AlfalfaGEDB | MS.gene000884

M. sativa cultivar XinJiangDaYe / MS.gene000884

KEGG: K02905
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01779.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000884.t1	XP_004508161.1	98.4	61	1	0	1	61	1	61	3.40E-24	120.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000884.t1	Q9M7X7	83.6	61	10	0	1	61	1	61	8.8e-21	100.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000884.t1	I3SZB6	98.4	61	1	0	1	61	1	61	2.5e-24	120.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene043645	MS.gene000884	PPI
MS.gene043593	MS.gene000884	PPI
MS.gene064353	MS.gene000884	PPI
MS.gene000884	MS.gene043595	PPI
MS.gene043643	MS.gene000884	PPI
MS.gene000884	MS.gene73093	PPI
MS.gene035826	MS.gene000884	PPI
MS.gene064351	MS.gene000884	PPI
MS.gene035828	MS.gene000884	PPI
MS.gene000884	MS.gene035825	PPI
MS.gene99872	MS.gene000884	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000884.t1	MTR_4g121877	98.361	61	1	0	1	61	1	61	7.37e-39	122
MS.gene000884.t1	MTR_4g121870	93.443	61	4	0	1	61	137	197	1.26e-36	121

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000884.t1	AT3G06700	83.607	61	10	0	1	61	1	61	2.28e-31	103
MS.gene000884.t1	AT3G06700	83.607	61	10	0	1	61	1	61	2.28e-31	103
MS.gene000884.t1	AT3G06700	83.607	61	10	0	1	61	1	61	2.28e-31	103
MS.gene000884.t1	AT3G06680	83.607	61	10	0	1	61	23	83	2.54e-31	104
MS.gene000884.t1	AT3G06680	83.607	61	10	0	1	61	1	61	4.88e-31	102
MS.gene000884.t1	AT3G06680	83.607	61	10	0	1	61	1	61	4.88e-31	102

Find 14 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGTGTGGCGATGTCTCTT+TGG	0.360027	7.2:-67816635	None:intergenic
CTGATAAATACAAACTCCTT+TGG	0.392523	7.2:-67816660	None:intergenic
CTCTGGTTCCTCAAGAACTT+TGG	0.404366	7.2:-67816766	None:intergenic
TCTTGTGAGCCTTGTAAGAC+TGG	0.428046	7.2:-67816598	None:intergenic
TGTGCTTCCTTGCATACCTC+TGG	0.438138	7.2:-67816783	None:intergenic
CGCTCACAACCAGTCTTACA+AGG	0.489194	7.2:+67816589	MS.gene000884:CDS
ATAAATACAAACTCCTTTGG+TGG	0.508253	7.2:-67816657	None:intergenic
TCTTACAAGGCTCACAAGAA+TGG	0.521704	7.2:+67816602	MS.gene000884:CDS
AACAATTTACTCTTCTTCAG+TGG	0.567212	7.2:-67816827	None:intergenic
AGATGGATCCAAAGTTCTTG+AGG	0.579402	7.2:+67816758	MS.gene000884:intron
CATCGCCACACTTCCACCAA+AGG	0.596196	7.2:+67816644	MS.gene000884:CDS
AAACTCCTTTGGTGGAAGTG+TGG	0.614881	7.2:-67816649	None:intergenic
TGAGGAACCAGAGGTATGCA+AGG	0.624057	7.2:+67816776	MS.gene000884:CDS
CAAAGTTCTTGAGGAACCAG+AGG	0.651910	7.2:+67816767	MS.gene000884:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTATTTTTATAATTGTAGA+TGG	+	chr7.2:67816741-67816760	MS.gene000884:intron	10.0%
	ATAAATACAAACTCCTTTGG+TGG	-	chr7.2:67816660-67816679	None:intergenic	30.0%
	CTGATAAATACAAACTCCTT+TGG	-	chr7.2:67816663-67816682	None:intergenic	30.0%
!	AGGAAGCACAACAAGAAAAA+CGG	+	chr7.2:67816796-67816815	MS.gene000884:CDS	35.0%
	AGATGGATCCAAAGTTCTTG+AGG	+	chr7.2:67816758-67816777	MS.gene000884:intron	40.0%
	TCTTACAAGGCTCACAAGAA+TGG	+	chr7.2:67816602-67816621	MS.gene000884:CDS	40.0%
	AAACTCCTTTGGTGGAAGTG+TGG	-	chr7.2:67816652-67816671	None:intergenic	45.0%
	CAAAGTTCTTGAGGAACCAG+AGG	+	chr7.2:67816767-67816786	MS.gene000884:CDS	45.0%
!	CTCTGGTTCCTCAAGAACTT+TGG	-	chr7.2:67816769-67816788	None:intergenic	45.0%
!	TCTTGTGAGCCTTGTAAGAC+TGG	-	chr7.2:67816601-67816620	None:intergenic	45.0%
	CGCTCACAACCAGTCTTACA+AGG	+	chr7.2:67816589-67816608	MS.gene000884:CDS	50.0%
	GAAGTGTGGCGATGTCTCTT+TGG	-	chr7.2:67816638-67816657	None:intergenic	50.0%
	TGTGCTTCCTTGCATACCTC+TGG	-	chr7.2:67816786-67816805	None:intergenic	50.0%
!	TGAGGAACCAGAGGTATGCA+AGG	+	chr7.2:67816776-67816795	MS.gene000884:CDS	50.0%
	CATCGCCACACTTCCACCAA+AGG	+	chr7.2:67816644-67816663	MS.gene000884:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	67816566	67816843	67816566	ID=MS.gene000884
chr7.2	mRNA	67816566	67816843	67816566	ID=MS.gene000884.t1;Parent=MS.gene000884
chr7.2	exon	67816566	67816667	67816566	ID=MS.gene000884.t1.exon1;Parent=MS.gene000884.t1
chr7.2	CDS	67816566	67816667	67816566	ID=cds.MS.gene000884.t1;Parent=MS.gene000884.t1
chr7.2	exon	67816760	67816843	67816760	ID=MS.gene000884.t1.exon2;Parent=MS.gene000884.t1
chr7.2	CDS	67816760	67816843	67816760	ID=cds.MS.gene000884.t1;Parent=MS.gene000884.t1

Gene Sequence	>MS.gene000884 ATGGCAAAGTCGAAGAATCACATCGCTCACAACCAGTCTTACAAGGCTCACAAGAATGGCATCAAGAAGCCAAAGAGACATCGCCACACTTCCACCAAAGGAGTTTGTATTTATCAGTTTTCAATTCTTTTCTGAATTTGAAATGATTGATTTCTATTGATGATTAGTGATTTACTTTATTTTTATAATTGTAGATGGATCCAAAGTTCTTGAGGAACCAGAGGTATGCAAGGAAGCACAACAAGAAAAACGGTGAAATCGCCACTGAAGAAGAGTAA
Protein sequence	>MS.gene000884.t1 MAKSKNHIAHNQSYKAHKNGIKKPKRHRHTSTKGMDPKFLRNQRYARKHNKKNGEIATEEE

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