Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000879.t1 | XP_004508161.1 | 98.3 | 60 | 1 | 0 | 1 | 60 | 1 | 60 | 2.80E-23 | 117.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000879.t1 | Q9M7X7 | 85.0 | 60 | 9 | 0 | 1 | 60 | 1 | 60 | 1.9e-20 | 99.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000879.t1 | I3SZB6 | 98.3 | 60 | 1 | 0 | 1 | 60 | 1 | 60 | 2.1e-23 | 117.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene064353 | MS.gene000879 | PPI |
| MS.gene000879 | MS.gene035825 | PPI |
| MS.gene035826 | MS.gene000879 | PPI |
| MS.gene064351 | MS.gene000879 | PPI |
| MS.gene035828 | MS.gene000879 | PPI |
| MS.gene000879 | MS.gene043595 | PPI |
| MS.gene99872 | MS.gene000879 | PPI |
| MS.gene043643 | MS.gene000879 | PPI |
| MS.gene043645 | MS.gene000879 | PPI |
| MS.gene000879 | MS.gene73093 | PPI |
| MS.gene043593 | MS.gene000879 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000879.t1 | MTR_4g121877 | 98.333 | 60 | 1 | 0 | 1 | 60 | 1 | 60 | 7.50e-38 | 119 |
| MS.gene000879.t1 | MTR_4g121870 | 95.000 | 60 | 3 | 0 | 1 | 60 | 137 | 196 | 1.29e-36 | 120 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000879.t1 | AT3G06680 | 85.000 | 60 | 9 | 0 | 1 | 60 | 23 | 82 | 2.96e-31 | 103 |
| MS.gene000879.t1 | AT3G06700 | 85.000 | 60 | 9 | 0 | 1 | 60 | 1 | 60 | 4.78e-31 | 102 |
| MS.gene000879.t1 | AT3G06700 | 85.000 | 60 | 9 | 0 | 1 | 60 | 1 | 60 | 4.78e-31 | 102 |
| MS.gene000879.t1 | AT3G06700 | 85.000 | 60 | 9 | 0 | 1 | 60 | 1 | 60 | 4.78e-31 | 102 |
| MS.gene000879.t1 | AT3G06680 | 85.000 | 60 | 9 | 0 | 1 | 60 | 1 | 60 | 6.50e-31 | 102 |
| MS.gene000879.t1 | AT3G06680 | 85.000 | 60 | 9 | 0 | 1 | 60 | 1 | 60 | 6.50e-31 | 102 |
Find 13 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTGTGACGATGCCTCTT+TGG | 0.332537 | 7.2:-67854686 | None:intergenic |
| CTCTGGTTCCTCAAGAACTT+TGG | 0.404366 | 7.2:-67854821 | None:intergenic |
| TCTTGTGAGCTTTGTAAGAC+TGG | 0.419661 | 7.2:-67854649 | None:intergenic |
| TATGCTTCCTTGCATACCTC+TGG | 0.420261 | 7.2:-67854838 | None:intergenic |
| ATCGTCACACTTCAACCAAA+GGG | 0.488627 | 7.2:+67854696 | MS.gene000879:CDS |
| ATGATGAATACATACCCCTT+TGG | 0.493133 | 7.2:-67854711 | None:intergenic |
| TCTTACAAAGCTCACAAGAA+TGG | 0.532217 | 7.2:+67854653 | MS.gene000879:CDS |
| CATCGTCACACTTCAACCAA+AGG | 0.595920 | 7.2:+67854695 | MS.gene000879:CDS |
| AGATGGATCCAAAGTTCTTG+AGG | 0.596761 | 7.2:+67854813 | MS.gene000879:intron |
| TGAGGAACCAGAGGTATGCA+AGG | 0.624057 | 7.2:+67854831 | MS.gene000879:CDS |
| CAAAGTTCTTGAGGAACCAG+AGG | 0.651910 | 7.2:+67854822 | MS.gene000879:CDS |
| TTTGTAAGACTGGTTGTGAG+CGG | 0.659865 | 7.2:-67854639 | None:intergenic |
| TCGTCACACTTCAACCAAAG+GGG | 0.708509 | 7.2:+67854697 | MS.gene000879:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTGTGATTGATTACTGTAGA+TGG | + | chr7.2:67854796-67854815 | MS.gene000879:intron | 30.0% | |
| ! | AGGAAGCATAACAAGAAAAA+TGG | + | chr7.2:67854851-67854870 | MS.gene000879:CDS | 30.0% |
| ATGATGAATACATACCCCTT+TGG | - | chr7.2:67854714-67854733 | None:intergenic | 35.0% | |
| TCTTACAAAGCTCACAAGAA+TGG | + | chr7.2:67854653-67854672 | MS.gene000879:CDS | 35.0% | |
| AGATGGATCCAAAGTTCTTG+AGG | + | chr7.2:67854813-67854832 | MS.gene000879:intron | 40.0% | |
| ATCGTCACACTTCAACCAAA+GGG | + | chr7.2:67854696-67854715 | MS.gene000879:CDS | 40.0% | |
| ATGGCATCAAAAAGCCAAAG+AGG | + | chr7.2:67854672-67854691 | MS.gene000879:CDS | 40.0% | |
| ! | TCTTGTGAGCTTTGTAAGAC+TGG | - | chr7.2:67854652-67854671 | None:intergenic | 40.0% |
| ! | TTTGTAAGACTGGTTGTGAG+CGG | - | chr7.2:67854642-67854661 | None:intergenic | 40.0% |
| CAAAGTTCTTGAGGAACCAG+AGG | + | chr7.2:67854822-67854841 | MS.gene000879:CDS | 45.0% | |
| CATCGTCACACTTCAACCAA+AGG | + | chr7.2:67854695-67854714 | MS.gene000879:CDS | 45.0% | |
| TATGCTTCCTTGCATACCTC+TGG | - | chr7.2:67854841-67854860 | None:intergenic | 45.0% | |
| TCGTCACACTTCAACCAAAG+GGG | + | chr7.2:67854697-67854716 | MS.gene000879:CDS | 45.0% | |
| ! | CTCTGGTTCCTCAAGAACTT+TGG | - | chr7.2:67854824-67854843 | None:intergenic | 45.0% |
| GAAGTGTGACGATGCCTCTT+TGG | - | chr7.2:67854689-67854708 | None:intergenic | 50.0% | |
| ! | TGAGGAACCAGAGGTATGCA+AGG | + | chr7.2:67854831-67854850 | MS.gene000879:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 67854617 | 67854895 | 67854617 | ID=MS.gene000879 |
| chr7.2 | mRNA | 67854617 | 67854895 | 67854617 | ID=MS.gene000879.t1;Parent=MS.gene000879 |
| chr7.2 | exon | 67854617 | 67854718 | 67854617 | ID=MS.gene000879.t1.exon1;Parent=MS.gene000879.t1 |
| chr7.2 | CDS | 67854617 | 67854718 | 67854617 | ID=cds.MS.gene000879.t1;Parent=MS.gene000879.t1 |
| chr7.2 | exon | 67854815 | 67854895 | 67854815 | ID=MS.gene000879.t1.exon2;Parent=MS.gene000879.t1 |
| chr7.2 | CDS | 67854815 | 67854895 | 67854815 | ID=cds.MS.gene000879.t1;Parent=MS.gene000879.t1 |
| Gene Sequence |
| Protein sequence |
