AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000876


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000876.t1 MTR_4g121870 96.721 61 2 0 1 61 137 197 4.71e-38 124
MS.gene000876.t1 MTR_4g121877 95.082 61 3 0 1 61 1 61 4.16e-37 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000876.t1 AT3G06700 86.885 61 8 0 1 61 1 61 3.03e-32 105
MS.gene000876.t1 AT3G06700 86.885 61 8 0 1 61 1 61 3.03e-32 105
MS.gene000876.t1 AT3G06700 86.885 61 8 0 1 61 1 61 3.03e-32 105
MS.gene000876.t1 AT3G06680 83.607 61 10 0 1 61 23 83 9.66e-32 105
MS.gene000876.t1 AT3G06680 83.607 61 10 0 1 61 1 61 2.47e-31 103
MS.gene000876.t1 AT3G06680 83.607 61 10 0 1 61 1 61 2.47e-31 103

Find 13 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGCATCAAGAAGCCAAAA+AGG 0.372719 7.2:+67875534 MS.gene000876:CDS
TCTTGTGAGCCTTGTAAGAC+TGG 0.428046 7.2:-67875511 None:intergenic
TGTGTTTCCTCGCATACCTC+TGG 0.457559 7.2:-67875698 None:intergenic
ATCACCACACTTCCACCAAA+GGG 0.482418 7.2:+67875558 MS.gene000876:CDS
CTGAGTAATACATACCCCTT+TGG 0.488635 7.2:-67875573 None:intergenic
CGCTCACAACCAGTCTTACA+AGG 0.489194 7.2:+67875502 MS.gene000876:CDS
TCTTACAAGGCTCACAAGAA+TGG 0.521704 7.2:+67875515 MS.gene000876:CDS
AGTAATACATACCCCTTTGG+TGG 0.557801 7.2:-67875570 None:intergenic
CATCACCACACTTCCACCAA+AGG 0.580490 7.2:+67875557 MS.gene000876:CDS
TAAGGAACCAGAGGTATGCG+AGG 0.651232 7.2:+67875691 MS.gene000876:CDS
CTTGTAAGACTGGTTGTGAG+CGG 0.674336 7.2:-67875501 None:intergenic
ATACCCCTTTGGTGGAAGTG+TGG 0.685351 7.2:-67875562 None:intergenic
TCACCACACTTCCACCAAAG+GGG 0.701603 7.2:+67875559 MS.gene000876:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTACTTTTATAATTGTAGA+TGG + chr7.2:67875656-67875675 MS.gene000876:intron 15.0%
AGGAAACACAACAAGAAAAA+TGG + chr7.2:67875711-67875730 MS.gene000876:CDS 30.0%
! AGATGGATCCAAAGTTTTTA+AGG + chr7.2:67875673-67875692 MS.gene000876:intron 30.0%
! CTCTGGTTCCTTAAAAACTT+TGG - chr7.2:67875684-67875703 None:intergenic 35.0%
!! CAAAGTTTTTAAGGAACCAG+AGG + chr7.2:67875682-67875701 MS.gene000876:CDS 35.0%
AGTAATACATACCCCTTTGG+TGG - chr7.2:67875573-67875592 None:intergenic 40.0%
ATGGCATCAAGAAGCCAAAA+AGG + chr7.2:67875534-67875553 MS.gene000876:CDS 40.0%
CTGAGTAATACATACCCCTT+TGG - chr7.2:67875576-67875595 None:intergenic 40.0%
TCTTACAAGGCTCACAAGAA+TGG + chr7.2:67875515-67875534 MS.gene000876:CDS 40.0%
ATCACCACACTTCCACCAAA+GGG + chr7.2:67875558-67875577 MS.gene000876:CDS 45.0%
! CTTGTAAGACTGGTTGTGAG+CGG - chr7.2:67875504-67875523 None:intergenic 45.0%
! TCTTGTGAGCCTTGTAAGAC+TGG - chr7.2:67875514-67875533 None:intergenic 45.0%
!! GAAGTGTGGTGATGCCTTTT+TGG - chr7.2:67875551-67875570 None:intergenic 45.0%
ATACCCCTTTGGTGGAAGTG+TGG - chr7.2:67875565-67875584 None:intergenic 50.0%
CATCACCACACTTCCACCAA+AGG + chr7.2:67875557-67875576 MS.gene000876:CDS 50.0%
CGCTCACAACCAGTCTTACA+AGG + chr7.2:67875502-67875521 MS.gene000876:CDS 50.0%
TCACCACACTTCCACCAAAG+GGG + chr7.2:67875559-67875578 MS.gene000876:CDS 50.0%
TGTGTTTCCTCGCATACCTC+TGG - chr7.2:67875701-67875720 None:intergenic 50.0%
! TAAGGAACCAGAGGTATGCG+AGG + chr7.2:67875691-67875710 MS.gene000876:CDS 50.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 67875479 67875758 67875479 ID=MS.gene000876
chr7.2 mRNA 67875479 67875758 67875479 ID=MS.gene000876.t1;Parent=MS.gene000876
chr7.2 exon 67875479 67875580 67875479 ID=MS.gene000876.t1.exon1;Parent=MS.gene000876.t1
chr7.2 CDS 67875479 67875580 67875479 ID=cds.MS.gene000876.t1;Parent=MS.gene000876.t1
chr7.2 exon 67875675 67875758 67875675 ID=MS.gene000876.t1.exon2;Parent=MS.gene000876.t1
chr7.2 CDS 67875675 67875758 67875675 ID=cds.MS.gene000876.t1;Parent=MS.gene000876.t1
Gene Sequence

>MS.gene000876

ATGGCGAAGTCGAAGAATCACACCGCTCACAACCAGTCTTACAAGGCTCACAAGAATGGCATCAAGAAGCCAAAAAGGCATCACCACACTTCCACCAAAGGGGTATGTATTACTCAGTTTTCAATTCTTTTATTGATTTTGAAATGATTGATTTCTATTGATGATTAATAATTTTATTTTACTTTTATAATTGTAGATGGATCCAAAGTTTTTAAGGAACCAGAGGTATGCGAGGAAACACAACAAGAAAAATGGTGAAATCGCTTCTGCTGAAGAGTAA

Protein sequence

>MS.gene000876.t1

MAKSKNHTAHNQSYKAHKNGIKKPKRHHHTSTKGMDPKFLRNQRYARKHNKKNGEIASAEE