AT2G24010.2 53.556 450 189 5 25 458 23 468 1.57e-175 500
Msa0662370 AT2G35770.1 54.031 459 202 5 3 458 8 460 7.13e-175 498
Msa0662370 AT2G24010.3 51.178 467 183 6 33 458 2 464 1.43e-167 480
Msa0662370 AT5G23210.1 50.110 455 197 8 28 457 47 496 3.88e-152 442
Msa0662370 AT3G63470.1 48.858 438 204 9 28 459 77 500 1.75e-150 438
Msa0662370 AT3G17180.1 48.889 450 211 8 25 458 31 477 1.05e-144 422
Msa0662370 AT1G61130.1 46.996 466 234 8 1 458 2 462 4.75e-143 417
Msa0662370 AT5G08260.1 43.816 477 237 10 5 457 8 477 4.26e-137 403
Msa0662370 AT1G11080.1 46.799 453 220 6 24 458 42 491 1.49e-134 397
Msa0662370 AT1G11080.2 46.799 453 220 6 24 458 42 491 2.13e-134 397
Msa0662370 AT2G24010.4 53.203 359 148 5 33 375 2 356 3.47e-133 388
Msa0662370 AT3G52020.1 46.560 436 218 11 26 456 73 498 7.21e-133 393
Msa0662370 AT5G23210.3 49.268 410 178 8 28 412 47 451 4.04e-131 387
Msa0662370 AT4G15100.1 43.424 479 245 8 3 458 8 483 1.77e-128 381
Msa0662370 AT5G23210.5 48.139 403 182 6 80 457 1 401 4.24e-126 372
Msa0662370 AT5G23210.2 48.354 395 177 6 88 457 8 400 7.45e-125 369
Msa0662370 AT3G17180.2 46.699 409 200 7 25 418 31 436 1.74e-124 369
Msa0662370 AT2G05850.1 41.163 447 246 9 19 458 50 486 2.39e-121 363
Msa0662370 AT3G52000.1 43.349 436 231 11 28 459 59 482 4.31e-118 354
Msa0662370 AT3G52010.1 41.163 447 243 11 21 459 53 487 3.72e-117 352
Msa0662370 AT2G33530.1 43.304 448 233 11 22 456 23 462 4.48e-116 348
Msa0662370 AT2G12480.3 40.000 435 251 6 29 457 28 458 1.13e-111 337
Msa0662370 AT1G43780.1 41.573 445 239 10 30 459 35 473 2.40e-111 337
Msa0662370 AT1G28110.1 41.096 438 237 10 31 456 30 458 3.78e-110 333
Msa0662370 AT1G28110.2 41.096 438 237 10 31 456 30 458 3.78e-110 333
Msa0662370 AT5G42240.1 40.492 447 242 9 29 459 29 467 1.31e-108 330
Msa0662370 AT2G24000.2 41.834 447 184 9 22 458 20 400 8.63e-107 323
Msa0662370 AT2G12480.4 38.479 434 235 7 29 457 28 434 3.32e-104 317
Msa0662370 AT5G23210.4 47.009 351 159 6 88 413 8 356 6.85e-104 314
Msa0662370 AT5G42230.1 38.479 447 251 9 29 459 25 463 5.98e-103 315
Msa0662370 AT5G42230.2 38.479 447 251 9 29 459 25 463 5.98e-103 315
Msa0662370 AT2G12480.1 38.479 434 233 7 29 457 28 432 6.28e-103 314
Msa0662370 AT2G12480.2 38.391 435 233 8 29 457 28 433 4.22e-101 310
Msa0662370 AT4G12910.1 34.747 495 269 11 9 455 9 497 6.39e-82 261
Msa0662370 AT3G25420.1 34.350 492 267 13 14 455 9 494 1.66e-81 260
Msa0662370 AT3G56540.1 61.111 180 66 3 10 189 57 232 5.52e-70 223
Msa0662370 AT4G12910.2 33.554 453 248 10 9 414 9 455 5.56e-68 224
Msa0662370 AT2G22990.1 32.102 433 262 13 33 455 23 433 7.61e-60 202
Msa0662370 AT2G22970.1 32.102 433 260 14 33 455 25 433 6.86e-59 199
Msa0662370 AT2G22920.2 31.409 433 265 13 33 455 25 435 7.50e-59 199
Msa0662370 AT1G73270.2 31.336 434 262 12 33 455 33 441 3.24e-54 187
Msa0662370 AT1G73270.1 31.336 434 262 12 33 455 33 441 3.61e-54 187
Msa0662370 AT2G22990.3 32.108 408 246 12 33 430 23 409 7.84e-54 187
Msa0662370 AT2G22990.4 32.187 407 245 12 33 429 23 408 8.42e-54 186
Msa0662370 AT2G23000.1 28.637 433 279 12 33 455 25 437 4.41e-53 184
Msa0662370 AT2G23000.2 28.637 433 279 12 33 455 25 437 4.41e-53 184
Msa0662370 AT1G73280.1 31.207 439 256 14 33 455 33 441 4.63e-53 184
Msa0662370 AT1G73290.1 30.505 436 261 12 33 455 32 438 4.81e-53 184
Msa0662370 AT2G22970.3 31.340 418 254 13 33 440 25 419 2.08e-52 182
Msa0662370 AT2G23010.1 28.761 452 290 13 15 455 7 437 8.15e-52 181
Msa0662370 AT2G22970.4 31.618 408 246 13 33 430 25 409 1.05e-51 180
Msa0662370 AT3G12203.1 30.193 467 284 15 1 455 1 437 1.46e-51 180
Msa0662370 AT3G10450.1 29.930 431 272 10 33 455 29 437 1.81e-51 180
Msa0662370 AT2G22990.5 30.485 433 260 14 33 455 23 424 3.04e-51 179
Msa0662370 AT2G23010.2 28.540 452 291 13 15 455 7 437 4.39e-51 179
Msa0662370 AT3G12203.3 30.716 433 265 13 33 455 9 416 5.05e-51 178
Msa0662370 AT2G22980.1 30.324 432 255 15 33 450 26 425 1.18e-50 177
Msa0662370 AT1G73300.1 30.137 438 262 14 33 455 33 441 1.86e-50 177
Msa0662370 AT1G73300.2 30.137 438 262 14 33 455 33 441 1.86e-50 177
Msa0662370 AT2G22920.1 31.378 392 239 11 33 415 25 395 1.56e-49 174
Msa0662370 AT1G33540.1 30.269 446 278 13 33 455 29 464 7.07e-49 174
Msa0662370 AT5G36180.1 30.137 438 262 14 33 455 33 441 9.73e-49 173
Msa0662370 AT3G12220.1 30.184 434 263 12 33 453 27 433 1.41e-48 172
Msa0662370 AT3G10450.2 29.540 413 261 10 51 455 4 394 8.76e-48 169
Msa0662370 AT5G22980.1 28.842 423 263 14 45 453 96 494 8.99e-48 172
Msa0662370 AT5G09640.1 27.686 484 292 13 6 455 6 465 1.79e-47 170
Msa0662370 AT1G33540.2 30.140 428 266 13 51 455 4 421 8.08e-47 167
Msa0662370 AT3G12230.1 28.802 434 273 12 33 455 27 435 1.79e-46 167
Msa0662370 AT3G12240.1 28.670 436 271 14 33 455 28 436 3.02e-46 166
Msa0662370 AT3G12203.2 30.446 404 246 13 62 455 1 379 4.49e-46 164
Msa0662370 AT2G22980.4 29.384 422 253 14 33 440 26 416 1.97e-45 164
Msa0662370 AT3G45010.1 27.907 430 274 11 38 453 92 499 2.51e-45 165
Msa0662370 AT1G73290.3 30.633 395 234 10 33 415 32 398 3.03e-45 162
Msa0662370 AT1G73310.1 28.704 432 276 12 33 455 33 441 4.77e-45 163
Msa0662370 AT2G22980.2 29.854 412 244 14 33 430 26 406 5.10e-45 162
Msa0662370 AT3G12220.2 29.567 416 253 12 51 453 15 403 6.00e-45 162
Msa0662370 AT3G10410.1 28.571 434 262 14 38 452 90 494 7.48e-45 164
Msa0662370 AT3G10450.4 29.557 406 257 9 33 430 29 413 1.31e-44 161
Msa0662370 AT2G22990.6 31.065 338 209 10 123 455 1 319 1.09e-41 151
Msa0662370 AT2G22990.2 30.769 338 210 10 123 455 1 319 3.16e-41 149
Msa0662370 AT3G10450.3 29.124 388 246 9 51 430 4 370 8.39e-41 150
Msa0662370 AT2G22920.4 32.686 309 179 10 33 332 25 313 3.25e-40 147
Msa0662370 AT1G73310.2 29.032 403 256 10 33 427 33 413 1.37e-39 148
Msa0662370 AT2G22970.2 32.686 309 178 10 33 332 25 312 3.53e-39 144
Msa0662370 AT1G73280.2 33.829 269 160 7 33 287 33 297 8.11e-39 144
Msa0662370 AT1G73290.2 32.959 267 163 6 33 287 32 294 7.74e-38 140
Msa0662370 AT3G12220.3 28.972 428 224 14 33 453 27 381 3.14e-37 140
Msa0662370 AT1G73280.4 28.732 355 215 11 112 455 12 339 4.37e-35 134
Msa0662370 AT2G22980.3 32.090 268 164 7 33 291 26 284 1.54e-33 129
Msa0662370 AT1G15000.1 28.103 427 264 13 49 459 37 436 1.22e-32 129
Msa0662370 AT1G73280.3 28.739 341 205 11 126 455 1 314 2.02e-32 125
Msa0662370 AT5G36180.5 27.966 354 219 11 112 455 12 339 4.40e-31 122
Msa0662370 AT5G36180.3 27.966 354 219 11 112 455 12 339 4.40e-31 122
Msa0662370 AT2G22980.5 27.760 317 192 11 123 430 1 289 6.49e-27 110
Msa0662370 AT5G36180.2 27.356 329 204 10 112 430 12 315 1.66e-25 106
Msa0662370 AT5G36180.4 27.089 347 207 11 112 437 12 333 1.62e-24 104
Msa0662370 AT5G22960.1 30.693 202 102 5 11 211 10 174 7.84e-24 99.0
Msa0662370 AT1G73310.3 26.646 319 212 7 112 427 12 311 2.88e-23 100
Msa0662370 AT2G22920.3 26.838 272 176 8 189 455 3 256 1.06e-22 97.4
Msa0662370 AT2G27920.1 23.656 465 297 21 9 439 5 445 3.25e-16 81.3
Msa0662370 AT2G22960.1 30.147 136 90 4 321 455 53 184 1.83e-12 66.2

Find 130 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATTTAGTTATCTGTTTC+TGG 0.114317 4_4:-32764 None:intergenic
GTGATATTGTTGGGAATTAT+TGG 0.173667 4_4:+34922 Msa0662370:intron
TAAAACTAATGGCCTTAAAT+TGG 0.173728 4_4:-33331 None:intergenic
TAACAGGGATTCCATATATT+TGG 0.181948 4_4:+34794 Msa0662370:CDS
ACTTGTTTACCAGAGAAATA+AGG 0.220874 4_4:+34181 Msa0662370:CDS
TGAAATTCCAACACAGAAAA+TGG 0.225573 4_4:-33145 None:intergenic
GGTTGTTCTTCAATTGCTTA+TGG 0.253357 4_4:+33372 Msa0662370:CDS
TGTTCAAATCCAACATTCTT+TGG 0.267824 4_4:-33225 None:intergenic
CTGCATGTCTTCCAAATATA+TGG 0.277840 4_4:-34805 None:intergenic
TGAAGCGCCTTCTGCACTTC+TGG 0.279971 4_4:-34763 None:intergenic
GTTGGATTTGAACAGTATTC+AGG 0.287775 4_4:+33234 Msa0662370:CDS
AAACAAAACAATATAGAATA+TGG 0.298366 4_4:-33118 None:intergenic
TGAACCGTGGACCCAATTTA+AGG 0.299323 4_4:+33319 Msa0662370:CDS
CTAAGAATTGCTTGTGACTT+TGG 0.299740 4_4:+34442 Msa0662370:CDS
GCGCTTCATGCCAATGTAAC+AGG 0.306670 4_4:+34778 Msa0662370:CDS
GGGATGTGTATTCTTAGATT+TGG 0.323677 4_4:+33008 Msa0662370:five_prime_UTR
TATGGTGCTTCTGAAGAAAT+TGG 0.325012 4_4:+33390 Msa0662370:CDS
TTCAATTGAAGAACTACTAT+TGG 0.325026 4_4:-36287 None:intergenic
GTCTCCGGGTATGGGTATTC+AGG 0.330012 4_4:+34997 Msa0662370:CDS
TTTCTGTGTTGGAATTTCAT+TGG 0.334125 4_4:+33149 Msa0662370:CDS
AATCCCTTAAAATTGATAAC+TGG 0.337875 4_4:-34215 None:intergenic
TGTCAATTCCCTTATTTCTC+TGG 0.354954 4_4:-34190 None:intergenic
CATAGAGAATTCTACATTGC+TGG 0.368294 4_4:+33998 Msa0662370:CDS
AACTTATTAACGCTGGTCTC+CGG 0.369243 4_4:+34982 Msa0662370:CDS
ATTTATCAAATTTAATTTCC+AGG 0.369979 4_4:-36261 None:intergenic
TTAACGCTGGTCTCCGGGTA+TGG 0.373121 4_4:+34988 Msa0662370:CDS
TATCAAACTTTCCATGTTTG+AGG 0.379926 4_4:-32665 None:intergenic
CTTGTTTACCAGAGAAATAA+GGG 0.385746 4_4:+34182 Msa0662370:CDS
GGATGGAGTCAAGTTTATAA+AGG 0.388461 4_4:+35973 Msa0662370:CDS
GAACAGGCTGAGACAGAATA+AGG 0.395226 4_4:+36203 Msa0662370:three_prime_UTR
GGCGGAGTATAAATGCTATA+TGG 0.395551 4_4:-34538 None:intergenic
CTGTTCCTTGATTCTCCTGC+TGG 0.401847 4_4:+33594 Msa0662370:CDS
CTTGATTCTCCTGCTGGTGT+TGG 0.404698 4_4:+33600 Msa0662370:CDS
AAATTCAAAGCATTCATGAT+TGG 0.405270 4_4:+32780 Msa0662370:five_prime_UTR
TCTCAGCCTGTTCCATGTAT+AGG 0.406780 4_4:-36193 None:intergenic
TGATTTCAGATTCTACATAT+AGG 0.409759 4_4:+34416 Msa0662370:CDS
TACTCTTCTGTGAAGGTTGG+TGG 0.412687 4_4:+35952 Msa0662370:intron
TTCTTGATAGTGATATTGTT+GGG 0.415291 4_4:+34913 Msa0662370:intron
AAAACTAATGGCCTTAAATT+GGG 0.416290 4_4:-33330 None:intergenic
TTAATAAGTTCATGATATAT+AGG 0.416930 4_4:-34969 None:intergenic
AAGTTATTCTTAAGAATGTA+AGG 0.417353 4_4:-36165 None:intergenic
TAAACTTTGCATTTAGCTCC+TGG 0.418833 4_4:+36412 Msa0662370:three_prime_UTR
CCATCATCAATTGGTATCCT+TGG 0.423024 4_4:+35832 Msa0662370:CDS
AAGAAAAGTGATTTGCCATC+AGG 0.425397 4_4:-33429 None:intergenic
CTATCAACTATGTATGCATT+TGG 0.430352 4_4:-32812 None:intergenic
TAATAACATCGTTATTATTG+AGG 0.448081 4_4:-32609 None:intergenic
TTATGGCTTAATGGTGGTCC+TGG 0.448568 4_4:+33351 Msa0662370:CDS
TCAATTCCTATACATGGAAC+AGG 0.448805 4_4:+36187 Msa0662370:three_prime_UTR
GATGATGGTTGGTAGCTTCA+AGG 0.449314 4_4:-35817 None:intergenic
ACAACAGATCTGTACACATT+TGG 0.450890 4_4:+33642 Msa0662370:CDS
TTTCTTGATAGTGATATTGT+TGG 0.454235 4_4:+34912 Msa0662370:intron
ACGATTATCATGACTATGTT+GGG 0.461685 4_4:+34365 Msa0662370:CDS
CCAACATTCTTTGGTTGCCC+TGG 0.461852 4_4:-33216 None:intergenic
CCATTAAGCCATAAAACTAA+TGG 0.465147 4_4:-33342 None:intergenic
ACATTTGGTGACCAGAAAAC+AGG 0.468417 4_4:+33657 Msa0662370:CDS
TTCCATCTTGCATCTCTGCC+TGG 0.468808 4_4:+36243 Msa0662370:three_prime_UTR
GACGATTATCATGACTATGT+TGG 0.470565 4_4:+34364 Msa0662370:CDS
ATATAGGAAGCATAGACAAT+GGG 0.471053 4_4:-34953 None:intergenic
CATATATTTGGAAGACATGC+AGG 0.472543 4_4:+34806 Msa0662370:CDS
CTCACATTGGTAACAATAAG+AGG 0.473526 4_4:+35997 Msa0662370:CDS
ATAGAGAATTCTACATTGCT+GGG 0.479532 4_4:+33999 Msa0662370:CDS
AGCACTAATGATTGACATCC+AGG 0.482811 4_4:-36430 None:intergenic
GCTTCTCATTAGCTTGATGA+TGG 0.483315 4_4:-36099 None:intergenic
AGCTACCAACCATCATCAAT+TGG 0.485261 4_4:+35823 Msa0662370:CDS
AAGTGAGAGACATAAAACTA+TGG 0.485546 4_4:-32907 None:intergenic
ATATGGAATCCCTGTTACAT+TGG 0.488606 4_4:-34788 None:intergenic
GCCTGAAAACATTGCACTGA+TGG 0.488616 4_4:-34478 None:intergenic
AGCACTTCAATAAATTCCAT+TGG 0.490179 4_4:+33046 Msa0662370:five_prime_UTR
ACTTATTAACGCTGGTCTCC+GGG 0.492860 4_4:+34983 Msa0662370:CDS
TAACGCTGGTCTCCGGGTAT+GGG 0.495844 4_4:+34989 Msa0662370:CDS
CAACACCAGCAGGAGAATCA+AGG 0.496627 4_4:-33599 None:intergenic
GATGGAGTCAAGTTTATAAA+GGG 0.496669 4_4:+35974 Msa0662370:CDS
AGGTCATTATGTTCCTCAGT+TGG 0.498140 4_4:+34154 Msa0662370:intron
GATAAGATTAAGCAGTTACC+AGG 0.504649 4_4:+33198 Msa0662370:CDS
TGGTATCCTTGGTATGATAG+TGG 0.505825 4_4:+35843 Msa0662370:CDS
TTGGTAACAATAAGAGGTGC+TGG 0.519871 4_4:+36003 Msa0662370:CDS
GAATATCGAGTTGCGGTCAG+TGG 0.520379 4_4:-35786 None:intergenic
ATGTTGGGACATTTGAGTAC+TGG 0.520555 4_4:+34380 Msa0662370:CDS
TAGATCATTCTTGGAGAACA+AGG 0.525636 4_4:+36071 Msa0662370:CDS
CCAGGGCAACCAAAGAATGT+TGG 0.526279 4_4:+33216 Msa0662370:CDS
CTTCTGTGAAGGTTGGTGGA+TGG 0.542653 4_4:+35956 Msa0662370:intron
CCATCAGGCCTAATACGAAA+AGG 0.545705 4_4:-33414 None:intergenic
ATACACGTCAGAATGCCTTT+CGG 0.549353 4_4:-34731 None:intergenic
TTGGTGCTACAGGTTGGAAA+TGG 0.551086 4_4:+34334 Msa0662370:intron
TCACTCAGAAGCGGCTTGAA+CGG 0.556715 4_4:+34577 Msa0662370:CDS
ATTGCTGGGGAAAGTTATGC+AGG 0.557240 4_4:+34013 Msa0662370:CDS
GGATACCAATTGATGATGGT+TGG 0.557745 4_4:-35828 None:intergenic
ATAACTTCAATTCCTATACA+TGG 0.558875 4_4:+36181 Msa0662370:three_prime_UTR
TCAGAATGCCTTTCGGTACA+CGG 0.563005 4_4:-34724 None:intergenic
CCAAGGATACCAATTGATGA+TGG 0.563122 4_4:-35832 None:intergenic
CTAATGAATGAGAGAACCAA+TGG 0.563685 4_4:-33062 None:intergenic
AGAGTTGCAGTTGCTGAGCA+AGG 0.568738 4_4:+34505 Msa0662370:CDS
CGCTTCATGCCAATGTAACA+GGG 0.570208 4_4:+34779 Msa0662370:CDS
TTCCAGGCAGAGATGCAAGA+TGG 0.576330 4_4:-36245 None:intergenic
ATCTATCTTAGCCTCAAACA+TGG 0.591888 4_4:+32654 Msa0662370:five_prime_UTR
TTTGAGTACTGGTGGACACA+TGG 0.597648 4_4:+34391 Msa0662370:CDS
AGTGATGCGGTATTGTTACA+AGG 0.603572 4_4:-34559 None:intergenic
AAATTGGGTCCACGGTTCAG+TGG 0.604072 4_4:-33315 None:intergenic
CTGTGAATGAACAGAATGGA+AGG 0.605445 4_4:+33265 Msa0662370:CDS
GAGTTGTTGAGTGGTGCACA+TGG 0.606168 4_4:-32692 None:intergenic
TCATATGCTCTAGACATCCA+TGG 0.607409 4_4:-34700 None:intergenic
CAATCGTCCAGAAGTGCAGA+AGG 0.609778 4_4:+34756 Msa0662370:CDS
TATATAGGAAGCATAGACAA+TGG 0.610027 4_4:-34954 None:intergenic
ATGGCCTTAAATTGGGTCCA+CGG 0.610231 4_4:-33323 None:intergenic
GCTTCGCGGGGCTTATGAAG+AGG 0.611657 4_4:-36036 None:intergenic
TATCATGAACTTATTAACGC+TGG 0.615510 4_4:+34975 Msa0662370:CDS
GGAACAAGGCTAAAACTATG+AGG 0.616268 4_4:-32930 None:intergenic
GTGACTGTGAATGAACAGAA+TGG 0.619017 4_4:+33261 Msa0662370:CDS
CATATGATCCGTGTACCGAA+AGG 0.636629 4_4:+34716 Msa0662370:CDS
AAAAGAATAAACGCTTCGCG+GGG 0.640750 4_4:-36048 None:intergenic
CGTACCTGAATACCCATACC+CGG 0.640864 4_4:-35001 None:intergenic
ATAAGATTAAGCAGTTACCA+GGG 0.641370 4_4:+33199 Msa0662370:CDS
AATTCTCTATGCTTATACTG+AGG 0.645681 4_4:-33986 None:intergenic
TTGGGACATTTGAGTACTGG+TGG 0.645881 4_4:+34383 Msa0662370:CDS
ATCGATAGAATATCGAGTTG+CGG 0.654875 4_4:-35793 None:intergenic
AGTTTATAAAGGGCTCACAT+TGG 0.657748 4_4:+35984 Msa0662370:CDS
AATAAGAGGTGCTGGACACG+AGG 0.657878 4_4:+36011 Msa0662370:CDS
TGACTGAGTGAGTTGTTGAG+TGG 0.657980 4_4:-32701 None:intergenic
TCCTTGGTATGATAGTGGCA+AGG 0.660717 4_4:+35848 Msa0662370:CDS
ACCTTGCCACTATCATACCA+AGG 0.669971 4_4:-35849 None:intergenic
TAGAGAATTCTACATTGCTG+GGG 0.672609 4_4:+34000 Msa0662370:CDS
TAAGCAATTGAAGAACAACC+AGG 0.684683 4_4:-33369 None:intergenic
AATACCGCATCACTCAGAAG+CGG 0.685301 4_4:+34568 Msa0662370:CDS
ATTGAAGCACCACTGAACCG+TGG 0.692357 4_4:+33306 Msa0662370:CDS
TATAGGAAGCATAGACAATG+GGG 0.694959 4_4:-34952 None:intergenic
AGTTGTTGAGTGGTGCACAT+GGG 0.696031 4_4:-32691 None:intergenic
CAAGCCGCTTCTGAGTGATG+CGG 0.701434 4_4:-34572 None:intergenic
TAAACAAGTTGAGCCAACTG+AGG 0.719538 4_4:-34167 None:intergenic
TGTGAATGAACAGAATGGAA+GGG 0.721865 4_4:+33266 Msa0662370:CDS
GATGCGGTATTGTTACAAGG+CGG 0.780235 4_4:-34556 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTGTTTTATAGAATAA+TGG + chr4_4:33089-33108 Msa0662370:exon 10.0%
!!! TTTTTGTTTTATAGAATAAT+GGG + chr4_4:33090-33109 Msa0662370:exon 10.0%
!! AAACAAAACAATATAGAATA+TGG - chr4_4:33121-33140 None:intergenic 15.0%
!!! TTTATGCCTATTTATTATTT+TGG + chr4_4:33551-33570 Msa0662370:intron 15.0%
!!! TATCAGAATTTTGATTTAAA+AGG + chr4_4:34876-34895 Msa0662370:intron 15.0%
!! TTAATAAGTTCATGATATAT+AGG - chr4_4:34972-34991 None:intergenic 15.0%
!!! ACTTTTTTGCTTATATTTAT+CGG + chr4_4:35051-35070 Msa0662370:intron 15.0%
!!! ATTTATCAAATTTAATTTCC+AGG - chr4_4:36264-36283 None:intergenic 15.0%
!! TAATAACATCGTTATTATTG+AGG - chr4_4:32612-32631 None:intergenic 20.0%
!!! AGTTTTAGATCTTTTAGTAT+TGG + chr4_4:32987-33006 Msa0662370:five_prime_UTR 20.0%
!!! GTTTTAGATCTTTTAGTATT+GGG + chr4_4:32988-33007 Msa0662370:five_prime_UTR 20.0%
!! CAACATCCAAAATAATAAAT+AGG - chr4_4:33560-33579 None:intergenic 20.0%
!!! CATATATTTTTCTTGTAAAG+TGG + chr4_4:33951-33970 Msa0662370:CDS 20.0%
!!! TATATATCAGTTTTCTACTT+TGG + chr4_4:34051-34070 Msa0662370:intron 20.0%
!! ATTCAATTGATAAGTAAGTA+TGG - chr4_4:34079-34098 None:intergenic 20.0%
!!! TGAAAAATTGAATGATTTTC+AGG + chr4_4:34134-34153 Msa0662370:intron 20.0%
!!! AAACCAGTTATCAATTTTAA+GGG + chr4_4:34212-34231 Msa0662370:CDS 20.0%
!!! TATCAATTTTAAGGGATTTA+TGG + chr4_4:34220-34239 Msa0662370:CDS 20.0%
!! ATAAACACAACAAAAGTAAT+GGG - chr4_4:35281-35300 None:intergenic 20.0%
!! AATAAACACAACAAAAGTAA+TGG - chr4_4:35282-35301 None:intergenic 20.0%
!!! AAGGAACAAAGATATTTTTT+TGG - chr4_4:35353-35372 None:intergenic 20.0%
!! ATATTATGATGTGATTTGAT+TGG - chr4_4:35589-35608 None:intergenic 20.0%
!!! TCTTCTTAACTTTCATATTT+TGG + chr4_4:35735-35754 Msa0662370:intron 20.0%
!!! TGTTCAAGTTTATACTAATT+TGG + chr4_4:35898-35917 Msa0662370:intron 20.0%
!!! TATTCTTTTTAGATCATTCT+TGG + chr4_4:36062-36081 Msa0662370:CDS 20.0%
!! AAGTTATTCTTAAGAATGTA+AGG - chr4_4:36168-36187 None:intergenic 20.0%
! TGAATTTAGTTATCTGTTTC+TGG - chr4_4:32767-32786 None:intergenic 25.0%
!! AAATTCAAAGCATTCATGAT+TGG + chr4_4:32780-32799 Msa0662370:five_prime_UTR 25.0%
!! AAAACTAATGGCCTTAAATT+GGG - chr4_4:33333-33352 None:intergenic 25.0%
!! TAAAACTAATGGCCTTAAAT+TGG - chr4_4:33334-33353 None:intergenic 25.0%
!!! ATTTAAGGCCATTAGTTTTA+TGG + chr4_4:33334-33353 Msa0662370:CDS 25.0%
!! CAAACCAGTTATCAATTTTA+AGG + chr4_4:34211-34230 Msa0662370:CDS 25.0%
!! AATCCCTTAAAATTGATAAC+TGG - chr4_4:34218-34237 None:intergenic 25.0%
! GTTATATGATTATCATAAGG+TGG - chr4_4:34295-34314 None:intergenic 25.0%
! CTCGTTATATGATTATCATA+AGG - chr4_4:34298-34317 None:intergenic 25.0%
! TGATTTCAGATTCTACATAT+AGG + chr4_4:34416-34435 Msa0662370:CDS 25.0%
! TCAATAAATCGATAGCATAT+AGG - chr4_4:34615-34634 None:intergenic 25.0%
!!! TATGCTATCGATTTATTGAA+TGG + chr4_4:34616-34635 Msa0662370:intron 25.0%
! AACAAATTAAACACAGTTGA+TGG - chr4_4:34659-34678 None:intergenic 25.0%
!!! TTTCTTGATAGTGATATTGT+TGG + chr4_4:34912-34931 Msa0662370:intron 25.0%
!!! TTCTTGATAGTGATATTGTT+GGG + chr4_4:34913-34932 Msa0662370:intron 25.0%
! ATGTATTTGGCATATAGTTA+AGG - chr4_4:35029-35048 None:intergenic 25.0%
!! AGCAAAAAAGTACATGTATT+TGG - chr4_4:35042-35061 None:intergenic 25.0%
!! TAGTATGTGATATCAACTTT+TGG + chr4_4:35195-35214 Msa0662370:intron 25.0%
! TAGAGAAATATGAAATGGAT+GGG - chr4_4:35411-35430 None:intergenic 25.0%
! TTAGAGAAATATGAAATGGA+TGG - chr4_4:35412-35431 None:intergenic 25.0%
! GCATTTAGAGAAATATGAAA+TGG - chr4_4:35416-35435 None:intergenic 25.0%
! CAATTTGTACTAGTAACATT+CGG + chr4_4:35480-35499 Msa0662370:intron 25.0%
! AATTTGTACTAGTAACATTC+GGG + chr4_4:35481-35500 Msa0662370:intron 25.0%
! TCATTGATCTATACAGAATA+TGG - chr4_4:35529-35548 None:intergenic 25.0%
! TCTGTATAGATCAATGATTA+TGG + chr4_4:35532-35551 Msa0662370:intron 25.0%
! TATCTCAATAAATATCCACA+TGG + chr4_4:35634-35653 Msa0662370:intron 25.0%
! CTTATTCTGTAATCAAGAAT+TGG + chr4_4:35709-35728 Msa0662370:intron 25.0%
!! TAAATTTTACTCTTCTGTGA+AGG + chr4_4:35945-35964 Msa0662370:intron 25.0%
!!! AAGAATGTAAGGTACTTTTT+TGG - chr4_4:36157-36176 None:intergenic 25.0%
! ATAACTTCAATTCCTATACA+TGG + chr4_4:36181-36200 Msa0662370:three_prime_UTR 25.0%
! TTCAATTGAAGAACTACTAT+TGG - chr4_4:36290-36309 None:intergenic 25.0%
TATCAAACTTTCCATGTTTG+AGG - chr4_4:32668-32687 None:intergenic 30.0%
CTATCAACTATGTATGCATT+TGG - chr4_4:32815-32834 None:intergenic 30.0%
AAGTGAGAGACATAAAACTA+TGG - chr4_4:32910-32929 None:intergenic 30.0%
ATAGAGAAAAACAAGGAACA+AGG - chr4_4:32947-32966 None:intergenic 30.0%
AGCACTTCAATAAATTCCAT+TGG + chr4_4:33046-33065 Msa0662370:five_prime_UTR 30.0%
TGAAATTCCAACACAGAAAA+TGG - chr4_4:33148-33167 None:intergenic 30.0%
TTTCTGTGTTGGAATTTCAT+TGG + chr4_4:33149-33168 Msa0662370:CDS 30.0%
ATAAGATTAAGCAGTTACCA+GGG + chr4_4:33199-33218 Msa0662370:CDS 30.0%
TGTTCAAATCCAACATTCTT+TGG - chr4_4:33228-33247 None:intergenic 30.0%
CCATTAAGCCATAAAACTAA+TGG - chr4_4:33345-33364 None:intergenic 30.0%
!!! CCATTAGTTTTATGGCTTAA+TGG + chr4_4:33342-33361 Msa0662370:CDS 30.0%
!!! TTAGTTTTATGGCTTAATGG+TGG + chr4_4:33345-33364 Msa0662370:CDS 30.0%
! AAATTGGTCCTTTTCGTATT+AGG + chr4_4:33406-33425 Msa0662370:CDS 30.0%
! TTTTCTTGAATCGTTATGCT+TGG + chr4_4:33445-33464 Msa0662370:CDS 30.0%
!!! TATTATTTTGGATGTTGCAG+TGG + chr4_4:33563-33582 Msa0662370:intron 30.0%
AATTCTCTATGCTTATACTG+AGG - chr4_4:33989-34008 None:intergenic 30.0%
ATAGAGAATTCTACATTGCT+GGG + chr4_4:33999-34018 Msa0662370:CDS 30.0%
ACTTGTTTACCAGAGAAATA+AGG + chr4_4:34181-34200 Msa0662370:CDS 30.0%
CTTGTTTACCAGAGAAATAA+GGG + chr4_4:34182-34201 Msa0662370:CDS 30.0%
GGATTTATGGTAAGATTGTT+AGG + chr4_4:34233-34252 Msa0662370:intron 30.0%
GAGTTTGATTATTCTGTGAT+TGG + chr4_4:34315-34334 Msa0662370:intron 30.0%
ACGATTATCATGACTATGTT+GGG + chr4_4:34365-34384 Msa0662370:CDS 30.0%
TCTTATAAAACAAACAGCCA+TGG + chr4_4:34683-34702 Msa0662370:intron 30.0%
TAACAGGGATTCCATATATT+TGG + chr4_4:34794-34813 Msa0662370:CDS 30.0%
GTGATATTGTTGGGAATTAT+TGG + chr4_4:34922-34941 Msa0662370:intron 30.0%
! ATATAGGAAGCATAGACAAT+GGG - chr4_4:34956-34975 None:intergenic 30.0%
! TATATAGGAAGCATAGACAA+TGG - chr4_4:34957-34976 None:intergenic 30.0%
TATCATGAACTTATTAACGC+TGG + chr4_4:34975-34994 Msa0662370:CDS 30.0%
TTGATATCACATACTATCCA+TGG - chr4_4:35191-35210 None:intergenic 30.0%
AAGTGACACTCAAATGATTA+AGG - chr4_4:35372-35391 None:intergenic 30.0%
AATGCTCTTTGACATTATTG+AGG + chr4_4:35431-35450 Msa0662370:intron 30.0%
ACAGAATATGGAGTAATGAA+AGG - chr4_4:35517-35536 None:intergenic 30.0%
GATGGAGTCAAGTTTATAAA+GGG + chr4_4:35974-35993 Msa0662370:CDS 30.0%
TAAAAAGAATAAACGCTTCG+CGG - chr4_4:36053-36072 None:intergenic 30.0%
ATCTATCTTAGCCTCAAACA+TGG + chr4_4:32654-32673 Msa0662370:five_prime_UTR 35.0%
!!! TTCCTTGTTTTTCTCTATGC+AGG + chr4_4:32949-32968 Msa0662370:five_prime_UTR 35.0%
GGGATGTGTATTCTTAGATT+TGG + chr4_4:33008-33027 Msa0662370:five_prime_UTR 35.0%
CTAATGAATGAGAGAACCAA+TGG - chr4_4:33065-33084 None:intergenic 35.0%
! TTTGTGTCCATTTTCTGTGT+TGG + chr4_4:33138-33157 Msa0662370:CDS 35.0%
GATAAGATTAAGCAGTTACC+AGG + chr4_4:33198-33217 Msa0662370:CDS 35.0%
GTTGGATTTGAACAGTATTC+AGG + chr4_4:33234-33253 Msa0662370:CDS 35.0%
TGTGAATGAACAGAATGGAA+GGG + chr4_4:33266-33285 Msa0662370:CDS 35.0%
TAAGCAATTGAAGAACAACC+AGG - chr4_4:33372-33391 None:intergenic 35.0%
GGTTGTTCTTCAATTGCTTA+TGG + chr4_4:33372-33391 Msa0662370:CDS 35.0%
! TATGGTGCTTCTGAAGAAAT+TGG + chr4_4:33390-33409 Msa0662370:CDS 35.0%
! AAGAAAAGTGATTTGCCATC+AGG - chr4_4:33432-33451 None:intergenic 35.0%
CAAAGACTGAATCTCAGAAT+CGG - chr4_4:33484-33503 None:intergenic 35.0%
ACAACAGATCTGTACACATT+TGG + chr4_4:33642-33661 Msa0662370:CDS 35.0%
! TACATTACCTACCTGTTTTC+TGG - chr4_4:33671-33690 None:intergenic 35.0%
TGTAATGATCATGCTCATGA+AGG + chr4_4:33822-33841 Msa0662370:intron 35.0%
TTGCTAAAGCAAAACAAAGC+TGG - chr4_4:33862-33881 None:intergenic 35.0%
CATAGAGAATTCTACATTGC+TGG + chr4_4:33998-34017 Msa0662370:CDS 35.0%
TAGAGAATTCTACATTGCTG+GGG + chr4_4:34000-34019 Msa0662370:CDS 35.0%
! TGTCAATTCCCTTATTTCTC+TGG - chr4_4:34193-34212 None:intergenic 35.0%
GACGATTATCATGACTATGT+TGG + chr4_4:34364-34383 Msa0662370:CDS 35.0%
CTAAGAATTGCTTGTGACTT+TGG + chr4_4:34442-34461 Msa0662370:CDS 35.0%
ATATGGAATCCCTGTTACAT+TGG - chr4_4:34791-34810 None:intergenic 35.0%
CTGCATGTCTTCCAAATATA+TGG - chr4_4:34808-34827 None:intergenic 35.0%
CATATATTTGGAAGACATGC+AGG + chr4_4:34806-34825 Msa0662370:CDS 35.0%
ATGTTGAACACGTTCTATTG+AGG + chr4_4:34846-34865 Msa0662370:intron 35.0%
! TATAGGAAGCATAGACAATG+GGG - chr4_4:34955-34974 None:intergenic 35.0%
TTGACATCTGAATACATCCA+TGG + chr4_4:35171-35190 Msa0662370:intron 35.0%
! ATCGATAGAATATCGAGTTG+CGG - chr4_4:35796-35815 None:intergenic 35.0%
! TTTTACTCTTCTGTGAAGGT+TGG + chr4_4:35949-35968 Msa0662370:intron 35.0%
GGATGGAGTCAAGTTTATAA+AGG + chr4_4:35973-35992 Msa0662370:CDS 35.0%
AGTTTATAAAGGGCTCACAT+TGG + chr4_4:35984-36003 Msa0662370:CDS 35.0%
! CTCACATTGGTAACAATAAG+AGG + chr4_4:35997-36016 Msa0662370:CDS 35.0%
AAAAAGAATAAACGCTTCGC+GGG - chr4_4:36052-36071 None:intergenic 35.0%
TAGATCATTCTTGGAGAACA+AGG + chr4_4:36071-36090 Msa0662370:CDS 35.0%
TCAATTCCTATACATGGAAC+AGG + chr4_4:36187-36206 Msa0662370:three_prime_UTR 35.0%
! TAAACTTTGCATTTAGCTCC+TGG + chr4_4:36412-36431 Msa0662370:three_prime_UTR 35.0%
GGAACAAGGCTAAAACTATG+AGG - chr4_4:32933-32952 None:intergenic 40.0%
TGCCTGCATAGAGAAAAACA+AGG - chr4_4:32954-32973 None:intergenic 40.0%
GTGACTGTGAATGAACAGAA+TGG + chr4_4:33261-33280 Msa0662370:CDS 40.0%
CTGTGAATGAACAGAATGGA+AGG + chr4_4:33265-33284 Msa0662370:CDS 40.0%
TAAGAAAAACCAACACCAGC+AGG - chr4_4:33612-33631 None:intergenic 40.0%
! ACATTTGGTGACCAGAAAAC+AGG + chr4_4:33657-33676 Msa0662370:CDS 40.0%
CGTTAGTGACACATTGACAT+TGG + chr4_4:33728-33747 Msa0662370:intron 40.0%
! TTGAGTTTTACCACGTGTGT+CGG + chr4_4:33775-33794 Msa0662370:intron 40.0%
GCTCATGAAGGATTTACTCA+TGG + chr4_4:33834-33853 Msa0662370:intron 40.0%
AGGTCATTATGTTCCTCAGT+TGG + chr4_4:34154-34173 Msa0662370:intron 40.0%
! TAAACAAGTTGAGCCAACTG+AGG - chr4_4:34170-34189 None:intergenic 40.0%
!! TATTCTGTGATTGGTGCTAC+AGG + chr4_4:34324-34343 Msa0662370:intron 40.0%
ATGTTGGGACATTTGAGTAC+TGG + chr4_4:34380-34399 Msa0662370:CDS 40.0%
! TCCATCAGTGCAATGTTTTC+AGG + chr4_4:34477-34496 Msa0662370:CDS 40.0%
GGCGGAGTATAAATGCTATA+TGG - chr4_4:34541-34560 None:intergenic 40.0%
AGTGATGCGGTATTGTTACA+AGG - chr4_4:34562-34581 None:intergenic 40.0%
! TCATATGCTCTAGACATCCA+TGG - chr4_4:34703-34722 None:intergenic 40.0%
ATACACGTCAGAATGCCTTT+CGG - chr4_4:34734-34753 None:intergenic 40.0%
! AACTTATTAACGCTGGTCTC+CGG + chr4_4:34982-35001 Msa0662370:CDS 40.0%
TTGACATTATTGAGGCTCAC+TGG + chr4_4:35439-35458 Msa0662370:intron 40.0%
!!! CTTTCATATTTTGGCTGCAG+TGG + chr4_4:35744-35763 Msa0662370:intron 40.0%
AGCTACCAACCATCATCAAT+TGG + chr4_4:35823-35842 Msa0662370:CDS 40.0%
!! GGATACCAATTGATGATGGT+TGG - chr4_4:35831-35850 None:intergenic 40.0%
CCAAGGATACCAATTGATGA+TGG - chr4_4:35835-35854 None:intergenic 40.0%
CCATCATCAATTGGTATCCT+TGG + chr4_4:35832-35851 Msa0662370:CDS 40.0%
TGGTATCCTTGGTATGATAG+TGG + chr4_4:35843-35862 Msa0662370:CDS 40.0%
! TTGGTAACAATAAGAGGTGC+TGG + chr4_4:36003-36022 Msa0662370:CDS 40.0%
AAAAGAATAAACGCTTCGCG+GGG - chr4_4:36051-36070 None:intergenic 40.0%
! GCTTCTCATTAGCTTGATGA+TGG - chr4_4:36102-36121 None:intergenic 40.0%
AGCACTAATGATTGACATCC+AGG - chr4_4:36433-36452 None:intergenic 40.0%
AGTTGTTGAGTGGTGCACAT+GGG - chr4_4:32694-32713 None:intergenic 45.0%
TGACTGAGTGAGTTGTTGAG+TGG - chr4_4:32704-32723 None:intergenic 45.0%
!!! ATGGAAGGGCACTGTTTTAC+TGG + chr4_4:33280-33299 Msa0662370:CDS 45.0%
TGAACCGTGGACCCAATTTA+AGG + chr4_4:33319-33338 Msa0662370:CDS 45.0%
! ATGGCCTTAAATTGGGTCCA+CGG - chr4_4:33326-33345 None:intergenic 45.0%
TTATGGCTTAATGGTGGTCC+TGG + chr4_4:33351-33370 Msa0662370:CDS 45.0%
CCATCAGGCCTAATACGAAA+AGG - chr4_4:33417-33436 None:intergenic 45.0%
! CCTTTTCGTATTAGGCCTGA+TGG + chr4_4:33414-33433 Msa0662370:CDS 45.0%
! TTGGTGACCAGAAAACAGGT+AGG + chr4_4:33661-33680 Msa0662370:intron 45.0%
! CAATGTGTCACTAACGTGTC+CGG - chr4_4:33724-33743 None:intergenic 45.0%
GTTTCTTGACTTTGCAGCTG+AGG + chr4_4:33925-33944 Msa0662370:intron 45.0%
ATTGCTGGGGAAAGTTATGC+AGG + chr4_4:34013-34032 Msa0662370:CDS 45.0%
!! TTGGTGCTACAGGTTGGAAA+TGG + chr4_4:34334-34353 Msa0662370:intron 45.0%
!! TTGGGACATTTGAGTACTGG+TGG + chr4_4:34383-34402 Msa0662370:CDS 45.0%
!! TTTGAGTACTGGTGGACACA+TGG + chr4_4:34391-34410 Msa0662370:CDS 45.0%
GCCTGAAAACATTGCACTGA+TGG - chr4_4:34481-34500 None:intergenic 45.0%
GATGCGGTATTGTTACAAGG+CGG - chr4_4:34559-34578 None:intergenic 45.0%
AATACCGCATCACTCAGAAG+CGG + chr4_4:34568-34587 Msa0662370:CDS 45.0%
CATATGATCCGTGTACCGAA+AGG + chr4_4:34716-34735 Msa0662370:CDS 45.0%
TCAGAATGCCTTTCGGTACA+CGG - chr4_4:34727-34746 None:intergenic 45.0%
CGCTTCATGCCAATGTAACA+GGG + chr4_4:34779-34798 Msa0662370:CDS 45.0%
! ACTTATTAACGCTGGTCTCC+GGG + chr4_4:34983-35002 Msa0662370:CDS 45.0%
!! GATGATGGTTGGTAGCTTCA+AGG - chr4_4:35820-35839 None:intergenic 45.0%
!! TCCTTGGTATGATAGTGGCA+AGG + chr4_4:35848-35867 Msa0662370:CDS 45.0%
ACCTTGCCACTATCATACCA+AGG - chr4_4:35852-35871 None:intergenic 45.0%
TACTCTTCTGTGAAGGTTGG+TGG + chr4_4:35952-35971 Msa0662370:intron 45.0%
TCTCAGCCTGTTCCATGTAT+AGG - chr4_4:36196-36215 None:intergenic 45.0%
GAACAGGCTGAGACAGAATA+AGG + chr4_4:36203-36222 Msa0662370:three_prime_UTR 45.0%
GAGTTGTTGAGTGGTGCACA+TGG - chr4_4:32695-32714 None:intergenic 50.0%
CCAACATTCTTTGGTTGCCC+TGG - chr4_4:33219-33238 None:intergenic 50.0%
CCAGGGCAACCAAAGAATGT+TGG + chr4_4:33216-33235 Msa0662370:CDS 50.0%
! ATTGAAGCACCACTGAACCG+TGG + chr4_4:33306-33325 Msa0662370:CDS 50.0%
AAATTGGGTCCACGGTTCAG+TGG - chr4_4:33318-33337 None:intergenic 50.0%
CTGTTCCTTGATTCTCCTGC+TGG + chr4_4:33594-33613 Msa0662370:CDS 50.0%
CAACACCAGCAGGAGAATCA+AGG - chr4_4:33602-33621 None:intergenic 50.0%
CTTGATTCTCCTGCTGGTGT+TGG + chr4_4:33600-33619 Msa0662370:CDS 50.0%
!! CTGTGATTGGTGCTACAGGT+TGG + chr4_4:34328-34347 Msa0662370:intron 50.0%
AGAGTTGCAGTTGCTGAGCA+AGG + chr4_4:34505-34524 Msa0662370:CDS 50.0%
TCACTCAGAAGCGGCTTGAA+CGG + chr4_4:34577-34596 Msa0662370:CDS 50.0%
! CAATCGTCCAGAAGTGCAGA+AGG + chr4_4:34756-34775 Msa0662370:CDS 50.0%
GCGCTTCATGCCAATGTAAC+AGG + chr4_4:34778-34797 Msa0662370:CDS 50.0%
CGTACCTGAATACCCATACC+CGG - chr4_4:35004-35023 None:intergenic 50.0%
TCCACATGGCGAACTGTGAT+TGG + chr4_4:35648-35667 Msa0662370:intron 50.0%
TCCAATCACAGTTCGCCATG+TGG - chr4_4:35652-35671 None:intergenic 50.0%
! GAATATCGAGTTGCGGTCAG+TGG - chr4_4:35789-35808 None:intergenic 50.0%
!! CTTCTGTGAAGGTTGGTGGA+TGG + chr4_4:35956-35975 Msa0662370:intron 50.0%
!! AATAAGAGGTGCTGGACACG+AGG + chr4_4:36011-36030 Msa0662370:CDS 50.0%
TTCCATCTTGCATCTCTGCC+TGG + chr4_4:36243-36262 Msa0662370:three_prime_UTR 50.0%
TTCCAGGCAGAGATGCAAGA+TGG - chr4_4:36248-36267 None:intergenic 50.0%
TGAAACACCGCCACCAACAC+CGG + chr4_4:33702-33721 Msa0662370:intron 55.0%
! ACTAACGTGTCCGGTGTTGG+TGG - chr4_4:33715-33734 None:intergenic 55.0%
GTCACTAACGTGTCCGGTGT+TGG - chr4_4:33718-33737 None:intergenic 55.0%
!! CACGTGTGTCGGTTTCGTGT+CGG + chr4_4:33786-33805 Msa0662370:intron 55.0%
! GTTTCGTGTCGGTGGTGCAT+AGG + chr4_4:33797-33816 Msa0662370:intron 55.0%
CAAGCCGCTTCTGAGTGATG+CGG - chr4_4:34575-34594 None:intergenic 55.0%
! TGAAGCGCCTTCTGCACTTC+TGG - chr4_4:34766-34785 None:intergenic 55.0%
! TTAACGCTGGTCTCCGGGTA+TGG + chr4_4:34988-35007 Msa0662370:CDS 55.0%
! TAACGCTGGTCTCCGGGTAT+GGG + chr4_4:34989-35008 Msa0662370:CDS 55.0%
GTCTCCGGGTATGGGTATTC+AGG + chr4_4:34997-35016 Msa0662370:CDS 55.0%
!! AACGTGTCCGGTGTTGGTGG+CGG - chr4_4:33712-33731 None:intergenic 60.0%
CGACACGAAACCGACACACG+TGG - chr4_4:33788-33807 None:intergenic 60.0%
!! GTGTGTCGGTTTCGTGTCGG+TGG + chr4_4:33789-33808 Msa0662370:intron 60.0%
GCTTCGCGGGGCTTATGAAG+AGG - chr4_4:36039-36058 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 32602 36530 32602 ID=Msa0662370;Name=Msa0662370
chr4_4 mRNA 32602 36530 32602 ID=Msa0662370-mRNA-1;Parent=Msa0662370;Name=Msa0662370-mRNA-1;_AED=0.13;_eAED=0.13;_QI=506|1|1|1|1|1|9|417|459
chr4_4 exon 32602 33474 32602 ID=Msa0662370-mRNA-1:exon:2;Parent=Msa0662370-mRNA-1
chr4_4 exon 33583 33678 33583 ID=Msa0662370-mRNA-1:exon:3;Parent=Msa0662370-mRNA-1
chr4_4 exon 33942 34034 33942 ID=Msa0662370-mRNA-1:exon:4;Parent=Msa0662370-mRNA-1
chr4_4 exon 34156 34241 34156 ID=Msa0662370-mRNA-1:exon:5;Parent=Msa0662370-mRNA-1
chr4_4 exon 34346 34606 34346 ID=Msa0662370-mRNA-1:exon:6;Parent=Msa0662370-mRNA-1
chr4_4 exon 34700 34827 34700 ID=Msa0662370-mRNA-1:exon:7;Parent=Msa0662370-mRNA-1
chr4_4 exon 34923 35018 34923 ID=Msa0662370-mRNA-1:exon:8;Parent=Msa0662370-mRNA-1
chr4_4 exon 35764 35869 35764 ID=Msa0662370-mRNA-1:exon:9;Parent=Msa0662370-mRNA-1
chr4_4 exon 35967 36530 35967 ID=Msa0662370-mRNA-1:exon:10;Parent=Msa0662370-mRNA-1
chr4_4 five_prime_UTR 32602 33107 32602 ID=Msa0662370-mRNA-1:five_prime_utr;Parent=Msa0662370-mRNA-1
chr4_4 CDS 33108 33474 33108 ID=Msa0662370-mRNA-1:cds;Parent=Msa0662370-mRNA-1
chr4_4 CDS 33583 33678 33583 ID=Msa0662370-mRNA-1:cds;Parent=Msa0662370-mRNA-1
chr4_4 CDS 33942 34034 33942 ID=Msa0662370-mRNA-1:cds;Parent=Msa0662370-mRNA-1
chr4_4 CDS 34156 34241 34156 ID=Msa0662370-mRNA-1:cds;Parent=Msa0662370-mRNA-1
chr4_4 CDS 34346 34606 34346 ID=Msa0662370-mRNA-1:cds;Parent=Msa0662370-mRNA-1
chr4_4 CDS 34700 34827 34700 ID=Msa0662370-mRNA-1:cds;Parent=Msa0662370-mRNA-1
chr4_4 CDS 34923 35018 34923 ID=Msa0662370-mRNA-1:cds;Parent=Msa0662370-mRNA-1
chr4_4 CDS 35764 35869 35764 ID=Msa0662370-mRNA-1:cds;Parent=Msa0662370-mRNA-1
chr4_4 CDS 35967 36113 35967 ID=Msa0662370-mRNA-1:cds;Parent=Msa0662370-mRNA-1
chr4_4 three_prime_UTR 36114 36530 36114 ID=Msa0662370-mRNA-1:three_prime_utr;Parent=Msa0662370-mRNA-1
Gene Sequence

>Msa0662370

ATGGGTTATTCCATATTCTATATTGTTTTGTTTGTGTCCATTTTCTGTGTTGGAATTTCATTGGCTTCTTCTATAGAAGATCAAAAGAGAGATAAGATTAAGCAGTTACCAGGGCAACCAAAGAATGTTGGATTTGAACAGTATTCAGGCTATGTGACTGTGAATGAACAGAATGGAAGGGCACTGTTTTACTGGTTAATTGAAGCACCACTGAACCGTGGACCCAATTTAAGGCCATTAGTTTTATGGCTTAATGGTGGTCCTGGTTGTTCTTCAATTGCTTATGGTGCTTCTGAAGAAATTGGTCCTTTTCGTATTAGGCCTGATGGCAAATCACTTTTCTTGAATCGTTATGCTTGGAACAATTTGGCAAATATACTGTTCCTTGATTCTCCTGCTGGTGTTGGTTTTTCTTATTGTACTAAAACAACAGATCTGTACACATTTGGTGACCAGAAAACAGCTGAGGATGCATATATTTTTCTTGTAAAGTGGTTTGAAAGATTTCCTCAGTATAAGCATAGAGAATTCTACATTGCTGGGGAAAGTTATGCAGGTCATTATGTTCCTCAGTTGGCTCAACTTGTTTACCAGAGAAATAAGGGAATTGACAAACCAGTTATCAATTTTAAGGGATTTATGGTTGGAAATGGTGTGACTGACGATTATCATGACTATGTTGGGACATTTGAGTACTGGTGGACACATGGTTTGATTTCAGATTCTACATATAGGATTCTAAGAATTGCTTGTGACTTTGGATCATCTCAGCATCCATCAGTGCAATGTTTTCAGGCTCTTAGAGTTGCAGTTGCTGAGCAAGGAAACATTGATCCATATAGCATTTATACTCCGCCTTGTAACAATACCGCATCACTCAGAAGCGGCTTGAACGGTCGTTATCCATGGATGTCTAGAGCATATGATCCGTGTACCGAAAGGCATTCTGACGTGTATTTCAATCGTCCAGAAGTGCAGAAGGCGCTTCATGCCAATGTAACAGGGATTCCATATATTTGGAAGACATGCAGTGATATTGTTGGGAATTATTGGACTGATTCCCCATTGTCTATGCTTCCTATATATCATGAACTTATTAACGCTGGTCTCCGGGTATGGGTATTCAGTGGAGACACTGATTCTGTAGTTCCACTGACCGCAACTCGATATTCTATCGATGCCTTGAAGCTACCAACCATCATCAATTGGTATCCTTGGTATGATAGTGGCAAGGTTGGTGGATGGAGTCAAGTTTATAAAGGGCTCACATTGGTAACAATAAGAGGTGCTGGACACGAGGTTCCTCTTCATAAGCCCCGCGAAGCGTTTATTCTTTTTAGATCATTCTTGGAGAACAAGGACATGCCATCATCAAGCTAA
Protein sequence

>Msa0662370

MGYSIFYIVLFVSIFCVGISLASSIEDQKRDKIKQLPGQPKNVGFEQYSGYVTVNEQNGRALFYWLIEAPLNRGPNLRPLVLWLNGGPGCSSIAYGASEEIGPFRIRPDGKSLFLNRYAWNNLANILFLDSPAGVGFSYCTKTTDLYTFGDQKTAEDAYIFLVKWFERFPQYKHREFYIAGESYAGHYVPQLAQLVYQRNKGIDKPVINFKGFMVGNGVTDDYHDYVGTFEYWWTHGLISDSTYRILRIACDFGSSQHPSVQCFQALRVAVAEQGNIDPYSIYTPPCNNTASLRSGLNGRYPWMSRAYDPCTERHSDVYFNRPEVQKALHANVTGIPYIWKTCSDIVGNYWTDSPLSMLPIYHELINAGLRVWVFSGDTDSVVPLTATRYSIDALKLPTIINWYPWYDSGKVGGWSQVYKGLTLVTIRGAGHEVPLHKPREAFILFRSFLENKDMPSSS*