Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1458280 | XP_003629898.1 | 83.088 | 136 | 17 | 4 | 6 | 138 | 3 | 135 | 5.05e-60 | 195 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1458280 | sp|Q94AL8|CRIM1_ARATH | 35.075 | 134 | 66 | 4 | 13 | 138 | 5 | 125 | 5.53e-13 | 66.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1458280 | G7L817 | 83.088 | 136 | 17 | 4 | 6 | 138 | 3 | 135 | 2.41e-60 | 195 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000310 | Msa1458280 | 0.832345 | 9.968576e-56 | -8.615850e-47 |
| Msa0657880 | Msa1458280 | 0.897908 | 8.900904e-77 | -8.615850e-47 |
| Msa0701780 | Msa1458280 | 0.953117 | 5.550284e-111 | -8.615850e-47 |
| Msa1296600 | Msa1458280 | 0.833590 | 4.885640e-56 | -8.615850e-47 |
| Msa1329010 | Msa1458280 | 0.831184 | 1.927868e-55 | -8.615850e-47 |
| Msa1330670 | Msa1458280 | 0.808459 | 3.065441e-50 | -8.615850e-47 |
| Msa1204380 | Msa1458280 | 0.822069 | 2.885115e-53 | -8.615850e-47 |
| Msa1215470 | Msa1458280 | 0.804445 | 2.152525e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1458280 | MtrunA17_Chr8g0380221 | 83.088 | 136 | 17 | 4 | 6 | 138 | 3 | 135 | 4.65e-64 | 195 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1458280 | AT1G29395.1 | 35.075 | 134 | 66 | 4 | 13 | 138 | 5 | 125 | 5.63e-14 | 66.6 |
| Msa1458280 | AT1G29390.1 | 42.857 | 84 | 47 | 1 | 55 | 138 | 44 | 126 | 1.55e-13 | 65.5 |
| Msa1458280 | AT1G29390.2 | 42.105 | 76 | 35 | 1 | 72 | 138 | 8 | 83 | 4.86e-13 | 63.2 |
Find 29 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGGAACAATAAATGATTT+TGG | 0.211324 | tig0039512:+194 | Msa1458280:intergenic |
| AAATCCGTTGGTGGTAGTTT+TGG | 0.300811 | tig0039512:+469 | Msa1458280:intergenic |
| GTAGAGATCCACTGAATATT+AGG | 0.308778 | tig0039512:+407 | Msa1458280:intergenic |
| CTGTTGTTCGGGTGCGGTTG+AGG | 0.353834 | tig0039512:+688 | Msa1458280:intergenic |
| AGATCCTTCACTCGTCATTA+CGG | 0.405245 | tig0039512:-512 | Msa1458280:CDS |
| GGGGCTTGCAAAGCAAATAA+TGG | 0.425620 | tig0039512:+176 | Msa1458280:intergenic |
| ATGATGATGGATCTGTTGTT+CGG | 0.439996 | tig0039512:+676 | Msa1458280:intergenic |
| CCTGAGGCATAGGGGATTGA+AGG | 0.457606 | tig0039512:+620 | Msa1458280:intergenic |
| ATTAGGAGTAGTGAAAGGAT+AGG | 0.461661 | tig0039512:+424 | Msa1458280:intergenic |
| GAAAGGATAGGGAGCGTAAG+AGG | 0.461907 | tig0039512:+436 | Msa1458280:intergenic |
| CCTTCAATCCCCTATGCCTC+AGG | 0.483755 | tig0039512:-620 | Msa1458280:intron |
| TACCAGGTACCTGAGGCATA+GGG | 0.493016 | tig0039512:+611 | Msa1458280:intergenic |
| TGATGATGGATCTGTTGTTC+GGG | 0.494670 | tig0039512:+677 | Msa1458280:intergenic |
| TTAGGAGTAGTGAAAGGATA+GGG | 0.507367 | tig0039512:+425 | Msa1458280:intergenic |
| ATCAATGACGTACTTTATCC+AGG | 0.509966 | tig0039512:+139 | Msa1458280:intergenic |
| AAGGAGAGCGATGATGATGA+TGG | 0.522436 | tig0039512:+663 | Msa1458280:intergenic |
| ATGGATCTGTTGTTCGGGTG+CGG | 0.525574 | tig0039512:+682 | Msa1458280:intergenic |
| TGCATTTACAGTTGAAGTTG+AGG | 0.526115 | tig0039512:+731 | Msa1458280:intergenic |
| GTACCAGGTACCTGAGGCAT+AGG | 0.529583 | tig0039512:+610 | Msa1458280:intergenic |
| TATTCCGTAATGACGAGTGA+AGG | 0.557475 | tig0039512:+508 | Msa1458280:intergenic |
| GCGAGTGCGAGGGTGAGAGA+AGG | 0.562288 | tig0039512:+644 | Msa1458280:intergenic |
| GGATTGAAGGTGCGAGTGCG+AGG | 0.574816 | tig0039512:+633 | Msa1458280:intergenic |
| TCACTACTCCTAATATTCAG+TGG | 0.580865 | tig0039512:-415 | Msa1458280:CDS |
| GATTGAAGGTGCGAGTGCGA+GGG | 0.588182 | tig0039512:+634 | Msa1458280:intergenic |
| GGAAAACACACGAAATCCGT+TGG | 0.618951 | tig0039512:+457 | Msa1458280:intergenic |
| AAGTCCAAAACTACCACCAA+CGG | 0.628404 | tig0039512:-473 | Msa1458280:CDS |
| AAACACACGAAATCCGTTGG+TGG | 0.638061 | tig0039512:+460 | Msa1458270:intergenic |
| TGAATATTAGGAGTAGTGAA+AGG | 0.638291 | tig0039512:+419 | Msa1458270:intergenic |
| ACCAGGTACCTGAGGCATAG+GGG | 0.660783 | tig0039512:+612 | Msa1458270:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATAATTAAATTGAGAGAG+AGG | + | tig0039512:678-697 | Msa1458280:intergenic | 20.0% |
| !! | CTCTCTCAATTTAATTATTA+TGG | - | tig0039512:678-697 | Msa1458280:CDS | 20.0% |
| !! | ATGAATATTCAAAATCTTGT+AGG | - | tig0039512:793-812 | Msa1458280:exon | 20.0% |
| !! | TGAATATTCAAAATCTTGTA+GGG | - | tig0039512:794-813 | Msa1458280:exon | 20.0% |
| !!! | AATGGAACAATAAATGATTT+TGG | + | tig0039512:849-868 | Msa1458280:intergenic | 20.0% |
| !! | TAATAATTAAATTGAGAGAG+AGG | + | tig0039512:678-697 | Msa1458280:intergenic | 20.0% |
| !! | CTCTCTCAATTTAATTATTA+TGG | - | tig0039512:678-697 | Msa1458280:CDS | 20.0% |
| !! | ATGAATATTCAAAATCTTGT+AGG | - | tig0039512:793-812 | Msa1458280:exon | 20.0% |
| !! | TGAATATTCAAAATCTTGTA+GGG | - | tig0039512:794-813 | Msa1458280:exon | 20.0% |
| !!! | AATGGAACAATAAATGATTT+TGG | + | tig0039512:849-868 | Msa1458280:intergenic | 20.0% |
| AAAAGAGTAGATGAGAATGT+AGG | + | tig0039512:455-474 | Msa1458280:intergenic | 30.0% | |
| ATCAGAGAGAGGAAATAAAA+AGG | + | tig0039512:482-501 | Msa1458280:intergenic | 30.0% | |
| TGAATATTAGGAGTAGTGAA+AGG | + | tig0039512:624-643 | Msa1458280:intergenic | 30.0% | |
| CATAAATGAAGAACGAAAGA+CGG | + | tig0039512:754-773 | Msa1458280:intergenic | 30.0% | |
| !! | ATCTTGTAGGGTTTTAATAC+TGG | - | tig0039512:806-825 | Msa1458280:five_prime_UTR | 30.0% |
| AAAAGAGTAGATGAGAATGT+AGG | + | tig0039512:455-474 | Msa1458280:intergenic | 30.0% | |
| ATCAGAGAGAGGAAATAAAA+AGG | + | tig0039512:482-501 | Msa1458280:intergenic | 30.0% | |
| TGAATATTAGGAGTAGTGAA+AGG | + | tig0039512:624-643 | Msa1458280:intergenic | 30.0% | |
| CATAAATGAAGAACGAAAGA+CGG | + | tig0039512:754-773 | Msa1458280:intergenic | 30.0% | |
| !! | ATCTTGTAGGGTTTTAATAC+TGG | - | tig0039512:806-825 | Msa1458280:five_prime_UTR | 30.0% |
| TCATCATCATCATCATCGTT+AGG | - | tig0039512:269-288 | Msa1458280:intron | 35.0% | |
| CATCATCATCATCATCGTTA+GGG | - | tig0039512:270-289 | Msa1458280:intron | 35.0% | |
| TGCATTTACAGTTGAAGTTG+AGG | + | tig0039512:312-331 | Msa1458280:intergenic | 35.0% | |
| !! | ATGATGATGGATCTGTTGTT+CGG | + | tig0039512:367-386 | Msa1458280:intergenic | 35.0% |
| TTAGGAGTAGTGAAAGGATA+GGG | + | tig0039512:618-637 | Msa1458280:intergenic | 35.0% | |
| ATTAGGAGTAGTGAAAGGAT+AGG | + | tig0039512:619-638 | Msa1458280:intergenic | 35.0% | |
| TCACTACTCCTAATATTCAG+TGG | - | tig0039512:625-644 | Msa1458280:CDS | 35.0% | |
| GTAGAGATCCACTGAATATT+AGG | + | tig0039512:636-655 | Msa1458280:intergenic | 35.0% | |
| TCATCAGCACATAACAGATT+TGG | + | tig0039512:777-796 | Msa1458280:intergenic | 35.0% | |
| !! | TAGGGTTTTAATACTGGCAA+AGG | - | tig0039512:812-831 | Msa1458280:five_prime_UTR | 35.0% |
| !! | AGGGTTTTAATACTGGCAAA+GGG | - | tig0039512:813-832 | Msa1458280:five_prime_UTR | 35.0% |
| TCATCATCATCATCATCGTT+AGG | - | tig0039512:269-288 | Msa1458280:intron | 35.0% | |
| CATCATCATCATCATCGTTA+GGG | - | tig0039512:270-289 | Msa1458280:intron | 35.0% | |
| TGCATTTACAGTTGAAGTTG+AGG | + | tig0039512:312-331 | Msa1458280:intergenic | 35.0% | |
| !! | ATGATGATGGATCTGTTGTT+CGG | + | tig0039512:367-386 | Msa1458280:intergenic | 35.0% |
| TTAGGAGTAGTGAAAGGATA+GGG | + | tig0039512:618-637 | Msa1458280:intergenic | 35.0% | |
| ATTAGGAGTAGTGAAAGGAT+AGG | + | tig0039512:619-638 | Msa1458280:intergenic | 35.0% | |
| TCACTACTCCTAATATTCAG+TGG | - | tig0039512:625-644 | Msa1458280:CDS | 35.0% | |
| GTAGAGATCCACTGAATATT+AGG | + | tig0039512:636-655 | Msa1458280:intergenic | 35.0% | |
| TCATCAGCACATAACAGATT+TGG | + | tig0039512:777-796 | Msa1458280:intergenic | 35.0% | |
| !! | TAGGGTTTTAATACTGGCAA+AGG | - | tig0039512:812-831 | Msa1458280:five_prime_UTR | 35.0% |
| !! | AGGGTTTTAATACTGGCAAA+GGG | - | tig0039512:813-832 | Msa1458280:five_prime_UTR | 35.0% |
| !! | TGATGATGGATCTGTTGTTC+GGG | + | tig0039512:366-385 | Msa1458280:intergenic | 40.0% |
| TAGATGAGAATGTAGGTACC+AGG | + | tig0039512:448-467 | Msa1458280:intergenic | 40.0% | |
| ! | CTGATCGTGTTTCTGTTGTT+TGG | - | tig0039512:497-516 | Msa1458280:CDS | 40.0% |
| AGATCCTTCACTCGTCATTA+CGG | - | tig0039512:528-547 | Msa1458280:intron | 40.0% | |
| TATTCCGTAATGACGAGTGA+AGG | + | tig0039512:535-554 | Msa1458280:intergenic | 40.0% | |
| AAGTCCAAAACTACCACCAA+CGG | - | tig0039512:567-586 | Msa1458280:intron | 40.0% | |
| !!! | AAATCCGTTGGTGGTAGTTT+TGG | + | tig0039512:574-593 | Msa1458280:intergenic | 40.0% |
| !! | TGATGATGGATCTGTTGTTC+GGG | + | tig0039512:366-385 | Msa1458280:intergenic | 40.0% |
| TAGATGAGAATGTAGGTACC+AGG | + | tig0039512:448-467 | Msa1458280:intergenic | 40.0% | |
| ! | CTGATCGTGTTTCTGTTGTT+TGG | - | tig0039512:497-516 | Msa1458280:CDS | 40.0% |
| AGATCCTTCACTCGTCATTA+CGG | - | tig0039512:528-547 | Msa1458280:intron | 40.0% | |
| TATTCCGTAATGACGAGTGA+AGG | + | tig0039512:535-554 | Msa1458280:intergenic | 40.0% | |
| AAGTCCAAAACTACCACCAA+CGG | - | tig0039512:567-586 | Msa1458280:intron | 40.0% | |
| !!! | AAATCCGTTGGTGGTAGTTT+TGG | + | tig0039512:574-593 | Msa1458280:intergenic | 40.0% |
| ! | AAGGAGAGCGATGATGATGA+TGG | + | tig0039512:380-399 | Msa1458280:intergenic | 45.0% |
| ACAGAAACACGATCAGAGAG+AGG | + | tig0039512:493-512 | Msa1458280:intergenic | 45.0% | |
| !!! | GGTGGTAGTTTTGGACTTGT+CGG | + | tig0039512:565-584 | Msa1458280:intergenic | 45.0% |
| AAACACACGAAATCCGTTGG+TGG | + | tig0039512:583-602 | Msa1458280:intergenic | 45.0% | |
| GGAAAACACACGAAATCCGT+TGG | + | tig0039512:586-605 | Msa1458280:intergenic | 45.0% | |
| GGGGCTTGCAAAGCAAATAA+TGG | + | tig0039512:867-886 | Msa1458280:intergenic | 45.0% | |
| TCCAGGAAAAAACAGCTGCA+GGG | + | tig0039512:887-906 | Msa1458280:intergenic | 45.0% | |
| ATCCAGGAAAAAACAGCTGC+AGG | + | tig0039512:888-907 | Msa1458280:intergenic | 45.0% | |
| ! | AAGGAGAGCGATGATGATGA+TGG | + | tig0039512:380-399 | Msa1458280:intergenic | 45.0% |
| ACAGAAACACGATCAGAGAG+AGG | + | tig0039512:493-512 | Msa1458280:intergenic | 45.0% | |
| !!! | GGTGGTAGTTTTGGACTTGT+CGG | + | tig0039512:565-584 | Msa1458280:intergenic | 45.0% |
| AAACACACGAAATCCGTTGG+TGG | + | tig0039512:583-602 | Msa1458280:intergenic | 45.0% | |
| GGAAAACACACGAAATCCGT+TGG | + | tig0039512:586-605 | Msa1458280:intergenic | 45.0% | |
| GGGGCTTGCAAAGCAAATAA+TGG | + | tig0039512:867-886 | Msa1458280:intergenic | 45.0% | |
| TCCAGGAAAAAACAGCTGCA+GGG | + | tig0039512:887-906 | Msa1458280:intergenic | 45.0% | |
| ATCCAGGAAAAAACAGCTGC+AGG | + | tig0039512:888-907 | Msa1458280:intergenic | 45.0% | |
| ATGGATCTGTTGTTCGGGTG+CGG | + | tig0039512:361-380 | Msa1458280:intergenic | 50.0% | |
| TACCAGGTACCTGAGGCATA+GGG | + | tig0039512:432-451 | Msa1458280:intergenic | 50.0% | |
| ATGTAGGTACCAGGTACCTG+AGG | + | tig0039512:439-458 | Msa1458280:intergenic | 50.0% | |
| ! | GAAAGGATAGGGAGCGTAAG+AGG | + | tig0039512:607-626 | Msa1458280:intergenic | 50.0% |
| CCAGGAAAAAACAGCTGCAG+GGG | + | tig0039512:886-905 | Msa1458280:intergenic | 50.0% | |
| ATGGATCTGTTGTTCGGGTG+CGG | + | tig0039512:361-380 | Msa1458280:intergenic | 50.0% | |
| TACCAGGTACCTGAGGCATA+GGG | + | tig0039512:432-451 | Msa1458280:intergenic | 50.0% | |
| ATGTAGGTACCAGGTACCTG+AGG | + | tig0039512:439-458 | Msa1458280:intergenic | 50.0% | |
| ! | GAAAGGATAGGGAGCGTAAG+AGG | + | tig0039512:607-626 | Msa1458280:intergenic | 50.0% |
| CCAGGAAAAAACAGCTGCAG+GGG | + | tig0039512:886-905 | Msa1458280:intergenic | 50.0% | |
| ! | GATTGAAGGTGCGAGTGCGA+GGG | + | tig0039512:409-428 | Msa1458280:intergenic | 55.0% |
| CCTGAGGCATAGGGGATTGA+AGG | + | tig0039512:423-442 | Msa1458280:intergenic | 55.0% | |
| CCTTCAATCCCCTATGCCTC+AGG | - | tig0039512:420-439 | Msa1458280:CDS | 55.0% | |
| ACCAGGTACCTGAGGCATAG+GGG | + | tig0039512:431-450 | Msa1458280:intergenic | 55.0% | |
| GTACCAGGTACCTGAGGCAT+AGG | + | tig0039512:433-452 | Msa1458280:intergenic | 55.0% | |
| !!! | CCCCTGCAGCTGTTTTTTCC+TGG | - | tig0039512:883-902 | Msa1458280:five_prime_UTR | 55.0% |
| ! | GATTGAAGGTGCGAGTGCGA+GGG | + | tig0039512:409-428 | Msa1458280:intergenic | 55.0% |
| CCTGAGGCATAGGGGATTGA+AGG | + | tig0039512:423-442 | Msa1458280:intergenic | 55.0% | |
| CCTTCAATCCCCTATGCCTC+AGG | - | tig0039512:420-439 | Msa1458280:CDS | 55.0% | |
| ACCAGGTACCTGAGGCATAG+GGG | + | tig0039512:431-450 | Msa1458280:intergenic | 55.0% | |
| GTACCAGGTACCTGAGGCAT+AGG | + | tig0039512:433-452 | Msa1458280:intergenic | 55.0% | |
| !!! | CCCCTGCAGCTGTTTTTTCC+TGG | - | tig0039512:883-902 | Msa1458280:five_prime_UTR | 55.0% |
| CTGTTGTTCGGGTGCGGTTG+AGG | + | tig0039512:355-374 | Msa1458280:intergenic | 60.0% | |
| ! | GGATTGAAGGTGCGAGTGCG+AGG | + | tig0039512:410-429 | Msa1458280:intergenic | 60.0% |
| TCCCCTATGCCTCAGGTACC+TGG | - | tig0039512:427-446 | Msa1458280:CDS | 60.0% | |
| CTGTTGTTCGGGTGCGGTTG+AGG | + | tig0039512:355-374 | Msa1458280:intergenic | 60.0% | |
| ! | GGATTGAAGGTGCGAGTGCG+AGG | + | tig0039512:410-429 | Msa1458280:intergenic | 60.0% |
| TCCCCTATGCCTCAGGTACC+TGG | - | tig0039512:427-446 | Msa1458280:CDS | 60.0% | |
| GCGAGTGCGAGGGTGAGAGA+AGG | + | tig0039512:399-418 | Msa1458280:intergenic | 65.0% | |
| GCGAGTGCGAGGGTGAGAGA+AGG | + | tig0039512:399-418 | Msa1458280:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0039512 | gene | 154 | 908 | 154 | ID=Msa1458280;Name=Msa1458280 |
| tig0039512 | mRNA | 154 | 908 | 154 | ID=Msa1458280-mRNA-1;Parent=Msa1458280;Name=Msa1458280-mRNA-1;_AED=0.12;_eAED=0.12;_QI=106|1|0.66|1|1|1|3|0|138 |
| tig0039512 | exon | 154 | 247 | 154 | ID=Msa1458280-mRNA-1:exon:21680;Parent=Msa1458280-mRNA-1 |
| tig0039512 | exon | 395 | 532 | 395 | ID=Msa1458280-mRNA-1:exon:21679;Parent=Msa1458280-mRNA-1 |
| tig0039512 | exon | 621 | 908 | 621 | ID=Msa1458280-mRNA-1:exon:21678;Parent=Msa1458280-mRNA-1 |
| tig0039512 | five_prime_UTR | 803 | 908 | 803 | ID=Msa1458280-mRNA-1:five_prime_utr;Parent=Msa1458280-mRNA-1 |
| tig0039512 | CDS | 621 | 802 | 621 | ID=Msa1458280-mRNA-1:cds;Parent=Msa1458280-mRNA-1 |
| tig0039512 | CDS | 395 | 532 | 395 | ID=Msa1458280-mRNA-1:cds;Parent=Msa1458280-mRNA-1 |
| tig0039512 | CDS | 154 | 247 | 154 | ID=Msa1458280-mRNA-1:cds;Parent=Msa1458280-mRNA-1 |
| Gene Sequence |
| Protein sequence |
