AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1464100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1464100 MtrunA17_Chr1g0158661 69.672 122 36 1 1 122 152 272 8.04e-52 173
Msa1464100 MtrunA17_Chr1g0158631 76.147 109 25 1 1 109 159 266 1.27e-51 172
Msa1464100 MtrunA17_Chr1g0158571 64.286 98 31 2 13 110 1 94 8.60e-35 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 62 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCACAACTCTTGCATATC+TGG 0.189605 tig0043903:-3327 Msa1464100:intergenic
ACTAGCATTGTGAGTGAAAA+TGG 0.223619 tig0043903:+3277 Msa1464100:five_prime_UTR
CAATTGGTGGGTTGAATGTT+TGG 0.225192 tig0043903:-3574 Msa1464100:intergenic
TTGTTACTCTCACATCCTTC+TGG 0.271931 tig0043903:+3875 Msa1464100:CDS
GCTATGGATTTGAAATTTCC+TGG 0.305840 tig0043903:+4526 Msa1464100:CDS
TTCACTCACAATGCTAGTTT+TGG 0.307657 tig0043903:-3272 Msa1464100:intergenic
AAACCCACAACTTGGAATAT+TGG 0.322800 tig0043903:+3831 Msa1464100:CDS
CCGATTACATTGATTTATCT+AGG 0.323521 tig0043903:+3701 Msa1464100:five_prime_UTR
GTGAGGGAGAGAGGAGCAAT+TGG 0.327106 tig0043903:-3590 Msa1464100:intergenic
ATGGTTTCAACCTCTAACTT+TGG 0.331944 tig0043903:+3296 Msa1464100:five_prime_UTR
GTACACTCCATGTAAATTAC+AGG 0.363818 tig0043903:-3724 Msa1464100:intergenic
TGTTACTCTCACATCCTTCT+GGG 0.367632 tig0043903:+3876 Msa1464100:CDS
AGGGGAGGATATAACATTAT+AGG 0.375008 tig0043903:+3916 Msa1464100:CDS
CACAACATATATGTAATTGT+TGG 0.401837 tig0043903:+3808 Msa1464100:CDS
TAAGAAGAAGTGAGGGAGAG+AGG 0.410699 tig0043903:-3599 Msa1464100:intergenic
CCTAGATAAATCAATGTAAT+CGG 0.416399 tig0043903:-3701 Msa1464100:intergenic
AATTGGTGGGTTGAATGTTT+GGG 0.419800 tig0043903:-3573 Msa1464100:intergenic
GGGGAGCATATACTTACATC+AGG 0.453660 tig0043903:+3896 Msa1464100:CDS
ATCTAGGCCTGTAATTTACA+TGG 0.460518 tig0043903:+3717 Msa1464100:exon
GGCTCCAATATTCCAAGTTG+TGG 0.468868 tig0043903:-3835 Msa1464100:intergenic
TACATATCCACAACGTTACT+AGG 0.473319 tig0043903:-3754 Msa1464100:intergenic
AGGGAGAGAGGAGCAATTGG+TGG 0.474420 tig0043903:-3587 Msa1464100:intergenic
ATTGTTGGAAACCCACAACT+TGG 0.474548 tig0043903:+3823 Msa1464100:CDS
ACAGTATTGAATTTGTATGA+TGG 0.477569 tig0043903:+3496 Msa1464100:five_prime_UTR
GCATTGATCACAATGACAAA+AGG 0.483273 tig0043903:-4556 Msa1464100:intergenic
CAGGAAGAAGAACAAGTTTG+TGG 0.492234 tig0043903:-3389 Msa1464100:intergenic
ATTCAGAAGGCACTAGGCTA+TGG 0.492318 tig0043903:+4510 Msa1464100:CDS
GCTCCAATATTCCAAGTTGT+GGG 0.494268 tig0043903:-3834 Msa1464100:intergenic
ATCACAACTCTTGCATATCT+GGG 0.496177 tig0043903:-3326 Msa1464100:intergenic
AATGTAATCTTAAGTCAAGA+TGG 0.510542 tig0043903:-3224 Msa1464100:intergenic
AACAAAGGACTAAGGTCTTA+CGG 0.516938 tig0043903:-4623 Msa1464100:intergenic
ATGACAAAAGGGTTGCATCC+AGG 0.518026 tig0043903:-4544 Msa1464100:intergenic
TGCGGATACAACTTCATGCA+TGG 0.520666 tig0043903:+3780 Msa1464100:CDS
CAAACTTGTTCTTCTTCCTG+TGG 0.522063 tig0043903:+3392 Msa1464100:five_prime_UTR
TGCTACCTCAAGGACCCTAC+TGG 0.532171 tig0043903:+4576 Msa1464100:CDS
GAAAATGTAACAAAGGACTA+AGG 0.532231 tig0043903:-4631 Msa1464100:intergenic
CATTGATCACAATGACAAAA+GGG 0.540429 tig0043903:-4555 Msa1464100:intergenic
TTGCTGTATAAGAAGAAGTG+AGG 0.550159 tig0043903:-3607 Msa1464100:intergenic
TCTGAATGGATTAGTTACGT+TGG 0.558617 tig0043903:-3423 Msa1464100:intergenic
GGGAGAGAGGAGCAATTGGT+GGG 0.574563 tig0043903:-3586 Msa1464100:intergenic
AACACTGAATCTCGCCAGTA+GGG 0.577783 tig0043903:-4590 Msa1464100:intergenic
TGCTGTATAAGAAGAAGTGA+GGG 0.581666 tig0043903:-3606 Msa1464100:intergenic
TCCATTCAGATTAAAACATG+TGG 0.585132 tig0043903:+3436 Msa1464100:five_prime_UTR
TAACACTGAATCTCGCCAGT+AGG 0.586767 tig0043903:-4591 Msa1464100:intergenic
GCGGATACAACTTCATGCAT+GGG 0.595465 tig0043903:+3781 Msa1464100:CDS
GTTACTCTCACATCCTTCTG+GGG 0.604732 tig0043903:+3877 Msa1464100:CDS
TCTCGCCAGTAGGGTCCTTG+AGG 0.613174 tig0043903:-4581 Msa1464100:intergenic
ATCATACATCGATATTCAGA+AGG 0.615231 tig0043903:+4497 Msa1464100:CDS
TGGTAACAGTACGTACGAGT+TGG 0.621121 tig0043903:+3456 Msa1464100:five_prime_UTR
TTGTGATCAATGCTACCTCA+AGG 0.625096 tig0043903:+4566 Msa1464100:CDS
ATCGATATTCAGAAGGCACT+AGG 0.625875 tig0043903:+4504 Msa1464100:CDS
ACGTAATGAAAATGTAACAA+AGG 0.628114 tig0043903:-4638 Msa1464100:intergenic
AAGTCCACCTAGTAACGTTG+TGG 0.633982 tig0043903:+3747 Msa1464100:CDS
CGTTGTGGATATGTATTATG+CGG 0.639742 tig0043903:+3762 Msa1464100:CDS
GTAAGTATATGCTCCCCAGA+AGG 0.641943 tig0043903:-3890 Msa1464100:intergenic
GGGAGCATATACTTACATCA+GGG 0.649036 tig0043903:+3897 Msa1464100:CDS
GCATATACTTACATCAGGGG+AGG 0.651355 tig0043903:+3901 Msa1464100:CDS
ATATCCACAACGTTACTAGG+TGG 0.679995 tig0043903:-3751 Msa1464100:intergenic
ACAAGCTTAACACGGCATTG+AGG 0.681882 tig0043903:-3856 Msa1464100:intergenic
GTAATCGGAAAACTCAGACG+TGG 0.693678 tig0043903:-3686 Msa1464100:intergenic
TGAGAGTAACAAGCTTAACA+CGG 0.706562 tig0043903:-3864 Msa1464100:intergenic
GGAGCATATACTTACATCAG+GGG 0.716060 tig0043903:+3898 Msa1464100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTCTTTTTTTTTTTTCA+TGG - tig0043903:4168-4187 Msa1464100:intergenic 10.0%
!!! AATTTCTTTTTTTTTTTTCA+TGG - tig0043903:4168-4187 Msa1464100:intergenic 10.0%
!! TTTATTTACACTTAAATAGA+AGG + tig0043903:4336-4355 Msa1464100:intron 15.0%
!! TTTATTTACACTTAAATAGA+AGG + tig0043903:4336-4355 Msa1464100:intron 15.0%
!!! GTATGAATTAGTATCTTTTT+GGG - tig0043903:3641-3660 Msa1464100:intergenic 20.0%
!! TATAGACAAAAAAATCTACA+CGG - tig0043903:4451-4470 Msa1464100:intergenic 20.0%
!!! GTATGAATTAGTATCTTTTT+GGG - tig0043903:3641-3660 Msa1464100:intergenic 20.0%
!! TATAGACAAAAAAATCTACA+CGG - tig0043903:4451-4470 Msa1464100:intergenic 20.0%
! AATGTAATCTTAAGTCAAGA+TGG - tig0043903:3227-3246 Msa0189570:intergenic 25.0%
!! ACAGTATTGAATTTGTATGA+TGG + tig0043903:3496-3515 Msa1464100:five_prime_UTR 25.0%
!! CGTATGAATTAGTATCTTTT+TGG - tig0043903:3642-3661 Msa1464100:intergenic 25.0%
! CCTAGATAAATCAATGTAAT+CGG - tig0043903:3704-3723 Msa1464100:intergenic 25.0%
! CACAACATATATGTAATTGT+TGG + tig0043903:3808-3827 Msa1464100:CDS 25.0%
! ATATAGTCAAATTGAGTCAT+TGG - tig0043903:3972-3991 Msa1464100:intergenic 25.0%
! TTTGACTATATCACTATTGA+TGG + tig0043903:3981-4000 Msa1464100:intron 25.0%
! GACGATATACAATATATTGT+CGG + tig0043903:4038-4057 Msa1464100:intron 25.0%
! ACACTTAAATAGAAGGTAAA+CGG + tig0043903:4343-4362 Msa1464100:intron 25.0%
!!! ATCGATTTTGTCATCTAAAA+AGG - tig0043903:4390-4409 Msa1464100:intergenic 25.0%
! ATGTAATCTTAAGTCAAGAT+GGG - tig0043903:3226-3245 Msa1464100:intergenic 25.0%
! AATGTAATCTTAAGTCAAGA+TGG - tig0043903:3227-3246 Msa1464100:intergenic 25.0%
!! ACAGTATTGAATTTGTATGA+TGG + tig0043903:3496-3515 Msa1464100:five_prime_UTR 25.0%
!! CGTATGAATTAGTATCTTTT+TGG - tig0043903:3642-3661 Msa1464100:intergenic 25.0%
! CCTAGATAAATCAATGTAAT+CGG - tig0043903:3704-3723 Msa1464100:intergenic 25.0%
! CACAACATATATGTAATTGT+TGG + tig0043903:3808-3827 Msa1464100:CDS 25.0%
! ATATAGTCAAATTGAGTCAT+TGG - tig0043903:3972-3991 Msa1464100:intergenic 25.0%
! TTTGACTATATCACTATTGA+TGG + tig0043903:3981-4000 Msa1464100:intron 25.0%
! GACGATATACAATATATTGT+CGG + tig0043903:4038-4057 Msa1464100:intron 25.0%
! ACACTTAAATAGAAGGTAAA+CGG + tig0043903:4343-4362 Msa1464100:intron 25.0%
!!! ATCGATTTTGTCATCTAAAA+AGG - tig0043903:4390-4409 Msa1464100:intergenic 25.0%
TCCATTCAGATTAAAACATG+TGG + tig0043903:3436-3455 Msa1464100:five_prime_UTR 30.0%
!!! ACCACATGTTTTAATCTGAA+TGG - tig0043903:3440-3459 Msa1464100:intergenic 30.0%
!! CCGATTACATTGATTTATCT+AGG + tig0043903:3701-3720 Msa1464100:five_prime_UTR 30.0%
CACTTAAATAGAAGGTAAAC+GGG + tig0043903:4344-4363 Msa1464100:intron 30.0%
ATCATACATCGATATTCAGA+AGG + tig0043903:4497-4516 Msa1464100:CDS 30.0%
CATTGATCACAATGACAAAA+GGG - tig0043903:4558-4577 Msa1464100:intergenic 30.0%
GAAAATGTAACAAAGGACTA+AGG - tig0043903:4634-4653 Msa1464100:intergenic 30.0%
TCCATTCAGATTAAAACATG+TGG + tig0043903:3436-3455 Msa1464100:five_prime_UTR 30.0%
!!! ACCACATGTTTTAATCTGAA+TGG - tig0043903:3440-3459 Msa1464100:intergenic 30.0%
!! CCGATTACATTGATTTATCT+AGG + tig0043903:3701-3720 Msa1464100:five_prime_UTR 30.0%
CACTTAAATAGAAGGTAAAC+GGG + tig0043903:4344-4363 Msa1464100:intron 30.0%
ATCATACATCGATATTCAGA+AGG + tig0043903:4497-4516 Msa1464100:CDS 30.0%
CATTGATCACAATGACAAAA+GGG - tig0043903:4558-4577 Msa1464100:intergenic 30.0%
GAAAATGTAACAAAGGACTA+AGG - tig0043903:4634-4653 Msa1464100:intergenic 30.0%
!! GTGGTAGAGTACAGTTTTAA+AGG - tig0043903:3253-3272 Msa0189570:intergenic 35.0%
!! TTCACTCACAATGCTAGTTT+TGG - tig0043903:3275-3294 Msa0189570:intergenic 35.0%
ACTAGCATTGTGAGTGAAAA+TGG + tig0043903:3277-3296 Msa1464100:five_prime_UTR 35.0%
ATGGTTTCAACCTCTAACTT+TGG + tig0043903:3296-3315 Msa1464100:five_prime_UTR 35.0%
ATCACAACTCTTGCATATCT+GGG - tig0043903:3329-3348 Msa1464100:intergenic 35.0%
TATCACAACTCTTGCATATC+TGG - tig0043903:3330-3349 Msa1464100:intergenic 35.0%
!! GTTTGTGGATAAGTTTTGTG+TGG - tig0043903:3377-3396 Msa1464100:intergenic 35.0%
! TACGTTGGAAATTTTTCCAC+AGG - tig0043903:3411-3430 Msa1464100:intergenic 35.0%
!! TCTGAATGGATTAGTTACGT+TGG - tig0043903:3426-3445 Msa1464100:intergenic 35.0%
!! AATTGGTGGGTTGAATGTTT+GGG - tig0043903:3576-3595 Msa1464100:intergenic 35.0%
TGCTGTATAAGAAGAAGTGA+GGG - tig0043903:3609-3628 Msa1464100:intergenic 35.0%
TTGCTGTATAAGAAGAAGTG+AGG - tig0043903:3610-3629 Msa1464100:intergenic 35.0%
ATCTAGGCCTGTAATTTACA+TGG + tig0043903:3717-3736 Msa1464100:exon 35.0%
GTACACTCCATGTAAATTAC+AGG - tig0043903:3727-3746 Msa1464100:intergenic 35.0%
TACATATCCACAACGTTACT+AGG - tig0043903:3757-3776 Msa1464100:intergenic 35.0%
CGTTGTGGATATGTATTATG+CGG + tig0043903:3762-3781 Msa1464100:CDS 35.0%
AAACCCACAACTTGGAATAT+TGG + tig0043903:3831-3850 Msa1464100:CDS 35.0%
TGAGAGTAACAAGCTTAACA+CGG - tig0043903:3867-3886 Msa1464100:intergenic 35.0%
AGGGGAGGATATAACATTAT+AGG + tig0043903:3916-3935 Msa1464100:CDS 35.0%
! GAGTCATTGGTTATAGAAGA+AGG - tig0043903:3959-3978 Msa1464100:intergenic 35.0%
ATTGAATATCTTACGACCGA+TGG + tig0043903:4185-4204 Msa1464100:intron 35.0%
TCATAGTTGTCCTATTCCAT+CGG - tig0043903:4204-4223 Msa1464100:intergenic 35.0%
GGAATAGGACAACTATGATA+TGG + tig0043903:4206-4225 Msa1464100:intron 35.0%
TGTCATCTAAAAAGGTCTAG+CGG - tig0043903:4382-4401 Msa1464100:intergenic 35.0%
! GCTATGGATTTGAAATTTCC+TGG + tig0043903:4526-4545 Msa1464100:CDS 35.0%
! GCATTGATCACAATGACAAA+AGG - tig0043903:4559-4578 Msa1464100:intergenic 35.0%
AACAAAGGACTAAGGTCTTA+CGG - tig0043903:4626-4645 Msa1464100:intergenic 35.0%
!! GTGGTAGAGTACAGTTTTAA+AGG - tig0043903:3253-3272 Msa1464100:intergenic 35.0%
!! TTCACTCACAATGCTAGTTT+TGG - tig0043903:3275-3294 Msa1464100:intergenic 35.0%
ACTAGCATTGTGAGTGAAAA+TGG + tig0043903:3277-3296 Msa1464100:five_prime_UTR 35.0%
ATGGTTTCAACCTCTAACTT+TGG + tig0043903:3296-3315 Msa1464100:five_prime_UTR 35.0%
ATCACAACTCTTGCATATCT+GGG - tig0043903:3329-3348 Msa1464100:intergenic 35.0%
TATCACAACTCTTGCATATC+TGG - tig0043903:3330-3349 Msa1464100:intergenic 35.0%
!! GTTTGTGGATAAGTTTTGTG+TGG - tig0043903:3377-3396 Msa1464100:intergenic 35.0%
! TACGTTGGAAATTTTTCCAC+AGG - tig0043903:3411-3430 Msa1464100:intergenic 35.0%
!! TCTGAATGGATTAGTTACGT+TGG - tig0043903:3426-3445 Msa1464100:intergenic 35.0%
!! AATTGGTGGGTTGAATGTTT+GGG - tig0043903:3576-3595 Msa1464100:intergenic 35.0%
TGCTGTATAAGAAGAAGTGA+GGG - tig0043903:3609-3628 Msa1464100:intergenic 35.0%
TTGCTGTATAAGAAGAAGTG+AGG - tig0043903:3610-3629 Msa1464100:intergenic 35.0%
ATCTAGGCCTGTAATTTACA+TGG + tig0043903:3717-3736 Msa1464100:exon 35.0%
GTACACTCCATGTAAATTAC+AGG - tig0043903:3727-3746 Msa1464100:intergenic 35.0%
TACATATCCACAACGTTACT+AGG - tig0043903:3757-3776 Msa1464100:intergenic 35.0%
CGTTGTGGATATGTATTATG+CGG + tig0043903:3762-3781 Msa1464100:CDS 35.0%
AAACCCACAACTTGGAATAT+TGG + tig0043903:3831-3850 Msa1464100:CDS 35.0%
TGAGAGTAACAAGCTTAACA+CGG - tig0043903:3867-3886 Msa1464100:intergenic 35.0%
AGGGGAGGATATAACATTAT+AGG + tig0043903:3916-3935 Msa1464100:CDS 35.0%
! GAGTCATTGGTTATAGAAGA+AGG - tig0043903:3959-3978 Msa1464100:intergenic 35.0%
ATTGAATATCTTACGACCGA+TGG + tig0043903:4185-4204 Msa1464100:intron 35.0%
TCATAGTTGTCCTATTCCAT+CGG - tig0043903:4204-4223 Msa1464100:intergenic 35.0%
GGAATAGGACAACTATGATA+TGG + tig0043903:4206-4225 Msa1464100:intron 35.0%
TGTCATCTAAAAAGGTCTAG+CGG - tig0043903:4382-4401 Msa1464100:intergenic 35.0%
! GCTATGGATTTGAAATTTCC+TGG + tig0043903:4526-4545 Msa1464100:CDS 35.0%
! GCATTGATCACAATGACAAA+AGG - tig0043903:4559-4578 Msa1464100:intergenic 35.0%
AACAAAGGACTAAGGTCTTA+CGG - tig0043903:4626-4645 Msa1464100:intergenic 35.0%
!!! ACTCACAATGCTAGTTTTGG+TGG - tig0043903:3272-3291 Msa0189570:intergenic 40.0%
! GGGTTTTTCTCCAAAGTTAG+AGG - tig0043903:3309-3328 Msa1464100:intergenic 40.0%
CAGGAAGAAGAACAAGTTTG+TGG - tig0043903:3392-3411 Msa1464100:intergenic 40.0%
! CAAACTTGTTCTTCTTCCTG+TGG + tig0043903:3392-3411 Msa1464100:five_prime_UTR 40.0%
!! CAATTGGTGGGTTGAATGTT+TGG - tig0043903:3577-3596 Msa1464100:intergenic 40.0%
ATATCCACAACGTTACTAGG+TGG - tig0043903:3754-3773 Msa1464100:intergenic 40.0%
ATTGTTGGAAACCCACAACT+TGG + tig0043903:3823-3842 Msa1464100:CDS 40.0%
GCTCCAATATTCCAAGTTGT+GGG - tig0043903:3837-3856 Msa1464100:intergenic 40.0%
TTGTTACTCTCACATCCTTC+TGG + tig0043903:3875-3894 Msa1464100:CDS 40.0%
TGTTACTCTCACATCCTTCT+GGG + tig0043903:3876-3895 Msa1464100:CDS 40.0%
! GGGAGCATATACTTACATCA+GGG + tig0043903:3897-3916 Msa1464100:CDS 40.0%
GGAGCATATACTTACATCAG+GGG + tig0043903:3898-3917 Msa1464100:CDS 40.0%
TATCTTACGACCGATGGAAT+AGG + tig0043903:4191-4210 Msa1464100:intron 40.0%
!! ATCGATATTCAGAAGGCACT+AGG + tig0043903:4504-4523 Msa1464100:CDS 40.0%
TTGTGATCAATGCTACCTCA+AGG + tig0043903:4566-4585 Msa1464100:CDS 40.0%
!!! ACTCACAATGCTAGTTTTGG+TGG - tig0043903:3272-3291 Msa1464100:intergenic 40.0%
! GGGTTTTTCTCCAAAGTTAG+AGG - tig0043903:3309-3328 Msa1464100:intergenic 40.0%
CAGGAAGAAGAACAAGTTTG+TGG - tig0043903:3392-3411 Msa1464100:intergenic 40.0%
! CAAACTTGTTCTTCTTCCTG+TGG + tig0043903:3392-3411 Msa1464100:five_prime_UTR 40.0%
!! CAATTGGTGGGTTGAATGTT+TGG - tig0043903:3577-3596 Msa1464100:intergenic 40.0%
ATATCCACAACGTTACTAGG+TGG - tig0043903:3754-3773 Msa1464100:intergenic 40.0%
ATTGTTGGAAACCCACAACT+TGG + tig0043903:3823-3842 Msa1464100:CDS 40.0%
GCTCCAATATTCCAAGTTGT+GGG - tig0043903:3837-3856 Msa1464100:intergenic 40.0%
TTGTTACTCTCACATCCTTC+TGG + tig0043903:3875-3894 Msa1464100:CDS 40.0%
TGTTACTCTCACATCCTTCT+GGG + tig0043903:3876-3895 Msa1464100:CDS 40.0%
! GGGAGCATATACTTACATCA+GGG + tig0043903:3897-3916 Msa1464100:CDS 40.0%
GGAGCATATACTTACATCAG+GGG + tig0043903:3898-3917 Msa1464100:CDS 40.0%
TATCTTACGACCGATGGAAT+AGG + tig0043903:4191-4210 Msa1464100:intron 40.0%
!! ATCGATATTCAGAAGGCACT+AGG + tig0043903:4504-4523 Msa1464100:CDS 40.0%
TTGTGATCAATGCTACCTCA+AGG + tig0043903:4566-4585 Msa1464100:CDS 40.0%
TGGTAACAGTACGTACGAGT+TGG + tig0043903:3456-3475 Msa1464100:five_prime_UTR 45.0%
TAAGAAGAAGTGAGGGAGAG+AGG - tig0043903:3602-3621 Msa1464100:intergenic 45.0%
GTAATCGGAAAACTCAGACG+TGG - tig0043903:3689-3708 Msa1464100:intergenic 45.0%
AAGTCCACCTAGTAACGTTG+TGG + tig0043903:3747-3766 Msa1464100:CDS 45.0%
TGCGGATACAACTTCATGCA+TGG + tig0043903:3780-3799 Msa1464100:CDS 45.0%
GCGGATACAACTTCATGCAT+GGG + tig0043903:3781-3800 Msa1464100:CDS 45.0%
GGCTCCAATATTCCAAGTTG+TGG - tig0043903:3838-3857 Msa1464100:intergenic 45.0%
ACAAGCTTAACACGGCATTG+AGG - tig0043903:3859-3878 Msa1464100:intergenic 45.0%
GTTACTCTCACATCCTTCTG+GGG + tig0043903:3877-3896 Msa1464100:CDS 45.0%
GTAAGTATATGCTCCCCAGA+AGG - tig0043903:3893-3912 Msa1464100:intergenic 45.0%
! GGGGAGCATATACTTACATC+AGG + tig0043903:3896-3915 Msa1464100:CDS 45.0%
GCATATACTTACATCAGGGG+AGG + tig0043903:3901-3920 Msa1464100:CDS 45.0%
!! ATTCAGAAGGCACTAGGCTA+TGG + tig0043903:4510-4529 Msa1464100:CDS 45.0%
ATGACAAAAGGGTTGCATCC+AGG - tig0043903:4547-4566 Msa1464100:intergenic 45.0%
AACACTGAATCTCGCCAGTA+GGG - tig0043903:4593-4612 Msa1464100:intergenic 45.0%
TAACACTGAATCTCGCCAGT+AGG - tig0043903:4594-4613 Msa1464100:intergenic 45.0%
TGGTAACAGTACGTACGAGT+TGG + tig0043903:3456-3475 Msa1464100:five_prime_UTR 45.0%
TAAGAAGAAGTGAGGGAGAG+AGG - tig0043903:3602-3621 Msa1464100:intergenic 45.0%
GTAATCGGAAAACTCAGACG+TGG - tig0043903:3689-3708 Msa1464100:intergenic 45.0%
AAGTCCACCTAGTAACGTTG+TGG + tig0043903:3747-3766 Msa1464100:CDS 45.0%
TGCGGATACAACTTCATGCA+TGG + tig0043903:3780-3799 Msa1464100:CDS 45.0%
GCGGATACAACTTCATGCAT+GGG + tig0043903:3781-3800 Msa1464100:CDS 45.0%
GGCTCCAATATTCCAAGTTG+TGG - tig0043903:3838-3857 Msa1464100:intergenic 45.0%
ACAAGCTTAACACGGCATTG+AGG - tig0043903:3859-3878 Msa1464100:intergenic 45.0%
GTTACTCTCACATCCTTCTG+GGG + tig0043903:3877-3896 Msa1464100:CDS 45.0%
GTAAGTATATGCTCCCCAGA+AGG - tig0043903:3893-3912 Msa1464100:intergenic 45.0%
! GGGGAGCATATACTTACATC+AGG + tig0043903:3896-3915 Msa1464100:CDS 45.0%
GCATATACTTACATCAGGGG+AGG + tig0043903:3901-3920 Msa1464100:CDS 45.0%
!! ATTCAGAAGGCACTAGGCTA+TGG + tig0043903:4510-4529 Msa1464100:CDS 45.0%
ATGACAAAAGGGTTGCATCC+AGG - tig0043903:4547-4566 Msa1464100:intergenic 45.0%
AACACTGAATCTCGCCAGTA+GGG - tig0043903:4593-4612 Msa1464100:intergenic 45.0%
TAACACTGAATCTCGCCAGT+AGG - tig0043903:4594-4613 Msa1464100:intergenic 45.0%
!!! ATAAATATTATTTCTTTTAT+AGG + tig0043903:4468-4487 Msa1464100:intron 5.0%
!!! ATAAATATTATTTCTTTTAT+AGG + tig0043903:4468-4487 Msa1464100:intron 5.0%
GGGAGAGAGGAGCAATTGGT+GGG - tig0043903:3589-3608 Msa1464100:intergenic 55.0%
AGGGAGAGAGGAGCAATTGG+TGG - tig0043903:3590-3609 Msa1464100:intergenic 55.0%
GTGAGGGAGAGAGGAGCAAT+TGG - tig0043903:3593-3612 Msa1464100:intergenic 55.0%
TGCTACCTCAAGGACCCTAC+TGG + tig0043903:4576-4595 Msa1464100:CDS 55.0%
GGGAGAGAGGAGCAATTGGT+GGG - tig0043903:3589-3608 Msa1464100:intergenic 55.0%
AGGGAGAGAGGAGCAATTGG+TGG - tig0043903:3590-3609 Msa1464100:intergenic 55.0%
GTGAGGGAGAGAGGAGCAAT+TGG - tig0043903:3593-3612 Msa1464100:intergenic 55.0%
TGCTACCTCAAGGACCCTAC+TGG + tig0043903:4576-4595 Msa1464100:CDS 55.0%
TCTCGCCAGTAGGGTCCTTG+AGG - tig0043903:4584-4603 Msa1464100:intergenic 60.0%
TCTCGCCAGTAGGGTCCTTG+AGG - tig0043903:4584-4603 Msa1464100:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0043903 gene 3224 4653 3224 ID=Msa1464100;Name=Msa1464100
tig0043903 mRNA 3224 4653 3224 ID=Msa1464100-mRNA-1;Parent=Msa1464100;Name=Msa1464100-mRNA-1;_AED=0.03;_eAED=0.03;_QI=512|1|0.5|1|1|1|2|0|122
tig0043903 exon 3224 3937 3224 ID=Msa1464100-mRNA-1:exon:24305;Parent=Msa1464100-mRNA-1
tig0043903 exon 4490 4653 4490 ID=Msa1464100-mRNA-1:exon:24306;Parent=Msa1464100-mRNA-1
tig0043903 five_prime_UTR 3224 3735 3224 ID=Msa1464100-mRNA-1:five_prime_utr;Parent=Msa1464100-mRNA-1
tig0043903 CDS 3736 3937 3736 ID=Msa1464100-mRNA-1:cds;Parent=Msa1464100-mRNA-1
tig0043903 CDS 4490 4653 4490 ID=Msa1464100-mRNA-1:cds;Parent=Msa1464100-mRNA-1
Gene Sequence

>Msa1464100

ATGGAGTGTACAAGTCCACCTAGTAACGTTGTGGATATGTATTATGCGGATACAACTTCATGCATGGGTCAACACAACATATATGTAATTGTTGGAAACCCACAACTTGGAATATTGGAGCCTCAATGCCGTGTTAAGCTTGTTACTCTCACATCCTTCTGGGGAGCATATACTTACATCAGGGGAGGATATAACATTATAGGCAACGTATCATACATCGATATTCAGAAGGCACTAGGCTATGGATTTGAAATTTCCTGGATGCAACCCTTTTGTCATTGTGATCAATGCTACCTCAAGGACCCTACTGGCGAGATTCAGTGTTACACAGAATACCGTAAGACCTTAGTCCTTTGTTACATTTTC
Protein sequence

>Msa1464100

MECTSPPSNVVDMYYADTTSCMGQHNIYVIVGNPQLGILEPQCRVKLVTLTSFWGAYTYIRGGYNIIGNVSYIDIQKALGYGFEISWMQPFCHCDQCYLKDPTGEIQCYTEYRKTLVLCYIF