AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene001466


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001466.t1 MTR_3g102980 94.958 238 7 3 1 235 1 236 1.57e-150 418
MS.gene001466.t1 MTR_1g018420 55.102 245 83 8 1 233 1 230 5.89e-78 234
MS.gene001466.t1 MTR_1g106730 48.846 260 86 14 1 230 3 245 4.05e-56 179
MS.gene001466.t1 MTR_4g028610 30.693 202 72 6 30 163 134 335 4.72e-19 85.9
MS.gene001466.t1 MTR_0160s0030 40.152 132 53 4 84 215 240 345 5.99e-19 84.7
MS.gene001466.t1 MTR_4g027440 45.652 92 48 2 76 165 212 303 6.35e-19 84.7
MS.gene001466.t1 MTR_1g067650 30.769 169 90 3 58 209 1 159 2.16e-18 79.7
MS.gene001466.t1 MTR_1g067640 30.357 168 90 3 59 209 1 158 1.48e-17 77.4
MS.gene001466.t1 MTR_7g023560 34.375 128 70 2 52 179 12 125 1.03e-16 75.9
MS.gene001466.t1 MTR_1g090703 30.415 217 96 9 40 203 89 303 1.19e-16 78.2
MS.gene001466.t1 MTR_1g062780 31.683 202 81 7 52 207 81 271 2.36e-16 76.6
MS.gene001466.t1 MTR_5g099040 35.915 142 66 3 48 164 94 235 2.58e-16 76.3
MS.gene001466.t1 MTR_5g062300 39.130 138 73 3 38 164 71 208 3.71e-16 75.5
MS.gene001466.t1 MTR_1g115345 37.984 129 68 3 34 162 2 118 9.56e-16 73.6
MS.gene001466.t1 MTR_1g067660 33.529 170 85 5 47 209 6 154 2.86e-15 71.2
MS.gene001466.t1 MTR_7g100100 43.038 79 38 2 84 162 34 105 5.64e-15 70.5
MS.gene001466.t1 MTR_4g109340 38.514 148 49 8 85 200 349 486 2.68e-14 72.0
MS.gene001466.t1 MTR_2g096540 35.714 126 49 3 37 162 2 95 2.68e-14 67.8
MS.gene001466.t1 MTR_2g084315 29.907 214 96 6 41 200 60 273 7.33e-14 69.7
MS.gene001466.t1 MTR_4g100860 30.435 207 94 8 15 200 124 301 2.21e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001466.t1 AT1G27730 51.899 237 96 7 1 231 1 225 3.56e-62 194
MS.gene001466.t1 AT5G04340 49.576 236 98 10 1 222 1 229 1.53e-55 177
MS.gene001466.t1 AT3G19580 45.802 262 102 10 1 227 1 257 4.78e-50 165
MS.gene001466.t1 AT3G19580 45.802 262 102 10 1 227 1 257 4.78e-50 165
MS.gene001466.t1 AT5G43170 48.718 234 70 11 1 226 1 192 5.39e-46 152
MS.gene001466.t1 AT3G49930 49.143 175 68 5 30 200 36 193 9.84e-40 136
MS.gene001466.t1 AT5G67450 41.250 240 98 8 1 200 1 237 1.76e-39 137
MS.gene001466.t1 AT2G45120 40.237 169 78 5 44 200 143 300 2.88e-24 98.6
MS.gene001466.t1 AT3G60580 37.209 172 79 4 44 200 119 276 8.76e-24 97.1
MS.gene001466.t1 AT2G28200 33.178 214 105 7 44 220 68 280 1.05e-22 94.0
MS.gene001466.t1 AT1G49900 51.579 95 40 2 85 178 194 283 8.58e-22 94.0
MS.gene001466.t1 AT1G49900 40.000 135 72 3 83 217 749 874 6.36e-18 82.8
MS.gene001466.t1 AT5G56200 47.423 97 38 2 84 167 342 438 1.42e-20 90.1
MS.gene001466.t1 AT5G03510 33.166 199 93 5 43 203 64 260 5.29e-19 84.0
MS.gene001466.t1 AT1G02040 31.771 192 94 8 5 163 61 248 1.68e-18 83.2
MS.gene001466.t1 AT2G37430 38.095 126 67 2 37 162 2 116 7.73e-17 75.9
MS.gene001466.t1 AT4G35280 38.400 125 53 4 48 164 138 246 1.20e-16 77.4
MS.gene001466.t1 AT1G02030 34.194 155 79 4 47 200 123 255 2.74e-16 76.3
MS.gene001466.t1 AT2G17180 30.625 160 76 5 48 200 121 252 7.77e-16 75.1
MS.gene001466.t1 AT3G46080 40.171 117 46 3 49 162 12 107 1.13e-15 72.4
MS.gene001466.t1 AT3G46090 36.923 130 62 3 39 167 3 113 1.79e-15 72.0
MS.gene001466.t1 AT5G59820 37.815 119 52 3 55 173 18 114 2.72e-15 71.2
MS.gene001466.t1 AT5G04390 28.889 225 90 7 48 203 96 319 6.23e-15 73.6
MS.gene001466.t1 AT3G53600 34.127 126 71 2 37 162 2 115 8.45e-15 70.5
MS.gene001466.t1 AT2G28710 39.640 111 43 3 52 162 14 100 5.33e-14 67.8
MS.gene001466.t1 AT3G10470 38.235 102 40 4 84 162 182 283 1.73e-12 66.6
MS.gene001466.t1 AT3G46070 34.545 110 55 3 53 162 16 108 2.24e-12 63.5
MS.gene001466.t1 AT5G61470 37.179 78 39 2 86 163 230 297 9.15e-11 60.8

Find 63 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCCTACTGGACAGGCTTT+GGG 0.284852 3.4:+86127446 MS.gene001466:CDS
TTGCGGACATAACAGCTTTC+CGG 0.302034 3.4:-86127319 None:intergenic
CTTCCCTACTGGACAGGCTT+TGG 0.307779 3.4:+86127445 MS.gene001466:CDS
GTTTCAAGATAACTCAGATT+TGG 0.369889 3.4:-86127062 None:intergenic
TACTCCTTTCACACCCTTTG+AGG 0.385893 3.4:+86127037 MS.gene001466:CDS
GGCAGCGTTGGTGCCGGTGC+CGG 0.399502 3.4:+86127494 MS.gene001466:CDS
CTCAGATTTGGTTCCTCAAA+GGG 0.424837 3.4:-86127050 None:intergenic
GTTGGTGCCGGTGCCGGTGC+TGG 0.425482 3.4:+86127500 MS.gene001466:CDS
CATAACAGCTTTCCGGTGAC+TGG 0.439265 3.4:-86127312 None:intergenic
GGCTTCTTTGCAGCAGGTAA+AGG 0.446061 3.4:-86127650 None:intergenic
AATTTCCAGCTTGAAAAGAC+AGG 0.452679 3.4:-86127672 None:intergenic
CTGATCTTCAGCGGTGGTTG+CGG 0.460016 3.4:-86127336 None:intergenic
TTTGAAAAGTCCGGAAAAGC+CGG 0.466243 3.4:-86127596 None:intergenic
ACTGGCTTTGTGTCCACCTA+GGG 0.475209 3.4:-86127294 None:intergenic
GATCGTTTACCTTTCGTCCA+CGG 0.485045 3.4:-86127086 None:intergenic
CTTACCGTTGGAAGCAGAGC+TGG 0.488264 3.4:-86127387 None:intergenic
GCATTTGTGACTGAGTTTAA+CGG 0.491121 3.4:-86127237 None:intergenic
ATGAGTCTTGTTCTTACCGT+TGG 0.503655 3.4:-86127399 None:intergenic
TGTCACAAATCCTTCCCTAC+TGG 0.506283 3.4:+86127434 MS.gene001466:CDS
AGACAGGGCTTCTTTGCAGC+AGG 0.516606 3.4:-86127656 None:intergenic
GGTTCCTCAAAGGGTGTGAA+AGG 0.517530 3.4:-86127041 None:intergenic
ACGATCAAAGCGTTCTCGCA+TGG 0.518878 3.4:+86127103 MS.gene001466:CDS
TACGAAGGCAGCGTTGGTGC+CGG 0.519961 3.4:+86127488 MS.gene001466:CDS
TTGAAAAGTCCGGAAAAGCC+GGG 0.523321 3.4:-86127595 None:intergenic
GACTGGCTTTGTGTCCACCT+AGG 0.526559 3.4:-86127295 None:intergenic
CAAATCCTTCCCTACTGGAC+AGG 0.527321 3.4:+86127439 MS.gene001466:CDS
CTGAGTTTAACGGTGGTTAG+CGG 0.533899 3.4:-86127227 None:intergenic
CCTCCCAAAGCCTGTCCAGT+AGG 0.538116 3.4:-86127449 None:intergenic
ACTCAGATTTGGTTCCTCAA+AGG 0.539745 3.4:-86127051 None:intergenic
AGGGAAGGATTTGTGACAGA+TGG 0.550231 3.4:-86127429 None:intergenic
TTCTCATCTTATCAAGCCCT+AGG 0.550654 3.4:+86127278 MS.gene001466:CDS
GTAACTGCCTCTGAAGGAGT+TGG 0.551176 3.4:+86127533 MS.gene001466:CDS
CTCCCAAAGCCTGTCCAGTA+GGG 0.562622 3.4:-86127448 None:intergenic
CTTGAAACACCGTGGACGAA+AGG 0.563216 3.4:+86127077 MS.gene001466:CDS
TGTGAAAGGAGTAGCAGTGG+TGG 0.569120 3.4:-86127027 None:intergenic
TGTCACTACGAAGGCAGCGT+TGG 0.571767 3.4:+86127482 MS.gene001466:CDS
GGTAGTCACGGCGGATGAAG+TGG 0.575582 3.4:-86127366 None:intergenic
GCTACTTCCAGCACCGGCAC+CGG 0.578492 3.4:-86127507 None:intergenic
TAAGATCAAAATCACGGTGG+TGG 0.583762 3.4:-86127568 None:intergenic
CAAAGCCTGTCCAGTAGGGA+AGG 0.583815 3.4:-86127444 None:intergenic
GGAAGCAGAGCTGGTAGTCA+CGG 0.585255 3.4:-86127378 None:intergenic
CTCATCATGCTTGCTCGCAG+CGG 0.585296 3.4:+86127167 MS.gene001466:CDS
GTGGACACAAAGCCAGTCAC+CGG 0.585980 3.4:+86127300 MS.gene001466:CDS
ATTTCCAGCTTGAAAAGACA+GGG 0.591447 3.4:-86127671 None:intergenic
TACAGCGCTACTTCCAGCAC+CGG 0.591699 3.4:-86127513 None:intergenic
ATTATTGATCAGTAATGAGA+AGG 0.592327 3.4:-86127695 None:intergenic
GTGAAAGGAGTAGCAGTGGT+GGG 0.592634 3.4:-86127026 None:intergenic
AGCGCTGTAACTGCCTCTGA+AGG 0.607763 3.4:+86127527 MS.gene001466:CDS
CCTACTGGACAGGCTTTGGG+AGG 0.612476 3.4:+86127449 MS.gene001466:CDS
TCATCTTATCAAGCCCTAGG+TGG 0.625860 3.4:+86127281 MS.gene001466:CDS
TGACGATCCAACTCCTTCAG+AGG 0.633382 3.4:-86127540 None:intergenic
GGGTGTGAAAGGAGTAGCAG+TGG 0.640901 3.4:-86127030 None:intergenic
ACTACCAGCTCTGCTTCCAA+CGG 0.645257 3.4:+86127383 MS.gene001466:CDS
GGAGGTTAAGATCAAAATCA+CGG 0.658091 3.4:-86127574 None:intergenic
AGTCACGGCGGATGAAGTGG+TGG 0.674100 3.4:-86127363 None:intergenic
CACAAGCGTTGTCACTACGA+AGG 0.677003 3.4:+86127473 MS.gene001466:CDS
TTTGTGACTGAGTTTAACGG+TGG 0.692042 3.4:-86127234 None:intergenic
AGCAGAGCTGGTAGTCACGG+CGG 0.700777 3.4:-86127375 None:intergenic
GGTGATCTGATCTTCAGCGG+TGG 0.700953 3.4:-86127342 None:intergenic
GGTGGTGATCTGATCTTCAG+CGG 0.704088 3.4:-86127345 None:intergenic
AAAAGTCCGGAAAAGCCGGG+AGG 0.720865 3.4:-86127592 None:intergenic
TGAGTTATCTTGAAACACCG+TGG 0.739154 3.4:+86127069 MS.gene001466:CDS
GGTTAAGATCAAAATCACGG+TGG 0.807668 3.4:-86127571 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACTTCTTTGAAAAGTC+CGG - chr3.4:86127608-86127627 None:intergenic 25.0%
GTTTCAAGATAACTCAGATT+TGG - chr3.4:86127065-86127084 None:intergenic 30.0%
! CTTTTCAAAGAAGTTTTTCG+TGG + chr3.4:86127610-86127629 MS.gene001466:CDS 30.0%
AATTTCCAGCTTGAAAAGAC+AGG - chr3.4:86127675-86127694 None:intergenic 35.0%
ATTTCCAGCTTGAAAAGACA+GGG - chr3.4:86127674-86127693 None:intergenic 35.0%
CAACGACAAAAAGTCTGATT+CGG + chr3.4:86127196-86127215 MS.gene001466:CDS 35.0%
GCATTTGTGACTGAGTTTAA+CGG - chr3.4:86127240-86127259 None:intergenic 35.0%
GGAGGTTAAGATCAAAATCA+CGG - chr3.4:86127577-86127596 None:intergenic 35.0%
!! TGATTTTGATCTTAACCTCC+CGG + chr3.4:86127577-86127596 MS.gene001466:CDS 35.0%
GGTTAAGATCAAAATCACGG+TGG - chr3.4:86127574-86127593 None:intergenic 40.0%
TAAGATCAAAATCACGGTGG+TGG - chr3.4:86127571-86127590 None:intergenic 40.0%
TGAGTTATCTTGAAACACCG+TGG + chr3.4:86127069-86127088 MS.gene001466:CDS 40.0%
TTCTCATCTTATCAAGCCCT+AGG + chr3.4:86127278-86127297 MS.gene001466:CDS 40.0%
TTTGAAAAGTCCGGAAAAGC+CGG - chr3.4:86127599-86127618 None:intergenic 40.0%
TTTGTGACTGAGTTTAACGG+TGG - chr3.4:86127237-86127256 None:intergenic 40.0%
! ACTCAGATTTGGTTCCTCAA+AGG - chr3.4:86127054-86127073 None:intergenic 40.0%
! CTCAGATTTGGTTCCTCAAA+GGG - chr3.4:86127053-86127072 None:intergenic 40.0%
!! ATGAGTCTTGTTCTTACCGT+TGG - chr3.4:86127402-86127421 None:intergenic 40.0%
AGGGAAGGATTTGTGACAGA+TGG - chr3.4:86127432-86127451 None:intergenic 45.0%
CTGAGTTTAACGGTGGTTAG+CGG - chr3.4:86127230-86127249 None:intergenic 45.0%
GATCGTTTACCTTTCGTCCA+CGG - chr3.4:86127089-86127108 None:intergenic 45.0%
TACTCCTTTCACACCCTTTG+AGG + chr3.4:86127037-86127056 MS.gene001466:CDS 45.0%
TCATCTTATCAAGCCCTAGG+TGG + chr3.4:86127281-86127300 MS.gene001466:CDS 45.0%
TGTCACAAATCCTTCCCTAC+TGG + chr3.4:86127434-86127453 MS.gene001466:CDS 45.0%
TTGAAAAGTCCGGAAAAGCC+GGG - chr3.4:86127598-86127617 None:intergenic 45.0%
TTGCGGACATAACAGCTTTC+CGG - chr3.4:86127322-86127341 None:intergenic 45.0%
! GAAGTTTTTCGTGGATGACG+AGG + chr3.4:86127619-86127638 MS.gene001466:CDS 45.0%
!! CGACAAAAAGTCTGATTCGG+TGG + chr3.4:86127199-86127218 MS.gene001466:CDS 45.0%
ACTACCAGCTCTGCTTCCAA+CGG + chr3.4:86127383-86127402 MS.gene001466:CDS 50.0%
CAAATCCTTCCCTACTGGAC+AGG + chr3.4:86127439-86127458 MS.gene001466:CDS 50.0%
CATAACAGCTTTCCGGTGAC+TGG - chr3.4:86127315-86127334 None:intergenic 50.0%
CTTGAAACACCGTGGACGAA+AGG + chr3.4:86127077-86127096 MS.gene001466:CDS 50.0%
GGCTTCTTTGCAGCAGGTAA+AGG - chr3.4:86127653-86127672 None:intergenic 50.0%
GGTGGTGATCTGATCTTCAG+CGG - chr3.4:86127348-86127367 None:intergenic 50.0%
GTGAAAGGAGTAGCAGTGGT+GGG - chr3.4:86127029-86127048 None:intergenic 50.0%
TGACGATCCAACTCCTTCAG+AGG - chr3.4:86127543-86127562 None:intergenic 50.0%
TGTGAAAGGAGTAGCAGTGG+TGG - chr3.4:86127030-86127049 None:intergenic 50.0%
TTCCCTACTGGACAGGCTTT+GGG + chr3.4:86127446-86127465 MS.gene001466:CDS 50.0%
! ACGATCAAAGCGTTCTCGCA+TGG + chr3.4:86127103-86127122 MS.gene001466:CDS 50.0%
! CACAAGCGTTGTCACTACGA+AGG + chr3.4:86127473-86127492 MS.gene001466:CDS 50.0%
! GGTTCCTCAAAGGGTGTGAA+AGG - chr3.4:86127044-86127063 None:intergenic 50.0%
! GTAACTGCCTCTGAAGGAGT+TGG + chr3.4:86127533-86127552 MS.gene001466:CDS 50.0%
! TCTTAACCTCCCGGCTTTTC+CGG + chr3.4:86127586-86127605 MS.gene001466:CDS 50.0%
!! ACTGGCTTTGTGTCCACCTA+GGG - chr3.4:86127297-86127316 None:intergenic 50.0%
!! GAAGCCCTGTCTTTTCAAGC+TGG + chr3.4:86127667-86127686 MS.gene001466:CDS 50.0%
AAAAGTCCGGAAAAGCCGGG+AGG - chr3.4:86127595-86127614 None:intergenic 55.0%
AGACAGGGCTTCTTTGCAGC+AGG - chr3.4:86127659-86127678 None:intergenic 55.0%
AGCGCTGTAACTGCCTCTGA+AGG + chr3.4:86127527-86127546 MS.gene001466:CDS 55.0%
CAAAGCCTGTCCAGTAGGGA+AGG - chr3.4:86127447-86127466 None:intergenic 55.0%
CTCATCATGCTTGCTCGCAG+CGG + chr3.4:86127167-86127186 MS.gene001466:CDS 55.0%
CTCCCAAAGCCTGTCCAGTA+GGG - chr3.4:86127451-86127470 None:intergenic 55.0%
CTGATCTTCAGCGGTGGTTG+CGG - chr3.4:86127339-86127358 None:intergenic 55.0%
CTTACCGTTGGAAGCAGAGC+TGG - chr3.4:86127390-86127409 None:intergenic 55.0%
CTTCCCTACTGGACAGGCTT+TGG + chr3.4:86127445-86127464 MS.gene001466:CDS 55.0%
GGAAGCAGAGCTGGTAGTCA+CGG - chr3.4:86127381-86127400 None:intergenic 55.0%
GGGTGTGAAAGGAGTAGCAG+TGG - chr3.4:86127033-86127052 None:intergenic 55.0%
GGTGATCTGATCTTCAGCGG+TGG - chr3.4:86127345-86127364 None:intergenic 55.0%
GTGGACACAAAGCCAGTCAC+CGG + chr3.4:86127300-86127319 MS.gene001466:CDS 55.0%
TACAGCGCTACTTCCAGCAC+CGG - chr3.4:86127516-86127535 None:intergenic 55.0%
TGTCACTACGAAGGCAGCGT+TGG + chr3.4:86127482-86127501 MS.gene001466:CDS 55.0%
!! GACTGGCTTTGTGTCCACCT+AGG - chr3.4:86127298-86127317 None:intergenic 55.0%
AGCAGAGCTGGTAGTCACGG+CGG - chr3.4:86127378-86127397 None:intergenic 60.0%
AGTCACGGCGGATGAAGTGG+TGG - chr3.4:86127366-86127385 None:intergenic 60.0%
CCTCCCAAAGCCTGTCCAGT+AGG - chr3.4:86127452-86127471 None:intergenic 60.0%
GGTAGTCACGGCGGATGAAG+TGG - chr3.4:86127369-86127388 None:intergenic 60.0%
!! CCTACTGGACAGGCTTTGGG+AGG + chr3.4:86127449-86127468 MS.gene001466:CDS 60.0%
!! TACGAAGGCAGCGTTGGTGC+CGG + chr3.4:86127488-86127507 MS.gene001466:CDS 60.0%
!! GCTACTTCCAGCACCGGCAC+CGG - chr3.4:86127510-86127529 None:intergenic 65.0%
!! GGCAGCGTTGGTGCCGGTGC+CGG + chr3.4:86127494-86127513 MS.gene001466:CDS 75.0%
!! GTTGGTGCCGGTGCCGGTGC+TGG + chr3.4:86127500-86127519 MS.gene001466:CDS 75.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 86127002 86127709 86127002 ID=MS.gene001466
chr3.4 mRNA 86127002 86127709 86127002 ID=MS.gene001466.t1;Parent=MS.gene001466
chr3.4 exon 86127002 86127709 86127002 ID=MS.gene001466.t1.exon1;Parent=MS.gene001466.t1
chr3.4 CDS 86127002 86127709 86127002 ID=cds.MS.gene001466.t1;Parent=MS.gene001466.t1
Gene Sequence

>MS.gene001466

ATGGCTATGGAAGCACTTAACTCACCCACCACTGCTACTCCTTTCACACCCTTTGAGGAACCAAATCTGAGTTATCTTGAAACACCGTGGACGAAAGGTAAACGATCAAAGCGTTCTCGCATGGATCAATCTTCATGCACTGAAGAAGAGTATCTCGCTCTTTGTCTCATCATGCTTGCTCGCAGCGGTAACAACAACGACAAAAAGTCTGATTCGGTGGCGACGCCGCTAACCACCGTTAAACTCAGTCACAAATGCTCAGTCTGCAACAAAGCTTTCTCATCTTATCAAGCCCTAGGTGGACACAAAGCCAGTCACCGGAAAGCTGTTATGTCCGCAACCACCGCTGAAGATCAGATCACCACCACTTCATCCGCCGTGACTACCAGCTCTGCTTCCAACGGTAAGAACAAGACTCATGAGTGTTCCATCTGTCACAAATCCTTCCCTACTGGACAGGCTTTGGGAGGACACAAGCGTTGTCACTACGAAGGCAGCGTTGGTGCCGGTGCCGGTGCTGGAAGTAGCGCTGTAACTGCCTCTGAAGGAGTTGGATCGTCACACAGCCACCACCGTGATTTTGATCTTAACCTCCCGGCTTTTCCGGACTTTTCAAAGAAGTTTTTCGTGGATGACGAGGTTTCTAGTCCTTTACCTGCTGCAAAGAAGCCCTGTCTTTTCAAGCTGGAAATTCCTTCTCATTACTGA

Protein sequence

>MS.gene001466.t1

MAMEALNSPTTATPFTPFEEPNLSYLETPWTKGKRSKRSRMDQSSCTEEEYLALCLIMLARSGNNNDKKSDSVATPLTTVKLSHKCSVCNKAFSSYQALGGHKASHRKAVMSATTAEDQITTTSSAVTTSSASNGKNKTHECSICHKSFPTGQALGGHKRCHYEGSVGAGAGAGSSAVTASEGVGSSHSHHRDFDLNLPAFPDFSKKFFVDDEVSSPLPAAKKPCLFKLEIPSHY