AlfalfaGEDB | MS.gene000881

M. sativa cultivar XinJiangDaYe / MS.gene000881

KEGG: K02905
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01779.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000881.t1	XP_004508161.1	96.7	61	2	0	1	61	1	61	2.20E-23	117.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000881.t1	Q9M7X7	82.0	61	11	0	1	61	1	61	5.7e-20	97.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000881.t1	I3SZB6	96.7	61	2	0	1	61	1	61	1.6e-23	117.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene043645	MS.gene000881	PPI
MS.gene043593	MS.gene000881	PPI
MS.gene064353	MS.gene000881	PPI
MS.gene035825	MS.gene000881	PPI
MS.gene73093	MS.gene000881	PPI
MS.gene043643	MS.gene000881	PPI
MS.gene035826	MS.gene000881	PPI
MS.gene064351	MS.gene000881	PPI
MS.gene035828	MS.gene000881	PPI
MS.gene99872	MS.gene000881	PPI
MS.gene043595	MS.gene000881	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000881.t1	MTR_4g121877	96.721	61	2	0	1	61	1	61	1.14e-37	119
MS.gene000881.t1	MTR_4g121870	91.803	61	5	0	1	61	137	197	1.10e-35	118

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000881.t1	AT3G06700	81.967	61	11	0	1	61	1	61	2.56e-30	100
MS.gene000881.t1	AT3G06700	81.967	61	11	0	1	61	1	61	2.56e-30	100
MS.gene000881.t1	AT3G06700	81.967	61	11	0	1	61	1	61	2.56e-30	100
MS.gene000881.t1	AT3G06680	81.967	61	11	0	1	61	23	83	3.31e-30	101
MS.gene000881.t1	AT3G06680	81.967	61	11	0	1	61	1	61	6.66e-30	100
MS.gene000881.t1	AT3G06680	81.967	61	11	0	1	61	1	61	6.66e-30	100

Find 13 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTTGTGAGCCTTGTAAAAC+TGG	0.320941	7.2:-67843903	None:intergenic
GAAGTGTGGCGATGCCTCTT+TGG	0.338170	7.2:-67843940	None:intergenic
CTGATAAATACAAACTCCTT+TGG	0.392523	7.2:-67843965	None:intergenic
CTCTGGTTCCTCAAGAACTT+TGG	0.404366	7.2:-67844071	None:intergenic
TGTGCTTCCTTGCATACCTC+TGG	0.438138	7.2:-67844088	None:intergenic
ATAAATACAAACTCCTTTGG+TGG	0.508253	7.2:-67843962	None:intergenic
AACAATTTACTCTTCTTCAG+TGG	0.567212	7.2:-67844132	None:intergenic
AGATGGATCCAAAGTTCTTG+AGG	0.579402	7.2:+67844063	MS.gene000881:intron
CATCGCCACACTTCCACCAA+AGG	0.596196	7.2:+67843949	MS.gene000881:CDS
AAACTCCTTTGGTGGAAGTG+TGG	0.614881	7.2:-67843954	None:intergenic
TGAGGAACCAGAGGTATGCA+AGG	0.624057	7.2:+67844081	MS.gene000881:CDS
ATGGCATCAAGAAGCCAAAG+AGG	0.631544	7.2:+67843926	MS.gene000881:CDS
CAAAGTTCTTGAGGAACCAG+AGG	0.651910	7.2:+67844072	MS.gene000881:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTATTTTTATAATTGTAGA+TGG	+	chr7.2:67844046-67844065	MS.gene000881:intron	10.0%
	ATAAATACAAACTCCTTTGG+TGG	-	chr7.2:67843965-67843984	None:intergenic	30.0%
	CTGATAAATACAAACTCCTT+TGG	-	chr7.2:67843968-67843987	None:intergenic	30.0%
!	AGGAAGCACAACAAGAAAAA+CGG	+	chr7.2:67844101-67844120	MS.gene000881:CDS	35.0%
!	TTTTACAAGGCTCACAAGAA+TGG	+	chr7.2:67843907-67843926	MS.gene000881:CDS	35.0%
	AGATGGATCCAAAGTTCTTG+AGG	+	chr7.2:67844063-67844082	MS.gene000881:intron	40.0%
!	TCTTGTGAGCCTTGTAAAAC+TGG	-	chr7.2:67843906-67843925	None:intergenic	40.0%
	AAACTCCTTTGGTGGAAGTG+TGG	-	chr7.2:67843957-67843976	None:intergenic	45.0%
	ATGGCATCAAGAAGCCAAAG+AGG	+	chr7.2:67843926-67843945	MS.gene000881:CDS	45.0%
	CAAAGTTCTTGAGGAACCAG+AGG	+	chr7.2:67844072-67844091	MS.gene000881:CDS	45.0%
!	CGCTCACAACCAGTTTTACA+AGG	+	chr7.2:67843894-67843913	MS.gene000881:CDS	45.0%
!	CTCTGGTTCCTCAAGAACTT+TGG	-	chr7.2:67844074-67844093	None:intergenic	45.0%
	TGTGCTTCCTTGCATACCTC+TGG	-	chr7.2:67844091-67844110	None:intergenic	50.0%
!	TGAGGAACCAGAGGTATGCA+AGG	+	chr7.2:67844081-67844100	MS.gene000881:CDS	50.0%
	CATCGCCACACTTCCACCAA+AGG	+	chr7.2:67843949-67843968	MS.gene000881:CDS	55.0%
	GAAGTGTGGCGATGCCTCTT+TGG	-	chr7.2:67843943-67843962	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	67843871	67844148	67843871	ID=MS.gene000881
chr7.2	mRNA	67843871	67844148	67843871	ID=MS.gene000881.t1;Parent=MS.gene000881
chr7.2	exon	67843871	67843972	67843871	ID=MS.gene000881.t1.exon1;Parent=MS.gene000881.t1
chr7.2	CDS	67843871	67843972	67843871	ID=cds.MS.gene000881.t1;Parent=MS.gene000881.t1
chr7.2	exon	67844065	67844148	67844065	ID=MS.gene000881.t1.exon2;Parent=MS.gene000881.t1
chr7.2	CDS	67844065	67844148	67844065	ID=cds.MS.gene000881.t1;Parent=MS.gene000881.t1

Gene Sequence	>MS.gene000881 ATGGCGAAGTCGAAGAATCACAACGCTCACAACCAGTTTTACAAGGCTCACAAGAATGGCATCAAGAAGCCAAAGAGGCATCGCCACACTTCCACCAAAGGAGTTTGTATTTATCAGTTTTCAATTCTTTTCTGAATTTGAAATGATTGATTTCTATTGATGATTAGTGATTTACTTTATTTTTATAATTGTAGATGGATCCAAAGTTCTTGAGGAACCAGAGGTATGCAAGGAAGCACAACAAGAAAAACGGTGAAATCGCCACTGAAGAAGAGTAA
Protein sequence	>MS.gene000881.t1 MAKSKNHNAHNQFYKAHKNGIKKPKRHRHTSTKGMDPKFLRNQRYARKHNKKNGEIATEEE

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