AlfalfaGEDB | MS.gene000875

M. sativa cultivar XinJiangDaYe / MS.gene000875

KEGG: K02905
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01779.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000875.t1	XP_004508161.1	98.3	60	1	0	1	60	1	60	2.80E-23	117.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000875.t1	Q9M7X7	85.0	60	9	0	1	60	1	60	1.9e-20	99.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000875.t1	I3SZB6	98.3	60	1	0	1	60	1	60	2.1e-23	117.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene035826	MS.gene000875	PPI
MS.gene064351	MS.gene000875	PPI
MS.gene035828	MS.gene000875	PPI
MS.gene99872	MS.gene000875	PPI
MS.gene043643	MS.gene000875	PPI
MS.gene000875	MS.gene035825	PPI
MS.gene000875	MS.gene73093	PPI
MS.gene043645	MS.gene000875	PPI
MS.gene043593	MS.gene000875	PPI
MS.gene064353	MS.gene000875	PPI
MS.gene000875	MS.gene043595	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000875.t1	MTR_4g121877	98.333	60	1	0	1	60	1	60	7.50e-38	119
MS.gene000875.t1	MTR_4g121870	95.000	60	3	0	1	60	137	196	1.29e-36	120

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000875.t1	AT3G06680	85.000	60	9	1	60	23	82	2.96e-31	103
MS.gene000875.t1	AT3G06700	85.000	60	9	1	60	1	60	4.78e-31	102
MS.gene000875.t1	AT3G06700	85.000	60	9	1	60	1	60	4.78e-31	102
MS.gene000875.t1	AT3G06700	85.000	60	9	1	60	1	60	4.78e-31	102
MS.gene000875.t1	AT3G06680	85.000	60	9	1	60	1	60	6.50e-31	102
MS.gene000875.t1	AT3G06680	85.000	60	9	1	60	1	60	6.50e-31	102

Find 13 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGTGTGGCGGTGCCTCTT+TGG	0.285127	7.2:-67880478	None:intergenic
TATGCTTCCGTGCGTACCTC+TGG	0.442911	7.2:-67880632	None:intergenic
ACCGCCACACTTCCACCAAA+GGG	0.460737	7.2:+67880488	MS.gene000875:CDS
CGCTCACAACCAGTCTTACA+AGG	0.508995	7.2:+67880432	MS.gene000875:CDS
ATGATGAATACATACCCCTT+TGG	0.517971	7.2:-67880503	None:intergenic
CACCGCCACACTTCCACCAA+AGG	0.519273	7.2:+67880487	MS.gene000875:CDS
ATGAATACATACCCCTTTGG+TGG	0.547453	7.2:-67880500	None:intergenic
ATGGCATCAAGAAGCCAAAG+AGG	0.617235	7.2:+67880464	MS.gene000875:CDS
ATACCCCTTTGGTGGAAGTG+TGG	0.622392	7.2:-67880492	None:intergenic
CCCCTTTGGTGGAAGTGTGG+CGG	0.635065	7.2:-67880489	None:intergenic
CCGCCACACTTCCACCAAAG+GGG	0.674155	7.2:+67880489	MS.gene000875:CDS
CTTGTAAGACTGGTTGTGAG+CGG	0.674336	7.2:-67880431	None:intergenic
TGAGGAACCAGAGGTACGCA+CGG	0.675026	7.2:+67880625	MS.gene000875:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTGTGATTGATTACTGTAGA+TGG	+	chr7.2:67880590-67880609	MS.gene000875:intron	30.0%
	ATGATGAATACATACCCCTT+TGG	-	chr7.2:67880506-67880525	None:intergenic	35.0%
!	AGATGGATCCAAAGTTTTTG+AGG	+	chr7.2:67880607-67880626	MS.gene000875:intron	35.0%
!	CGGAAGCATAACAAGAAAAA+TGG	+	chr7.2:67880645-67880664	MS.gene000875:CDS	35.0%
!!!	CTTCTTGATGCCATTTTTGT+GGG	-	chr7.2:67880458-67880477	None:intergenic	35.0%
	ATGAATACATACCCCTTTGG+TGG	-	chr7.2:67880503-67880522	None:intergenic	40.0%
	TCTTACAAGGCCCACAAAAA+TGG	+	chr7.2:67880445-67880464	MS.gene000875:CDS	40.0%
!	CTCTGGTTCCTCAAAAACTT+TGG	-	chr7.2:67880618-67880637	None:intergenic	40.0%
!!	CAAAGTTTTTGAGGAACCAG+AGG	+	chr7.2:67880616-67880635	MS.gene000875:CDS	40.0%
!!!	GCTTCTTGATGCCATTTTTG+TGG	-	chr7.2:67880459-67880478	None:intergenic	40.0%
	ATGGCATCAAGAAGCCAAAG+AGG	+	chr7.2:67880464-67880483	MS.gene000875:CDS	45.0%
!	CTTGTAAGACTGGTTGTGAG+CGG	-	chr7.2:67880434-67880453	None:intergenic	45.0%
!!	TTTTGTGGGCCTTGTAAGAC+TGG	-	chr7.2:67880444-67880463	None:intergenic	45.0%
	ATACCCCTTTGGTGGAAGTG+TGG	-	chr7.2:67880495-67880514	None:intergenic	50.0%
	CGCTCACAACCAGTCTTACA+AGG	+	chr7.2:67880432-67880451	MS.gene000875:CDS	50.0%
	ACCGCCACACTTCCACCAAA+GGG	+	chr7.2:67880488-67880507	MS.gene000875:CDS	55.0%
	TATGCTTCCGTGCGTACCTC+TGG	-	chr7.2:67880635-67880654	None:intergenic	55.0%
!	TGAGGAACCAGAGGTACGCA+CGG	+	chr7.2:67880625-67880644	MS.gene000875:CDS	55.0%
	CACCGCCACACTTCCACCAA+AGG	+	chr7.2:67880487-67880506	MS.gene000875:CDS	60.0%
	CCGCCACACTTCCACCAAAG+GGG	+	chr7.2:67880489-67880508	MS.gene000875:CDS	60.0%
	GAAGTGTGGCGGTGCCTCTT+TGG	-	chr7.2:67880481-67880500	None:intergenic	60.0%
!	CCCCTTTGGTGGAAGTGTGG+CGG	-	chr7.2:67880492-67880511	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	67880409	67880689	67880409	ID=MS.gene000875
chr7.2	mRNA	67880409	67880689	67880409	ID=MS.gene000875.t1;Parent=MS.gene000875
chr7.2	exon	67880409	67880510	67880409	ID=MS.gene000875.t1.exon1;Parent=MS.gene000875.t1
chr7.2	CDS	67880409	67880510	67880409	ID=cds.MS.gene000875.t1;Parent=MS.gene000875.t1
chr7.2	exon	67880609	67880689	67880609	ID=MS.gene000875.t1.exon2;Parent=MS.gene000875.t1
chr7.2	CDS	67880609	67880689	67880609	ID=cds.MS.gene000875.t1;Parent=MS.gene000875.t1

Gene Sequence	>MS.gene000875 ATGGCGAAGTCGAAGAATCACACCGCTCACAACCAGTCTTACAAGGCCCACAAAAATGGCATCAAGAAGCCAAAGAGGCACCGCCACACTTCCACCAAAGGGGTATGTATTCATCATTCTTCTCTGATTTTTCAAACGATTGTTCTATTTTGTAATGAGATTTTAATTAATTGTTTATTTATTGTGATTGATTACTGTAGATGGATCCAAAGTTTTTGAGGAACCAGAGGTACGCACGGAAGCATAACAAGAAAAATGGTGAAATTGCAGAAGAAGAGTAA
Protein sequence	>MS.gene000875.t1 MAKSKNHTAHNQSYKAHKNGIKKPKRHRHTSTKGMDPKFLRNQRYARKHNKKNGEIAEEE