Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000883.t1 | XP_004508161.1 | 96.7 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 1.40E-22 | 114.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000883.t1 | Q9M7X7 | 83.3 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 9.6e-20 | 96.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000883.t1 | I3SZB6 | 96.7 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 1.0e-22 | 114.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene035825 | MS.gene000883 | PPI |
| MS.gene73093 | MS.gene000883 | PPI |
| MS.gene043643 | MS.gene000883 | PPI |
| MS.gene035826 | MS.gene000883 | PPI |
| MS.gene064351 | MS.gene000883 | PPI |
| MS.gene035828 | MS.gene000883 | PPI |
| MS.gene99872 | MS.gene000883 | PPI |
| MS.gene043595 | MS.gene000883 | PPI |
| MS.gene043645 | MS.gene000883 | PPI |
| MS.gene043593 | MS.gene000883 | PPI |
| MS.gene064353 | MS.gene000883 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000883.t1 | MTR_4g121877 | 96.667 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 5.36e-37 | 117 |
| MS.gene000883.t1 | MTR_4g121870 | 93.333 | 60 | 4 | 0 | 1 | 60 | 137 | 196 | 8.56e-36 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000883.t1 | AT3G06680 | 83.333 | 60 | 10 | 0 | 1 | 60 | 23 | 82 | 1.68e-30 | 102 |
| MS.gene000883.t1 | AT3G06700 | 83.333 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 2.51e-30 | 100 |
| MS.gene000883.t1 | AT3G06700 | 83.333 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 2.51e-30 | 100 |
| MS.gene000883.t1 | AT3G06700 | 83.333 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 2.51e-30 | 100 |
| MS.gene000883.t1 | AT3G06680 | 83.333 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 4.39e-30 | 100 |
| MS.gene000883.t1 | AT3G06680 | 83.333 | 60 | 10 | 0 | 1 | 60 | 1 | 60 | 4.39e-30 | 100 |
Find 13 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTGTGACGATGCCTCTT+TGG | 0.332537 | 7.2:-67824856 | None:intergenic |
| CTCTGGTTCCTCAAGAACTT+TGG | 0.404366 | 7.2:-67824991 | None:intergenic |
| TCTTGTGATCTTTGTAAGAC+TGG | 0.406426 | 7.2:-67824819 | None:intergenic |
| TATGCTTCCTTGCATACCTC+TGG | 0.420261 | 7.2:-67825008 | None:intergenic |
| ATCGTCACACTTCAACCAAA+GGG | 0.488627 | 7.2:+67824866 | MS.gene000883:CDS |
| ATGATGAATACATACCCCTT+TGG | 0.493133 | 7.2:-67824881 | None:intergenic |
| TCTTACAAAGATCACAAGAA+TGG | 0.554926 | 7.2:+67824823 | MS.gene000883:CDS |
| CATCGTCACACTTCAACCAA+AGG | 0.595920 | 7.2:+67824865 | MS.gene000883:CDS |
| AGATGGATCCAAAGTTCTTG+AGG | 0.596761 | 7.2:+67824983 | MS.gene000883:intron |
| TGAGGAACCAGAGGTATGCA+AGG | 0.624057 | 7.2:+67825001 | MS.gene000883:CDS |
| CAAAGTTCTTGAGGAACCAG+AGG | 0.651910 | 7.2:+67824992 | MS.gene000883:CDS |
| TTTGTAAGACTGGTTGTGAG+CGG | 0.659865 | 7.2:-67824809 | None:intergenic |
| TCGTCACACTTCAACCAAAG+GGG | 0.708509 | 7.2:+67824867 | MS.gene000883:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TCTTACAAAGATCACAAGAA+TGG | + | chr7.2:67824823-67824842 | MS.gene000883:CDS | 30.0% | |
| TTGTGATTGATTACTGTAGA+TGG | + | chr7.2:67824966-67824985 | MS.gene000883:intron | 30.0% | |
| ! | AGGAAGCATAACAAGAAAAA+TGG | + | chr7.2:67825021-67825040 | MS.gene000883:CDS | 30.0% |
| ATGATGAATACATACCCCTT+TGG | - | chr7.2:67824884-67824903 | None:intergenic | 35.0% | |
| TCTTGTGATCTTTGTAAGAC+TGG | - | chr7.2:67824822-67824841 | None:intergenic | 35.0% | |
| AGATGGATCCAAAGTTCTTG+AGG | + | chr7.2:67824983-67825002 | MS.gene000883:intron | 40.0% | |
| ATCGTCACACTTCAACCAAA+GGG | + | chr7.2:67824866-67824885 | MS.gene000883:CDS | 40.0% | |
| ATGGCATCAAAAAGCCAAAG+AGG | + | chr7.2:67824842-67824861 | MS.gene000883:CDS | 40.0% | |
| ! | TTTGTAAGACTGGTTGTGAG+CGG | - | chr7.2:67824812-67824831 | None:intergenic | 40.0% |
| CAAAGTTCTTGAGGAACCAG+AGG | + | chr7.2:67824992-67825011 | MS.gene000883:CDS | 45.0% | |
| CATCGTCACACTTCAACCAA+AGG | + | chr7.2:67824865-67824884 | MS.gene000883:CDS | 45.0% | |
| TATGCTTCCTTGCATACCTC+TGG | - | chr7.2:67825011-67825030 | None:intergenic | 45.0% | |
| TCGTCACACTTCAACCAAAG+GGG | + | chr7.2:67824867-67824886 | MS.gene000883:CDS | 45.0% | |
| ! | CTCTGGTTCCTCAAGAACTT+TGG | - | chr7.2:67824994-67825013 | None:intergenic | 45.0% |
| GAAGTGTGACGATGCCTCTT+TGG | - | chr7.2:67824859-67824878 | None:intergenic | 50.0% | |
| ! | TGAGGAACCAGAGGTATGCA+AGG | + | chr7.2:67825001-67825020 | MS.gene000883:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 67824787 | 67825065 | 67824787 | ID=MS.gene000883 |
| chr7.2 | mRNA | 67824787 | 67825065 | 67824787 | ID=MS.gene000883.t1;Parent=MS.gene000883 |
| chr7.2 | exon | 67824787 | 67824888 | 67824787 | ID=MS.gene000883.t1.exon1;Parent=MS.gene000883.t1 |
| chr7.2 | CDS | 67824787 | 67824888 | 67824787 | ID=cds.MS.gene000883.t1;Parent=MS.gene000883.t1 |
| chr7.2 | exon | 67824985 | 67825065 | 67824985 | ID=MS.gene000883.t1.exon2;Parent=MS.gene000883.t1 |
| chr7.2 | CDS | 67824985 | 67825065 | 67824985 | ID=cds.MS.gene000883.t1;Parent=MS.gene000883.t1 |
| Gene Sequence |
| Protein sequence |
